CN101440356B - Bacillus licheniformis 202 and use thereof - Google Patents
Bacillus licheniformis 202 and use thereof Download PDFInfo
- Publication number
- CN101440356B CN101440356B CN2008101830752A CN200810183075A CN101440356B CN 101440356 B CN101440356 B CN 101440356B CN 2008101830752 A CN2008101830752 A CN 2008101830752A CN 200810183075 A CN200810183075 A CN 200810183075A CN 101440356 B CN101440356 B CN 101440356B
- Authority
- CN
- China
- Prior art keywords
- bacillus licheniformis
- pathogens
- banana
- application
- wilt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses Bacillus licheniformis 202, which has been preserved in China Center for Type Culture Collection, Wuhan University, Luojiashan Street, Wuchang District, Wuhan City, Hubei on July 2, 2007, wherein the preserving number is CCTCC NO: M207093. The invention also discloses a preparation method and application thereof. A Bacillus licheniformis 202 strain is obtained by screeningthrough plate confronting and pot experiment, can effectively inhibit the growth of banana vascular wilt pathogens, has a wide antimicrobial spectrum, has antagonistic action on a plurality of pathogenic fungi such as cotton wilt pathogens, cotton verticillium wilt pathogens, watermelon wilt pathogens, tomato wilt pathogens, curvularia leaf spot pathogens or banana anthracnose pathogens, and has good environmental safety and good development and application prospect.
Description
Technical field
The present invention relates to the plant protection field, relate in particular to a kind of interior biocontrol strain of giving birth to, relate in particular to a kind of bacillus licheniformis 202 and application of high-efficiency prevention and control banana blight.
Background technology
Banana blight is to infect a kind of destructive disease that causes by Fusarium oxysporum Cuba specialized form (Fusarium oxysporum f.sp.cubense), is the crushing soil-borne disease of banana production.Pathogenic bacteria is invaded from injured rhizome, spreads to false stem and leaf portion by vascular bundle, stops up xylem vessel, until the plant withered death.Banana blight bacteria is a soil inhabitant, and its chlamydospore can survive in soil 8～10 years.Banana blight all has distribution in a plurality of counties and cities in Delta of the Pearl River banana producing region, and the trend that develops rapidly is arranged in recent years.The sick garden of this disease diseased plant rate generally is about 10%～40%, and serious reaches more than 90%, so that Jiao Yuan lies waste, and is very harmful.
At present the control of banana blight still there are not desirable medicament and disease-resistant bacterial classification, some cultivation management measures can only be played partial control action kou, seek a kind of economy, the safe and effective method of preventing and treating is the task of top priority, in recent years, the research of beneficial rhizosphere bacterium provides new way for the biological control of banana blight, many beneficial rhizosphere bacteriums also can produce system's resistance by inducing plant, make plant obtain the broad-spectrum disease resistance ability.The protective effect safety that beneficial rhizosphere bacteria-induction plant produces and effectively, in biocontrol of plant disease, have important use and be worth.
Separation screening obtains biocontrol bacteria bacterial strain that banana blight bacteria is had antagonistic action in the banana plant, and is significant to the control of banana blight, but correlation technique is still very weak even blank at present.
Summary of the invention
The objective of the invention is blank at prior art, a kind of bacillus licheniformis 202 is provided, be a kind of in the banana plant separation screening obtain biocontrol bacteria bacterial strain that banana blight bacteria is had antagonistic action, lower concentration can effectively suppress the growth of banana blight bacteria down, and is significant to the control of banana blight.
Another object of the present invention has provided the preparation method of the bacillus licheniformis 202 of described high-efficiency prevention and control banana blight.
The bacillus licheniformis 202 that a further object of the invention has provided described high-efficiency prevention and control banana blight is in the multiple application that prevents aspect the banana disease disaster.
Technical scheme of the present invention provides a kind of bacillus licheniformis 202 of high-efficiency prevention and control banana blight, described bacillus licheniformis 202, Bacillus licheniformis 202, in on July 2nd, 2007 in the typical culture collection center preservation of Wuhan, Hubei loujia hill belongs Wuhan University China, preserving number is CCTCC NO:M207093.
The bacillus licheniformis 202 of described high-efficiency prevention and control banana blight obtains through flat board face-off screening.
The present invention also provides the application of the bacillus licheniformis 202 of described high-efficiency prevention and control banana blight, and the first is to the dull and stereotyped antagonistic action of No. 4 microspecies of banana blight bacteria.
It two is the application that prevent banana plant primary roots and brownization of vascular bundle or rotten aspect.
It three is antagonistic actions to the curved spore leaf spot fungi of tomato wilt bacterium, corn, glue spore anthrax bacteria, cotton-wilt fusarium, verticillium dahliae, fusarium graminearum or withered germ of water-melon.
The invention has the beneficial effects as follows:
(1) bacillus licheniformis 202 is an endophyte of plant, strong to the banana blight bacteria restraining effect, multiple pathogenic fungi had antagonistic action, has the important effect that antagonistic bacterium is played in biocontrol of plant disease, for example kind and One's name is legion are in the plant rhizosphere root, rhizosphere exists in a large number; The mode of action to pathogenic bacteria is varied; Advantages such as reproduction speed is fast, the present invention can effectively suppress the growth of banana blight bacteria by experimental results demonstrate the bacillus licheniformis 202 bacterial strain, antimicrobial spectrum is wide, and multiple pathogenic fungies such as cotton-wilt fusarium, verticillium dahliae, withered germ of water-melon, tomato wilt bacterium, curved spore leaf spot fungi, Glorosprium musarum Cookeet Mass are all had antagonistic action; Environmental safety is good, has the excellent development application prospect, and is especially significant to the control of banana blight.
(2) simple, the preservation easily of strain culturing condition of the present invention is easy to suitability for industrialized production, has the excellent development application prospect.
Description of drawings
Fig. 1 bacillus licheniformis 202 is to the restraining effect of FOC4 mycelia
Banana seedlings vascular bundle symptom after Fig. 2 inoculates 30 days
Fig. 3 bacillus licheniformis 202 is to the restraining effect of tomato wilt bacterium
Fig. 4 bacillus licheniformis 202 is to the restraining effect of glue born of the same parents anthrax-bacilus
Fig. 5 bacillus licheniformis 202 is to the restraining effect of Glorosprium musarum Cookeet Mass
Fig. 6 bacillus licheniformis 202 is to the restraining effect of cotton wilt
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment and accompanying drawing.
Described bacillus licheniformis 202, Bacillus licheniformis 202, on July 2nd, 2007 in the typical culture collection center preservation of Wuhan, Hubei loujia hill belongs Wuhan University China, preserving number is CCTCC NO:M207093.
Embodiment 1 bacillus licheniformis 202 is to the dull and stereotyped antagonistic action experiment of No. 4 microspecies FOC4 of banana blight bacteria
Inserting diameter in the dull and stereotyped central authorities of potato sucrose substratum PDA is the FOC4 mycelia piece of 5mm, on the triangle of equidistant FOC4 mycelia piece, put into the sterilization filter paper that diameter is 5mm subsequently, insert bacillus licheniformis 202 nutrient solution 10 μ L on the every filter paper, flat board with earth-free clothing sporeformer 202 nutrient solutions is contrast, cultivated 5～7 days in 25～28 ℃ of incubators, measure antibacterial circle diameter, drawings attached 1 visible bacillus licheniformis 202 is strong to the banana blight bacteria restraining effect.
Embodiment 2 bacillus licheniformis 202s prevent banana plant primary roots and brownization of vascular bundle or the experiment of rotting, greenhouse pot culture preventive effect.
Select the Brazilian banana seedling about plant height 15cm, carry out 3 kinds of different treatment: the contrast of (1) clear water healthy tree: directly transplant after the banana seedlings root is immersed in the sterilized water and in nutrition pot, grow; (2) the banana seedlings root is immersed in the bacillus licheniformis 202 nutrient solution behind the 30min, transplants to nutrition pot, again the FOC4 spore suspension of 20mL is irritated root and insert in the nutrition pot soil; (3) morbidity contrast: the banana seedlings root is irritated the spore suspension of root inoculation 20mLFOC4, does not inoculate the bacillus licheniformis 202 nutrient solution.More than handle every treatment group 20 strains.26 ℃ of cultivations afterwards are respectively at inoculation back 10d, 20d, 30d investigation sickness rate and disease index, three repetitions.Compared with the control, bacillus licheniformis 202 can reduce the occurrence degree of banana blight effectively, and effect is the most obvious behind the inoculation 30d, root and brownization of vascular bundle, the degree of rotting obviously alleviate, see accompanying drawing 2, wherein A: clear water inoculation contrast, no brown stain, no putrefactive phenomenon; D, E: inoculate bacillus licheniformis 202 inoculation liquid and FOC4 spore suspension simultaneously, slight brown stain, nothing are rotted; F, G, H, I: only inoculate the FOC4 spore suspension, serious brown stain, rotten.
Embodiment 3 bacillus licheniformis 202s are to the antagonistic action of multiple pathogenic bacteria
Adopt dull and stereotyped face-off method, the test bacillus licheniformis 202 is to the tomato wilt bacterium, corn is bent the spore leaf spot fungi, glue spore anthrax bacteria, cotton-wilt fusarium, verticillium dahliae, fusarium graminearum, the antagonistic effect of 7 kinds of pathogenic bacterias such as withered germ of water-melon, inserting diameter in the dull and stereotyped central authorities of PDA is the target mycelia piece of 5mm, on the triangle of equidistant FOC4 mycelia piece, put into the sterilization filter paper that diameter is 5mm subsequently, insert bacillus licheniformis 202 nutrient solution 10 μ L on the every filter paper, flat board with earth-free clothing sporeformer 202 nutrient solutions is contrast, cultivate 5～7d in 28 ℃ of incubators, measure antibacterial circle diameter.The bacillus licheniformis 202 antimicrobial spectrum is wider, and multiple pathogenic fungies such as cotton-wilt fusarium are had antagonistic action, sees Table 1 and accompanying drawing 3.From accompanying drawing 3, can see the restraining effect of bacillus licheniformis 202 to the tomato wilt bacterium; Can be observed the restraining effect of bacillus licheniformis 202 in the accompanying drawing 4 to glue born of the same parents anthrax-bacilus; Can be observed the restraining effect of bacillus licheniformis 202 in the accompanying drawing 5 to Glorosprium musarum Cookeet Mass; Can be observed the restraining effect of bacillus licheniformis 202 in the accompanying drawing 6 to cotton wilt.
Table 1: bacillus licheniformis 202 is to the restraining effect of multiple pathogenic bacteria
-: the unrestraint effect+: antibacterial circle diameter 1～4mm
++: antibacterial circle diameter 4～8mm +++: antibacterial circle diameter〉8mm
Claims (4)
1. bacillus licheniformis 202, on July 2nd, 2007 in the typical culture collection center preservation of Wuhan, Hubei loujia hill belongs Wuhan University China, preserving number is CCTCC NO:M207093.
2. the application of the described bacillus licheniformis 202 of claim 1 is characterized in that the dull and stereotyped antagonistic action to No. 4 microspecies of banana blight bacteria.
3. the application of the described bacillus licheniformis 202 of claim 1 is characterized in that preventing the application of banana plant primary roots and brownization of vascular bundle or rotten aspect.
4. the application of the described bacillus licheniformis 202 of claim 1 is characterized in that the antagonistic action to tomato wilt bacterium, the curved spore leaf spot fungi of corn, glue spore anthrax bacteria, verticillium dahliae, fusarium graminearum or withered germ of water-melon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101830752A CN101440356B (en) | 2007-12-04 | 2008-12-02 | Bacillus licheniformis 202 and use thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710032050.8 | 2007-12-04 | ||
| CNA2007100320508A CN101186886A (en) | 2007-12-04 | 2007-12-04 | Bacillus licheniformis 202 for highly effective preventing and curing banana wilt and application thereof |
| CN2008101830752A CN101440356B (en) | 2007-12-04 | 2008-12-02 | Bacillus licheniformis 202 and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101440356A CN101440356A (en) | 2009-05-27 |
| CN101440356B true CN101440356B (en) | 2011-02-16 |
Family
ID=39479499
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100320508A Pending CN101186886A (en) | 2007-12-04 | 2007-12-04 | Bacillus licheniformis 202 for highly effective preventing and curing banana wilt and application thereof |
| CN2008101830752A Expired - Fee Related CN101440356B (en) | 2007-12-04 | 2008-12-02 | Bacillus licheniformis 202 and use thereof |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100320508A Pending CN101186886A (en) | 2007-12-04 | 2007-12-04 | Bacillus licheniformis 202 for highly effective preventing and curing banana wilt and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN101186886A (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184178A (en) * | 2013-03-11 | 2013-07-03 | 大地绿源环保科技(北京)有限公司 | Bacillus licheniformis for preventing wheat scab and application thereof |
| CN106635870B (en) * | 2016-09-27 | 2019-09-13 | 安徽瑞虎肥业有限公司 | Bacillus and its application |
| CN110982725B (en) * | 2018-09-29 | 2022-08-23 | 福建省农业科学院农业生物资源研究所 | Bacillus for antagonizing fusarium wilt and promoting growth and application thereof |
| CN115125284B (en) * | 2022-06-24 | 2023-08-15 | 云南省农业科学院农业环境资源研究所 | Efficient screening method of photic-leaf vetch antagonistic bacteria |
| CN116064275B (en) * | 2022-07-06 | 2024-01-30 | 海南大学 | Spore bacteria for preventing and treating banana vascular wilt and application thereof |
-
2007
- 2007-12-04 CN CNA2007100320508A patent/CN101186886A/en active Pending
-
2008
- 2008-12-02 CN CN2008101830752A patent/CN101440356B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101440356A (en) | 2009-05-27 |
| CN101186886A (en) | 2008-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101550402B (en) | Bacillus subtilis 203 and applications thereof | |
| CN102433282B (en) | Bacillus subtilis NB12, as well as culture method and application thereof | |
| Bernal-Vicente et al. | Citrus compost and its water extract for cultivation of melon plants in greenhouse nurseries. Evaluation of nutriactive and biocontrol effects | |
| CN101486978B (en) | Bacillus licheniformis 201 and use thereof | |
| CN101575574B (en) | Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection | |
| CN101698829B (en) | Bacillus subtilis A16, preparation method thereof and application thereof | |
| CN104928201B (en) | A kind of bacillus amyloliquefaciens HN-11 and its microbial inoculum | |
| CN104164394A (en) | Antagonistic phytopathogen strain and application thereof | |
| CN102172249B (en) | Marine bacteria 2BS12 preventing and controlling banana fusarium wilt | |
| CN104651260A (en) | Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis | |
| CN104789509B (en) | Raw bacillus pumilus and its application in one plant of bark of eucommia | |
| CN103087960A (en) | Bacillus amyloliquefaciens FQS38 and application thereof | |
| CN101440356B (en) | Bacillus licheniformis 202 and use thereof | |
| CN105462881A (en) | Paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of paenibacillus polymyxa | |
| CN105238723B (en) | A kind of bacillus amyloliquefaciens and its microbial bacterial agent of prevention crop verticillium wilt | |
| CN106701623A (en) | Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus | |
| CN102533603B (en) | Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof | |
| CN108795830A (en) | A kind of angstrom of lucky series bacillus（Paenibacillus elgii）SWL-W8 and its application | |
| CN108384734A (en) | A kind of biocontrol agent and preparation method thereof of prevention tobacco rhizome class disease | |
| CN105441331A (en) | Myrothecium roridum and application thereof | |
| CN107460151A (en) | A kind of spherical lysine bacillus and its application for preventing and treating Meloidogyne incognita | |
| CN104152372B (en) | The biocontrol bacterial strain G68 of controlling plant diseases and its bacterial preparation process and application | |
| CN103205372B (en) | Paenibacillus alvei and its applications | |
| CN108559718A (en) | The myxococcus stipitatus of one plant of predacious plant pathogenetic bacteria and its application in bacterial disease biological control | |
| CN102524303A (en) | Mixture for preventing blight and verticillium wilt of cotton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110216 Termination date: 20131202 |