CN109105384A - A kind of combination sterilization composition for preventing and treating pear tree black heart - Google Patents
A kind of combination sterilization composition for preventing and treating pear tree black heart Download PDFInfo
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- CN109105384A CN109105384A CN201811232599.6A CN201811232599A CN109105384A CN 109105384 A CN109105384 A CN 109105384A CN 201811232599 A CN201811232599 A CN 201811232599A CN 109105384 A CN109105384 A CN 109105384A
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- agent
- active constituent
- flusilazole
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- pear tree
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of combination sterilization compositions for preventing and treating pear tree black heart, the combination sterilization composition is aqueous suspension agent, including active constituent and auxiliary agent, wherein active constituent is to be compounded with rosickyite azoles with Flusilazole base-material, and auxiliary agent includes: wetting dispersing agent, thickener, antifreezing agent and defoaming agent, the mass ratio of various composition is as follows: active constituent 5%~50%, wetting dispersing agent 2%~6%, thickener 0.1%~0.4%, antifreezing agent 3%~5%, defoaming agent 0.4%~0.6%, adds water to 100%.The quality proportioning of rosickyite azoles and Flusilazole is 1: 4~4: 1 in the active constituent.Composition of the invention, effect is obviously added after two kinds of base-materials compound, and use is safe, has good cytotoxicity to pear tree black heart main pathogenic bacteria, and be higher than rosickyite azoles and Flusilazole single dose.Black heart is prevented and treated suitable for town and country plantation pear tree.
Description
Technical field
The present invention is related with fungicide, specifically, belonging to the fungicide containing organic compound, furthermore, it is understood that belonging to
The bactericidal composition for preventing and treating pear tree black heart.
Technical background
Pear tree black heart is caught an illness for fruit, and light green spot of coming into being, round or ellipse, gradual change brown to black, surface also produces
The raw velvet-like mould layer of black, scab are gradually recessed, and harden, starlike cracking often occurs.Germ is mainly with mycelium in sick branch and perula
It is interior or overwintering in sick leaf with pseudothecium.Fruit is caught an illness, light green spot of coming into being, round or ellipse, gradual change brown to black,
Surface also generates the velvet-like mould layer of black, and scab is gradually recessed, and starlike cracking often occurs for hardening.With saprophytic on later period scab
Bacterium is the native powder mould of pink colour and the sickle-like bacteria of lightpink.Young fruit is caught an illness normal teratogenesis shape.Tender tip is caught an illness, and dark brown oblong is formed
Scab, recess form sometimes in height sense kind and steep swollen shape.Floral organ is caught an illness, and petal fades, sepal tip scab gray, flower
Stalk turns black, and colored and young fruit is caused to fall off when forming ring cutting.
Rosickyite azoles also known as albendazole, (English is entitledAlbendazole, molecular formula is C12H15N3O2S, molecular weight are
265.33) be a kind of people's insecticide active constituent, 2012, Guizhou road combined Beijing of member biology plant pesticide research
University of TCM, Agricultural University Of Nanjing and beneficial one hundred pharmacy Miao Yao R&D team successfully develop the innovative green bactericide of rosickyite azoles,
The fungicide mixed morbidities a variety of to plant are harmful and pathogenic infection has inhibiting effect, belong to the agriculture of absorbability rosickyite azole a new generation
With fungicide, there is prevention & protection, interior suction treatment, infiltration conduction.The fungicide wide sterilization spectrum has stronger effect side
The complementarity of formula and bioactivity is significant with the effect of other composite reagent synergisms.For in efficient, less toxic, real wide spectrum
Absorption fungicide, it may have the feature that activity is high, the lasting period is long, absorbability is strong, spraying time is flexible has brown spot preferably anti-
Effect.
Flusilazole (English nameflusilazole, molecular formula is C16H15F2N3Si, molecular weight 315.4), it can be used for absorbability
Sterilization.It can inhibit sterol demethylation.Prevention and treatment Ascomycetes are primarily useful for, Basidiomycetes and Fungi Imperfecti fungi are effective, such as
Venturia inaequalis, Powdery Mildew, the wheat nuclear cavity bacteria of Cereal, Septoria bacterium, snag shell bacterium etc., on Guignardia and sweet tea Lay
Various pathogens, leaf spot of peanut are efficient to sclerotinia sclerotiorum.Flusilazole category triazole type systemic fungicide is destroyed and is prevented
The biosynthesis of ergosterol causes cell membrane that cannot be formed, and keeps germ dead.To caused by sac fungus, basidiomycetes and Fungi Imperfecti
Disease is effective, invalid to oomycetes, has special efficacy to pear scab.
It there is no the application part for being related to preventing and treating pear tree black heart in Chinese patent database at present, more without using rosickyite azoles and fluorine
The application part of the bactericidal composition of silicon azoles compounding prevention and treatment pear tree black heart.
Summary of the invention
The present invention is intended to provide a kind of combination sterilization composition for preventing and treating pear tree black heart, it is with rosickyite azoles and Flusilazole
The bactericidal composition of compounding, to improve the yield and quality of pear fruit.
Inventor carries out repeated screening to the pesticide that pear tree black heart has favorable effect to having, and to the original of compound composition
Expect that medicine quality proportioning carries out repetition test, test result compared, a kind of compound disinfectant is found, has synergistic effect,
To the stronger poisoning ability of pear tree black heart germ.
The combination sterilization composition for the prevention and treatment pear tree black heart that inventor provides is aqueous suspension agent, including active constituent with help
Agent, wherein active constituent is compounded using rosickyite azoles with Flusilazole as base-material, auxiliary agent include: wetting dispersing agent, thickener,
Antifreezing agent and defoaming agent；
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Antifreezing agent 3%~5%
Defoaming agent 0.4%~0.6%
Water adds to 100%；
The quality proportioning of rosickyite azoles and Flusilazole is 1: 4~4: 1 in the active constituent.
Preferably, the active constituent is that rosickyite azoles compounds to obtain with Flusilazole according to quality proportioning 1: 4.
In the auxiliary agent, wetting dispersing agent is agriculture breast 600-1, and thickener is xanthan gum, and antifreezing agent is ethylene glycol, defoaming
Agent is methanol.
According to experimental result, inventor is determined, the bactericidal composition prepared with said ratio is to prevention and treatment pear tree black heart tool
There is synergistic effect.
The Bioexperiment for preventing and treating the combination sterilization composition of pear tree black heart is as follows:
The raw medicine that rosickyite azoles used is 98%, is produced by Guizhou Daoyuan Biotechnology Co., Ltd.；Flusilazole used is mass fraction
95% raw medicine, byHubei Mao Er irrigates biological medicine Co., LtdProduction.
1. toxicity measures
1.1 Study on Fungicide Toxicity
Referring to farm-chemical indoor determination test rule, using mycelial growth rate method is used, measurement is for examination single dose to light grey and pinkish
The inhibiting rate of mildew.Using mycelial growth rate method, the PDA culture medium that 0.5ml medical fluid and 4.5ml melt is mixed, and is poured into sterile
Culture dish in be made band medicine culture medium flat plate.After culture medium solidification, 1 bacteria cake for trying bacterium is put into each culture base plane
(diameter 0.5cm) makes bacteria cake be attached to media surface on one side with mycelia, and 3 repetitions of every processing with alcohol solvent and contain
0.1% Tween 80 aqueous solution is control.After cultivating 72h at 25 DEG C, with crossing method measurement for trying bacterium colony growth diameter.
Inhibiting rate is calculated according to the following formula.And rosickyite azoles, the toxicity regression curve of fludioxonil single dose and EC50 are found out by probit analysis
Value, SD and 95% confidence limit.
Mycelial growth inhibition rate (%)=(control colony growth diameter-processing colony growth diameter)/control bacterium colony growth is straight
Diameter × 100
The research of the complex role of 1.2 rosickyite azoles and Flusilazole
Rosickyite azoles and Flusilazole are compounded: two kinds of raw medicines are configured to 4: 1,3: 1,2: 1,1: 1,1: 2,1 by effective component:
3,1: 4 totally 7 quality proportionings.Above-mentioned formula presses Study on Fungicide Toxicity method respectively and carries out toxicity test.
Mixture synergy is evaluated, with EC50Based on value, mixture joint is evaluated by calculating mixture co-toxicity coefficient
Type of action.
CTC120 be synergistic effect, and 80≤CTC < 120 are summation action, and CTC < 80 are antagonism.
2. test result
2.1 Study on Fungicide Toxicity results
The Toxicity Determination result of the 12 kinds of medicaments of table to pear tree black heart
| Treatment agent | Virulence regression equation | EC50(mgL–1) | Related coefficient | SD | CL0.95 |
| Rosickyite azoles | y = 1.561x + 1.881 | 98.7906 | 0.9726 | 0.1407 | 52.3580~186.4010 |
| Flusilazole | y = 1.229x + 4.277 | 3.9751 | 0.9668 | 0.1837 | 1.7349~9.1081 |
As it can be seen from table 1 EC of the rosickyite azoles to pear tree black heart50For 98.7906 μ g/mL；Flusilazole is to pear tree black heart
EC50For 3.9751 μ g/mL.
2 rosickyite azoles of table compounds the The Toxicity Determination result to pear tree black heart with Flusilazole
As shown in Table 2, rosickyite azoles and all proportions of Flusilazole compounding show certain synergistic effect, but different compounding ratios
Example coefficient of synergism difference.Wherein, when the compound proportion of rosickyite azoles and Flusilazole is 1: 4, co-toxicity coefficient highest is
263.0264。
The combination sterilization composition of prevention and treatment pear tree black heart of the invention, after two kinds of base-materials compound, effect is obviously added, and is made
With safety, there is good cytotoxicity for the pathogenic bacteria of pear tree black heart, and is higher than rosickyite azoles and Flusilazole single dose.It is suitable for
It plants pear tree and prevents and treats black heart in rural area.
Specific embodiment
The compound proportion of 1 rosickyite azoles of embodiment and Flusilazole is 1: 1
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd. to produce is 98% rosickyite azoles raw medicine 1.0kg, then takesHubei Cat Er Wo biological medicine Co., LtdThe flusilazole 1.0kg of the mass fraction 95% of production is re-dubbed 2.0 kg prevention and treatment pears
Set the base-material of the combination sterilization composition of black heart；
0.4 kg of wetting dispersing agent agriculture breast 600-1,0.02 kg of thickener xanthan gum, 0.4 kg of antifreeze glycol are taken again,
0.05 kg of defoaming agent methanol adds water until 10kg, obtains the combination sterilization composition of prevention and treatment pear tree black heart together with base-material
10kg。
The compound proportion of 2 rosickyite azoles of embodiment and Flusilazole is 1: 3
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd. to produce is 98% rosickyite azoles raw medicine 1.0kg, then takesHubei Cat Er Wo biological medicine Co., LtdThe flusilazole 3.0kg of the mass fraction 95% of production is re-dubbed 4.0 kg prevention and treatment pears
Set the base-material of the combination sterilization composition of black heart；
0.4 kg of wetting dispersing agent agriculture breast 600-1,0.02 kg of thickener xanthan gum, 0.4 kg of antifreeze glycol are taken again,
0.05 kg of defoaming agent methanol adds water until 10kg, obtains the combination sterilization composition of prevention and treatment pear tree black heart together with base-material
10kg。
The compound proportion of 3 rosickyite azoles of embodiment and Flusilazole is 1: 4
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd. to produce is 98% rosickyite azoles raw medicine 1.0kg, then takesHubei Cat Er Wo biological medicine Co., LtdThe flusilazole 4.0kg of the mass fraction 95% of production is re-dubbed 5.0 kg prevention and treatment pears
Set the base-material of the combination sterilization composition of black heart；
0.4 kg of wetting dispersing agent agriculture breast 600-1,0.02 kg of thickener xanthan gum, 0.4 kg of antifreeze glycol are taken again,
0.05 kg of defoaming agent methanol adds water until 10kg, obtains the combination sterilization composition of prevention and treatment pear tree black heart together with base-material
10kg。
Rosickyite azoles and the bactericidal composition of Flusilazole of a kind of prevention and treatment pear tree black heart of the invention and application thereof have passed through
Specific embodiment is described, and combination sterilization composition made from 3 embodiments has fine prevention and treatment to pear tree black heart
Effect.It is corresponding to realize that those skilled in the art can use for reference the links such as the appropriate feed change of the contents of the present invention, process conditions
Other purposes, correlation change all without departing from the contents of the present invention, all similar substitutions and modifications are for this field skill
It is it will be apparent that being considered as being included within the scope of the present invention for art personnel.
Claims (3)
1. a kind of combination sterilization composition for preventing and treating pear tree black heart, it is characterised in that the composition is aqueous suspension agent, including work
Property ingredient and auxiliary agent, wherein active constituent is to be compounded using rosickyite azoles with Flusilazole as base-material, and auxiliary agent includes: wetting and dispersing
Agent, thickener, antifreezing agent and defoaming agent；
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Defoaming agent 0.4%~0.6%
Water adds to 100%；
The quality proportioning of rosickyite azoles and Flusilazole is 1: 4~4: 1 in the active constituent.
2. combination sterilization composition as described in claim 1, it is characterised in that the combination sterilization composition is aqueous suspension agent, packet
Active constituent and auxiliary agent are included, wherein active constituent is compounded using rosickyite azoles with ZEN 90160 as base-material, and auxiliary agent includes: profit
Hygroscopic water powder, thickener, antifreezing agent and defoaming agent；
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Defoaming agent 0.4%~0.6%
Water adds to 100%；
The quality proportioning of rosickyite azoles and Flusilazole is 1: 4 in the active constituent.
3. the combination sterilization composition as described in one of claim 1-3, it is characterised in that in the auxiliary agent, wetting dispersing agent is
Agriculture breast 600-1, thickener are xanthan gum, and antifreezing agent is ethylene glycol, and defoaming agent is methanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811232599.6A CN109105384A (en) | 2018-10-22 | 2018-10-22 | A kind of combination sterilization composition for preventing and treating pear tree black heart |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811232599.6A CN109105384A (en) | 2018-10-22 | 2018-10-22 | A kind of combination sterilization composition for preventing and treating pear tree black heart |
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| Publication Number | Publication Date |
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| CN109105384A true CN109105384A (en) | 2019-01-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811232599.6A Withdrawn CN109105384A (en) | 2018-10-22 | 2018-10-22 | A kind of combination sterilization composition for preventing and treating pear tree black heart |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015078216A1 (en) * | 2013-11-28 | 2015-06-04 | Rotam Agrochem International Company Limited | A method of treating fungicidal infections, fungicidal compositions and their use |
| CN106359415A (en) * | 2016-08-29 | 2017-02-01 | 广东中迅农科股份有限公司 | Pesticidal composition containing albendazole and triazole fungicide |
| CN106818802A (en) * | 2017-03-02 | 2017-06-13 | 贵州道元生物技术有限公司 | Rosickyite azoles prevents and treats the bactericide of rice sheath blight disease with hexaconazole compounding |
-
2018
- 2018-10-22 CN CN201811232599.6A patent/CN109105384A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015078216A1 (en) * | 2013-11-28 | 2015-06-04 | Rotam Agrochem International Company Limited | A method of treating fungicidal infections, fungicidal compositions and their use |
| CN106359415A (en) * | 2016-08-29 | 2017-02-01 | 广东中迅农科股份有限公司 | Pesticidal composition containing albendazole and triazole fungicide |
| CN106818802A (en) * | 2017-03-02 | 2017-06-13 | 贵州道元生物技术有限公司 | Rosickyite azoles prevents and treats the bactericide of rice sheath blight disease with hexaconazole compounding |
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