KR20160017784A - Bacillus subtilis, Bacillus pumilus and Bacillus laterosporus strain mixture promoting plant growth and uses thereof - Google Patents

Abstract

The present invention relates to a Bacillus subtilis, Bacillus pumilus, and Bacillus laterosporus strain mixture promoting plant growth; a microorganism preparation for promoting plant growth comprising the strain mixture or a culture medium thereof as an active component; a biological fertilizer for promoting plant growth comprising the strain mixture or a culture medium thereof as active component; a method for manufacturing the biological fertilizer comprising a step of culturing the strain mixture; and a method for promoting plant growth comprising a step of soaking or spraying a plant or a seed of a plant in the strain mixture.

Description

Bacillus subtilis, Bacillus pumilus and Bacillus laterosporus strain mixture promoting plant growth and uses thereof,

The present invention relates to a mixture of Bacillus subtilis, Bacillus pertilus, and Bacillus lateosporus strains for promoting plant growth, and more particularly to a mixture of Bacillus subtilis , Bacillus subtilis , A plant growth promoting microorganism preparation comprising the mixture of Bacillus pumilus and Bacillus laterosporus strain, the above-mentioned strain mixture or a culture thereof as an active ingredient, a plant mixture containing the above-mentioned strain mixture or a culture thereof as an active ingredient, A method for producing a biocidal fertilizer comprising the step of cultivating the above-mentioned strain mixture, and a method for promoting the growth of a plant including a step of immersing or crossing the above-mentioned strain mixture in seeds of plants or plants will be.

Microorganisms are part of the recirculation as natural nutrients that continue to supply the resources needed for survival in the growth of plants. In recent years, major advanced countries have been making efforts to increase the agricultural use of microorganisms and natural substances that have secured environmental safety through the development and production of eco-friendly agricultural materials from biological organisms in preparation for environmental pollution caused by organic synthetic chemical pesticides. .

Currently, microbial agents produced and sold domestically are distributed to general farmers through inefficient materials circulated through a manufacturing process that has little or no occurrence effect due to the absence of a certain management method. It is effective to increase the yield by promoting the growth of crops by using microorganisms which are suitable for soil environment of domestic agricultural land and promoting the growth of crops (plants), and production and dissemination of environmentally friendly microorganisms ensuring safety in terms of toxicity It is a matter of urgency.

In addition to the direct effects on the crops, it is possible to improve the micro-organisms in the soil environment to improve the intelligence, thereby allowing the crops to grow more healthily. On the other hand, by imparting or enhancing the inherent solubility of the crops, It is also necessary to develop and use microorganisms for safety. In Korea, the biggest problem in the industrialization using microorganisms is the lack of a comprehensive control tower that can manage the domestic microorganism industry, the research support for industrialization is still insufficient, and the microorganism resources can not be excavated, .

Korean Patent Registration No. 0530885 discloses Pseudomonas fluorescens strain B16, a method for promoting the growth of crops using the same, and a method for controlling bacterial wilt disease. Korean Patent Registration No. 0755509 discloses a novel strain Azo Spirilum brassilens CW301, a biologic fertilizer using the same and a method for producing the same are disclosed. Korean Patent No. 0686596 discloses a new strain KLP1 for promoting plant growth and a plant growth promoting method using the same, , There has been no report on the 'mixture of Bacillus subtilis, Bacillus pertilus and Bacillus lactosporus strains and their use for promoting plant growth'.

SUMMARY OF THE INVENTION The present invention has been made in view of the above needs, and the inventors of the present invention have found that when a mixed culture of Bacillus subtilis, Bacillus pertilus, and Bacillus longusporus strains mixed in the same amount is applied to a leafy vegetable crop, In contrast, the present inventors completed the present invention by confirming that the growth of two kinds of Chinese cabbage and red mustard is markedly accelerated as compared with the untreated control.

In order to solve the above-mentioned problems, the present invention relates to a plant growth promoting Bacillus subtilis subtilis , Bacillus pumilus and Bacillus laterosporus strains.

The present invention also provides a microorganism preparation for accelerating plant growth comprising the above-mentioned strain mixture or a culture thereof as an active ingredient.

The present invention also provides a biosimilar for promoting plant growth comprising the above-mentioned strain mixture or a culture thereof as an active ingredient.

In addition, the present invention provides a method for producing a biological fertilizer comprising culturing the strain mixture.

In addition, the present invention provides a method for promoting the growth of a plant comprising the step of immersing or treating the strain of the plant with a plant or a seed of a plant.

Since the mixture of Bacillus subtilis, Bacillus pertilus and Bacillus lateosporus strain of the present invention has the characteristics of plant growth-promoting rhizobacteria (PGPR), the plant growth promotion using the strain mixture of the present invention This method can increase the growth of the crops and increase the yield of the crops, and it can be used industrially.

Fig. 1 (A) shows growth of the top and bottom portions of the Chinese cabbage by microbial treatment, and Fig. 2 (B) shows the results of comparing the growth of the Chinese cabbage by the microbial treatment.
FIG. 2 (A) shows the growth of the top and bottom portions of Chinese cabbage by microorganism treatment, and FIG. 2 (B) shows the results of comparing the growth of cabbage by the microbial treatment.
Fig. 3 (A) shows the growth of the ground and underground parts of red mustard by microbial treatment, and Fig. 3 (B) shows the results of comparing the growth of red mustard by microbial treatment.
Fig. 4 (A) shows growth of ground and underground parts of lettuce by microbial treatment, and Fig. 4 (B) shows the results of comparing the growth of lettuce by microbial treatment.
Fig. 5 (A) shows the growth of the top and bottom portions of the cheek gum by microbial preparation treatment, and Fig. 5 (B) shows the results of comparing the growth of the cheek gum by microbial preparation treatment.
1 to 5, NC is treated with distilled water, PC is treated with culture medium for microbial culture, and Treat is treated with the microorganism preparation of the present invention.

In order to accomplish the above object, the present invention relates to a plant growth promoting Bacillus subtilis subtilis , Bacillus pumilus and Bacillus laterosporus strains.

The Bacillus subtilis Subtilis , Bacillus pumilus and Bacillus laterosporus were deposited under the accession numbers KACC 12707, KACC 12712 and KACC 12713 from the National Institute of Agricultural Science, Rural Development Administration, respectively.

In the strain mixture according to one embodiment of the present invention,

The strain mixture was mixed with Bacillus subtilis subtilis), Bacillus buffer milreoseu (Bacillus pumilus), and Bacillus latte Los porous (Bacillus laterosporus) strains, but can be mixed with an equal volume (CFU / ㎖), but is not limited thereto.

The present invention also provides a microorganism preparation for accelerating plant growth comprising the above-mentioned strain mixture or a culture thereof as an active ingredient.

In the microorganism preparation for promoting plant growth according to an embodiment of the present invention, the plant may be most plants, preferably a lignocellulosic crop, and most preferably a cabbage or a red mustard. It does not.

The present invention also provides a biosimilar for promoting plant growth comprising the above-mentioned strain mixture or a culture thereof as an active ingredient. The microbial formulation according to the present invention may be prepared in the form of a liquid fertilizer, and may be added in the form of a powdered powder or may be granulated by formulating it into a powdery fertilizer. However, the formulation is not particularly limited. In other words, it can be formulated as biological fertilizer to overcome this in eco - friendly organic agriculture with limited supply of chemical fertilizer. In addition, the present invention provides a method of using the mixture of Bacillus subtilis, Bacillus subtilis and Bacillus longusporus strains as a bioproduct for promoting the growth of crops. Using the culture medium in which the strain has been cultured, it can be used as a liquid in the state of being cultivated, or it can be used by immersing or spraying seeds of crops or coating seeds.

In addition, the present invention provides a method for producing a biological fertilizer comprising culturing the strain mixture. The method for culturing the mixture of Bacillus subtilis, Bacillus pertilus, and Bacillus lateosporus strain and the method for producing the biologic fertilizer may be any method known in the art and is not particularly limited to a specific method.

In addition, the present invention provides a method for promoting the growth of a plant comprising the step of immersing or treating the strain of the plant with a plant or a seed of a plant. As a method for promoting the growth of the plant, a culture solution obtained by culturing a mixture of the Bacillus subtilis, Bacillus pertilus and Bacillus farrosporus strains and the microorganism preparation using the strain is immersed in a seed or a plant, Can be performed. In the case of the immersion method, the culture medium and the preparation can be poured into the soil around the plant or the seed can be immersed in the culture medium and the preparation.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Materials and methods

1) Manufacture of microbial cocktail

A) Selection of microorganisms for the production of microbial cocktails

The microorganism preparation to be produced in the present invention was intended to produce a product having an effect as a bio-fertilizer in leaf vegetables. Therefore, among the various microorganisms, three kinds of microorganism strains which are considered to be suitable for the cultivation of leafy vegetables are Bacillus subtilis subtilis) (KACC 12707), the Bacillus spread milreoseu (B. pumilus) (KACC 12712 ) and Bacillus Lateran Los porous (B. laterosporus) (KACC 12713 ) was produced from the pre-sale accepts the RDA National Academy of Agricultural Sciences.

B) Main characteristics of selected strains

Bacillus subtilis secretes antibiotics so that the crops are resistant to the bacterium, so that the nematode, the red pepper disease, and the Bacillus buffer milreoseu (B. pumilus) so as to raise the resistance against gray mold or the like were to also promote the healthy growth of crops with antibiotic. Here the Bacillus Lateran Los porous (B. Laterosporus) causing resistance to flooding to prevent the fatal disease that causes damage in cabbage cultivation was mixed (Table 1).

Scientific name system Guaranteed content
(CFU / ml) Culture conditions ratio Remarks Temperature
(° C) Days
(Work) badge pH Bacillus subtilis GB03 1 x 10 ⁶ 37 One NA 8 One Antibiotic secretion
Nematodes, pepper plague,
Gray Mold Bacillus pumilus AP70 1 x 10 ⁶ 37 One TSA 8 One Antibiotic action Bacillus laterosporus AP162 1 x 10 ⁶ 30 One TSA 6.8 One Immersion resistance

C) Culture of microbial cocktails

The medium for culturing the selected three strains was cultured under the conditions shown in Table 2. The incubation temperature was 34 ° C, the pH was not adjusted separately, and the agitation speed was adjusted to 250 rpm.

Chemicals One letter Content (g / L) Glucose Per pod 15 Yeast extract Yeast extract 3 Peptone Peptone 2

1st phosphate 2

Second phosphate 2 MSG Glutamate 2

Magnesium sulfate One NaCl Dry salt One

Manganese sulfate 0.02

Iron sulfate 0.02

Ammonium sulfate One

Calcium carbonate One C S L Corn dip 2 Sum 30.04

2) Analysis of effects of microbial cocktail application on crop growth

A) Materials and methods

The plant materials used in the present invention were five varieties from 4 different types of leafy vegetables such as 2 varieties of Chinese cabbage, 1 varietal of lettuce, 1 varietal varieties of Chrysanthemum sp.

B) Preparing the tray

The trays were filled with a 50-gauge trays for horticultural soil, and seeds of 3 to 5 lobes were sown and covered to a depth of about 1 cm for each crop per each area. 5 mL of water was supplied to NC (negative control), 5 mL of culture medium for culturing microorganism was added to the same amount of water for PC (positive control), and treatment was performed using the same amount 5 ml of the microbial preparation was added to the water, and the mixture was well mixed. Then, a 50-gauge tray having been seeded on the water tray was placed thereon, and water was supplied by the bottom absorption method.

As time elapsed, only the water was supplied as water, but at 1 and 2 weeks after sowing, the culture and the microbial preparation were mixed with each other at a ratio previously supplied.

On the 10th day after sowing, each plant was sown with leaving only one week. After 30 days, 20 individuals were sampled for each treatment, , Live weight at the ground level, root weight, and underground live weight. The five cultivars tested showed a germination rate of 95% or higher and were suitable for use as a test material. Most of the crops emerged around 2 days after sowing, and appeared more than 95% after 5 days and showed normal growth over time.

Example  1. Effect of microbial cocktail application on cabbage growth

Seoul cabbage was sown on a 50 - well tray and treated with microbial agent three times to compare the growth with control (NC and PC). After 30 days of sowing, the growth was examined and the difference in growth between the control and treatment zones was compared (Fig. 1). Among the trait characteristics, the plant height was 14.5 ㎝ for NC, 16.7 ㎝ for PC, 17.5 ㎝ for microbial treatment, 3 ㎝ for NC and 0.8 ㎝ for PC. Among the characteristics of the undergrowth traits, NC was 14.3 ㎝ and PC 16.9 ㎝, and microbiological treatment was 20.0 ㎝, 5.7 ㎝ longer than NC and 3.1 ㎝ longer than PC. The overall length of the plant was 28.8 cm for NC and 33.6 cm for PC. The microbial treatment was 37.5 cm, 8.7 cm longer than NC and 3.9 cm longer than PC. The fresh weight was 18.4g for NC and 20.9g for PC, and 24.0g for microbial treatment.

 The growth of Chinese cabbage (NC and PC) was compared with that of the control. After 30 days of sowing, growth was examined and the difference in growth between the control and the treatment area was compared (FIG. 2).

The number of leaves was 5.1 in NC and 6.0 in PC. The number of microbiological treatment was 6.7, which was 1.6 more than NC. Among the characteristics of the undergrowth traits, NC was 17.1 cm and PC was 18.7 cm. The microbial treatment was 23.4 cm, 6.3 cm longer than NC and 4.7 cm longer than PC. The overall length of the plant was 33.3 cm for NC and 33.6 cm for PC. The microbial treatment was 38.7 cm, 5.4 cm longer than NC and 5.1 cm longer than PC. The fresh weight was 21.1g for NC, 23.3g for PC, and 27.6g for microbial treatment.

Example  2. Fertility of microbial cocktails Mustard  Effect on growth

Red mustard seeds were sown on a 50 - gauge tray and treated with microbial agent three times to compare growth with control (NC and PC). After 30 days from sowing, growth was examined and the difference in growth between the control and the treatment area was compared (Fig. 3).

Among the trait characteristics, NC was 17.5 cm, PC was 18.2 cm, and microbial treatment was 16.9 cm, showing no statistically significant difference. This tendency was the same among leaves and living organisms. Among the characteristics of the undergrowth traits, NC and PC were 19.9 cm in length, 23.7 cm in microbiological treatment, 3.8 cm longer than NC and PC. The overall length of the plant was 23.6 cm for NC and 23.9 cm for PC. The microbial treatment was 26.7 cm, 3.3 cm longer than NC and 3.0 cm longer than PC, and the growth promoting effect was observed by microbial treatment, . The underground live weight was 23.6g for NC, 23.9g for PC, and 26.9g for microbial treatment.

Example  3. Fertility of microbial cocktails Redeemed Main  Effect on Growth of Lettuce

The main lettuce was sown on a 50 - well tray and treated with microbial agent three times to compare the growth with control (NC and PC). After 30 days of sowing, the growth was examined and the difference in growth between the control and the treatments was compared (Fig. 4).

Among the trait characteristics, NC was 17.3 ㎝, PC 17.6 ㎝, and microbial treatment 17.8 ㎝. Leaf number was 5.0 in NC and 5.6 in PC. The number of microbiological treatment was 6.6 in NC, 1.6 in NC and 1.0 in PC. Among the traits of the undergrowth traits, NC and NC were 18.8 cm and 17.6 cm, respectively, but microbial treatment was 15.8 cm, which was shorter than NC and PC but not significant. The overall length of the plant was 36.1 cm for NC and 35.2 cm for PC. The microbial treatment was 33.6 cm shorter than that of NC and PC, but there was no significant difference with fresh weight.

Example  4. Fertility of microbial cocktails Cheonggyeongae  Effect on growth

The seedlings were sown in a 50 - well tray and treated with microbial agent three times to compare the growth with control (NC and PC). After 30 days from sowing, the growth was examined and the difference in growth between the control and the treatments was compared (Fig. 5).

Among the trait characteristics, NC was 14.6 cm in length, 15.7 cm in PC, and 15.2 cm in microbial treatment. Leaf number and fresh weight were not statistically significant. Among the characteristics of the undergrowth traits, NC and PC were 14.0 ㎝ and 14.4 ㎝, respectively, but the microbial treatment was decreased to 12.4 ㎝. There was no significant difference in underground live weight. The overall length of the plant was 28.6 cm for NC and 30.1 cm for PC, but the microbial treatment decreased to 27.6 cm, but not significant. The fresh weight was 17.7g for NC and 18.3g for PC, and 15.4g for microbial treatment showed the same tendency as for the whole field.

Therefore, the microorganism preparation of the present invention promotes the growth of Chinese cabbage and red mustard, although it does not promote the growth of lettuce and cheongchyeongseong.

Claims (7)

Bacillus subtilis books that promote plant growth (Bacillus subtilis , Bacillus pumilus , and Bacillus laterosporus . The method according to claim 1, wherein the strain mixture is obtained by mixing Bacillus subtilis , Bacillus pumilus , and Bacillus laterosporus strains in an equivalent amount (CFU / ml) Strain mixture. A microorganism preparation for promoting plant growth, comprising the microorganism mixture according to claim 1 or 2 or a culture thereof as an active ingredient. 3. The plant growth promoting microorganism preparation according to claim 2, wherein the plant is Chinese cabbage or red mustard. A biological fertilizer for promoting plant growth, comprising the mixture of strains of claim 1 or 2 or a culture thereof as an active ingredient. A method for producing a biological fertilizer comprising culturing a mixture of strains of claim 1 or 2. A method for promoting the growth of a plant, comprising immersing or cultivating the mixture of strains of claim 1 or 2 in seeds of plants or plants.

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