CN105410043A - Application of castor cake meal coarse extract in inhibition on plant pathogenic pythium - Google Patents
Application of castor cake meal coarse extract in inhibition on plant pathogenic pythium Download PDFInfo
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- CN105410043A CN105410043A CN201510994198.4A CN201510994198A CN105410043A CN 105410043 A CN105410043 A CN 105410043A CN 201510994198 A CN201510994198 A CN 201510994198A CN 105410043 A CN105410043 A CN 105410043A
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- Prior art keywords
- castor bean
- bean meal
- crude extract
- application
- suppressing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
Abstract
The invention discloses an application of a castor cake meal coarse extract in inhibition on plant pathogenic pythium. The plant pathogenic pythium includes Pythium ultimum, Pythium aphanidermatum and Pythium arrhenomanes. The castor cake meal coarse extract is prepared through the steps of weighing castor cake meal powder which is crushed and passes through a sieve in 80-120 meshes; adding distilled water, performing reflux extraction and filtering the liquid while hot and collecting a filtrate with medicine residue for later use; adding distilled water to the medicine residue, continuously performing the reflux extraction, filtering the liquid while hot and collecting a filtrate; mixing the two filtrates, and evaporating and concentrating the mixed filtrate to obtain the coarse extract. The invention not only provides an excellent novel bactericide for production but also develops a new approach of utilization of waste in castor industry.
Description
Technical field
The present invention relates to plant protection art, specifically a kind of castor bean meal crude extract is suppressing the application in pathogenic pythium spp.
Background technology
Pythium spp is subordinate to Zao Wu circle Oomycete Peronosporales pythiaceae pythium, it is universal soil fungi, saprophytic very capable, root and the seedling of 150 various vegetables fruit crops can be infected, cause damping off, the multiple diseases such as the rotten and seed decay of root-rot, rhizome, serious to agricultural production loss.Wherein, by melon and fruit corruption mould (
pythiumaphanidermatum) damping off that causes is Young Plant a kind of worldwide destructive diseases in seedling stage such as melon dish, flowers, medicinal plant, tobacco and beans, cotton, corn, Chinese sorghum.Have usually after emerging, basal part of stem place near the ground produces the dark-coloured spot of water stain shape, and then around stem expansion, hanging gradually, it is thin-line-shaped to shorten into, seedling overground part being rolled over down because losing enabling capabilities, being commonly called as disease of seizing by the throat.During high humidity, disease portion or native table can produce white cotton floccule, i.e. germ mycelia, sporangiophore and sporangium.Under the condition of benign climate, just can break out in a short time, rate of propagation is exceedingly fast, and can cause plant seedlings Large Scale Death under serious conditions, and causing is short of seedling even ruins kind, brings serious economic loss to agricultural production.
The evil frost bacterium ester sold in the market, first frost are disliked mould spirit, metalaxyl-mn-zn etc. and are belonged to toxic pesticides (low toxicity).
Summary of the invention
A kind of safe, easy degraded, noresidue, free of contamination castor bean meal crude extract is the object of the present invention is to provide to suppress the application in pathogenic pythium spp.
For achieving the above object, the invention provides following technical scheme:
Castor bean meal crude extract is suppressing the application in pathogenic pythium spp.
As the further scheme of the present invention: described pathogenic pythium spp comprises Pythium ultimum
pythiumultimum, melon and fruit corruption is mould
pythiumaphanidermatumwith strong male rotten mould
pythiumarrhenomanes.
As the further scheme of the present invention: the preparation of described castor bean meal crude extract, comprise the following steps: the castor bean meal powder taking a certain amount of pulverizing rear mistake 80 ~ 120 mesh sieve, according to castor bean meal powder: the mass volume ratio of distilled water is the ratio of 1g:8 ~ 10ml, add distilled water, refluxing extraction 1.5 ~ 2.5h, filter while hot and collect filtrate, the dregs of a decoction are continued to employ; Again according to castor bean meal powder: the mass volume ratio of distilled water is the ratio of 1g:8 ~ 10ml, adds distilled water in the dregs of a decoction, continue refluxing extraction 1.5 ~ 2.5h, filter while hot and collect filtrate; Merge twice filtrate, after evaporation and concentration, obtain brown liquid, to obtain final product.
As the further scheme of the present invention: the dry matter consumption of described castor bean meal crude extract is being greater than 0wt.%, being not more than in the number range of 20wt.%.
As the further scheme of the present invention: the dry matter consumption of described castor bean meal crude extract is 5 ~ 15wt.%.
As the further scheme of the present invention: the dry matter consumption of described castor bean meal crude extract is 5wt.%.
Compared with prior art, the invention has the beneficial effects as follows:
The invention provides castor bean meal crude extract and suppress the application in pathogenic pythium spp.Because this extract is natural materials, to Crop securify, be easy to degraded, noresidue, pollution-free.Therefore, the present invention not only provides a kind of excellent novel germicide on producing, and opens up new approach to the utilization of castor-oil plant industrial waste.
Accompanying drawing explanation
Fig. 1 be Pythium ultimum (
pythiumultimum) morphological feature (wherein, 1: mycelia expands body; 2: oogonium, spermary and egg spore);
Fig. 2 be melon and fruit corruption mould (
pythiumaphanidermatum) morphological feature (wherein, 1: sporangium and vesica; 2: oogonium, spermary and egg spore);
Fig. 3 is strong male rotten mould
(Pythiumarrhenomanes)morphological feature (wherein, 1: sporangium; 2: oogonium, spermary and egg spore);
Fig. 4 be castor bean meal crude extract to Pythium ultimum (
pythiumultimum) growth inhibition result figure; Wherein the dry matter consumption of castor bean meal crude extract is left-to-right is 0wt.%, 15wt.%, 10wt.%, 5wt.% successively;
Fig. 5 be castor bean meal crude extract to melon and fruit corruption mould (
pythiumaphanidermatum) growth inhibition result figure; Wherein the dry matter consumption of castor bean meal crude extract is from left to right 0wt.%, 15wt.%, 10wt.% successively;
Fig. 6 is that castor bean meal crude extract is to strong male rotten mould
(Pythiumarrhenomanes)growth inhibition result figure; Wherein the dry matter consumption of castor bean meal crude extract is from left to right 0wt.%, 15wt.%, 10wt.% successively.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Refer to Fig. 1 ~ 4, in the embodiment of the present invention, castor bean meal crude extract is suppressing the application in pathogenic pythium spp, comprises following step:
one, the Isolation and ldentification of pathogenic pythium spp
Gather the plant sample with the rotten mould Disease symptoms of typical case.Choose the strong intersection material of disease, with tap water 2 ~ 3 times, then be cut into the fritter of about 5mm, aqua sterilisa rinses 3 times, puts into the dull and stereotyped upper 25 DEG C of light culture of rotten mould selective medium (NARM) with aseptic filter paper after blotting surface moisture.Wait to grow after bacterium colony picking mycelia tip move on new NARM medium, carry out purifying, numbering is preserved to be identified.By purifying inoculation on CMA flat board, 25 DEG C of light culture 2 ~ 3d, to beat at colony edge with card punch and get mycelia block, and be transplanted to the dull and stereotyped central authorities of new CMA, the lawn blade of grass cutting 5mm folds up around mycelia block after sterilizing, 25 DEG C of light culture.Bacterium colony to be grown puts into 10ml sterilizing pond water (pond water: the volume ratio of distilled water is=1:2) 25 DEG C of light culture blade of grass after covering blade of grass, examines under a microscope and gather image after induced synthesis brood body.The 3 kinds of corruption obtained are mould: Pythium ultimum (
pythiumultimum), melon and fruit corruption mould (
pythiumaphanidermatum) and strong male rotten mould (
pythiumarrhenomanes) morphological feature respectively as shown in Figure 1,2 and 3.
two, the preparation of castor bean meal crude extract
Cross the castor bean meal powder 100g of 100 mesh sieves after taking pulverizing in 1L single necked round bottom flask, add 900ml distilled water, refluxing extraction 2h, filter while hot and collect filtrate.Add 900ml distilled water again in the dregs of a decoction, continue refluxing extraction 2h, filter while hot and collect filtrate.Merge 2 extracts, after evaporation and concentration, obtain brown liquid.
three, toxicity test
Toxicity test have employed and suppresses colony growth method.Castor bean meal crude extract is mixed with the series concentration gradient that dry matter consumption is respectively 20wt.%, 15wt.%, 10wt.%, 5wt.%, 0wt.%.Bacterial strain will be tried altogether respectively on CMA medium after preculture 2d, bacterium block is got at colony edge with the card punch of diameter 5mm, and bacterium block is inoculated on the CMA flat board containing serial drug concentration, cultivate under 25 DEG C of dark conditions, observe colony growth situation, each process repetition 3 times, result as shown in figures 4-6.Can be drawn by figure: dry matter consumption is the growth that the castor bean meal crude extract of 5wt.% ~ 10wt.% just can suppress 3 kinds of pythium spps completely.
four, conclusion
The castor bean meal crude extract of low concentration has very strong growth inhibition effect to plant pathogenic fungi.The plant disease control that castor bean meal crude extract causes at pythium spp has very high value.During with this material production agricultural chemicals, production technology is simple, raw material resources is abundant cheap, significantly reduces costs.And castor bean meal crude extract is natural extract, be easy to degraded in the environment, the problem of environmental pollution that the current agricultural chemicals applied in agricultural production brings can be solved.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
In addition, be to be understood that, although this specification is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification is only for clarity sake, those skilled in the art should by specification integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (6)
1. castor bean meal crude extract is suppressing the application in pathogenic pythium spp.
2. castor bean meal crude extract according to claim 1 is suppressing the application in pathogenic pythium spp, and it is characterized in that, described pathogenic pythium spp comprises Pythium ultimum
pythiumultimum, melon and fruit corruption is mould
pythiumaphanidermatumwith strong male rotten mould
pythiumarrhenomanes.
3. castor bean meal crude extract according to claim 1 is suppressing the application in pathogenic pythium spp, it is characterized in that, the preparation of described castor bean meal crude extract, comprise the following steps: the castor bean meal powder taking a certain amount of pulverizing rear mistake 80 ~ 120 mesh sieve, according to castor bean meal powder: the mass volume ratio of distilled water is the ratio of 1g:8 ~ 10ml, adds distilled water, refluxing extraction 1.5 ~ 2.5h, filter while hot and collect filtrate, the dregs of a decoction are continued to employ; Again according to castor bean meal powder: the mass volume ratio of distilled water is the ratio of 1g:8 ~ 10ml, adds distilled water in the dregs of a decoction, continue refluxing extraction 1.5 ~ 2.5h, filter while hot and collect filtrate; Merge twice filtrate, after evaporation and concentration, obtain brown liquid, to obtain final product.
4. castor bean meal crude extract according to claim 3 is suppressing the application in pathogenic pythium spp, it is characterized in that, the dry matter consumption of described castor bean meal crude extract is being greater than 0wt.%, being not more than in the number range of 20wt.%.
5. castor bean meal crude extract according to claim 4 is suppressing the application in pathogenic pythium spp, and it is characterized in that, the dry matter consumption of described castor bean meal crude extract is 5 ~ 15wt.%.
6. castor bean meal crude extract according to claim 5 is suppressing the application in pathogenic pythium spp, and it is characterized in that, the dry matter consumption of described castor bean meal crude extract is 5wt.%.
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| CN201510994198.4A CN105410043B (en) | 2015-12-24 | 2015-12-24 | Castor bean meal crude extract is inhibiting the application in pathogenic pythium spp |
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| CN105410043B CN105410043B (en) | 2018-11-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967454A (en) * | 2018-09-21 | 2018-12-11 | 北京弘纳绿源科技发展有限公司 | A kind of plant source extracts crop bactericide and its preparation process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076682A (en) * | 1993-04-30 | 1993-09-29 | 沈玉水 | Multi-effect compound fertilizer for fruit tree, flower and plant |
| JP2001114620A (en) * | 1999-10-08 | 2001-04-24 | Osaka Organic Chem Ind Ltd | Composition for controlling strawberry disease and pest |
| KR20100070265A (en) * | 2008-12-17 | 2010-06-25 | 고려바이오주식회사 | Biological germicide for preventing fruit tree disease |
-
2015
- 2015-12-24 CN CN201510994198.4A patent/CN105410043B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076682A (en) * | 1993-04-30 | 1993-09-29 | 沈玉水 | Multi-effect compound fertilizer for fruit tree, flower and plant |
| JP2001114620A (en) * | 1999-10-08 | 2001-04-24 | Osaka Organic Chem Ind Ltd | Composition for controlling strawberry disease and pest |
| KR20100070265A (en) * | 2008-12-17 | 2010-06-25 | 고려바이오주식회사 | Biological germicide for preventing fruit tree disease |
Non-Patent Citations (2)
| Title |
|---|
| S. SORO ET AL: "《ISHS Acta Horticulturae 914: III International Symposium on Tomato Diseases》", 25 November 2011 * |
| 穆莎茉莉 等: "蓖麻碱的提取与应用研究进展", 《内蒙古民族大学学报（自然科学版）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967454A (en) * | 2018-09-21 | 2018-12-11 | 北京弘纳绿源科技发展有限公司 | A kind of plant source extracts crop bactericide and its preparation process |
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