CN117264813A - Streptomyces nojirimensis strain and application of fermentation liquor thereof in prevention and control of corn diseases - Google Patents
Streptomyces nojirimensis strain and application of fermentation liquor thereof in prevention and control of corn diseases Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
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Abstract
The invention relates to streptomyces nojirimensis and application of fermentation liquor thereof in preventing and controlling corn diseases, and belongs to the technical field of crop disease prevention and control. Biocontrol strain 9-13, named Streptomyces nojirimensis, was deposited in China general microbiological culture Collection center (CGMCC) at 28/6/2021 with the deposit number of CGMCC No.22798. The invention has the advantages that: the Streptomyces nojirimensis strain 9-13 has antagonism on pathogenic bacteria including corn megalopsis, corn small spot bacteria, corn rhizoctonia solani and corn rhizoctonia cerealis which cause various diseases of corn, the fermentation liquor can obviously inhibit the hypha growth of the corn megalopsis, corn small spot bacteria, corn rhizoctonia solani and corn rhizoctonia cerealis and the spore germination of the corn megalopsis and corn small spot bacteria, and the fermentation liquor has excellent control effect on the corn megalopsis, corn small spot disease and corn sheath blight.
Description
Technical Field
The invention relates to the technical field of crop disease control, in particular to a Streptomyces nojirimensis strain and application of fermentation liquor thereof in controlling corn diseases.
Background
Corn is a main grain and feed crop and an important industrial raw material in China, and plays an important role in national economy and agricultural production in China. In recent years, corn production is seriously damaged by diseases due to outbreaks of various disease epidemic minispecies and changes of pathogenicity of the disease epidemic minispecies, so that the corn yield is greatly lost, and the quality is bad. The number of diseases occurring on corns in China is up to 30, and the yield loss of the king rice caused by various diseases is about more than 15% of the total yield each year. The corn leaf spot is leaf disease, and the disease is generated in large areas in the northeast China, the north China, the northwest China, the southwest mountain area and the like, especially in 2012-2014, and the disease incidence area in the whole country reaches 600 ten thousand hm 2 The yield is reduced by more than 45% per year, and the loss of corn is as much as 8 hundred million kilograms per year. The corn small spot disease is also called corn stripe spot disease, is one of important diseases of corn production areas in China, and commonly and seriously occurs in warm and humid areas of yellow river and Yangtze river basin, and is often simultaneously or mixed with the large spot disease. Maize sheath blight is a worldwide disease that commonly occurs in maize producing areas and is a major hazard. In recent 10 years, along with large-area popularization of compact corn varieties, the corn planting density is gradually increased, and the increase of the nitrogen fertilizer application amount and the popularization of straw returning technology are causedThe overwintering bacteria source is accumulated year by year, so that the occurrence of the sheath blight of corn always has a aggravated trend, the disease rate is 22% -71% generally, and individual plots or varieties even reach 100%, so that the method becomes a chronic disease which restricts the high and stable yield of corn.
Because of the lack of high-resistance corn varieties, the prevention and treatment of corn large and small spot diseases and banded sclerotial blight mainly depend on chemical agents for prevention and treatment. However, with the massive use of chemical pesticides, the problem of 3R is endangered, and the quality safety of agricultural products and the agricultural ecological environment in China are seriously threatened. Therefore, there is a need to accelerate the traditional way of changing the excessive dependence of crop disease management on chemical pesticides, and there is a need to screen biological source bactericides with novel sterilization mechanisms. The colonization of the biocontrol bacteria on plant rhizosphere soil and body surface is greatly influenced by external environmental factors, and the development and the utilization research of secondary metabolites of the biocontrol bacteria have greater utilization value in the aspect of controlling plant diseases.
Ding Ting and the like, a strain of clostridium (Thielavia sp.) fungus DZGS08 is screened from eucommia ulmoides bodies, and the inhibition rate of 100mg/L fermentation liquor crude extract thereof to the sheath blight germ Rhizoctonia solani reaches 50.44%; the Yongbin and the like separate and purify endophytic bacillus subtilis Iun with strong antibacterial activity from Paris polyphylla tubers, and purify an antibacterial protein UD35 from fermentation supernatant thereof, wherein the protein has strong inhibition effect on various germs such as Rhizoctonia solani. The Philips longus is screened to obtain Pseudomonas fluorescens CB113, and the fermentation liquor has stronger inhibition activity on the corn big spot germ. The fermentation metabolite of the armillaria mellea has higher inhibition effect on the alternaria alternate. When the concentration of the metabolic solution is 10%, the inhibition effect on the two pathogenic bacteria is optimal, wherein the inhibition rate on the corn big spot bacteria is 85.56% and the inhibition rate on the corn small spot bacteria is 81.67% respectively.
The bacterial strain with wide bacteriostasis spectrum and stronger biological activity is screened, and the disease prevention mechanism of the bacterial strain is deeply researched, so that a solid theoretical basis is laid for the biological prevention and treatment of future diseases, and along with the continuous progress of scientific technology, the biological prevention and treatment of the diseases is believed to have wide development prospect and application value.
Disclosure of Invention
The invention aims at reducing the yield and quality of corn aiming at the occurrence of corn northern leaf blight, corn small leaf blight and corn sheath blight, influencing the healthy development of corn industry, and providing the application of the strain 9-13 of the streptomyces nojirimensis (Streptomyces nojiriensis) in preventing and controlling corn diseases.
The invention further aims to provide application of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth in prevention and treatment of corn diseases.
The aim of the invention is realized by the following technical scheme:
the application of the strain 9-13 of the streptomyces nojirimis (Streptomyces nojiriensis) in preventing and controlling corn diseases, wherein the strain 9-13 of the streptomyces nojirimis (Streptomyces nojiriensis) is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No.22798 and the preservation date of 2021, 6 and 28 days.
The strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) has strong antagonism on pathogenic bacteria of corn disease, namely, corn big spot pathogen (Setosphaeria turcica), corn small spot pathogen (Cochliobolus heterostrophus), corn Rhizoctonia solani (Rhizoctonia solani) and corn Rhizoctonia zeae (Rhizoctonia zeae).
The application of the fermentation liquor of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 in preventing and controlling corn diseases.
The preparation method of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth comprises the following steps: inoculating Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 into ISP2 liquid culture medium, fermenting at 26-28deg.C for 7-8d, and sterilizing with microporous membrane to obtain fermentation broth.
The application of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth in inhibiting the hypha growth of Rhizoctonia solani (Setosphaeria turcica), rhizoctonia cerealis (Cochliobolus heterostrophus), rhizoctonia solani (Rhizoctonia solani) and Rhizoctonia zeae.
The application of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth in inhibiting spore germination of Rhizoctonia solani (Setosphaeria turcica) and Rhizoctonia cerealis (Cochliobolus heterostrophus).
Compared with the prior art, the invention has the advantages that: the strain 9-13 of the streptomyces nojirimi (Streptomyces nojiriensis) has antagonism on the alternaria corn, the rhizoctonia solani and the rhizoctonia zeae, the fermentation liquor can obviously inhibit the hypha growth of the alternaria corn, the rhizoctonia solani and the rhizoctonia zeae and the spore germination of the alternaria corn and the rhizoctonia zeae, and the prepared fermentation liquor has excellent control effect on the alternaria corn, the rhizoctonia cerealis and the sheath blight corn.
In addition, the strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) is obtained from soil, is compatible with the soil environment in a harmonious manner, and has good application prospect.
Drawings
FIG. 1 shows the culture characteristics of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on medium of Gao's first.
FIG. 2 shows the inhibitory effects of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on Rhizoctonia solani (Setosphaeria turcica), rhizoctonia cerealis (Cochliobolus heterostrophus), rhizoctonia zeae (Rhizoctonia solani) and Rhizoctonia zeae) under the opposing culture conditions. And (3) injection: a is corn big spot germ, B is corn small spot germ, C is corn rhizoctonia solani, D is corn rhizoctonia cerealis, and CK is a control group.
FIG. 3 shows the inhibitory effect of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the growth of mycelium of Rhizoctonia cerealis (Setosphaeria turcica), rhizoctonia cerealis (Cochliobolus heterostrophus), rhizoctonia zeae (Rhizoctonia solani) and Rhizoctonia zeae). And (3) injection: a is corn big spot germ, B is corn small spot germ, C is corn rhizoctonia solani, D is corn rhizoctonia cerealis, and CK is a control group.
FIG. 4 shows the inhibitory effect of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on spore germination of Rhizoctonia solani (Setosphaeria turcica) and Rhizoctonia cerealis (Cochliobolus heterostrophus). And (3) injection: a is spore germination of the corn big spot germ after 9-13 fermentation liquor treatment, B is spore germination of the corn small spot germ after 9-13 fermentation liquor treatment, and CK is a control group.
Detailed Description
The present invention is described in detail below with reference to the drawings and examples of the specification:
embodiment one: isolation and identification of Streptomyces nojirimoniae (Streptomyces nojiriensis) strain 9-13.
1. Soil sample collection
3 parts of soil samples are collected from Jiangxi Jinggang mountains, soil on the surface is removed, soil samples in the depth of 5-20cm are collected, and the soil samples are marked and then brought back to a laboratory for natural air drying.
2. Isolation of actinomycetes
The separation was performed by plate dilution. Grinding air-dried soil sample with mortar, suspending 1g of sample in 9mL of sterile water, shaking at 40deg.C and 180rpm for 30min, standing for 5min, sequentially diluting 10 times, and respectively preparing into 10 -2 、10 -3 、10 -4 Respectively sucking 0.1mL of each suspension with different concentrations, adding the suspension into a modified HVA culture medium (adding potassium dichromate with the final concentration of 100-200 ppm) plate, uniformly coating, then inverting the solution, culturing at 28 ℃, picking different single bacterial colonies after 5-7 days, streaking and purifying, and preserving the purified bacterial strains in a refrigerator at-80 ℃ by adopting a glycerol method.
3. Identification of Streptomyces nojirimensis (Streptomyces nojiriensis) Strain 9-13
(1) Morphological feature observations
Streptomyces nojirimoniae (Streptomyces nojiriensis) strain 9-13 grew well on most media and did not produce soluble pigments (Table 1). Under an optical microscope, the strain 9-13 has straight spore filaments, flexible filaments, hooks, loose filaments, tight spirals, oval spores and columns.
Table 1 culture characteristics of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13
(2) Physiological and biochemical characteristics
The characteristics of Streptomyces nojirimonia (Streptomyces nojiriensis) such as 9-13 nitrate reduction and carbon and nitrogen source utilization were measured by the method described in Streptomyces appraisal manual, and the results are shown in Table 2.
Table 2 physiological and biochemical characteristics of Streptomyces nojirimensis (Streptomyces nojiriensis) Strain 9-13
(3) Sequence analysis
After bacterial genome extraction kit extracts bacterial 9-13 genome DNA, respectively amplifying 16S rRNA and rpoB genes, obtaining sequences with the total length of 1409bp and 859bp, submitting the obtained sequences to GenBank database for BLAST comparison analysis, and obtaining bacterial strain 9-13 which is Streptomyces nojirimensis.
Embodiment two: antagonism of the strain 9-13 of Streptomyces nojirimensis (Streptomyces nojiriensis) against 4 pathogenic bacteria of different corn diseases was determined.
Antagonism of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 against Rhizoctonia solani, rhizoctonia solani and Rhizoctonia zeae was determined by plate-counter culture.
Firstly, inoculating Streptomyces nojirimensis (Streptomyces nojiriensis) strains 9-13 and 3 days on two sides of the edge of a PDA culture medium by streaking, inoculating a tested pathogenic bacteria cake with the diameter of 5mm in the center of a flat plate, culturing for 3-5 days at 25 ℃, and measuring the antibacterial zone width and the pathogenic bacteria diameter (Table 3) of the Streptomyces nojirimensis (Streptomyces nojiriensis) strains 9-13 on the tested pathogenic bacteria, wherein the tested pathogenic bacteria which are not connected with antagonistic bacteria are used as a control. The results showed that the strain 9-13 of Streptomyces nojirimensis (Streptomyces nojiriensis) had very strong antagonism against Rhizoctonia cerealis, rhizoctonia zeae and Rhizoctonia zeae (FIG. 2).
TABLE 3 antagonism of Streptomyces nojirimensis (Streptomyces nojiriensis) Strain 9-13 against pathogenic bacteria of different corn diseases
Embodiment III: inhibition of 4 pathogenic bacteria of different corn diseases by Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth.
Spores of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 activated by culture medium of Gao's No. 1 are inoculated into ISP2 liquid culture medium, the fermentation temperature is 26-28 ℃, the fermentation time is 7d, and a microporous filter membrane of 0.22 mu m is sterilized to obtain fermentation liquor for standby.
Adding 5mL of fermentation broth of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 into 50mLPDA culture medium, mixing, pouring into a culture dish, inoculating a test pathogenic bacteria cake with the diameter of 5mm into the center of a PDA culture medium plate, respectively culturing at 28 ℃ for 3-5d, and measuring colony diameter. The inhibition of hyphal growth of the 4 pathogenic bacteria by the broth was calculated using a blank PDA medium as a control (table 4). The results showed that the fermentation broth of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 had very strong inhibitory effect on the hyphal growth of Rhizoctonia cerealis, rhizoctonia zeae (FIG. 3).
TABLE 4 inhibition of hypha growth of 4 pathogenic bacteria of different corn diseases by Streptomyces nojirimensis (Streptomyces nojiriensis) Strain 9-13 fermentation broth
Embodiment four: inhibition of spore germination of Streptomyces zematosis and Leuconostoc zehnii by Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth.
Inoculating pathogenic bacteria cake with diameter of 5mm into PDA solid culture medium, culturing at 28deg.C in dark for 7d, and brushing with sterilized brushAfter mycelium on the surface of the culture medium is removed, the culture dish is placed under a black light lamp for culturing for 72 hours at 28 ℃ with moisture retention (the relative humidity is more than 90 percent), and the illumination time is 12 hours per day. Washing spores of Rhizoctonia cerealis and Rhizoctonia cerealis with sterile water, and adjusting their concentration to 1×10 4 And each mL.
Adding 20 mu L of the fermentation broth of the streptomyces nojirimi (Streptomyces nojiriensis) strain 9-13 in the third embodiment into 200 mu L of the spore suspension, placing the blank spore suspension at 28 ℃ for culturing for 3h and 2h respectively, and calculating the inhibition effect of the fermentation broth of the streptomyces nojirimi (Streptomyces nojiriensis) strain 9-13 on spore germination of the alternaria maize and the alternaria maize. The results showed that the fermentation broth of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 significantly inhibited spore germination of Rhizoctonia solani and Rhizoctonia cerealis and elongation of the germ tube of Rhizoctonia cerealis (FIG. 4).
Table 5 inhibition of Streptomyces nojirimensis (Streptomyces nojiriensis) Strain 9-13 fermentation broth on spore germination of Spot-forming bacteria of corn
Fifth embodiment: the application of the fermentation liquor of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 in preventing and treating corn northern leaf blight.
The test site is selected from XUNDANGvillage, fu Zhou, fujian, and the maize variety is obtained as white. The test set up 3 treatments, respectively: a.25% pyraclostrobin suspending agent 30 ml/mu, B.Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth, C.clear water control. Every 4 replicates, a total of 12 cells, random block arrangement, 30m per cell 2 45 liters of water are used per mu. Spraying and drug application control is started in the early stage of onset of the corn leaf spot, and spraying and drug application are carried out once at intervals of 7d, 2 times of drug application are carried out altogether, and onset conditions are investigated 10 days after last drug application.
Five spots were randomly taken per cell, and all leaves of 5 corn plants were investigated per spot, according to the following grading criteria.
The blade classification criteria are as follows:
level 0: no disease spots;
stage 1: the disease spots occupy less than 5 percent of the leaf area;
3 stages: the disease spots account for 6% -10% of the leaf area;
5 stages: the disease spots account for 11% -25% of the leaf area;
7 stages: the disease spots account for 26% -50% of the leaf area;
stage 9: the disease spots occupy more than 51% of the leaf area. Disease index = Σ (number of ears at each stage×number of relative stages)/(total ears investigated×9) ×100
Prevention effect (%) = [ (CK disease index-treatment disease index)/(CK disease index ] ×100)
Surface 6 Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation liquor field efficacy test for preventing and treating corn northern leaf blight
Experimental results show that the control effects of 30 ml/mu of the A.25% pyraclostrobin suspending agent and the B.nojirimonia (Streptomyces nojiriensis) strain 9-13 fermentation liquor on the corn northern leaf blight are 74.15% and 71.13% respectively, and the pyraclostrobin suspending agent and the Streptomyces nojirimonia (Streptomyces nojiriensis) strain are safe to corn and have no phytotoxicity. The control effect of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the corn northern leaf blight is not obvious on the 5% obvious level and 1% extremely obvious level compared with the control effect of 30 ml/mu of 25% pyraclostrobin suspending agent. Therefore, the control effect of the fermentation liquor of the streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the corn northern leaf blight is equivalent to the control effect of 30 ml/mu of the 25% pyraclostrobin suspending agent, and the application prospect is good.
Example six: the application of the fermentation liquor of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 in preventing and treating corn small spot disease.
The test site is selected from XUNDANGvillage, fuzhou, fujian, and the corn variety is Luo 31. The test set 3 treatments, 30 ml/mu of A.25% pyraclostrobin suspension, B.nojirimycin (Streptomyces)2.c. clear water control of the fermentation broth of the cs nojiriensis) strain 9-13. Every 4 replicates, a total of 12 cells, random block arrangement, 30m per cell 2 45 liters of water are used per mu. Spraying and drug application control is started in the early stage of the onset of the corn small spot disease, and spraying and drug application are carried out once at intervals of 7d, 2 times of drug application are carried out altogether, and the onset condition is investigated 10 days after the last drug application.
Five spots were randomly taken per cell, and all leaves of 5 corn plants were investigated per spot, according to the following grading criteria.
The blade classification criteria are as follows:
level 0: no disease spots;
stage 1: the disease spots occupy less than 5 percent of the leaf area;
3 stages: the disease spots account for 6% -10% of the leaf area;
5 stages: the disease spots account for 11% -25% of the leaf area;
7 stages: the disease spots account for 26% -50% of the leaf area;
stage 9: the disease spots occupy more than 51% of the leaf area.
Disease index = Σ (number of ears at each stage×number of relative stages)/(total ears investigated×9) ×100
Prevention effect (%) = [ (CK disease index-treatment disease index)/(CK disease index ] ×100)
Table 7 field efficacy test of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth for preventing and treating corn small spot disease
Experimental results show that the control effects of 30 ml/mu of the A.25% pyraclostrobin suspending agent and the B.nojirimonia (Streptomyces nojiriensis) strain 9-13 fermentation liquor on corn small spot disease are 73.52% and 71.17% respectively, and the obtained products are safe to corn and have no phytotoxicity. The control effect of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on corn small spot is not obvious on the 5% obvious level and 1% extremely obvious level compared with the control effect of 30 ml/mu of 25% pyraclostrobin suspending agent. Therefore, the control effect of the fermentation liquor of the streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the corn small spot disease is equivalent to the control effect of 30 ml/mu of the 25% pyraclostrobin suspending agent, and the method has good application prospect.
Embodiment seven: the application of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth in preventing and treating corn sheath blight is provided.
The corn variety in the test field is Min double-color No. 6, and the test is provided with 3 treatments, which are respectively: 30 ml/mu of 24% validamycin aqueous solution; B. streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth; C. and (5) comparing with clear water. Every 4 replicates, a total of 12 cells, random block arrangement, 30m per cell 2 45 liters of water are used per mu. The first application of the drug is carried out before the occurrence of diseases or in the early stage of the diseases from the stage of the corn jointing to the stage of the male pulling, the drug is mainly applied to the basal part of the stem, the drug is continuously applied for 3 times, the interval is 7 days, and the disease condition is investigated 10 days after the last drug.
Five spots per cell were randomly sampled, 20-30 strains per spot were investigated, and 100-150 strains per cell were investigated. The total number of plants was recorded and examined, and the number of plants at each stage was counted.
Grading standard:
level 0: the whole plant is asymptomatic;
stage 1: the 4 th leaf sheath and the lower leaf sheath below the lowest effective ear are ill;
3 stages: the 3 rd leaf sheath and the leaf sheaths below the lowest effective ear are ill;
5 stages: the 2 nd leaf sheath below the lowest effective ear and the leaf sheath below the lowest effective ear are ill;
7 stages: the lowest effective sub-spike leaf sheath 1 and below is diseased;
stage 9: the lowest effective ear and leaf sheath above it are ill.
Disease index = Σ (number of patients at each stage×number of patients at each stage)/(number of patients at each stage)/(total number of patients under investigation×9) ×100
Prevention effect (%) = [ (CK disease index-treatment disease index)/(CK disease index ] ×100)
Table 8 efficacy test of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth for preventing and treating corn sheath blight
Experimental results show that the control effects of 30 ml/mu of the A.24% validamycin aqueous solution and 9-13 of the B.nojirimycin (Streptomyces nojiriensis) strain on the corn sheath blight are 75.47% and 70.33% respectively, and the obtained products are safe to corn and have no phytotoxicity. The control effect of the fermentation liquor of the streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the corn sheath blight is not obvious on the basis of the control effect difference of 30 ml/mu with 24% validamycin aqueous solution at the obvious level of 5% and the extremely obvious level of 1%. Therefore, the control effect of the fermentation liquor of the streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the sheath blight of corn is equivalent to the control effect of 30 ml/mu of 24% validamycin aqueous solution, and the application prospect is good.
Claims (5)
1. The application of the Streptomyces nojirimensis strain in preventing and controlling corn diseases is characterized in that: the strain is Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13, and is preserved in China general microbiological culture Collection center (CGMCC) No.22798, and the preservation date is: 2021, 6, 28.
2. The use according to claim 1, characterized in that: the strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) has strong antagonism on pathogenic bacteria of corn disease, namely, corn big spot pathogen (Setosphaeria turcica), corn small spot pathogen (Cochliobolus heterostrophus), corn Rhizoctonia solani (Rhizoctonia solani) and corn Rhizoctonia zeae (Rhizoctonia zeae).
3. The application of the fermentation liquor of the streptomyces nojirimis strain in preventing and controlling corn diseases is characterized in that the fermentation liquor is obtained by inoculating 9-13 of the streptomyces nojirimis (Streptomyces nojiriensis) strain in claim 1 into an ISP2 liquid culture medium, fermenting at 26-28 ℃ for 7-8d, and sterilizing a microporous filter membrane.
4. Use according to claim 3, wherein the fermentation broth of the strain of streptomyces nojirimi (Streptomyces nojiriensis) 9-13 is used for inhibiting the growth of hyphae of the species chaetomium zeylanicum (Setosphaeria turcica), chaetomium zeylanicum (Cochliobolus heterostrophus), rhizoctonia solani (Rhizoctonia solani) and Rhizoctonia zeae (Rhizoctonia zeae).
5. The use according to claim 3, wherein the fermentation broth of the strain of streptomyces nojirimensis (Streptomyces nojiriensis) 9-13 is used for inhibiting spore germination of alternaria corn (Setosphaeria turcica) and alternaria corn (Cochliobolus heterostrophus).
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