CN108617676A - A kind of combination sterilization composition of prevention lawn reaping hook droop - Google Patents

A kind of combination sterilization composition of prevention lawn reaping hook droop Download PDF

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Publication number
CN108617676A
CN108617676A CN201810950741.4A CN201810950741A CN108617676A CN 108617676 A CN108617676 A CN 108617676A CN 201810950741 A CN201810950741 A CN 201810950741A CN 108617676 A CN108617676 A CN 108617676A
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CN
China
Prior art keywords
agent
trichlamide
active constituent
lawn
rosickyite azoles
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CN201810950741.4A
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Chinese (zh)
Inventor
张国利
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GUIZHOU DAOYUAN BIOTECHNOLOGY CO Ltd
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GUIZHOU DAOYUAN BIOTECHNOLOGY CO Ltd
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Priority to CN201810950741.4A priority Critical patent/CN108617676A/en
Publication of CN108617676A publication Critical patent/CN108617676A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The combination sterilization composition that prevention lawn reaping hook droop disclosed by the invention is controlled, which is aqueous suspension agent, including active constituent and auxiliary agent, and wherein active constituent compounds to obtain with trichlamide using rosickyite azoles as base-material, and auxiliary agent includes:The mass ratio of wetting dispersing agent, thickener, antifreezing agent and antifoaming agent, various composition is as follows:Active constituent 5%~50%, wetting dispersing agent 2%~6%, thickener 0.1%~0.4%, antifreezing agent 3%~5%, antifoaming agent 0.4%~0.6% add water to 100%.The quality proportioning of rosickyite azoles and trichlamide is 1: 4~4: 1 in the active constituent.The combination sterilization composition of the prevention lawn reaping hook droop of the present invention, after two kinds of base-materials compound, addition with obvious effects is safe to use, has good cytotoxicity for the sickle-like bacteria for causing lawn withered, and be apparently higher than rosickyite azoles and trichlamide single dose.Suitable for town and country for preventing lawn reaping hook droop.

Description

A kind of combination sterilization composition of prevention lawn reaping hook droop
Technical field
The present invention is related with fungicide, specifically, belonging to the fungicide containing organic compound, furthermore, it is understood that belonging to Prevent the bactericidal composition of lawn reaping hook droop.
Technical background
Fusarium wilt can cause rotten withered turfgrass seedling, root-rot, neck base, tikka and leaf corruption, stolon and root-like stock rotten Rotten equal a series of complex symptom.It is infected before and after coming up, seed root rot browning color causes rotten bud and seedling withered when serious. When morbidity is lighter, seedling Huang is thin, depauperation.Strain blade is aggrieved, is initially the withered spot of water stain shape dirty-green on sick leaf, rear to become For bronzing to brown, scab is mostly downwards or withered and yellow from the upward browning of leaf sheath base from blade tip.Thin and delicate plant base portion has bronzing long Shape scab, the positions dry rot such as root, rootstock, root-like stock and stolon become brown or bronzing, and color change portion can also be from rootstock to stem Culm base develops, and it is rotten to form base.When moist, there is white between rootstock and basal part of stem leaf sheath and stalk to pale red mycelium and divide Raw spore ball.
Rosickyite azoles also known as albendazole,(English is entitledAlbendazole, molecular formula is C12H15N3O2S, molecular weight are 265.33)It is a kind of active constituent of people's insecticide, 2012, Guizhou road combined Beijing of member biology plant pesticide research University of TCM, Agricultural University Of Nanjing and beneficial one hundred pharmacy Miao Yao R&D teams successfully develop the innovative green bactericide of rosickyite azoles, The fungicide mixed morbidity evil a variety of to plant and pathogenic infection have inhibiting effect, belong to the agriculture of absorbability rosickyite azole a new generation With fungicide, there is prevention & protection, interior suction treatment, infiltration conduction.The fungicide wide sterilization spectrum has stronger effect side The complementarity of formula and bioactivity is notable with the effect of other composite reagent synergisms.For in efficient, less toxic, real wide spectrum Absorption fungicide, it may have the feature that activity is high, the lasting period is long, absorbability is strong, spraying time is flexible has brown spot preferably anti- Effect.
Trichlamide(English is entitledTrichlamide,Molecular formula is C13H16Cl5NO3, molecular weight 340.65)To aspergillus Belong to and Penicillium fungi and chactomium globosum, bread mold, reaping hook are mould etc. extremely strong inhibition, to Escherichia coli, golden yellow Portugal The various microorganisms such as grape coccus, tangerine straw bacterium have extremely strong killing effect, have special efficacy to lawn droop.
The patent for preventing lawn reaping hook droop involved in Chinese patent database has:No. 2014101743266《One plant of Kazakhstan Thatch trichoderma and its application in the reaping hook droop of prevention and control lawn》, it is to use microorganism Trichoderma harzianum (Trichoderma Harzianum) JTM55 is good to Fusarium oxysporum control effect and stablizes.It there is no and compounded with rosickyite azoles and trichlamide at present Prevent the application part of the bactericidal composition of lawn reaping hook droop.
Invention content
The present invention is intended to provide a kind of combination sterilization composition of prevention lawn reaping hook droop, it is to use rosickyite azoles and water The bactericidal composition of poplar bacterium amine compounding, to prevent lawn reaping hook droop.
Inventor with the pesticide for having favorable effect to droop to carrying out repeated screening, and to the bulk pharmaceutical chemicals of compound composition Quality proportioning carries out repetition test, is compared to test result, finds a kind of compound disinfectant, have synergistic effect, to grass The stronger poisoning ability of germ of level ground reaping hook droop.
The combination sterilization composition for the prevention lawn reaping hook droop that inventor provides is aqueous suspension agent, including active constituent With auxiliary agent, wherein active constituent compounds to obtain with trichlamide using rosickyite azoles as base-material, and auxiliary agent includes:Wetting dispersing agent, Thickener, antifreezing agent and antifoaming agent;
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Antifoaming agent 0.4%~0.6%
Water adds to 100%;
The quality proportioning of rosickyite azoles and trichlamide is 1: 4~4: 1 in the active constituent;The rosickyite azoles is mass fraction 98% active compound, trichlamide are standard items.
Preferably, the active constituent is that rosickyite azoles is obtained with trichlamide according to the compounding of quality proportioning 2: 1.
In the auxiliary agent, wetting dispersing agent is agriculture breast 600-1, and thickener is xanthan gum, and antifreezing agent is ethylene glycol, defoaming Agent is methanol.
According to experimental result, inventor determines, has to lawn reaping hook droop with the bactericidal composition that said ratio is prepared There is synergistic effect.
The Bioexperiment for preventing the combination sterilization composition of lawn reaping hook droop is as follows:
Rosickyite azoles used is produced by Guizhou Daoyuan Biotechnology Co., Ltd.;Trichlamide used is had by Shanghai Zhen Zhun biotechnologies Limit company supplies.
1. toxicity measures
1.1 Study on Fungicide Toxicity
It is measured for examination single dose to apple charcoal using using mycelial growth rate method with reference to farm-chemical indoor determination test rule The inhibiting rate of subcutaneous ulcer disease.The PDA culture medium mixing melted with 4.5ml using mycelial growth rate method, 0.5ml liquids, is poured into sterile Culture dish in be made band medicine culture medium flat plate.After culture medium solidification, 1 bacteria cake for trying bacterium is put into each culture base plane (A diameter of 0.5cm), so that bacteria cake is attached to media surface on one side with mycelia, often handle 3 repetitions, with alcohol solvent and contain 0.1% Tween 80 aqueous solution is control.After cultivating 72h at 25 DEG C, measured with crossing method for trying bacterium colony growth diameter. Inhibiting rate is calculated according to the following formula.And by probit analysis find out the toxicity regression curves of salicyloyl enamine, hymexazo single doses with EC50 values, SD and 95% confidence limit.
Mycelial growth inhibition rate(%)=(Compare colony growth diameter-processing colony growth diameter)/ control bacterium colony growth is straight Diameter × 100
The complex role of 1.2 rosickyite azoles and trichlamide is studied
Rosickyite azoles and trichlamide are compounded:Two kinds of active compounds are configured to 4: 1,3: 1,2: 1,1: 1,1: 2 by active ingredient, 1: 3,1:4 totally 7 quality proportionings.Above-mentioned formula presses Study on Fungicide Toxicity method and carries out toxicity test respectively.
Mixture synergy is evaluated, with EC50Based on value, mixture joint is evaluated by calculating mixture co-toxicity coefficient Type of action.
CTC120 is synergistic effect, 80≤CTC<120 be summation action, CTC<80 be antagonism.
2. test result
2.1 Study on Fungicide Toxicity results
The Toxicity Determination result of the 12 kinds of medicaments of table to lawn reaping hook droop
Treatment agent Virulence regression equation EC50(mg·L–1 Related coefficient SD CL0.95
Rosickyite azoles y= 0.8167x +3.4406 82.0230 0.9487 0.3748 15.1098~445.2586
Trichlamide y= 2.5108x +1.2843 33.5242 0.9489 0.1822 14.7296~76.3002
As it can be seen from table 1 EC of the rosickyite azoles to lawn reaping hook droop50For 82.0230 μ g/mL;Trichlamide is to lawn sickle The EC of knife droop50For 33.5242 μ g/mL.
2 rosickyite azoles of table compounds the The Toxicity Determination result to lawn reaping hook droop with trichlamide
Rosickyite azoles: trichlamide Virulence regression equation Related coefficient EC50(μg/mL) Co-toxicity coefficient
1∶4 y = 0.8635x + 3.9401 0.7882 18.0933 210.1353
1∶3 y = 0.93x + 3.8804 0.9892 16.0607 244.9415
1∶2 y = 0.9888x + 3.7268 0.9022 18.5732 224.8050
1∶1 y = 1.5998x + 2.7669 0.9930 24.8390 191.6157
2∶1 y = 1.0598x + 3.6182 0.9875 20.3348 272.1329
3∶1 y = 0.6928x + 3.9356 0.9484 34.1055 176.6198
4∶1 y = 1.6999x + 2.417 0.9881 32.4890 195.8092
Interpretation of result:As shown in Table 2, rosickyite azoles and all proportions of trichlamide compounding show certain synergistic effect, But different compound proportion coefficient of synergism difference.Wherein, when the compound proportion of rosickyite azoles and trichlamide is 2: 1, poison is altogether Number highest is 272.1329;When the compound proportion of rosickyite azoles and trichlamide is 1: 3, co-toxicity coefficient time is high, is 244.9415.
The combination sterilization composition of the prevention lawn reaping hook droop of the present invention, after two kinds of base-materials compound, addition with obvious effects makes With safety, there is good cytotoxicity for the sickle-like bacteria for causing lawn withered, and be apparently higher than rosickyite azoles and trichlamide single dose. Suitable for town and country for preventing lawn reaping hook droop.
Specific implementation mode
Embodiment 1
It takes the mass fraction that Guizhou Daoyuan Biotechnology Co., Ltd. produces to be 98% rosickyite azoles active compound 3.0kg, then takes Shanghai The trichlamide standard items 1.5kg of the bio tech ltd Zhen Zhun supply is re-dubbed the compound sterilizing combination of 4.5 kg preventions The base-material of object;
It is ethylene glycol 0.4 to take 0.4 kg of wetting dispersing agent agriculture breast 600-1,0.02 kg of thickener xanthan gum, antifreezing agent again Kg, 0.05 kg of antifoaming agent methanol add water until 10kg, obtains the combination sterilization composition that prevention lawn reaping hook droop is controlled 10kg。
Embodiment 2
It takes the mass fraction that Guizhou Daoyuan Biotechnology Co., Ltd. produces to be 98% rosickyite azoles active compound 1.0kg, then takes Shanghai The trichlamide standard items 3.0kg of the bio tech ltd Zhen Zhun supply is re-dubbed 4.5 kg prevention lawn reaping hook droops Combination sterilization composition base-material;
It is ethylene glycol 0.4 to take 0.3 kg of wetting dispersing agent agriculture breast 600-1,0.02 kg of thickener xanthan gum, antifreezing agent again Kg, 0.05 kg of antifoaming agent methanol add water until 10kg, obtains the combination sterilization composition that prevention lawn reaping hook droop is controlled 10kg。

Claims (3)

1. a kind of combination sterilization composition that prevention lawn reaping hook droop is controlled, it is characterised in that the combination sterilization composition is water Suspending agent, including active constituent and auxiliary agent, wherein active constituent compound to obtain with trichlamide using rosickyite azoles as base-material, help Agent includes:Wetting dispersing agent, thickener, antifreezing agent and antifoaming agent;
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Antifoaming agent 0.4%~0.6%
Water adds to 100%;
The quality proportioning of rosickyite azoles and trichlamide is 1: 4~4: 1 in the active constituent;The rosickyite azoles is mass fraction 98% active compound, trichlamide are standard items.
2. combination sterilization composition as described in claim 1, it is characterised in that the combination sterilization composition is aqueous suspension agent, packet Active constituent and auxiliary agent are included, wherein active constituent compounds to obtain with trichlamide using rosickyite azoles as base-material, and auxiliary agent includes:Profit Hygroscopic water powder, thickener, antifreezing agent and antifoaming agent;
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Antifoaming agent 0.4%~0.6%
Water adds to 100%;
The quality proportioning of rosickyite azoles and trichlamide is 2: 1 in the active constituent;The rosickyite azoles is the original of mass fraction 98% Medicine, trichlamide are standard items.
3. combination sterilization composition as described in claim 1, it is characterised in that in the auxiliary agent, wetting dispersing agent is agriculture breast 600-1, thickener are xanthan gum, and antifreezing agent is ethylene glycol, and antifoaming agent is methanol.
CN201810950741.4A 2018-08-20 2018-08-20 A kind of combination sterilization composition of prevention lawn reaping hook droop Pending CN108617676A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113287621A (en) * 2021-05-07 2021-08-24 广东省农业科学院果树研究所 Application of imidazole derivative A in preparation of pesticide bactericide for preventing and treating banana fusarium wilt

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1930971A (en) * 2005-09-15 2007-03-21 南京第一农药有限公司 Germicide composition containing trichlamide and propiconazol and its application
CN106035349A (en) * 2016-03-29 2016-10-26 日本农药株式会社 Agricultural composition for preventing and removing harmful organisms and application method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1930971A (en) * 2005-09-15 2007-03-21 南京第一农药有限公司 Germicide composition containing trichlamide and propiconazol and its application
CN100360019C (en) * 2005-09-15 2008-01-09 南京第一农药有限公司 Germicide composition containing trichlamide and propiconazol and its application
CN106035349A (en) * 2016-03-29 2016-10-26 日本农药株式会社 Agricultural composition for preventing and removing harmful organisms and application method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113287621A (en) * 2021-05-07 2021-08-24 广东省农业科学院果树研究所 Application of imidazole derivative A in preparation of pesticide bactericide for preventing and treating banana fusarium wilt

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