CN101508959A - Water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata - Google Patents
Water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata Download PDFInfo
- Publication number
- CN101508959A CN101508959A CNA200810237208XA CN200810237208A CN101508959A CN 101508959 A CN101508959 A CN 101508959A CN A200810237208X A CNA200810237208X A CN A200810237208XA CN 200810237208 A CN200810237208 A CN 200810237208A CN 101508959 A CN101508959 A CN 101508959A
- Authority
- CN
- China
- Prior art keywords
- toxin
- herba eichhorniae
- optimum
- spore
- water hyacinth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A water hyacinth fungal biocontrol agent, Alternaria crassipies, is separated from the natural environment and obtained. Spores are easily produced by the fungus under ultraviolet irradiation, the optimum culture medium for bacterial colony growth is Potato Sucrose Agar (PSA), the optimum temperature is 25 to 30 DEG C, and the optimum pH is 7.0 to 10; and the optimum conditions of fungal infection pathopoiesia are that moisturizing is carried out for 24 hours in dark light after inoculation and the temperature keeps at 28 to 30 DEG C during infection period. The thalli preparation and toxin liquid have very strong effects of pathopoiesia and grass killing on malignant ruderal water hyacinth, thereby being capable of producing environment-friendly mycoherbicide by the technology of biomimetic synthesis for zymogen or toxin and having potential business development using value. Pathopoiesia crude toxin is obtained from fungus culture fluid through extraction with ethyl acetate and reduced pressure distillation. The thalli suspension and the toxin liquid have no adverse influence on 20 kinds of important crops such as rice and the like.
Description
Technical field
The present invention relates to biological control and the microorganism field of weeds, particularly relate to a kind of water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata (Alternaria crassipes).
Background technology
Herba Eichhorniae (Eichhornia crassipes), have another name called water lettuce, originate in South America, being one of the world's ten big evil grass, also is one of most important harmful species of invading China, overflows at 17 provinces of China, its harm is the most serious to be that Zhejiang, Fujian, Guangdong, Yunnan and Tai Wanwu economize, mainly be pollution and the obstruction that causes waters such as rivers lakes and marshes reservoir, hinder the water transportation transportation and disturb the normal operation in power station, cause enormous economic loss every year.
At present, mainly adopt artificial salvaging control and chemical herbicide to prevent and kill off to Herba Eichhorniae both at home and abroad, though certain effect is arranged, the preventive effect of two kinds of measures is all very limited, salvage and only can remove the Herba Eichhorniae plant, stay the weeds group of hill that seed still can be sprouted and very fast formation is new in the water body; And after using chemical herbicide to kill weeds, plant residue can make water body organise and cause environmental pollution, chemicals itself also to exist problem of environmental pollutions such as drug residue and non-target organism safety.Therefore; people have carried out the exploration to the biological control of weeds in recent years; the sixties in 20th century; in phytophage insects such as research and utilization Herba Eichhorniae weevil; many countries have also made a large amount of enquiry based works to the Herba Eichhorniae pathogenic bacteria; and obtained certain progress: the whole world has reported that kind more than 60 infects the pathogenic fungi of Herba Eichhorniae; the Nuo Manni tail spore (Cercospora rodmanii) and the Herba Eichhorniae chain lattice spore (Alternaria eichhorniae) that have screened in countries such as South Africa have stronger restraining effect to Herba Eichhorniae; wherein the Nuo Manni tail spore technology of preventing and kill off Herba Eichhorniae has obtained the international monopoly protection; but this pathogenic bacteria is all quite limited to control effect and the range of application of weeds, yet there are no it at other countries or the regional report that is employed.
China also once had the people that Herba Eichhorniae disease and pathogenic fungi are investigated and studied, the Herba Eichhorniae pathomycete of finding mainly contains Alternaria tenuissima (Alternaria tenuissima), point spore tail spore (Cladosporium oxysporum), A Shi plan dish stey (Pestalotiopsis adusta), Herba Eichhorniae monospore (Monosporium eichhorniae) and thanatephorus cucumeris(frank) donk (Thanatephorus cucumeris) etc., but these fungies are all very limited to the pathogenic effects of Herba Eichhorniae, there is not actual application value, so far yet not about the further research of these germs and the play-by-play of application.
Therefore, seek Herba Eichhorniae is had the pathogenic micro-organism of pathogenic by force and biological and ecological methods to prevent plant disease, pests, and erosion control action kou, further develop noresidue, campelyco free from environmental pollution thus, to not only have the important commercial development and application values, and can control the harm of Herba Eichhorniae safely and effectively, with the tremendous economic loss of avoiding causing thus.
Summary of the invention
The purpose of this invention is to provide and a kind ofly kill careless fungi---Herba Eichhorniae alternaric bacteria strain what Herba Eichhorniae had very strong pathogenic effects and a control effect.According to morphological feature it is decided to be a chain lattice spore novel species, determined its growth product spore and infected morbific optimum condition by test determination, discover that it makes the dead pathogenesis of weeds plant morbidity by producing toxin, has analyzed host's specialization of germ and toxin thereof.
The present invention seeks to be achieved through the following technical solutions:
Herba Eichhorniae chain lattice spore of the present invention kills careless bacterial strain and comes by the following steps separation and purification:
Repeatedly gather Herba Eichhorniae natural occurrence plant in the Chongqing region Various Seasonal, adopt potato sucrose nutrient agar (PSA), obtain dissimilar fungies through indoor tissue culture and purifying, demonstrate,prove the definite pathogenic bacterium wherein of disease method with Ke He Shi, relatively screening acquisition through pathogenic test at last has the careless pathogenic fungi of killing of very strong pathogenic effects (AFT001 bacterial strain) to Herba Eichhorniae.
Herba Eichhorniae alternaric bacteria of the present invention strain on July 3rd, 2008 be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (Datun Road, Chaoyang District, Beijing City, postcode: 100101), preserving number: CGMCC NO.2569.
It below is the detailed description of Herba Eichhorniae chain lattice spore
Herba Eichhorniae chain lattice spore kills the morphological specificity of careless bacterial strain and identifies with classification: colony growth speed is very fast on the PSA substratum, all can reach about 70mm at 25 ℃ of following full exposures and full dark culturing 5d colony diameter.Mycelia is partly buried life, the part hypergene, and aerial hyphae fine hair shape, bacterium colony just fade to the grey black look, back side blackish green for after dark green.Conidia chain is given birth to, and general 7～12 spores are bar-shaped, avette, falls pyriform or near oval, filbert to the moderate brown, spore body 24.7 (12.5～37.5) * 10.1 (7.5～12.5) μ m, the short beak of tool or do not have beak, 0～30 * 2.5-4 μ m.Ripe spore surface tool pustule, 1～3 tabula of tool, 0～4 mediastinum except that time production spore of top, is common in lateral any position and produces short stalk.Conidiophore is upright, and is straight or slightly curved, separate, and not branch or branch, extremely light brown is to filbert, extends along with producing the spore shaft type (sympodial) of making a match, and visible surface is given birth to thin boss, 23.5～70.5 * 3.5～5 μ m under the high power lens.According to these features, with reference to documents and materials (as: Zhang Tianyu, 2003 Chinese fungi will---Alternaria, the Science Presses of relevant fungi and Alternaria evaluation; Http:// www.doctorfungus.org/) identifies, this bacterium is one new member (member) of Alternaria tenuissima group in the Alternaria (Alternaia tenuissimagroup), with its called after Herba Eichhorniae chain lattice spore, the Latin formal name used at school is Alternaria crassipes.This is a novel species on classification of fungi is learned, and it is according to mainly containing: 1) consult relevant classification document and other bibliographys of existing Fusarium, morphological specificity and other kinds of this chain lattice spore have notable difference; This point is through professor's Zhang Tianyu checking of international Alternaria classification of fungi authoritative expert, Shandong Agricultural University; 2) the cause of disease alternaric bacteria of having put down in writing so far on the Herba Eichhorniae plant has 2 kinds, be interlinkage spore (Alternaria alternata) Herba Eichhorniae chain lattice spores (Alternaria eichhornia), isostructural form of their conidium and the difference of this kind chain lattice spore are very big, and the interlinkage spore is a kind of weak bacterial parasite, the disease symptom that it causes is very slight, Herba Eichhorniae chain lattice spore mainly causes a kind of " black spot ", " leaf spot " that symptom and this chain lattice spore cause has essence difference (Charudattan R, 2001.Biological control of water hyacinth by using pathogens, ACIAR Proceedings 102,21-28; Shabana, YM et al, Biological control of water hyacinth by mycoherbicidein Egypt.ACIAR Proceedings 102,53-56).
The best of Herba Eichhorniae chain lattice spore is cultivated and infection condition: this pathomycete is not easy to produce spore under normal illumination and dark condition, and it is very fast to produce spore under UV-irradiation; The colony growth optimum medium is potato sucrose agar (PSA), and optimum temperuture 25-30 ℃, Optimum pH is pH7.0～10; The morbific top condition of infection process is to preserve moisture and half-light 24 hours after the inoculation, and keeps 28～30 ℃ of temperature in the whole phase of infecting.
Pathogenic and the specialization of Herba Eichhorniae chain lattice spore: Herba Eichhorniae chain lattice spore has good causing a disease and the growth-inhibiting effect to Herba Eichhorniae, mainly infect plant leaf, inoculating back 7 days can fall ill, sick leaf rate reaches 100%, the circle that occurs differing in size on the initial stage blade is to irregular scab, extend to petiole later on, the scab tow sides and are given birth to the mould layer of beige, cause big area blade even whole plant withered.Show with high density mycelia fragment and conidial suspension inoculation test result under optimum conditions, this fungi is all not pathogenic at interior 16 kinds of water of 6 sections, Dry crop to important crops such as paddy rice, wheat, corn, pea, rape, capsicum, tomato, cottons, do not influence their seed germination and plant strain growth yet, this explanation Herba Eichhorniae chain lattice spore is the obligate pathogenic bacterium of Herba Eichhorniae, if be used to develop the Herba Eichhorniae weedicide, can guarantee the safety of other plant in the habitat.
The thick toxin of Herba Eichhorniae chain lattice spore:, obtain nosotoxin through ethyl acetate extraction, rotatory evaporator vacuum distilling with the nutrient solution of fungi.Promptly show signs of toxicity in 24 hours behind steep water cucurbit blade and the plant root respectively with the water liquid of this toxin, intra vane yellow in 72 hours, plant wilt (but not moving back green).Do not observe signs of toxicity after handling the seed of aforementioned 20 kind of plant and plant with this thick toxin soiutions, illustrate that this toxin also is highly specialized.
Description of drawings
Fig. 1 is the morphological specificity figure of Herba Eichhorniae chain lattice spore:
1 is the bacterium colony initial stage on the PSA flat board, and 2 is the bacterium colony later stage on the PSA flat board, and 3 is conidia chain, and 4 is conidium, and 5 is conidium type figure, and 6 is conidiophore
Fig. 2 is Herba Eichhorniae chain lattice spore and toxin thereof the pathogenic effects figure to Herba Eichhorniae:
7 is the Herba Eichhorniae plant of handling with thalline the 15th day, and 8 for to handle the 15th day Herba Eichhorniae blade with thalline, and 9,10 for to handle the 3rd day blade and contrast with toxin, and 11,12 for to handle the 3rd day plant and contrast with toxin.
Advantage of the present invention is: from natural environment, separate the Eichhornia crassipes chain lattice spore (Alternaria crassipes) that obtains, Be a new species of fungi of finding first and identifying, its mycelia segment and conidium inoculum are to the world's ten big malignant weed water Cucurbit (Eichhornia crassipes) has very strong pathogenic control action, and the toxin of its secretion also has this weeds The very strong toxicity effect that causes death. The microbial inoculum of germ and toxin all do not have harmful effect to other plant in important crops and the environment, Show the specialization removing activity of height, can be by the environment-friendly type fungoid of fermentation thalline or biomimetic synthesis technology production noresidue Herbicide has potential business development and using value, particularly has important in the biological control practice of water hyacinth Development prospect.
Embodiment
Below in conjunction with embodiment the present invention is further described
Embodiment 1:
Separation and purification of pathogenic bacteria and screening
(1) fungi separation and Culture potato sucrose nutrient agar (PSA), its prescription is the fresh potato 200g of peeling, each 20g of sucrose and agar adds tap water to 1000ml.It is well-done with potato to add water earlier, and double gauze filters the back and adds sucrose and agar, replenishes an amount of water to 1000ml, in the triangular flask of packing into after fully mixing evenly, puts into sterilizer sterilization back and places.Heating and melting before using is poured in sterilization culture dish or the test tube and is made flat board or slant medium, uses for fungi separation and Culture or bacterial classification preservation.
(2) separation and Culture and purifying: under natural condition, gather Herba Eichhorniae plant sample with typical disease symptom, the clean back of rinsing clip leaf spot lesion and healthy part are had a common boundary organizes little (2mm), put into 5% chlorine bleach liquor's surface sterilization and use the aqua sterilisa rinsing later on 3 times, place the PSA flat board, in 25 ℃ of incubators, cultivate and observation every day, treat to extract the mycelia tip respectively after different bacterium colonies grow in good time, transfer to and continue to cultivate into pure bacterium colony on the new PSA flat board, transfer to again on the PSA test tube slant substratum, and number and indicate bacterial strain, preserve standby.
(3) determine pathogenic bacteria: determine pathogenic bacteria according to the sick rule of Ke He Shi card step.Smear or the inoculation of acupuncture drop for healthy water cucurbit plant leaf with obtaining various fungies after the separation and purification of sick leaf texture, guarantee that lucifuge preserved moisture 24 hours,, observe incidence 28 ℃ of growths down.The fungi that causes the morbidity of inoculation blade, performance same symptoms can be defined as pathogenic bacteria.
(4) strong pathogenic bacterium screening: above-mentioned test is determined that the identical method of all pathogenic bacterias (smearing mist or acupuncture drop) is to the inoculation of Herba Eichhorniae plant, and cultivating the severity of observing disease on the inoculation plant under the identical suitable condition, relatively filter out the bacterial strain that causes that Herba Eichhorniae morbidity is serious, screening has obtained Herba Eichhorniae chain lattice spore thus.
Embodiment 2:
Cultivation of pathogenic bacteria and inoculation condition test
(2) humid test: cultivating on the PDA flat board of 5d with the punch tool cut-off directly is the mycelia piece of the AFT001 bacterial strain of 0.5cm, be inoculated on the PDA substratum, establish 5 ℃, 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃ and 40 ± 0.5 ℃ of 8 Temperature Treatment respectively, each is handled and repeats 3 times.Place 8 pre-set temperature required illumination boxs to cultivate, and measure the bacterium colony size day by day with vertical cross method, every 24h measures 1 time, continuously measured 5 times, record data.The potential of hydrogen of PDA substratum is about pH7.0.Determine the optimum growth temperature of alternaric bacteria thus.
(2) potential of hydrogen experiment: the PDA substratum is adopted in experiment, 11 potential of hydrogen is set altogether handles.With the NaOH of 1.0mol/L and HCl solution the pH value of substratum is adjusted to 4.0,5.0,6.0,6.5,7.0,7.5,8.0,9.0,10.0,11.0 and 12.0 (measuring with the electronics pH meter) respectively respectively, each is handled and repeats 5 times; Germ inoculation and growth measuring method are the same, connect the bacterium processing and are placed on 25 ℃ of cultivations down, and every 24h measures a colony diameter, continuously measured 6 times.Determine the optimal ph of chain lattice spore growth thus.
(3) screening of kinds of culture medium: the influence of 8 kinds of substratum to the AFT001 colony growth compared in experiment altogether, is respectively WA, CAA, PSA, PDA, the Herba Eichhorniae blade is fried in shallow oil juice substratum (WLA), medium oatmeal (OSA), WLA+PDA and WLA+PSA.Germ inoculation and growth measuring method are with aforementioned example.The potential of hydrogen of various substratum is about pH7.0, and culture temperature is 25 ℃.Determine the optimal medium that germ is cultivated thus.
(4) illumination is to the influence of sporulation quantity test: with the AFT001 inoculation that grows fine in PCA (potato 20g, Radix Dauci Sativae 20g, agar 20g) on the substratum, cultivate 20d under near ultraviolet lamp (NUV), fluorescent lamp (DL) and dark condition, each is handled and repeats 3 times.Collection spore counting, method is as follows: add the sterilized water of 1ml on substratum, gently scrape media surface with cover glass.Then spore suspension is filtered with 100 purpose mesh screens, get filter the spore liquid microscopy, count with blood counting chamber.Relatively the different light culture condition is to producing the influence of spore, define the light source that is beneficial to germ product spore.
(5) the germ inoculation condition is optimized: generally influence the shadow that plant pathogenic fungi infects and mainly contain temperature, moisture preserving time and illumination.Be positioned at 5 temperature between 20～30 ℃ according to optimal growth condition being provided with of aforementioned Herba Eichhorniae chain lattice spore among the present invention, preserve moisture (%) time 0,12,24,32 and 48h, illumination is set to 1200Lux, and 3000Lux is irradiation 0,6,12,18 and 24h down.Each Temperature Treatment 3 strain Herba Eichhorniae, every strain 5-6 blade.Analyze the suitable inoculation onset condition (temperature, moisture preserving time and illumination) of determining germ thus.
Embodiment 3:
The thick toxin of pathogenic bacteria separates
(1) preparation of germ culturing filtrate: pour 250ml potato sucrose (PS) nutrient solution in the 500ml triangular flask respectively into, the sterilization back is stand-by.Choose and cultivate the bacterium colony that 6～7d grows fine, get the bacterium cake of Φ 6mm size, be inoculated in the triangular flask that 250ml PS nutrient solution is housed, 8 of every bottle graft kinds, static cultivation 20 days under 12h illumination/sky condition in 25 ℃ of incubators with punch tool.On Bechtop, filter with qualitative filter paper (middling speed), can obtain culturing filtrate.Prepare the usefulness of enough germ culturing filtrates thus for the toxin separation and Extraction.
(2) extraction of toxin and activity test: select different organic solvent sherwood oil, chloroform, phenol and the ethyl acetate etc. of polarity respectively culturing filtrate to be extracted, method is: with the culturing filtrate of certain volume with isopyknic organic solvent extraction 3 times, merge organic phase then, use the Rotary Evaporators evaporation concentration, reclaim organic solvent.With the enriched material of every kind of organic solvent extraction with water dissolution after with doing that leaves of hyacinth sheet dip treating is measured its activity.The enriched product solution of result after with ethyl acetate extraction shows very strong toxicity to Herba Eichhorniae.
Embodiment 4:
Safety (specialization) the property mensuration of pathogenic bacteria and toxin thereof
(1) for the crop of testing: 20 kinds of important floods and droughts crops having selected 6 sections, comprise paddy rice gramineous, wheat, corn, the tomato of Solanaceae, capsicum and tobacco, cucumber cucurbitaceous and watermelon, the radish of Cruciferae, wild cabbage, rape and Chinese cabbage, the peanut of pulse family, pea, broad bean, soybean and mung bean, and the cotton of Malvaceae.
(2) seed germination test: the healthy seed of selecting aforementioned 20 kinds of crops, (the small-sized seed treatment time is short to use mycelia and spore suspension, the thick toxin soiutions of germ and clear water (contrast) to soak 12～36 hours respectively, the large seed treatment time is long), be placed on the wet filter paper, in 25 ℃ of growth casees, sprout back record seed germination rate.The result shows that the crop seed germination rate of being tested does not all have significant difference with contrast.
(3) crop plant growth test:, be placed on the wet filter paper vernalization in 25 ℃ of growth casees with warm water soaking 12～36h (deciding) according to seed size.Treat to be seeded in the potted plant alms bowl after plumule exposes.Add sandy soil in the alms bowl, add a certain amount of nutritive medium (adding amount of urea, ammonium sulfate and potassiumphosphate in the water) then through washing and hyperthermia drying.After planting grow under greenhouse experiment, use mycelia and spore suspension, the thick toxin soiutions of germ and clear water (contrast) spraying to handle when treating plant length to suitable size respectively, the while serves as the contrast of falling ill with treating water cucurbit plant.Handle the pathology situation of back each kind of plant of observed and recorded every day, results plant during by the 20th day, the increment of mensuration different treatment.The result shows, handle back Herba Eichhorniae plant with alternaric bacteria liquid and toxin solution and after processing, promptly showed tangible disease and toxin symptom respectively in the 7th day and the 2nd day, and all 20 kinds of crop plants after handling 20 days also without any pathology, do not have significant difference between the increment with growth increment after bacterium liquid and the toxin solution processing and clear water adjoining tree.This explanation germ and toxin thereof study thing without any detrimentally affect to supplying.
Claims (1)
1, a kind of water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata (Alternaria crassipes), it is characterized in that: bacterial strain is AFT001, the preserving number of this bacterial strain is CGMCC NO.2569.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200810237208XA CN101508959A (en) | 2008-12-23 | 2008-12-23 | Water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200810237208XA CN101508959A (en) | 2008-12-23 | 2008-12-23 | Water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101508959A true CN101508959A (en) | 2009-08-19 |
Family
ID=41001492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200810237208XA Pending CN101508959A (en) | 2008-12-23 | 2008-12-23 | Water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101508959A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061330A (en) * | 2010-11-30 | 2011-05-18 | 中国农业科学院油料作物研究所 | Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria |
| CN102648713A (en) * | 2011-02-23 | 2012-08-29 | 天津市植物保护研究所 | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein |
-
2008
- 2008-12-23 CN CNA200810237208XA patent/CN101508959A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061330A (en) * | 2010-11-30 | 2011-05-18 | 中国农业科学院油料作物研究所 | Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria |
| CN102061330B (en) * | 2010-11-30 | 2012-09-05 | 中国农业科学院油料作物研究所 | Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria |
| CN102648713A (en) * | 2011-02-23 | 2012-08-29 | 天津市植物保护研究所 | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101851597B (en) | Streptomyces griseoflavus for resisting alfalfa diseases and screening method thereof | |
| CN101822272A (en) | Streptomyces griseoflavus and application thereof in biological prevention and control of plant diseases | |
| CN105886405B (en) | Dendrobium candidum endogenetic fungus and its application | |
| CN104789509B (en) | Raw bacillus pumilus and its application in one plant of bark of eucommia | |
| CN106497852B (en) | Burkholderia strain ES-89 for preventing and treating plant fungal diseases as well as culture method and application thereof | |
| CN102532247B (en) | Nematicidal compound derived from trichoderma virens as well as preparation method and application thereof | |
| CN108795830A (en) | A kind of angstrom of lucky series bacillus（Paenibacillus elgii）SWL-W8 and its application | |
| CN104195064B (en) | The Oryza sativa L. endogeny rayungus of the one external efficient antagonism Pyricularia oryzae of strain | |
| CN102604857B (en) | Biological control pseudomonas monteilii strain against tobacco mosaic virus (TMV) | |
| CN113234642A (en) | Streptomyces thioluteus St-79 and application thereof | |
| CN115261233A (en) | Saffron stem rot biocontrol fungus and application thereof | |
| CN102876584A (en) | Xylaria strain and application thereof | |
| CN101555456A (en) | Fusarium philoceroides | |
| CN110408547B (en) | Trichoderma viride for preventing and treating phytophthora capsici, application and capsicum cultivation method | |
| CN108559718A (en) | The myxococcus stipitatus of one plant of predacious plant pathogenetic bacteria and its application in bacterial disease biological control | |
| CN102382776B (en) | Small spore phoma microspora for controlling conyza sumatrensis | |
| CN105028204B (en) | The screening technique of the anti-early blight of virus-free potato tube plant | |
| CN101508959A (en) | Water hyacinth fungal biocontrol agent-eichhornia crassipes alternaria alternata | |
| CN107164233A (en) | A kind of chaetomium globosum LJ S2L1 bacterial strains and its application | |
| CN105713841A (en) | Fusarium oxysporum and application of fusarium oxysporum in germination pretreatment of fagopyrum tataricum seeds | |
| CN115851553A (en) | Streptomyces virginiae capable of preventing and treating clubroot and application thereof | |
| Li et al. | A new species, Embellisia astragali sp. nov., causing standing milk-vetch disease in China | |
| CN102787085B (en) | Strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam and its application | |
| CN109077068A (en) | A kind of application of fungi rod method in prevention and control exotic invasive weed Yellow calla | |
| CN103399143B (en) | The method of Rapid identification anthracnose of tobacco resistance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090819 |