KR100832745B1 - Composition for controlling plant diseases which comprises lignan compounds, resorcinol compounds or nutmeg extract and method for controlling plant disease using same - Google Patents

Abstract

The present invention relates to a plant disease control composition comprising a lignan compound, a resorcinol compound or a nutmeg extract comprising the same as an active ingredient, and a plant disease control method using the same. The control composition according to the present invention is derived from natural products and is harmless to the human body and exhibits high control activity against various plant diseases without causing environmental pollution, and thus is useful for the development of environmentally friendly natural product disinfectants and the production of high value-added organic products. Can be used.

Description

COMPOSITION FOR CONTROLLING PLANT DISEASES WHICH COMPRISES LIGNAN COMPOUNDS, RESORCINOL COMPOUNDS OR NUTMEG EXTRACT AND METHOD FOR CONTROLLING PLANT DISEASE USING SAME}

1 is a diagram illustrating a process of separating and purifying the active ingredient from a nutmeg according to Example 2 of the present invention.

The present invention relates to a plant disease control composition comprising a lignan compound, a resorcinol compound or a nutmeg extract comprising the same as an active ingredient, and a plant disease control method using the same.

Intensive complexation of cultivated crops and year-round cultivation systems intensify the incidence and extent of disease and pests, resulting in a yield reduction of 30 to 100%, depending on the crop. Synthetic pesticides have played an important role in freeing humanity from hunger through food security worldwide, but they are being misused in controlling diseases, pests and weeds. It is caused.

Biopesticides are environmentally friendly crop protection agents, unlike synthetic pesticides, which are safe for human farming and ecosystems, which can produce high value-added agricultural products. It can solve the problems caused by misuse. Recently, researches are being actively conducted around the world to develop environmentally friendly biopesticides that effectively protect crops while minimizing their impact on the environment and other biological systems. As part of this research, various microbial pesticides, biochemical pesticides, natural product pesticides and signal chemicals have been developed and marketed in the US and Europe. A total of 19 microbial pesticides are registered in Korea, including 5 fungicides and 14 pesticides, of which 12 are imported microorganisms. In the case of biochemical pesticides using plant extracts or plant-derived substances, registration regulations were established in 2005, but there are no registered biochemical pesticides.

Nutmeg, Myristica fragrans Houtt. It is called nutmeg (Myristicae Semen), and peeled and dried meat skin is called mace. Nutmeg contains essential oils and solid fats and is used as a fragrance for stomach, sensation, spice, hair dye. In addition, nutmeg has similar properties to hallucinogens, so if you take a lot of hallucinations and phantoms increase. In Korea, processed foods using nutmeg extract (Korea Patent Registration No. 387994), skin compositions (Korea Patent Publication No. 2002-91487), oral care compositions (Korea Patent Publication No. 2006-24369), and algae activity (Korea Patent Registration) No. 517519) is disclosed. Moreover, the invention about anticancer activity (Korean Patent Publication No. 1999-34285) of dihydroguaiaretic acid isolate | separated from nutmeg is also disclosed.

Nutmeg contains the lignan compound and the resorcinol compound malabaricone as an active ingredient, and for the lignan compound anticancer activity and antioxidant activity (Filleur et al., Planta Med. 67: 700-704, 2001), anti Bacterial activity (Nakatani et al., Phytochemistry 27: 3127-3129, 1988), changes in liver enzyme activity (Woo et al., Phytochemistry 26: 1542-1543, 1987), antiviral activity (Hwu et al ., J. Med. Chem . 41 : 2994-3000, 1998), DNA polymerase I and II inhibitory activity (Li et al . , Biol. Pharm. Bull . 27: 1147-1150, 2004). In addition, antioxidant activity (Birija et al ., J. Agric. Food. Chem . 53: 6912-6918, 2005), and arg-gingipain inhibitory activity, Shinohara et al ., Biosci. Biotechnol. Biochem . 63: 1475-1477, 1999), antibacterial activity (Sinohara et al ., Biosci. Biotechnol. Biochem . 63: 1475-1477, 1999; Orabi et al ., J. Nat. Prod . 54: 856-859, 1991) and antifungal activity against Candida albicans (Orabi et al . , J. Nat. Prod . 54: 856-859, 1991). However, the antimicrobial activity of nutmeg extracts and their active ingredients, lignans and resorcinol compounds, against filamentous fungi, including phytopathogenic fungi, has not been reported.

As mentioned above, there is a need for the development of biopesticides that are more effective than ever, due to various problems of synthetic pesticides and demand for organic products. Therefore, the present inventors have conducted research to develop an environmentally friendly natural product fungicide that is harmless to the human body using natural extracts and materials. As a result, the lignan compound, resorcinol compound, or nutmeg extract containing the same is very high for various plant diseases. The present invention was completed by finding that it exhibits control activity and confirming that it can be used as a natural fungicide for controlling plant diseases.

An object of the present invention is an environmentally friendly plant which is derived from natural products and shows excellent control against various plant diseases such as rice blast, tomato late blight, wheat red rust, barley flour and red pepper anthrax without harming the human body and causing environmental pollution. It is providing the composition for bottle control.

It is also an object of the present invention to provide a method for controlling plant diseases using the composition for controlling the above.

In order to achieve the above object, the present invention provides to the lignan compound, nektan gave -B, resorcinol when phenolic compound or a composition for controlling plant diseases comprising a nutmeg extract containing them of the formula (III) of formula 2 of the formula :

Where

R ₁ and R ₂ are each independently OMe or OH, or are linked together to form —OCH ₂ O—.

Where

R ₁ and R ₂ are each independently H or OH.

In addition, the present invention provides a method for controlling various plant diseases by treating the control composition to crops or soil.

Hereinafter, the present invention will be described in detail.

The present invention is derived from natural products, harmless to the human body and do not cause environmental pollution, the lignan compound of formula (1 ), nectandrin-B of formula (2 ), resorcinol system of formula (3) exhibiting high control against various plant diseases It provides a compound or a composition for controlling plant diseases comprising the nutmeg extract containing the same as an active ingredient.

Preferred compounds exhibiting antifungal activity against a variety of plant diseases in the present invention is a lignan compound of the formula (1) erythromycin-Ost bay lignans -6 (erythro -austrobailignan-6; R 1 -R 2 = -OCH 2 O- ) And meso- dihydroguaiaretic acid (R ₁ = OMe, R ₂ = OH); Nectandrin-B of Formula 2, which is a lignan compound; And when dry as resorcinol phenolic compound of the formula (3) barikon _{-A (malabaricone-A; R 1} = R 2 = H), do barikon _{-B (R 1 = H, R} 2 = OH) and do barikon -C (R ₁ = R ₂ = OH) can be used.

Plant materials used in the present invention include nutmeg ( Myristica fragrans Houtt.) And M. argentea ( M. argentea ) known to produce the active ingredients of the control formula (1) to ( 3 ); It is preferable to use seeds or seedlings of about 100 plants belonging to Myristica , including Papuya-type nutmeg.

The nutmeg extract according to the present invention can be prepared by extracting seeds of nutmeg or dried products thereof, and nutmeg (mace), that is, the seed of nutmeg with an organic solvent. As the organic solvent for extraction in the present invention, lower alcohols such as methanol, aqueous methanol solution, ethanol and aqueous ethanol solution, hexane, ethyl acetate, acetone, methylene chloride, etc. may be used alone or in combination.

For example, about 5 to 20 L, preferably 8 to 12 L, of lower alcohol is added to 1 kg of dried nutmeg, and the lower alcohol extract is leached at 20 to 60 ° C, preferably at room temperature for 6 hours to 3 days. After obtaining, it is further extracted with another organic solvent to obtain an organic solvent extract. In the present invention, it is also possible to carry out the fractionation process commonly used in the art.

Lignan-based compounds represented by the above formulas (1) and ( 2 ), preferably erythro-ostrobayignan-6, meso-dihydroguaiaretic acid and nectandrin-B, or a resorcinol-based compound represented by formula (3 ), Preferably Malabaricone-A, B and C can be isolated, purified or chemically synthesized from the nutmeg extract.

Specifically, the lignan compound and the resorcinol compound having the six control activities can be prepared by a method comprising separating and purifying the organic solvent extract of nutmeg by column chromatography ( FIG. 1). Reference). In this case, the usable column may be a silica gel column, a reverse phase silica gel column or a Sephadex column, and the eluent may be an organic solvent such as hexane, ethyl acetate, chloroform, methylene chloride, acetone, lower alcohol, and a mixture thereof. .

In a preferred embodiment of the present invention, the dried nutmeg extracted with methanol and concentrated under reduced pressure to dissolve the methanol extract obtained by distilled water, and then extracted with an organic solvent such as ethyl acetate and concentrated under reduced pressure, the concentrated solution is repeated Purification was performed by silica gel column chromatography and Sephadex LH-20 column chromatography to purify erythro-ostroglyignan-6, meso-dihydroguaiaretic acid, nectanrin-B, malariconic-A, B and C pure water. To separate.

Methanol Extract of Nutmeg according to the Present Invention, Control Activity of Erythro-Ostrobeirignan-6, Meso-Dihydroguaiaretic Acid, Nectandrin-B, Malabaricone-A, B and C As a result of the investigation, methanol extract of nutmeg was used against rice blast, tomato late blight, wheat rust, barley flour and red pepper anthrax; Erythro-austrobayignan-6 is used against rice blast, wheat rust and barley flour; Meso-dihydroguaiaretic acid is used against rice blast, rice leaf blight and wheat rust; Nectandrin-B showed control against rice blast, rice leaf blight, tomato blight and wheat rust. And Malabaricon-A and B are against rice blast, wheat rust and pepper anthrax; Malabaricon-C showed control activity against rice blast, tomato late blight, wheat rust and pepper anthrax (see Tables 1 and 3 ).

Erythro-Ostrobayrignan-6, meso-dihydroguaiaretic acid, nectandrin-B, Malabaricon-A, B and C or nutmeg extracts comprising the same according to the present invention exhibiting such characteristics are It is harmless and has high control activity against various plant diseases without causing environmental pollution, so it can be usefully used as natural product disinfectant for rice blast, rice leaf blight, tomato blight, wheat rust disease, barley flour and pepper anthrax. .

Therefore, the composition for the control of rice blast, rice leaf blight, tomato blight, wheat red rust, barley flour and red pepper anthracnose, comprising the lignan compound, resorcinol compound or nutmeg extract containing the same as an active ingredient, is also within the scope of the present invention. Included in

The control composition of the present invention may further include a substance contained in a commonly used insecticide or fungicide, and in addition to the active ingredient, a pharmaceutically acceptable solid carrier, liquid carrier, liquid diluent, liquefied gas diluent, Solid diluents, or other suitable auxiliaries such as emulsifiers, dispersants or foaming agents, may further be included. The control composition of the present invention may be used by formulating an agricultural composition by mixing the active ingredient and the excipient, and all methods commonly used in the art may be applied as a method of formulating an agricultural composition.

The control composition of the present invention is preferably a formulation of a hydrating agent, granule, powder, emulsion, spray, smokescreen, capsule and gel, and is preferably provided as a contact through a formulation such as donut for buoyancy of the composition. Do.

In order to effectively control rice blast, rice leaf blight, tomato blight, wheat red rust, barley flour and red pepper anthrax, the nutmeg extract has a concentration of 100 µg / ml to 3,000 µg / ml. Ostrobeirignan-6, meso-dihydroguaiaretic acid, nectandrin-B, Malabaricon-A, B and C are preferably included at a concentration of 100 μg / ml to 1,000 μg / ml, respectively. This can be appropriately adjusted in consideration of the type of crop, the degree of growth, the cultivated land environment, the extent of the development of plant diseases.

By treating the control composition of the present invention formulated as described above to plants or areas that need control, rice blast, rice leaf blight, tomato blight, wheat rust, barley flour or pepper anthrax can be controlled. The preferred method is to apply the control composition so as to be in direct contact with the plant pathogens to control, the most preferred is a method of mixing the soil or spraying directly to the crop.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Control Activities of Nutmeg Extracts against Plant Disease

1 l of methanol was added per 100 g of the dried seeds of Myristica fragrans , and the extract obtained by leaching at room temperature for 24 hours was filtered using a filter paper. The methanol extract thus obtained was concentrated under reduced pressure completely and refrigerated until use in the experiment.

To investigate the control activities of methanol extracts against plant diseases, we conducted experiments on the control activities of representative plant diseases rice blast, rice leaf blight, tomato ash mold, tomato late blight, wheat rust disease, barley flour and pepper anthrax. It was performed together. First, the methanol extract was dissolved in methanol and diluted with 250 µg / ml Tween 20 solution to prepare a final concentration of 3,000 µg / ml. At this time, the final concentration of methanol was 5%, and a solution containing 1.5 ml of methanol (final concentration 5%) and 7.5 mg of Tween 20 (final concentration 250 μg / ml) was added to 30 ml of distilled water as a negative control. Used. Methanol extracts and control samples thus prepared were spray sprayed on the foliar of plant seedlings 1 day before inoculation of the pathogen, and then dried at room temperature for 24 hours.

Rice, tomatoes, barley and wheat used in the experiment were filled with water or clay for horticulture 70% in plastic pots with a diameter of 4.5 cm, and then seeded and grown in a greenhouse at 25 ± 5 ℃ for 1 to 4 weeks. Rice blasts are sprayed with 3-4 leaf seedlings and sprayed with a spore suspension (5 × 10 ⁵ spores / ml) of Magnaporthe grisea (Korea Research Institute of Chemical Research), which is the causative agent of blasting disease, After the wet treatment, the incubation was induced by incubation for 5 days in a constant temperature room at 25 ℃. Rice foliar blight is cultured by culturing 7 days of cultivation of Thanatephorus cucumeris , a causative agent of blight blight, in medium (90 g of wheat bran, 15 g of chaff and 100 ml of distilled water). The seedlings were inoculated to seedlings, wet-treated in a wet chamber at 25 ° C. for 4 days, and then cultured in a constant temperature room at 25 ° C. for 4 days to induce the onset. The tomato late blight is caused by Phytophthora infestans (Gangneung University) After inoculation of the influenza suspension extracted from the incubator sacs (10 ⁵ incubator sac / ml), the incubation was induced by incubation for two days in a humidified environment at 25 ° C. and incubated in a constant temperature and humidity room at 25 ° C. for two days. Tomato gray mold disease was treated with spore suspension (10 ⁶ spores / ml) of Botrytis cinerea (Korea Research Institute of Chemical Technology), which is the causative agent of gray mold disease, in tomato seedlings of 3-4 leaves. In fact, 3 days incubation was induced to induce. Wheat red rust sprays spores of Puccinia recondita (University of Incheon), the causative agent of rust, known as biolactic bacteria, in a single-leaf seedling, suspended in 250 µg / ml Tween 20 solution with 0.67 g spores / ℓ. After treatment for one day in a 20 ℃ humid room and transferred to a 20 ℃ constant temperature room was incubated for 6 days to induce the onset. Barley powdery mildew is inoculated with spores of Erysiphe graminis f. Sp. Hordei (Korea Research Institute of Chemical Technology), which is the causative agent of powdery mildew, passaged from host plants, in barley seedlings. Incubation was induced by incubation for 7 days in a constant temperature room. The area ratios of rice blast, wheat rust, and barley flour were 7 days after inoculation, 8 days after inoculation for rice leaf blight, and 3 and 4 days after inoculation for tomato ash and tomato blight, respectively.

On the other hand, for the control activity test for pepper anthracnose disease, 70% of horticultural soils are filled in a plastic pot with a diameter of 7.0 ㎝, sowing the seeds of the lowest pepper, growing them in the greenhouse to 5 to 6 leaves and then prepared above. Methanol extract samples of nutmeg were foliarly sprayed. After spraying the sample was dried at room temperature for 24 hours, and then spray inoculated with a spore suspension (1 × 10 ⁶ spores / ml) of the red pepper Anthrax bacterium Colletotrichum coccodes (Korea University). After 2 days of invasion in a humid room, it was left in a constant temperature room at 25 ° C. for 1 day, and the lesion area ratio was examined after 3 days of inoculation.

The control value was calculated according to the following Equation 1 using the disease area ratio obtained therefrom, and the control activities of the methanol extract of nutmeg against the seven plant diseases are shown in Table 1 below.

As shown in Table 1 , the methanol extract of nutmeg showed very high control against rice blast, tomato late blight, wheat red rust, barley flour and red pepper anthrax.

Example 2 Isolation and Purification of the Controlled Active Ingredient

1 L of methanol was added to 100 g of the dried sample of nutmeg, and the methanol extract obtained by leaching at room temperature for 1 day was filtered using filter paper, and the filtrate was concentrated under reduced pressure. After dissolving the methanol extract of the nutmeg in 500 ml of 80% methanol, and extracted twice with the same amount of hexane (hexane), the hexane layer and the aqueous layer was concentrated under reduced pressure. The concentrated aqueous layer was again dissolved in 500 ml of distilled water and extracted twice with the same amount of ethylacetate and butanol. The three organic solvent extracts and the aqueous solution layer obtained above were concentrated under reduced pressure, and the control activities for the seven plant diseases were investigated in the same manner as in Example 1.

As a result, as shown in Table 2 , the ethyl acetate layer showed the highest overall control activity against the seven plant diseases, this ethyl acetate layer was used for the separation and purification of the active ingredient control.

In order to separate the active ingredient from the ethyl acetate layer (11.4 g), silica gel column chromatography (5 × 60) was used as a developing solvent with a mixture of chloroform: methanol (1% → 100% methanol concentration gradient). Cm, Kaigel gel 60, 70-230 mesh, 500 g: Merck). The eluate obtained therefrom was separated and analyzed by thin layer chromatography (Kaygel gel 60 F254, 0.25 mm; Merck) using a mixture of chloroform: methanol (99: 1 or 95: 5, v / v) as the developing solvent. . Divide into seven fractions of F1 to F7 according to the pattern of TLC (Rf value in a mixture of chloroform: methanol (95: 5); F1 => 0.7, F2 = 0.6-0.7, F3 = 0.4-0.6, F4 = 0.3-0.4, F5 = 0.1-0.3, F6 = 0.05-0.1, F7 = <0.05), each fraction was dissolved in methanol and diluted with a solution of 250 µg / ml to a concentration of 2,000 µg / ml, In the same manner as in Example 1, their control activities against seven plant diseases were investigated.

As a result, the F1, F2, F3, F4 and F5 fractions showed high control activity. From the F1 fraction (345 mg), substance 1 (191 mg) was isolated purely through Sephadex-LH20 column chromatography using methylene chloride: hexane: methanol (5: 5: 1, v / v / v). F2 fraction (1.3 g) was added to a silica gel column and eluted with hexane: ethyl acetate (2: 1, v / v) to separate substance 2 (441 mg) purely. From the F3 fraction (811 mg), two active fractions were obtained through a silica gel column using chloroform: methanol (99: 1, v / v), that is, the F31 fraction and the F32 fraction. From the F31 fraction (103 mg), substance 3 (29 mg) was isolated purely by Sephadex-LH20 column chromatography using 100% methanol. From the F32 fraction (190 mg), methylene chloride: hexane: methanol (5 : 4 (74 mg) was isolated purely through Sephadex-LH20 column using: 5: 1, v / v / v). From the F4 fraction (847 mg), substance 5 (176 mg) was purified purely through silica gel column chromatography using chloroform: methanol (95: 5, v / v) as the eluent. Finally, substance 6 (522 mg) was purely separated from the F5 fraction (2.2 g) through a silica gel column using chloroform: methanol (95: 5, v / v).

Example 3 Structure Determination of a Control Active Ingredient

Six control active ingredients isolated purely from the methanol extract of nutmeg in Example 2 were analyzed using a mass spectrometer (JEOL JMS-DX303; JEOL Ltd., Tokyo, Japan). Analysis by electron impact mass spectrum showed that substance 1 had a molecular ion [M] ⁺ at m / z 328, with a major segment ion at m / z 137 (base peak), 315 , 122, 105, 94, 77 and the like, and was estimated to be Ostrobayignan-6. In addition, as a result of ¹ H and ¹³ C-nuclear magnetic resonance analysis, substance 1 was obtained by Nakatani et al. (Nakatani et al., Phytochemistry 27: 3127-3129, 1998; Nakatani et al., Chemistry Express 2: 627-630, 1987). reported that erythromycin - Australasia Bay in lignans 6; were found to match (erythro -austrobailignan-6 abbreviated as follows, "eA-6").

Mass 2 showed that the molecular ion [M] ⁺ was found in m / z 326, and the major segment ions were m / z 308, 293, 204, 189, 175, 165, 152, 137 (base peak), 91 and the like. In addition, ¹ H and ¹³ C-nuclear magnetic resonance analysis showed that Purushothaman and Sarada, J. Chem. Soc., Perkin Trans. I , 587-588, 1977 and Pham et al. , Malabaricone-A (abbreviated as “MA”) reported by Tetrahedron 56: 1707-1713, 2000.

Mass 3 showed that [M] ⁺ was found at m / z 330, and major segment ions were detected at m / z 297, 192, 165, 152, 137 (base peak), 122, 94 and the like. In addition, it is abbreviated as meso- dihydroguaiaretic acid (hereinafter referred to as “mDA”) reported by Nakatani et al. (The homology) as a result of ¹ H and ¹³ C-nuclear magnetic resonance analysis. ).

Material 4 showed molecular ions [M] ⁺ at m / z 344 and major segment ions at m / z 192 (base peak), 177, 161, 145, 137 and 124. In addition, ¹ H and ¹³ C-nuclear magnetic resonance spectra are consistent with nectandrin-B (nectandrin-B = malabaricanone-A, hereinafter abbreviated as “NB”) reported by Nakatani et al. (Homologous). It was.

Mass spectrometry of substances 5 and 6 showed that substance 5 showed [M] ⁺ at m / z 342 and the major segment ions were m / z 165, 152, 137 (base peak), 120, 107, 91 and 77. Seemed. Material 6 showed [M] ⁺ at m / z 358 and major segment ions were m / z 248, 220, 165, 149, 137 (base peak) and 123. As a result of nuclear magnetic resonance analysis of the two materials, the ¹ H and ¹³ C-nuclear magnetic resonance spectra of the two materials were obtained from Purushothaman and Sarada (homologous) and Pham et al. (Homologous). The reported Malabaricon-B (hereinafter abbreviated as "MB") and Malabaricon-C (hereinafter abbreviated as "MC") are consistent with each other.

Example 4 Control Activity against Plant Diseases of Nutmeg Extract-Derived Active Ingredients

In order to investigate the in vivo control activity of eA-6, mDA, NB, MA, MB and MC isolated from the nutmeg extract in Example 3, rice blast, rice leaf blight, tomato gray mold disease A total of seven plant diseases, including tomato blight, wheat rust, wheat flour and pepper anthrax, were tested as follows.

After dissolving each of the six active ingredients in acetone, 250 μg / ml Tween 20 solution was added to adjust the final concentrations to 125, 250 and 500 μg / ml and the final acetone concentrations of all samples were adjusted to 10%. At this time, a solution containing 10% acetone and 250 μg / ml Tween 20 was used as a control. Two pots were used for each plant disease, and the active ingredient samples were spray-sprayed on the foliar and air-dried for 24 hours before inoculating each plant pathogen. The control activity against these seven plant diseases was investigated according to the method described in Example 1, and the results are shown in Table 3 below.

As shown in Table 3 , all six substances isolated showed high activity against rice blast and wheat rust, but little effect on tomato gray mold. The other four plant diseases showed different control activities depending on the substances. In rice leaf blight, only mDA and NB showed moderate activity in lignan-based compounds, NB showed high activity in lignan-based compounds, and only MC in resorcinol-based compounds. . Among barley powdery mildew disease, only eA-6 showed high control activity. In addition, MB showed the highest activity for pepper anthrax and all other substances except eA-6 showed moderate control activity.

Example 5 Comparison of Control Values According to Extraction Solvents

In order to compare the control activity according to the extraction solvent used to separate the active ingredient from the nutmeg, 10 g of dried samples of nutmeg were placed in a beaker, followed by hexane; Hexane / ethyl acetate (extract the remaining sample after extraction with hexane again with ethyl acetate); Ethyl acetate; Methylene chloride; Acetone; And 6 ml of methanol each was added 100 ml and leached at room temperature for 12 hours. Each solvent extract obtained from this was filtered and concentrated under reduced pressure. Each solvent extract sample was dissolved in acetone or methanol, with hexane, ethyl acetate, hexane / ethyl acetate and methylene chloride extracts dissolved in 3 ml of acetone and acetone and methanol extracts in 1.5 ml of methanol. After diluting each sample to 30 ml with 250 µg / ml Tween 20 solution, the concentration was 500, 1000 and 2000 µg / ml. The control activity against was investigated in the same manner as in Example 1.

As a result, as shown in Table 4 , the hexane extract showed the lowest control activity, while the hexane / ethyl acetate extract showed the highest control activity.

On the other hand, the six extracts were investigated for their effects on strawberry petals, fruits, leaves, haze and flower garden. Each extract was dissolved in Tween 20 solution (250 [mu] g / ml) at 1000, 2000 and 4000 [mu] g / ml and spray sprayed onto strawberries, and the results are shown in Table 5 below.

(+++: weak area> 30%; ++: weak area 5%-30%; +: weak area 1%-5%; ±: weak area 0.1-1%;-: weak)

As shown in Table 5 , the acetone extract and the methanol extract caused the strongest weakness, while the hexane extract caused the weakest weakness. Hexane / ethyl acetate extract, ethyl acetate extract and methylene chloride extract showed moderate weakness.

As described above, the composition for control of the present invention comprising the nutmeg extract, the lignan compound or the resorcinol-based compound purified therefrom and purified from the natural product is harmless to the human body and does not cause environmental pollution. It has high control against rice blast, rice leaf blight, tomato blight, wheat red rust, barley flour and pepper anthracnose, so it can be useful for developing eco-friendly natural product disinfectant and producing high value-added organic products.

Claims (7)

A nutmeg extract comprising erythro-austrobayignan-6, meso-dihydroguaiaretic acid, nectandrin-B, Malabaricon-A, Malabaricon-B, Malabaricon-C or one or more of these is effective A composition for control of rice blast, rice leaf blight, tomato late blight, wheat rust disease, barley flour or pepper anthrax containing as an ingredient. delete delete The method of claim 1, Wherein the nutmeg extract is prepared using a solvent selected from the group consisting of methanol, aqueous methanol solution, ethanol, aqueous ethanol solution, acetone, hexane, ethyl acetate, methylene chloride and mixtures thereof. The method of claim 1, The nutmeg extract is characterized in that it comprises a concentration of 100 μg / ㎖ to 3,000 μg / ㎖. The method of claim 1, Erythro-Ostrobayrignan-6, Meso-dihydroguaiaretic acid, Malabaricon-A, Malabaricon-B or Malabaricon-C are included at a concentration of 100 μg / ml to 1,000 μg / ml Composition. A method of controlling rice blast, rice leaf blight, tomato blight, wheat red rust, barley flour or red pepper anthracnose comprising treating the plant disease control composition of claim 1 to crops or soil.

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