CN114931149B - Axillary bud inhibition composition containing microbial inoculum and preparation method thereof - Google Patents
Axillary bud inhibition composition containing microbial inoculum and preparation method thereof Download PDFInfo
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- CN114931149B CN114931149B CN202210731769.5A CN202210731769A CN114931149B CN 114931149 B CN114931149 B CN 114931149B CN 202210731769 A CN202210731769 A CN 202210731769A CN 114931149 B CN114931149 B CN 114931149B
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- streptomycete
- flumetralin
- axillary bud
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- 239000000203 mixture Substances 0.000 title claims abstract description 42
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 230000005764 inhibitory process Effects 0.000 title abstract description 23
- 239000005978 Flumetralin Substances 0.000 claims abstract description 34
- PWNAWOCHVWERAR-UHFFFAOYSA-N Flumetralin Chemical compound [O-][N+](=O)C=1C=C(C(F)(F)F)C=C([N+]([O-])=O)C=1N(CC)CC1=C(F)C=CC=C1Cl PWNAWOCHVWERAR-UHFFFAOYSA-N 0.000 claims abstract description 34
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 26
- 241001655322 Streptomycetales Species 0.000 claims abstract description 19
- 241000187747 Streptomyces Species 0.000 claims abstract description 16
- 239000004480 active ingredient Substances 0.000 claims abstract description 11
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- 230000002401 inhibitory effect Effects 0.000 claims description 21
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- 239000000843 powder Substances 0.000 claims description 15
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
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- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 description 2
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- NLKUPINTOLSSLD-UHFFFAOYSA-L calcium;4-(1-oxidopropylidene)-3,5-dioxocyclohexane-1-carboxylate Chemical compound [Ca+2].CCC([O-])=C1C(=O)CC(C([O-])=O)CC1=O NLKUPINTOLSSLD-UHFFFAOYSA-L 0.000 description 2
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- 150000003852 triazoles Chemical class 0.000 description 2
- ZMYFCFLJBGAQRS-IRXDYDNUSA-N (2R,3S)-epoxiconazole Chemical compound C1=CC(F)=CC=C1[C@@]1(CN2N=CN=C2)[C@H](C=2C(=CC=CC=2)Cl)O1 ZMYFCFLJBGAQRS-IRXDYDNUSA-N 0.000 description 1
- STMIIPIFODONDC-UHFFFAOYSA-N 2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol-1-yl)hexan-2-ol Chemical compound C=1C=C(Cl)C=C(Cl)C=1C(O)(CCCC)CN1C=NC=N1 STMIIPIFODONDC-UHFFFAOYSA-N 0.000 description 1
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- 241000233866 Fungi Species 0.000 description 1
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- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
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- 239000003995 emulsifying agent Substances 0.000 description 1
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- 239000000945 filler Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000007954 growth retardant Substances 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
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- 239000007788 liquid Substances 0.000 description 1
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- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000002373 plant growth inhibitor Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- STJLVHWMYQXCPB-UHFFFAOYSA-N propiconazole Chemical compound O1C(CCC)COC1(C=1C(=CC(Cl)=CC=1)Cl)CN1N=CN=C1 STJLVHWMYQXCPB-UHFFFAOYSA-N 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
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- 239000002562 thickening agent Substances 0.000 description 1
- BAZVSMNPJJMILC-UHFFFAOYSA-N triadimenol Chemical compound C1=NC=NN1C(C(O)C(C)(C)C)OC1=CC=C(Cl)C=C1 BAZVSMNPJJMILC-UHFFFAOYSA-N 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- 239000004562 water dispersible granule Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Agronomy & Crop Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
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Abstract
The invention provides an axillary bud inhibition composition containing a microbial inoculum and a preparation method thereof, wherein the active ingredients of the composition comprise streptomycete KN37 and flumetralin; the ratio of the streptomycete KN37 to the flumetralin is 1:10-1:40 in terms of mass ratio; the total concentration of the active bacteria of the streptomyces KN37 is 1 multiplied by 10 10 ～1×10 12 cfu/mL. The invention combines streptomycete KN37 and fluorineAfter the mepiquat chloride is compounded, the synergistic effect is shown in the axillary bud inhibition, especially the axillary bud inhibition of tobacco, and no phytotoxicity is generated to the applied crops.
Description
Technical Field
The invention belongs to the technical field of pesticide compounding, and relates to an axillary bud inhibition composition containing a microbial inoculum and a preparation method thereof.
Background
Each leaf axillary of tobacco has axillary buds, and all axillary buds can germinate into branches and bloom. After topping, the top advantages are relieved, axillary buds germinate rapidly, a large amount of nutrients are consumed, and the yield and quality of tobacco leaves are affected. Topping and bud picking are important measures for improving the yield and the value of tobacco leaves, and traditional manual bud picking is labor-consuming and troublesome. The topping and bud picking are not timely and thorough, the smoke right clusters and fireworks Man Tian are easy to cause, and diseases are easy to be infected.
The capsicum is the second big vegetable crop in China, and the robust capsicum seedlings are the basis for mass production. The high temperature, rainy and high humidity environment conditions are easy to cause the hive seedling to grow excessively, the formation of the high-foot seedling, the plant growth vigor after field planting is weak, the plant is easy to lodge and is not easy to survive. Plant overgrowth is mainly due to the excessive elongation of the hypocotyl, and the influencing factors mainly comprise environmental factors and 2 aspects of endogenous hormones. The plant growth regulator can effectively prevent pepper seedlings from overgrowth and improve the quality of the pepper seedlings.
The flumetralin is a typical plant growth inhibitor, can inhibit division and growth of cells in axillary buds after entering a plant body, so as to inhibit the axillary buds from sprouting, dwarf plants, thicken stalks, thicken petioles, increase chlorophyll and strengthen photosynthesis, and is generally used as a tobacco bud inhibitor. However, the use of flumetralin alone causes phytotoxicity problems such as malformation, insufficient development, mesophyll necrosis, folding of the petiole of the middle/upper leaves, rooting disorders of adventitious roots, necrosis, and the like of tender leaves of the upper node. More seriously, the wounds on the base of the petiole caused by phytotoxicity can induce diseases such as empty heart disease, sclerotinia sclerotiorum, gray mold and the like, and adversely affect the yield and quality of tobacco leaves.
At present, a new preparation is developed by compounding the flumetralin so as to improve the quality of the preparation and improve the drug effect. CN108812661B discloses the application of a composition containing flumetralin and triazole fungicide in crop shoot control and bud inhibition, the composition is composed of one of flumetralin as a first active ingredient, triadimenol as a second active ingredient, hexaconazole, propiconazole and epoxiconazole as triazole fungicide, and an auxiliary agent. CN107439561B discloses a tobacco axillary bud inhibitor and a tobacco axillary bud inhibition method, the tobacco axillary bud inhibitor contains flumetralin and prohexadione calcium as active ingredients, the combination of flumetralin and prohexadione calcium shows synergistic tobacco axillary bud inhibition effect, germination and growth of tobacco axillary buds are inhibited for a long time at low application dose, and obvious phytotoxicity to tobacco stems and leaves is not observed.
The purpose of improving the drug effect is achieved by the mode of combining the flumetralin and the bactericide or combining the flumetralin and the plant growth retardant. At present, a composition for compounding flumetralin and a microbial inoculum is not known, and the beneficial effects generated by compounding flumetralin and microbial inoculum for plant growth regulation cannot be known by the skilled in the art.
Disclosure of Invention
At present, no report on the compounding of the flumetralin and the streptomyces KN37 for regulating the plant growth is found.
Based on the above, the invention provides an axillary bud inhibiting composition containing a microbial inoculum, wherein the active ingredients of the axillary bud inhibiting composition comprise streptomyces KN37 and flumetralin; the ratio of the streptomycete KN37 to the flumetralin is 1:10-1:40 in terms of mass ratio; the concentration of the active bacteria of the streptomycete KN37 is 1 multiplied by 10 10 ～1×10 12 cfu/g。
As one of the preferred embodiments of the technical scheme, the ratio of the streptomycete KN37 to the flumetralin is 1:20-1:34 in terms of mass ratio. Within the range of the proportion, the two components show excellent synergistic effect.
As one of the preferred embodiments of the technical scheme, the ratio of the streptomycete KN37 to the flumetralin is 1:28 in terms of mass ratio. Within the range of the proportion, the two components show excellent synergistic effect.
As one of the preferred embodiments of the technical scheme, the ratio of the streptomycete KN37 to the flumetralin is 1:10-1:40 in terms of mass ratio. Within the range of the proportion, the two components show excellent synergistic effect.
Further, the preparation method of the axillary bud inhibiting composition containing the microbial inoculum provided by the invention comprises the following steps: inoculating and culturing streptomycete KN37 to obtain bacterial suspension; inoculating the bacterial suspension into fermentation culture solution, culturing, filtering and drying to obtain Streptomyces KN37 bacterial powder.
Further, in the preparation method of the axillary bud inhibition composition containing the microbial inoculum, streptomycete KN37 is inoculated and cultured by using a culture medium of Gao's No.1, and the culture conditions are as follows: shake culturing at 32deg.C at 180rpm for 36 hr.
Further, in the preparation method of the axillary bud inhibition composition containing the microbial inoculum, the ratio of the fungus suspension to the fermentation culture solution is 1:100 by mass ratio; the formula of the fermentation culture solution is as follows: 10g/L of corn starch, 10g/L of peanut cake powder, 10g/L of glucose, 3g/L, naCl g/L, caCO of peptone 3 1g/L, pH 7.2-7.4; the fermentation culture conditions are as follows: the temperature is 32 ℃, the rotating speed is 280rpm, the pH value is 7.0-7.5, the dissolved oxygen amount is 20%, and the ventilation amount is 5-7m 3 And/h, the tank pressure is 0.05-0.1KPa.
Still another object of the present invention is to provide an axillary bud inhibiting composition containing a microbial agent for regulating the growth of crops; crops include tobacco and capsicum.
According to the actual application requirement, the axillary bud inhibiting composition containing the microbial inoculum provided by the invention can be prepared into a preparation dosage form which is convenient for agricultural application, such as one of a suspending agent, a wettable powder, a water dispersible granule, an aqueous emulsion, an emulsifiable concentrate and the like according to a method known by a person skilled in the art. Those skilled in the art will readily appreciate that the formulation system contains, in addition to the active ingredient, adjuvants required for the formulation of the pesticide formulation. The auxiliary agent can be one or a mixture of a plurality of solvents, emulsifying agents, wetting agents, stabilizing agents, dispersing agents, thickening agents, pH regulators, defoamers, antifreezing agents, fillers and the like. The adjuvants of the present invention may be all known substances, such as various adjuvants commonly used in pesticide preparations, and may be changed according to different conditions, and are not particularly limited.
The agricultural composition of the present invention has at least the following advantages or features compared with the prior art
The beneficial effects are that:
compared with the prior art, the invention mainly contributes to providing the axillary bud inhibition composition containing the microbial inoculum, the preparation method thereof and the application of the composition in agriculture, wherein the active ingredients of the composition comprise flumetralin and streptomyces KN37. The invention combines the flumetralin and the streptomyces KN37, and discovers that the flumetralin can effectively regulate plant growth within the proportion range provided by the invention, and has outstanding performance in inhibiting tobacco and pepper axillary buds.
Detailed Description
The following describes the technical aspects of the present invention with reference to examples, but the present invention is not limited to the following examples.
The experimental methods and the detection methods in the following embodiments are all conventional methods unless otherwise specified; the medicaments and materials are commercially available unless specified; the index data are all conventional measurement methods unless specified. The preservation number of Streptomyces KN37 in the following examples is CGMCC No.13160.
Example 1
The present example provides a test for preparing Streptomyces KN37 powder and an axillary bud inhibiting composition containing a microbial inoculum.
And (3) inoculating streptomyces KN37 to a culture medium of Gao's No. I, and performing shake culture for 36h at the temperature of 32 ℃ and the rotating speed of 180rpm to obtain a streptomyces KN37 bacterial suspension. And (3) inoculating the bacterial suspension into a fermentation tank filled with fermentation culture solution according to the mass ratio of 1:100. The formula of the fermentation culture solution is as follows: 10g/L of corn starch, 10g/L of peanut cake powder, 10g/L of glucose, 3g/L, naCl g/L, caCO of peptone 3 1g/L, pH 7.2-7.4. The fermentation culture conditions are as follows: the temperature is 32 ℃, the rotating speed is 280rpm, the pH value is 7.0-7.5, the dissolved oxygen amount is 20%, and the ventilation amount is 5-7m 3 And/h, the tank pressure is 0.05-0.1KPa. Culturing for 4-5d, filtering, and spray drying to obtain bacterial powder containing 1×10 per gram of bacterial powder 13 -10 17 And (3) bacterial cells.
The concentration of the viable bacteria is 1 multiplied by 10 10 ～1×10 12 Mixing cfu/g of streptomycete KN37 bacterial powder with flumetralin; the ratio of the streptomycete KN37 to the flumetralin is 1:10-1:40 in terms of mass ratio.
Example 2
This example provides an assay of axillary bud inhibition in tobacco using a microbial agent-containing axillary bud inhibition composition.
In the embodiment, the tobacco variety is flue-cured tobacco K326, and the test site is Zhaotong city and town county in Yunnan province. The test was conducted with 8 treatments, 5 treatments with compositions, 2 treatments with a single dose, 1 treatment with a fresh water control, each treatment having the active ingredients shown in Table 1, composition source example 1. Each treatment was repeated 3 times and the cell area was 50m 2 100 plants of tobacco are planted in each cell, and the cells are arranged randomly. Topping up 50% of tobacco plants in the tobacco field when central flowers are opened, removing short axillary buds, taking medicine within 24 hours at a length of more than 20cm of the top leaf, wherein the amount of medicine liquid of each plant is 20mL, pouring in a cup, righting the inclined tobacco plants, and taking medicine again when the medicine is taken, wherein the medicine is taken on a sunny day. The number of buds exceeding 2cm was measured at each of 10 plants per cell at 3 points, 14d, 28d and 42d after the application, and the buds were picked up and weighed at the time of the measurement at 42d after the application, and the statistical results are shown in Table 1.
Bud inhibition rate (%) = [ control average individual bud count-treatment average individual bud count/control average individual bud count ] ×100
Fresh weight inhibition (%) = [ control average individual bud weight-treatment average individual bud weight/control average individual bud weight ] ×100
TABLE 1 tobacco axillary bud inhibiting effect of axillary bud inhibiting composition containing microbial inoculum
As can be seen from Table 1, streptomyces KN37 has a poor effect of inhibiting tobacco axillary buds, but the bud inhibition effect of Fluorozamide is greatly improved after the mixture of the Streptomyces KN37 and Fluorozamide. After the application, 14d, 28d and 42d, the bud inhibition rate of the composition is obviously improved (p is less than 0.01) compared with that of the flumetralin, and the bud inhibition rates of 94.42%, 90.46% and 83.80% are respectively reached to the maximum. After 42d of application, the fresh weight inhibition rate of the composition is remarkably improved (p is less than 0.01) compared with that of the flumetralin, and the fresh weight inhibition rate is up to 74.71%. From this, it was found that, after the combination of Streptomyces KN37 and flumetralin, the synergistic effect was exhibited by the inhibition of axillary buds, particularly of tobacco, and the occurrence of phytotoxicity problems such as malformation, insufficient development, mesophyll necrosis, folding of the stem part of the middle/upper leaf, rooting disorder of adventitious roots, necrosis, etc. of tender leaves of tobacco in the cells treated with the composition was observed after the test.
Example 3
This example provides an experiment of an axillary bud inhibiting composition containing a microbial inoculum in controlling pepper growth.
In the embodiment, the chilli variety is Zun-peppery No. 5, and the test site is Zun-Yi-city Zun-Yi-county in Guizhou province. The test consisted of 9 treatments, 5 treatments with compositions, 2 treatments with single dose, 1 positive control treatment, 1 clear water control treatment, each treatment having the active ingredients shown in table 1, composition source example 1. The active ingredients and the treatment concentrations shown in table 1 are uniformly sprayed on the leaf surfaces of the pepper seedlings in the period of two leaves and one core by using a spray pot until the leaf surfaces are completely soaked. Each treatment was repeated 3 times, 30 strains each. And measuring physiological indexes of the capsicum seedlings in seedling stages such as plant height, total chlorophyll and root cap ratio on 15 th day after spraying, and counting the early single plant yield after the plant stage. Measurements were repeated 3 times per treatment, 5 strains were randomly selected for each repetition.
TABLE 2 effect of the axillary bud inhibiting composition containing the microbial agent on the growth of Capsicum annuum
As shown in table 1, streptomyces KN37 has a poor effect of inhibiting the plant height of capsicum, but shows a certain effect of promoting the increase of the stem thickness of capsicum, and shows an effect of inhibiting the plant height of capsicum after being mixed with flumetralin. According to the combination of root-cap ratio and chlorophyll data, although the flumetralin and paclobutrazol can effectively inhibit the growth of the capsicum in the young period, the dwarfing effect of the capsicum is not uniform due to different treatment concentrations, which suggests that the application of flumetralin and paclobutrazol requires more severe control of spraying concentration. The axillary bud inhibition composition containing the microbial inoculum provided by the invention can better inhibit the overgrowth of pepper seedlings, and can improve the pepper yield to a maximum of 262.48 g/plant.
The present invention may be better implemented as described above, and the above examples are merely illustrative of preferred embodiments of the present invention and not intended to limit the scope of the present invention, and various changes and modifications made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection defined by the present invention without departing from the spirit of the design of the present invention.
Claims (3)
1. An axillary bud inhibiting composition containing a microbial inoculum is characterized in that the active ingredients comprise streptomycete KN37 bacterial powder and flumetralin; the ratio of the streptomycete KN37 bacterial powder to the flumetralin is 1:28 by mass ratio; the active bacteria concentration of the streptomycete KN37 bacteria powder is 1 multiplied by 10 10 ~1×10 12 cfu/g。
2. A preparation method of an axillary bud inhibiting composition containing a microbial inoculum is characterized in that the preparation method of the inhibiting composition is that the concentration of viable bacteria is 1 multiplied by 10 10 ~ 1×10 12 Mixing cfu/g of streptomycete KN37 bacterial powder with flumetralin; the ratio of the streptomycete KN37 bacterial powder to the flumetralin is 1:28 by mass ratio;
the preparation method of the streptomyces KN37 bacterial powder comprises the following steps: inoculating streptomycete KN37 to a culture medium of Gao's No. I, and performing shake culture for 36h at the temperature of 32 ℃ and the rotating speed of 180rpm to obtain streptomycete KN37 bacterial suspension; inoculating the bacterial suspension and the fermentation culture solution into a fermentation tank for culture in a mass ratio of 1:100; culturing for 4-5d, filtering, and spray drying to obtain Streptomyces KN37 powder; the formula of the fermentation culture solution is as follows: 10g/L of corn starch, 10g/L of peanut cake powder, 10g/L of glucose, 3g/L, naCl g/L, caCO of peptone 3 1g/L, pH 7.2-7.4; the fermentation culture conditions are as follows: the temperature is 32 ℃, the rotating speed is 280rpm, the pH value is 7.0-7.5, the dissolved oxygen amount is 20%, and the ventilation amount is 5-7m 3 And/h, the tank pressure is 0.05-0.1KPa.
3. The bacterial agent-containing axillary bud inhibiting composition of claim 1 or the bacterial agent-containing axillary bud inhibiting composition prepared by the method for preparing the bacterial agent-containing axillary bud inhibiting composition of claim 2 has an effect of inhibiting tobacco axillary buds or regulating the growth of capsicum.
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