CN101555456A - Fusarium philoceroides - Google Patents
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- CN101555456A CN101555456A CNA2008102372094A CN200810237209A CN101555456A CN 101555456 A CN101555456 A CN 101555456A CN A2008102372094 A CNA2008102372094 A CN A2008102372094A CN 200810237209 A CN200810237209 A CN 200810237209A CN 101555456 A CN101555456 A CN 101555456A
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Abstract
Fusarium philoceroides is obtained by being separated from natural environment. The fusarium philoceroides is easy to generate spores under conventional culture conditions, the optimal culture medium is potato sucrose agar (PSA), the optimal temperature is 25 DEG C, and the optimal pH value is 7.2; and the optimal condition of pathogen-infecting pathopoiesia comprises that the inoculation concentration is more than 10<5>/m, moisture is kept for 24 hours after inoculating, and the temperature is kept at 23-28 DEG C. The thalli preparation and toxic fluid have strong pathogenic herbicidal effect on malignant weed hollow amaranth; environmental-friendly fungi herbicide can be produced through fermentation thalli or toxin biomimetic synthesis technology; and the fusarium philoceroides has potential commercial development and application values. Pathogenic crude toxin can be obtained from fungi culture fluid through extraction of ethyl acetate and adsorption of active carbon. Thalli suspension and toxic fluid of the fusarium philoceroides have no adverse effects on 18 important crops such as rice and the like.
Description
Technical field
The present invention relates to biological control and the microorganism field of weeds, particularly relate to a kind of fusarium philoceroides (Fusarium philoceroides).
Background technology
Root of Alligator Alternanthera (Alternanthera philoxeroides); have another name called alternanthera philoxeroides or revolution grass; originate in Brazil; be one of the world's ten big evil grass; also be one of 16 kinds of species of first exotic invasive of drafting in January, 2003 of the state guarantee general bureau and the Chinese Academy of Sciences; extensively take place to distribute and harm is caused disaster at more than 20 provinces of China; endanger various floods and droughts farm crop (comprising grain, vegetables, fruit, tobacco, cotton, sugared material, flowers and medicinal material crop etc.), cause serious output and financial loss.According to surveying and determination, the alternanthera philoxeroides production loss that several crops such as paddy rice, wheat, corn and sweet potato cause of causing harm in the time of infertility reaches 45%, 36%, 19% and 63% (Tan Wanzhong, 1994) respectively.Its aquatic type growth and breeding in aquatic environments such as fish pond is rapid, reduces oxygen in water content greatly, and polluted water again after the corruption has a strong impact on hydrobiological growths such as fish, shrimp.Raised growth in river course and irrigation canals and ditches can stop up water channel, and the restriction flow velocity increases deposition, and Water transportation and field irrigation are caused totally unfavorable influence (Li Huaiming, 1994).In the roadside, growths such as public greenery patches, Residential areas destroy the attractive in appearance of environment, and might grow some insects such as mosquitos and flies, harm humans health (Lin Jincheng etc., 2003).Because the adaptive faculty of alternanthera philoxeroides is very strong, therefore might invades wetland etc., thereby squeeze the growth of other plant in addition, make group's species develop, destroy the species diversity (Yu Liuqing etc., 1998) in the ecotope towards the simplification direction.
In China, the measure of preventing and kill off alternanthera philoxeroides mainly is manually to pull out and use chemical herbicides such as glyphosate, but artificial weeding is time-consuming and be difficult to reach the ideal effect, and uses chemical herbicide can cause environmental pollution and residual (Lou Yuan comes etc., 2002) too much.So the biological control of weeds has been subjected to the attention of height in recent years.China Agricultural Sciences Academy Biological Control Institute introduced bent line chrysomelid (Agasicles hygrophila) from Fla. in 1987, the feeding habits of this herbivorous insect are more single-minded, draw may not can after putting to staple crops constitute a threat to (Wu Zhenquan etc., 1994).Except bent line was chrysomelid, China scientific research personnel also was devoted to other natural enemy investigation of alternanthera philoxeroides.Crown canopy human relations (1990) and Cao Huaguo (1997) etc. have found multiple insect of getting the food alternanthera philoxeroides.
China also gets along with aspect the alternanthera philoxeroides utilizing microorganism to prevent and kill off.Tan Wanzhong etc. have found a kind of anthrax bacteria on alternanthera philoxeroides, be dish long spore thorn dish spore (Colletotrichum gloeophorospora) through the preliminary evaluation cause of disease, and the experiment proved that alternanthera philoxeroides is had stronger restraining effect (Tan Wanzhong, 1993).Xiang Meimei etc. (1999) are separated to has inhibiting Alternanthera sessilis (L.) DC. vacation every sickle-like bacteria (Nimbya alternantherae) to alternanthera philoxeroides, and its growth and product spore condition are explored.Plant pathogenic microorganisms tends to produce nosotoxin (Strobel, 1992) in infecting the process that host plant makes it to fall ill, the exploitation that is found to be novel herbicide of these toxin provides enough foundations.These natural physiologically active substances have efficiently, low toxicity, safe, free from environmental pollution, noresidue and compare easy storage with microbial herbicide, process, use, its effect does not rely on envrionment conditions, advantages such as prediction easily.
In recent years, control going deep into of grass research along with the development of biochemical technology, Protocols in Molecular Biology and biotechnology and with bacterium, weeds scientist and chemistry of pesticide men pay close attention to the weeding activity of microbial metabolites more, for seeking new natural compounds weedicide or providing important evidence for bionical synthesizing new weedicide.At present, the various countries scholar studies culture condition, sphere of action, separation and purification, weeding activity, component and the structure etc. of some weeds pathogenic fungi toxin.Utilize the plant pathogenic fungi toxin, particularly some host selection toxin is controlled weed growth, has demonstrated bright development prospect, also is the important channel of development of new weedicide.
However, alternanthera philoxeroides in the generation area of China still in continuous expansion, endanger and the financial loss that causes more and more serious.Therefore, seek this nauseating weeds are had the pathogenic micro-organism of pathogenic by force and biological and ecological methods to prevent plant disease, pests, and erosion control action kou, further develop noresidue, campelyco free from environmental pollution thus, to not only have the important commercial development and application values, and can control causing harm of root of Alligator Alternanthera safely and effectively, avoid the tremendous economic loss that causes thus.
Summary of the invention
The purpose of this invention is to provide a kind of careless fungi that kills that root of Alligator Alternanthera is had very strong pathogenic effects and control effect.According to morphological feature with based on the molecular biotechnology of " translation elongation factor " gene (TEF) it is accredited as a sickle-like bacteria (Fusarium) novel species, determined its growth product spore and infected morbific optimum condition by test determination, discover that it makes the dead pathogenesis of weeds plant morbidity by producing toxin, has analyzed host's specialization of germ and toxin thereof.
The present invention seeks to be achieved through the following technical solutions:
Fusarium philoceroides bacterial strain of the present invention comes by the following steps separation and purification:
Repeatedly gather root of Alligator Alternanthera natural occurrence plant from different places season in Chongqing and Sichuan, adopt potato sucrose nutrient agar (PSA), obtain dissimilar fungies through indoor tissue culture and purifying, demonstrate,prove the disease method with Ke He Shi and determine wherein pathogenic bacterium, at last through pathogenic test relatively screen acquisition to root of Alligator Alternanthera have the careless pathogenic fungi of killing of very strong pathogenic effects (
The FPT001 bacterial strain).Extract the genomic dna of bacterial strain, use primer ef1[5 '-ATGGGTAAGGA (A/G) GACAAGAC-3 ')] and ef2[5 '-GGA (G/A) GTACCAGT (G/C) ATCATGTT-3 '] the translation elongation factor gene order that pcr amplification obtains this fungi done, its length is 670bp, is EU668362 in the accession number of Genbank.By " sickle-like bacteria evaluation " (Fusarium-ID) software evaluation that Blast analyses and compares and U.S. sickle-like bacteria research centre is set up, this bacterium is Fusarium one novel species.
Fusarium philoceroides bacterial strain of the present invention on July 3rd, 2008 be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (Datun Road, Chaoyang District, Beijing City, postcode: 100101), preserving number: CGMCC NO.2570.
It below is the detailed description of fusarium philoceroides
FPT001 kills the morphological specificity and the molecular engineering of careless bacterial strain and identifies: on potato sucrose agar (PSA) substratum, colony growth speed is very fast, cultivate to be measured as 30～40mm in 4 days, and initial stage white, the generation owing to conidium group later on becomes rose pink; The bacterium colony circle, the aerial hyphae on surface is sparse, and white is curled; Conidiogenous cell bottle stalk shape, on it give birth to single macroconidium; Macroconidium produces in a large number, is sickle shaped, and is more straight or little curved, divides 3～5 every (rare 6 every), and apical cell is the beak shape, spore size 40.6～55.2 * 5.5～6.1 μ m; Basically do not produce sporule; The chlamydospore ball-type, colourless, smooth surface, diameter 9.1～12.0 μ m result between the cell of mycelia or macroconidium in a large number.According to these morphological specificitys, in conjunction with TEF gene identification result, this fungi named to be the root of Alligator Alternanthera sickle-like bacteria, the Latin formal name used at school is Fusarium philoxeroides.
This is a novel species on classification of fungi is learned, and it is according to mainly containing: 1) consult the relevant classification document of existing Fusarium and other bibliographys (old its, 1988, Fusarium. agricultural is published; Booth C, 1971, The genus fusarium; Http:// www.doctorfungus.org/), the morphological specificity of this sickle-like bacteria and other kinds have notable difference; This point is through the checkings such as doctor Gerser in USDA " sickle-like bacteria research centre "; 2) analyze by Blast and with the special-purpose Fusarium-ID software analysis of Fusarium classification, the TEF gene similarity of the reaping hook bacterial classification that TEF gene order and all of this sickle-like bacteria have been put down in writing is less than (similarity can be defined as different kinds less than 98% in the fungi) more than 96%.
The best of fusarium philoceroides is cultivated and the inoculation infection condition: this fungal colony is grown and produced conidial optimum medium is potato sucrose agar (PSA), 25 ℃ of optimum temperutures, Optimum pH is pH7.2, under these conditions, colony growth is fast, produces macroconidium and chlamydospore in a large number; Illumination is produced spore to fungal growth does not have obvious influence.The morbific top condition of infection process be inoculum density at the 105/ml order of magnitude or higher, preserve moisture after the inoculation and half-light 24 hours, and keep 23～28 ℃ of temperature in the whole phase of infecting.
Pathogenic and the specialization of fusarium philoceroides: pathogenic effects and growth inhibitory effect that the root of Alligator Alternanthera sickle-like bacteria is good to the root of Alligator Alternanthera tool.Mainly infect plant leaf and stem stalk.Behind the direct spray inoculation of the conidial suspension of indoor germ, can fall ill in 3 days, diseased plant rate reaches 100%, and the plant top begins to wilt blade dehydration yellow; Whole plant wilting death after 9 days, its control effect and glyphosate to root of Alligator Alternanthera is suitable.Effect manifests delay slightly in inoculation back, field, the whole yellows in plant top in the 5th day, and it is dead to wilt after 2 weeks.
Use high density conidial suspension (108/ml) inoculation test result to show under optimum conditions, this fungi is all not pathogenic at interior 18 kinds of water of 6 sections, Dry crop to important crops such as paddy rice, wheat, corn, pea, rape, capsicum, tomato, cottons, do not influence their seed germination and plant strain growth yet, this explanation fusarium philoceroides is the obligate pathogenic bacterium of root of Alligator Alternanthera, if be used to develop the root of Alligator Alternanthera weedicide, can guarantee the safety of other plant in the habitat.
The thick toxin of fusarium philoceroides: with the nutrient solution of fungi, process ethyl acetate or active carbon adsorption extraction, rotatory evaporator vacuum-evaporation obtain the thick toxin that causes a disease.Flood respectively behind root of Alligator Alternanthera blade and the stem stalk with the water liquid of this toxin and promptly to show signs of toxicity, intra vane yellow in 72 hours in 24 hours.Do not observe signs of toxicity after handling the seed of aforementioned 20 kind of plant and plant with this thick toxin soiutions, illustrate that this toxin also is highly specialized.
Description of drawings
Figure l is the morphological specificity of fusarium philoceroides bacterium colony
Fig. 2 is the morphological specificity of fusarium philoceroides: A is a macroconidium; B is a conidiophore; C is the generation of conidium chlamydospore; D is that mycelia produces chlamydospore
Fig. 3 is the translation elongation factor gene TEF sequence of fusarium philoceroides
Fig. 4 kills careless effect for fusarium philoceroides to the indoor and field of root of Alligator Alternanthera: last figure is the root of Alligator Alternanthera plant death (the right plant is contrast) of all wilting after 9 days of greenhouse internal spraying inoculation conidial suspension, and figure below is field inoculation root of Alligator Alternanthera plant top yellow after 5 days.
Fig. 5 is contrast for kill careless effect: A and the B of root of Alligator Alternanthera toxin, and C and D handle the blade effect with ethyl acetate and the isolating thick toxin of gac.
Advantage of the present invention is: separate the fusarium philoceroides (Fusrium that obtains from natural environment Philoxeroides), be a new species of fungi of finding first and identifying, its conidium inoculum is greatly pernicious to the world ten The alligator alternanthera root or herb of one of weeds (Alternanthera philoxeroides) has very strong pathogenic control action, its secretion Toxin also has the deadly effect of very strong toxicity to this weeds. The microbial inoculum of germ and toxin in important crops and the environment other Plant does not all have harmful effect, shows for the height of alligator alternanthera root or herb specially to change removing activity, can be by fermentation thalline or bionical closing The environment-friendly type fungoid herbicide of one-tenth technology production noresidue has potential business development and using value, simultaneously hollow Also has important development prospect in the biological control practice of amaranth.
Embodiment
Below in conjunction with embodiment the present invention is further described
EXAMPLE l:
Kill separation and purification and the screening of careless fungi
(1) fungi separation and Culture potato sucrose nutrient agar (PSA), its prescription is the fresh potato 200g of peeling, each 20g of sucrose and agar adds tap water to 1000ml.It is well-done with potato to add water earlier, and double gauze filters the back and adds sucrose and agar, replenishes an amount of water to 1000ml, in the triangular flask of packing into after fully mixing evenly, puts into sterilizer sterilization back and places.Heating and melting before using is poured in sterilization culture dish or the test tube and is made flat board or slant medium, uses for fungi separation and Culture or bacterial classification preservation.
(2) separation and Culture and purifying: under natural condition, gather root of Alligator Alternanthera plant sample with typical disease symptom, the clean back of rinsing clip leaf spot lesion and healthy part are had a common boundary organizes little (2mm), put into 5% chlorine bleach liquor's surface sterilization and use the aqua sterilisa rinsing later on 3 times, place the PSA flat board, in 25 ℃ of incubators, cultivate and observation every day, treat to extract the mycelia tip respectively after different bacterium colonies grow in good time, transfer to and continue to cultivate into pure bacterium colony on the new PSA flat board, transfer to again on the PSA test tube slant substratum, and number and indicate bacterial strain, preserve standby.
(3) determine pathogenic bacteria: determine pathogenic bacteria according to the sick rule of Ke He Shi card step.Smear or the inoculation of acupuncture drop for healthy root of Alligator Alternanthera plant leaf with obtaining various fungies after the separation and purification of sick leaf texture, lucifuge was preserved moisture 24 hours, 28 ℃ of growths down, observed incidence.The fungi that causes the morbidity of inoculation blade, performance same symptoms can be defined as pathogenic bacteria.
(4) strong pathogenic bacterium screening: above-mentioned test is determined that all pathogenic bacterias inoculate to the root of Alligator Alternanthera plant with identical method (smearing mist or acupuncture drop), and cultivating the severity of observing disease on the inoculation plant under the identical suitable condition, relatively filter out the bacterial strain that causes that root of Alligator Alternanthera morbidity is serious, screening has obtained fusarium philoceroides thus.
(5) evaluation of pathogenic bacteria: 1) morphological observation and evaluation. on the PSA flat board, cultivate pathogenic bacteria, observe colonial morphology, conidium and chlamydosporic microscopic features in good time; 2) molecular biology identification. according to the technology of Geiser foundation, extract the genomic dna of bacterial strain, use primer ef1[5 '-ATGGGTAAGGA (A/G) GACAAGAC-3 ')] and ef2[5 '-GGA (G/A) GTACCAGT (G/C) ATCATGTT-3 '] the translation elongation factor gene order that pcr amplification obtains this fungi done, check order, and sequence is signed in among the Genbank; This sequence is Blast analyzes, adopt the kind of all existing Fusarium in the Fusarium-ID software to compare simultaneously, determine the classification position of this sickle-like bacteria and the name of kind.
Embodiment 2:
The cultivation of fusarium philoceroides and inoculation condition test
(1) humid test: cultivating on the PDA flat board of 5d with the punch tool cut-off directly is the mycelia piece of the FPT001 bacterial strain of 0.5cm, be inoculated on the PDA substratum, establish 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃ and 40 ℃ of 8 Temperature Treatment respectively, each is handled and repeats 3 times.Place 7 pre-set temperature required illumination boxs to cultivate, and measure the bacterium colony size day by day with vertical cross method, every 24h measures 1 time, continuously measured 5 times, record data.The potential of hydrogen of substratum is about pH7.0.Determine the optimum growth temperature of sickle-like bacteria thus.
(2) potential of hydrogen experiment: the PDA substratum is adopted in experiment, 11 potential of hydrogen is set altogether handles.With the NaOH of 1.0mol/L and HCl solution the pH value of substratum is adjusted to 4.0,5.0,6.0,6.5,7.0,7.5,8.0,9.0,10.0,11.0 and 12.0 (measuring with the electronics pH meter) respectively respectively, each is handled and repeats 5 times; Germ inoculation and growth measuring method are the same.Connect the bacterium processing and be placed on 25 ℃ of cultivations down, every 24h measures a colony diameter, continuously measured 6 times.Determine the optimal ph of sickle-like bacteria growth thus.
(3) screening of kinds of culture medium: the influence of 6 kinds of substratum to the fusarium philoceroides colony growth compared in experiment altogether, except potato sucrose substratum (PSA), other five kinds of substratum are respectively: 1) potato dextrose agar (PDA): water 1000ml, potato 200g, glucose 20g and agar powder 20g; 2) clear water agar medium (WA): water 1000ml and agar powder 20g; 3) Radix Dauci Sativae substratum (CAA): water 1000ml, Radix Dauci Sativae 200g, agar powder 20g; 4) the alternanthera philoxeroides rhizome is fried in shallow oil juice substratum (ARS): water 1000ml, alternanthera philoxeroides rhizome 200g and agar powder 20g; 5) the alternanthera philoxeroides blade is fried in shallow oil juice substratum (ALA): water 1000ml, alternanthera philoxeroides blade 200g and agar powder 20g.When preparation PDA, CAA, ARS and ALA, potato, Radix Dauci Sativae, weeds rhizome or blade with aequum shreds and puts into water well-done (boiling approximately 30 minutes) earlier, and after-filtration is removed residue and be settled to 1000ml, the last chemical substance composition that adds aequum again, stir, contain in the triangular flask standby behind the moist heat sterilization respectively.The pH value of various substratum is about 6.8-7.0,25 ℃ of culture temperature.Determine the optimal medium that germ is cultivated thus.
(4) the germ inoculation condition is optimized: generally influence the shadow that plant pathogenic fungi infects and mainly contain several concentration, temperature, moisture preserving time and illumination.Be provided with 7 several concentration (4.50 * 103/ml among the present invention, 1.25 * 104/ml, 6.26 * 104/ml, 1.62 * 105/ml, 3.20 * 105/ml, 5.28 * 106/ml, 1.37 * 108/ml), and be positioned at 5 temperature between 20～30 ℃ according to optimal growth condition being provided with of aforementioned hollow amaranth biological and ecological methods to prevent plant disease, pests, and erosion sickle-like bacteria, and preserve moisture (%) time 0,12,24,32 and 48h, illumination is set to shine respectively under 1200Lux and the 3000Lux 0,6,12,18 and 24h.Each Temperature Treatment 3 strain root of Alligator Alternanthera, 5～6 blades of every strain.Analyze the suitable inoculation onset condition (inoculum density, temperature, moisture preserving time and illumination) of determining germ thus.
Embodiment 3:
The thick toxin of pathogenic bacteria separates
(1) preparation of germ culturing filtrate: pour 250ml potato sucrose (PS) nutrient solution in the 500ml triangular flask respectively into, the sterilization back is stand-by.Choose and cultivate the bacterium colony that 6-7d grows fine, get the bacterium cake of Φ 6mm size, be inoculated in the triangular flask that 250ml PS nutrient solution is housed, 8 of every bottle graft kinds, static cultivation 15 days under 12h illumination/sky condition in 25 ℃ of incubators with punch tool.On Bechtop, filter with qualitative filter paper (middling speed), can obtain culturing filtrate.Prepare the usefulness of enough germ culturing filtrates thus for the toxin separation and Extraction.
(2) extraction of toxin and activity test: 1) CAM extracts: after culturing filtrate is added 5% activated carbon powder and fully shakes, place in 5～6 ℃ of refrigerators and leave standstill 12h, use the filter paper filtering carbon dust, the activated carbon powder that filtration is obtained is soaked in 40 ℃ of hot methanols of 10 times of volumes then, leave standstill after fully stirring, remove insolubles, concentrate faint yellow dissolve with methanol liquid to tawny, again enriched material is dissolved in the 50mL hot methanol (40 ℃), shift out insolubles once more, supernatant liquor adds the chloroform precipitation of 3 times of volumes, shifts out yellow insolubles, remaining methyl alcohol-chloroform lysate is evaporated to dried, promptly obtains thick toxin.2) organic solvent extraction: culturing filtrate is extracted with ethyl acetate, the culturing filtrate of each volume extracts 3 times with isopyknic ethyl acetate, uses the ethyl acetate of 1/3 volume at every turn, merges organic phase then, through rotary evaporation, can obtain enriched material behind the recovery organic solvent.
Ethyl acetate extraction and active carbon adsorption are obtained thick toxin respectively with using work to root of Alligator Alternanthera blade dip treating after the water dissolution, measure its activity.Two kinds of extracts of result all show very strong toxicity to root of Alligator Alternanthera.
Embodiment 4:
Safety (specialization) the property mensuration of pathogenic bacteria and toxin thereof
(1) for the crop of testing: 18 kinds of important floods and droughts crops having selected 6 sections, comprise paddy rice gramineous, wheat, corn, the tomato of Solanaceae, capsicum and tobacco, cucumber cucurbitaceous and watermelon, the radish of Cruciferae, wild cabbage, rape and Chinese cabbage, the peanut of pulse family, pea, broad bean, soybean and mung bean, and the cotton of Malvaceae.
(2) seed germination test: the healthy seed of selecting aforementioned 18 kinds of crops, (the small-sized seed treatment time is short to use mycelia and spore suspension, the thick toxin soiutions of germ and clear water (contrast) to soak 12～36 hours respectively, the large seed treatment time is long), be placed on the wet filter paper, in 25 ℃ of growth casees, sprout back record seed germination rate.The result shows that the crop seed germination rate of being tested does not all have significant difference with contrast.
(3) crop plant growth test:, be placed on the wet filter paper vernalization in 25 ℃ of growth casees with warm water soaking 12～36h (deciding) according to seed size.Treat to be seeded in the potted plant alms bowl after plumule exposes.Add sandy soil in the alms bowl, add a certain amount of nutritive medium (adding amount of urea, ammonium sulfate and potassiumphosphate in the water) then through washing and hyperthermia drying.After planting grow under greenhouse experiment, use mycelia and spore suspension, the thick toxin soiutions of germ and clear water (contrast) spraying to handle when treating plant length to suitable size respectively, the while serves as the morbidity contrast with processing root of Alligator Alternanthera plant.Handle the pathology situation of back each kind of plant of observed and recorded every day, results plant during by the 15th day, the increment of mensuration different treatment.The result shows, handle back root of Alligator Alternanthera plant with fusarium philoceroides bacterium liquid and toxin solution and after processing, promptly showed tangible disease and toxin symptom respectively in the 3rd day and the 2nd day, and all 18 kinds of crop plants after handling 15 days also without any pathology, do not have significant difference between the increment with growth increment after bacterium liquid and the toxin solution processing and clear water adjoining tree.This illustrates that this germ and toxin thereof be not to having any detrimentally affect for studying thing.
Sequence table
1 tagtcgtcat?cggccacgtc?gactctggca?agtcgaccac?tgtgagtaca?accctcggcg
61 agctgcttgc?ctgcactcgt?caaaccccgc?ctgtgatcca?tggcggggac?ttcagccata
121 tcatgctaac?aactttggat?agaccggtca?cttgatctac?cagtgcggtg?gtatcgacaa
181 gcgaaccatc?gagaagttcg?agaaggttgg?ttatttccct?ccgatcagcg?cccttttgcc
241 cttcgatttg?cgcgtcgaat?cgcttccttc?acgatttcca?acacgccgtt?accccgctcg
301 agcacaaaat?tttgcggtgc?gaccgtaatt?ttttttggtg?gggcatttta?ccccgccact
361 cgagctggga?cacgcaagcc?ctgttccctg?cacacaatca?tcatccgggc?gcgatcatga
421 tacgtgaaac?agttgctaac?caccttgaca?ataggaagcc?gccgagctcg?gtaagggttc
481 cttcaagtac?gcttgggttc?ttgacaagct?caaagccgag?cgtgagcgtg?gtatcaccat
541 cgacattgcc?ctgtggaagt?tcgagactcc?ccgctactat?gtcaccgtta?ttggtatgtt
601 ttcaccatcc?tctctcatgt?gctcgttcta?acaacatgca?gacgctcccg?gccaccgtga
661 cttcatcaag
Claims (1)
1, a kind of fusarium philoceroides (Fusarium philoceroides), it is characterized in that: bacterial strain is FPT001, the preserving number of this bacterial strain is CGMCC NO.2570.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993826A (en) * | 2010-08-23 | 2011-03-30 | 浙江工业大学 | Fusarium sp. BYB2 and application thereof for preparing acacia enzyme by microbial fermentation |
| CN104250620A (en) * | 2014-09-24 | 2014-12-31 | 山东省农业科学院植物保护研究所 | Chlamydospore fusarium strain and application thereof |
| CN105053024A (en) * | 2015-08-06 | 2015-11-18 | 丁德凤 | Microbial herbicide |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
-
2008
- 2008-12-23 CN CNA2008102372094A patent/CN101555456A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993826A (en) * | 2010-08-23 | 2011-03-30 | 浙江工业大学 | Fusarium sp. BYB2 and application thereof for preparing acacia enzyme by microbial fermentation |
| CN101993826B (en) * | 2010-08-23 | 2012-11-14 | 浙江工业大学 | Fusarium sp. BYB2 and application thereof for preparing acacia enzyme by microbial fermentation |
| CN104250620A (en) * | 2014-09-24 | 2014-12-31 | 山东省农业科学院植物保护研究所 | Chlamydospore fusarium strain and application thereof |
| CN105053024A (en) * | 2015-08-06 | 2015-11-18 | 丁德凤 | Microbial herbicide |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
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