CN108559718A - The myxococcus stipitatus of one plant of predacious plant pathogenetic bacteria and its application in bacterial disease biological control - Google Patents
The myxococcus stipitatus of one plant of predacious plant pathogenetic bacteria and its application in bacterial disease biological control Download PDFInfo
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Abstract
The invention discloses the myxococcus stipitatus of one plant of predacious plant pathogenetic bacteria and its applications in bacterial disease biological control.The slime bacteria BS of one plant of antagonism plant pathogenetic bacteria, preserving number CCTCCNO.M2017496.Myxococcus stipitatus BS of the present invention inhibits the growth of the various plants pathogenetic bacterias such as Erwinia carotorora, common calla Bacteria erwinia, ralstonia solanacearum of tomato, blakleg of potato bacterium and erwinia amylovora by way of predation, shows the pathogenic bacteria ability of slime bacteria BS wide spectrums.Potted plant experiment shows that slime bacteria BS can effectively inhibit carrot soft rot Pectinatus carrot subspecies (Pectobacterium carotovorum subsp.carotovorum) to infect potato and common calla, reduce the incidence of soft rot, the biological control for the plant disease that can be used for caused by plant pathogenetic bacteria.
Description
Technical field
The invention belongs to applied microbiology field, be related to one plant of predacious plant pathogenetic bacteria myxococcus stipitatus and its
Application in bacterial disease biological control.
Background technology
Plant disease is one of the important restriction factor of crops good quality and high output, it is estimated that global staple crops are averaged
Disease loss accounts for about nearly the 14% of total output, and annual direct economic loss is up to hundreds billion of dollars.In order to cope with plant disease institute
Caused by endanger, chemical pesticide control and crop breeding have become important means at present, however ring caused by chemical pesticide
The limitation for the features such as border is polluted and breeding cycle is long so that environmentally friendly biological and ecological methods to prevent plant disease, pests, and erosion means become the focus of research.Microorganism gives birth to
Object Prevention Technique has the characteristics that efficient, pollution-free and soil definite value ability is strong, meets the requirement of agricultural sustainable development, by
Gradually become the important means of control of plant disease.
Bacterial soft rot can cause the generation of the plant soft rot diseases such as crop in cruciferae, tomato, potato, melon, such as
Soft rot of cabbage and common calla soft rot, the illness are by carrot soft rot Pectinatus carrot subspecies (Pectobacterium
Carotovorum subsp.carotovorum, abbreviation Pcc) cause.The disease early period arid, the later stage is rainy or pours water not
In the case of, easily causes plant root and rot, to bring larger economic loss to peasant.
Bacterial wilt generally occurs in various regions, especially occurs in south and rainy time more serious.Morbidity is tight
Caused when weight plant it is green it is withered die, cause the underproduction even to be had no harvest, such as bacterial wilt of tomato, when morbidity causes complete stool to be wilted.This illness
It is infected and is caused by bacterium Pseudomonas solanacearum Pseudomanas solanacearum (Smith).It can infect including tomato, eggplant
Various crop including son, capsicum, potato and ginger etc..
Fire blight of pear is the destructive disease on European pear tree, belongs to the important quarantine object of dangerous disease and China.
The host range of erwinia amylovora is wide, can endanger pears, apple, hawthorn, wooden ten days and Japanese plum etc. more than 40 and belong to more than 220 kinds of plant
Object.The disease is caused by Erwinia amylovora, becomes dark brown quickly after plant flowers, fruit and blade are infected by fiery vaccine
It is withered, like burning.
Chrysanthemum wilt Classification system is Erwinia chrysanthemi Dickeya chrysanthemi, and chrysanthemum erwinia is a kind of soft
Maize ear rot bacterium often endangers the pulp of plant, and such as stem tuber is transplanted, fleshy leaf.The germ can also colonize simultaneously in the xylem of plant
Systemic infection is formed, therefore and a kind of vascular bundle causes wilting germ, special mode of infection to cause currently used chemical drug
Method can not effectively prevent it.
Bleeding canker (Dickeya fangzhongdai) also belongs to bacterial disease, has concealment, burst and ruins
The characteristics of going out property, appearance symptom can not judge whether there is the disease before occurring, symptom causes irreparable damage Yi Dan occurred.
Less serious case's branch is withered, trunk generates scab, and entire plant is dead when serious.It is had resulted in the fruit tree kinds plant such as pear tree at present tight
The economic loss of weight.In addition the balck shank caused by Dickeya solani is also a kind of more serious bacterial disease, disease
Bacterium can be in the soil plant residue on or the sick potato of storage period on it is overwintering.Blakleg of potato is common are, wild cabbage is black
Shin disease, fish pelargonium balck shank etc..
The planting type of traditional pest control method such as crop rotation can effectively reduce occurring degree, but cannot be fundamentally
It solves disease and also brings huge pollution and destruction to environment, while passing through life although chemical pesticide can remove disease at all
The enrichment of object chain can also threaten to health.Therefore, by seeking novel diseases prevention, means of curing the disease, reach effective control
Pathogenetic bacteria disease processed, it has also become project in the urgent need to address in production practices.Efficient, safety, ring based on biological control
The advantages that guarantor and people the demand to grain and salad vegetable, take biological control method to have become coke of people's attention
Point problem.
Microorganism biocontrol microorganisms are mainly that the Trichoderma harzianum of Mycophyta, pythium oligandrum, viscous broom be mould and Paecilomyces varioti etc. at present, bacterium
Bacillus and pseudomonad of class etc. additionally include the low virulent strain system etc. of some actinomyces and virus.Slime bacteria
(myxobacteria) it is a kind of prokaryotes with complicated many cells behavior and form, there is complicated many cells behavior
The generation ability of feature and abundant production secondary metabolite.Slime bacteria has as novel biocontrol bacterial strain to be better than having reported life
Fungi-proofing feature, including good microorganism prey ability, soil colonization ability, abundant novel cometabolism production can be generated
Object can generate the strong gloeospore of resistance and participation soil organic matter metabolism etc., these features make slime bacteria as life
It is fungi-proofing that there is good advantage.It is more for the research of slime bacteria auxology feature and secondary metabolite at present, and slime bacteria
Research and application in terms of the biological control of phytopathogen is less, wherein the mainly micro- life in Guangdong Province as patent protection
It is prepared by object apllied Myxococcus sp.e-3-1, Polyangium sp.8#-3 and Cystobacter sp.XJ9-1
Application (201611095485.2) etc. in the drug of predation and inhibition plant pathogenetic bacteria.Therefore, slime bacteria is in prevention plant
There is larger application space in terms of pathogenetic bacteria disease.However there are larger differences for the antibacterial effect between different genera bacterial strain
It is different, as bacillus shows that different antibacterial characteristics, related strain have also applied for patent protection in terms of anti-phytopathogen
(201380042989.6；201410164779.0 and 201510666311.6 etc.).Therefore the patent based on existing biocontrol microorganisms is protected
Present situation is protected, especially slime bacteria is as a kind of Biocontrol microorganism with application potential, different characteristics and with good biological and ecological methods to prevent plant disease, pests, and erosion energy
The screening and patent protection of the novel slime bacteria of power have great importance for the biological control of plant disease.
Invention content
The present invention provides one plant of slime bacteria Myxococcus stipitatus BS answering in preventing plant pathogenetic bacteria
With.
The slime bacteria BS of one plant of antagonism plant pathogenetic bacteria, Classification And Nomenclature are myxococcus stipitatus (Myxococcus
Stipitatus), it is preserved in China typical culture collection center, preservation address is Wuhan, China, Wuhan University, preservation date
For September in 2017 12 days, preserving number CCTCCNO：M2017496.
Culture, bacterial strain bacterium solution, strain fermentation culture solution or the fermentation culture of slime bacteria bacterial strain BS of the present invention
Filtered fluid.
Slime bacteria BS of the present invention is the bacteria agent and bio-feritlizer of active constituent.
Applications of the slime bacteria BS of the present invention in preventing plant pathogenetic bacteria, wherein the pathogenetic bacteria includes
Carrot soft rot Pectinatus carrot subspecies Pectobacterium carolovora subsp.Carolovora, it is green withered false single
Born of the same parents bacterium Pseudomanas solanacearum (Smith), Erwinia chrysanthemi Dickeya chrysanthemi, bleeding canker
Bacterium Dickeya fangzhongdai, black shank bacterium Dickeya solani or erwinia amylovora Erwinia amylovora
(Ea)。
Culture, bacterial strain bacterium solution, strain fermentation culture solution or the fermentation culture of slime bacteria bacterial strain BS of the present invention
Application of the filtered fluid in preventing plant pathogenetic bacteria, wherein the pathogenetic bacteria include carrot soft rot Pectinatus recklessly
Radish subspecies Pectobacterium carolovora subsp.Carolovora, Pseudomonas solanacearum Pseudomanas
Solanacearum (Smith), Erwinia chrysanthemi Dickeya chrysanthemi, bleeding canker bacterium Dickeya
Fangzhongdai, black shank bacterium Dickeya solani or erwinia amylovora Erwinia amylovora.
The application preferably cultivates the slime bacteria BS, obtains strain culture, bacterial strain bacterium solution, bacterial strain
The filtered fluid of fermentation culture or fermentation culture；With obtained strain culture, bacterial strain bacterium solution, strain fermentation culture solution or
The filtered fluid of fermentation culture is applied to the biological control of the plant pathogenetic bacteria.
In the present invention slime bacteria Myxococcus stipitatus BS in potted plant experiment to carrot soft rot Pectinatus
Caused potato and common calla soft rot all shows good biocontrol effect.
Advantageous effect
The present invention successfully screens one plant of slime bacteria bacterial strain using rabbit excrement fruiting-body inducement method from the soil sample acquired
BS, is identified as myxococcus stipitatus (Myxococcus stipitatus), which can be with various plants disease in growth course
Indigenous bacteria is food, and cracking pathogen provides nutriment for own growth breeding.The slime bacteria is to various plants pathogenetic bacteria
Good prey ability shows its resistance of wide spectrum to pathogenetic bacteria.Based on predation experiment and potato and common calla potted plant experiment,
It is huge to show that slime bacteria Myxococcus stipitatus BS of the present invention have in terms of plant pathogenetic bacteria prevention
Application potential.
Description of the drawings
Fig. 1 bacterial strains purify flow chart
The characteristic picture of Fig. 2 bacterial strains
A figures are the fructification structure chart that bacterial strain BS is formed；B figures are bacterial strain BS colonial morphology figures；C figures are bacterial strain BS thalline shapes
State figure (100 ×)；D figures are thalline transmission electron microscope picture.
Fig. 3 Myxococcus stipitatus BS have good prey ability to a variety of pathogenetic bacterias
A slime bacterias BS preys on experiment to the tablet of various plants pathogenetic bacteria；After b BS predation various plants pathogenetic bacterias
Pathogenetic bacteria viable bacteria data statistics.Plant pathogenetic bacteria is erwinia amylovora；Pear tree bleeding canker bacterium；Chrysanthemum wilt；
Solanaceae black shank bacterium；Common calla Bacteria erwinia and ralstonia solanacearum of tomato.
The influence that Fig. 4 bacterial strain BS cultivation and fermentations liquid grows Pcc.
CK is the biomass for originating Pcc；BS supernatants are bacterial strain BS supernatants；Heat inactivation is to be carried out to bacterial strain BS supernatants
Supernatant after 100 DEG C of heat treatments.
Control effects of the bacterial strain BS to common calla soft rot in Fig. 5 potted plant experiments.
The growing state of common calla in a potted plant experiments；The Decay analysis of common calla rhizome ball in b potted plant experiments.
Biomaterial preservation information
Myxococcus stipitatus BS (Myxococcus stipitatus BS), is preserved in China typical culture collection center,
Preservation address is Wuhan, China, Wuhan University, and the deposit date is Septembers in 2017 12 days, and preserving number is CCTCC NO：
M2017496。
Specific implementation mode
1. bacterial strain of embodiment isolates and purifies
Natural air drying (pollution for reducing mould and miscellaneous bacteria) at room temperature after 1.1 sample collections.
Soil sample sample natural air drying at ambient temperature is acquired from Anhui and other places.
The induction of 1.2 slime bacteria fructifications
1.2.1 faecal pellet induces flat band method：By about 20 grams air-dried of soil sample magnificence in culture dish, it is embedded on soil sample surface half
3-4 sterilized rabbit excrement are added the cycloheximide (25mg/ml) or carbendazim (30mg/l) of the sterilizing of 25 μ g/ml ether, impregnate
After 6h, raffinate is removed, 30 DEG C of heat insulating cultures start to observe the formation of fructification on excrement ball after 48h.
1.2.2 yeast induces flat band method：Using WCX (cycloheximide 25mg/ml) culture medium as basic culture medium, nothing is poured into
Bacterium tablet puts a small amount of soil sample in media surface yeast mark signature line at cross hairs center, 30 DEG C of constant temperature incubations, is opened after 72h
The formation for the observation fructification that begins.
1.3 rabbit excrement induce the formation of fructification
1.3.1 slime bacteria isolates and purifies
1. the fructification induced with capillary tip picking 1.2.1 is inoculated in YWCX (WCX culture mediums：0.1%
CaCl2·2H2O；1.5%Agar；25 μ g/ml cycloheximide solution；0.1% crystal violet；PH value 7.2) on culture medium (with going out
Bacterium yeast intersects scribing line), 30 DEG C of cultures.
2. as found worm, by the colony inoculation of initial gross separation purifying in VY/4 (Angel Yeast 0.5%, CaCl2·2H2O
0.1%, vitamin B120.5 μ g/ml, agar powder 1.5%, 0.1% crystal violet；pH 7.2；) on slant medium, set -80
DEG C refrigerator freezing 48h is transferred method after taking-up using YWCX culture mediums, repeatedly again immediately until obtaining pure bacterial strain.
3. with the bacterium of the doubtful colony edge of the timely picking of capillary tip, transferred repeatedly in the flat of the culture medium containing VY/4
Plate, 30 DEG C of constant-temperature moisture-keeping cultures.Such as find worm, by initial gross separation purifying colony inoculation in set on VY/4 slant mediums-
80 DEG C of refrigerator freezing 48h.
1.4 LB culture mediums verify purity
Slime bacteria BS after purification is inoculated in LB culture mediums (tryptone 1%, NaCl 1%, yeast extract
0.5%) in, 30 DEG C of shaken cultivations are incubated overnight liquid clarification and show that the slime bacteria is pure bacterial strain.
The functional verification and culture presevation of 1.5 species strains
1.5.1 the bacterial strain BS of purifying is inoculated on VY/4 solid plate culture mediums, checks the formational situation of transparent circle.
1.5.2 rabbit excrement is dried in vacuo preserving process.The strain of purifying is inoculated on the food lepus europaeus excrement sterilized through high-temperature sterilization,
Rabbit excrement with fructification is put into vacuum desiccator and is preserved when fructification occurs by 30 DEG C of 5-8d of culture.
The identification of 2. bacterial strain of embodiment
2.1 morphological feature
The slime bacteria BS that separation screening obtains shows feminine gender through Gram's staining, by violet staining in oily microscopic observation
It was found that the form of single bacterium is in rod-shaped, atrichia, both ends are transparent, and being observed under transmission electron microscope around thalline has one layer of rete malpighii；
Find that the bacterial strain in the process of growth there is population effect, Later growth bacterium colony to flock together to be formed by light microscopic 10 × observation
Fructification (Fig. 2).
2.2 identification
By the PCR amplification to bacterial strain 16S rDNA, the 16S rDNA gene orders of the bacterium are obtained, log in NCBI
(www.ncbi.nlm.nih.govPblastP), the 16S rDNA sequences of typical strain similar with experimental strain are obtained.By institute
Sequencing result and known array in database are compared using BLAST softwares in GenBank databases, pass through sequence ratio
To the physiological and biochemical analysis of analysis and routine, which is initially identified as Myxococcus stipitatus, is named as BS.
Slime bacteria Myxococcus sp.e-3-1 described in patent of invention (201611095485.2) are isolated from Aksu of Xinjiang salt-soda soil
Soil, this area's Soil salinity is 233.8g/kg (Zhang Xianjiao etc., 2012) according to the literature, illustrates that the bacterium can be higher
Variation of Salinity Condition under grow.Slime bacteria Myxococcus stipitatus BS of the present invention in 233.8g/kg and
It is not grown under the Variation of Salinity Condition of 200g/kg, shows Myxococcus stipitatus BS of the present invention in growth characteristics
There are significant differences with the slime bacteria Myxococcus sp.e-3-1 described in patent of invention (201611095485.2) for aspect, and
Non- same bacterium.
Research of 3. slime bacteria of embodiment (Myxococcus stipitatus BS) to plant pathogenetic bacteria predation
3.1 bacterial strain BS study various plants pathogenetic bacteria predation.
Ea, Dickeya fangzhongdai, Dickeya chrysanthemi, Dickeya solani, Pcc, RS connect
In LB liquid medium (tryptone 1%, NaCl 1%, yeast extract 0.5%), 30 DEG C are incubated overnight kind,
8000rpm centrifuges 3min, collects thalline, makes its OD with sterile water resuspension600It is 10.By its hanging drop to TPM (10mM Tris-HCl
[pH7.6],1mM KH2PO4[pH 7.6],8mM MgSO4)) on solid plate drying it is for use.By bacterial strain BS (CCTCC
M2017496 CYE fluid nutrient mediums (Casitione 1%, Yaest extract 0.5%MgSO) are inoculated into4·7H2O
0.1%pH 7.2-7.6) in, 30 DEG C are cultivated 2 days, and 8000rpm centrifuges 3min, collect thalline, and thalline, thalline is resuspended with sterile water
A concentration of 108Cells/l, for hanging drop on the pathogenetic bacteria lawn of drying, 30 DEG C of cultures observe slime bacteria spread scenarios in 3-5 days,
It is cloudy compare not connect slime bacteria.After culture 1 day, slime bacteria, which can be observed, to be spread by nutriment of plant pathogenetic bacteria
Property growth.After culture 3 days, slime bacteria can diffuse to pathogenetic bacteria colony edge completely, and across region form son
Entity illustrates that slime bacteria has good predation characteristic.Entire bacterium colony is scraped at this time, it is flat in LB solids by method of dilution butteron on plate
The residual quantity of plant pathogenetic bacteria is counted in plate.The residual quantity of various pathogenetic bacterias is from the order of magnitude 10 before8Drop to 106.As a result
Show that slime bacteria BS all shows good prey ability to various plants pathogenetic bacteria, shows that its good broad-spectrum disease resistance is former thin
Bacterium ability (Fig. 3).
The influence that 3.2 bacterial strain BS fermentation cultures grow carrot soft rot Pectinatus carrot subspecies Pcc
Bacterial strain BS (CCTCC M2017496) is inoculated into CYE fluid nutrient mediums (Casitione 1%, Yaest
Extract 0.5%MgSO4·7H2O 0.1%pH 7.2-7.6) in, 30 DEG C are cultivated 2 days, 8000rpm centrifugation 5min, in collection
Clear liquid and remaining microorganism is removed with bacterial filter, is mixed with supernatant zymotic fluid and Pcc after standing 30 DEG C of culture 16h after bacterium,
Dilution spread counts Pcc residual quantities, boils processing and saline control with heat.Statistics indicate that life of the zymotic fluid of BS to Pcc
It is long that also there is partial inhibition (Fig. 4).
The influence that the secondary metabolite of 3.3 bacterial strain BS grows Pcc
With reference to the preparation method in patent of invention (201611095485.2), VY/2 culture mediums (5g Angel active dry yeasrs,
Add boiling to boil 10min, 1g MgSO are added after cooling4,1g CaCl2, pH to 7.4 is adjusted, 1L, high-temperature heat sterilization are settled to) in
It is inoculated with slime bacteria BS, 30 DEG C, is cultivated 5 days in 180rpm, thalline and fermented liquid supernatant are collected in 8000rpm centrifugations.Zymotic fluid use etc.
The ethyl acetate of volume extracts 12h, after extract liquor rotary evaporation, with methanol dissolution extraction object, obtains zymotic fluid extract.Thalline
With ultrasonic disruption after acetone soak, 12h is then extracted with ethyl acetate and, with methanol dissolution extraction object, is obtained after extract liquor rotates
To bacterial cell disruption liquid extract.Zymotic fluid extract and bacterial cell disruption liquid extract be dissolved into respectively with methanol 50mg/mL and
100mg/mL concentration.Pathogen is inoculated with to LB liquid medium 180rpm, logarithmic phase is arrived in 30 DEG C of cultures.By the phytopathy of logarithmic phase
Opportunistic pathogen (Pcc) solution by volume 1：100 ratios and 50 DEG C or so of 3mL LB semi-solid agars culture mediums (0.75%agar)
Mixing, bed board after shaking.The zymotic fluid extract that 5uL various concentrations have been added dropwise or the extraction of bacterial cell disruption liquid are adhered on tablet
The 6mm diameter filter papers of object solution.Antibacterial circle diameter is measured with after 37 DEG C of culture 24-36h.As a result, it has been found that zymotic fluid extract exists
50mg/ml concentration is 5.5 ± 0.3mm to the inhibition zone of Pcc, and bacterial cell disruption liquid extract is in 50mg/ml concentration to the antibacterial of Pcc
Circle is 6.5 ± 0.5mm.The Myxococcus that the antibacterial property compares involved in patent of invention (201611095485.2)
There are significant differences by sp.e-3-1, i.e., secondary metabolites have good bacteriostasis in Myxococcus sp.e-3-1, and
What the antibacterial effect for the Myxococcus stipitatus BS that this patent is related to mainly was realized by the predation of slime bacteria.
The application assessment of 4. slime bacteria of embodiment (Myxococcus stipitatus BS) anti-plant pathogenetic bacteria
In order to study Biocontrol Effects of the slime bacteria BS according to the present invention in actual application to plant pathogenetic bacteria,
The present invention chooses carrot soft rot Pectinatus carrot subspecies (Pcc) and is used as research material, with potato block experiment and common calla basin
Plant biological and ecological methods to prevent plant disease, pests, and erosion abilities of the experimental verification slime bacteria BS under soil environment.
4.1 soft rot infect potato block experiment
The thalline that 3.1 collect is resuspended with sterile water to a concentration of 3 × 108It is inoculated in the soil of infection soft rot Pcc,
Pass through potato tubers infection pathogen degree (tissue rots, and calculates rotting rate) assessment Myxococcus stipitatus BS
The effect of prevention, using do not meet BS and Chemical treatment as reference.After being placed at room temperature for the 5th day, Pcc group rotting rates reach
45.05%, and slime bacteria Myxococcus stipitatus BS group rotting rates is added to be 0, and after the 10th, the other corruption of Pcc groups
Rotten rate reaches 96.71%, almost rots, and the incidence of slime bacteria processing group is in 18.39% (table 1).Illustrate in soil
Under environmental condition, slime bacteria BS can be good at inhibiting infecting for pathogen Pcc, reduce the incidence of soft rot.
Control effects of the 1 bacterial strain BS of table to potato tubers soft rot
| Processing | Initial incidence (%) | 5th day incidence (%) | Tenth day incidence (%) |
| Water | 0±0 | 2.15±0.029a | 27.51±0.113b |
| Pcc | 0±0 | 45.05±0.104b | 96.71±0.156a |
| Pcc+Agricultural-streptomycin | 0±0 | 14.98±0.065a | 88.69±0.088a |
| Agricultural-streptomycin | 0±0 | 0±0a | 13.59±0.04b |
| BS | 0±0 | 0.93±0.012a | 14.78±0.152b |
| Pcc+BS | 0±0 | 0±0a | 18.39±0.183b |
4.2 common calla potted plant experiments
By bacterial strain BS shake flask fermentations 3 days, it is inoculated in the root of the calla lily infected by soft rot, one group is soaked
Another group is not added with water while irrigating BS bacteria suspensions, compares as clear water.Incidence, later stage statistics are observed in growing process
Common calla stem tuber rots situation.Statistics indicate that plus BS bacteria suspensions processing group, plant growing way is fine, does not fall ill, and control group send out
Sick serious, plant part is dead.Later stage collects common calla stem tuber, statistics block stem Decay.The result shows that two control groups
The equal ghost of stem tuber is rotted.And the stem tuber of BS bacteria suspension processing groups is substantially intact, and partial block stem length goes out new ball (Fig. 5).Illustrate
In soil environment, slime bacteria BS can inhibit Pcc to infect common calla root, reduce the incidence of soft rot, even if in height
Under the environmental condition of humidity, good biological control efficiency is still shown, show the good Biocontrol Potentials of bacterial strain BS.
Claims (6)
1. the slime bacteria BS of one plant of antagonism plant pathogenetic bacteria, Classification And Nomenclature is myxococcus stipitatus (Myxococcus
Stipitatus), it is preserved in China typical culture collection center, preservation address is Wuhan, China, Wuhan University, preservation date
For September in 2017 12 days, microbial preservation number was CCTCCNO.M2017496.
2. culture, bacterial strain bacterium solution, strain fermentation culture solution or the fermentation culture of slime bacteria bacterial strain BS described in claim 1
Filtered fluid.
3. slime bacteria BS described in claim 1 is the bacteria agent and bio-feritlizer of active constituent.
4. applications of the slime bacteria BS described in claim 1 in preventing plant pathogenetic bacteria, wherein the pathogenetic bacteria packet
Carrot soft rot Pectinatus carrot subspecies Pectobacterium carotovorum subsp.carotovorum are included, it is green withered
Pseudomonad Pseudomanas solanacearum (Smith), Erwinia chrysanthemi Dickeya chrysanthemi, bleeding are burst
Ulcer germ Dickeya fangzhongdai, black shank bacterium Dickeya solani or erwinia amylovora Erwinia
amylovora。
5. culture, bacterial strain bacterium solution, strain fermentation culture solution or the fermentation culture of the slime bacteria bacterial strain BS described in claim 2
Application of the filtered fluid in preventing plant pathogenetic bacteria, wherein the pathogenetic bacteria include carrot soft rot Pectinatus recklessly
Radish subspecies Pectobacterium carotovorum subsp.carotovorum, Pseudomonas solanacearum Pseudomanas
Solanacearum (Smith), Erwinia chrysanthemi Dickeya chrysanthemi, bleeding canker bacterium Dickeya
Fangzhongdai, black shank bacterium Dickeya solani or erwinia amylovora Erwinia amylovora.
6. application according to claim 5, characterized in that including slime bacteria BS described in claim 1 is cultivated,
Obtain strain culture, bacterial strain bacterium solution, strain fermentation culture solution or fermentation culture filtered fluid；With obtained strain culturing
Object, bacterial strain bacterium solution, strain fermentation culture solution or fermentation culture filtered fluid be applied to the biology of the plant pathogenetic bacteria
Prevention.
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| CN109182213A (en) * | 2018-10-16 | 2019-01-11 | 云南省烟草公司普洱市公司 | A kind of tobacco-growing soil Sphingol single-cell X1-8 and its application in tobacco black shank prevention and treatment |
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| CN113528395A (en) * | 2021-08-10 | 2021-10-22 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Myxococcus xanthus preying on tomato ralstonia solanacearum and application of myxococcus xanthus in biological prevention and control of tomato bacterial wilt |
| CN113528395B (en) * | 2021-08-10 | 2023-05-12 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Myxococcus xanthus prey on tomato bacterial wilt and application thereof in biological prevention and control of tomato bacterial wilt |
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