CN115806913B - Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 and application thereof - Google Patents

Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 and application thereof Download PDF

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CN115806913B
CN115806913B CN202211504881.1A CN202211504881A CN115806913B CN 115806913 B CN115806913 B CN 115806913B CN 202211504881 A CN202211504881 A CN 202211504881A CN 115806913 B CN115806913 B CN 115806913B
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streptomyces
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nojirimensis
nojiriensis
agaricus bisporus
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CN115806913A (en
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杜宜新
石妞妞
陈福如
邱德柱
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Institute of Plant Protection of FAAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Abstract

The invention relates to a Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 and application thereof, belonging to the technical field of crop disease control. Streptomyces nojirimoniae strain 9-13 is preserved in China general microbiological culture Collection center (CGMCC) at 28 th month of 2021, and the preservation number is CGMCC No.22798. The strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) has antagonism to 3 kinds of verrucaria which cause the verrucaria agaricus bisporus, the fermentation liquor has inhibition effect to the hypha growth of 3 kinds of verrucaria agaricus, and the fermentation liquor has excellent control effect to the verrucaria agaricus bisporus and is safe to the agaricus bisporus.

Description

Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 and application thereof
Technical Field
The invention relates to the technical field of crop disease control, in particular to a Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 and application thereof.
Background
The agaricus bisporus is also called white mushroom, has good taste, and has both nutritive value and medicinal value, thus being an important edible and medicinal fungus. China is the biggest agaricus bisporus producing country in the world, and the yield of agaricus bisporus in China reaches 54% of the total yield worldwide in 2013.
Agaricus bisporus wart (wet bleb disease) caused by fungi of the genus wart (Mycogone) of the family Moniliaceae (Moniliaceae) occurs worldwide and has become one of the most important diseases of agaricus bisporus. At present, the prevention and treatment of wart spore mold has two characteristics: a commercial cultivar of agaricus bisporus lacking disease resistance; the verrucaria and the host agaricus bisporus belong to the same fungi, and the bactericide has an inhibition effect on both fungi, so that the safety of the agaricus bisporus needs to be considered when the efficacy of the bactericide is evaluated.
Agricultural antibiotics are used as biological pesticides with good development prospect, and have become an indispensable variety in pesticide structures in China. The Chinese is the agricultural antibiotic production country and the application country, and is also the country in which the agricultural antibiotic research is developed earlier. Research has been initiated as early as the 50 s of the 20 th century, with only slow walking, and great progress has been made until after the 70 s. The successful development of jinggangmycin, kasugamycin, agricultural resistance 120, polyoxin, blasticidin, principals kaolins, jiyang mycin, monensin, and gibberellins as plant growth regulators was continued. The Japanese Kagaku Kogyo Co Ltd.S. selects an antibiotic for sterilization of 50-100 mg.kg -1 Effective on mites and has ovicidal effect, and the residual period is more than 20 d; and has better control effects on rice blast, brown rot of peach, sclerotinia rot of cucumber and vegetable, powdery mildew of apple and melon, strawberry silk shell disease, gray mold of flower, cucumber and tobacco mosaic disease, etc. The mesogenic mycin (kejunkang) developed by the institute of biological control of Chinese academy of agricultural sciences is a novel agricultural antibiotic and is an N-glycoside alkaline water-soluble substance. The producing strain is Streptomyces lilacinus Hainan variety (Streptomyces 1avendulae var.hainanesis n.var.). The processed dosage form of the bacteria is a protective bactericide with wider bactericidal spectrum, and can prevent and treat rice, apples and vegetablesSome fungal diseases on crops such as citrus and ginger are effective.
The agricultural antibiotic is safe and nontoxic to human and livestock, does not pollute the environment, does not destroy ecological balance, and has remarkable social benefit and ecological benefit.
Disclosure of Invention
The invention aims at providing a Streptomyces nojirimi (Streptomyces nojiriensis) strain 9-13 which has antagonism to 3 pathogenic bacteria causing the agaricus bisporus wart, aiming at the problems that the occurrence of the agaricus bisporus wart prevents the safe production of agaricus bisporus industry, the safety of agaricus bisporus needs to be considered when a bactericide is used, and the like.
The invention further aims to provide application of fermentation liquor of the streptomyces nojirimis (Streptomyces nojiriensis) strain 9-13 in preventing and treating agaricus bisporus wart mould.
The aim of the invention is realized by the following technical scheme: streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13, wherein the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 is preserved in China general microbiological culture collection center (CGMCC) No.22798, and the preservation date is as follows: 2021, 6, 28.
The 16s rRNA sequence and rpoB gene sequence of the strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) are shown as SEQ ID NO. 1 and SEQ ID NO.2 respectively.
The strain 9-13 of Streptomyces nojirimensis (Streptomyces nojiriensis) has yellow mycelium cork and white aerial mycelium on the medium of Gao's first, and no soluble pigment is produced. Spore filaments are straight, flexible, hooked, loose and compact spiral, and spores are elliptic and cylindrical.
The physiological and biochemical characteristics of the Streptomyces nojirimoniae (Streptomyces nojiriensis) strain 9-13 are: melanin can be produced, and nitrate cannot be reduced; glucose, mannose, mannitol, lactose, galactose and the like can be utilized; alanine can be utilized; threonine, lysine and proline cannot be utilized.
Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 is antagonistic to harmful wart spore mold (Mycogone perniciosa), red wire wart spore mold (M.rosea) and M.xinjiangensis causing agaricus bisporus wart mold.
The application of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 in preventing and treating agaricus bisporus wart mold.
A method for preparing fermentation broth of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 comprises inoculating Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 into ISP2 liquid culture medium, fermenting at 26-28deg.C for 7-8d, and filtering to obtain fermentation broth.
The application of the fermentation broth of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 in preventing and controlling the agaricus bisporus wart mold.
The fermentation broth of the Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 is used for inhibiting the hypha growth of the agaricus bisporus wart mold pathogenic bacteria;
the pathogenic bacteria of the agaricus bisporus wartmold are harmful wart mold (Mycogone perniciosa), red silk wart mold (M.rosea) and M.xinjiangensis.
Compared with the prior art, the invention has the advantages that: the strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) has antagonism to 3 pathogenic bacteria causing the agaricus bisporus verrucosus and the fermentation liquor can obviously inhibit the hypha growth of 3 verrucosus and the prepared fermentation liquor has good control effect on the agaricus bisporus verrucosus and is safe to the agaricus bisporus.
In addition, the strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) is obtained from soil, is compatible with the soil environment in a harmonious manner, and has good application prospect.
Drawings
FIG. 1 shows the culture characteristics of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on medium of Gao's first.
FIG. 2 shows the opposing cultures of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 and 3 kinds of warts mold for the agaricus bisporus wart mold. And (3) injection: a is harmful wart spore (Mycogone perniciosa), B is red silk wart spore (M.rosea), C is M.xinjiangensis, and CK is control group.
FIG. 3 shows the inhibitory effect of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 fermentation broth on 3 kinds of wart mold of agaricus bisporus under in vitro conditions. And (3) injection: a is harmful wart spore (Mycogone perniciosa), B is red silk wart spore (M.rosea), C is M.xinjiangensis, and CK is control group.
Detailed Description
The present invention is described in detail below with reference to the drawings and examples of the specification:
embodiment one: isolation and identification of Streptomyces nojirimoniae (Streptomyces nojiriensis) strain 9-13.
1. Soil sample collection
3 parts of soil samples are collected from Jiangxi Jinggang mountains, soil on the surface is removed, soil samples in the depth of 5-20cm are collected, and the soil samples are marked and then brought back to a laboratory for natural air drying.
2. Isolation of actinomycetes
The separation was performed by plate dilution. Grinding air-dried soil sample with mortar, suspending 1g of sample in 9mL of sterile water, shaking at 40deg.C and 180rpm for 30min, standing for 5min, sequentially diluting 10 times, and respectively preparing into 10 -2 、10 -3 、10 -4 Respectively sucking 0.1mL of each suspension with different concentrations, adding the suspension into a modified HVA culture medium (adding potassium dichromate with the final concentration of 100-200 ppm) plate, uniformly coating, inverting the suspension, culturing and observing at 28 ℃, picking different single bacterial colonies after 5-7 days for streak purification, and preserving the purified bacterial strains in a refrigerator at-80 ℃ by adopting a glycerol method.
3. Identification of Streptomyces nojirimensis (Streptomyces nojiriensis) Strain 9-13
(1) Morphological feature observations
Streptomyces nojirimoniae (Streptomyces nojiriensis) strain 9-13 grew well on most media and did not produce soluble pigments (Table 1). Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 spores under an optical microscope are straight, flexible, hooked, loose and compact spiral, oval and cylindrical.
Table 1 culture characteristics of Streptomyces nojirimensis (Streptomyces nojiriensis) 9-13
(2) Physiological and biochemical characteristics
The characteristics of Streptomyces nojirimonia (Streptomyces nojiriensis) such as 9-13 nitrate reduction and carbon and nitrogen source utilization were measured by the method described in Streptomyces appraisal manual, and the results are shown in Table 2.
Table 2 physiological and biochemical characteristics of Streptomyces nojirimensis (Streptomyces nojiriensis) Strain 9-13
(3) Sequence analysis
After bacterial genome extraction kit extracts bacterial 9-13 genome DNA, respectively carrying out 16S rRNA and rpoB gene amplification, obtaining sequences with the total length of 1412bp and 859bp, submitting the obtained sequences to GenBank database for BLAST comparison analysis, and obtaining bacterial strain 9-13 as Streptomyces nojirimis (Streptomyces nojiriensis).
Embodiment two: antagonism of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 against 3 kinds of warts mold was measured.
Streptomyces nojirimis (Streptomyces nojiriensis) strain 9-13 was subjected to antagonistic assay against harmful wart spore mold, verrucaria rubra and M.xinjiangensis by plate-facing culture. Firstly, inoculating Streptomyces nojirimensis (Streptomyces nojiriensis) strains 9-13 on two sides of the edge of a PDA culture medium by streaking, inoculating a tested pathogenic bacteria cake with the diameter of 5mm in the center of a flat plate after 3d, and culturing at 25 ℃ for 5d to measure the antibacterial zone width and the pathogenic bacteria diameter (Table 3) of the Streptomyces nojirimensis (Streptomyces nojiriensis) strains 9-13 on the tested pathogenic bacteria, wherein the tested pathogenic bacteria which are not connected with antagonistic bacteria are used as a control. The results showed that Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 had very strong antagonism against all of the species Pythium ultimum, pythium erythropolis and M.xinjiangensis (FIG. 2).
Table 3 antagonism of streptomyces nojirimensis strains 9-13 against 3 warts
Embodiment III: inhibition of 3 kinds of verrucaria bacteria by Streptomyces nojirimoniae (Streptomyces nojiriensis) strain 9-13 fermentation broth.
Spores of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 activated by culture medium of Gao's No. 1 are inoculated into ISP2 liquid culture medium, the fermentation temperature is 26-28 ℃, the fermentation time is 7-8d, the fermentation liquor is obtained by filtration, and then a microporous filter membrane of 0.22 mu m is sterilized for standby.
Adding 5mL of fermentation broth of Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 into 50mL of PDA culture medium, mixing, pouring into a culture dish, inoculating a pathogenic bacteria cake with the diameter of 5mm into the center of a PDA culture medium plate, culturing at 25 ℃ for 5d, and measuring colony diameter. The inhibition of the growth of hyphae of 3 warts by the broth was calculated using a blank PDA medium as a control (table 4). The results showed that the fermentation broth of Streptomyces nojirimis (Streptomyces nojiriensis) strain 9-13 has a strong inhibitory effect on the hyphal growth of both Mibropsis, heteromyces rubellus and M.xinjiangensis (FIG. 3).
TABLE 4 inhibition of hyphal growth of Streptomyces verrucosus by Streptomyces nojirimensis Strain 9-13 fermentation broth
Embodiment four: the application of the fermentation liquor of the strain 9-13 of the Streptomyces nojirimis (Streptomyces nojiriensis) in preventing and controlling the wart mold of agaricus bisporus.
Culturing the harmful wart, red wart and M.xinjiangensis at 25deg.C for 5d, taking out bacterial cake at colony edge with hole puncher with diameter of 5mm, transferring bacterial cake into PDB liquid culture medium, culturing in dark shaking at 25deg.C for 7d at 150r/min, detecting chlamydospore concentration with blood cell counting plateMaking into a concentration of 1×10 5 The spores were mixed into a suspension per mL for further use.
The test site is selected from XUN Parcun, fujian province, and the agaricus bisporus is W192. The test set up 3 treatments, respectively: a.50% prochloraz manganese chloride WP1.0 g/square meter, B.Streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13, C.clear water control. Every 4 replicates, a total of 12 cells, random block arrangement, 4m per cell 2 . The liquid medicine with the concentration to be tested is quantitatively sprayed on quantitative soil grains to be covered (about 25kg of the soil for covering each square meter of mushroom bed, 1.5L of the liquid medicine is sprayed), uniformly stirred, the water content of the soil is kept to be about 70%, the soil is covered for 24 hours after the soil is covered by a plastic film, and then 100mL of the spore mixed suspension is inoculated in each cell. And (3) respectively carrying out second application after the soil is covered for 7d, and uniformly spraying the same dosage of liquid medicine on soil particles on the bed surface until the soil particles are wet and do not flow into the bacteria material. Investigation is started from the first tide mushroom harvesting, 3 tide mushrooms are investigated in total, a full-cell investigation mode is adopted, the number of diseased mushrooms or the number of clusters (the number of the diseased mushrooms or the number of clusters is more than or equal to 1 cm) on the culture material is investigated and recorded during each harvesting, the number of the diseased mushrooms in each cell is accumulated, and the control effect is calculated.
Control effect (%) = [ (control area disease mushroom count-treatment area disease mushroom count)/control area disease mushroom count ] ×100
Field efficacy test of fermentation broth of Streptomyces nojirimensis strain 9-13 for preventing and treating agaricus bisporus wart mold
Experimental results show that the fermentation liquor of A.50% prochloraz manganese chloride WP1.0 g/square meter and B.nojirimonia (Streptomyces nojiriensis) strain 9-13 has the control effects of 77.53% and 75.62% on agaricus bisporus wartmold respectively, and is safe to agaricus bisporus and has no phytotoxicity. The control effect of the fermentation liquor of the streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the agaricus bisporus wartmold is not obvious on the obvious level of 5% and the extremely obvious level of 1%, and the control effect is not obvious from the control effect of 1.0 g/square meter of 50% prochloraz manganese WP. Therefore, the control effect of the fermentation liquor of the streptomyces nojirimensis (Streptomyces nojiriensis) strain 9-13 on the agaricus bisporus wartmold is equivalent to the control effect of 50% prochloraz manganese WP1.0 g/square meter, and the method has good application prospect.
The 16s rRNA sequence and rpoB gene sequence of the strain 9-13 of the Streptomyces nojirimensis (Streptomyces nojiriensis) are shown as SEQ ID NO. 1 and SEQ ID NO.2 respectively.
SEQ ID NO:1
TGCGGCGTGCTTACCATGCAGTCGAACGATGAACCTCCTTCGGGAGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTTCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATATGACACGGGGTCGCATGATCTCCGTGTGGAAAGCTCCGGCGGTGAAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGGTAGTGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCAGGCTAGAGTTCGGTAGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTGGGCGACATTCCACGTCGTCCGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAAACCGTGGAGACACGGTCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCTTTCGGGGTGATGGGGACTCACAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGAGGGAATCGTCGAAGG
SEQ ID NO:2
GTACGGCTAAAAAAGCGCGCCTGTCGGCGCTGGGCCCCGGCGGTCTGTCCCGTGAGCGGGCCGGCTTCGAGGTCCGTGACGTTCACCCGTCGCACTACGGCCGCATGTGCCCGATCGAGACCCCTGAAGGCCCGAACATCGGTCTGATCGGCTCGCTCGCCTCCTACGGTCGCGTCAACGCGTTCGGTTTCGTCGAGACCCCGTACCGCAAGGTCATCGACGGTGTCGTCACCGACGAGGTCGACTACCTGACGGCCGACGAGGAAGACCGCTTCGTCATCGCCCAGGCCAACGCCGGCCTGTCCGAGGACATGCGCTTCACCGAGAACCGCGTGCTGGTGCGCCGTCGTGGCGGCGAGATCGACTACATCGCCGGCGACGACGTCGACTACATGGACGTCTCCCCGCGCCAGATGGTGTCCGTCGCGACCGCGATGATCCCCTTCCTGGAGCACGACGACGCCAACCGTGCCCTCATGGGCGCGAACATGATGCGCCAGGCCGTTCCGCTCATCAAGGCGGAGGCCCCGCTCGTCGGCACCGGCATGGAGTACCGCTGTGCGGTCGACGCCGGTGACTCGATCCGTGCGGAGAAGGACGGTGTCGTCCAGGAGGTCTCGGCCGACTACATCACCGTCGCCAACGACGACGGCACGTACACCACGTACCGCGTCGCCAAGTTCTCCCGCTCGAACCAGGGCACCTCGGTCAACCAGAAGGTCATCGTCAACGAGGGTGACCGGATCATCGAGTCCCAGGTCCTCGCCGACGGTCCCGCGACCGAAGAGGGCGAAATGG
CCCTCGGCAAGAACCTGCTCGTCGCGTTCATGCCGTGGGAGGGCCCAACT
TATACAAAGGA。

Claims (6)

1. Streptomyces nojirimensisStreptomyces nojiriensis) Strains 9-13, characterized in that:
the Streptomyces nojirimensis isStreptomyces nojiriensis) The strain 9-13 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.22798 and the preservation date: 2021, 6, 28.
2. The Streptomyces nojirimensis according to claim 1Streptomyces nojiriensis) The application of the strain 9-13 in preventing and controlling agaricus bisporus wart spore mold.
3. The Streptomyces nojirimensis according to claim 1Streptomyces nojiriensis) The preparation method of the fermentation broth of the strain 9-13 is characterized by comprising the following steps: streptomyces nojirimensisStreptomyces nojiriensis) The strain 9-13 is inoculated in ISP2 liquid culture medium, the fermentation temperature is 26-28 ℃, the fermentation culture time is 7-8d, and the microporous filter membrane is sterilized to obtain fermentation liquor.
4. A Streptomyces nojirimensis prepared by the method of claim 3Streptomyces nojiriensis) Fermentation broth of strain 9-13.
5. The Streptomyces nojirimensis according to claim 4Streptomyces nojiriensis) The application of the fermentation broth of the strain 9-13 is characterized in that: streptomyces nojirimensisStreptomyces nojiriensis) The application of the fermentation broth of the strain 9-13 in preventing and controlling agaricus bisporus wart spore mold.
6. The Streptomyces nojirimensis according to claim 5Streptomyces nojiriensis) The application of the fermentation broth of the strain 9-13 is characterized in that: streptomyces nojirimensisStreptomyces nojiriensis) The fermentation broth of the strain 9-13 is used for inhibiting the hypha growth of the agaricus bisporus wart spore mold pathogenic bacteria;
the pathogenic bacteria of the agaricus bisporus verrucosum spore mold is harmful verrucosum @, and the agaricus bisporus verrucosum spore mold is a harmful verrucosum @Mycogone perniciosa) Verruca rhodosporidium (L.) SpM. rosea) AndM. xinjiangensis。
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