KR100832746B1 - Composition for controlling plant diseases which comprises polyacetylene compounds or cirsium japonicum root extract containing them, and method for controlling plant diseases using the same - Google Patents

Abstract

A composition comprising a polyacetylene compound extracted from Cirsium sp. plant roots is provided to be harmless to human body and to induce no environmental contamination due to being derived from a natural material and show excellent controlling activity on various plant diseases. A composition for controlling plant diseases comprises a compound represented by a formula(1)(wherein R1 is OAc, Cl or OH) or an extract of Cirsium sp. plant roots including the same as an effective ingredient, wherein the Cirsium sp. is selected from the group consisting of Cirsium pendulum Fisch., Cirsium schantarense Trautv. et Meyer, Cirsium toraiense Nakai, Cirsium rhinoceros Nakai, Cirsium lineare (Thunb.) Sch-Bit., Cirsium vlassovianum Fisch., Cirsium setidens Nakai, Cirsium chanroenicum Nakai and Cirsium nipponicum (Max.) Makino. A method for controlling the plant diseases comprises a step of treating crops or soil with the composition for controlling the plant diseases.

Description

COMPOSITION FOR CONTROLLING PLANT DISEASES WHICH COMPRISES POLYACETYLENE COMPOUNDS OR CIRSIUM JAPONICUM ROOT EXTRACT CONTAINING THEM, AND METHOD FOR CONTROLLING PLANT DIS USING THE SAME}

The present invention relates to a plant composition for controlling plant diseases containing a polyacetylene compound or an extract of thistle root (Cirrus root, Cirsii Radix) including the same as an active ingredient and a plant disease control method using the same.

Many phytopathogens, pests and weeds inhibit crop growth in crop cultivation, resulting in a yield reduction of 30 to 100%, depending on the crop if not controlled. Many synthetic pesticides have been developed and used to control these harmful organisms until now, but as they are misused and misused in controlling plant diseases, pests and weeds, toxicity to humans, groundwater contamination, environmental pollution, ecosystem disturbance, and resistance to harmful organisms are increased. Various problems are caused. As a result, eco-friendly agricultural policies are being implemented to reduce the use of synthetic pesticides by 40%, especially among OECD member countries including Korea. As part of this policy, bio-pesticides are emerging as an alternative to synthetic pesticides.

Bio pesticides include microbial pesticides and biochemical pesticides, which are environmentally friendly crop protection agents. Unlike synthetic pesticides, biopesticides can produce high value-added agricultural products because they are safe for human beings and ecosystems. Increase the production of safe crops and at the same time solve the problems caused by misuse of synthetic pesticides. In Korea, many researches on microbial pesticides have been made, but biochemical pesticides using natural products have not yet been registered, although registration regulations were established in 2005.

There are five fungicide products on the market that have been successfully commercialized using plant extracts worldwide. As of 2004, Milsana ™ is the most representative. This product is an eco-friendly disinfectant developed using the extract of Reynouria sachalinensis , and it has been shown to control powdery mildew by inducing resistance to plants rather than directly killing phytopathogens . In addition, cinnamaldehyde, which is registered as a natural product disinfectant, is an oil extracted from the seeds of Cassia tora as well as insecticidal activity, Verticillium genus, Rhizoctonia genus, Pythium genus. It is reported to have bactericidal activity against phytopathogens such as Fusarium moniliforme var.subglutinans (canker disease). In addition, jojoba oil extracted from jojoba seeds is not only insecticidal but also has bactericidal activity against powdery mildew. Macleaya genus extract is Macleaya cordata extract and is effective in powdery mildew, Alternaria leaf spots, and Septoria leaf spots. In addition, fatty acids based on oleic acid are effective in powdery mildew.

Thistle ( Cirsium japonicum DC.) Is a herbaceous plant belonging to the Asteraceae family, young sprouts are edible and mature roots are medicinal. Cirsium Thistles belonging to the genus include Cirsium pendulum Fisch., Cirsium schantarense Trautv . Et Meyer, Cirsium toraiense Nakai, Cirsium rhinoceros Nakai, and Cirsium lineare ( Thunbium lineare ). Sch-Bit.), Cirsium vlassovianum Fisch., Cirsium setidens Nakai, Cirsium chanroenicum Nakai, Cirsium nipponicum (Max.) Makino, among others. The root of thistle ( Cirsium japonicum DC.) Is the Chinese medicine name Cirsii Radix and is used for rheumatoid arthritis, boil treatment and hemostasis. Regarding the use of thistle, a whitening cosmetic composition (Korean Patent No. 10-0348822), an external skin composition (Korean Patent Publication No. 10-2007-0021522) and a blood germination inhibitor composition (Korean Patent Publication No. 10-2006 -0076266) have been reported.

Thistle root is ciryneol AE, 1-heptadecine-11,13-dyne-8,9,10-triol, etc. 1-heptadecene-11,13-diyne-8,9,10-triol Polyacetylene compounds and flavonoid compounds such as ricoricidin and pectolinarin. In vitro anti-cancer activity has been reported for polyacetylene compounds (Takai et al., Phytochemistry 29: 3849-3852, 1990), but the fungicidal activity of thistle extract against phytopathogens and its polyacetylene-based compound is still unknown. None reported.

Accordingly, the present inventors have conducted research to develop a natural fungicide that is harmless to the human body using natural extracts, and found that the polyacetylene-based compound or thistle root extract containing the same exhibits excellent control against various plant diseases. The present invention was completed by finding that it can be used as a natural product fungicide for controlling plant diseases.

An object of the present invention is to provide an environmentally friendly plant disease control composition derived from natural products and harmless to the human body and exhibiting excellent control against various plant diseases without causing environmental pollution.

It is also an object of the present invention to provide a method for controlling plant diseases using the composition for controlling the above.

In order to achieve the above object, the present invention provides a composition for controlling plant diseases, containing a polyacetylene-based compound of the formula (1) or thistle root extract comprising the same:

Where

R ₁ is OAc, Cl or OH.

In addition, the present invention provides a method for controlling various plant diseases by treating the control composition to crops or soil.

Hereinafter, the present invention will be described in detail.

The present invention is derived from natural products, harmless to the human body and do not cause environmental pollution, plant disease comprising the polyacetylene-based compound of the formula (1) or thistle root extract comprising the same as the active ingredient showing high control against various plant diseases Provided is a composition for controlling.

Preferred compounds exhibiting control activity against various plant diseases in the present invention are polyacetylene-based compounds of Formula 1 as cirineneol A (ciryneol A, R ₁ = OAc), sirineol C (ciryneol C, R ₁ = Cl), 1-heptadecine-11,13-diene-8,9,10-triol (1-heptadecene-11,13-diyne-8,9,10-triol, R ₁ = OH) can be used.

Plant materials used in the present invention include the root of thistle ( Cirsium japonicum DC.) And the needle thistle ( Cirsium rhinoceros Nakai) (Lee et al . , Arch. Pharm. Res. 26: 128-131, 2003), Cirsium pendulum Fisch., Cirsium schantarense Trautv . Et Meyer, Cirsium toraiense Nakai, Cirsium lineare (Thunb.) Sch- Bit.), Cirsium vlassovianum Fisch., Cirsium setidens Nakai, Cirsium chanroenicum Nakai, Cirsium nipponicum (Max.) Makino, including Cirsium spp. Preference is given to using the roots of the various plants to which they belong.

Thistle root extract according to the present invention can be prepared by extracting thistle root or its dried matter with an organic solvent. In the present invention, the organic solvent for extraction may be C ₁ -C ₄ alcohols such as methanol, ethanol, propanol, butanol or an aqueous solution thereof, hexane, ethyl acetate, acetone, methylene chloride, or the like.

For example, to 1 kg of dried thistle root, about 5 to 20 L, preferably 8 to 12 L of C ₁ -C ₄ alcohol, are added at 20 to 60 ° C., preferably at room temperature for 6 hours to 3 days. After leaching to obtain an alcohol extract, it is further extracted with another organic solvent to obtain an organic solvent extract. At this time, usable organic solvents include hexane, ethyl acetate, methylene chloride, chloroform, acetone, ethyl ether; Or C ₁ -C ₄ alcohols such as methanol, ethanol, propanol, butanol, and the like. In the present invention, it is also possible to carry out the fractionation process commonly used in the art.

Polyacetylene-based compound represented by the formula (1), preferably sirineol A, sirineol C, 1-heptadecine-11,13-diyne-8,9,10-triol is separated and purified from thistle root extract Or chemically synthesized.

Specifically, the three polyacetylene-based compounds having a control activity may be prepared by a method comprising separating and purifying the organic solvent extract of thistle root by column chromatography. In this case, the column may be a silica gel column, reverse phase silica gel column or Sephadex column, and the eluate is hexane, ethyl acetate, chloroform, methylene chloride, acetone; And organic solvents such as methanol, ethanol, propanol, C ₁ -C ₄ alcohols such as butanol, and the like, and mixtures thereof.

In a preferred embodiment of the present invention, the dried thistle root is extracted with methanol, and the methanol extract obtained by concentrating under reduced pressure is dissolved in distilled water, and then the extract obtained by extracting with an organic solvent such as ethyl acetate is concentrated under reduced pressure, and then the concentrate is repeatedly concentrated. Purified by Phosphorous Silica Gel Column Chromatography and Sephadex LH-20 Column Chromatography to purely separate cyrineol A, sirineol C and 1-heptadecine-11,13-dyne-8,9,10-triol .

Methanol extracts of thistle roots according to the present invention, sirineol A, sirineol C and 1-heptadecine-11,13-dyne-8,9,10-triol isolated and purified from the rice blast, rice leaf Control activity against blight blight, tomato blight, wheat rust, wheat flour and pepper anthrax (see Tables 1 and 3), Pythium ultimum , Fusarium oxysporum , Alterna It also shows a high spore germination inhibitory effect on phytopathogens such as Alternaria alternata (see Table 4).

Therefore, the composition containing cylinol A, cylinol C, 1-heptadecine-11,13-diyne-8,9,10-triol or thistle root extract containing the same as an active ingredient is harmless to the human body and the environment. Excellent control against rice blast, rice leaf blight, tomato gray mold, tomato blight, wheat red rust, barley flour, red pepper anthracnose, pismos mozzarella, fusidium wilting and alternaria without causing contamination It can be usefully used as a natural product disinfectant.

The control composition of the present invention may further include a substance contained in a commonly used insecticide or fungicide, and in addition to the active ingredient, a pharmaceutically acceptable solid carrier, liquid carrier, liquid diluent, liquefied gas diluent, Solid diluents, or other suitable auxiliaries such as emulsifiers, dispersants or foaming agents, may further be included. The composition for controlling the active ingredient and the excipients may be formulated into various formulations known in the pesticide field, and any formulation method commonly used in the pesticide field may be used for formulation.

The control composition of the present invention may preferably be formulated in a hydrated, granulated, powdered, emulsified, sprayed, smokescreen, capsule and gelled formulation and contacted through a formulation such as a donut for buoyancy of the formulation. Preferably provided as.

The control composition of the present invention to effectively control rice blast, rice leaf blight, tomato gray mold, tomato blight, wheat red rust, barley flour, pepper anthracnose, pisium mozzarella, fusidia rot or alternaria disease Milk thistle root extract at a concentration of 100 μg / ml to 3,000 μg / ml, and 50 μg / g of sirinol A, sirinol C, and 1-heptadecine-11,13-dyne-8,9,10-triol, respectively. It is preferable to include in a concentration of ㎖ ~ 500 ㎍ / ㎖, this can be appropriately adjusted in consideration of the type of crop, the degree of growth, cropland environment, the degree of development of plant diseases.

By treating the control composition of the present invention formulated as described above to plants or areas that require control, rice blast, rice leaf blight, tomato gray mold, tomato blight, wheat red rust, barley powdery mildew, pepper anthrax, fish You can control Mozolox disease, Fusidium wilting disease or Alternaria disease. The preferred method is to apply the control composition so as to be in direct contact with the plant pathogens to control, the most preferred is a method of mixing the soil or spraying directly to the crop.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Control Activity of the Thistle Root Extract on Plant Disease

To the dried root sample of Cirsium japonicum , commercially purchased 7 L of methanol was added and the extract obtained by leaching at room temperature for 24 hours was filtered using a filter paper. The methanol extract obtained therefrom was completely concentrated under reduced pressure and then refrigerated until use in experiments.

To investigate the control activities of methanol extracts against plant diseases, we conducted experiments on the control activities of representative plant diseases rice blast, rice leaf blight, tomato ash mold, tomato late blight, wheat rust disease, barley flour and pepper anthrax. It was performed together. First, the methanol extract was dissolved in methanol and diluted with 250 µg / ml Tween 20 solution to prepare a final concentration of 3,000 µg / ml. At this time, the final concentration of methanol was 5%, and a solution containing 1.5 ml of methanol (final concentration 5%) and 7.5 mg of Tween 20 (final concentration 250 μg / ml) was added to 30 ml of distilled water as a negative control. Used. Methanol extracts and control samples thus prepared were spray sprayed on the foliar of plant seedlings 1 day before inoculation of the pathogen, and then dried at room temperature for 24 hours.

Rice, tomatoes, barley and wheat used in the experiment were filled with water or soil for horticulture in a plastic pot of 4.5 cm in diameter about 70%, and then seeded and grown in a greenhouse at 25 ± 5 ℃ for 1 to 4 weeks. Rice blasts are sprayed with 3-4 leaf seedlings and sprayed with a spore suspension (5 × 10 ⁵ spores / ml) of Magnaporthe grisea (Korea Research Institute of Chemical Research), which is the causative agent of blasting disease, After the wet treatment, the incubation was induced by incubation for 5 days in a constant temperature room at 25 ℃. Rice foliar blight is cultured by culturing 7 days of cultivation of Thanatephorus cucumeris , a causative agent of blight blight, in medium (90 g of wheat bran, 15 g of chaff and 100 ml of distilled water). The seedlings were inoculated to seedlings, wet-treated in a wet chamber at 25 ° C. for 4 days, and then cultured in a constant temperature room at 25 ° C. for 4 days to induce the onset. The tomato late blight is caused by Phytophthora infestans (Gangneung University) After inoculation of the drift suspension from the drift sac (10 ⁵ sorghum sac / ml), the incubation was induced by incubation for 2 days in a 25 ° C. wet room and incubated in a constant temperature and humidity room at 25 ° C. for 1 day. Tomato gray mold disease was treated with spore suspension (10 ⁶ spores / ml) of Botrytis cinerea (Korea Research Institute of Chemical Technology), which is a causative agent of gray mold disease, in tomato seedlings of 3-4 leaves. In fact, 3 days incubation was induced to induce. Wheat red rust sprays spores of Puccinia recondita (University of Incheon), the causative agent of rust, known as biolactic bacteria, in a single-leaf seedling, suspended in 250 µg / ml Tween 20 solution with 0.67 g spores / ℓ. After treatment for one day in a 20 ℃ humid room and transferred to a 20 ℃ constant temperature room was incubated for 6 days to induce the onset. Barley powdery mildew is inoculated with spores of Erysiphe graminis f. Sp. Hordei (Korea Research Institute of Chemical Technology), which is the causative agent of powdery mildew, passaged from host plants, in barley seedlings. Incubation was induced by incubation for 7 days in a constant temperature room. The area ratios of rice blast, wheat rust, and barley flour were investigated 7 days after inoculation, 8 days after inoculation for rice leaf blight, and 3 days after inoculation for tomato ash and tomato late blight.

On the other hand, for the control activity test for pepper anthracnose, 70% of horticultural soil is filled in a plastic pot with a diameter of 7.0 ㎝, sowing the seeds of the most peppers, growing them up to 3 to 4 leaves in a greenhouse, and then preparing thistle Methanol extract samples of roots were sprayed with foliar surfaces. After spraying the sample was dried at room temperature for 24 hours, and then spray inoculated with a spore suspension (4 × 10 ⁵ spores / ml) of the pepper anthrax pathogen , Colletotrichum coccodes (Korea University). After 2 days of invasion in a humid room, it was left in a constant temperature room at 25 ° C. for 1 day, and the lesion area ratio was examined after 3 days of inoculation.

The control value was calculated according to the following Equation 1 using the lesion area ratio obtained therefrom, and the control activity of the methanol extract of the thistle root against the seven plant diseases is shown in Table 1 below.

As shown in Table 1, the methanol extract of thistle root showed very high control against rice blast, rice leaf blight, tomato blight, wheat red rust, and pepper anthrax, and medium for tomato gray mold and barley powdery mildew. It showed a degree of activity.

Example 2 Isolation and Purification of the Controlled Active Ingredient

7 L of methanol was added to 3 kg of thistle root dried samples, and the methanol extract obtained by leaching at room temperature for 1 day was filtered using filter paper, and the filtrate was concentrated under reduced pressure. The methanol extract of the thistle root was dissolved in 2 L of 70% methanol, extracted twice with the same amount of hexane, and the hexane layer and the aqueous layer were concentrated under reduced pressure. The concentrated aqueous layer was dissolved in 2 L of distilled water and extracted twice with the same amount of ethylacetate and butanol. The three organic solvent extracts and the aqueous solution layer obtained above were concentrated under reduced pressure, and the control activities for the seven plant diseases were investigated in the same manner as in Example 1.

As a result, as shown in Table 2, the hexane layer and the ethyl acetate layer showed the highest overall control activity against the seven plant diseases, and the hexane layer and the ethyl acetate layer were combined to separate and purify the active ingredient. Used.

In order to separate the active ingredient from the hexane layer + ethyl acetate layer (30 g), a portion of the extract (7.0 g) was mixed with hexane: ethyl acetate mixture (5: 1, 2: 1) and chloroform: methanol. Silica gel column chromatography (5 × 60 cm, Kaigel gel 60, 70-230 mesh, 400 g: Merck) was used as a developing solvent (9: 1) as a developing solvent. The eluate obtained therefrom was separated and analyzed by thin layer chromatography (Kaygel gel 60 F254, 0.25 mm; Merck) using a mixture of chloroform: methanol (99: 1 or 95: 5, v / v) as the developing solvent. . Divide into seven fractions of F1 to F7 according to the pattern of TLC (Rf value in a mixture of chloroform: methanol (95: 5); F1 => 0.53, F2 = 0.36-0.53, F3 = 0.32-0.49, F4 = 0.21-0.32, F5 = 0.15-0.32, F6 = 0.1-0.19, F7 = <0.1), each fraction was dissolved in methanol and then diluted to a concentration of 2,000 µg / ml with a 250 µg / ml solution, In the same manner as in Example 1, their control activities against seven plant diseases were investigated.

As a result, the F2, F5 and F6 fractions showed high control activity. The F2 fraction (217 mg) appeared as a nearly single substance on thin layer chromatography, and Sephadex-LH20 column (Sigma) chromatography using methylene chloride: hexane: methanol (5: 5: 1, v / v / v) Substance 1 (110 mg) was isolated purely via chromatography. Material 2 (320 mg) was isolated purely via Sephadex-LH20 column chromatography using F5 fraction (950 mg) methylenechloride: hexane: methanol (5: 5: 1, v / v / v). Similarly to the F2 fraction and the F5 fraction, the F6 fraction (1.23 g) was also subjected to Sephadex-LH20 column chromatography under the same conditions to obtain approximately 500 mg of pure substance, followed by recrystallization with acetone, substance 3 (400 mg). Was separated purely.

Example 3 Structure Determination of a Control Active Ingredient

Three control active ingredients separated in Example 2 were analyzed using a mass spectrometer. Mass spectrometry of the material 1 using an electrospray ionization mode showed [M + Na] ⁺ ions at m / z 319.4, which was estimated to have a molecular weight of 296. ¹ H and ¹³ C-nuclear magnetic resonance analysis revealed that substance 1 is consistent with sirineol C reported by Takaishi et al. (Phytochemistry 29: 3849-3852, 1990). Mass 2 was analyzed by Fast Atomic Bombardment Mode, which showed [M + K] ⁺ ions at m / z 359, and [M + H] ⁺ ions at 321. Was estimated. As a result of nuclear magnetic resonance analysis, it was identified as sirineol A reported by Takaishi et al. (1990). Material 3 had a molecular weight of 278 due to electrospray ionization mode mass spectrometry [M + Na] + ions at m / z 301, and the results of nuclear magnetic resonance analysis reported by Takaishi et al. (1990). It was identified as 1-heptadecin-11,13-diyne-8,9,10-triol.

<Example 4> Control activity against plant diseases of thistle root extract-derived active ingredient

Example 2 and isolated from milk thistle root extract 3, the identified sirine all A, C of sirine ol and 1-heptanoic decyne -11,13- die of -8,9,10- triol in vivo (in vivo) In order to investigate the control activity, a control activity test was performed on a total of seven plant diseases including rice blast, rice leaf blight, tomato ash fungus, tomato blight, wheat red rust, barley flour and red pepper anthrax. .

After dissolving each of the three active ingredients in acetone, 250 μg / ml Tween 20 solution was added to adjust the final concentrations to 125, 250 and 500 μg / ml and the final acetone concentrations of all samples were adjusted to 10%. At this time, a solution containing 10% acetone and 250 μg / ml Tween 20 was used as a control. Two pots were used for each plant disease, and the active ingredient samples were spray-sprayed on the foliar and air-dried for 24 hours before inoculating each plant pathogen. The control activity against these seven plant diseases was investigated according to the method described in Example 1, and the results are shown in Table 3 below.

As shown in Table 3, all three substances isolated showed high antimicrobial activity against rice blast, rice leaf blight, tomato blight, wheat red rust, pepper anthrax. In the case of tomato gray mold, all three substances showed moderate activity, and in case of barley powdery mildew, cinelineol C showed a high effect, but cinelineol A and 1-heptadecine-11,13-dyne-8 , 9,10-triol showed moderate activity.

<Example 5> Effect of thistle root extract-derived active ingredient on germination and growth of various pathogens

The effects of spore germination and growth on various phytopathogenic fungi of the materials isolated and identified in Examples 2 and 3 were investigated. Each material was dissolved in dimethyl sulfoxide (DMSO) at a level of 10, 3.3, 1.1, 0.37, 0.123 mg / ml and used in the experiment. Alternaria alternata ( Korea Research Institute of Chemical Technology), Magnaporthe grisea (Korea Research Institute of Chemical Technology), Thanatephorus cucumeris (Korea Research Institute of Chemical Technology), Colletotrichum coccodes , Korea University), Colletotrichum acutatum (Chungbuk National University), Colletotrichum gloeosporioides (Korea Research Institute of Chemical Technology), Fusarium oxysporum (Korea Research Institute of Chemical Technology) ), Spores produced in solid medium for 8 fungi, including Pythium ultimum (Korea Research Institute of Chemical Technology) and Botrytis cinerea (Korea Research Institute of Chemical Research), potato dextrose liquid medium (PDB; harvested with potato dextrose broth), adjusted to 1 × 10 ⁵ spores / ml and used as inoculum. In the case of Rhizoctonia solani , spores do not form spores, and the cultured medium that has been cultured in potato dextrose liquid medium for 7 seconds in a culture medium that has been ground for 10 seconds is added to potato dextrose liquid medium. It was used as. After dispensing 1 μl of the solution into each well of a 96-well plate, 100 μl of the inoculum was dispensed and incubated at 25 ° C. for 4 days, and then the minimum inhibitory concentration was investigated.

As can be seen from the results of Table 4, all of the substances isolated and identified in Examples 2 and 3 were found to have a spore germination or growth inhibitory effect against phytopathogenic fungi, in particular Magnapolese greece, colletto Inhibition of spore germination was very high against Tricom cocodes, Colletotricom akutatum and Pisium Ultimum. For spore germination of Botrytis cineraria, the effects of cyrineol A and C were relatively high.

As described above, the control composition of the present invention using thistle root extract as an active ingredient is derived from natural products and harmless to the human body without causing environmental pollution, rice blast, rice leaf blight blight, tomato ash fungus, It has high control against tomato late blight, wheat rust, wheat flour and pepper anthrax, so it can be useful for the development of eco-friendly natural product disinfectant and production of high value-added organic products.

Claims (7)

Composition for the control of plant diseases containing the compound of formula 1 or Cirsium genus plant root extract comprising the same as an active ingredient: [Formula 1]

Where R ₁ is OAc, Cl or OH. The method of claim 1, Plant disease control, characterized in that the plant disease is rice blast, rice leaf blight blight, tomato gray mold, tomato blight, wheat red rust, barley flour, pepper anthrax, psium mozzarella, fusidium wilting disease or alternaria disease Composition. The method of claim 1, The staking Titanium (Cirsium) in plant root extract is C ₁ -C ₄ alcohols, and their aqueous solution, characterized in hexane, ethyl acetate, methylene chloride, and that is manufactured by using a solvent selected from the group consisting of a mixture thereof, Plant Disease Control composition. The method of claim 1, The plant root extract of the genus Cirsium ( Cyrsium ) is characterized in that it comprises a concentration of 100 ㎍ / ㎖ to 3,000 ㎍ / ㎖, plant disease control composition. The method of claim 1, The compound of formula 1 is characterized in that it comprises a concentration of 50 μg / ㎖ to 1,000 ㎍ / ㎖, plant disease control composition. The method of claim 1, Plants of the genus Cirsium are Cirsium pendulum Fisch., Cirsium schantarense Trautv . Et Meyer, Cirsium toraiense Nakai, Cirsium rhinoceros Nakai, Cirsium lineare (Thunb.) Sch-Bit.), Cirsium vlassovianum Fisch., Cirsium setidens Nakai, Cirsium chanroenicum Nakai and Cirsium nipponicum (Max.) Makino. Characterized in that the plant disease control composition, characterized in that selected from. A method of controlling plant diseases, comprising treating the plant or plant composition for controlling plants according to claim 1 to crops or soil.