CN107087639B - Chitin-containing virus insecticidal suspending agent and preparation method thereof - Google Patents

Chitin-containing virus insecticidal suspending agent and preparation method thereof Download PDF

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CN107087639B
CN107087639B CN201710331350.XA CN201710331350A CN107087639B CN 107087639 B CN107087639 B CN 107087639B CN 201710331350 A CN201710331350 A CN 201710331350A CN 107087639 B CN107087639 B CN 107087639B
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virus
chitin
suspending agent
insecticidal suspending
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CN107087639A (en
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梁振普
张小霞
刘雅静
张俊庆
李鹏娟
冯文霞
梅梦杰
高玉千
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Henan Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products

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Abstract

The invention discloses a virus insecticidal suspending agent containing chitin, which comprises virus raw powder, chitin and the like, wherein the concentration of the chitin in the virus insecticidal suspending agent is 30-120 mg/L; the concentration of the virus raw powder in the virus insecticidal suspending agent is 1-300 hundred million PIB/ml; the content of the stabilizer in the virus insecticidal suspending agent is 2-5 wt%; the content of the surfactant in the virus insecticidal suspending agent is 5-10 wt%; the content of the synergist in the virus insecticidal suspending agent is 0.1-0.5 wt%; the content of the light protective agent in the virus insecticidal suspending agent is 0.01-0.1 wt%; the content of the dispersant in the virus insecticidal suspending agent is 1-2 wt%; the balance being sterile water. Chitin can induce plants to generate chitinase, destroy the peritrophic membrane of insects, and promote the infection of insect viruses, thereby providing the insecticidal speed. Chitin has certain light protection to insect viruses, so that the viruses are more stable in the field. Therefore, the compounding of the chitin and the virus insecticidal suspending agent obviously improves the insecticidal capacity of the insecticide.

Description

Chitin-containing virus insecticidal suspending agent and preparation method thereof
Technical Field
The invention belongs to the technical field of biological insecticides, and particularly relates to a chitin-containing virus insecticidal suspending agent and a preparation method thereof.
Background
China is a big agricultural country, has various crop varieties and is a country with multiple and frequent biological disasters such as plant diseases and insect pests, and the plant diseases and insect pests have various varieties, high degrees, high frequency and wide regions, thereby causing great threat to the growth of crops in China. At present, agricultural and forestry pests are mainly prevented by chemical pesticides in China, the pests have drug resistance due to long-term and single use of the chemical pesticides, and in order to achieve the effect of pest control, the toxicity and the use amount of the chemical pesticides have to be improved, so that not only is great damage to food safety and ecological environment caused, but also a large number of natural enemies of the pests are killed, and the pests are rampant. Despite the above-mentioned negative effects, no more ideal alternative agents have been developed, and the continued use of chemical pesticides cannot be effectively prevented. Therefore, it is urgently required to develop an insecticide which is highly effective, free from contamination and free from side effects.
The main host of baculovirus is agriculture and forestry pests, and when the virus enters the insect body, the host insect can be infected by baculovirus and die. Polyhedrin is dissolved in the alkaline environment of insect midgut to release virus particles, quickly invade midgut cells and amplify in the order of magnitude in the cell nucleus. The amplified virus particles infect pests systemically along with the hemolymph circulation, and a large amount of virus particles are generated after all tissues of the pests are infected by the virus, and at the moment, as long as the outside lightly touches the pests, the virus particles can be instantly changed into liquid containing countless polyhedrosis viruses, so that the pests die. The liquid can be spread on the upper generation and the lower generation of pests, and has good lasting effect. Meanwhile, insect viruses have the advantages of no environmental pollution, no harm to natural enemies, difficult pest resistance generation and the like in production and use, so that the control of pests in agriculture and forestry by using baculovirus is an important development direction.
Chitin, also called chitin amine, chitosan and chitin, is the only homogeneous polysaccharide containing amino groups in nature and is named [ (1,4) -2-acetamido)2-deoxy- β -D-glucose]The molecular formula is (C)8H13NO5)nThe natural high molecular polymer is only positively charged in nature at present, belongs to straight-chain aminopolysaccharide and is also called glucosamine. Chitin is widely present in shells of lower plant fungi and marine invertebrates, the annual biosynthesis amount can reach 2000 hundred million tons, the yield in nature is only second to plant fibers, and the chitin is an inexhaustible resource for human beings. But this material has limited its widespread use because of its insoluble nature. Chitosan, a natural cationic polysaccharide, can be obtained after deacetylation of chitin, and the molecular formula is (C)6H11NO4)nThe molecular weight is about 10 ten thousand, and the polysaccharide is a polymer of glucose molecules, and the structure of the polysaccharide is similar to that of cellulose. Chitin and its derivatives have the characteristics of solubility, film-forming property, degradability, nontoxicity, adsorptivity, flocculation property, induced disease resistance, moisture retention, biocompatibility and the like, and can be widely applied to the industries of agriculture, industry, medicine, environmental protection and the like. In agricultural production, chitin has an insecticidal effect. In addition, it is widely recognized as a bio-regulator, promoting plant growth, increasing yield, improving quality, inducing broad-spectrum disease resistance of plants, and thus is known as "biomaterial of the 21 st century".
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a chitin-containing virus insecticidal suspending agent and a preparation method thereof.
The object of the invention is achieved in the following way:
a virus insecticide suspending agent containing chitin comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the chitin in the virus insecticidal suspending agent is 30-120 mg/L;
the concentration of the virus raw powder in the virus insecticidal suspending agent is 1-300 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 2-5 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5-10 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.1-0.5 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.01-0.1 wt%;
the content of the dispersant in the virus insecticidal suspending agent is 1-2 wt%;
the balance being sterile water.
The virus original powder is one of cabbage looper nuclear polyhedrosis virus, fall weber nuclear polyhedrosis virus, cotton bollworm nuclear polyhedrosis virus, diamond back moth granulosis virus and cabbage butterfly granulosis virus.
The stabilizer is glycerol.
The surfactant is at least one of sodium lignosulfonate, calcium lignosulfonate, sodium dodecyl benzene sulfonate and sodium dodecyl sulfate.
The synergist is an insect growth regulator.
The insect growth regulator is at least one of chlorbenzuron, flufenoxuron, hexaflumuron, chlorfluazuron, diflubenzuron, antifeedant urea and chlorfluazuron.
The photo-protecting agent is an aromatic amino acid.
The aromatic amino acid is at least one of tryptophan, tyrosine and phenylalanine.
The dispersing agent is at least one of sodium polyacrylate, methylene dinaphthalene sulfonate, alkyl amino taurate, polyvinyl alcohol and sodium tripolyphosphate.
The preparation method of the chitin-containing virus insecticidal suspending agent comprises the following steps:
(1) firstly, mixing virus raw powder with a synergist, sequentially adding a stabilizer, a surfactant, a light protective agent, a dispersant and sterilized water, and uniformly mixing to obtain a virus insecticidal suspending agent;
(2) and (2) adding chitin into the virus insecticidal suspending agent obtained in the step (1), and uniformly mixing to obtain the virus insecticidal suspending agent containing chitin.
Compared with the prior art, the virus insecticidal suspending agent provided by the invention has the following main advantages:
1. improves the insecticidal effect of the virus insecticide, is environment-friendly and has low cost
Chitin has insecticidal effect, and can be compounded with baculovirus insecticide to improve insecticidal effect of the insecticide. Researches find that chitin can be used as an exciton to induce the expression of a protein gene related to the disease course and induce plants to generate chitinase, and the chitinase can be used as an insecticide to directly control pests. Chitin is a major constituent of insect exoskeletons and peritrophic membranes, and typically accounts for 25-40% of the dry weight of insect body walls. The insect's peritrophic membrane and exoskeleton are important physiological barriers for insects to defend against pathogens, and chitin is an attractive potential target for insecticidal action in insects. Chitin can induce plants to generate chitinase, destroy the peritrophic membrane of insects, and promote the infection of insect viruses, thereby providing the insecticidal speed. In addition, chitin has certain light protection to insect viruses, so that the viruses are more stable in the field. Therefore, the compounding of the chitin and the virus insecticidal suspending agent obviously improves the insecticidal capacity of the insecticide. Chitin is a natural straight-chain amide polysaccharide, widely exists in the hard shell of crustaceans and the cell wall of fungi, and is one of the most abundant natural organic matters on the earth.
2. Can improve disease resistance of plant
Chitosan can inhibit the growth of various bacteria and has antifungal activity. The mechanism of the antibacterial is as follows: firstly, anions formed by amino groups of chitin and anions such as phospholipid and the like forming microbial cell walls attract each other to stimulate the freedom degree of microbes and cause fertility disorders; secondly, chitin is further degraded to enter the inside of microbial cells, so that the transcription of genetic factors DNA and RNA is blocked, and the birth inhibition is caused. The chitin can prevent the growth and development of plant pathogenic bacteria cells, induce host plants to protect pathogenic bacteria and improve the disease resistance of the plants.
3. Can improve soil property and culture soil fertility
Chitin contains a large amount of N, C elements, can be decomposed into plant nutrients by microorganisms in soil, can form a polymer film with good permeability, and improves the permeability, water retention, erosion resistance and tiltability of soil, thereby changing soil microorganism flora and inhibiting the growth and reproduction of soil pathogenic bacteria. Meanwhile, the chitin can also effectively improve the granular structure of soil and reduce the adverse effect of continuous cropping on the growth and development of crops, and is an ideal soil conditioner.
4. Increase the yield and improve the quality
Chitin can activate and enhance physiological and biochemical mechanisms of plants, promote root system cell meristem, promote root hair to occur in large quantities, make root systems more developed, and make absorption and antibiotic stronger, and chitin can induce root to secrete antibacterial substances, and prevent and treat diseases such as root rot. Researches show that when the chitin is sprayed on the leaf surfaces of plants, the photosynthesis can be improved, the accumulation of organic matters is promoted, and the chitin is particularly remarkably expressed on fruit and vegetable plants.
Detailed Description
The virus raw powder is prepared in a laboratory or purchased commercially;
chitin is prepared in the laboratory or purchased commercially.
Example 1:
a virus insecticide suspending agent containing chitin comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the chitin in the virus insecticidal suspending agent is 30-120 mg/L;
the concentration of the virus raw powder in the virus insecticidal suspending agent is 1-300 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 2-5 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5-10 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.1-0.5 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.01-0.1 wt%;
the content of the dispersant in the virus insecticidal suspending agent is 1-2 wt%;
the balance being sterile water.
The virus original powder is one of cabbage looper nuclear polyhedrosis virus, fall weber nuclear polyhedrosis virus, cotton bollworm nuclear polyhedrosis virus, diamond back moth granulosis virus and cabbage butterfly granulosis virus.
The stabilizer is glycerol.
The surfactant is at least one of sodium lignosulfonate, calcium lignosulfonate, sodium dodecyl benzene sulfonate and sodium dodecyl sulfate.
The synergist is an insect growth regulator.
The insect growth regulator is at least one of chlorbenzuron, flufenoxuron, hexaflumuron, chlorfluazuron, diflubenzuron, antifeedant urea and chlorfluazuron.
The photo-protecting agent is an aromatic amino acid.
The aromatic amino acid is at least one of tryptophan, tyrosine and phenylalanine.
The dispersing agent is at least one of sodium polyacrylate, methylene dinaphthalene sulfonate, alkyl amino taurate, polyvinyl alcohol and sodium tripolyphosphate.
The preparation method of the chitin-containing virus insecticidal suspending agent comprises the following steps:
(1) firstly, mixing virus raw powder with a synergist, sequentially adding a stabilizer, a surfactant, a light protective agent, a dispersant and sterilized water, and uniformly mixing to obtain a virus insecticidal suspending agent;
(2) and (2) adding chitin into the virus insecticidal suspending agent obtained in the step (1), and uniformly mixing to obtain the virus insecticidal suspending agent containing chitin.
Example 2:
a virus insecticide suspending agent containing chitin comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the chitin in the virus insecticidal suspending agent is 30 mg/L;
the concentration of the virus raw powder in the virus insecticidal suspending agent is 1 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 2 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.1 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.01 wt%;
the content of the dispersing agent in the virus insecticidal suspending agent is 1 wt%;
the balance being sterile water.
The virus original powder is cabbage looper nuclear polyhedrosis virus;
the stabilizer is glycerol;
the surfactant is sodium lignosulfonate;
the synergist is chlorbenzuron;
the photo-protecting agent is tryptophan;
the dispersant is sodium polyacrylate;
the rest is the same as example 1.
The compositions contained by the components and the contents of the compositions of examples 3 to 30 are shown in tables 1 to 7.
TABLE 1 compositions contained in the Components and amounts of the compositions in examples 3-6
Figure BDA0001292620760000051
Figure BDA0001292620760000061
TABLE 2 compositions contained in the components and contents of the compositions in examples 7 to 10
Figure BDA0001292620760000062
Figure BDA0001292620760000071
Table 3 compositions contained in each component and contents of each composition in examples 11 to 14
Figure BDA0001292620760000072
Figure BDA0001292620760000081
Table 4 compositions contained in each component and contents of each composition in examples 15 to 18
Figure BDA0001292620760000091
Figure BDA0001292620760000101
TABLE 5 compositions contained and amounts of each composition in examples 19-22
Figure BDA0001292620760000102
Figure BDA0001292620760000111
TABLE 6 compositions contained in the respective components and contents of the respective compositions in examples 23 to 26
Figure BDA0001292620760000112
Figure BDA0001292620760000121
TABLE 7 compositions contained in the respective components and contents of the respective compositions in examples 27 to 30
Figure BDA0001292620760000131
Figure BDA0001292620760000141
Example 31:
a virus insecticide suspending agent containing chitin comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the chitin in the virus insecticidal suspending agent is 30 mg/L;
the concentration of the virus raw powder in the virus insecticidal suspending agent is 30 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 3 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.2 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.1 wt%;
the content of the dispersing agent in the virus insecticidal suspending agent is 2 wt%;
the balance being sterile water.
The virus original powder is cabbage looper nuclear polyhedrosis virus;
the stabilizer is glycerol;
the surfactant is calcium lignosulfonate;
the synergist is chlorfluazuron;
the photo-protecting agent is tyrosine;
the dispersant is polyvinyl alcohol;
the components are added in sequence during preparation, the chitin is added after being stirred uniformly to enable the final concentration to be 30mg/L, and the cabbage looper nuclear polyhedrosis virus insecticidal suspending agent containing the chitin is obtained after being stirred uniformly and subpackaged. Pouring the prepared cabbage looper nuclear polyhedrosis virus insecticidal suspending agent containing chitin into a handheld sprayer, beating rape into round pieces by using a puncher, adding 200 cotton bollworm larvae onto the rape leaves, spraying, naturally drying, and adding into a 24-hole insect-culturing box. Meanwhile, a cabbage looper nuclear polyhedrosis virus insecticidal suspending agent preparation without chitin is used as a control (except that the control does not contain chitin, other formula components are the same as the above). The number of deaths was checked after 1d, 2d, 3 d. The death judgment standard is as follows: the insect was touched with a cotton swab and died.
TABLE 8 insecticidal Activity of Heliothis brassicae nuclear polyhedrosis virus insecticidal suspension containing chitin against Cotton bollworm
Figure BDA0001292620760000151
Table 8 shows the insecticidal activity of the cabbage looper nuclear polyhedrosis virus insecticidal suspension containing chitin against cotton bollworms, and it can be seen from Table 1 that spraying with leaves with cabbage looper nuclear polyhedrosis virus insecticidal suspension containing chitin at a concentration of 30mg/L resulted in a mortality of 3% for 24h, 9% for 48h, 29.5% for 72h and 54% for 96 h. The mortality rate of the control group was 0, 4%, 19%, 33.5%, respectively. The insecticidal activity of the cabbage looper nuclear polyhedrosis virus insecticidal suspending agent containing chitin on cotton bollworms is improved by about 20 percent compared with that of a control group.
Example 32:
a virus insecticide suspending agent containing chitin comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the chitin in the virus insecticidal suspending agent is 30 mg/L;
the concentration of the virus raw powder in the virus insecticidal suspending agent is 200 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 3 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.2 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.1 wt%;
the content of the dispersing agent in the virus insecticidal suspending agent is 2 wt%;
the balance being sterile water.
The virus raw powder is diamondback moth granules;
the stabilizer is glycerol;
the surfactant is calcium lignosulfonate;
the synergist is chlorfluazuron;
the photo-protecting agent is tyrosine;
the dispersant is polyvinyl alcohol;
the components are added in sequence during preparation, the chitin is added after being stirred uniformly to ensure that the final concentration is 30mg/L, and the diamondback moth particle virus insecticidal suspending agent containing the chitin is obtained after being stirred uniformly and subpackaged. Pouring the prepared diamondback moth granulosis virus insecticidal suspending agent containing chitin into a handheld sprayer, transferring 200 heads of diamondback moth larvae onto fresh rape leaves, treating after stabilization of the larvae for half an hour, and spraying by using the handheld sprayer until the leaves are fully wet but do not drip. After natural drying, the culture dish is moved into a culture dish, and a preservative film is added and a cover is covered. Meanwhile, a diamondback moth particle virus insecticidal suspending agent preparation without chitin is used as a control (except that the chitin is not contained, other formula components are the same as the above). The number of deaths was checked after 1d, 2d, 3 d. The death judgment standard is as follows: the insect was touched with a cotton swab and died.
TABLE 9 insecticidal Activity of chitin containing Plutella xylostella granulosis Virus insecticidal suspension on Plutella xylostella
Figure BDA0001292620760000161
As can be seen from Table 9, when leaf spraying was carried out using the diamond back moth granulosis virus insecticidal suspension having a chitin concentration of 30mg/L, the mortality rate was 4% at 24 hours, 11% at 48 hours, 31% at 72 hours and 56% at 96 hours. The mortality rate of the control group was 0, 5%, 20.5%, 36.5%, respectively. The insecticidal activity of the chitin-containing diamond back moth granulosis virus insecticidal suspending agent on diamond back moths is improved by about 20 percent compared with that of a control group.
Example 33:
a virus insecticide suspending agent containing chitin comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the virus raw powder in the virus insecticidal suspending agent is 200 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 3 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.2 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.1 wt%;
the content of the dispersing agent in the virus insecticidal suspending agent is 2 wt%;
the balance being sterile water.
The virus raw powder is diamondback moth granules;
the stabilizer is glycerol;
the surfactant is calcium lignosulfonate;
the synergist is chlorfluazuron;
the photo-protecting agent is tyrosine;
the dispersant is polyvinyl alcohol;
the components are added in sequence during preparation, the chitin is added after being stirred uniformly to lead the final concentration to be respectively 30mg/L, 60mg/L and 120mg/L, and the cabbage looper nuclear polyhedrosis virus insecticidal suspending agent containing the chitin is obtained after being stirred uniformly and subpackaged. Adding 1ml of the virus insecticide with each concentration into 12ml of potato sucrose agar (PDA) culture medium which is heated to melt and is about 60 ℃, uniformly mixing, pouring into a culture dish to prepare virus insecticide flat plates containing chitin with different concentrations, taking equivalent sterile water as a reference, inoculating a pathogenic bacterium strain sheet with the diameter of 0.8cm at the center of the flat plate after solidification, culturing at constant temperature of 28 ℃, recording the diameter of a bacterial colony day by day, and repeating the treatment for 3 times. After the blank control mycelium had fully grown, the diameter of each treated mycelium was measured and the inhibition for each treatment was calculated. The method for measuring the colony expansion diameter and calculating the inhibition rate comprises the following steps: colonies were measured 2 times by the cross method, and the average number thereof was used to represent the size of the colonies. The colony expansion diameter and the inhibition rate of the pesticide are calculated according to the formula.
In the formula, I is the bacteriostasis rate, D1 is the average diameter of the control mycelium, and D0 is the average value of the treated mycelium.
As is clear from Table 10, the viral insecticide containing chitin had inhibitory effects on 10 pathogenic fungi, and the effect was enhanced with increasing concentration.
TABLE 10 inhibition rate of cabbage looper nuclear polyhedrosis virus insecticidal suspension containing chitin at different concentrations against 10 pathogenic bacteria
Figure BDA0001292620760000181
Example 34:
a virus insecticide suspending agent containing chitin comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the virus raw powder in the virus insecticidal suspending agent is 200 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 3 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.2 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.1 wt%;
the content of the dispersing agent in the virus insecticidal suspending agent is 2 wt%;
the balance being sterile water.
The virus raw powder is diamondback moth granules;
the stabilizer is glycerol;
the surfactant is calcium lignosulfonate;
the synergist is chlorfluazuron;
the photo-protecting agent is tyrosine;
the dispersant is polyvinyl alcohol;
the components are added in sequence during preparation, the chitin is added after being stirred uniformly to enable the final concentration to be 30mg/L, and the cabbage looper nuclear polyhedrosis virus insecticidal suspending agent containing the chitin is obtained after being stirred uniformly and subpackaged. The prepared diamond back moth granulosis virus insecticidal suspending agent containing the chitin is poured into a hand-held sprayer, and the spraying is carried out until the plant leaves are completely wet. During the growth of the vegetables, treatment was performed once a week while using a cabbage looper nuclear polyhedrosis virus insecticidal suspension containing no chitin as a control (except for containing no chitin, the other formulation ingredients were the same as above).
TABLE 11 Effect of chitin-containing Heliothis virescens nuclear polyhedrosis virus insecticidal suspension on the quality and yield of cherry tomatoes
Figure BDA0001292620760000191
As can be seen from table 11, the chitin treatment significantly increased the yield of cherry tomatoes by 16.2% compared to the control group in the case of containing and not containing chitin. Meanwhile, the virus insecticide containing chitin can promote the increase of Vc content in tomato fruits, and is improved by 20.7 percent compared with a control group. The total acidity of tomatoes treated with the chitin-containing viral insecticide was slightly increased and the sugar-acid ratio was also reduced.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the overall concept of the present invention, and these should also be considered as the protection scope of the present invention.

Claims (9)

1. A viral insecticidal suspending agent containing chitin is characterized in that: comprises virus raw powder, chitin, a stabilizer, a surfactant, a synergist, a light protective agent, a dispersant and water, wherein:
the concentration of the chitin in the virus insecticidal suspending agent is 30-120 mg/L;
the virus raw powder is one of cabbage looper nuclear polyhedrosis virus, fall weber nuclear polyhedrosis virus, cotton bollworm nuclear polyhedrosis virus, diamond back moth granulosis virus and cabbage butterfly granulosis virus, and the concentration of the virus raw powder in the virus insecticidal suspending agent is 1-300 hundred million PIB/ml;
the content of the stabilizer in the virus insecticidal suspending agent is 2-5 wt%;
the content of the surfactant in the virus insecticidal suspending agent is 5-10 wt%;
the content of the synergist in the virus insecticidal suspending agent is 0.1-0.5 wt%;
the content of the light protective agent in the virus insecticidal suspending agent is 0.01-0.1 wt%;
the content of the dispersant in the virus insecticidal suspending agent is 1-2 wt%;
the balance being sterile water.
2. The chitin-containing pesticidal viral suspension concentrate of claim 1, wherein: the stabilizer is glycerol.
3. The chitin-containing pesticidal viral suspension concentrate of claim 1, wherein: the surfactant is at least one of sodium lignosulfonate, calcium lignosulfonate, sodium dodecyl benzene sulfonate and sodium dodecyl sulfate.
4. The chitin-containing pesticidal viral suspension concentrate of claim 1, wherein: the synergist is an insect growth regulator.
5. The chitin-containing pesticidal viral suspension concentrate of claim 4, wherein: the insect growth regulator is at least one of chlorbenzuron, flufenoxuron, hexaflumuron, chlorfluazuron, diflubenzuron and chlorfluazuron.
6. The chitin-containing pesticidal viral suspension concentrate of claim 1, wherein: the photo-protecting agent is an aromatic amino acid.
7. The chitin-containing pesticidal viral suspension concentrate of claim 6, wherein: the aromatic amino acid is at least one of tryptophan, tyrosine and phenylalanine.
8. The chitin-containing pesticidal viral suspension concentrate of claim 1, wherein: the dispersing agent is at least one of sodium polyacrylate, methylene dinaphthalene sulfonate, alkyl amino taurate, polyvinyl alcohol and sodium tripolyphosphate.
9. The process for preparing a viral insecticidal suspension containing chitin according to any one of claims 1-8, wherein: the method comprises the following steps:
(1) firstly, mixing virus raw powder with a synergist, sequentially adding a stabilizer, a surfactant, a light protective agent, a dispersant and sterilized water, and uniformly mixing to obtain a virus insecticidal suspending agent;
(2) and (2) adding chitin into the virus insecticidal suspending agent obtained in the step (1), and uniformly mixing to obtain the virus insecticidal suspending agent containing chitin.
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CN101223881A (en) * 2007-01-18 2008-07-23 青岛科技大学 Desinsection acaricides compounds mixed with abamectin or banleptm
US7609848B2 (en) * 2000-12-29 2009-10-27 Contentguard Holdings, Inc. Multi-stage watermarking process and system
CN106577764A (en) * 2016-11-11 2017-04-26 河南农业大学 Mamestra brassicae nuclear polyhedrosis virus-containing pesticidal suspending agent and preparation method thereof

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Publication number Priority date Publication date Assignee Title
US7609848B2 (en) * 2000-12-29 2009-10-27 Contentguard Holdings, Inc. Multi-stage watermarking process and system
CN101223881A (en) * 2007-01-18 2008-07-23 青岛科技大学 Desinsection acaricides compounds mixed with abamectin or banleptm
CN106577764A (en) * 2016-11-11 2017-04-26 河南农业大学 Mamestra brassicae nuclear polyhedrosis virus-containing pesticidal suspending agent and preparation method thereof

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