CN1176878C - Composit method for preventing plant soil spread diseases - Google Patents
Composit method for preventing plant soil spread diseasesInfo
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- CN1176878C CN1176878C CNB021125589A CN02112558A CN1176878C CN 1176878 C CN1176878 C CN 1176878C CN B021125589 A CNB021125589 A CN B021125589A CN 02112558 A CN02112558 A CN 02112558A CN 1176878 C CN1176878 C CN 1176878C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention relates to a farmyard fertilizer method for preventing and curing plant soil spread diseases, which belongs to the technical field of crop disease prevention and cure. Biologic bacterium proof bacterial strain bacilli, pseudomonas, serratieae, escherichia and corynebacteria are prepared into a biologic bacterium proof agent, and the total concentration of viable bacteria is from 1*10 <9> to 1*10 <14> cfu/ml; every 100 kilograms (liters) of organic fertilizers are added with 1 to 10, 000 ml of the biologic bacterium proof medicine agent; the stacking of the organic fertilizers added with the biologic bacterium proof medicine agent is carried out for 3 to 30 days at a normal temperature, the humidity is from 40 to 100%, and farmyard fertilizers are prepared. The plant soil spread disease prevention and cure effect of the farmyard fertilizers is more than 85%, and simultaneously, the farmyard fertilizers can improve soil quality. The output of fields without plant soil spread diseases can be increased by more than three times. The present invention has low cost and simple operation, and has an important significance to the continuous utilization of farm field soil.
Description
A kind of composting process of preventing and treating plant soil-borne diseases belongs to the farm crop technical field of preventing and curing diseases, and is exclusively used in the non-environmental pollution control of field soil-borne disease.
Though the extensive morbidity that the using of chemical prevention disease that the 60 to 70's began and chemical fertilizer occurs in the intensive cropping as agriculture production and the solution of problem of fertilization play an important role, but, also brought the influence of many secondary faces, for example poisoning, soil compaction, output are successively decreased etc. year by year, in Jiangsu, a plurality of provinces such as Anhui, Shaanxi have been found that the equal poor growth of any crop in some plot, and even all seldom growths of weeds.At present each developed country has been noted that the seriousness of this problem, and careful usefulness or forbid some chemicals and fertilizer is advocated organic fertilizer and biological control.The effect of land for growing field crops biological control at present is general only in the scope of 30 ∽ 60%.In plant pest management, the most successful with insect biological and ecological methods to prevent plant disease, pests, and erosion especially Bt biological pesticide control lepidoptera pest.Disease control is backward relatively, except that K84 control fruit root knot disease is achieved success and has been applied, there are not other effective biomedicinal or method to prevent and treat disease as yet, especially cause the bacterial wilt, eqpidemic disease, blight of whole strain dead and even full field total crop failure etc., the report of Shang Weiyou success (Chen Tao chief editor, 1995, " microbial control principle and the technology of harmful organism ", Wuhan: Hubei science tech publishing house).So the method for seeking a kind of effective control plant soil-borne diseases and improvement soil property is imperative.
Compost is under in check envrionment conditions, the biological degradation of the organic composition in the refuse.This method has certain effect in the control of soil-borne disease, this all early had research at home and abroad.Nagano mountain valley in Japan, the compost of bark of dahurian larch is used for preventing and treating the red rotten sickle-like bacteria of Chinese Chinese yam by the vegetable grower, the compost of this height lignifying, high stable bark, use with 30mt/ha, can postpone the generation (Harry A is ∽ 114 J.1986.Ann.Rev.Phytopathol.24:93) of disease.Similar common compost generally continues more than one month, multiple action of microorganisms is wherein arranged, the microorganism that the different times of the microbe species that works, the mode of action of different microorganisms and compost plays a major role is all inequality, effect to different compost has tangible influence, and these influences also are difficult to control so far.The compost time is oversize or too short, all is unfavorable for the control of disease.
The objective of the invention is at disease control in the present biological control technology backward in techniquely, the present situation of prevention effect difference provides a kind of composting process of preventing and treating plant soil-borne diseases, and preventing disease theraping effect is reached more than 70%, improves soil property, increasing both production and income simultaneously.Be particularly useful for taking place for a long time soil-borne disease, perhaps soil compaction, all dysgonic field of any crop piece.
A kind of composting process of preventing and treating plant soil-borne diseases of the present invention, its technical scheme is:
1) biocontrol bacteria bacterial strain genus bacillus (Bacillus sp.), pseudomonas (Pseudomonassp.), Serratia (Serratia sp.), Escherichia (Escherichia sp.), excellent bacillus (Corynebacterium sp.) are made single biocontrol fungicide or two kinds, three kinds bacteriums are hybridly prepared into biocontrol fungicide, and the viable bacteria total concn is 1 * 10 in the finished product
9∽ 1 * 10
14Cfu/ml;
2) add 1 ∽ 10 in per 100 kilograms solid organic fertilizer or per 100 liters liquid organic fertilizer, the ratio of the above-mentioned biocontrol bacteria medicament of 000ml is made composting material after stirring;
3) with the composting material made when 15 ℃ of temperature 5 ∽, the compost time is 25 ∽ 30 days; During 25 ℃ of temperature 15 ∽, the compost time is 13 ∽ 15 days; During 30 ℃ of temperature 25 ∽, the compost time is 5 ∽ 8 days; Temperature is more than 30 ℃ the time, and the compost time is 3 ∽ 4 days, and humidity 40 ∽ 100% make compost.
Used biocontrol strain is genus bacillus B130, FH17, BB11 in the above-mentioned compost method, pseudomonas J3, Serratia J2, Escherichia BT4, excellent bacillus BT6.The ratio that can make single microbial inoculum respectively or measure 1.0: 1.0 ∽ 0.3 of 0.3 ∽ in bacterium is J2 and J3, BT4 and BT6, and FH17 and BB11, BT4 and J2, BT6 and J3, J3 and BB11 are hybridly prepared into the biocontrol bacteria medicament in twos; Perhaps with J2, J3 and BB11, three kinds of bacterium of BT4, J3 and BB11 are used than being hybridly prepared into the biocontrol bacteria medicament with the bacterium amount of 0.3: 1.0 ∽ 0.3 of 1.0: 1.0 ∽ of 0.3 ∽.
The used microbial inoculum making method of the present invention is conventional method for cultivation of bacteria.Used biocontrol strain genus bacillus B130 (Bacillus sp.), FH17 (Bacillus sp.), BB11 (Bacillus sp.) and Serratia J2 (Serratia sp.), pseudomonas J3 (Pseudomonas sp.), Escherichia BT4 (Escherichia sp.), excellent bacillus BT6 (Corynebacterium sp.) are known public bacterial strain.Wherein B130 see document 1 (preliminary study of the anti-blue or green withered biocontrol microorganisms antagonist character of .1994. such as Guo Jianhua. the journal .18 of Agricultural University Of Nanjing (2): 59 ∽ 62.); All the other bacterial strains all see document 2 (screening of .2001. pepper ralstonia solanacearum antagonistic strains such as Guo Jianhua and the mensuration of field efficacy. Chinese biological control .17 (3): 101 ∽ 106).
Above-mentioned biocontrol bacteria can come from the subordinate: the Gram-negative or the gram-positive eubacterium of everyone and animals and plants non-virulent such as Rhodopseudomonas, pantoea agglomerans genus, serratia, streptomyces, bacillus and Corynebacterium.This compost method can be used for preventing and treating vegetative bacteria soil-borne disease bacterial wilt, ring rot, soft rot, Peptic Ulcers, basal stem rot, wilt disease, seedling blight, yellowtop, and fungoid soil-borne disease eqpidemic disease, blight, southern blight, the control of late blight, verticillium, sclerotium disease, root rot, damping-off.
The present invention also can use other multi-purpose agricultural active bacteria formulation product of having developed both at home and abroad, comprises preventing and treating bacteria agents disease, degrading pesticide residues or other objectionable constituent.In exsiccant fertilizers such as soyabean cake or animal dunghill preferably with behind 500 times of the medicament dilute with waters again row admix, keep certain humidity in the compost, prevent natural rainwash simultaneously.If in the higher booth of humiture, can select the less corner compost of illumination.Above-mentioned fertilizer can be soyabean cake, corn-dodger, chicken manure, pig manure, cow dung, wheat bran, rice chaff, wood chip, forage etc. or above-mentioned several or multinomial mixture (it is just effective that wheat bran, rice chaff, wood chip must mix use with other fertilizer).Preferably biological and ecological methods to prevent plant disease, pests, and erosion medicament and fertilizer are stirred, utilize natural hot and humid condition, perhaps under the condition of artificial heat and moisture preserving, carry out, and in time mix (ploughing) and go into to need in the soil of diseases prevention or improvement.
The present invention compared with prior art, its advantage and positively effect show:
The present invention quantitatively adds the biocontrol microorganisms medicament in compost, controlled the fermentation direction of microorganism in the compost effectively, make the external microorganism of introducing that a large amount of adaptability breedings is arranged in compost simultaneously, to it in the adaptation of crop root with grow surely and play an important role, thereby more help the performance of biological and ecological methods to prevent plant disease, pests, and erosion medicament effect.Temperature according under the different natural condition has also designed the best compost time respectively.
Test shows, this method single bacteria preparation of banking up has effect more than 65% to the control plant soil-borne diseases; The microbial inoculum that two or more bacterial strains of banking up fit in has effect more than 85% to the control plant soil-borne diseases.In the peasant of every family, promote the use of with suiting measures to local conditions, simple to operate, with low cost, a season of growth only need use once, ripe compost can also be improved soil property and soil microorganisms composition simultaneously, improves the upgrowth situation of field crops significantly, promotes increasing both production and income.Can carry out big area to staple crops such as paddy rice, wheats and use, be particularly useful for the plantation of vegetables such as capsicum, tomato, Chinese cabbage in the booth, can prevent and treat multiple soil-borne diseases such as crop bacterial wilt, eqpidemic disease, more than anosis field can increase income 3 one-tenth.The more important thing is,, the Sustainable development of agriculture production is had great significance the sustainability utilization of agricultural land soil.
Embodiment 1: 1 mu of tomato ground of Nanjing man of Xixia District herds of horses peasant household, planted tomato continuously 3 years.This peasant family custom before capsicum is transplanted in every year applies in the plastic shed soil as fertilizer with animal muck and forage, straw mixing.Sickness rate was about 45% because the infecting of bacterial wilt in 1999.Spring in 1999, this seminar chose the non-environmental pollution control test that composting process field soil-borne disease is carried out in this plot.Its process is as follows:
Institute's with medicament is the biocontrol bacteria medicament that genus bacillus BB11 bacterial strain is made, and concentration is 1 * 10
10Cfu/ml adds with the ratio that adds 1000ml in per 100 kilograms of fertilizers, is stacked at the field after stirring, and this moment, temperature was 20 ℃ of 15 ∽.Humidity maintains 60 ∽ 80% in the compost.After 15 days, the organic fungi-manure that heap is good is sprinkled into the field, in cultivated the burying.6 sub-districts of repetition are established in this test, every sub-district 400 strain tomatoes.Tomato seedling normally is transplanted in the canopy.30 days, 60 days and gather before statistics morbidity strain number respectively, statistics output (table 1).
Table 1. herds of horses tomato biological and ecological methods to prevent plant disease, pests, and erosion test-results
| Processing scheme | Sickness rate (%) | Protection effect (%) | Output (kilogram/mu ground) | |
| Control group | Directly apply fertilizer | 47 | - | 1850 |
| Test group | BB11 microbial inoculum+fertilizer → make compost | 10 | 78.7 | 2750 |
Embodiment 2: the capsicum booth of the bamboo frame structure of Huai Chu Zhou district yellow sign indicating number desirable village, township peasant Zhang Yanjia, totally 1 mu of ground.The red Bian green pepper of plantation is 3 years continuously, and this peasant family is accustomed to applying in the plastic shed soil as fertilizer with soyabean cake every year before capsicum is transplanted.Sickness rate was 95% because the mixed infection of bacterial wilt and eqpidemic disease in 1999, and whole booth is close to total crop failure (receiving about 300 kilograms of capsicum altogether), had nearly 1/5 field piece to take out in the canopy successively to sow behind the capsicum green vegetables, radish all to fail germination.Autumn in 2000, this seminar chose the non-environmental pollution control test that composting process field soil-borne disease is carried out in this plot.Its process is as follows:
Institute's with medicament is the biocontrol bacteria medicament that J2 and J3 bacterial strain are made with balanced mix, and concentration is 1 * 10
9Cfu/ml, to add 10 in per 100 kilograms of soyabean cakes, the ratio of 000ml adds, and is stacked in the booth after stirring, and this moment, canopy temperature was 30 ℃ of 25 ∽.Humidity maintains 70 ∽ 90% in the compost.Three kinds of contrasts are established in this test, and every kind of contrast repeats three sub-districts, every sub-district 120 strain capsicums.Table 1 is pressed in the design of three groups of contrasts.After 7 days, the organic fungi-manure that heap is good is sprinkled into the field, in cultivated the burying.The capsicum seedling normally is transplanted in the canopy, 7 minutes Di transplants capsicum 2560 strains altogether.30 days, 60 days and gather before statistics morbidity strain number respectively, morbidity strain number is 11 strains in 7 fens ground, and does not increase all the time in process of growth, sickness rate is less than 1%.
Observe the chili growth situation, find that the capsicum growing way is very vigorous in the monoblock booth, the original long plot of green vegetables, radish, the growth of capsicum is healthy and strong equally.Illustrate that the composting process that the present invention prevents and treats plant soil-borne diseases has improving effect to soil, because available fertilizer ratio increases in the sophisticated compost on the one hand, on the other hand owing to introduce the bulk fermentation of biocontrol bacteria, improved the structure of original microorganism in the soil after making compost apply the field, made it reach a new eubiosis towards helping the plant growth aspect.The output of disposable statistics red pepper, 7 minutes receive about 1000 kilograms of capsicum (seeing Table 2).
Table 2. capsicum biological and ecological methods to prevent plant disease, pests, and erosion test design and result
| Processing scheme | Sickness rate (%) | Protection effect (%) | Output (kilogram/mu ground) | |
| Control group a | Directly apply soyabean cake | 98 | - | 10 |
| Control group b | Only use soyabean cake compost, no biocontrol microorganisms medicament | 80 | 18.4 | 350 |
| Control group c | Soyabean cake directly applies fertilizer, and biocontrol fungicide pouring method is used | 70 | 28.6 | 460 |
| Test group | Biocontrol fungicide+soyabean cake → make compost | 0.1 | 100 | 1429 |
By present embodiment as seen, the pouring of simple compost and simple biocontrol microorganisms all can alleviate the generation (protection effect 18.4% and 28.6%) of disease to a certain extent, improves output.Both compounding applications then can make protection effect and output be multiplied, and soil-borne disease does not almost take place at this season.
Embodiment 3: the capsicum booth of the cement shelf structure of Huai Chu Zhou district Liu surname peasant family, totally 1 mu of ground.Plantation red Bian green pepper 2 years continuously will provide all before annual capsicum is transplanted that about 100L ight soil applies in the plastic shed soil in the septic tank for oneself.Last year in 2000, sickness rate was 100% because of the mixed infection of multiple soil-borne disease, and whole booth is received about 50 kilograms of capsicum altogether.In spring calendar year 2001, this seminar chooses the non-environmental pollution control test that composting process field soil-borne disease is carried out in this plot.Its process is as follows:
Institute's with medicament is that FH17 and BB11 bacterial strain are measured than the biocontrol bacteria medicament that is mixed and made into 0.3: 1 bacterium, and concentration is 1 * 10
11Cfu/ml pours 100ml biological and ecological methods to prevent plant disease, pests, and erosion medicament in the septic tank that fills about 100L ight soil into, stirs, and covers plastic film, and this moment, temperature was about 15 ℃ of 10 ∽, humidity 100%.After 25 days, organic fungi-manure that heap is good is converted water and is watered and bury.The capsicum seedling normally is transplanted in the canopy, transplants 2308 strains altogether.30 days, 60 days and gather before the time add up sickness rate respectively, sickness rate is respectively 0.48%, 0.26%, 0.22%.The capsicum growing way is very vigorous in the monoblock booth.The output of statistics red pepper, this mu are experimental field in this season of growth 14,00 kilograms in common property capsicum.
Embodiment 4: the tomato canopy of the bamboo frame structure of Nanjing Xixia District herds of horses township Yuan surname peasant family, totally 4 fens ground.Planted tomato continuously 3 years.Sickness rate was 85% because the mixed infection of tomato wilt and bacterial wilt in 1999, receive approximately 280 kilograms of tomatoes.Spring in 2000, this seminar chose the non-environmental pollution control test that composting process field soil-borne disease is carried out in this plot.Its process is as follows:
Institute's with medicament is that J2, J3 and three kinds of bacterium of BB11 are measured than the biocontrol bacteria medicament that is mixed and made into 1.0: 0.3: 1.0 bacterium, and concentration is 1 * 10
13Cfu/ml, the ratio adding with 5ml biocontrol bacteria medicament in the mixing fertilizer of per 100 kilograms of various animal excrement and plant ash, wheat bran etc. is stacked in the booth, and this moment, temperature of shed was 20 ℃ of 15 ∽.Humidity maintains 50 ∽ 100% in the compost.After 15 days, the organic fungi-manure that heap is good is sprinkled into the field, during hoe buries.Tomato seedling normally is transplanted in the canopy.Statistics morbidity strain number has only 1 strain morbidity dead, and does not increase all the time in process of growth before gathering in 4 fens ground, and sickness rate is less than 0.1%.Observe the tomato growth situation, find that the tomato growing way is very vigorous in the monoblock booth, significantly better than the tomato of former years and fellow villagers family.The output of statistics tomato is received to such an extent that tomato is total to about 1120 kilograms (4 fens ground).Identical fertilizer has been executed in the control group plot, does not add biocontrol microorganisms, does not also have compost, and sickness rate is 90%, 4 minute Di only to receive about 200 kilograms of tomato.
Embodiment 5: the cucumber booth of 2 steel framed structures of Dongying village, the Fugou County Chai Gang of Zhoukou Prefecture, Henan Province township will man of peasant Lee party, 9 fens ground of every canopy.Planted cucumber continuously 3 years, this peasant family is accustomed to applying in the plastic shed soil as fertilizer with the animal muck every year before cucumber is transplanted.Sickness rate was 99% because the infecting of wilt in 1999, and whole booth is close to total crop failure (receiving about 100 kilograms of cucumber altogether).Autumn in 2000, this seminar chose the non-environmental pollution control test that composting process field soil-borne disease is carried out in this plot.Its process is as follows:
Institute's with medicament is that J2, J3 and three kinds of bacterium of BB11 are measured than the biocontrol bacteria medicament that is mixed and made into 1.0: 1.0: 1.0 bacterium, and concentration is 1 * 10
12Cfu/ml adds with the ratio that adds 50ml in per 100 kilograms of muck, stirs after adding water, is stacked in the booth, and this moment, canopy temperature was 25 ℃ of 20 ∽.Humidity maintains 40 ∽ 90% in the compost.After 10 days, the organic fungi-manure that heap is good is sprinkled into the field, in cultivated the burying.Only use muck routinely in the contrast canopy, do not add biocontrol fungicide.Cucumber seedling normally is transplanted in the canopy, 9 minutes Di transplants cucumber 4500 strains altogether.Statistics morbidity strain number before gathering, morbidity strain number is 38 strains, sickness rate is less than 1%.9 minutes receive about 1500 kilograms of cucumber, average per minute ground gathers in the crops 167 kilograms.Add up the sickness rate and the harvest of each control group, calculate protection effect (seeing Table 3).
Table 3. cucumber biological and ecological methods to prevent plant disease, pests, and erosion test design and result
| Processing scheme | Sickness rate (%) | Protection effect (%) | Output (kilogram/9 fens ground) | |
| Control group | The animal muck is directly used | 98 | - | 100 |
| Test group | Biocontrol fungicide+animal muck → make compost | 0.8 | 99 | 1500 |
By present embodiment as seen, the composting process that the present invention prevents and treats plant soil-borne diseases can obtain 99% soil-borne disease of cucumber prevention effect, makes output increase by 14 times.
Embodiment 6: Inner Mongolia Autonomous Region Bayannaoer League has 200,000 mu of melonries, majority is planted cucumber more than 3 years continuously, from 1999, owing to be subjected to the mixed infection that soil passes bacteroidal fruit blotch, yellowtop and fungoid blight, sickness rate reaches more than 80%, and production loss is serious.It is that the non-environmental pollution control test of composting process field soil-borne disease is carried out in 100% plot that this seminar in 2000 chooses sickness rate.Its process is as follows:
Institute's with medicament is that BT4, J3 and three kinds of bacterium of BB11 are measured than the biocontrol bacteria medicament that is mixed and made into 0.5: 1.0: 0.8 bacterium, and concentration is 1 * 10
14Cfu/ml adds with the ratio that adds 1ml in per 100 kilograms of muck, stirs after adding water, is stacked at the field, and this moment, temperature was 15 ℃ of 10 ∽.Humidity maintains 40 ∽ 80% in the compost.After 25 days, the organic fungi-manure that heap is good is sprinkled into the field, in cultivated the burying.Only use muck routinely in the piece of contrast field, do not add biocontrol fungicide.The muskmelon seedling normally is transplanted in the canopy.The statistics sickness rate is 1% before gathering.Observe the muskmelon upgrowth situation, find that interior en bloc muskmelon growing way is vigorous.The output of disposable statistics muskmelon, 1 mu of ground receive about 2100 kilograms of muskmelon.Add up the sickness rate and the harvest of each control group, calculate protection effect (seeing Table 4).
Table 4. muskmelon biological and ecological methods to prevent plant disease, pests, and erosion test design and result
| Processing scheme | Sickness rate (%) | Protection effect (%) | Output (kilogram/mu ground) | |
| Control group | The animal muck is directly used | 80 | - | 200 |
| Test group | Biocontrol fungicide+animal muck → make compost | 1.0 | 98.8 | 2100 |
By present embodiment as seen, the composting process that the present invention prevents and treats plant soil-borne diseases can obtain gratifying muskmelon soil-borne disease prevention effect 98.8%, and output is multiplied.
Embodiment 7: the cucumber booth of 2 steel framed structures in Fugou County, Zhoukou Prefecture, Henan Province, 1 mu of ground of every canopy.Planted cucumber continuously 3 years, this peasant family is accustomed to applying in the plastic shed soil as fertilizer with the animal muck every year before cucumber is transplanted.1999 and 2000 because multiple factors such as chemical fertilizer and chemical pesticide cause Soil structure to be damaged, though soil-borne disease does not take place, but but poor growth of crop wherein, 2000 more so, has nearly 1/3 field piece to take out in the canopy successively to sow green vegetables, radish behind the cucumber all to fail to germinate.Calendar year 2001, this seminar chose the non-environmental pollution control test that composting process field soil-borne disease is carried out in this plot.Its process is as follows:
Institute's with medicament is that J2, J3 and three kinds of bacterium of BB11 are measured than the biocontrol bacteria medicament that is mixed and made into 0.5: 1.0: 1.5 bacterium, and concentration is 1 * 10
13Cfu/ml adds with the ratio that adds 10ml in per 100 kilograms of muck, stirs after adding water, is stacked in the booth, and this moment, canopy temperature was 25 ℃ of 15 ∽.Humidity maintains 40 ∽ 90% in the compost.After 10 days, will pile good organic fungi-manure immediately and be sprinkled into the field, in cultivated the burying.Cucumber seedling normally is transplanted in the canopy.Observe the cucumber growth situation, find that the cucumber growing way is vigorous in the monoblock booth.The output of disposable statistics cucumber, 1 mu of ground receive about 1700 kilograms of cucumber, volume increase 100%.
With above same method, agent of biocontrol fungicide list or mixed preparation compost are used for other vegetables or cash crop, the biological and ecological methods to prevent plant disease, pests, and erosion test effect all can reach more than 85% (table 5).
Use the effect of compost in table 5. various crop and the floral crops diseases control
| Crop and disease | Biocontrol strain | Bacterium amount ratio | Viable bacteria total concn (cfu/ml) | Composting material | Medicament: compost (ml/100Kg.L) | Temperature | Humidity (%) | The compost fate | Protection effect (%) | Volume increase (%) |
| Bacterial wilt of tomato | BB11 | 1×10 9 | The animal muck | 8000 | 25∽30℃ | 100 | 7 | 80 | 65 | |
| Soft rot of Chinese cabbage | J2 | 1×10 10 | The animal muck | 2000 | 5∽15℃ | 100 | 30 | 85 | 78.5 | |
| Pepper ralstonia solanacearum | J3 | 1×10 11 | The animal muck | 600 | 15∽20℃ | 80 | 8 | 72.4 | 82.8 | |
| Pepper ralstonia solanacearum | BT4 | 1×10 12 | The animal muck | 300 | 15∽20℃ | 80 | 8 | 71.3 | 33.3 | |
| Pepper ralstonia solanacearum | FH17 | 5×10 12 | The animal muck | 100 | 15∽20℃ | 80 | 8 | 66.8 | 46.5 | |
| Bacterial wilt of ginger | B130 | 1×10 13 | The animal muck | 100 | 20∽25℃ | 90 | 14 | 80 | 76 | |
| Pepper ralstonia solanacearum and eqpidemic disease | J2，J3 | 0.4∶1 | 6×10 13 | Soyabean cake | 10 | 25∽30℃ | 60 | 7 | 90 | 120 |
| Pepper ralstonia solanacearum and eqpidemic disease | BT4，BT6 | 1∶0.6 | 1×10 14 | Soyabean cake | 1 | 25∽30℃ | 60 | 7 | 90 | 156 |
| Pepper ralstonia solanacearum and eqpidemic disease | FH17，BB11 | 0.9∶1 | 3×10 11 | Soyabean cake | 270 | 25∽30℃ | 60 | 7 | 95 | 265 |
| The chrysanthemum blight | BT4，J2 | 1∶0.5 | 1×10 12 | Leaf+green grass+muck | 300 | 25∽30℃ | 70 | 8 | 95 | 260 |
| Pepper ralstonia solanacearum and eqpidemic disease | J3，BB11， | 0.3∶1 | 1×10 9 | Leaf+green grass+muck | 10,000 | 28∽35℃ | 70 | 4 | 95 | 65 |
| The tree peony wilt disease | BT6，J3 | 1∶0.4 | 1.5×10 9 | Leaf+green grass+muck | 5000 | 29∽35℃ | 70 | 4 | 96 | 365 |
| Pepper ralstonia solanacearum and eqpidemic disease | J2，J3，BB11 | 1∶0.4∶0.7 | 2.1×10 10 | Leaf+green grass+muck | 1000 | 29∽35℃ | 70 | 4 | 100 | 300 |
| Cucumber fusarium axysporum | J2，J3，BB11 | 0.5∶0.5∶1 | 4×10 12 | Domestic refuse+animal muck | 70 | 29∽35℃ | 40 | 4 | 100 | 80 |
| Sclerotinia rot of colza | BT4，J3，BB11 | 0.5∶0.8∶1 | 9×10 13 | Domestic refuse+animal muck | 10 | 10∽15℃ | 40 | 4 | 100 | 85 |
Claims (2)
1, a kind of composting process of preventing and treating plant soil-borne diseases is characterized in that:
1) with biocontrol bacteria genus bacillus (Bacillus sp.) bacterial strain B130, FH17, BB11, pseudomonas (Pseudomonas sp.) bacterial strain J3, Serratia (Serratia sp.) bacterial strain J2, Escherichia (Escherichia sp.) bacterial strain BT4, excellent bacillus (Corynebacterium sp.) bacterial strain BT6 makes single biocontrol fungicide or two kinds, three kinds bacteriums are hybridly prepared into biocontrol fungicide, the viable bacteria total concn is 1 * 10 in the finished product
9∽ 1 * 10
14Cfu/ml;
2) add 1 ∽ 10 in per 100 kilograms solid organic fertilizer or per 100 liters liquid organic fertilizer, the ratio of the above-mentioned biocontrol bacteria medicament of 000ml is made composting material after stirring;
3) with the composting material made when 15 ℃ of temperature 5 ∽, the compost time is 25 ∽ 30 days; During 25 ℃ of temperature 15 ∽, the compost time is 13 ∽ 15 days; During 30 ℃ of temperature 25 ∽, the compost time is 5 ∽ 8 days; Temperature is more than 30 ℃ the time, and the compost time is 3 ∽ 4 days, and humidity 40 ∽ 100% make compost.
2, a kind of composting process of preventing and treating plant soil-borne diseases according to claim 1, it is characterized in that: bacterium biological and ecological methods to prevent plant disease, pests, and erosion medicament refers to biocontrol strain genus bacillus B130, FH17, BB11, pseudomonas J3, Serratia J2, Escherichia BT4, rod bacillus BT6 makes single microbial inoculum respectively or measures the ratio of 1.0: 1.0 ∽ 0.3 of 0.3 ∽ with J2 and J3 in bacterium, BT4 and BT6, FH17 and BB11, BT4 and J2, BT6 and J3, J3 and BB11 are hybridly prepared into the biocontrol bacteria medicament in twos; Perhaps with J2, J3 and BB11, three kinds of bacterium of BT4, J3 and BB11 are measured than being hybridly prepared into the biocontrol bacteria medicament with the bacterium of 0.3: 1.0 ∽ 0.3 of 1.0: 1.0 ∽ of 0.3 ∽.
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| CNB021125589A CN1176878C (en) | 2002-01-18 | 2002-01-18 | Composit method for preventing plant soil spread diseases |
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| CNB021125589A CN1176878C (en) | 2002-01-18 | 2002-01-18 | Composit method for preventing plant soil spread diseases |
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| CN1176878C true CN1176878C (en) | 2004-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021125589A Expired - Fee Related CN1176878C (en) | 2002-01-18 | 2002-01-18 | Composit method for preventing plant soil spread diseases |
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| CN (1) | CN1176878C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100401898C (en) * | 2004-05-13 | 2008-07-16 | 南京农业大学 | Composite fungus agent for preventing rot knot nematode disease of vegetable in greenhouse |
| CN100401897C (en) * | 2004-05-13 | 2008-07-16 | 南京农业大学 | Composite fungus agent for preventing blight of vegetable in greenhouse |
| CN101595892B (en) * | 2009-05-27 | 2011-08-17 | 南京农业大学 | PS mixture of compound biocontrol fungicide for preventing soil-borne diseases of vegetables |
| CN102428807A (en) * | 2011-09-13 | 2012-05-02 | 上海博琛生物科技有限公司 | Periodic fermentation of agricultural organic waste and application thereof in facility cultivation |
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2002
- 2002-01-18 CN CNB021125589A patent/CN1176878C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1358693A (en) | 2002-07-17 |
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