CN110973162A - Bacillus methylotrophicus and metalaxyl-M compound granule - Google Patents
Bacillus methylotrophicus and metalaxyl-M compound granule Download PDFInfo
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- CN110973162A CN110973162A CN201911158333.6A CN201911158333A CN110973162A CN 110973162 A CN110973162 A CN 110973162A CN 201911158333 A CN201911158333 A CN 201911158333A CN 110973162 A CN110973162 A CN 110973162A
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- metalaxyl
- bacillus methylotrophicus
- compound granule
- granule according
- dispersant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
Abstract
The invention discloses a bacillus methylotrophicus and metalaxyl-M compound granule, which belongs to the technical field of biology, wherein active ingredients of the granule comprise bacillus methylotrophicus 9912 and metalaxyl-M, the granule provided by the invention is used for preventing and treating crop root rot, the bacillus methylotrophicus and metalaxyl-M compound have a synergistic effect, the lasting period is long, meanwhile, lipopeptide substances secreted by the bacillus methylotrophicus 9912 can promote the healing and growth of plant roots, and the effect is more excellent.
Description
Technical Field
The invention relates to a bacillus methylotrophicus and metalaxyl-M compound granule, belonging to the technical field of biology.
Background
The bacillus is an important biological group in the biological control research of plant diseases, has various and wide distribution types, and is an advantageous group of soil and plant micro-ecology. Bacillus methylotrophicus is a new species reported by Munusamy Madhaiyan et al in 2010, and the application of the Bacillus methylotrophicus in the aspects of sewage treatment, aquaculture, biological control, food and the like is also reported successively. The bacillus methylotrophicus is marine bacteria separated from Bohai sea mud by Shenyang application ecological research institute of Chinese academy of sciences, is named as bacillus methylotrophicus 9912, belongs to gram-positive bacilli, Bacillaceae and bacilli; the strain is bacillus capable of forming spores (endospores), exists in nature in the form of trophosome and dormant spores, has strong antagonistic action on fungal diseases such as vegetable root rot, soybean root rot, apple rot and the like by fermentation products, has no adverse effect on human and livestock, is nontoxic to environment, and is a microbial pesticide with wide application prospect.
metalaxyl-M is a ribosome RNAI synthetic inhibitor with systemic property, can be absorbed by root, stem and leaf of plant, and transferred into various organs of plant, and has high efficiency for plant diseases of vegetable, fruit tree, tobacco, oil plant, cotton and grain, etc. caused by downy mildew, phytophthora and pythium.
Root rot is very common in the planting industry, solanaceae vegetables such as crops, soybeans and cucumbers, and various traditional Chinese medicinal materials are damaged by the root rot, and the root rot has great influence on the growth of the crops because the root rot occurs in seedling stage. The root rot of crops is caused by the compound infection of various pathogenic bacteria, and the main pathogenic bacteria comprise various fusarium, rhizoctonia solani, pythium and the like.
The root rot of cucumber is a common disease of cucumber, mainly damaging roots and stems close to the ground. The disease starts to develop, the roots of the diseased plants are soaked in water and then become light brown to yellow brown and rot. The leaves will wither and droop in the middle of the day in a sunny day, and recover in the morning and at night. After several days, the leaves were unable to return to normal and died as withered leaves. After death, the roots are completely rotted, leaving only filamentous vascular bundles. Atrophy of the base of the stem is not obvious, and vascular bundles at rotten parts of diseased parts become brown and do not develop upwards, which is different from blight.
Strawberry root rot is mainly a root system disease, which starts from small lateral roots or new roots, and initially appears light red brown irregular plaques, and gradually turns dark brown. As the disease progresses, all roots rapidly die and brown. The aerial parts of the plants are yellow, browned, necrotic and curled in the outer leaf margin, and the diseased plants are in a water-deficient state and gradually grow centripetally to be withered and yellow and die of the whole plants.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the compound granule of the bacillus methylotrophicus and the metalaxyl-M, which not only has very good control effect on the root rot of cucumber and strawberry, but also has obvious effect on promoting the growth of the roots of plants by lipopeptide substances secreted by the special strain of the bacillus methylotrophicus.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a bacillus methylotrophicus and metalaxyl-M compound granule, the active ingredients of the granule comprise bacillus methylotrophicus 9912 and metalaxyl-M.
The technical scheme of the invention is further improved as follows: the weight ratio of the bacillus methylotrophicus 9912 to the metalaxyl-M is 20: 1-1: 20.
The technical scheme of the invention is further improved as follows: the weight ratio of the bacillus methylotrophicus 9912 to the metalaxyl-M is 10: 1.
The technical scheme of the invention is further improved as follows: the granules also comprise a dispersing wetting agent, an adsorbent, a filler and a disintegrating agent.
The technical scheme of the invention is further improved as follows: the content of the metalaxyl-M is 0.1-20%, the content of the dispersing wetting agent is 0.5-20%, the content of the adsorbent is 0.1-10%, the content of the disintegrating agent is 1-30%, and the content of the filler is 10-70%.
The technical scheme of the invention is further improved as follows: the dispersing and wetting agent is selected from one or more of EO-PO block ether wetting dispersant, sulfonate dispersant, polyphosphate dispersant, calcium lignosulfonate, polycarboxylate dispersant, phosphate dispersant and rosin wetting dispersant.
The technical scheme of the invention is further improved as follows: the adsorbent is selected from one or more of white carbon black, kaolin, light calcium carbonate and bentonite.
The technical scheme of the invention is further improved as follows: the disintegrating agent is selected from one or more of sodium chloride, sodium sulfate, sodium carboxymethyl starch, ammonium sulfate, urea, polyvinylpyrrolidone and sodium alginate.
The technical scheme of the invention is further improved as follows: the filler is selected from one or more of white kaolin, washing kaolin, organic bentonite, light calcium carbonate, calcium bicarbonate, corn starch, sodium gluconate, talcum powder and diatomite.
The technical scheme of the invention is further improved as follows: the number of live spores of the bacillus methylotrophicus 9912 is 1-100 hundred million live spores/gram.
Due to the adoption of the technical scheme, the invention has the technical progress that:
the bactericide-metalaxyl-M is mainly used for preventing and treating the root rot of crops by compounding the thalli and the bactericide, has no antagonism, but has obvious synergism, can effectively reduce the application amount of the pesticide and the cost of the pesticide, is not easy to generate resistance by mutually mixing germs, and has obvious growth promoting effect on the roots of the plants.
Detailed Description
The present invention will be described in further detail with reference to the following examples:
active ingredients of the granule comprise bacillus methylotrophicus 9912 and metalaxyl-M, and the weight ratio of the bacillus methylotrophicus 9912 to the metalaxyl-M is 20: 1-1: 20, preferably 10: 1. The granule also comprises dispersing wetting agent, adsorbent, filler and disintegrant. 0.1 to 20 percent of metalaxyl-M, 0.5 to 20 percent of dispersing wetting agent, 0.1 to 10 percent of adsorbent, 1 to 30 percent of disintegrating agent and 10 to 70 percent of filler. Wherein the number of live spores of the bacillus methylotrophicus 9912 is 1-100 hundred million live spores/g.
The dispersing and wetting agent is one or more selected from EO-PO block ether wetting dispersant, sulfonate dispersant, polyphosphate dispersant, calcium lignosulfonate, polycarboxylate dispersant, phosphate dispersant and rosin wetting dispersant.
The adsorbent is selected from one or more of white carbon black, kaolin, light calcium carbonate and bentonite.
The disintegrating agent is selected from one or more of sodium chloride, sodium sulfate, sodium carboxymethyl starch, ammonium sulfate, urea, polyvinylpyrrolidone, and sodium alginate.
The filler is selected from one or more of white kaolin, washing kaolin, organic bentonite, light calcium carbonate, calcium bicarbonate, corn starch, sodium gluconate, talcum powder and diatomite.
Example 1
Weighing 10 percent of 80 hundred million/g live spore methylotrophic bacillus mother medicine, 1 percent of metalaxyl-M, 4 percent of polycarboxylate, 5 percent of sulfonate, 5 percent of white carbon black, 20 percent of ammonium sulfate and the balance of corn starch according to the weight ratio of the methylotrophic bacillus 9912 to the metalaxyl-M of 10:1, putting the raw materials into a stirring tank, stirring for 1-2 hours to fully and uniformly mix the raw materials, crushing the mixture by air flow to obtain finished product particles with the particle size of less than 40 mu m, adding a small amount of water, kneading, and granulating by a disc granulator.
Example 2
Weighing 0.2 percent of a 30 hundred million/g live spore bacillus methylotrophicus mother drug, 0.1 percent of metalaxyl-M, 0.5 percent of EO-PO block ether wetting dispersant, 1 percent of light calcium carbonate, 9 percent of sodium carboxymethyl starch and the balance of corn starch according to the weight ratio of the bacillus methylotrophicus 9912 to the metalaxyl-M, adding the raw materials into a stirring tank, stirring for 1-2 hours to fully and uniformly mix, crushing the mixture into finished product particles with the particle size of less than 40 mu m by air flow, adding a small amount of water, kneading, and granulating by using a disc granulator.
Example 3
Weighing 1% of a 100 hundred million/g live spore bacillus methylotrophicus mother medicine, 20% of metalaxyl-M, 20% of phosphate, 10% of kaolin, 30% of ammonium sulfate and the balance of talcum powder according to the weight ratio of the bacillus methylotrophicus 9912 to the metalaxyl-M being 1:20, putting the raw materials into a stirring tank, stirring for 1-2 hours to fully and uniformly mix the raw materials, carrying out air flow crushing on the mixture to obtain finished product particles with the particle size of less than 40 mu m, adding a small amount of water, kneading, and granulating by using a disc granulator.
Comparative example 1
Weighing 10% of a methyl nutrition type bacillus mother medicine with 80 hundred million/g live spores, 4% of polycarboxylate, 5% of sulfonate, 5% of white carbon black, 20% of ammonium sulfate and the balance of corn starch, putting the raw materials into a stirring tank, stirring for 1-2 hours to fully and uniformly mix, carrying out airflow crushing on the mixture to obtain finished product particles with the particle size of less than 40 mu m, adding a small amount of water, kneading, and granulating by using a disc granulator.
Comparative example 2
Weighing 1% of metalaxyl-M, 4% of polycarboxylate, 5% of sulfonate, 5% of white carbon black, 20% of ammonium sulfate and corn starch to supplement the balance, putting the raw materials into a stirring tank to be stirred for 1-2 h to be fully and uniformly mixed, carrying out air flow crushing on the mixture to obtain finished product particles with the particle size of less than 40 mu m, adding a small amount of water to knead, and granulating by using a disc granulator.
The embodiment of the invention adopts a method combining indoor toxicity measurement and field test. Firstly, determining the Synergistic Ratio (SR) of two medicaments compounded according to a certain proportion by indoor toxicity measurement, wherein the SR is less than 0.5 for antagonism, the SR is more than or equal to 0.5 and less than or equal to 1.5 for addition, and the SR is more than 1.5 for synergism, and then carrying out field test on the basis.
Indoor toxicity determination of the bacillus methylotrophicus and metalaxyl-M compounded agent on cucumber root rot germs:
the test method comprises the following steps: after the effective inhibition concentration range of each medicament is determined by pre-test, the medicaments are respectively treated by 5 doses according to the content of effective components, and clear water is set for comparison. The toxicity of the medicament to crop germs is determined by adopting a hypha growth rate method according to 'indoor pesticide bioassay test standard bactericide'. And measuring the colony diameter by a cross method after 72h, and calculating the net growth amount and the hypha growth inhibition rate of each treatment.
The net growth (mm) is a measure of colony diameter-5
Hypha growth inhibition (%) (control group net growth amount-treatment group net growth amount)/control group net growth amount) (% 100
Diseases to be tested: strawberry root rot; and (3) experimental design: the effective inhibition concentration range of the bacillus methylotrophicus, the metalaxyl-M technical material and the mixture of the bacillus methylotrophicus and the metalaxyl-M technical material in different proportions is determined through a preparation experiment.
And (3) virulence determination results:
table 180 hundred million live spores/gram of Bacillus methylotrophicus mother drug and metalaxyl-M are compounded in different proportions to measure the toxicity of strawberry root rot bacteria:
the table shows that when the ratio of the bacillus methylotrophicus to the metalaxyl-M is 20: 1-1: 20, the synergistic ratio SR is more than 1.5, which indicates that the two bacteria are mixed in the range of 20: 1-1: 20 to show synergistic effect, wherein when the ratio of the bacillus methylotrophicus to the metalaxyl-M is 10:1, the synergistic ratio is the largest, and the synergistic effect is most obvious.
Field efficacy test for preparing granules for preventing and treating strawberry root rot
And (3) experimental design: examples 1 to 3, control 1 and control 2 were all applied at an amount of 30g/m2Simultaneously, 1 blank control group was set, and 3 replicates were set for different treatments.
The application method comprises the following steps: selecting disease-free strawberry plots, leveling the plots, uniformly mixing the soil with root rot germs, and sequentially paving the plots on the beds to be tested for seedling raising, wherein the thicknesses of the soil with diseases on the beds are required to be consistent (about 3 cm). Planting strawberry seedling in the above soil, repeating each treatment for 3 times in the test, with a cell area of 1m2According to the experimental design, the corresponding medicaments of examples 1-3, comparative example 1 and comparative example 2 are uniformly applied to the corresponding cells according to the specified dosage, and the seedbed environment is maintained in each cellThus, the method can be used for the treatment of the tumor. The incidence of root rot was counted at 30 days.
The test results are shown in the following table:
effect of different treatments on strawberry root rot incidence
Compared with a blank control, the incidence rate of the root rot is remarkably reduced by other treatments, wherein the control effect of the bacillus methylotrophicus-metalaxyl-M granules (effective component ratio is 10:1) in example 1 on the root rot of the strawberry is the best 88.73%, and the control effect of examples 2 and 3 is more than 85% and is remarkably higher than that of other two control examples.
Claims (10)
1. A bacillus methylotrophicus and metalaxyl-M compound granule is characterized in that: the active ingredients of the granule comprise Bacillus methylotrophicus 9912 and metalaxyl-M.
2. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 1, which is characterized in that: the weight ratio of the bacillus methylotrophicus 9912 to the metalaxyl-M is 20: 1-1: 20.
3. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 2, which is characterized in that: the weight ratio of the bacillus methylotrophicus 9912 to the metalaxyl-M is 10: 1.
4. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 2 or 3, which is characterized in that: the granules also comprise a dispersing wetting agent, an adsorbent, a filler and a disintegrating agent.
5. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 4, which is characterized in that: the content of the metalaxyl-M is 0.1-20%, the content of the dispersing wetting agent is 0.5-20%, the content of the adsorbent is 0.1-10%, the content of the disintegrating agent is 1-30%, and the content of the filler is 10-70%.
6. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 5, which is characterized in that: the dispersing and wetting agent is selected from one or more of EO-PO block ether wetting dispersant, sulfonate dispersant, polyphosphate dispersant, calcium lignosulfonate, polycarboxylate dispersant, phosphate dispersant and rosin wetting dispersant.
7. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 5, which is characterized in that: the adsorbent is selected from one or more of white carbon black, kaolin, light calcium carbonate and bentonite.
8. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 5, which is characterized in that: the disintegrating agent is selected from one or more of sodium chloride, sodium sulfate, sodium carboxymethyl starch, ammonium sulfate, urea, polyvinylpyrrolidone and sodium alginate.
9. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 5, which is characterized in that: the filler is selected from one or more of white kaolin, washing kaolin, organic bentonite, light calcium carbonate, calcium bicarbonate, corn starch, sodium gluconate, talcum powder and diatomite.
10. The bacillus methylotrophicus and metalaxyl-M compound granule according to claim 5, which is characterized in that: the number of live spores of the bacillus methylotrophicus 9912 is 1-100 hundred million live spores/gram.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911158333.6A CN110973162A (en) | 2019-11-22 | 2019-11-22 | Bacillus methylotrophicus and metalaxyl-M compound granule |
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| CN201911158333.6A CN110973162A (en) | 2019-11-22 | 2019-11-22 | Bacillus methylotrophicus and metalaxyl-M compound granule |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195135A (en) * | 2020-11-06 | 2021-01-08 | 青岛农业大学 | Bacillus belgii and application thereof in preventing and treating plant downy mildew disease |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222076A (en) * | 2014-06-30 | 2014-12-24 | 华北制药集团爱诺有限公司 | Bacillus methylotrophicus wettable powder and preparation method and application thereof |
| CN109486703A (en) * | 2018-10-18 | 2019-03-19 | 淮海工学院 | Methylotrophic bacillus BMF 04 and fermentation process and purposes |
-
2019
- 2019-11-22 CN CN201911158333.6A patent/CN110973162A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222076A (en) * | 2014-06-30 | 2014-12-24 | 华北制药集团爱诺有限公司 | Bacillus methylotrophicus wettable powder and preparation method and application thereof |
| CN109486703A (en) * | 2018-10-18 | 2019-03-19 | 淮海工学院 | Methylotrophic bacillus BMF 04 and fermentation process and purposes |
Non-Patent Citations (1)
| Title |
|---|
| 余本水: "《原药供应手册 （2007-2008年版）》", 31 October 2007, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195135A (en) * | 2020-11-06 | 2021-01-08 | 青岛农业大学 | Bacillus belgii and application thereof in preventing and treating plant downy mildew disease |
| CN112195135B (en) * | 2020-11-06 | 2022-02-18 | 青岛农业大学 | Bacillus belgii and application thereof in preventing and treating plant downy mildew disease |
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Application publication date: 20200410 |