CN102835399A - Metrafenone-containing bactericidal composition - Google Patents
Metrafenone-containing bactericidal composition Download PDFInfo
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Abstract
The invention discloses a metrafenone-containing bactericidal composition, which consists of a first active ingredient and a second active ingredient, wherein the first active ingredient is metrafenone; the second active ingredient is strobilurin fungicide which is selected from azoxystrobin, (E)-2-[2-(2, 5-dimethyl phenoxyl toluene)-benzyl]-3-methoxy methyl acrylate and coumoxystrobin; and the weight ratio of the first active ingredient to the second active ingredient is 1:10-20:1, preferably 1:5-10:1. The composition can be prepared into agriculturally permissible formulations such as water dispersible granule, wettable powder, suspension and dispersible oil suspension. The metrafenone-containing bactericidal composition has the advantages of rational composition, good bactericidal effect and low medication cost; the activity and the bactericidal effect of the metrafenone-containing bactericidal composition are not simple superposition of activity of each component; and compared with the conventional single preparation, the metrafenone-containing bactericidal composition has an obvious bactericidal effect and an obvious synergistic effect, is pollution-free for crops, has high safety, meets the safety requirements of agricultural preparations and has an obvious prevention and treatment effect on powdery mildew, downy mildew, epidemic diseases and anthracnose of the crops.
Description
Technical field
The present invention relates to a kind of bactericidal composition that contains metrafenone, particularly a kind of bactericidal composition of being made up of metrafenone and methoxy acrylic bactericide belongs to technical field of compounded pesticide.
Background technology
Metrafenone, English common name: Metrafenone, chemical name: (3-bromo-6-methoxyl group-2-aminomethyl phenyl)-(2; 3; 4-trimethoxy-6-aminomethyl phenyl) ketone is the agricultural fungicidal agent by BASF AG's exploitation, has protection, treatment and inhibition to produce the effect of spore to all kinds of crop powdery mildews.
The methoxy acrylic acid series bactericidal agent is a bactericide kind newly developed in recent years, is a big milestone in the bactericide field.Methoxy acrylic bactericide has unique mechanism of action; The active group of this series bactericidal agent is methoxy acrylic acid (ester/acid amides); Mainly act on the cytochrome b el compound in the mitochondrial respiratory chain of fungi, thereby stop the electronics transmission to suppress conk.Have protection, treat, root out, infiltration, systemic activity, and can be in plant corpus, degraded very soon in soil and the water.Although this series bactericidal agent mechanism of action is unique, the same with other bactericide, methoxy acrylic bactericide also is unable to escape the misfortune of resistance, and use cost is high, promotes the use of so limited the large tracts of land of methoxy acrylic acid series bactericidal agent.
Introduce several methoxy acrylic acid series bactericidal agent kinds below.
Fluoxastrobin, other Chinese name: the Amici reaches, English common name: azoxystrobin, chemical name: (E) 2-[2-[6-(2-cyano-benzene oxygen) pyrimidine-4-base oxygen] phenyl]-3-methoxy-methyl acrylate.Efficiently, wide spectrum; Almost can prevent and treat all fungal diseases; Powdery mildew, rust, glume blight, net blotch, downy mildew, rice blast etc. all there are good activity, have protection, root out, infiltration and systemic action, can suppress the sprouting of spore and the growth of mycelia.Can be used for cauline leaf processing, seed treatment, also can carry out soil treatment.Suitable crop is cereal, paddy rice, grape, potato, vegetables, fruit tree, beans and other crop etc.
ZJ0712; Chemical name: (E)-[2-(2 for 2-; 5-dimethyl phenoxy toluene)-phenyl]-the 3-methoxy-methyl acrylate, ZJ0712 is that bactericidal activity is higher by wide spectrum, the systemic fungicide of the exploitation of Zhejiang chemical research institute; Have protection and therapeutic action concurrently, can be used for preventing and treating diseases such as powdery mildew, downy mildew, anthracnose.
SYP-3375, English common name: coumoxystrobin, chemical name: (E)-2-(2-((3-butyl-4-methyl-coumarin-7-Ji-oxygen base) methyl) phenyl)-3-methoxy-methyl acrylate.Have protection and therapeutic action; Be a kind of disinfectant use in agriculture of high-efficiency broad spectrum, to by mastigomycetes, combine bacterium, sac fungi, basidiomycetes, and the plant disease that causes of imperfect fungus such as sclerotinia rot of colza, apple valsa canker, rice blast, sigatoka, alternaria leaf spot of apple etc. good preventive effect is arranged.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, is difficult for developing immunity to drugs, to the agricultural bactericidal composition of crop safety.
Another object of the present invention is to provide the application of above-mentioned composition on control crop powdery mildew, downy mildew, eqpidemic disease, anthracnose.
In order to overcome the defective of existing single preparation, technical scheme of the present invention is to solve like this:
A kind of bactericidal composition that contains metrafenone is made up of first active component and second active component, wherein,
(1) first active component is a metrafenone;
(2) second active components are methoxy acrylic bactericide, and said methoxy acrylic bactericide is preferably from Fluoxastrobin, ZJ0712, SYP-3375;
The weight ratio of first active component and second active component is 1:10～20:1, is preferably 1:5～10:1, and more preferably 1:2～5:1 most preferably is 2:1.
First active component and the second active component content sum are 5%～85% of said composition total weight, are preferably 8%～70%.
But the preparation formulation that bactericidal composition of the present invention can be prepared according to method known in those skilled in the art is water dispersible granules, wetting powder, suspending agent dispersed oil suspending agent.
For water dispersible granules, those skilled in the art are familiar with the use corresponding adjuvants and accomplish the present invention very much.In dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; In disintegrant such as ammonium sulfate, urea, sucrose, the glucose one or more; In binding agent such as diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family one or more; Filler such as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To wetting powder, spendable auxiliary agent has: in dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; Filler such as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To suspending agent, spendable auxiliary agent has: among dispersant such as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate (dispersing agent NNO), the TERSPERSE 2020 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt) one or more; Alkyl phenol formaldehyde resin polyoxyethylene ether), farming breast 2201, this dish-60# (common name: Span60), Tween-60 # (common name: the polyoxyethylene sorbitan stearate), agricultural newborn 1601# (common name: triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), among the TERSPERSE 4894 one or more emulsifier such as Nongru-700 # (common name:; Among wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, the TERSPERSE 2500 one or more; In thickener such as xanthans, polyvinyl alcohol, bentonite, the aluminium-magnesium silicate one or more; In preservative such as formaldehyde, benzoic acid, the Sodium Benzoate one or more; Antifoaming agent such as organic silicon defoamer; In antifreezing agent such as ethylene glycol, propane diols, glycerine, urea, inorganic salts such as the sodium chloride one or more.
But to the dispersed oil suspending agent, spendable auxiliary agent has: among dispersant such as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate, the TERSPERSE 2020 one or more; Among the serial emulsifier of emulsifier such as BY (castor oil polyoxyethylene ether) (BY-110, BY-125, BY-140), Nongru-700 #, agricultural newborn 2201#, this dish-60#, Tween-60 #, agricultural newborn 1601#, the TERSPERSE 4894 one or more; Among wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, APES phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, the TERSPERSE 2500 one or more; In thickener such as white carbon, polyvinyl alcohol, bentonite, the aluminium-magnesium silicate one or more; In antifreezing agent such as ethylene glycol, propane diols, glycerine, urea, inorganic salts such as the sodium chloride one or more; In dispersion medium such as soybean oil, rapeseed oil, corn oil, methyl oleate, diesel oil, machine oil, the mineral oil one or more.
Embodiment
Explain that through Toxicity Determination metrafenone and Fluoxastrobin, ZJ0712, SYP-3375 are mixed to the synergistic effect of powdery mildew, downy mildew, eqpidemic disease, anthracnose below.
In order to prevent and treat crop powdery mildew, the inventor has carried out mutual composite synergy research with metrafenone and Fluoxastrobin, ZJ0712, SYP-3375, and concrete grammar is:
Subjects is powdery mildew of cucumber bacterium sphaerotheca fuliginea.Former medicine is mixed with the test medicine that needs, and test method is with reference to " the agricultural industry criteria NY/T1156.11-2008 of People's Republic of China (PRC) agricultural chemicals indoor bioassay test rule bactericide the 11st part: control cucurbits powdery mildew test pot-culture method ".At first single agent and each Mixed Pharmacy are provided with 5 variable concentrations gradients (on preliminary experiment result's basis, different with proportioning according to different medicament combinations, the concentration gradient setting is also different, and preventive effect is set by geometric progression) in 5%～90% scope.With the pure water that is added with low quantity of surfactant (Tween 80), wash and get the fresh spore that covers with on the white powder germ cucumber leaves, filter with double gauze, processing spore concentration is 1 * 10
5The suspension of individual spore/milliliter is subsequent use.Every processing 3 basins repeat for 4 times, and establish the processing that only contains solvent and surfactant and do not contain effective composition and make blank.With spore suspension 24h inoculation before chemicals treatment, soup is evenly sprayed in the blade face to all wetting, treat behind the soup natural air drying subsequent use.Potted plant cucumber natural air drying after inoculation and the chemicals treatment moves to thermostatic chamber then, under the condition of 20 ℃～24 ℃ of temperature, cultivates 7 days～10 days.Treat that the sick leaf rate of blank reaches 80% when above, the classification investigation state of an illness condition of getting a haircut everywhere, 30 leaves are investigated in every processing at least, and grade scale is:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～15% of one-piece blade area;
5 grades: lesion area accounts for 16%～25% of one-piece blade area;
7 grades: lesion area accounts for 26%～50% of one-piece blade area;
9 grades: lesion area accounts for 50%～75% of one-piece blade area;
11 grades: lesion area accounts for more than 75% of one-piece blade area.
The EC of each medicament is obtained in linear regression analysis between the probit value through preventive effect and the logarithm value of series concentration
50Value with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, is estimated the activity of confession reagent agent to germ with this.
The co-toxicity coefficient of complex preparation (CTC)>=120 shows as synergistic effect; CTC≤80 show as antagonism; 80＜CTC＜120 show as summation action.
Toxicity Determination result shows: metrafenone and Fluoxastrobin, ZJ0712 or SYP-3375 are that 1:10～20:1 uses with powdery mildew of cucumber is had virulence preferably with the weight ratio; Synergistic effect is preferably all arranged, and is that 1:5～10:1 synergy is the most remarkable especially with the weight ratio.
In order to prevent and treat the downy mildew in the agricultural production, the inventor has carried out mutual composite synergy research with metrafenone and Fluoxastrobin, ZJ0712 or SYP-3375, and concrete grammar is:
It is tested object that bacterium of downy mildew of cucumber (Pseudoperonospora cubensis) is adopted in test.Test method is with reference to " the agricultural industry criteria NY/T1156.3-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy are provided with 5 variable concentrations gradients (on preliminary experiment result's basis, different with proportioning according to different medicament combinations, the concentration gradient setting is also different, and bacteriostasis rate is set) in 5%～90% scope.Test target bacterium is to pick up from the field bacterium of downy mildew of cucumber blade of 4 leaves～6 leaf positions from up to down; Wash the Pseudoperonospora cubensis sporangium of vacuum side of blade with 4 ℃ of distilled water; Be made into suspension (concentration is controlled at every milliliter of 1 * 105～1 * 107 sporangium), deposit under 4 ℃ subsequent use; Soup is evenly sprayed in the healthy leaves back side, treat the soup natural air drying after, each is handled blade and blade goes up dorsad, by being emitted on behind the marks for treatment in the box of preserving moisture.Test is established the processing that does not contain medicament and is made blank.Be inoculated in vacuum side of blade with ready fresh sporangia suspension drop 10 μ L.4 of every blade inoculations, every processing are no less than 5 leaves.24h inoculation before processing.Ware lid on the inoculation bonnet places climatic cabinate or the humidistat of illumination is arranged, the continuous illumination in every day/dark 12h alternately, temperature is 17 ℃～22 ℃, cultivates under the condition of relative moisture more than 90%.Look and handle and measurement of blank incidence and record scab diameter, unit is a millimeter (mm), calculates control efficiency.The EC of each medicament is obtained in linear regression analysis between the probit value through control efficiency and the logarithm value of series concentration
50Value with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, is estimated the activity of confession reagent agent to germ with this.
The co-toxicity coefficient of complex preparation (CTC)>=120 shows as synergistic effect; CTC≤80 show as antagonism; 80＜CTC＜120 show as summation action.
Toxicity Determination result shows: metrafenone and Fluoxastrobin, ZJ0712 or SYP-3375 are that 1:10～20:1 uses with cucumber downy mildew is had virulence preferably with the weight ratio; Synergistic effect is preferably all arranged, and is that 1:5～10:1 synergy is the most remarkable especially with the weight ratio.
In order to prevent and treat eqpidemic disease, the anthracnose in the agricultural production, the inventor has carried out mutual composite synergy research with metrafenone and Fluoxastrobin, ZJ0712, SYP-3375, and concrete grammar is:
It is tested object that tomato early blight bacterium (Alternaria solani), apple anthrax bacteria (Gleosporium fructigenum Berk) are adopted in test.Former medicine is mixed with the test medicine that needs, and test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy are provided with 5 variable concentrations gradients (on preliminary experiment result's basis, different with proportioning according to different medicament combinations, the concentration setting is also different, and bacteriostasis rate is set) in 5%～90% scope.If the clear water contrast repeats 3 times.Adopt the mycelial growth rate method,, under aseptic condition, use diameter to be the 5mm card punch, cut the bacterium cake, the bacterium cake is connected on the culture medium flat plate of different drug concentrations, put in 26 ℃ of incubators and cultivate 72h with transfer needle from colony edge with cultured pathogen; The inspection colony diameter calculates the percentage that each chemicals treatment suppresses mycelial growth, and the EC of each medicament is obtained in the linear regression analysis between the probit value through inhibiting rate and the logarithm value of series concentration
50Value with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, is estimated the activity of confession reagent agent to germ with this.
The co-toxicity coefficient of complex preparation (CTC)>=120 shows as synergistic effect; CTC≤80 show as antagonism; 80＜CTC＜120 show as summation action.
Toxicity Determination result shows: metrafenone and Fluoxastrobin, ZJ0712 or SYP-3375 are that 1:10～20:1 uses with early blight of tomato, apple anthracnose are had virulence preferably with the weight ratio; Synergistic effect is preferably all arranged, and is that 1:5～10:1 synergy is the most remarkable especially with the weight ratio.
Below in conjunction with embodiment content of the present invention is described further.
FORMULATION EXAMPLE 1
Take by weighing 80% metrafenone, 5% Fluoxastrobin, 2% TERSPERSE 2700 (polycarboxylate, U.S. Huntsman Corporation produces), 1% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2% K-12 (lauryl sodium sulfate), 1% carboxymethyl cellulose, 2% urea, diatomite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 85% metrafenone azoxystrobin water dispersible granule.
FORMULATION EXAMPLE 2
Take by weighing 5% metrafenone, 50% ZJ0712,3% TERSPERSE 2700,2% dispersing agent NNO, 3% Nekal BX, 2%K-12,3% diatomite, 5% glucose, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 55% metrafenone ZJ0712 water dispersible granules.
FORMULATION EXAMPLE 3
Take by weighing 30% metrafenone, 30% SYP-3375,3% TERSPERSE 2700,3% dispersing agent NNO, 2% Nekal BX, 1%K-12,1% carboxymethyl cellulose, 3% urea, diatomite adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is promptly mixed, ultra micro comminution by gas stream, mixing, granulation step are produced 60% metrafenone SYP-3375 water dispersible granules.
FORMULATION EXAMPLE 4
Take by weighing 50% metrafenone, 2.5% Fluoxastrobin, 4% calcium lignosulfonate, 3% TERSPERSE 2700,2% Nekal BX, 2%K-12,3% white carbon black, fine particle calcium carbonate adds to 100% weight portion.Above-mentioned raw materials makes 52.5% metrafenone Fluoxastrobin wetting powder through mixing behind the comminution by gas stream.
FORMULATION EXAMPLE 5
Take by weighing 15% metrafenone, 30% ZJ0712,4% Morwet D-425 (alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO, 2% Nekal BX, 2%K-12,3% white carbon black, kaolin adds to 100% weight portion.Above-mentioned raw materials makes 45% metrafenone ZJ0712 wetting powder through mixing behind the comminution by gas stream.
FORMULATION EXAMPLE 6
Take by weighing 35% metrafenone, 7% SYP-3375,5% calcium lignosulfonate, 2% Nekal BX, 2%K-12,5% white carbon black, kaolin and add to 100% weight portion.Above-mentioned raw materials makes 42% metrafenone SYP-3375 wetting powder through mixing behind the comminution by gas stream.
FORMULATION EXAMPLE 7
Take by weighing 21% metrafenone, 7% Fluoxastrobin, 4% TERSPERSE 4894 (U.S. Huntsman Corporation product), 1.5% TERSPERSE 2500 (U.S. Huntsman Corporation product), 2% TERSPERSE 2020 (U.S. Huntsman Corporation product), 1% aluminium-magnesium silicate, 0.2% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry article company produces), deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 28% metrafenone Fluoxastrobin suspending agent behind the sand mill sand milling.
FORMULATION EXAMPLE 8
Take by weighing 6% metrafenone, 30% ZJ0712,5%TERSPERSE 4894,2%TERSPERSE 2500,3%TERSPERSE 2020,0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 36% metrafenone ZJ0712 suspending agent behind the sand mill sand milling.
FORMULATION EXAMPLE 9
Take by weighing 20% metrafenone, 10% SYP-3375,2%TERSPERSE 2020,2% this dish-60#, 2% Tween-60 #, 1%TERSPERSE 2500,0.5% white carbon, 0.3% xanthans, 5% ethylene glycol, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 30% metrafenone SYP-3375 suspending agent behind the sand mill sand milling.
FORMULATION EXAMPLE 10
Take by weighing 2% metrafenone, 3% Fluoxastrobin, 2% dispersing agent NNO, 3% agricultural newborn 2201#, 10% BY-110,1%TERSPERSE 2500,1% aluminium-magnesium silicate, 3% glycerine, soybean oil and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, but with making 5% metrafenone Fluoxastrobin dispersed oil suspending agent behind the sand mill sand milling.
FORMULATION EXAMPLE 11
Take by weighing 32% metrafenone, 8% ZJ0712,1.5%TERSPERSE 2020,3% this dish-60#, 3% Tween-60 #, 8% BY-110,1%TERSPERSE 2500,0.5% white carbon, 5% propane diols, methyl oleate and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, but with making 40% metrafenone ZJ0712 dispersed oil suspending agent behind the sand mill sand milling.
FORMULATION EXAMPLE 12
Take by weighing 6% metrafenone, 18% SYP-3375,1% dispersing agent NNO, 5% BY-125,3% this dish-60#, 3% Tween-60 #, 4% agricultural newborn 1601#, 1% phenethyl phenol polyethenoxy base ether phosphate, 0.5% bentonite, 5% glycerine, rapeseed oil and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, but with making 24% metrafenone SYP-3375 dispersed oil suspending agent behind the sand mill sand milling.
Biological Examples 1: control wheat powdery mildew field trial
2012 in Shaanxi Province Ankang City carried out FORMULATION EXAMPLE 7 (28% metrafenone Fluoxastrobin suspending agent), FORMULATION EXAMPLE 5 (45% metrafenone ZJ0712 wetting powder), FORMULATION EXAMPLE 3 (60% metrafenone SYP-3375 water dispersible granules) control wheat powdery mildew field trial, verified that these several kinds of medicaments are to the control efficiency of wheat powdery mildew and to the safety of wheat.Trial crops is a wheat, and controlling object is wheat powdery mildew (Blumeria graminis).Test is located at the Ankang City Hanyin County, and the experimental field physical features is smooth, and soil is loam, and fertility is better-than-average, pH value 7.0, and the duration of test rich water quality management is medium.Test medicine and dosage see table 5 for details.Other establishes blank, and every processing 4 times repeats, every sub-district 33 ㎡, and totally 32 sub-districts, district's group is arranged at random.Adopt conventional spray-on process, mu dispenser liquid 45kg evenly is sprayed at leave dual sides.Prevent and treat 1 time at wheat booting period and each spraying of flowering initial stage, dispenser is 2 times altogether.
Investigation and statistical method were respectively investigated 1 time, and were investigated altogether 2 times in the 7th day and the 14th day after the 2nd dispenser.Adopt five point samplings at random, get 20 strains at every, investigate last 3 leaves (having boot leaf then to comprise boot leaf) of every strain, write down total number of sheets, the sick number of sheets, sick leaf rate,, put down in writing the sick numbers of sheets at different levels, calculate disease index and preventive effect according to 9 grades of grade scales of wheat powdery mildew.Behind each spray medicine behind the 1st day and the medicine some days, each handled the influence to fruit and blade to observe medicament.
The wheat powdery mildew grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-15% of whole leaf area;
5 grades: lesion area accounts for the 16%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 51% of whole leaf area.
Calculate disease index and control efficiency according to the record incidence.
Disease index=[∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigation total leaf number * 9)] * 100
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] * 100.
Field test results shows; 28% metrafenone Fluoxastrobin suspending agent, 45% metrafenone ZJ0712 wetting powder, 60% metrafenone SYP-3375 water dispersible granules have good control efficiency to wheat powdery mildew; Press 10g/667 ㎡, 10g/667 ㎡, 6g/667 ㎡ consumption respectively; To wheat powdery mildew for the second time behind the medicine 7 days preventive effect be respectively 89.6%, 90.9%, 93.5%; The 14th day preventive effect is respectively 86.5%, 87.4%, 85.1%; The preventive effect that obviously is superior to 20% metrafenone suspending agent 20g/667 ㎡, 25% Fluoxastrobin suspending agent 50g/667 ㎡, 10% ZJ0712 suspending agent 60g/667 ㎡, 20% SYP-3375 suspending agent 30g/667 ㎡, difference be (seeing table 5) extremely significantly.
To the security survey of wheat, to observe in some days behind the 1st day and the medicine behind the spray medicine, each test is handled not to be had the poisoning phenomenon to wheat leaf blade, flower, fringe and takes place.
After result of the test shows that metrafenone and Fluoxastrobin, ZJ0712, SYP-3375 are mixed; Obviously improved the control efficiency to wheat powdery mildew, synergistic effect is obvious, has reduced dosage and drug cost; To wheat growth safety, be the desirable medicament of control wheat powdery mildew.
Biological Examples 2: control cucumber downy mildew field trial
Carried out FORMULATION EXAMPLE 7 (28% metrafenone Fluoxastrobin suspending agent), FORMULATION EXAMPLE 11 (but 40% metrafenone ZJ0712 dispersed oil suspending agent), FORMULATION EXAMPLE 9 (30% metrafenone SYP-3375 suspending agent) control cucumber downy mildew field trial in 2012 in the Baiyun District, Guangzhou City, verified that these several kinds of medicaments are to the control efficiency of cucumber downy mildew and to the safety of cucumber.Test method is with reference to " pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating cucumber downy mildew GB/T 17980.26-2000 ".Trial crops is a cucumber, and controlling object is cucumber downy mildew (Pseudoperonospora cubensis).Test is located at Baiyun District Taihe county town, soil pH value 6.5, and fertility is better, the content of organic matter 1.52%, experimental field cultivation condition uniformity.Test medicine and dosage see table 6 for details.Other establishes blank, and every processing 4 times repeats, every sub-district area 45 ㎡, and totally 32 sub-districts, district's group is arranged at random.Adopt conventional spray-on process, mu dispenser liquid 60kg.Cucumber is in and comes into bloom during dispenser for the first time, and dispenser is 2 times altogether, 7 days at interval.
Investigation and statistical method:
Behind first time medicine 7 days with dispenser for the second time after carried out the disease index investigation in 14 days, 5 points are investigated in each sub-district, every some investigation 5 strain cucumber are write down total number of sheets, the sick number of sheets at different levels and sick progression, calculate disease index.
Grade scale is:
0 grade, no scab;
1 grade, lesion area accounts for below 5% of whole leaf area;
3 grades, lesion area accounts for the 6%-10% of whole leaf area;
5 grades, lesion area accounts for the 11%-20% of whole leaf area;
7 grades, lesion area accounts for the 21%-50% of whole leaf area;
9 grades, lesion area accounts for more than 50% of whole leaf area.
Disease index and control efficiency computational methods:
Disease index=Σ (the sick numbers of sheets at different levels * relative level numerical value)/investigate total number of sheets * 9 * 100%
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] * 100
Security survey: sprayed behind the medicine the 1st day at every turn and medicine after some days, each handles the influence to cucumber to observe medicament.
Field test results shows: but 28% metrafenone Fluoxastrobin suspending agent 15g/667 ㎡, 40% metrafenone ZJ0712 dispersed oil suspending agent 10g/667 ㎡, 30% metrafenone SYP-3375 suspending agent 15g/667 ㎡; To cucumber downy mildew for the first time behind the medicine 7 days preventive effect be respectively 78.30%, 76.7%, 77.8%, to cucumber downy mildew for the second time behind the medicine 14 days preventive effect be respectively 87.0%, 84.8%, 83.5%.Obviously be superior to 20% metrafenone suspending agent 30g/667 ㎡; 25% Fluoxastrobin suspending agent 50g/667 ㎡; 10% ZJ0712 suspending agent 60g/667 ㎡, 20% SYP-3375 suspending agent 30g/667 ㎡ shows good quick-acting and lasting effect to the preventive effect (seeing table 6) of cucumber downy mildew.
Duration of test is observed, and each chemicals treatment does not produce the poisoning phenomenon to cucumber.
Result of the test has obviously improved the control efficiency to cucumber downy mildew after showing that metrafenone and Fluoxastrobin, ZJ0712, SYP-3375 are mixed, and has reduced dosage and drug cost, to cucumber growth safety, is the desirable medicament of control cucumber downy mildew.
Visible from above embodiment; The first active component metrafenone and the second active component Fluoxastrobin, ZJ0712, SYP-3375 make up composite; But can be made into water dispersible granules, wetting powder, suspending agent dispersed oil suspending agent formulation, powdery mildew of cucumber, wheat powdery mildew, cucumber downy mildew, early blight of tomato, apple anthracnose are had remarkable prevention effect, have that dosage is few, the control efficiency advantages of higher; Practiced thrift drug cost and dosage, and good to crop safety.Those skilled in the art can learn obviously that this based composition also can be used as the control of other crop powdery mildew, downy mildew, eqpidemic disease, anthracnose, therefore produce to have wide practical use.
In sum, composition of the present invention is to adopt two kinds of active component compound schemes, and its activity and bactericidal effect are not the simple superposition of each composition activity; Compare with existing single preparation; Except that having significant bactericidal effect, and significant synergistic effect is arranged, pollution-free to crop; Safety is good, meets the security requirement of pesticidal preparations.
Claims (10)
1. bactericidal composition that contains metrafenone is characterized in that active component is made up of first active component and second active component,
(1) first active component is a metrafenone;
(2) second active components are methoxy acrylic bactericide;
The weight ratio of first active component and second active component is 1:10～20:1.
2. according to the said bactericidal composition that contains metrafenone of claim 1, it is characterized in that: methoxy acrylic bactericide is selected from Fluoxastrobin, ZJ0712, SYP-3375.
3. according to the said bactericidal composition that contains metrafenone of claim 2, it is characterized in that: the weight ratio of first active component and second active component is 1:5～10:1.
4. according to the said bactericidal composition that contains metrafenone of claim 3, it is characterized in that: the weight ratio of first active component and second active component is 1:2～5:1.
5. according to the said bactericidal composition that contains metrafenone of claim 4, it is characterized in that: the weight ratio of first active component and second active component is 2:1.
6. according to claim 1 or the 2 said bactericidal compositions that contain metrafenone, it is characterized in that: first active component and the second active component content sum are 5%～85% of said composition total weight.
7. according to the said bactericidal composition that contains metrafenone of claim 6, it is characterized in that: first active component and the second active component content sum are 8%～70% of said composition total weight.
8. according to claim 1 or the 2 said bactericidal compositions that contain metrafenone, it is characterized in that: but said combination dosage form is water dispersible granules, wetting powder, suspending agent or dispersed oil suspending agent.
9. one of any said application of bactericidal composition in control crop powdery mildew, downy mildew, eqpidemic disease, anthracnose that contains metrafenone of claim 1 to 5.
10. according to the said application of claim 9, it is characterized in that: the bactericidal composition that contains metrafenone is used to prevent and treat powdery mildew of cucumber, wheat powdery mildew, cucumber downy mildew, early blight of tomato, apple anthracnose.
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| CN104115855A (en) * | 2013-04-25 | 2014-10-29 | 陕西美邦农药有限公司 | Efficient sterilization composition containing SYP-7017 |
| CN106259373A (en) * | 2016-08-16 | 2017-01-04 | 李祥英 | A kind of containing fluoxastrobin with the composition pesticide of metrafenone |
| CN108077287A (en) * | 2018-01-12 | 2018-05-29 | 广东广康生化科技股份有限公司 | Bactericidal composition and its application comprising folpet |
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| CN108077287A (en) * | 2018-01-12 | 2018-05-29 | 广东广康生化科技股份有限公司 | Bactericidal composition and its application comprising folpet |
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| CN102835399B (en) | 2014-04-30 |
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