CN108064887A - Application of the spiderflower in the activity for inhibiting plant soil-borne diseases pathogenic bacteria - Google Patents
Application of the spiderflower in the activity for inhibiting plant soil-borne diseases pathogenic bacteria Download PDFInfo
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- CN108064887A CN108064887A CN201810047279.7A CN201810047279A CN108064887A CN 108064887 A CN108064887 A CN 108064887A CN 201810047279 A CN201810047279 A CN 201810047279A CN 108064887 A CN108064887 A CN 108064887A
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- spiderflower
- cucumber fusarium
- fusarium axysporum
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- paeonol
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The present invention relates to application of the spiderflower in plant soil-borne diseases pathogenic activity is inhibited.Present invention firstly discovers that spiderflower can be used for preventing plant soil-borne diseases, caused cucumber fusarium axysporum is especially infected by asexual mushroom Fusarium Fusarium oxysporum cucumber specialized form (Fusarium oxysporium f.sp.cucumarinum), spiderflower can be used as a kind of Biofumigation agent, there is the problems such as environmental protection is effective, environmental pollution caused by avoiding chemical pesticide and pesticide residue.
Description
Technical field
The invention belongs to field of biological control more particularly to spiderflower in the activity for inhibiting plant soil-borne diseases pathogenic bacteria
Application.
Background technology
By asexual mushroom Fusarium Fusarium oxysporum cucumber specialized form (Fusarium oxysporium
F.sp.cucumarinum cucumber fusarium axysporum caused by) infecting is the important soil-borne disease in cucumber production, due to protecting field
Special environment condition and long-term continuous cropping, the accumulation of pathogen is more and more in soil, causes the soil-borne diseases such as cucumber fusarium axysporum
Generation getting worse.Incidence is generally 10%-30%, up to 80%-90% when serious, causes heavy losses.Use chemistry
It is the effective way for preventing the soil-borne diseases such as cucumber fusarium axysporum that medicament, which carries out disinfection to soil and fumigates,.As chemical pesticide causes
Environmental pollution it is increasingly severe and the mankind are more and more stronger to the demand of " pollution-free food ", finding environmental protection can effectively replace
Biofumigation agent for chemical agent becomes the task of top priority.
Brassica plants such as radish, leaf mustard and cauliflower etc. contain substantial amounts of glucosinolate, can generate a series of volatilizations
Property or non-volatile bioactive substance include the isothiocyanates of oxazolidine sulphur, ketone, thiocyanates and different structure, to soil
Plant nematode in earth, weeds disease fungus, have Biofumigation effect, and can improve soil texture and crop yield.It is liquor-saturated
Butterfly spends (Cleome spinosa) also known as purple dragon, Western cabbage rolls, swallowtail butterfly grass, spider flower, is that cabbage rolls section spiderflower belongs to
First, biennial herb plant.But there is no the relevant report that Biofumigation is carried out using spiderflower both at home and abroad at present, spiderflower is
It is no to there is bacteriostatic activity also to have not proved out soil-borne disease.
The content of the invention
In view of the problems of prior art, the present invention provides a kind of spiderflower and is inhibiting plant soil-borne diseases pathogenic bacteria
Activity in application.
The technical solution that the present invention solves above-mentioned technical problem is as follows：
The present invention provides spiderflower and can be applied in the activity for inhibiting plant soil-borne diseases pathogenic bacteria for the first time.This hair
It is bright further to provide new thinking using Biofumigation technology prevention soil-borne disease, spiderflower is a kind of effective biology of environmental protection
Fumigant has many advantages, such as the problems such as environmental protection is effective, environmental pollution caused by avoiding chemical pesticide and pesticide residue.
Further, present invention firstly discovers that spiderflower can be in the application in the activity for inhibiting cucumber fusarium axysporum.It is liquor-saturated
Butterfly spends the prevention that cucumber fusarium axysporum can be used for as Biofumigation agent.
Further, spiderflower contains the mixing of paeonol, linalool and tea perfume spirane one or more
The beneficial effects of the invention are as follows：The present invention has studied spiderflower cauline leaf crushed material and cucumber fusarium axysporum is smoked for the first time
Steaming acts on, and specifies bacteriostasis and ingredient of the spiderflower to cucumber fusarium axysporum, further to utilize Biofumigation technology prevention
Soil-borne disease provides new thinking, has many advantages, such as environmentally friendly effective, environmental pollution and pesticide caused by avoiding chemical pesticide
The problems such as residual.
Application of the spiderflower volatile ingredient extract in the activity for inhibiting cucumber fusarium axysporum, the spiderflower are volatilized into
Extract is divided to include the mixing of paeonol, linalool and tea perfume spirane one or more.The volatile ingredient extract of spiderflower can
Prevention to be used for cucumber fusarium axysporum as Biofumigation agent.
The beneficial effects of the invention are as follows：The present invention using GC/MS have detected spiderflower volatile materials active ingredient and
The bacteriostatic activity of each ingredient provides a kind of new Biofumigation agent, has many advantages, such as that environmental protection is effective, avoids chemical pesticide
Caused by environmental pollution and the problems such as pesticide residue.
It has also been found that paeonol, aromatic alcohol or tea perfume spirane can inhibit the side such as activity of cucumber fusarium axysporum
It is applied in face.Paeonol, aromatic alcohol or tea perfume spirane can be used for the prevention of cucumber fusarium axysporum as Biofumigation agent.
Inventor is found surprisingly that tea perfume spirane has cucumber fusarium axysporum significant inhibitory action, antibacterial under study for action
Rate can reach 91.0%, and the bacteriostasis rate that the bacteriostasis rate of paeonol can reach 92.6%, aromatic alcohol can reach 98.4%, tool
Have the advantages that environmental protection is effective.
A kind of method for the activity for being inhibited cucumber fusarium axysporum using spiderflower, is comprised the following steps：By spiderflower cauline leaf
Biofumigation is carried out to cucumber fusarium axysporum after crushing.Concrete operations can be：Spiderflower cauline leaf is crushed into after-applied cultivating
Ware ware covers, and cucumber fusarium axysporum bacterium dish has been placed at PSA culture medium centrals.
The beneficial effects of the invention are as follows：Prevention to cucumber fusarium axysporum can be realized by the above method, had with environmental protection
The advantages that effect.
Further, acquisition grows the spiderflower cauline leaf of 120-150 days.
A kind of method for inhibiting the activity of cucumber fusarium axysporum using spiderflower volatile ingredient extract, including following step
Suddenly：Using steam distillation obtain spiderflower volatile ingredient extract, the spiderflower volatile ingredient extract contain paeonol,
Spiderflower volatile ingredient extract is carried out biology by the mixing of linalool and tea perfume spirane one or more to cucumber fusarium axysporum
It is stifling.Concrete operations can be：Spiderflower volatile ingredient extract, the spiderflower volatile ingredient are obtained using steam distillation
Extract contains the mixing of paeonol, linalool and tea perfume spirane one or more, and spiderflower volatile ingredient extract is applied
It is covered in culture dish ware, cucumber fusarium axysporum bacterium dish has been placed at PSA culture medium centrals.
The beneficial effects of the invention are as follows：Prevention to cucumber fusarium axysporum can be realized by the above method, had with environmental protection
The advantages that effect.
It is a kind of using paeonol, aromatic alcohol or tea perfume spirane come inhibit cucumber fusarium axysporum activity method, including with
Lower step：Paeonol, aromatic alcohol or tea perfume spirane are subjected to Biofumigation to cucumber fusarium axysporum.Concrete operations can be：It will
Paeonol, aromatic alcohol or tea perfume spirane are applied to culture dish ware and cover, and cucumber fusarium axysporum bacterium dish has been placed in PSA culture mediums
Place.
The beneficial effects of the invention are as follows：Prevention to cucumber fusarium axysporum can be realized by the above method, had with environmental protection
The advantages that effect.
Description of the drawings
Fig. 1 is fumigating effect figure of the various dose spiderflower crushed material to cucumber fusarium axysporum.
Fig. 2 is graph of the various dose spiderflower to the fumigating effect of cucumber fusarium axysporum.
Fig. 3 is spiderflower GC/MS total ion chromatograms.
Fig. 4 for 8 kinds of spiderflower volatile materials to the fungistatic effect of cucumber fusarium axysporum, A is blank control；B is 14
Alkyl epoxy ethane；C is lignocerane；D is heptadecane；E is octacosane；F is ethylcyclopentenolone；G is tea perfume spirane；
H is linalool；I is paeonol.
Fig. 5 be 3 kinds of tea perfume spirane, linalool and paeonol substances to the fungistatic effect of cucumber fusarium axysporum, wherein, A is
Tea perfume spirane is to the fungistatic effect of cucumber fusarium axysporum.A1 is blank control；A2 is 0.0075g/ wares；A3 is 0.0151g/ wares；A4
For 0.0226g/ wares；A5 is 0.0301g/ wares.B is fungistatic effect of the paeonol to cucumber fusarium axysporum.B1 is blank control；B2 is
0.0678g/ wares；B3 is 0.1356g/ wares；B4 is 0.2034g/ wares；B5 is 0.2712g/ wares.C is linalool to cucumber fusarium axysporum
Fungistatic effect.C1 is blank control；C2 is 0.0112g/ wares；C3 is 0.0224g/ wares；C4 is 0.0337g/ wares；C5 is
0.0449g/ wares.
Fig. 6 is various dose tea perfume spirane, linalool and paeonol to the inhibition of wilt, the agent of tea perfume spirane
Amount is respectively per ware 0g, 0.0075g, 0.0151g, 0.0226g, 0.0301g；The dosage of paeonol be respectively 0g, 0.0678g,
0.1356g、0.2034g、0.2712g.The dosage of linalool is respectively 0g, 0.0112g, 0.0224g, 0.0337g, 0.0449g.
Specific embodiment
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
To find the Biofumigation agent of effective, the alternative chemical agent of environmental protection, the present invention is ground using mycelial growth rate method
Fumigation action of the spiderflower cauline leaf crushed material to cucumber fusarium axysporum is studied carefully, spiderflower volatile materials is had detected using GC/MS
Active ingredient and each ingredient bacteriostatic activity.Result of study shows the spiderflower of 0.1g, 0.2g, 0.3g and 0.4g4 dosage
Crushed material has inhibitory action to cucumber fusarium axysporum, wherein per ware 0.3g and 0.4g bacteriostasis rate up to 100%.GC/MS is identified
Go out 21 kinds of spiderflower volatile materials, the fumigating effect of wherein 8 kinds ingredients is studied, the results showed that paeonol, linalool
There is inhibitory action to cucumber fusarium axysporum with tea perfume spirane, bacteriostasis rate is respectively 92.6%, 98.4% and 91.0%, and the tetradecane
Hexadecyl ethylene oxide, lignocerane, heptadecane, ethylcyclopentenolone and octacosane make the equal unrestraint of cucumber fusarium axysporum
With.
It is introduced below by specific embodiment.
In each embodiment, cucumber fusarium axysporum (FOC, Fusarium oxysporum f.sp.cucumerinum), by east
Northern agriculture university's plant pathology research department provides, and can be that the public obtains, be repeated for non-commercial purpose in the experiment of the application
Hold.The bacterial strain was once documented in the specification part of patent of invention (Patent No. ZL201110400011.5), was specifically shown in the explanation
" preparation of bacteria suspension " of the step of method I of the embodiment one of book one.
Spiderflower (Cleome spinosa) seed is purchased from Harbin flowers market, and conventional method cultivation is big in northeast agricultural
In school garden.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified
1 spiderflower of embodiment is to the bacteriostasis of cucumber fusarium axysporum
Inhibition of the spiderflower to cucumber fusarium axysporum mycelia is measured using mycelial growth rate method.Steam distillation
It is referred to document：Zhang Xinhu, He Jing, Shen Huimin wait inhibitory action and antibacterial machine of the Siberian cockleburs extract to botrytis cinerea
Manage pre-test [J] Acta Pratacultures, 2008,17 (3)：99-104.
Concrete operations are:The fresh spiderflower cauline leaf of acquisition growth 50d or so, cleans up and dries, with Chinese herbal medicine height
It is spare that fast pulverizer is crushed to fine-powdered.The cucumber fusarium axysporum of 7d will be cultivated on PSA culture mediums, with a diameter of 0.85cm's
Card punch cuts bacteria cake in colony edge punching, with bacterium hook is moved that pure culture biscuits involvng inoculation is central in the 9.0cm culture dishes for having PSA,
Culture dish ware covers the spiderflower crushed material for being separately added into 0g, 0.1g, 0.2g, 0.3g, 0.4g various dose, is sealed with sealed membrane
Mouthful, it is placed in dark culturing in 20 DEG C of incubators.5 repetitions are often handled, each bacterium colony is vertically surveyed with crossing method every for 24 hours
Diameter is measured, takes its average value.Spiderflower is calculated to the antibacterial of cucumber fusarium axysporum according to the result measured using following formula
Rate.
Fig. 1 and Fig. 2 is fumigating effect of the various dose spiderflower crushed material to cucumber fusarium axysporum.In Fig. 1, A is blank
Control, B are 0.1g/ wares, and C is 0.2g/ wares, and D is 0.3g/ wares, and D is 0.4g/ wares.From Fig. 1 and Fig. 2 as it can be seen that 4 dosage it is liquor-saturated
Butterfly pollen minces and has inhibition to cucumber fusarium axysporum.Blank control (CK) a diameter of 2.917cm of bacterium colony balanced growth,
The a diameter of 1.986cm of bacterium colony balanced growth after 0.1g spiderflower crushed material suffocating treatments, bacteriostasis rate 32.2%；0.2g spiderflowers
Handle a diameter of 1.313cm of bacterium colony balanced growth, bacteriostasis rate 54.8%；0.3g and 0.4g bacterium colony balanced growth diameters are
0cm, bacteriostasis rate are 100%.
The experimental results showed that：Fumigation action of the spiderflower to cucumber fusarium axysporum.
2 spiderflower volatile materials constituent analysis of embodiment
The extraction of spiderflower volatile materials uses steam distillation.Steam distillation may be referred to document：Li Xiao
It is bright, Li Chengchong, an ox outstanding person, treasure's traditional Chinese medical science traditional Chinese medicines as orthogonal experiments Optimization of Extraction Process of Acorus tatarinowii Naphtha [J], 2011,22
(4)：939.
Concrete operation method is：50g spiderflower cauline leafs are weighed, are crushed with medicinal herb grinder, is placed in 500mL flasks, adds
Distilled water after mixing, adds in 35g NaCl.Volatile oil extractor with reflux condensing tube is connected, adds in and steams from condenser pipe upper end
Until distilled water makes the scale part full of volatile oil determination apparatus, and overflow enters flask.Flask is placed in electric jacket and is slowly heated
To boiling, and continuous boiling is kept, until the pasta in analyzer no longer changes.1h is placed, opens piston, pasta is made to decline and 0 quarter
Degree is concordant, collects volatile oil and is diluted with 500 μ L chromatographies pure ethyl acetates, is put into 5mL liquid phase bottles and seals in case GC/MS points
Analysis.
Spiderflower volatile substance ingredient is analyzed using GC/MS.It may be referred to document：Wang get Dao, Zhu Yu, Liu Hongzhang wait water
Vapor method extracts casting skin and analyzes [J] Exploitation of Agriculture in Heilongjiang science by loose Pine Needle Essential Oil and GC/MS, and 2013,3：96-97.
GC conditions：Using BD-5MS fused-silica capillary columns (30m × 0.25mm × 0.25 μm)；Injector temperature is
250 DEG C, ECD detector temperatures be 250 DEG C；Carrier gas is high-purity helium, purity>99.999%, flow velocity 1mL/min；Column temperature originates
For 45 DEG C, 5min is kept, 210 DEG C are risen to 4 DEG C/min, keeps 3min, then 230 DEG C are risen to 15 DEG C/min, Splitless injecting samples,
1 μ L of sample size.MS conditions：Ion source EI, 230 DEG C, electron energy 60eV of ion source temperature, 35.0 μ A of emission current, quadrupole rod
165 DEG C of temperature, 275 DEG C of converting interface temperature, electron multiplier voltage 350V, mass scan range 45-500amu.Utilize N
The ingredient that the analysis experiment of 05 standard mass spectral databases of IST obtains.
Spiderflower volatile materials composition analysis result：
21 kinds of compounds are isolated using GC/MS.It is retrieved using N IST05 standards mass spectral database-computer interconnection, reflected altogether
Define 21 kinds of compounds (Fig. 3 and table 1).21 kinds of substances include 13 kinds of heterocycles, and a kind of alcohol, a kind of color is dilute, a kind of ester, a kind of acid and
6 kinds of long chain alkanes.What content was most in the substance detected is paeonol (22.60%), is secondly 1, Isosorbide-5-Nitrae a- trimethyls-
3,4,4a, 5,6,7- hexahydro -2- (1H)-naphthalenone (12.56%), 2,5,5,8a- tetramethyls -3,4,4a, 5,6,8a- hexahydro -2H-
Chromene (8.00%), cis 9,12,15-oc-tadecatrienoic acid methyl esters (6.78%), resin acid (5.04%), linalool (3.74%), 3-
Ethyl -5- (ethyl-butyl)-octadecane (3.46%) plants ketone (3.29%), and tetradecyl oxirane (3.19%), 9- is
Base-heptadecane (3.17%), 35 carbon -17- alkene (3.16%), lignocerane (3.13%), 7- hydroxyls-tetramethyl -7,8-
Dihydro cycloheptyl and pyrans [d, e] naphthalene (3.10%), 7,12a- dimethyl -1,2,3,4,4a, 11,12,12a- is octahydro in the wrong
(2.93%), heptadecane (2.89%), ethylcyclopentenolone (2.69%), tea perfume spirane (2.51%), octacosane
(2.09%), 8- methyl -5 (4- pentenyls) octahydro indolizine (1.95%), 2,6- dimethyl -1- cyclohexenes-acetic acid fat
(1.92%), 4- hydroxy-betas-irisone (1.81%).
1 spiderflower volatile materials ingredient of table
3 spiderflower volatile materials of embodiment is to cucumber fusarium axysporum Antibacterial Activity
GC/MS measures the 8 kinds of substances obtained after spiderflower volatile materials, it is measured to Huang using mycelial growth rate method
The bacteriostatic activity of cucurbit wilt bacterium.
The cucumber fusarium axysporum of 7d will be cultivated on PSA culture mediums, with the card punch of a diameter of 0.85cm in colony edge
Punching cuts bacteria cake, is inoculated in the 9.0cm culture dishes center of PSA, is covered in culture dish and be separately added into the above-mentioned 8 kinds of objects of 0.3g
Matter (analysis is pure), is sealed with sealed membrane, is placed in dark culturing in 20 DEG C of incubators.In this experimental basis, according to tea perfume spirane,
Ratio shared by volatile materials of the 3 kinds of substances of paeonol and linalool in GC/MS experiment acquisitions carries out stifling experimental design, per ware
Middle tea perfume spirane, paeonol and linalool content are respectively：Tea perfume spirane 0g, 0.0075g, 0.0151g, 0.0226g,
0.0301g；Paeonol 0g, 0.0678g, 0.1356g, 0.2034g, 0.2712g；Linalool 0g, 0.0112g, 0.0224g,
0.0337g, 0.0449g, test method are same as above.
Experimental result as shown in figure 4, Fig. 4 for 8 kinds of spiderflower volatile materials 0.3g/ wares to the antibacterial of cucumber fusarium axysporum
Effect, A are blank control；B is tetradecyl oxirane；C is lignocerane；D is heptadecane；E is octacosane；F is second
Cyclopentene alcohol ketone；G is tea perfume spirane；H is linalool；I is paeonol.As can be seen from Fig. 4, tea perfume spirane, linalool, tree peony
Phenol has cucumber fusarium axysporum an inhibitory action, and tetradecyl oxirane, lignocerane, heptadecane, octacosane, ethyl ring
Amylene alcohol ketone is without bacteriostasis.
The bacteriostasis of various dose tea perfume spirane, linalool and paeonol to cucumber fusarium axysporum is further studied, as a result
See Fig. 5 and Fig. 6.In Fig. 5, A is fungistatic effect of the tea perfume spirane to cucumber fusarium axysporum.A1 is blank control；A2 is 0.0075g/
Ware；A3 is 0.0151g/ wares；A4 is 0.0226g/ wares；A5 is 0.0301g/ wares.B is antibacterial effect of the paeonol to cucumber fusarium axysporum
Fruit.B1 is blank control；B2 is 0.0678g/ wares；B3 is 0.1356g/ wares；B4 is 0.2034g/ wares；B5 is 0.2712g/ wares.C
It is linalool to the fungistatic effect of cucumber fusarium axysporum.C1 is blank control；C2 is 0.0112g/ wares；C3 is 0.0224g/ wares；C4
For 0.0337g/ wares；C5 is 0.0449g/ wares.In Fig. 6, the dosage of tea perfume spirane be respectively per ware 0g, 0.0075g, 0.0151g,
0.0226g、0.0301g；The dosage of paeonol is respectively 0g, 0.0678g, 0.1356g, 0.2034g, 0.2712g.Linalool
Dosage is respectively 0g, 0.0112g, 0.0224g, 0.0337g, 0.0449g.
It can be seen that by Fig. 5 and Fig. 6 with the increase of 3 kinds of dosages of substance in every ware, bacteriostasis rate is consequently increased.Tea
When Neptunea cumingi alkane amount is per ware 0.0075g, 0.0151g, 0.0226g, 0.0301g bacteriostasis rate be respectively 66.0%, 83.7%,
89.2% and 91.0%；Bacteriostasis rate is respectively when paeonol amount is 0.0678g, 0.1356g, 0.2034g and 0.2712g
79.7%th, 87.1%, 90.8% and 92.6%；Linalool amount is antibacterial when being 0.0112g, 0.0224g, 0.0337g, 0.0449g
Rate is respectively 92.0%, 97.9%, 95.8% and 98.4%.
Biofumigation is to utilize from what the organic matters such as Cruciferae or composite family generated in decomposition to there is killing livestock property to volatilize
Property gas, inhibit or kill soil in harmful organism method.Crucifer, particularly brassica plant have life
Object fumigation action.Soil-fumigating is carried out using vegetable materials such as leaf mustard, wild cabbage and turnips, a variety of soil-borne plant pathogens of equity are all
It is inhibited.Containing substantial amounts of thio gluconic acid fat, the substance and without very high activity in the platymiscium tissue, but
It can be hydrolyzed in putrefaction process by the myrosin that itself is generated and form volatility and the very strong isothiocyanic acid lipid of killing livestock property
Substance, so as to reduce harmful microbe quantity in soil.
Spiderflower is the one of Capparales Capparidaceae spiderflower category, biennial herb plant, is referred to as purple dragon, swallowtail butterfly
Grass, Western cabbage rolls, spider flower.Spiderflower belongs to Capparales with Cruciferae brassica plant, and whether spiderflower has
The Biofumigation effect of similar brassica plant there is no research to report.Present invention firstly provides spiderflower cauline leaf crushed material to Huang
The inhibitory action of cucurbit wilt bacterium, using GC/MS have detected spiderflower volatile materials active ingredient and each ingredient it is antibacterial
Activity.The result shows that 0.1g/ wares, 0.2g/ wares, the spiderflower crushed material of 4 dosage of 0.3g/ wares and 0.4g/ wares wither to cucumber
Germ has inhibitory action, and wherein 0.3g and 0.4g bacteriostasis rates are up to 100%.GC/MS identifies spiderflower volatile materials 21
Kind, wherein paeonol, linalool and tea perfume spirane have cucumber fusarium axysporum good inhibiting effect, and bacteriostasis rate is respectively
92.6%th, 98.4% and 91.0%.Spiderflower has fumigation action and paeonol, linalool and tea perfume to cucumber fusarium axysporum
Spirane still belongs to the first time discovery for its main antipathogenic composition.
There is no the isosulfocyanate objects of similar brassica plant in the ingredient that spiderflower volatile materials GC/MS is measured
Matter illustrates spiderflower antifungal mechanism and same purpose Brassica genus and differs.What content was most in spiderflower volatile ingredient is tree peony
Phenol, paeonol also known as Paeonol, peonol are the effective extracts of Chinese medicine tree peony.Paeonol has in medical domain widely should
With having the effects that analgesia, calmness, hypnosis, antibacterial, anti-inflammatory, anti-oxidant, blood pressure lowering.It is fragrant in addition to paeonol in this result of study
Camphor tree alcohol and tea perfume spirane also have cucumber fusarium axysporum preferable fumigation action, illustrate that spiderflower fumigates cucumber fusarium axysporum
Effect may be the coefficient result of many kinds of substance.This finds further should in terms of Biofumigation for spiderflower from now on
With being of great significance.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modifications, equivalent replacements and improvements are made should all be included in the protection scope of the present invention.
Claims (10)
1. application of the spiderflower in the activity for inhibiting plant soil-borne diseases pathogenic bacteria.
2. application of the spiderflower in the activity for inhibiting cucumber fusarium axysporum.
3. application according to claim 2, which is characterized in that spiderflower contains paeonol, linalool and tea perfume spirane one
Kind or several mixing.
4. application of the spiderflower in the Biofumigation agent for preventing cucumber fusarium axysporum is prepared.
5. application of the spiderflower volatile ingredient extract in the activity for inhibiting cucumber fusarium axysporum, which is characterized in that spiderflower
Volatile ingredient extract includes the mixing of paeonol, linalool and tea perfume spirane one or more.
6. the application of paeonol, aromatic alcohol or tea perfume spirane in the activity for inhibiting cucumber fusarium axysporum.
A kind of 7. method for the activity for inhibiting cucumber fusarium axysporum using spiderflower, which is characterized in that comprise the following steps：It will be liquor-saturated
Butterfly scape leaf carries out Biofumigation after crushing to cucumber fusarium axysporum.
8. inhibit the method for the activity of cucumber fusarium axysporum using spiderflower according to claim 7, which is characterized in that acquisition
The spiderflower cauline leaf of growth 120-150 days.
A kind of 9. method for inhibiting the activity of cucumber fusarium axysporum using spiderflower volatile ingredient extract, which is characterized in that
Comprise the following steps：Spiderflower volatile ingredient extract, the spiderflower volatile ingredient extract are obtained using steam distillation
Mixing containing paeonol, linalool and tea perfume spirane one or more, by spiderflower volatile ingredient to cucumber fusarium axysporum into
Row Biofumigation.
10. a kind of method for being inhibited the activity of cucumber fusarium axysporum using paeonol, aromatic alcohol or tea perfume spirane, feature are existed
In comprising the following steps：Paeonol, aromatic alcohol or tea perfume spirane are subjected to Biofumigation to cucumber fusarium axysporum.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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Non-Patent Citations (5)
| Title |
|---|
| ANA P. 等: "Antimicrobial Activityand Phytochemical Analysis of Organic Extracts from Cleome spinosa Jaqc.", 《FRONTIERS IN MICROBIOLOGY》 * |
| MCNEIL 等: "Chemical Composition and Antimicrobial Activity of the Essential Oils from Cleome spinosa", 《NATURAL PRODUCT COMMUNICATIONS》 * |
| 于曙光: "丹皮酚、丹皮酚衍生物及其结构类似物的生物活性研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
| 周璇 等: "抑制茄子黄萎菌的植物提取物的筛选", 《生态杂志学》 * |
| 苗建强: "五种挥发性化合物对土传病原真菌及线虫的生物活性", 《植物保护学报》 * |
Cited By (1)
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|---|---|---|---|---|
| CN113180042A (en) * | 2021-04-26 | 2021-07-30 | 江苏省中国科学院植物研究所 | Application of paeonol as agricultural bactericide |
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