KR101170810B1 - Composition for controlling plant diseases comprising beta-sitosterol as active components and uses thereof - Google Patents

Abstract

The present invention is a beta-sitosterol (β-sitosterol) compound or Dipsacus ( Dipsacus ) comprising the same The present invention relates to a plant disease control composition containing the plant extract as an active ingredient and a plant disease control method using the same.
The control composition according to the present invention is derived from natural products and is harmless to the human body and exhibits high control activity against various plant diseases without causing environmental pollution, and thus is useful for the development of environmentally friendly natural product disinfectants and the production of high value-added organic products. Can be used.

Description

Composition for controlling plant diseases comprising beta-sitosterol as active components and uses approximately}

The present invention is a beta-sitosterol (β-sitosterol) compound or a compound containing the same ( Dipsacus asperoides or D. asper ), hare ( Dipsacus) Dipsacus , including japonicus ), and the like. The present invention relates to a plant disease control composition containing the plant extract as an active ingredient and a plant disease control method using the same.

Many phytopathogens, pests and weeds inhibit crop growth in crop cultivation, resulting in a yield reduction of 30 to 100%, depending on the crop if not controlled. Many synthetic pesticides have been developed and used to control these harmful organisms, but they have been used for plant diseases, pests, and weed control, so they are toxic to livestock, groundwater contamination, environmental pollution, ecosystem disturbance, and increased resistance to harmful organisms. Various problems are caused. As a result, eco-friendly agricultural policies are being implemented to reduce the use of synthetic pesticides by 40%, especially among OECD member countries including Korea. As part of this policy, bio-pesticides are emerging as an alternative to synthetic pesticides.

Bio pesticides include microbial pesticides and biochemical pesticides, which are environmentally friendly crop protection agents. Unlike synthetic pesticides, biopesticides can produce high value-added agricultural products because they are safe for human beings and ecosystems, and effectively control pests that are difficult to control with synthetic pesticides. Increase the production of safe crops and at the same time solve the problems caused by misuse of synthetic pesticides. In Korea, many researches on microbial pesticides have been made, but biochemical pesticides using natural products have not yet been registered, although registration regulations were established in 2005.

There are five fungicide products on the market that have been successfully commercialized using plant extracts worldwide. As of 2004, Milsana ™ is the most representative. This product is an eco-friendly disinfectant developed using the extract of Reynouria sachalinensis , and it has been shown to control powdery mildew by inducing resistance to plants rather than directly killing phytopathogens . Moreover, cinnamic aldehyde (cinnamaldehyde), which is registered as a natural product fungicides Cassia plant as an oil extracted from the seeds of (Cassia tora), as well as insecticidal activity cavity chamber William (Verticillium), A Li Estonian (Rhizoctonia), A repetition moth (Pythium Genus and Husarium Moniform ( Fusarium) moniliforme var. It has been reported to have bactericidal activity against phytopathogens such as subglutinans (canker disease). In addition, jojoba oil extracted from jojoba seeds is not only insecticidal but also has bactericidal activity against powdery mildew. Macleaya genus extract is Macleaya cordata ) extract is effective in powdery mildew, leaf spot disease of Alternaria , and leaf spot disease of Septoria . In addition, fatty acids based on oleic acid are also effective in powdery mildew.

Meanwhile, Dipsaci Radix is a genus Dipsacus belonging to the genus Dipsacaceae. asperoides Or D. asper ) and D. japonicus , which are widely used as herbal medicines, and have bactericidal, anti-type, hemostatic, analgesic and uterine growth promoting activities. It is reported. Brain function improving composition (Korean Patent No. 2004-0111336), acetylcholinesterase inhibitor composition (Korean Patent No. 2003-0031035) and Dipssacus saponin (Dipsacus saponin C) Non-narcotic central analgesic (Korean Patent No. 195-0019839), etc., as an active ingredient, has been reported.

On the other hand, phytosterols are composed of β-sitosterol (beta-sitosterol), campesterol (campesterol) and stigmasterol (Stigmasterol), and generally includes vegetable, including fruits, vegetables, nuts, grains, corn, legumes, etc. It is widely present in, and its molecular structure is very similar to cholesterol. Phytosterols block the absorption of cholesterol in the intestine and reduce cholesterol in the blood vessels. Because of this efficacy, plant sterols are widely used in food and beverage fields as well as pharmaceutical and cosmetic fields. Phytosterols are expected to grow from $ 184.6 million in 2005 to $ 395.2 million in 2012.

In relation to phytosterol, a composition for preventing and treating hyperlipidemia, atherosclerosis and liver disease (Korean Patent No. 2005-0069410), food containing phytosterol and a method for preparing the same (Korean Patent No. 2004-0098017), phytosterol or phytosterol ester Patent for a method for preparing edible oils containing (Korean Patent No. 2004-0019189), a composition for preventing and treating hyperlipidemia, arteriosclerosis and liver disease, including phytosterol, hesperidin and rutin (Korea Patent No. 2003-0101482) Filed in There are several reports of the antifungal activity of phytosterols, most of which are for beta-sitosterol. Little anti-fungal activity of camphorsterol and stigmasterol has been reported. In the case of beta-stosterol, antifungal activity is reported to be very different depending on the fungus. Kiprono, etc. (2000) are Senecio Beta-sitosterol isolated from lyratus (Asteraceae) It has been reported that the mycelial growth of Fusarium spp. Is inhibited but not the growth of Penicillum spp. (Z. Naturforsch. 55c: 485-488). F. Kawamura et al. (2004) report Malaysian Gmelia Four lignans and beta-sitosterols were isolated from arborea (Verbenaceae), four of which were lignans, or two biliary bacteria , Tramees versicolor with Fomitopsis Although it inhibited the mycelial growth of palustris , beta-sitosterol was reported to be inactive against these two basidiomycetes (Holzforschung 58: 189-192). And Lall et al. (2006) reported that Aspergillus was a beta-sitosterol isolated from Euclea natalensis (Ebenaceae). niger , A. flavus and Cladosporium It has been reported that the fungal growth of cladosporioides is inhibited, but the mycelial growth of Phytophthora is hardly inhibited (South African J. Bot. 72: 579-583). In many cases, whether natural products or synthetic compounds in vitro (in mycelial growth inhibitory effect and in vivo (in the in vitro) vivo , the control efficacy against plant diseases is quite different. Beta-sitosterol has been reported to be active against some fungi in vitro, but no in vivo antifungal activity against plant diseases has been reported. On the other hand, little is reported about the antifungal activity of camphorsterol and stigmasterol.

The present invention has been made by such a demand, the present inventors have a tomato gray mold ( Botrytis cinerea ), tomato plague ( Phytophthora Isolation and identification of antifungal actives from the gentian roots with excellent in vivo antifungal activity against infestans ) and rice blasts revealed that beta-sitosterol, campestrol and stigmasterol are effective against these plant diseases. . Accordingly, the present invention was completed by confirming that the plant sterol and the plant extract including the same exhibit excellent control against various plant diseases, and confirming that it can be used as a natural fungicide for controlling plant diseases.

In order to solve the above problems, the present invention provides an environmentally friendly plant disease control composition derived from natural products, harmless to the human body and exhibiting excellent control against various plant diseases without causing environmental pollution.

The present invention also provides a method for controlling plant diseases using the composition for controlling the above.

According to the present invention, the composition for the control of the present invention using the cheonssan root extract as an active ingredient is harmless to the human body without originating from natural products and does not cause environmental pollution, tomato blight, rice blast, rice leaf blight blight, tomato gray mold Since it exhibits control against plant fungal diseases such as diseases, it can be usefully used for the development of environmentally friendly natural product disinfectants and the production of high value-added organic products.

Figure 1 shows the antimicrobial activity in vivo against tomato gray mold and tomato late blight of phytosterols.
Figure 2 shows the mass spectrum of the isolated camphorsterol (A) and library campestrol (B).
Figure 3 shows the mass spectrum of the separated stigmasterol (A) and library stigmasterol (B).
Figure 4 shows the mass spectrum of the isolated beta-sitosterol (A) and library beta-sitosterol (B).

In order to achieve the object of the present invention, the present invention contains a plant sterol compound, such as formula (beta-sitosterol), formula (2) (campsterol) and formula (3) (stiggsterol) or a plant extract containing the same as an active ingredient To provide a composition for controlling plant diseases:

In addition, the present invention provides a method for controlling various plant diseases by treating the control composition to crops or soil.

Hereinafter, the present invention will be described in detail.

The present invention is derived from natural products are harmless to the human body and do not cause environmental pollution, plant diseases comprising the plant sterol compound of the formula (1 to 3) or a plant extract containing the same as an active ingredient exhibiting high control against various plant diseases Provided is a composition for controlling.

Preferred compounds exhibiting control activity against various plant diseases in the present invention may be a beta-sitosterol of Formula 1, camphorsterol of Formula 2, stigmasterol of Formula 3, but is not limited thereto.

Of the plant material used in the present invention, including a cloth speed (Dipsacus asper) cloth speed (Dipsacus Dipsacaceae, including asperoides ), D. japonicus , and Senecio Asteraceae belongs lyratus (Asteraceae = Compositae), Gmelia arborea the verbenaceae (Verbenaceae), Euclea belonging Ebenaceae belonging to natalensis , Discorea Plants such as Dioscoreaceae belonging to alata (Aderiye et al., 1996, Folia Microbiologica 41: 407-412) can be used.

Preferably the plant extract is a plant extract of the genus Dipsacus , more preferably Dipsacus asperoides or D. asper ) or hare ( Dipsacus) japonicus ) extract.

The plant extract according to the present invention can be prepared by extracting various plant tissues including phytosterols with an organic solvent. In the present invention, the extraction organic solvent may be used alone or mixed with a lower alcohol such as methanol, ethanol, propanol, butanol or an aqueous solution thereof, hexane, ethyl acetate, acetone, methylene chloride and the like. For example, about 5-20 L, preferably 8-12 L, lower alcohol is added to 1 kg of dried Cheondan root and leached at 20-60 ° C., preferably at room temperature for 6 hours to 3 days to lower alcohol. After obtaining the extract, it is further extracted with another organic solvent to obtain an organic solvent extract. At this time, the organic solvent that can be used may include hexane, ethyl acetate, methylene chloride, chloroform, acetone, methanol, ethanol, ethyl ether and the like. In the present invention, it is also possible to carry out the fractionation process commonly used in the art.

Phytosterols represented by Chemical Formulas 1 to 3 may be separated or purified from plant extracts or chemically synthesized. Chemical synthesis method of the phytosterol may use a general method known in the art.

Specifically, the three phytosterols having a control activity may be prepared by a method comprising the step of separating and purifying the organic solvent extract of plant tissues such as Cheondan by column chromatography. In this case, the column may be a silica gel column, a reverse phase silica gel column or a Sephadex column, and the eluent may be an organic solvent such as hexane, ethyl acetate, chloroform, methylene chloride, acetone, lower alcohol, and a mixture thereof.

The plant disease control composition of the present invention can effectively control the plant fungus, plant fungal disease is preferably tomato late blight, rice blast, rice leaf blight blight, or tomato ash fungus disease, but is not limited thereto. Methanol extracts of Cheondan Root according to the present invention, isolated from purified beta-sitosterol, camphorsterol and stigmasterol show control activity against rice blast, rice leaf blight, tomato ash mold and tomato blight (Table 1 and 2).

Thus, a composition containing beta-sitosterol, campestrol, stigmasterol, or a plant extract comprising the same as an active ingredient is innocuous to humans and does not cause environmental pollution, but is a rice blast, rice leaf blight, tomato gray mold and It can be usefully used as a natural product fungicide showing excellent control against tomato late blight.

The composition for controlling plant diseases of the present invention may include beta-sitosterol, camphorsterol, stigmasterol, or a plant extract containing the same alone, but plant fungal diseases, especially rice blast, rice leaf blight, tomato ash fungus And conventional ciryneol A, ciryneol C, 1-heptadecine-11,13-dyne-8,9,10-triol, which are known to be effective in controlling tomato late blight. heptadecene-11,13-diyne-8,9,10-triol), magnolol, honoliol, 4-methoxyhonokiol, griseofulvin and casga It may also be used with natural compounds such as kaisugamycin.

The control composition of the present invention may further include a substance contained in a commonly used insecticide or fungicide, and in addition to the active ingredient, a pharmaceutically acceptable solid carrier, liquid carrier, liquid diluent, liquefied gas diluent as an excipient , Solid diluents, or other suitable auxiliaries such as emulsifiers, dispersants or foaming agents. The composition for controlling the active ingredient and the excipients may be formulated into various formulations known in the pesticide field. For formulation, any formulation method commonly used in the pesticide field may be used.

The control composition of the present invention may preferably be formulated in a hydrated, granulated, powdered, emulsified, sprayed, smokescreen, capsule and gelled formulation and contacted through a formulation such as a donut for buoyancy of the formulation. Preferably provided as.

In order to effectively control rice blast, rice leaf blight, tomato ash fungus and tomato late blight, the composition of the present invention is a plant extract at a concentration of 10 μg / ml to 3,000 μg / ml, beta-sitosterol, camphorsterol, The stigmasterol is preferably included at a concentration of 50 μg / ml to 500 μg / ml, respectively, but it may be appropriately adjusted in consideration of the type of crop, the degree of growth, the environment of cropland, and the incidence of plant diseases.

By treating the control composition of the present invention formulated as described above to plants or areas requiring control, rice blast, rice leaf blight, tomato gray mold disease or tomato late blight can be controlled. The preferred method is to apply the control composition so as to be in direct contact with the plant pathogens to control, the most preferred is a method of mixing the soil or spraying directly to the crop.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

< Example  1> Heavenly Root Extract In plant diseases  Control activity

7 L of 80% methanol was added to 3 kg of D. asper root dried samples purchased commercially, and the extract obtained by leaching at room temperature for 24 hours was filtered using a filter paper. The methanol extract obtained therefrom was completely concentrated under reduced pressure and then refrigerated until use in experiments.

To investigate the control activities of methanol extracts against plant diseases, we conducted experiments on the control activities of plant diseases such as rice blast, rice leaf blight, tomato ash mold, tomato late blight, wheat red rust, barley flour and pepper anthrax. It was performed together. First, the methanol extract was dissolved in methanol and diluted with 250 µg / ml Tween 20 solution to prepare a final concentration of 3,000 µg / ml. At this time, the final concentration of methanol was 5%, and a solution containing 1.5 ml of methanol (final concentration 5%) and 7.5 mg of Tween 20 (final concentration 250 μg / ml) was added to 30 ml of distilled water as a negative control. Used. Methanol extracts and control samples thus prepared were spray sprayed on the foliar of plant seedlings 1 day before inoculation of the pathogen, and then dried at room temperature for 24 hours.

Rice, tomatoes, barley and wheat used in the experiment were filled with water or clay for horticulture 70% in plastic pots with a diameter of 4.5 cm, and then seeded and grown in a greenhouse at 25 ± 5 ℃ for 1 to 4 weeks. Rice blasts are seedlings of the 3-4 leaf period, Magnaporthe, the causative agent of blasts. grisea , Korea Chemical Research Institute) spore suspension (5X10 ⁵ spores / ㎖) was inoculated by spraying, wetted for one day in a 25 ℃ wet room, and then incubated for 5 days in a constant temperature room at 25 ℃ to induce the onset. Rice leaf blight is Thanatephorus which is the cause of leaf blight. cucumeris , Korea Research Institute of Chemical Technology) was incubated for 7 days in a medium (90 g of wheat bran, 15 g of chaff and 100 ml of distilled water) inoculated in 5 leaf seedlings, and then wet-treated in a 25 ° C. humidity chamber for 4 days. Incubation was induced by incubation for 4 days in a constant temperature room. Tomato late blight is 3-4 in Saratov pie causative agent of plague in the bizarre tomato seedlings inpe Afghanistan's Tora (Phytophthora infestans , Gangneung National University) After inoculation of the drift suspension from the drift sac (10 ⁵ sorghum sac / ml), the incubation was induced by incubation for 2 days in a 25 ° C. wet room and incubated in a constant temperature and humidity room at 25 ° C. for 1 day. Tomato gray mold disease is caused by Botrytis , a 3-4 leaf seedling in tomato seedlings. After treatment with the spore suspension of cinerea (Korea Research Institute of Chemical Technology) (10 ⁶ spores / ㎖), the incubation was induced by incubation for 3 days in a 20 ℃ wet room. Wheat rust is a Puccinia recondita, a causative agent of rust that is known as a biotic parasite in one-leaf seedlings. recondita (Incheon University) spores were suspended and sprayed in 250 ㎍ / ml Tween 20 solution in an amount of 0.67 g spores / l, and then treated in a humidified environment at 20 ° C for 1 day and transferred to a constant temperature room at 20 ° C for 6 days. To induce onset. Barley powdery mildew is Erysiphe , a causative agent of powdery mildew, passaged from host plants to seedlings of barley. graminis f. sp. hordei , Korea Research Institute of Chemical Technology) and spores were inoculated and incubated for 7 days in a constant temperature room at 20 ℃ to induce the onset. The area ratios of rice blast, wheat rust, and barley flour were investigated 7 days after inoculation, 8 days after inoculation for rice leaf blight, and 3 days after inoculation for tomato ash and tomato late blight.

On the other hand, for the control activity test for pepper anthracnose, 70% of horticultural soil is filled in a plastic pot with a diameter of 7.0 cm, sowing the seeds of the most pepper, grown in the greenhouse to 3 to 4 leaves and then prepared cloth Methanol extract samples of the inner roots were foliar sprayed. After spraying the sample, dried at room temperature for 24 hours and then Colletotrichum ( choletotrichum) spore suspension (4 × 10 ⁵ spores / ml) of coccodes , Korea University). After 2 days of invasion in a humid room, it was left in a constant temperature room at 25 ° C. for 1 day, and the lesion area ratio was examined after 3 days of inoculation.

The control value was calculated according to the following Equation 1 using the lesion area ratio obtained therefrom, and the control activities of the methanol extracts of the Cheondan root against the seven plant diseases are shown in Table 1 below.

sample Concentration (µg / ml) Control price (%) RCB RSB TGM TLB WLR BPM PAN Temple of Heaven Root
Methanol extract 3,000 66 5 82 73 53 0 64 RCB: rice blast; RSB: rice leaf blight; TGM: tomato gray mold;
TLB: tomato late blight; WLR: wheat rust; BPM: wheat flour; PAN: Chilli Anthrax

As shown in Table 1, the methanol extract of the Cheondan stage showed control activities against rice blast, tomato gray mold, tomato blight, wheat red rust, and pepper anthrax.

< Example  2> Isolation, Purification and Structure Determination of Controlled Active Ingredients

7 L of 80% methanol was added to 3 kg of the dried sample of Cheonndan root, and the methanol extract obtained by leaching at room temperature for 1 day was filtered using filter paper, and the filtrate was concentrated under reduced pressure. After dissolving the methanol extract of the Cheondan stage in 2 L of 70% methanol and extracting twice with the same amount of hexane, the hexane layer and the aqueous layer were concentrated under reduced pressure. The concentrated aqueous layer was again dissolved in 2 L of distilled water and extracted twice with the same amount of ethyl acetate and butanol. The three organic solvent extracts and the aqueous solution layer obtained above were concentrated under reduced pressure, and the control activities for the seven plant diseases were investigated in the same manner as in Example 1.

sample Concentration (µg / ml) Control price (%) RCB RSB TGM TLB WLR BPM PAN Hexane layer 2,000 90 5 50 81 60 0 69 Ethyl acetate layer 2,000 94 10 96 96 60 8 55 Butanol layer 2,000 66 5 7 38 43 8 60 Aqueous solution layer 2,000 50 0 21 46 13 8 0 RCB: rice blast; RSB: rice leaf blight; TGM: tomato gray mold;
TLB: tomato late blight; WLR: wheat rust; BPM: wheat flour; PAN: Chilli Anthrax

As a result, as shown in Table 2, the hexane layer and the ethyl acetate layer showed the highest overall control activity against the seven plant diseases, and the hexane layer and the ethyl acetate layer were used separately for the purification and purification of the active ingredient, respectively. It was.

In order to separate the active ingredient from the hexane layer (12.9 g), silica gel column chromatography (5X60 cm, Kaigel gel 60, 70-230) using hexane: ethyl acetate mixture (3: 1, v / v) as a developing solvent. Mesh, 500 g: Merck). The eluate obtained therefrom was separated and analyzed by thin layer chromatography (Kaygel gel 60 F254, 0.25 mm; Merck) using hexane-ethyl acetate (3: 1, v / v) as the developing solvent. According to the pattern of TLC divided into 12 fractions of F1 to F12, each fraction was dissolved in chloroform at the level of 50 mg / ㎖ and then bioassay for rice blast bacteria. The bioassay for rice blast bacterium was crushed for 10 seconds using a blender for blast bacteria cultured in potato dextrose broth (PDB) at 25 ℃, 150 rpm for 7 days. After sterilizing the turbidity of the bacterium, the inoculum was inoculated in a 1% amount of cold potato dextrose agar (PDA), shaken well, and poured into Petri dishes. After hardening of the medium, 35 μl of each fraction was dropped on a paper disc of 8 mm in diameter, and then the solvent was removed and placed on a medium inoculated with the germ. At this time, in the case of no treatment, only 35 μl of the solvent was dried and then dried. Incubation at 25 ° C. for 3 days was followed by low support. As a result, four fractions of F6 to F9 appeared to be effective, and these four fractions were combined. Sephadex LH-20 column chromatography was performed to further separate the antimicrobial substance from the active fraction (998.3 mg). Dimethyl chloride: hexane: methanol (5: 5; 1, v / v / v) was used as the elution solvent. The same column was repeated three times to separate 365.4 mg of a fraction that appeared to be a single substance in TLC. As a result of HPLC analysis to investigate the purity of the separated substance, it was found that three substances were contained, and the three substances were separated using preparative HPLC. At this time, HPLC conditions are as follows. Column: Capcell Pak C18 (20 mm × 250 mm), eluent: 100% methanol, flow rate: 10 ml / min, detection wavelength: 210 nm. Under these conditions, stigmasterol was eluted between 19.5 and 21.0 minutes, camphorsterol between 21.0 and 23.0 minutes, and beta-sitosterol between 23.0 and 25.0 minutes. As described above, beta-sitosterol (181.0 mg), camphorsterol (51.3 mg), and stigmasterol (7.7 mg) were separated purely.

GC-MS analysis was performed to determine the purity and structure of the three isolated materials. GC-MS analysis conditions are as follows. Column: DB-5 capillary column (0.25 mm X 30 m, 0.25 μm film thickness), injector temperature: 300 ° C., interface temperature: 280 ° C., column start temperature: 120 ° C. (5 min), temperature increase rate: 10 ° C. / min, column final temperature: 270 ° C. (30 min). In this analysis, camphorsterol, stigmasterol and beta-sitosterol showed single peaks at 26.48, 27.17 and 28.44 minutes, respectively, and their respective mass spectra were matched when compared to those in the library. appear. 2, 3 and 4 show the mass spectra of camphorsterol, stigmasterol and beta-sitosterol, respectively, compared to the mass spectra in the library.

<Example 3> for controlling plant diseases of the fabric speed control root extract derived active ingredients active

? Example 2 separated from cloth speed root extract in a campesterol, stigmasterol and beta-sympathetic-sitosterol in vivo (in the In order to investigate the control activities in vivo , we tested the control activities of seven plant diseases such as rice blast, rice leaf blight, tomato ash mold, tomato blight, wheat red rust, barley flour and red pepper anthrax. Was performed.

After dissolving each of the three active ingredients in acetone, the final concentration was adjusted to 250 and 500 μg / ml by adding 250 μg / ml of Tween 20 solution, and the final acetone concentration of all samples was adjusted to 10%. At this time, a solution containing 10% acetone and 250 μg / ml Tween 20 was used as a control. Two pots were used for each plant disease, and the active ingredient samples were spray-sprayed on the foliar and air-dried for 24 hours before inoculating each plant pathogen. The control activity against these seven plant diseases was investigated according to the method described in Example 1, and the results are shown in Table 3 below.

Active ingredient density
(Μg / ml) Control price (%) RCB RSB TGM TLB WLR BPM PAN Beta-sitosterol 500 25 33 50 52 0 0 0 250 25 0 30 42 0 0 0 Campus terrol 500 25 47 10 25 0 0 0 250 13 33 0 17 0 0 0 Stigmasterol 500 25 20 30 63 0 0 0 250 13 20 10 33 0 0 0 RCB: rice blast; RSB: rice leaf blight; TGM: tomato gray mold;
TLB: tomato late blight; WLR: wheat rust; BPM: wheat flour; PAN: Chilli Anthrax

As shown in Table 3, beta-sitosterol showed an effect on tomato gray mold and tomato late blight, and also showed activity against rice blast and rice leaf blight. Camphorsterol showed relatively high activity against rice leaf blight, and against tomato late blight and rice blast. The stigmasterol showed relatively high activity against tomato late blight, and against rice blast, rice leaf blight, and tomato gray mold.

Before separating each of the three substances, that is, a mixture of the three substances is called phytosterol. Usually, 40% phytosterol indicates that the content of beta-sitosterol is 40%. As a result of GC-MS analysis, the content of beta-stosterol was about 40% in the case of phytosterol separated from the stream and separated up to the preparative HPLC stage. In vivo against tomato gray mold and tomato late blight against this substance As a result of examining the antimicrobial activity, as shown in Figure 1 showed a high control activity as the concentration increases for the two plant diseases.

Claims (4)

A composition for controlling tomato gray mold disease comprising beta-sitosterol of Formula 1 or a plant extract of the genus Dipsacus comprising the same as an active ingredient:
[Formula 1]

The method of claim 1, wherein Dipsacus A composition for controlling tomato gray mold disease, wherein the genus plant is Dipsacus asperoides or D. asper or Dipsacus japonicus . According to claim 1, Dipsacus genus plant extract is characterized in that the concentration of 10 μg / ㎖ to 3,000 ㎍ / ㎖, beta-sitosterol is contained at a concentration of 50 ㎍ / ㎖ to 500 ㎍ / ㎖ Tomato ash mold control composition to the. A method for controlling tomato gray mold by treating the crop or soil with the composition of any one of claims 1 to 3.

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