CN105410043B - Castor bean meal crude extract is inhibiting the application in pathogenic pythium spp - Google Patents
Castor bean meal crude extract is inhibiting the application in pathogenic pythium spp Download PDFInfo
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- CN105410043B CN105410043B CN201510994198.4A CN201510994198A CN105410043B CN 105410043 B CN105410043 B CN 105410043B CN 201510994198 A CN201510994198 A CN 201510994198A CN 105410043 B CN105410043 B CN 105410043B
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- castor bean
- bean meal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
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- General Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of castor bean meal crude extracts to inhibit the application in pathogenic pythium spp, and the pathogenic pythium spp includes Pythium ultimumPythium ultimum, melon and fruit corruption it is mouldPythium aphanidermatumIt is mould with strong male corruptionPythium arrhenomanes.The preparation of castor bean meal crude extract, including：The a certain amount of castor bean meal powder for smashing it through 80~120 meshes is weighed, distilled water is added, refluxing extraction filters while hot and collect filtrate, and the dregs of a decoction are continued to employ；Distilled water is added in the dregs of a decoction, continues refluxing extraction, filters while hot and collect filtrate；Merge filtrate twice, after evaporation and concentration, obtain brown liquid to get.The present invention opens up new approach to the utilization of castor-oil plant industrial waste not just for providing a kind of excellent novel germicide in production.
Description
Technical field
The present invention relates to plant protection art, specifically a kind of castor bean meal crude extract is inhibiting pathogenic pythium spp
In application.
Background technique
Pythium spp is subordinate to Zao Wu circle Oomycete Peronosporales pythiaceae pythium, is universal soil fungi, saprophytic
It is very capable, root and the seedling of 150 various vegetables fruit crops can be infected, causes to damping off, root-rot, rhizome are rotten and seed is rotten
The multiple diseases such as rotten lose agricultural production serious.Wherein, mould by melon and fruit corruption（Pythium aphanidermatum）It is caused
Samping off is a kind of plant seedlings phase such as melon dish, flowers, medicinal plant, tobacco and beans, cotton, corn, sorghum world wide
Interior destructive disease.Having usually after emergence, basal part of stem place near the ground generates water stain shape dead color spot, then extended around stem, by
Gradually hanging, it is thin-line-shaped to shorten into, rolls over down seedling overground part because losing enabling capabilities, is commonly called as disease of seizing by the throat.Disease portion or soil when humidity is big
Table can generate white cotton floccule, i.e. germ mycelia, sporangiophore and sporangium.Under conditions of benign climate, in a short time can
Outburst, rate of propagation are exceedingly fast, can lead to plant seedlings Large Scale Death under serious conditions, cause to be short of seedling or even ruin kind, to agricultural
Production brings serious economic loss.
The white bacterium ester of current market sales of evil, first frost hymexazol, metalaxyl-mn-zn etc. belong to toxic pesticides（Low toxicity）.
Summary of the invention
The purpose of the present invention is to provide a kind of safe and easily-degradable, noresidue, free of contamination castor bean meal crude extracts to exist
Inhibit the application in pathogenic pythium spp.
To achieve the above object, the present invention provides the following technical solutions：
Castor bean meal crude extract is inhibiting the application in pathogenic pythium spp.
As a further solution of the present invention：The pathogenic pythium spp includes Pythium ultimumPythium ultimum, melon and fruit corruption it is mouldPythium aphanidermatumIt is mould with strong male corruptionPythium arrhenomanes。
As a further solution of the present invention：The preparation of the castor bean meal crude extract, includes the following steps：It weighs
The a certain amount of castor bean meal powder for smashing it through 80~120 meshes, according to castor bean meal powder：The mass volume ratio of distilled water is 1g：8
Distilled water is added in the ratio of~10ml, and 1.5~2.5h of refluxing extraction filters while hot and collect filtrate, and the dregs of a decoction are continued to employ；According still further to
Castor bean meal powder：The mass volume ratio of distilled water is 1g：Distilled water is added in the dregs of a decoction in the ratio of 8~10ml, continues reflux and mentions
1.5~2.5h is taken, filters while hot and collects filtrate；Merge filtrate twice, after evaporation and concentration, obtain brown liquid to get.
As a further solution of the present invention：The dry matter dosage of the castor bean meal crude extract be greater than 0wt.%,
In numberical range no more than 20wt.%.
As a further solution of the present invention：The dry matter dosage of the castor bean meal crude extract is 5~15wt.%.
As a further solution of the present invention：The dry matter dosage of the castor bean meal crude extract is 5wt.%.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention provides castor bean meal crude extracts to inhibit the application in pathogenic pythium spp.Due to the extract
It is natural materials, to Crop securify, is easy to degrade, it is noresidue, pollution-free.Therefore, the present invention in production not just for providing
A kind of excellent novel germicide, and new approach is opened up to the utilization of castor-oil plant industrial waste.
Detailed description of the invention
Fig. 1 is Pythium ultimum（Pythium ultimum）Morphological feature（Wherein, 1：Mycelia expands body；2：Oogonium,
Spermary and egg spore）；
Fig. 2 is that melon and fruit corruption is mould（Pythium aphanidermatum）Morphological feature（Wherein, 1：Sporangium and vesica；
2：Oogonium, spermary and egg spore）；
Fig. 3 is strong male rotten mould（Pythium arrhenomanes）Morphological feature（Wherein, 1：Sporangium；2：Oogonium,
Spermary and egg spore）；
Fig. 4 is castor bean meal crude extract to Pythium ultimum（Pythium ultimum）Growth inhibition result figure；Wherein
The left-to-right dry matter dosage of castor bean meal crude extract is successively 0wt.%, 15wt.%, 10wt.%, 5wt.%；
Fig. 5 is that castor bean meal crude extract is mould to melon and fruit corruption（Pythium aphanidermatum）Growth inhibition result
Figure；Wherein the dry matter dosage of castor bean meal crude extract is successively from left to right 0wt.%, 15wt.%, 10wt.%；
Fig. 6 is castor bean meal crude extract to strong male rotten mould（Pythium arrhenomanes）Growth inhibition result
Figure；Wherein the dry matter dosage of castor bean meal crude extract is successively from left to right 0wt.%, 15wt.%, 10wt.%.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Please refer to Fig. 1~4, in the embodiment of the present invention, castor bean meal crude extract is in inhibiting pathogenic pythium spp
Using, including following steps：
One, the separation and identification of pathogenic pythium spp
Acquire the plant sample with typical rotten mould Disease symptoms.The strong intersection material of disease is chosen, 2 are rinsed with tap water~
3 times, then the fritter for being cut into 5mm or so, aqua sterilisa rinse 3 times, and rotten mould selectivity is put into after blotting surface moisture with aseptic filter paper
Culture medium（NARM）25 DEG C of dark cultures on plate.Picking mycelia tip is moved and is carried out on new NARM culture medium after growing bacterium colony
Purifying, number save to be identified.Strain inoculated will be purified on CMA plate, 25 DEG C of 2~3d of dark culture, with punch in bacterium colony
Edge, which is beaten, takes mycelia block, is transplanted to new CMA plate center, and the lawn blade of grass for cutting 5mm folds up after sterilizing in mycelia block
Around, 25 DEG C of dark cultures.Blade of grass is put into 10ml sterilizing pond water after bacterium colony covering blade of grass to be grown（Pond water：Distillation
The volume ratio of water is=1：2）In 25 DEG C of dark cultures, observed after induced synthesis brood body under the microscope and acquire image.It obtains
3 kinds of corruption are mould：Pythium ultimum（Pythium ultimum）, melon and fruit corruption it is mould（Pythium aphanidermatum）It is mould with strong male corruption
（Pythium arrhenomanes）Morphological feature difference it is as shown in Figure 1,2 and 3.
Two, the preparation of castor bean meal crude extract
The castor bean meal powder 100g for smashing it through 100 meshes is weighed in 1L single necked round bottom flask, 900ml distillation is added
Water, refluxing extraction 2h, filters while hot and collects filtrate.900ml distilled water is added in the dregs of a decoction, continues refluxing extraction 2h, takes advantage of
Heat filtering simultaneously collects filtrate.Merge 2 extracts, obtains brown liquid after evaporation and concentration.
Three, toxicity test
Toxicity test, which uses, inhibits bacterium colony growing method.Castor bean meal crude extract is configured to dry matter dosage difference
For the series of concentrations gradient of 20wt.%, 15wt.%, 10wt.%, 5wt.%, 0wt.%.Bacterial strain will be tried altogether respectively on CMA culture medium
After preculture 2d, fungus block is taken in colony edge with the punch of diameter 5mm, and fungus block is inoculated in containing serial drug concentration
It on CMA plate, is cultivated under 25 DEG C of dark conditions, observes bacterium colony growing state, each processing is repeated 3 times, as a result such as Fig. 4~6
It is shown.By figure it can be concluded that：Dry matter dosage is that the castor bean meal crude extract of 5wt.%~10wt.% can completely inhibit 3 kinds
The growth of pythium spp.
Four, conclusion
The castor bean meal crude extract of low concentration has very strong growth inhibition effect to plant pathogenic fungi.Castor bean meal is thick
Extract has very high utility value on the plant disease control caused by pythium spp.When producing pesticide with the substance, production
Simple process, raw material resources are abundant cheap, and cost is greatly lowered.And castor bean meal crude extract is natural extract,
It is easy to degrade in the environment, can solve problem of environmental pollution brought by the pesticide applied in agricultural production at present.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (4)
1. castor bean meal crude extract is inhibiting the application in pathogenic pythium spp；The pathogenic pythium spp includes eventually
It is extremely rotten mouldPythium ultimum, melon and fruit corruption it is mouldPythium aphanidermatumIt is mould with strong male corruptionPythium arrhenomanes；The preparation of the castor bean meal crude extract, includes the following steps：Weigh it is a certain amount of smash it through 80~
The castor bean meal powder of 120 meshes, according to castor bean meal powder：The mass volume ratio of distilled water is 1g：The ratio of 8~10ml is added
Distilled water, 1.5~2.5h of refluxing extraction filter while hot and collect filtrate, and the dregs of a decoction are continued to employ；According still further to castor bean meal powder：Distilled water
Mass volume ratio be 1g：The ratio of 8~10ml is added distilled water in the dregs of a decoction, continues 1.5~2.5h of refluxing extraction, while hot
It filters and collects filtrate；Merge filtrate twice, after evaporation and concentration, obtain brown liquid to get.
2. castor bean meal crude extract according to claim 1 is inhibiting the application in pathogenic pythium spp, feature
It is, the dry matter dosage of the castor bean meal crude extract is in greater than 0wt.%, the numberical range no more than 20wt.%.
3. castor bean meal crude extract according to claim 2 is inhibiting the application in pathogenic pythium spp, feature
It is, the dry matter dosage of the castor bean meal crude extract is 5~15wt.%.
4. castor bean meal crude extract according to claim 3 is inhibiting the application in pathogenic pythium spp, feature
It is, the dry matter dosage of the castor bean meal crude extract is 5wt.%.
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| CN201510994198.4A CN105410043B (en) | 2015-12-24 | 2015-12-24 | Castor bean meal crude extract is inhibiting the application in pathogenic pythium spp |
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| CN105410043B true CN105410043B (en) | 2018-11-30 |
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| CN108967454A (en) * | 2018-09-21 | 2018-12-11 | 北京弘纳绿源科技发展有限公司 | A kind of plant source extracts crop bactericide and its preparation process |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076682A (en) * | 1993-04-30 | 1993-09-29 | 沈玉水 | Multi-effect compound fertilizer for fruit tree, flower and plant |
| JP2001114620A (en) * | 1999-10-08 | 2001-04-24 | Osaka Organic Chem Ind Ltd | Composition for controlling strawberry disease and pest |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101079491B1 (en) * | 2008-12-17 | 2011-11-02 | 고려바이오주식회사 | Biological germicide for preventing fruit tree disease |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076682A (en) * | 1993-04-30 | 1993-09-29 | 沈玉水 | Multi-effect compound fertilizer for fruit tree, flower and plant |
| JP2001114620A (en) * | 1999-10-08 | 2001-04-24 | Osaka Organic Chem Ind Ltd | Composition for controlling strawberry disease and pest |
Non-Patent Citations (1)
| Title |
|---|
| 蓖麻碱的提取与应用研究进展;穆莎茉莉 等;《内蒙古民族大学学报（自然科学版）》;20120731;第27卷(第4期);第463-466页 * |
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