CN105367305A - Compound microbial fertilizer with disease and pest resistance as well as preparation method and application of compound microbial fertilizer - Google Patents

Compound microbial fertilizer with disease and pest resistance as well as preparation method and application of compound microbial fertilizer Download PDF

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Publication number
CN105367305A
CN105367305A CN201510988255.8A CN201510988255A CN105367305A CN 105367305 A CN105367305 A CN 105367305A CN 201510988255 A CN201510988255 A CN 201510988255A CN 105367305 A CN105367305 A CN 105367305A
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disease
fertilizer
insect resistance
organic
parts
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黄春艳
穆光远
杜怡然
韦春近
毛莲花
张敏
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Shenzhen Batian Ecotypic Engineering Co Ltd
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Shenzhen Batian Ecotypic Engineering Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

The invention provides compound microbial fertilizer with disease and pest resistance. The compound microbial fertilizer comprises raw materials in parts by weight as follows: 5-10 parts of a compound microbial agent, 50-76 parts of yeast powder, 12-20 parts of an auxiliary material, 10-18 parts of waste molasses and 1-3 parts of paecilomyces lilacinus, wherein the compound microbial agent comprises bacillus subtilis, bacillus amyloliquefaciens, bacillus megaterium and bacillus licheniformis. A preparation method comprises following steps: the yeast powder and the auxiliary material are mixed, sieved and sterilized for preparation of organic powder; then the organic powder and paecilomyces lilacinus are mixed sufficiently for granulation, and organic granules containing paecilomyces lilacinus are prepared; the prepared compound microbial agent and the waste molasses are mixed, and a thick liquid is prepared and sprayed to the surfaces of the organic granules. A multi-level antagonism isolating mode is adopted, so that the components not only can resist diseases and kill insects, but also can promote mutually and produce a synergistic effect for promotion of plant growth and increase of crop yield. The invention further provides an application of the compound microbial fertilizer with disease and pest resistance in crop growth.

Description

Composite microbiological fertilizer of disease and insect resistance and its preparation method and application
Technical field
The invention belongs to technical field of biological fertilizer, particularly relate to composite microbiological fertilizer of a kind of disease and insect resistance and its preparation method and application.
Background technology
Fertilizer is the promotor helping plant-growth, but the specific absorption of plant to fertilizer is limited, it absorbs and loses and deposit, and the fertilizer of loss on the one hand causes farmland, the pollution of underground water and the waste of resource, on the other hand crop is because of cannot efficient absorption fertilizer and cause the decline of Yield and qualities.From fertilizer self, extend the rate of release of self, making plant can effectively absorb with decreasing pollution and growth promoting effects is the target that technical field of fertilizers is constantly pursued.
At present, due to a large amount of uses of chemical fertilizer and agricultural chemicals, the unreasonable collocation of fertilizer, bio-fertilizer and using, Soil structure is caused to destroy serious, planting environment is polluted, and plant growing area reduces, soil fertility and soil fertility deficiency, disease and pest takes place frequently, and seriously decreases output and the peasant planting income of plant.The large agricultural country in China and even the world more and more payes attention to the pollution problem of chemical fertilizer to planting environment, and praise highly green agriculture and the ecological agriculture, biological control also becomes focus.
Have bacterium class as bacillus category, pseudomonas class, edaphic bacillus class, Azobacter and root nodule mushroom etc. for the microorganism of anti-soil-borne disease as blight, southern blight, gray mold etc., Mycophyta is as Trichoderma, green muscardine fungus, Paecilomyces lilacinus etc.The mechanism of action of these biocontrol microorganisms is divided into 4 classes: one is antagonistic action, refers to microorganism and produces growth or the development of the harmful pathogen of antimicrobial substance suppression by assimilation or directly kill pathogen.If this kind of microbial host bacillus category is as Bacillus licheniformis, bacillus amyloliquefaciens, subtilis and actinomycetes etc.; Two is induction of resistance, and the mechanism of action of induction of resistance comprises: (1) produces the lower molecular weight chemical substance of the disease-resistant original as phytoalexin, and xylogen, is rich in the glycoprotein etc. of light proline(Pro); (2) some lytic enzyme and oxydase etc. are induced, as chitinase, peroxidase.Paecilomyces lilacinus can conciliate amylase by self secretion chitinase, has potent killing action to nematode; (3) generation etc. of pathogenesis-related proteins is induced.Three is superparasitisms, the phytopathy original by the phenomenon of other biological parasitism hyperparasitism, these superparasitism biologies can phytoparasite pathogenic fungi, bacterium and plant nematode etc.As Trichoderma is wound around phytophthora silk by mycelia, the structure producing haustorium or similar haustorium with point of contact penetrates the mycelia of phytophthora, and phytophthora silk is cleared up and vacuolization, protoplastis focusing, and finally condenses.Four is competition and colonized action, Biocontrol microorganism is grabbed by competition nutritive substance and Advantageous sites, utilize rhizosphere nutrition and root exudates amount reproduction, reduce the necessary nutritive substance of pathogenic bacteria survival and reproduction, stop and reduce the foundation of pathogenic bacteria at Advantageous sites.
The microorganism fertilizer circulated in the market and biological organic fertilizer of a great variety, but effect is not remarkable.Major cause one is that the content of microorganisms added is low, and probiotics quantity is few, function singleness; Two is that microbe species compatibility is unreasonable, mutually produces antagonistic action and misses one's aim.
Summary of the invention
An object of the present invention is to overcome deficiency of the prior art, provides a kind of composite microbiological fertilizer of disease and insect resistance, can improve the application effect of calcareous fertilisers.
The composite microbiological fertilizer of disease and insect resistance provided by the invention, comprises the raw material of following weight parts:
Complex micro organism fungicide 5-10 part, yeast powder 50-76 part, auxiliary material 12-20 part, waste molasses 10-18 part, Paecilomyces lilacinus 1-3 part.
Preferred as composite microbiological fertilizer of the present invention, described complex micro organism fungicide can be composited by weight percent 20-30% subtilis, 15-25% bacillus amyloliquefaciens, 10-20% bacillus megaterium and 25-35% Bacillus licheniformis.
Preferred as composite microbiological fertilizer of the present invention, described yeast powder includes following main component: N P and K 12-20%, medium trace element 1-3%, amino acid 3.5-6%, water-insoluble lower than 0.5%, organic 60-75%, moisture 5%,
Preferred as composite microbiological fertilizer of the present invention, described yeast powder can be the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid or sugar refinery waste liquid through concentrate drying.
Preferred as composite microbiological fertilizer of the present invention, described yeast powder PH6.8-7.4.
Preferred as composite microbiological fertilizer of the present invention, described auxiliary material comprises following at least one of becoming thoroughly decomposed in material: plant residue, changing food waste, human and animal excreta, mud.
Composite microbiological fertilizer of the present invention is composited by multiple-microorganism genus bacillus, this several genus bacillus compound can not produce antagonistic action, fight for nutrient by the quick Growth and reproduction of Useful Bacillus, occupy space, produce antibiotic agents, can produce multi-level disease and insect resistance effect, greatly inhibit the Growth and reproduction of harmful levels of pathogens, and these genus bacillus can secrete some growth-promoting class materials, Promoting plant growth, improves the resistibility of plant to soil-borne disease.Also help with yeast powder, auxiliary material and Paecilomyces lilacinus and complex microorganism interworking in instant component, achieve and root nematode contact is killed, therefore have and kill the effects such as line, disease-resistant, growth-promoting, volume increase at many levels, fertilizer is possessed several functions, be conducive to the performance of microbial function, greatly improve ability and growth-promoting, the effect of increasing production of fertilizer disease and insect resistance.
Present invention also offers the preparation method of the composite microbiological fertilizer of disease and insect resistance, comprise the steps:
Yeast powder and auxiliary material are sieved according to weight part mixing, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
Preparation complex micro organism fungicide;
By weight described organic powder thing and Paecilomyces lilacinus are fully mixed, then granulation, make the organic granular containing Paecilomyces lilacinus;
Thick liquid is made after fully being mixed by weight with waste molasses by described complex micro organism fungicide, then described organic granular liquid spray prepared in above-mentioned steps is surperficial, namely can be made into living bacteria count 0.2-5 hundred million/gram, npk nutrient 6-12%, the complex microorganism granulated fertilizer of moisture less than 10%.
Paecilomyces lilacinus is blended in organic granular by preparation process of the present invention, the competitive antagonism of Paecilomyces lilacinus and genus bacillus can be avoided, effectively ensure that the activity of Paecilomyces lilacinus and expand numerous, being conducive to improving the disease resistance effect of fertilizer and the stability of fertilizer quality.
The application of composite microbiological fertilizer in plant growth of the composite microbiological fertilizer that present invention also offers above-mentioned disease and insect resistance and the disease and insect resistance that adopts above-mentioned preparation method to prepare, can greatly reduce or avoid the disease and pest in crop-planting process, improve soil microenvironment, reduce pesticide residue, reduce the usage quantity of chemical fertilizer, there is the effect of biological control, be conducive to environmental protection.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The present invention provide firstly a kind of composite microbiological fertilizer of disease and insect resistance, comprises the raw material of following weight parts:
Complex micro organism fungicide 5-10 part, yeast powder 50-76 part, auxiliary material 12-20 part, waste molasses 10-18 part, Paecilomyces lilacinus 1-3 part.
As preferably of the present invention, described complex micro organism fungicide is composited by weight percent 20-30% subtilis, 15-25% bacillus amyloliquefaciens, 10-20% bacillus megaterium and 25-35% Bacillus licheniformis.Wherein said subtilis can secrete indolylacetic acid, indolylacetic acid is a kind of plant growth hormones, the organic matter decomposition in soil can be impelled to become soil ulmin, great raising soil fertility, the breeding of pathogenic bacteria in soil and the invasion and attack to plant root can also be suppressed simultaneously, reduce plant soil-borne diseases, reduce and prevent the generation of various mushroom disease, improve disease-resistant crops ability; Described bacillus amyloliquefaciens can produce amylase and proteolytic enzyme and secrete multiple antibacterial substance, high starch and the coarse-fibred macromolecular substance of height can be degraded for plant utilization, the antibacterial substance of secretion can destroy the structure of harmful pathogenic bacteria, suppress growth of pathogenic bacteria, there is the function of extensive Antifungi and bacterium; Immobilized phosphorus can be changed into the phosphorus that plant absorption utilizes by described bacillus megaterium, effectively can improve the specific absorption of Soil Phosphorus, improve soil microenvironment, alleviate disease, reduces pesticide residue; Described Bacillus licheniformis can produce multiple antibacterial substance equally, kills pathogenic bacterium, body can be impelled to produce antibacterial substance, and the biology with uniqueness takes oxygen mechanism of action by force, and the growth and breeding of pathogenic bacterium can be suppressed also to have the effect of nematicide simultaneously.
Described Paecilomyces lilacinus can secrete chitinase and proteolytic enzyme, has contact kill function to root nematode, reduces the harm of nematode in root system, and then can promote the growth of root system of plant and plant nutrition organ.Meanwhile, the decomposition of many chemical polymerization things (as agricultural chemicals) can also be promoted, there is certain environmental protection effect.
Described yeast powder includes following main component: N P and K 12-20%, medium trace element 1-3%, amino acid 3.5-6%, water-insoluble lower than 0.5%, organic 60-75%, moisture 5%; Can be the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid or sugar refinery waste liquid through concentrate drying, its PH controls between 6.8-7.4.Described yeast powder nutritive substance enriches, and N-P-K content is moderate, and organic content is high, good water solubility, can improve speed and the efficiency of antimicrobial absorbent utilization, be applicable to the nourishing body doing microorganism and plant.Meanwhile, because above-mentioned yeast powder is the byproduct that amino acid, monosodium glutamate, yeast or sugar refinery process, the utilization of waste material of above-mentioned processing enterprise can be realized, reduce fertilizer material cost, thus reduce composite microbiological fertilizer holistic cost of the present invention.
A kind of byproduct when described waste molasses is sugaring, sugared content is high, compares thickness, is not only the nutrition that microbial growth metabolism provides enough, simultaneously or a kind of tackiness agent of microbial envelope.These raw materials and complex microorganism interworking, be conducive to the performance of microbial function, greatly improves ability and growth-promoting, the effect of increasing production of fertilizer disease and insect resistance.Similarly, because waste molasses source cost is low, the raw materials cost of fertilizer can also effectively be lowered.
Described auxiliary material can comprise following at least one of becoming thoroughly decomposed in material: plant residue, changing food waste, human and animal excreta, mud, not only can be crop and nutrition is provided, and the activity of soil microorganisms can be promoted, improve soil physics, chemistry and biological nature, increase soil fertility, and described in become thoroughly decomposed material source easily, cost is low.
The composite microbiological fertilizer of disease and insect resistance provided by the invention, microbe species is many, function is collaborative to be promoted, is fought for nutrient, is occupied space, produce antibiotic agents by the quick Growth and reproduction of useful genus bacillus, nematicide, Anti-bacterium, greatly inhibit the Growth and reproduction of harmful levels of pathogens, disease-resisting, insecticidal Be very effective, these genus bacillus can secrete some growth-promoting class materials simultaneously, Promoting plant growth, improves the resistibility of plant to soil-borne disease.In component, Paecilomyces lilacinus can secrete chitinase and proteolytic enzyme, kills root nematode contact.Therefore, the present invention adopts biological pest control to have the component theory increasing soil fertility concurrently, have and kill the effects such as line, disease-resistant, growth-promoting, volume increase at many levels, reduce and prevent the generation of various mushroom disease, effectively reduce plant soil-borne diseases, improve the resistance against diseases of crop, effectively improve soil microenvironment, enhance crop root to fertilizer receptivity, thus be conducive to improving the utilization ratio of fertilizer and the stability of fertilizer quality, promote plant growth, improve the quality of crop, there is wide application market.In addition, biological control is the prevention and controls most in current disease control with application prospect, meets environmental protection and health idea.
The invention provides the method for the composite microbiological fertilizer preparing above-mentioned disease and insect resistance, comprise the steps:
Yeast powder and auxiliary material are sieved according to weight part mixing, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
Preparation complex micro organism fungicide: subtilis, bacillus amyloliquefaciens, bacillus megaterium and Bacillus licheniformis are combined into microbiobacterial agent in proportion;
By weight described organic powder thing and Paecilomyces lilacinus are fully mixed, then use granulator granulation, make the organic granular containing Paecilomyces lilacinus;
Thick liquid is made after fully being mixed by weight with waste molasses by the complex micro organism fungicide of above-mentioned preparation, then this thick liquid is sprayed on organic granular surface prepared by above-mentioned steps by coating machine injection system, namely makes the complex microorganism granulated fertilizer that microorganism is coated.
Described complex microorganism granulated fertilizer living bacteria count 0.2-5 hundred million/gram after preparation, npk nutrient 6-12%, moisture is below 10%.
In the above-mentioned preparation process of the present invention, Paecilomyces lilacinus is blended in organic granular, other complex micro organism fungicide coatings are on organic granular surface, the competitive antagonism of Paecilomyces lilacinus and genus bacillus can be avoided, effectively ensure that the activity of Paecilomyces lilacinus and expand numerous, being conducive to improving the utilization ratio of fertilizer and the stability of fertilizer quality.Preparation process of the present invention is simple, and cost is low, is easy to realize.
The composite microbiological fertilizer product of disease and insect resistance provided by the invention, can be applicable to various crop, the root knot nematode that especially can greatly reduce or avoid to produce in cash crop and growing vegetables process, the harm of Cyst nematode, to the sprouting of seed and growth of seedling, there is promoter action, can realize that seedling is complete, Miao Lv, Miao Zhuan, improve crop yield and quality, and can soil microenvironment be improved, reduce pesticide residue, reduce the usage quantity of chemical fertilizer, have environmental protection effect concurrently.
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
1) yeast powder 50 parts is selected, auxiliary material 12 parts, waste molasses 10 parts, complex micro organism fungicide 5 parts, Paecilomyces lilacinus 1 part.
Wherein said yeast powder is the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid, sugar refinery waste liquid through concentrate drying, its composition consists of N P and K 13%, medium trace element 2%, amino acid 4.8%, water-insoluble 0.2%, organic 75%, moisture 5%, pH about about 7.
Described auxiliary material is following at least one of becoming thoroughly decomposed in material: plant residue, changing food waste, human and animal excreta or mud.
Described complex micro organism fungicide is composited by weight percent 25% subtilis, 25% bacillus amyloliquefaciens, 15% bacillus megaterium and 35% Bacillus licheniformis.
2) yeast powder of above-mentioned configuration and auxiliary material are sieved according to weight part mixing, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
3) subtilis, bacillus amyloliquefaciens, bacillus megaterium and Bacillus licheniformis are combined into microbiobacterial agent in proportion;
4) by step 2) the organic powder thing that configures and Paecilomyces lilacinus fully mix by weight, then carry out granulation, make the organic granular containing Paecilomyces lilacinus;
5) by step 3) in complex micro organism fungicide fully mix by weight with waste molasses after make thick liquid, then by liquid spray in step 4) in organic granular surface, make the complex microorganism granular fertilizer that microorganism is coated, living bacteria count 0.2 hundred million/gram, npk nutrient 6%, moisture less than 10%, validity period more than 6 months.
Embodiment 2:
1) yeast powder 60 parts is selected, auxiliary material 16 parts, waste molasses 14 parts, complex micro organism fungicide 8 parts, Paecilomyces lilacinus 2 parts.
Wherein said yeast powder is the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid, sugar refinery waste liquid through concentrate drying, its composition consists of N P and K 15%, medium trace element 2.6%, amino acid 6%, water-insoluble 0.4%, organic 71%, moisture 5%, pH about about 6.8.
Described auxiliary material is following at least one of becoming thoroughly decomposed in material: plant residue, changing food waste, human and animal excreta or mud.
Described complex micro organism fungicide is composited by weight percent 30% subtilis, 20% bacillus amyloliquefaciens, 20% bacillus megaterium and 30% Bacillus licheniformis.
2) yeast powder of above-mentioned configuration and auxiliary material are sieved according to weight part mixing, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
3) subtilis, bacillus amyloliquefaciens, bacillus megaterium and Bacillus licheniformis are combined into microbiobacterial agent in proportion;
4) by step 2) the organic powder thing that configures and Paecilomyces lilacinus fully mix by weight, then carry out granulation, make the organic granular containing Paecilomyces lilacinus;
5) by step 3) in complex micro organism fungicide fully mix by weight with waste molasses after make thick liquid, then by liquid spray in step 4) in organic granular surface, make the complex microorganism granular fertilizer that microorganism is coated, living bacteria count 1.0 hundred million/gram, npk nutrient 8%, moisture less than 10%, validity period more than 6 months.
Embodiment 3:
1) yeast powder 78 parts is selected, auxiliary material 20 parts, waste molasses 18 parts, complex micro organism fungicide 10 parts, Paecilomyces lilacinus 3 parts.
Wherein said yeast powder is the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid, sugar refinery waste liquid through concentrate drying, its composition consists of N P and K 20%, medium trace element 3%, amino acid 3.7%, water-insoluble 0.3%, organic 68%, moisture 5%, pH about about 7.2.
Described auxiliary material is following at least one of becoming thoroughly decomposed in material: plant residue, changing food waste, human and animal excreta or mud.
Described complex micro organism fungicide is composited by weight percent 30% subtilis, 25% bacillus amyloliquefaciens, 20% bacillus megaterium and 25% Bacillus licheniformis.
2) yeast powder of above-mentioned configuration and auxiliary material are sieved according to weight part mixing, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
3) subtilis, bacillus amyloliquefaciens, bacillus megaterium and Bacillus licheniformis are combined into microbiobacterial agent in proportion;
4) by step 2) the organic powder thing that configures and Paecilomyces lilacinus fully mix by weight, then carry out granulation, make the organic granular containing Paecilomyces lilacinus;
5) by step 3) in complex micro organism fungicide fully mix by weight with waste molasses after make thick liquid, then by liquid spray in step 4) in organic granular surface, make the complex microorganism granular fertilizer that microorganism is coated, living bacteria count 500,000,000/gram, npk nutrient 12%, moisture less than 10%, validity period more than 6 months.
Comparative example 1:(organic matter material is different and do not add Paecilomyces lilacinus)
1) organic 60 parts are selected, N P and K 10 parts, complex micro organism fungicide 10 parts.
Described organic matter is two or more composition in humic acids, peat soil, the peat composed of rotten mosses, straw powder, amino acid etc.
Described complex micro organism fungicide is composited by weight percent 25% subtilis, 25% bacillus amyloliquefaciens, 15% bacillus megaterium and 35% Bacillus licheniformis.
2) above-mentioned subtilis, bacillus amyloliquefaciens, bacillus megaterium, Bacillus licheniformis are combined into microbiobacterial agent in proportion;
3) carry out granulation, oven dry by traditional biological fertilizer method after fully being mixed with organic matter by microbiobacterial agent, make complex microorganism granulated fertilizer, living bacteria count 1.0 hundred million/gram, npk nutrient 8%, moisture less than 10%, validity period more than 6 months.
Comparative example 2:(organic matter material is identical, does not add Paecilomyces lilacinus)
1) yeast powder 50 parts is selected, auxiliary material 12 parts, waste molasses 10 parts, complex micro organism fungicide 10 parts.
Described yeast powder is the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid, sugar refinery waste liquid through concentrate drying, its composition consists of N P and K 15%, medium trace element 2.6%, amino acid 6%, water-insoluble is lower than 0.4%, organic 71%, moisture 5%, pH6.8.
Described auxiliary material is the material that becomes thoroughly decomposed: at least one in plant residue, changing food waste, human and animal excreta or mud.
Described complex micro organism fungicide is by weight percent 30% subtilis, 20% bacillus amyloliquefaciens, 20% bacillus megaterium and 30% Bacillus licheniformis.
2) yeast powder of above-mentioned configuration, auxiliary material and waste molasses are proportionally mixed and sieve, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
3) subtilis, bacillus amyloliquefaciens, bacillus megaterium and Bacillus licheniformis are combined into microbiobacterial agent in proportion;
4) by step 2) in organic powder thing and step 3) in complex micro organism fungicide fully mix, then carry out granulation, manufacture composite microbiological fertilizer, living bacteria count 1.0 hundred million/gram, npk nutrient 8%, moisture 10%.
Comparative example 3:(preparation method is different)
1) yeast powder 80 parts is selected, auxiliary material 25 parts, waste molasses 20 parts, complex micro organism fungicide 15 parts.
Described yeast powder is the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid, sugar refinery waste liquid through concentrate drying.Its composition consists of N P and K 20%, medium trace element 3%, amino acid 3.7%, water-insoluble lower than 0.3%, organic 68%, moisture 5%, pH7.2.
Described auxiliary material is following at least one of becoming thoroughly decomposed in material: plant residue, changing food waste, human and animal excreta or mud.
Described complex micro organism fungicide is composited by weight percent 20% subtilis, 20% bacillus amyloliquefaciens, 15% bacillus megaterium, 25% Bacillus licheniformis and 20% Paecilomyces lilacinus.
2) yeast powder of above-mentioned configuration, auxiliary material and waste molasses are proportionally mixed and sieve, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
3) subtilis, bacillus amyloliquefaciens, bacillus megaterium, Bacillus licheniformis and Paecilomyces lilacinus are combined into microbiobacterial agent in proportion;
3) by 1) in organic powder thing and 2) in complex micro organism fungicide fully mix, then carry out granulation, manufacture complex micro organism fungicide, living bacteria count 500,000,000/gram, npk nutrient 15%, moisture 20%.
Above-mentioned several fertilizer is used for the plantation of banana, Comparative result is as shown in the table:
Above-mentioned several fertilizer is used for the plantation of pimento, Comparative result is as shown in the table:
Above-mentioned chart is known, in the planting experiment of banana and pimento, comparative example 1 adopts conventional organic matter material and does not add Paecilomyces lilacinus, its blight prevention effect and root nematode control rate low, banana and pimento effect of increasing production not good, the fruit maturation time and fruit dimension c content and general fertilizers be as good as; Although and comparative example 2 organic matter material and embodiment 1,2,3 identical, owing to not adding Paecilomyces lilacinus, the more conventional organic fertilizer of its blight prevention effect, root nematode control rate, fruit dimension c content slightly improves; Comparative example 3 raw material and embodiment 1,2,3 identical, but owing to having mixed with complex micro organism fungicide during Paecilomyces lilacinus during preparation, can see thus, its blight prevention effect, root nematode control rate, fruit dimension c content and the embodiment of the present invention 1,2,3 still have a certain distance.So, adopt composite microbiological fertilizer of the present invention, effectively can reduce disease and pest, increase crop yield, shorten the fructescence, improve fruit vitamin contents, improve product quality.
In sum; be only the part of present pre-ferred embodiments shown in the above embodiment of the present invention, the present invention can not be limited to this, under the condition not departing from marrow of the present invention; any amendment that those skilled in the art do, equivalent replacement and improvement etc., all belong to protection scope of the present invention.

Claims (9)

1. a composite microbiological fertilizer for disease and insect resistance, is characterized in that, comprises the raw material of following weight parts:
Complex micro organism fungicide 5-10 part, yeast powder 50-76 part, auxiliary material 12-20 part, waste molasses 10-18 part, Paecilomyces lilacinus 1-3 part.
2. the composite microbiological fertilizer of disease and insect resistance as claimed in claim 1, it is characterized in that, described complex micro organism fungicide is composited by weight percent 20-30% subtilis, 15-25% bacillus amyloliquefaciens, 10-20% bacillus megaterium and 25-35% Bacillus licheniformis.
3. the composite microbiological fertilizer of disease and insect resistance as claimed in claim 1, it is characterized in that, described yeast powder includes following main component: N P and K 12-20%, medium trace element 1-3%, amino acid 3.5-6%, water-insoluble is lower than 0.5%, organic 60-75%, moisture 5%.
4. the composite microbiological fertilizer of disease and insect resistance as claimed in claim 3, it is characterized in that, described yeast powder is the pulvis of at least one in amino acid waste liquid, Gourmet Powder Factory's waste liquid, yeast factory waste liquid or sugar refinery waste liquid through concentrate drying.
5. the composite microbiological fertilizer of the disease and insect resistance as described in claim 3 or 4, is characterized in that, described yeast powder PH6.8-7.4.
6. the composite microbiological fertilizer of disease and insect resistance as claimed in claim 1, is characterized in that, described auxiliary material comprises following at least one of becoming thoroughly decomposed in material: plant residue, changing food waste, human and animal excreta, mud.
7. the preparation method of the composite microbiological fertilizer of the disease and insect resistance as described in any one of claim 1-6, is characterized in that, comprises the steps:
Yeast powder and auxiliary material are sieved according to weight part mixing, sterilizing, is mixed with the organic powder thing of moisture content below 10%;
Preparation complex micro organism fungicide;
By weight described organic powder thing and Paecilomyces lilacinus are fully mixed, then granulation, make the organic granular containing Paecilomyces lilacinus;
Make thick liquid after fully being mixed by weight with waste molasses by described complex micro organism fungicide, then by the described organic granular surface that liquid spray is prepared in above-mentioned steps, namely make the complex microorganism granulated fertilizer that microorganism is coated.
8. the preparation method of the composite microbiological fertilizer of disease and insect resistance as claimed in claim 7, is characterized in that, the described complex microorganism granulated fertilizer living bacteria count 0.2-5 hundred million/gram of preparation, npk nutrient 6-12%, moisture less than 10%.
9. the application of composite microbiological fertilizer in plant growth of disease and insect resistance prepared by the composite microbiological fertilizer of the disease and insect resistance described in any one of claim 1-6 and the preparation method described in claim 7 or 8.
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CN105777435A (en) * 2016-04-07 2016-07-20 深圳市芭田生态工程股份有限公司 Mineral substance bioorganic fertilizer and preparing method thereof
CN105948972A (en) * 2016-04-25 2016-09-21 韩德辉 Amino acid fertilizer and preparation method and application thereof
CN106008117A (en) * 2016-05-13 2016-10-12 深圳市芭田生态工程股份有限公司 Multifunctional biological organic slow-release fertilizer and preparation method thereof
CN106467434A (en) * 2016-08-31 2017-03-01 赤峰蒙鼎生物科技有限公司 A kind of NEW TYPE OF COMPOSITE microbial manure of preventing and treating vegetable insect disease
CN106748441A (en) * 2016-12-07 2017-05-31 陕西中医药大学 A kind of multiple-effect Soluble phosphorus fertilizer component for economizing on water disease-resistant and preparation method thereof
CN106810362A (en) * 2017-01-17 2017-06-09 河南漯效王生物科技股份有限公司 A kind of composite microbiological fertilizer of reduction underground root-knot nematode and preparation method thereof
CN108863649A (en) * 2018-07-03 2018-11-23 湖北鼎誉环保科技有限公司 A kind of animal dung biological organic fertilizer and preparation method thereof
CN110857258A (en) * 2018-08-06 2020-03-03 安溪全香蔬菜有限公司 Insect-attack-preventing organic environment-friendly fertilizer suitable for vegetables
EP3841881A4 (en) * 2018-08-20 2022-09-28 Agrivalle Brasil Industria e Comércio de Produtos Agrícolas Ltda Composition for biological control of phytonematodes
CN109265236A (en) * 2018-09-06 2019-01-25 广西乐土生物科技有限公司 Fertilizer containing humic acid water-soluble with insecticidal activity
EP3847891A4 (en) * 2018-09-06 2022-05-18 Agrivalle Brasil Industria e Comércio de Produtos Agrícolas Ltda Composition for coating and encrusting industrial or non-industrial seeds for biological control of phytonematode and phytopathogenic species
EP3900538A4 (en) * 2018-12-17 2022-09-14 Agrivalle Brasil Industria e Comércio de Produtos Agrícolas Ltda Composition containing microorganisms for promoting growth and reducing hydric stress in cultivated plants and use thereof
CN111072420A (en) * 2019-12-28 2020-04-28 武汉美瑞农业科技有限公司 Compound amino acid microbial agent and preparation method thereof
CN111362762A (en) * 2020-03-31 2020-07-03 南京农业大学 Granular composite bacterium bio-organic fertilizer wrapped with trichoderma spores and containing bacillus and application thereof
CN111411059A (en) * 2020-04-24 2020-07-14 黑龙江艾加生物技术有限公司 Preparation method of probiotic kitchen liquid type soil remediation agent
CN111662132A (en) * 2020-06-12 2020-09-15 上海大龙现代农业科技有限公司 Special biological organic fertilizer with weeding function for rice field and preparation method thereof
CN111995472A (en) * 2020-09-15 2020-11-27 成都云图控股股份有限公司 Disease-resistant growth-promoting type composite slow-release fertilizer and preparation method thereof
CN112250516A (en) * 2020-11-03 2021-01-22 山东和田旺生物科技有限公司 Inorganic-organic microbial compound fertilizer and preparation method thereof
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CN112980750A (en) * 2021-05-10 2021-06-18 广西壮族自治区中国科学院广西植物研究所 Compound microbial agent, compound microbial fertilizer, preparation method of compound microbial fertilizer and application of compound microbial fertilizer in paris polyphylla
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