KR20180015938A - Composition for controlling pest comprising torilin or its salt from Torilis japonica as effective component and method for controlling pest using the same - Google Patents

Abstract

The present invention relates to a composition for controlling harmful insects containing torilin isolated from Torilis japonica (Houttuyn) DC. or a salt thereof as an active ingredient, and a method for controlling harmful insects using the same. Isolated from Torilis japonica (Houttuyn) DC., the torilin is eco-friendly and is effective in controlling larvae or imagoes of at least one selected from diamondback moth, green peach aphid, and Mythimna separata (Walker, 1865).

Description

TECHNICAL FIELD The present invention relates to a composition for controlling insect pests comprising as an active ingredient tauryrin or a salt thereof isolated from casualties and a method for controlling pests using the same. same}

The present invention relates to a composition for controlling pests containing as an active ingredient tolylene or a salt thereof isolated from casualties, and a method for controlling pests using the composition.

Humans have been steadily developing since the Industrial Revolution, aiming for efficient production and consumption. In modern agriculture, pesticides are indispensable factors for stable mass production of high quality food crops. Since these pesticides vary in their use depending on the type of crops and target pests, hundreds of pesticides are manufactured and sold in the country as well . However, industrialization of agriculture based on chemical technology has resulted in many unexpected social problems such as destruction of natural ecosystems, increased pesticide resistance of pests and soil, water quality and environmental pollution. These decades of organic synthetic pesticides have caused many side effects such as the emergence of insect pests or the development of resistant pests, toxic manifestations to humans and other insects, and pollution of environmental systems. Therefore, scholars from all over the world insisted on reducing global warming and environmental pollution in order to preserve the survival environment of mankind, and finally the world nations reached an agreement. In international consultations, we agreed to gradually limit the production and use of organic synthetic pesticides, which have a significant impact on manpower. Countries around the world agreed on 50% of the total synthetic pesticide, organochlorine pesticides used in the past 10 years, And by 2010 we have agreed to reduce the production of organophosphorus and organic chlorine-based pesticides by 50%. However, after consultation on the production of organophosphorus and organic chlorine-based pesticide reduction, many researchers around the world have made great efforts to develop environmentally friendly pesticides that can replace organophosphorus and organochlorine pesticides. However, It has not been possible to develop a safe new insecticide. Therefore, it is difficult to keep the pesticide production reduction agreement in the world, and if insecticides are not produced safely in the near future and the amount of insecticides to be used becomes insufficient, it is expected that difficulties will arise not only in agriculture related to food production but also in various industries .

Pesticides used for the protection of crops should have good control effect against weeds and pests, but they should not be toxic or large for human or livestock and should not easily decompose in the environment and act as environmental pollutants. In order to solve these various problems, researches on low toxicity or non-toxic, non-pollution biological pesticides are actively conducted. Biological pesticides are classified into microbial pesticides, biochemical pesticides and natural pesticides. Microbial pesticides are agricultural microbial control agents using living microorganisms such as fungi, bacteria, viruses and protozoa, and biochemical pesticides are classified into active substances related to signal transmission or herbicides . On the other hand, biochemical pesticides include natural pesticides composed of natural ingredients such as animals and plants, pheromones, enzymes and minerals, and pesticides produced in plants by genes introduced into plants. Such bio-pesticides have relatively low persistence and do not affect man-made shrinkage and environment and are being developed for use as a substitute for chemical pesticides. However, such environmentally friendly pesticides are generally not easy to handle raw materials, and their pesticides are very low in comparison with chemical pesticides. Thus, environmentally friendly pesticides have difficulties in industrialization and have a limitation in commercialization. Most organic pesticides and natural resource-derived pesticides are expensive, low economic efficiency, low efficacy, low efficacy, extremely limited efficacy, and drug efficacy are not often effective. Users have not been able to get the response. However, due to the excessive use of chemical synthetic pesticides worldwide, environmental problems such as pollution of soil and water quality, disturbance of ecosystem due to soil microorganisms and natural enemy decrease are becoming big social issues. In recent years, the continuous and continuous use of pesticides has led to the problem of resistance to pesticides resistant to pest control even when a large amount of pesticides are sprayed more than the pesticides previously used. In addition, pesticides which are often toxic in agricultural products are detected in excess Even if the value as a food is lost. This has resulted in a variety of undesirable direct or indirect side effects. In case of pesticides which affect the human body among the pesticides remaining in the crops, it is a fatal hazard to the human body.

With the development of the national economy, the level of income has improved and consumers' perception of agricultural products has shifted, and consumers are looking for environmentally friendly agricultural products including safe food. In order to produce eco-friendly agricultural products, it is necessary to replace chemical pesticides and fertilizers. Biological pesticides are being proposed as the most important technology in comprehensive pest management for securing chemicals that can replace highly toxic organic chemical pesticides, safe for human and animal husbandry, solving environmental toxicity problems and producing environmentally friendly agricultural products. Biologically active pesticides are crop protection agents that control microorganisms, pests, and weeds that damage the production and preservation of crops using natural organisms and materials derived from them.

In the United States, the US Environmental Protection Agency exempts safety testing for natural ingredients in products classified as least hazardous pesticides. However, the Association of Special Consumer Products, which represents existing generic pesticide companies, said that because of this exemption, some moped companies are releasing their products indiscriminately to the market without proving the effectiveness of their natural products. The petition was submitted to the US Environmental Protection Agency. On the other hand, in OECD countries including Korea, we are actively promoting eco-friendly agriculture policy to reduce the use of highly toxic organic synthetic chemicals and chemical synthetic fertilizers in order to protect mankind from adverse effects such as environmental pollution and pesticide residues. In 2009, the market size of eco-friendly agricultural products increased by 17% from 2008 to 3,735.5 billion won. In 2010, when the low-pesticide new certification system is abolished, the growth rate is expected to slow down to 4.94 trillion won, and in 2013 it is estimated to be 5.955 trillion won. In 2015, when the pesticide certification system itself is abolished, it is expected that some pesticide farmers will be reduced to 4,921.6 billion won, assuming conversion to organic certification or organic certification, and then gradually recovered. It is estimated that it will be 7.767 billion won, 20% of the scale.

In Korean Patent Laid-Open Publication No. 2006-71380, a pesticide extract, a ginseng extract, a soybean extract, an sesame oil, and an infiltrant are formulated into a biocidal insecticide using a vegetable extract as a conventional technology for a known natural pesticide composition. Korean Patent No. 278796 discloses a natural insecticide as a biocidal pesticide using a plant extract which further contains a plant extract, an oil, a whitish root extract, a white pine tree extract, a coconut tree extract and a castor extract. Korean Patent No. 415107 discloses Ginkgo biloba leaf extract, preparation method and use thereof, and Korean Patent No. 338712 discloses a biocide pesticide formulation for antifungal and manufacturing method thereof. Korean Patent No. 285208 discloses a novel calcium-containing biocide US 6,051,233 discloses a natural soil treatment and pesticide composition, US 6,582,712 discloses pest control using a surfactant and terpene oil, US 6,548,085 The present inventors have disclosed a fungicide composition, but the fungicide composition has a problem with insecticidal power and is not actually used, or even if it is used, it is used only for some tests.

In addition, analyzing trends of pesticide development in recent years, besides organic synthetic pesticides, it is expected that the global biological product market will expand to more than 5 trillion won within the next 10 years, and the domestic pesticide market will also expand to 100 billion won It is expected that the possibilities will increase with the development of related biotechnology.

The currently used pesticides can be classified into neurotransmitter inhibitors, energy production inhibitors, growth regulators and sex pheromone attractants in terms of action mechanism, and the growth regulators mentioned above can be classified into glaucoma hormone inhibitors and chitin biosynthesis inhibitors.

Many researchers have been developing pesticide compositions that do not have serious toxicity and do not destroy ecosystems but have excellent effects such as sterilization, antibacterial or insecticidal effects. In order to realize eco-friendly agriculture, harmful to human and environment, And the like. Therefore, it is required to develop a pesticide which is harmless and environment friendly to humans, plants and animals, and which is easily handled, has a low production cost, and has high controllability. Therefore, it is considered that the most important goal of pesticide researchers is to develop pesticides which are not toxic to humans or livestock and do not act as pollutants in the environment. Various studies have been carried out to reduce the amount of pesticide used in various ways, There is no mention of a composition for controlling pests containing an effective ingredient of tolylene or a salt thereof separated from casualties of the present invention and a method for controlling pests using the same.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and an object of the present invention is to provide a composition for controlling pests containing taurine or a salt thereof as an active ingredient and a method for controlling pests using the composition, The present inventors have completed the present invention by confirming that the insecticidal effect is exhibited on the larva of the Chinese cabbage moth, the peach lycopene aphid, and the moth moth.

In order to achieve the above object, the present invention provides a composition for controlling pests comprising taurine or a salt thereof as an active ingredient.

The present invention also provides an agricultural or horticultural pesticide containing taurine or a salt thereof as an active ingredient.

The present invention also provides a method of controlling pests comprising treating the soil or the poultry to protect the poultry or the salt thereof from pests.

The present invention relates to a composition for controlling pests containing as an active ingredient tolylene or a salt thereof isolated from casualties, and a method for controlling pests using the composition. The taurine, which is an effective ingredient of the present invention, has an insecticidal effect on pests, and is particularly excellent in insecticidal effect of at least one adult or larva selected from cabbage moth, peach lycopene and aphid moth, , The safety is excellent.

1 is a mass spectrometry spectrum of the compound of formula (I) of the present invention.
2 is a hydrogen nuclear magnetic resonance spectrum of the compound of Formula 1 of the present invention.
3 is a carbon nuclear magnetic resonance spectrum of the compound of formula (I) of the present invention.
4 is a HMQC (Heteronuclear Multiple Quantum Coherence) spectrum of the compound of Formula 1 of the present invention.
FIG. 5 is a HMBC spectrum of the compound of Formula 1 of the present invention. FIG.
FIG. 6 shows the larval activity of the larva of the Chinese cabbage larvae according to the treatment of the compound of Chemical Formula 1 of the present invention.

The present invention relates to a composition for controlling insect pests containing, as an active ingredient, tolylene or a salt thereof represented by the following formula (1).

Preferably, the insect pest is at least one selected from the group consisting of cabbage moth, peach lycopene, and aster moth, but is not limited thereto. The taurine is called < RTI ID = 0.0 > Torilis & japonica extract, but the present invention is not limited thereto. The solvent of the caspase extract may be any one selected from water, C ₁ -C ₄ lower alcohols, ethyl acetate, and mixtures thereof. Chloroform, ethyl acetate, n-hexane and water. The fractions can be sequentially subjected to silica gel column chromatography, reversed phase column chromatography and high performance liquid chromatography (HPLC), and the above silica gel chromatography It is preferable to use chloroform and methanol as the eluting solvent, but it is not limited thereto and it is preferable to use methanol as an elution solvent in the reverse phase chromatography, but it is not limited thereto.

The present invention also relates to an agricultural or horticultural pesticide containing, as an active ingredient, tolylene or a salt thereof represented by the following general formula (1).

[Chemical Formula 1]

The insecticide of the present invention can be used to effectively control larva of insect pest which is resistant to the conventional insecticide. When the insecticide according to the present invention is used, the pesticide itself, or the hydrochloric acid or sulfuric acid, Or in the form of an agrochemically acceptable salt with an organic acid such as p-toluenesulfonic acid or the like.

In addition, the insecticide of the present invention can be mixed with a conventional solid carrier, a liquid carrier, a gas carrier or a handout, or absorbed into a basic substance such as a porous ceramic plate or a nonwoven fabric, and then added with a surfactant and, , It may be formulated into various forms such as, for example, oil sprays, emulsifiable concentrates, wettable powders, milk, granules, dust, aerosols, fumigants, vaporizable formulations, smoking formulations, toxic handouts, It can be formulated with water.

Each of the above formulations may contain 0.01 to 95% by weight of the active ingredient of the present invention. The types of solid carriers that can be used in the formulation include fine powders or granules of clay materials such as kaolin clay, diatomaceous earth, synthetic hydrated silicon oxide, bentonite, clay, clay and acid clay; Various talc, ceramics and other inorganic materials such as sericite, quartz, sulfur, activated carbon, calcium carbonate and hydrated silica; And chemical fertilizers such as, for example, ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, and ammonium chloride, but are not limited to, water; Alcohols such as methanol and ethanol; Ketones such as acetone and methyl ethyl ketone; Aromatic hydrocarbons such as benzene, toluene, xylene, ethylbenzene and methylnaphthalene; Aliphatic hydrocarbons such as hexane, cyclohexane, kerosene and light oil; Esters such as ethyl acetate and butyl acetate; Nitriles, such as acetonitrile and isobutyrnitrile; Ethers such as diisopropyl ether and dioxane; Acid amides such as N, N-dimethylformamide and N, N-dimethylacetamide; Halogenated hydrocarbons such as dichloromethane, trichloroethane and carbon tetrachloride; Dimethyl sulfoxide; And vegetable oils such as soybean oil and cottonseed oil, but the gas carrier or propellant may include, but is not limited to, freon gas, butane gas, liquefied petroleum gas, dimethyl ether, and carbon dioxide.

Base materials for use in the toxic handouts include handles materials such as grain powders, vegetable oils, sugars and crystalline cellulose; Antioxidants such as dibutylhydroxytoluene and nordihydroguaiaretic acid; Preservatives such as dehydroacetic acid; Non-foraging materials such as pepper powder; And an attractiveness flavor, such as cheese flavor and onion flavor. The surfactant may include alkyl sulfates, alkyl sulfonates, alkylaryl sulfonates, alkylaryl ethers and polyoxyethylene derivatives thereof, polyethylene glycol ethers, polyhydric alcohol esters and sugar alcohol derivatives, such as adhesives or dispersants The adjuvants include casein, gelatin; Polysaccharides such as starch, gum arabic, cellulose derivatives and alginic acid; Lignin derivatives, bentonites, sugars and synthetic water-soluble polymers such as polyvinyl alcohol, polyvinylpyrrolidone and polyacrylic acid, the stabilizers being isopropyl acid phosphate, 2,6-di-tert- A mixture of 4-methylphenol, 2-tert-butyl-4-methoxyphenol and 3-tert-butyl-4-methoxyphenol, vegetable oils, mineral oils, surfactants, fatty acids and esters thereof But is not limited to.

When the present compound is used as an agricultural insecticide, the application amount thereof is usually 0.1 to 100 g per 10 acres, but is not limited thereto. In the case of emulsifiable concentrates, wettable powders, animal and other analogous formulations used after dilution with water, the application concentration is usually in the range of 1 to 100,000 ppm. Application of granules, dust or other similar formulations is carried out as an undiluted formulation. When the compounds of the present invention are used as insecticides for epidemic prevention, they may be diluted with water to a concentration of 0.1 to 500 ppm for emulsifiable concentrates, wettable powders, animal or other similar formulations, , Aerosols, fumigants, toxic handouts, antidicking sheets or other similar formulations. The application amount and concentration may vary depending on the form of the formulation, application time, place and method, type of pest, degree of damage, and other factors, so that the increase or decrease is not limited to the above range.

The active ingredients of the present invention may be used in combination with other insecticides, nematocides, fungicides, fungicides, fungicides, herbicides, plant growth regulators, synergists, fertilizers, soil conditioners and / It can be used separately but simultaneously.

The present invention also relates to a method for controlling pests comprising the step of treating the poultry with a crop or soil to protect the poultry of the following formula (1). Preferably, the insect pest is at least one selected from the group consisting of cabbage moth, peach lycopene, and aster moth, but is not limited thereto.

The crops for protection from the pests include, but are not limited to, cabbage, corn or rice.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Example  One. Live larva  Separation and purification of active substances

Taurylin, a compound of formula 1, was isolated and purified from casualty. The casualties were purchased from Daejeon Pharmacopoeia market, cleaned with water, dried in the shade and pulverized. To 1000 g of caspase powder, 95% (v / v) ethanol was added at a weight ratio of 3 times, and the mixture was extracted at room temperature for 7 days and then filtered. The filtrate was concentrated under reduced pressure to obtain 201 g of crude extract.

In order to separate and purify the active substance from the crude extract, each fraction was separated with chloroform, ethyl acetate, n-hexane and water to determine the larval activity of the fraction. Among the fractions, the ethyl acetate fraction showed the best larval larval activity. Thus, the ethyl acetate layer was concentrated under reduced pressure to obtain 173.1 g. Then, in order to separate and purify the active substance exhibiting the larval larva activity contained in the ethyl acetate fraction, 20 g of the ethyl acetate soluble fraction was purified by silica gel column chromatography (silica gel column: 230-400 mesh, Art. 9385, Merck) Respectively. At this time, a concentration gradient system consisting of chloroform: methanol (100: 1, 90: 1, 70: 1, 50: 1, 30: 1, 15: 1, 5: 1, 1: And divided into 8 fractions. The isolated larval larval activity was measured, and the fraction having the highest larval activity was concentrated under reduced pressure to obtain 1.37 g of a fraction. In order to separate the larvae active material from the silica gel column chromatography fractions, fractions were separated by reversed phase column chromatography using 60%, 70%, 80%, and 90% methanol as elution solvents. High-performance liquid chromatography was used for the separation of the fractions having high larval activity. YMC Jsphere ODS H-80 (250 × 20 mm) was used as the column and the elution solvent was 80% (v / v) methanol was flowed at 6 ml / min, and the material was separated to obtain a pure compound. The detection of the substance was carried out at UV 210 nm, the active larval larva was a peak eluting at 32 minutes, and the peak was dried under reduced pressure to obtain 229 mg of a single active substance. Molecular weight of the compound (1), which was completely purified from the dead animal and purified purely from the dead, was measured as a colorless oil. As a result, [M + Na] ⁺ was found to be m / z 399.6 and the molecular weight (MW) 1). In the high-resolution ESI-MS, the molecular formula was estimated to be C ₂₅ H ₃₂ O ₅ . As a result of measuring the ultraviolet absorbance of the compound of Formula 1, the maximum absorbed value was found at 210 nm. Referring to the above physicochemical properties, the active substance purely isolated was dissolved in CDCl ₃ to obtain a hydrogen nuclear magnetic resonance spectrum at 300 MHz (FIG. 2), dissolved in CDCl ₃ and analyzed by carbon nuclear magnetic resonance spectroscopy (FIG. 3) at 75 MHz, HMQC (Fig. 4) and HMBC (Heteronuclear Multiple Bond Correlation) spectrum (Fig. 5), and the structure of the active material was identified. As a result, the compound of Formula 1 was in agreement with Torilin (Nakazaki M. et al 1966, Tetrahedron Lett., 37, 4499-4504).

[Chemical Formula 1]

Example  2. Cabbage moth ( Plutella xylostella  L.) For larvae Taurine  Identification of insecticidal activity

The test insects used in the present invention were Plutella xylostella L.) larvae were purchased from the Korea Research Institute of Bioscience and Biotechnology Research Center, Eun - dong, Yuseong - gu, Daejeon, Korea in June 2016.

Taurine, a compound having the larva activity of the present invention, was weighed accurately and dissolved in acetone in an appropriate amount, and then mixed with 9 times of 100 ppm of Triton X-100 aqueous solution.

Cabbage which did not use insecticide was selected during the growth of cabbage, and the cabbage leaves of the uniformly developed state were cut out into a circular shape using a 3.0 cm diameter leaf disc borer and immersed in the prepared tolylene solution for 30 seconds , And pulled out the organic solvent in the hood. The leaf treated with tauryl was placed on a Petri plate (55 × 20 mm) impregnated with filter paper impregnated with distilled water, and the larvae of the second-instar larvae of the Chinese cabbage moth previously raised were moved to a Patri plate with a soft brush so that the larvae were not damaged, The test was repeated 3 times.

The cabbage larvae treated with taurine (compound of formula (I)) were bred in a thermostatic chamber (25 ± 1 ° C., relative humidity 40 to 45%, light intensity cycle 16L: 8D), 24, 48, 72 and 96 hours The larval insecticidal rate was examined.

The control layer was tested in the same manner as in the treatment with 10% (v / v) acetone solution of 100 ppm of Triton X-100 aqueous solution 9 times without adding any insecticidal substance and treating with taurine.

The taurine activity of the larvae was investigated three times and the half-life mortality (LC ₅₀ ) was calculated by Finney's (1982) probit method. When 0-100 μg of the tauryl used in the present invention was treated to larvae of the cabbage moth, the survival rate of the larvae was measured at 24 hour intervals. In comparison with the control, The insecticidal effect was shown in larvae (Table 1 and Fig. 6).

drugs density
(占 퐂 / ml) repeat Count
(n) Insecticide number Day 4 Insectivity (%) ± SD 1 day 2 days 3 days 4 days


(1)
compound 100 One 9 3 7 9 9 100.0 0.0 2 10 4 5 8 10 3 10 5 9 9 10 10 One 10 0 6 6 8 72.4 ± 13.2 2 7 0 4 4 4 3 10 One One 6 8 One One 10 One 0 0 3 44.8 ± 13.1 2 10 0 0 2 5 3 11 0 One One 6
Control 0 One 10 0 0 0 0 0.0 ± 0.0 2 11 0 0 0 0 3 10 0 0 0 0

Example  3. Peach aphid ( Myzus persicae ) For adults Taurine  Identification of insecticidal activity

Green peach aphid was raised in an acrylic cage (30 × 30 × 30 cm) while supplying cabbage tobacco seedlings indoors. Indoor rearing conditions were 25 ~ 28 ℃, light intensity was 16L: 8D and relative humidity (RH) was 50 ~ 60%. The experiment was carried out under the same conditions as the rearing condition.

The taurine (compound of formula (I) of the present invention) was accurately weighed and diluted to various concentrations to prepare a chemical solution. The cabbage leaf slice (diameter 5 cm) was immersed in the chemical solution for 30 seconds, shade was done, and the Chinese cabbage leaf was put on a single use Petri dish (5.5 × 2 cm) with cotton and filter paper (Φ 5.5 cm) laid out. The mortality rate of the peach aphid larvae was 10, and the insecticidal rate was measured 1, 2, and 3 days after the treatments. When 0 to 100 μg of the toilirin according to the present invention was treated with peach aphid and the degree of insecticidal activity was measured at 24 hour intervals, continuous insecticidal activity was observed in comparison with the control. (Table 2).

Peach aphid ( Myzus Persicae ) Insecticidal activity of taurine on adult drugs density
(占 퐂 / ml) repeat Count
(n) Insecticide rate Day 3 Insecticide rate (%) ± SD Day 1 Day 2 Day 3


The compound of formula (1)
100 One 9 0 4 8
96.3 ± 6.4 2 10 0 6 10 3 13 0 7 13
10 One 10 0 5 10
93.9 ± 10.5 2 11 0 7 9 3 10 0 4 10
One One 11 0 4 10
78.8 ± 13.9 2 11 0 4 7 3 11 0 6 9
Control One 13 0 0 One
2.6 ± 4.4 2 10 0 0 0 3 10 0 0 0

Example  4. Moth moth ( Pseudaletia 분리자 ) For larvae Taurine  Identification of insecticidal activity

As a common name, Rice armyworm, Anchin moth was raised in an acrylic cage (30 × 30 × 30cm) while supplying silkworm artifacts indoors. Indoor rearing condition was 25 ~ 28 ℃, dark cycle time was 16 hours, 8 hour cancer cycle, and relative humidity was 50 ~ 60%. The insecticidal activity test was carried out under the same conditions as the rearing condition.

The taurine of the present invention was precisely weighed, diluted with each concentration of the test insecticide, dipped in a chemical solution for 30 seconds, and shaken to a single petri dish (5.5 x 2 cm) Φ5.5cm) and laid the cabbage leaves to shade.

Ten larvae of aster moth larvae were weighed, and the rate of insecticidal activity was examined after 1, 2, 3, and 4 days of treatment with tauririn. When 0 to 100 μg of the toilirin according to the present invention was treated to a moth moth larva and the degree of insecticidal activity was checked at 24 hour intervals, a continuous insecticidal phenomenon was observed in comparison with the control, and the insecticidal effect on the moth larva larvae in a concentration- (Table 3).

Pseudaletia separata ) Identification of tauryrin insecticidal activity against larvae drugs density
(占 퐂 / ml) repeat Count
(n) Insecticide number Day 4 Insectivity (%) ± SD 1 day 2 days 3 days 4 days


(1)
compound 100 One 10 0 0 0 5
62.6 ± 14.1
2 10 0 0 0 6 3 9 0 One One 7 10 One 9 0 2 2 4
47.0 ± 18.5
2 9 0 One 5 6 3 10 0 0 One 3 One One 10 0 0 0 0
7.0 ± 6.1
2 10 0 0 0 One 3 9 0 0 One 14
Control 0 One 10 0 0 0 0
0.0 ± 0.0
2 11 0 0 0 0 3 10 0 0 0 0

Claims (8)

1. A composition for controlling insect pests comprising, as an active ingredient, tolylene or a salt thereof represented by the following formula (1).
[Chemical Formula 1]

The composition according to claim 1, wherein the insect pest is at least one selected from the group consisting of a cabbage moth, a peach aphid, and a moth moth. The composition for controlling pests according to claim 1, wherein the toilirine is separated from the caspase extract. The insecticidal composition according to claim 3, wherein the solvent of the caspian extract is any one selected from the group consisting of water, C ₁ -C ₄ lower alcohols, ethyl acetate, and mixtures thereof. An agricultural or horticultural insecticide containing, as an active ingredient, tolylene or a salt thereof represented by the following formula (1).
[Chemical Formula 1]

A method for the control of pests comprising the step of treating the pests with a crop or soil to protect the pests from pests.
[Chemical Formula 1]

[Claim 7] The method according to claim 6, wherein the insect pest is at least one selected from the group consisting of cabbage moth, peach aphid and aphan moth. The method according to claim 6, wherein the crop for protection from the insect is Chinese cabbage, corn or rice.

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