CN106434499A - Actinomycete strain KN37 and application thereof - Google Patents
Actinomycete strain KN37 and application thereof Download PDFInfo
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- CN106434499A CN106434499A CN201611143553.8A CN201611143553A CN106434499A CN 106434499 A CN106434499 A CN 106434499A CN 201611143553 A CN201611143553 A CN 201611143553A CN 106434499 A CN106434499 A CN 106434499A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/06—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using actinomycetales
Abstract
The invention belongs to the technical field of biology engineering and particularly discloses an actinomycete strain KN37 and an application thereof. Through microscopic observation and 16S rDNA sequence analysis, the condition that the separated actinomycete strain KN37 belongs to Streptomyces is identified, and the preservation number of the actinomycete strain KN37 is CGMCC No.13160. A fermentation broth of the strain KN37 has a better inhibition effect on multiple phytopathogens such as Botrytis cinerea, Alternaria solani, Fusarium oxysporum, Fulvia fulva and the like and can be used for preparing microbial agents for controlling crop diseases.
Description
Technical field
The invention belongs to technical field of bioengineering, it is related to microorganism and its application and in particular to one kind is under the jurisdiction of strepto-
The actinomycetes strain KN37 of Pseudomonas, and it is in the application of suppression multiple kinds of crops pathogen.
Background technology
The superior natural conditions in Xinjiang make it have inborn advantage in agricultural product producer face.Planting industry is Agriculture in Xinjiang
In major industry, account for the 76% of the general production value of agriculture, be to increase the main source of agricultural incomes.Xinjiang long-term cropping mainly includes
Grain class, oil Class, Cotton Gossypii class, greengrocery, melon and fruit class and other industrial crops.However, plant disease seriously threatens farming
The normal production of thing, almost every kind of crops all endangered by multiple pathogenic fungi.According to statistics, the whole world is every year because of phytopathy
Up to 2,000,000,000,000 dollars of the economic loss that evil causes, wherein only one annual economic loss of vegetable crop just can reach 2000
Hundred million dollars.
China is also millions of because of the loss that plant disease causes every year, especially Xinjiang region.As cotton in Xinjiang
Every year because disease loses about 360,000 tons of gined cotton, about 7,000,000,000 yuan of market price.Plant disease causes the loss of Xinjiang region processing tomato same
Sample is huge, causes economic loss to reach as many as 3,000,000,000 yuan every year.Therefore, the preventing and treating of plant disease is all that puzzlement agricultural is raw all the time
The a great problem producing.
In recent years, chemical pesticide a large amount of using leading to that the export of farm produce is limited, Drug resistance, environmental pollution the problems such as product
Raw.Therefore, exploitation new and effective, wide spectrum, low toxicity, the antibacterial (microbial reagent) of environmental protection are always the difficulty of current pesticide research
Point and focus.
Content of the invention
The present invention provides a kind of actinomycetes strain KN37, and the fermentation liquid of bacterial strain KN37 can suppress multiple kinds of crops pathogen,
Can be used for developing into new and effective, wide spectrum, low toxicity, the microbial bactericide of environmental protection.
For achieving the above object, the present invention adopts the technical scheme that：
A kind of actinomycetes strain KN37, is that inventor separates the one kind obtaining in the soil that Kanasi in Xinjiang Uighur Autonomous gathers
Actinomycetes.The preservation information of this bacterial strain (biomaterial) is：
Title：Streptomyces sp.KN37, hereinafter referred to as KN37；
Deposit number：CGMCC No.13160；
Depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC)；
The preservation time：On October 27th, 2016.
The present invention is classified to bacterial strain KN37 identifying by 16S rDNA sequence method.Extract the DNA of this bacterium, amplification
The sequence of 16S rDNA.Can with the streptomycete phylogenetic tree that effective publication kind 16S rDNA sequence construct has gone out from bacterial strain KN37
To know, bacterial strain KN37 belongs to streptomyces.
Further, The present invention gives the seed culture medium of bacterial strain KN37 and its condition of culture.Its condition of culture is：?
28 DEG C, under the conditions of 120rpm, shaking table culture 36h in the medium；Described culture medium consists of：Peptone 10g/L, yeast extract
2g/L, acid hydrolyzed casein 2g/L, sodium chloride 6g/L, pH value 7.4.
Meanwhile, the present invention is classified to bacterial strain KN37 identifying herein in connection with morphological feature.Bacterial strain KN37 is seeded in
In culture medium, the morphological feature presenting is：Bacterium colony villiform, the color of bacterium colony is white, and substrate mycelium is no in the medium
Tabula, do not rupture, aerial hyphae grows vigorous, spore cylindricality, smooth surface, does not produce soluble pigment.
In conjunction with the qualification result of strain morphology observation, cultural characters and 16S rDNA, actinomycetes strain of the present invention
KN37 is under the jurisdiction of streptomyces, thus present patent application inventor is named as Streptomyces sp.KN37.
On the other hand, the present invention gives described actinomycetes strain KN37 in the use producing broad spectrum antibiotic activity material
On the way.
Described broad spectrum antibiotic activity material derives from fermentation liquid after liquid fermentation and culture for the bacterial strain KN37, described fermentation training
Foster condition is：Shaking table culture 7d under the conditions of 28 DEG C, 180rpm；Culture medium consists of：1% Semen Glycines powder leaching juice 1L, glucose 10g,
Peptone 3g, sodium chloride 2g, Calcium Carbonate 2g, pH is natural.
According to test data, the fermentation liquid of described bacterial strain KN37 can suppress multiple kinds of crops pathogen.
Preferably, the fermentation liquid of described bacterial strain KN37 be used for suppressing Botrytis cinerea (Botrytis cinerea), kind
Eggplant early epidemic pathogenic bacteria (Alternaria solani), cotton-wilt fusarium (Fusarium oxysporum), Fulvia fulva
The biological activity of (Fulvia fulva).
In view of the biological activity of actinomycetes strain KN37 fermentation liquid, the fermentation liquid of described bacterial strain KN37 is prepared for preventing and treating agriculture
The microbial reagent of crop pest, i.e. antibacterial of the present invention.
Compared with prior art, the present invention at least has following beneficial effects or advantage：
(1) present invention provides a kind of new actinomycetes strain KN37.This bacterial strain can, wide spectrum, low toxicity, ring efficient as development
The biomaterial of the microbial bactericide protected, the fermentation liquid of bacterial strain KN37 can be directly used for Fungicidal substance suppression multiple kinds of crops
Pathogen.Especially, the fermentation liquid of bacterial strain KN37 is to Botrytis cinerea, tomato early blight bacterium, cotton-wilt fusarium, tomato leaf
Mould pathogenic bacteria has good inhibiting effect.
(2) present invention optimizes bacterial strain KN37 seed culture medium and its condition of culture.Its condition of culture is：28 DEG C,
Under the conditions of 120rpm, shaking table culture 36h in the medium；Described culture medium consists of：Peptone 10g/L, yeast extract 2g/L, acid
Caseinhydrolysate 2g/L, sodium chloride 6g/L, pH value 7.4.
(3) The present invention gives bacterial strain KN37 has the preparation condition of the fermentation liquid of bactericidal activity.Its fermentation culture conditions
For：Shaking table culture 7d under the conditions of 28 DEG C, 180rpm；Culture medium consists of：1% Semen Glycines powder leaching juice 1L, glucose 10g, peptone
3g, sodium chloride 2g, Calcium Carbonate 2g, pH is natural.
Brief description
Fig. 1 is the colonial morphology figure of actinomycetes strain KN37 of the present invention.
Fig. 2 is the phylogenetic tree of actinomycetes strain KN37 of the present invention and related kind 16S rDNA sequence.
Specific embodiment
Below with reference to embodiment, technical scheme is elaborated further.
Embodiment 1：The separation of bacterial strain KN37 and identification
(1) actinomycetes strain KN37 is to separate to obtain a kind of actinomycetes from the soil of Kanasi in Xinjiang Uighur Autonomous collection.Should
The seed culture condition of bacterial strain KN37 is：Under the conditions of 28 DEG C, 120rpm, shaking table culture 36h in the medium；Described culture medium
Consist of：Peptone 10g/L, yeast extract 2g/L, acid hydrolyzed casein 2g/L, sodium chloride 6g/L, pH value 7.4.
(2) taxonomic identification is carried out to actinomycetes strain KN37 using 16S rDNA gene sequencing method.
16S rDNA sequencing entrusts Shanghai Sheng Gong biological engineering limited company to carry out.Concrete sequence measurement is such as
Under：Extract genomic DNA with lysozyme Method from new fresh thalli, 16S rDNA expansion is carried out using universal primer (27F and 1492R)
Increase, PCR primer is sequenced after testing afterwards.
After measured, the 16S rDNA sequence of this bacterial strain KN37 is as follows：
ACCATGCAAGTCGAACGATGAACCACTTCGGTGGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCA
ATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATAACACACGCTCAGGCATCTGAGTGTG
TTGAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGGTAGAAGCTCACCAAGGCGACGA
CGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTG
GGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTC
TTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAA
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGTTGTGAAAG
CCCGGGGCTTAACCCCGGGTCTGCAGTCGATACGGGCAGGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTA
GCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGC
GAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGAACTAGGTGTTGGCGACA
TTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAG
GAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTG
ACATATACCGGAAAGCATCAGAGATGGTGCCCCCCTTGTGGTCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCG
TGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCCTTCGGGGTGAT
GGGGACTCACAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCT
TGGGCTGCACACGTGCTACAATGGCAGGTACAATGAGCTGCGAAGCCGCGAGGCGGAGCGAATCTCAAAAAGCCTGT
CTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATTGCTGCGG
TGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAAC
CCCTTGTGGGA
Permissible with the streptomycete phylogenetic tree (Fig. 2) that effective publication kind 16S rDNA sequence construct has gone out from bacterial strain KN37
Find out：The 16S rDNA sequence similarity of bacterial strain KN37 and reference strains between 98～99%, with Streptomyces
The Phylogenetic Relationships of kanamyceticus are the closest, and the sequence similarity between it is 99%, and this is defined with current genus
The standard that middle 16S rDNA sequence similarity is not less than 95% is consistent.But this bacterial strain is not known with any on systematic evolution tree
Strain is met, but independently becomes branch with higher evolution difference, illustrates that this bacterial strain is equal on evolving with all reference type strains
There is larger difference.
(3) identification of morphology of actinomycetes strain KN37
Bacterial strain KN37 well-grown (being shown in Table 1) in most of culture medium, bacterium colony villiform in Gao Shi culture medium, white
(Fig. 1), substrate mycelium no tabula, does not rupture, and aerial hyphae grows vigorous, spore cylindricality, smooth surface.KN37 bacterial strain is at each
In culture medium, all no soluble pigment produces.
Table 1, the cultural characteristic of bacterial strain KN37
| Culture medium | Substrate mycelium | Aerial hyphae | Colony growth situation |
| Glycerinum amyli culture medium | White | White | + |
| PDA culture medium | White | White | +++ |
| ISP4 culture medium | White | White | +++ |
| Czapek's medium | White | White | + |
| ISP5 culture medium | White | White | +++ |
| LB culture medium | Light yellow | Light yellow | +++ |
| Beef-protein medium | White | Milky | ++ |
| Bennett culture medium | White | White | +++ |
| Gause I culture medium | Light yellow | White | +++ |
Note：++ for normal growth；+ poor for upgrowth situation；+++ vigorous for growing.
The physio-biochemical characteristics of bacterial strain KN37 and utilization of carbon source situation are as follows：Bacterial strain KN37 gelatin liquefaction and nitrate reduction
Reaction is the positive；Bacterial strain KN37 can utilize glucose, D-ribose, sucrose, D- xylose, D-MANNOSE, D- galactose, Fructus rhamni (Rhamnus davurica Pall.)
Sugar, arabinose and cellulose are as sole carbon source, but can not utilize D-Fructose and inositol.
Embodiment 2：The biological activity of bacterial strain KN37 and its application
(1) inhibitory activity to pathogen for the bacterial strain KN37
Measure bacterial strain KN37 fermentation liquid using growth rate method to botrytis cinerea, tomato early blight bacterium, Fructus Lycopersici esculenti leaf mold
Pathogenic bacteria, Phytophthora infestans pathogenic bacteria, bacterial spot of tomato bacterium, cotton-wilt fusarium, verticillium dahliae, P. capsici,
Apple anthrax bacteria, cucumber anthracnose, Sclerotinia sclerotiorum, fusarium graminearum, Rhizoctonia solani Kuhn, Cabbage Leaf Spot
The suppression of multiple pathogen such as bacterium, southern corn leaf blight, tobacco brown spot pathogen, Pyricularia oryzae, pine root fungus, rhizoctonia solani is lived
Property.Result ginseng is shown in Table 2, and bacterial strain KN37 is respectively provided with good inhibition (table 2) to most pathogen.
Table 2, the inhibitory action to 18 kinds of pathogen for the bacterial strain KN37 fermentation liquid
| Pathogen | Suppression ratio % | Pathogen | Suppression ratio % |
| Botrytis cinerea | 90.26 | Verticillium dahliae | 64.88 |
| Tomato early blight bacterium | 72.66 | P. capsici | 72.07 |
| Fulvia fulva | 74.02 | Apple anthrax bacteria | 88.89 |
| Phytophthora infestans pathogenic bacteria | 85.98 | Cucumber anthracnose | 85.06 |
| Bacterial spot of tomato bacterium | 91.67 | Sclerotinia sclerotiorum | 97.85 |
| Cotton-wilt fusarium | 50.62 | Fusarium graminearum | 73.76 |
| Rhizoctonia solani Kuhn | 35.79 | Alternaria brassicae | 58.91 |
| Southern corn leaf blight | 42.67 | Tobacco brown spot pathogen | 51.32 |
| Pyricularia oryzae | 69.42 | Pine root fungus | 86.33 |
(2) prevention effect to graw mold of tomato for the bacterial strain KN37 fermentation liquid
Measure the prevention effect to graw mold of tomato for the KN37 using rules of organization, result shows referring to table 3, bacterial strain KN37 ferments
Liquid has good protective effect to graw mold of tomato.
Table 3, bacterial strain KN37 fermentation liquid is to the protection of graw mold of tomato and therapeutic effect
(3) prevention effect to tomato disease for the bacterial strain KN37 fermentation liquid
After KN37 bacterial strain fermentation liquor is applied to the Fructus Lycopersici esculenti in booth, graw mold of tomato, early blight of tomato, tomato late blight,
Leaf muld of tomato, the incidence rate of tomato bacterial disease and extent of injury bacterium significantly reduce (table 4).
Table 4, the prevention effect to tomato in plastic greenhouse disease for the bacterial strain KN37 fermentation liquid
| Disease title | Prevention effect % | Disease title | Prevention effect % |
| Gray mold | 62.72 | Early blight | 61.53 |
| Late blight | 58.43 | Leaf mold | 71.66 |
| Bacterial disease | 55.25 | Root disease | 70.84 |
Shown according to the present embodiment result, bacterial strain KN37 of the present invention through liquid fermentation and culture, in its fermentation liquid
Material containing suppression common crops pathogen, can significantly mitigate the generation of disease, be expected to be developed into a kind of new
The microbial reagent of preventing and treating corps diseases.
Above in conjunction with embodiment, further narration is done to the present invention, but the present invention has been not limited to above-mentioned embodiment,
In the ken that one skilled in the relevant art possesses, can also make on the premise of without departing from present inventive concept
Various change.
Sequence table
<110>Shihezi Univ
<120>A kind of actinomycetes strain KN37 and its application
<160>1
<210>1
<211>
<212>RNA
<213>Actinomycetes strain KN37
<400>1
ACCATGCAAGTCGAACGATGAACCACTTCGGTGGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCT
GCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATAACACACGCTCAGGCATCTGAGTGTG
TTGAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGGTAGAAGCTCACCAAGGCG
ACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGG
CAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGG
GTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTG
CCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCT
TGTCACGTCGGTTGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCAGTCGATACGGGCAGGCTAGAGTGTGGTA
GGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGAT
CTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACG
CCGTAAACGGTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCC
GCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTG
GCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATATACCGGAAAGCATCAGAGATGGTGCCCC
CCTTGTGGTCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCA
ACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCCTTCGGGGTGATGGGGACTCACAGGAGACTGCCGGGG
TCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAA
TGGCAGGTACAATGAGCTGCGAAGCCGCGAGGCGGAGCGAATCTCAAAAAGCCTGTCTCAGTTCGGATTGGGG
TCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCC
GGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGA
Claims (9)
1. a kind of actinomycetes strain KN37, this bacterial strain was preserved in Chinese microorganism strain preservation management committee on October 27th, 2016
Member's meeting common micro-organisms center, preserving number is CGMCC No.13160.
2. according to claim 1 actinomycetes strain KN37 it is characterised in that described bacterial strain KN37 belongs to streptomyces.
3. according to claim 1 actinomycetes strain KN37 it is characterised in that the morphological feature of described bacterial strain is described as：
Bacterium colony villiform, the color of bacterium colony is white, in the medium substrate mycelium no tabula, no rupture, aerial hyphae growth is vigorous,
Spore cylindricality, smooth surface, no soluble pigment produce.
4. according to claim 1 actinomycetes strain KN37 it is characterised in that the seed culture condition of described bacterial strain is：?
28 DEG C, under the conditions of 120rpm, shaking table culture 36h in the medium；Described culture medium consists of：Peptone 10g/L, yeast extract
2g/L, acid hydrolyzed casein 2g/L, sodium chloride 6g/L, pH value 7.4.
5. actinomycetes strain KN37 any one of Claims 1 to 4 is producing the purposes of broad spectrum antibiotic activity material.
6. purposes according to claim 5 is it is characterised in that described broad spectrum antibiotic activity material derives from bacterial strain KN37 warp
Fermentation liquid after liquid fermentation and culture, described fermentation culture conditions are：Shaking table culture 7d under the conditions of 28 DEG C, 180rpm；Culture
Basis set become：1% Semen Glycines powder leaching juice 1L, glucose 10g, peptone 3g, sodium chloride 2g, Calcium Carbonate 2g, pH is natural.
7. purposes according to claim 6 is it is characterised in that the fermentation liquid of described bacterial strain KN37 can suppress multiple kinds of crops
Pathogen.
8. purposes according to claim 6 is it is characterised in that the fermentation liquid of described bacterial strain KN37 is used for suppressing Fructus Vitis viniferae grey mold
Pathogenic bacteria, tomato early blight bacterium, cotton-wilt fusarium, the biological activity of Fulvia fulva.
9. purposes according to claim 6 is it is characterised in that the fermentation liquid of described bacterial strain KN37 is prepared for preventing and treating farming
The microbial reagent of thing disease.
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| CN107699526A (en) * | 2017-11-20 | 2018-02-16 | 运城学院 | One plant of actinomycetes strain for preventing and treating gray mold and its application |
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| CN114931149B (en) * | 2022-06-25 | 2024-04-16 | 石河子大学 | Axillary bud inhibition composition containing microbial inoculum and preparation method thereof |
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| CN115960788A (en) * | 2022-12-31 | 2023-04-14 | 石河子大学 | Special microbial inoculum for preventing and treating cotton verticillium wilt |
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