CN116836023A - Microbial fertilizer special for tea trees and preparation method thereof - Google Patents

Microbial fertilizer special for tea trees and preparation method thereof Download PDF

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Publication number
CN116836023A
CN116836023A CN202310790984.7A CN202310790984A CN116836023A CN 116836023 A CN116836023 A CN 116836023A CN 202310790984 A CN202310790984 A CN 202310790984A CN 116836023 A CN116836023 A CN 116836023A
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parts
mixing
tea
fertilizer
hundred million
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CN116836023B (en
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任志红
孙海伟
魏传坤
吴焕焕
肖文敏
夏中华
张虹
周波
孙国栋
杨圣祥
张玲
徐慧媛
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TAIAN ACADEMY OF AGRICULTURAL SCIENCES
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/06Coniferophyta [gymnosperms], e.g. cypress
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/26Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00Disinfectants; Antimicrobial compounds or mixtures thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/40Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use

Abstract

The invention relates to the technical field of fertilizer preparation, in particular to a microbial fertilizer special for tea trees and a preparation method thereof. The invention provides a microbial fertilizer special for tea trees, which is prepared from the following components in parts by weight: 90-110 parts of spiramycin residues, 4-8 parts of composite microbial inoculum, 20-40 parts of livestock manure, 13-17 parts of soil conditioner, 6-10 parts of rapeseed meal, 6-10 parts of soybean meal, 3-7 parts of brown sugar, 2-6 parts of traditional Chinese medicine extract, 2-6 parts of gamma-polyglutamic acid and 2-6 parts of sodium carboxymethyl cellulose. The microbial fertilizer special for tea trees, which is prepared by the invention, has excellent growth promoting effect on tea trees, can obviously improve the tea yield of tea trees in spring, summer and autumn, and improve the quality of tea, and has a use effect obviously superior to that of fertilizers in the prior art.

Description

Microbial fertilizer special for tea trees and preparation method thereof
Technical Field
The invention relates to the technical field of fertilizer preparation, in particular to a microbial fertilizer special for tea trees and a preparation method thereof.
Background
The tea trees are planted on barren soil in mountain lands, and the fertilizer application can increase nutrient supply of the soil to the tea trees, so that normal physiological metabolic processes of the tea trees are maintained, and the tea trees have important significance for improving the yield and quality of the tea trees. Along with the large-area popularization of clone tea tree varieties, the chemical fertilizer consumption of tea gardens is gradually increased as part of tea agriculture pursues high yield on one surface. Excessive fertilization not only increases production cost, but also causes environmental problems such as soil acidification, non-point source pollution, greenhouse gas emission and the like. Research on searching for new fertilizer sources to replace or partially replace chemical fertilizers is increasingly important, biological bacterial fertilizers are generated, and the biological bacterial fertilizers can improve the physicochemical properties of the soil, increase the content of available nutrients in the soil and promote the absorption of the nutrients by crops when applied to the soil.
The bacterial manure provided by the prior art has the following problems. The nutrient concentration is low, the fertilizer supply is slow, so that the yield increasing effect is not obvious as that of the fertilizer, and the nutrition of crops cannot be supplied for a long time. Because the bacterial fertilizer is composed of living bacteria, direct sunlight and mixed application with pesticides are generally prohibited, and the requirements on environmental conditions such as soil temperature, humidity, pH value, organic matter content and the like are also strict.
For the barren planting environment of tea trees, the microbial colonization and propagation are more unfavorable, so how to provide a special fertilizer for tea trees, which has long fertilizer efficiency and rapid fertilizer supply, is a problem to be solved by the technicians in the field.
Disclosure of Invention
The invention aims to provide a microbial fertilizer special for tea trees and a preparation method thereof. The bacterial fertilizer prepared by the method has the advantages of quick fertilizer efficiency, long fertilizer efficiency time, excellent growth promoting effect on tea trees and capability of remarkably improving the quality and the tea yield of tea.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a microbial fertilizer special for tea trees, which is prepared from the following components in parts by weight: 90-110 parts of spiramycin residues, 4-8 parts of composite microbial inoculum, 20-40 parts of livestock manure, 13-17 parts of soil conditioner, 6-10 parts of rapeseed meal, 6-10 parts of soybean meal, 3-7 parts of brown sugar, 2-6 parts of traditional Chinese medicine extract, 2-6 parts of gamma-polyglutamic acid and 2-6 parts of sodium carboxymethyl cellulose;
the traditional Chinese medicine extract comprises the following components: purslane, chinaberry fruit, ginkgo leaf and ginkgo fruit;
the soil conditioner comprises the following components: attapulgite, vermiculite powder, kaolin and apple pomace.
Preferably, the composite microbial agent comprises: bacillus cereus, bacillus megaterium, saccharomycetes, rhodopseudomonas palustris, rhizopus oryzae and trichoderma asperellum, and the mass ratio is 8-12: 10 to 14:5 to 9:10 to 14: 4-8: 4 to 8.
Preferably, the bacillus cereus has a bacterial content of 4-8 hundred million/g; the bacteria content of the bacillus megatherium is 3-7 hundred million/g; the bacteria content of the saccharomycetes is 5-9 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 3-7 hundred million/g; the bacteria content of the rhizopus oryzae is 2-6 hundred million/g; the bacteria content of the trichoderma asperellum is 3-7 hundred million/g.
Preferably, the livestock manure comprises chicken manure, pig manure and sheep manure, and the mass ratio is 40-50: 15-25: 10 to 20.
Preferably, the mass ratio of the purslane to the chinaberry fruit to the ginkgo leaf to the ginkgo fruit is 12-16: 5 to 9:5 to 9:4 to 8.
Preferably, the mass ratio of the attapulgite, the vermiculite powder, the kaolin and the apple pomace is 9-15: 5 to 9: 7-13: 6 to 10.
The invention also provides a preparation method of the microbial fertilizer special for tea trees, which comprises the following steps:
(1) Mixing attapulgite, vermiculite powder, kaolin and apple pomace to obtain a soil conditioner;
(2) Mixing herba Portulacae, fructus Toosendan, folium Ginkgo and semen Ginkgo to obtain material 1;
(3) Mixing and decocting the material 1 with water to obtain a traditional Chinese medicine extract;
(4) Mixing spiramycin residues and a composite microbial inoculum for primary fermentation to obtain a material 2;
(5) Mixing the material 2 with livestock manure for secondary fermentation to obtain a material 3;
(6) And mixing the material 3, a soil conditioner, rapeseed meal, soybean meal, brown sugar, a traditional Chinese medicine extract, gamma-polyglutamic acid and sodium carboxymethyl cellulose to obtain the microbial fertilizer special for tea trees.
Preferably, the time of the primary fermentation in the step (4) is 50-70 h.
Preferably, the secondary fermentation in the step (5) is carried out for 5-11 days.
Preferably, in the step (3), the mass ratio of the material 1 to water is 1:3 to 5; the decoction time is 2.5-3.5 h.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention mixes and ferments the spiramycin dregs and the compound bacterial agent, which can effectively decompose the residual spiramycin in the spiramycin dregs, and mix the spiramycin dregs with the livestock manure after the fermentation is completed for further fermentation and composting treatment, and the compound bacterial agent can further release the nutrient components in the spiramycin dregs and the livestock manure, and decompose the macromolecular substances into micromolecular nutrient components, so that the fertilizer efficiency of the fertilizer is further improved.
2. The composite microbial inoculum used in the invention has reasonable addition, different probiotics can play a role in synergistically degrading spiramycin, rhizopus oryzae, trichoderma asperellum and the like can degrade cellulose and lignin in straws, and bacillus cereus, bacillus megaterium, saccharomycetes and rhodopseudomonas palustris can decompose macromolecular substances in livestock manure. Therefore, the added compound bacterial liquid has the dual functions of degrading spiramycin and decomposing macromolecular substances.
3. The attapulgite, vermiculite powder, kaolin and apple pomace added in the invention can slow down water seepage at the upper part of soil, and after being fully utilized, the attapulgite, the vermiculite powder, the kaolin and the apple pomace have large specific surface area and good physical viscosity, can block a seepage pipeline of the lower layer solid soil, reduce water seepage, effectively increase the porosity of the soil, improve the hardening of the soil and play a role in improving the soil. The traditional Chinese medicine extract added by the invention belongs to plant source pesticides, plays a role in sterilization and reduces the occurrence of tea plant diseases and insect pests.
4. The gamma-polyglutamic acid added by the invention can effectively improve the dissolution, storage, transportation and absorption of the fertilizer, prevent sulfate radical, phosphate radical, oxalate radical and metal elements from generating precipitation, enable crops to more effectively absorb phosphorus, magnesium and trace elements in soil, and promote the root system development of crops. The sodium carboxymethyl cellulose is easy to disperse in water to form transparent colloid solution, has the function of water retention, and is beneficial to the function of other components. In particular to a film formed on the surface layer of plant root hair by gamma-polyglutamic acid, which has the function of protecting the root of crops.
5. The microbial fertilizer special for tea trees, which is prepared by the invention, has excellent growth promoting effect on tea trees, can obviously improve the tea yield in spring, summer and autumn, and improves the quality of tea, and the use effect is obviously better than that of the fertilizer in the prior art.
Detailed Description
The invention provides a microbial fertilizer special for tea trees, which is prepared from the following components in parts by weight: 90-110 parts of spiramycin residues, 4-8 parts of composite microbial inoculum, 20-40 parts of livestock manure, 13-17 parts of soil conditioner, 6-10 parts of rapeseed meal, 6-10 parts of soybean meal, 3-7 parts of brown sugar, 2-6 parts of traditional Chinese medicine extract, 2-6 parts of gamma-polyglutamic acid and 2-6 parts of sodium carboxymethyl cellulose;
the preferred components are 94-106 parts of spiramycin residues, 5-7 parts of composite microbial inoculum, 24-36 parts of livestock manure, 14-16 parts of soil conditioner, 7-9 parts of rapeseed meal, 7-9 parts of bean pulp, 4-6 parts of brown sugar, 3-5 parts of traditional Chinese medicine extract, 3-5 parts of gamma-polyglutamic acid and 3-5 parts of sodium carboxymethyl cellulose;
the further preferable components are 98-102 parts of spiramycin residues, 6 parts of compound bacteria agent, 28-32 parts of livestock manure, 15 parts of soil conditioner, 8 parts of rapeseed meal, 8 parts of soybean meal, 5 parts of brown sugar, 4 parts of traditional Chinese medicine extract, 4 parts of gamma-polyglutamic acid and 4 parts of sodium carboxymethyl cellulose;
more preferably, the composition comprises 100 parts of spiramycin fungus dreg, 6 parts of a composite microbial inoculum, 30 parts of livestock manure, 15 parts of a soil conditioner, 8 parts of rapeseed meal, 8 parts of soybean meal, 5 parts of brown sugar, 4 parts of a traditional Chinese medicine extract, 4 parts of gamma-polyglutamic acid and 4 parts of sodium carboxymethyl cellulose.
The traditional Chinese medicine extract comprises the following components: purslane, chinaberry fruit, ginkgo leaf and ginkgo fruit;
the soil conditioner comprises the following components: attapulgite, vermiculite powder, kaolin and apple pomace.
In the invention, the composite microbial agent comprises: bacillus cereus, bacillus megaterium, saccharomycetes, rhodopseudomonas palustris, rhizopus oryzae and trichoderma asperellum, and the mass ratio is 8-12: 10 to 14:5 to 9:10 to 14: 4-8: 4 to 8; preferably 9 to 11:11 to 13: 6-8: 11 to 13:5 to 7:5 to 7; further preferably 10:13:7:12:6:6.
in the invention, the bacillus cereus has a bacterial content of 4-8 hundred million/g; the bacteria content of the bacillus megatherium is 3-7 hundred million/g; the bacteria content of the saccharomycetes is 5-9 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 3-7 hundred million/g; the bacteria content of the rhizopus oryzae is 2-6 hundred million/g; the bacteria content of the trichoderma asperellum is 3-7 hundred million/g;
preferably, the bacillus cereus has a bacterial content of 5-7 hundred million/g; the bacteria content of the bacillus megatherium is 4-6 hundred million/g; the bacteria content of the saccharomycetes is 6-8 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 4-6 hundred million/g; the bacteria content of the rhizopus oryzae is 3-5 hundred million/g; the bacteria content of the trichoderma asperellum is 4-6 hundred million/g;
further preferably, the bacillus cereus has a bacterial content of 6 hundred million/g; the bacteria content of the bacillus megatherium is 5 hundred million/g; the bacteria content of the saccharomycetes is 7 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 5 hundred million/g; the bacteria content of the rhizopus oryzae is 4 hundred million/g; the bacteria content of the trichoderma asperellum is 5 hundred million/g.
In the invention, the livestock manure comprises chicken manure, pig manure and sheep manure, and the mass ratio is 40-50: 15-25: 10-20 parts; preferably 42 to 48: 17-23: 12-18; more preferably 44 to 46:19 to 21:13 to 17; more preferably 45:20:15.
in the invention, the mass ratio of the purslane to the chinaberry fruit to the ginkgo leaf to the ginkgo fruit is 12-16: 5 to 9:5 to 9:4 to 8; preferably 13 to 15: 6-8: 6-8: 5 to 7; further preferred is 14:7:7:6.
in the invention, the mass ratio of the attapulgite, the vermiculite powder, the kaolin and the apple pomace is 9-15: 5 to 9: 7-13: 6-10; preferably 11 to 13: 6-8: 9 to 11: 7-9; further preferably 12:7:10:8.
the invention also provides a preparation method of the microbial fertilizer special for tea trees, which comprises the following steps:
(1) Mixing attapulgite, vermiculite powder, kaolin and apple pomace to obtain a soil conditioner;
(2) Mixing herba Portulacae, fructus Toosendan, folium Ginkgo and semen Ginkgo to obtain material 1;
(3) Mixing and decocting the material 1 with water to obtain a traditional Chinese medicine extract;
(4) Mixing spiramycin residues and a composite microbial inoculum for primary fermentation to obtain a material 2;
(5) Mixing the material 2 with livestock manure for secondary fermentation to obtain a material 3;
(6) And mixing the material 3, a soil conditioner, rapeseed meal, soybean meal, brown sugar, a traditional Chinese medicine extract, gamma-polyglutamic acid and sodium carboxymethyl cellulose to obtain the microbial fertilizer special for tea trees.
In the invention, the time of the primary fermentation in the step (4) is 50-70 h; preferably 54 to 66 hours; further preferably 58 to 62 hours; more preferably 60h.
In the invention, the time of the secondary fermentation in the step (5) is 5-11 d; preferably 7 to 9d; further preferably 8d.
In the invention, the mass ratio of the material 1 to water in the step (3) is 1:3 to 5; preferably 1:4.
In the invention, the time of the decoction in the step (3) is 2.5-3.5 hours; preferably 3h.
In the invention, the residual amount of the spiramycin in the spiramycin residues is 148.4mg/L.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A preparation method of a microbial fertilizer special for tea trees comprises the following steps:
(1) The attapulgite, vermiculite powder, kaolin and apple pomace are mixed according to the mass ratio of 9:5:7:6, mixing to obtain a soil conditioner;
(2) Chicken manure, pig manure and sheep manure are mixed according to the mass ratio of 40:15:10, mixing to obtain livestock manure;
(3) Bacillus cereus, bacillus megaterium, saccharomycetes, rhodopseudomonas palustris, rhizopus oryzae and trichoderma asperellum are mixed according to the mass ratio of 8:10:5:10:4:4, mixing to obtain a composite microbial inoculum; the bacteria content of the bacillus cereus is 4 hundred million/g; the bacteria content of the bacillus megatherium is 3 hundred million/g; the bacteria content of the saccharomycetes is 5 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 3 hundred million/g; the bacteria content of the rhizopus oryzae is 2 hundred million/g; the bacteria content of the trichoderma asperellum is 3 hundred million/g;
(4) The purslane, chinaberry fruit, ginkgo leaf and ginkgo fruit are mixed according to the mass ratio of 12:5:5:4, mixing to obtain a material 1;
(5) Mixing the material 1 with water according to the mass ratio of 1:3 mixing and decocting for 2.5h to obtain a traditional Chinese medicine extract;
(6) Mixing 90 parts of spiramycin residues and 4 parts of composite microbial inoculum, fermenting for 50 hours to obtain a material 2;
(7) Mixing and fermenting the material 2 and 20 parts of livestock manure for 5 days to obtain a material 3;
(8) And mixing the material 3, 13 parts of a soil conditioner, 6 parts of rapeseed meal, 6 parts of soybean meal, 3 parts of brown sugar, 2 parts of a traditional Chinese medicine extract, 2 parts of gamma-polyglutamic acid and 2 parts of sodium carboxymethyl cellulose to obtain the microbial fertilizer special for tea trees.
Example 2
A preparation method of a microbial fertilizer special for tea trees comprises the following steps:
(1) The attapulgite, vermiculite powder, kaolin and apple pomace are mixed according to the mass ratio of 15:9:13:10, mixing to obtain a soil conditioner;
(2) Chicken manure, pig manure and sheep manure are mixed according to the mass ratio of 50:25:20, mixing to obtain livestock manure;
(3) Bacillus cereus, bacillus megaterium, saccharomycetes, rhodopseudomonas palustris, rhizopus oryzae and trichoderma asperellum are mixed according to the mass ratio of 12:14:9:14:8:8, mixing to obtain a composite microbial inoculum; the bacteria content of the bacillus cereus is 8 hundred million/g; the bacteria content of the bacillus megatherium is 7 hundred million/g; the bacteria content of the saccharomycetes is 9 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 7 hundred million/g; the bacteria content of the rhizopus oryzae is 6 hundred million/g; the bacteria content of the trichoderma asperellum is 7 hundred million/g;
(4) The purslane, chinaberry fruit, ginkgo leaf and ginkgo fruit are mixed according to the mass ratio of 16:9:9:8, mixing to obtain a material 1;
(5) Mixing the material 1 with water according to the mass ratio of 1:5, mixing and decocting for 3.5 hours to obtain a traditional Chinese medicine extract;
(6) Mixing 110 parts of spiramycin residues and 8 parts of composite microbial inoculum, fermenting for 70 hours to obtain a material 2;
(7) Mixing and fermenting the material 2 and 40 parts of livestock manure for 11d to obtain a material 3;
(8) And mixing the material 3, 17 parts of a soil conditioner, 10 parts of rapeseed meal, 10 parts of soybean meal, 7 parts of brown sugar, 6 parts of a traditional Chinese medicine extract, 6 parts of gamma-polyglutamic acid and 6 parts of sodium carboxymethyl cellulose to obtain the microbial fertilizer special for tea trees.
Example 3
A preparation method of a microbial fertilizer special for tea trees comprises the following steps:
(1) The attapulgite, vermiculite powder, kaolin and apple pomace are mixed according to the mass ratio of 12:7:10:8, mixing to obtain a soil conditioner;
(2) Chicken manure, pig manure and sheep manure are mixed according to the mass ratio of 45:20:15, mixing to obtain livestock manure;
(3) Bacillus cereus, bacillus megaterium, saccharomycetes, rhodopseudomonas palustris, rhizopus oryzae and trichoderma asperellum are mixed according to the mass ratio of 10:12:7:12:6:6, mixing to obtain a composite microbial inoculum; the bacteria content of the bacillus cereus is 6 hundred million/g; the bacteria content of the bacillus megatherium is 5 hundred million/g; the bacteria content of the saccharomycetes is 7 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 5 hundred million/g; the bacteria content of the rhizopus oryzae is 4 hundred million/g; the bacteria content of the trichoderma asperellum is 5 hundred million/g;
(4) The purslane, chinaberry fruit, ginkgo leaf and ginkgo fruit are mixed according to the mass ratio of 14:7:7:6, mixing to obtain a material 1;
(5) Mixing the material 1 with water according to the mass ratio of 1:4, mixing and decocting for 3 hours to obtain a traditional Chinese medicine extract;
(6) Mixing and fermenting 100 parts of spiramycin residues and 6 parts of composite microbial inoculum for 60 hours to obtain a material 2;
(7) Mixing and fermenting the material 2 and 30 parts of livestock manure for 8 days to obtain a material 3;
(8) And mixing the material 3, 15 parts of a soil conditioner, 8 parts of rapeseed meal, 8 parts of soybean meal, 5 parts of brown sugar, 4 parts of a traditional Chinese medicine extract, 4 parts of gamma-polyglutamic acid and 4 parts of sodium carboxymethyl cellulose to obtain the microbial fertilizer special for tea trees.
Experimental example 1
The 10-year-old tea garden is selected as an experimental land, the planting variety is Chinese tea 108, the experimental land is divided into 30 areas, and three areas are randomly planted in each experimental group.
The fertilizer obtained by the method of example 3 was used as an experimental group;
meanwhile, a control group 1 and a control group 2 are provided to verify the influence of the soil conditioner on the tea yield and the tea quality.
Control group 1: the other methods are the same as in example 3, except that: no soil conditioner is added;
control group 2: the other methods are the same as in example 3, except that: apple pomace is not added;
meanwhile, a control group 3 and a control group 4 are provided to verify the influence of the compound microbial inoculum on the tea yield and the tea quality.
Control group 3: the other methods are the same as in example 3, except that: replacing the composite microbial inoculum with distilled water with equal quantity;
control group 4: the other methods are the same as in example 3, except that: no rhodopseudomonas palustris and rhizopus oryzae are added;
meanwhile, a control group 5 and a control group 6 are provided to verify the influence of the traditional Chinese medicine extract on the tea yield and the tea quality.
Control group 5: the other methods are the same as in example 3, except that: no ginkgo leaf or ginkgo fruit is added;
control group 6: the other methods are the same as in example 3, except that: no traditional Chinese medicine extract is added;
meanwhile, a control group 7 and a control group 8 are provided to verify the influence of gamma-polyglutamic acid and sodium carboxymethyl cellulose on the yield and quality of tea.
Control group 7: the other methods are the same as in example 3, except that: no gamma-polyglutamic acid and sodium carboxymethyl cellulose are added;
control group 8: the other methods are the same as in example 3, except that: no gamma-polyglutamic acid was added;
the prior art group: tea tree organic fertilizer (Hebei Wanbang fertilizer industry).
In the first year, the additional fertilizer is applied once in 4 months, 7 months and 10 months respectively, the application amount of the additional fertilizer is 500 kg/mu, the base fertilizer is applied in 1200 kg/mu in 12 months, the tea-picking amount in spring, summer and autumn of the second year is counted, the average value is taken, and the statistical result is shown in table 1.
TABLE 1 statistical results of spring tea yield (in kg/hm) 2 )
TABLE 2 summer tea yield statistics (units kg/hm) 2 )
Two leaves of one bud
Experimental group 5968.7
Control group1 5763.2
Control group 2 5688.4
Control group 3 4532.1
Control group 4 5317.3
Control group 5 5685.2
Control group 6 5796.4
Control group 7 5546.4
Control group 8 5487.2
Prior art group 5236.8
TABLE 3 autumn tea yield statistics (in kg/hm) 2 )
From the contents shown in table 1, the tea-picking amount of the experimental group in three tea-picking seasons is obviously improved compared with that of other control groups and the prior art groups, which shows that the microbial fertilizer special for tea trees prepared by the invention has excellent growth promotion effect on tea trees.
Experimental example 2
The experimental group, the control group and the prior art group were set as in experimental example 1, and the content of tea polyphenols and amino acids in autumn tea shoots and two leaves of each experimental group was counted and averaged, and the results are shown in Table 4.
Table 4 tea polyphenols and amino acid content questionnaires
Tea polyphenols% Amino acid% Phenol to ammonia ratio
Experimental group 21.85 4.87 4.49
Control group 1 22.45 4.52 4.97
Control group 2 22.64 4.48 5.05
Control group 3 18.65 3.82 4.88
Control group 4 24.25 4.69 5.17
Control group 5 24.87 4.79 5.19
Control group 6 23.54 4.67 5.04
Control group 7 23.38 4.62 5.06
Control group 8 21.68 4.40 4.93
Prior art group 20.54 4.17 4.93
As shown in Table 4, after the fertilizer disclosed by the invention is applied, the yield of tea leaves is improved, the amino acid content of the tea leaves is increased, the phenol-ammonia ratio is reduced, and the quality of the tea leaves is also obviously improved.
As can be seen from the data in tables 1 to 4, the soil conditioner used in the present invention further promotes the growth of tea trees because it has an improving effect on soil and improves the quality of the soil, and if the addition of the soil conditioner or a part of the components therein are discarded, the yield of tea trees and the quality of tea leaves are significantly affected.
The composite microbial inoculum provided by the invention not only has the effect of degrading antibiotics, but also can be used as beneficial bacteria, and if the composite microbial inoculum is not used or part of components in the composite microbial inoculum are discarded, the growth of tea trees can be influenced by too high antibiotic residues.
The Chinese medicinal extract has excellent sterilizing effect, and the addition of the components can reduce the infection rate of tea trees, if the components are not added, the tea trees are easy to infect pathogenic bacteria, and the growth of the tea trees is influenced. Both gamma-polyglutamic acid and sodium carboxymethyl cellulose have a synergistic effect, and if only a part of the components are added, the use effect among the components is significantly affected.
Experimental example 3
The experimental group, the control group 3 and the control group 4 are set as in the experimental example 1, after fermentation of the spiramycin dregs and the compound bacteria agent (distilled water is adopted as the control group 3) in the step (6), the residual amount of the spiramycin in the fermented product (material 2) obtained in the experimental group, the control group 3 and the control group 4 is counted, the three times of measurement are carried out, and the average value is obtained, and the result is shown in the table 5.
TABLE 5 statistical results of spiramycin residual amount (unit mg/L)
Residual amount of spiramycin
Experimental group 1.65
Control group 3 127.34
Control group 4 85.47
As is clear from the contents shown in Table 5, the method of the present invention can effectively degrade spiramycin in spiramycin residues, and if part of strains in the spiramycin residues are discarded, the degradation effect of the composite microbial inoculum on antibiotics can be significantly affected.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (10)

1. The special microbial fertilizer for tea trees is characterized by being prepared from the following components in parts by weight: 90-110 parts of spiramycin residues, 4-8 parts of composite microbial inoculum, 20-40 parts of livestock manure, 13-17 parts of soil conditioner, 6-10 parts of rapeseed meal, 6-10 parts of soybean meal, 3-7 parts of brown sugar, 2-6 parts of traditional Chinese medicine extract, 2-6 parts of gamma-polyglutamic acid and 2-6 parts of sodium carboxymethyl cellulose;
the traditional Chinese medicine extract comprises the following components: purslane, chinaberry fruit, ginkgo leaf and ginkgo fruit;
the soil conditioner comprises the following components: attapulgite, vermiculite powder, kaolin and apple pomace.
2. The fertilizer of claim 1, wherein the composite microbial agent comprises: bacillus cereus, bacillus megaterium, saccharomycetes, rhodopseudomonas palustris, rhizopus oryzae and trichoderma asperellum, and the mass ratio is 8-12: 10 to 14:5 to 9:10 to 14: 4-8: 4 to 8.
3. The fertilizer according to claim 2, wherein the bacillus cereus has a bacterial content of 4-8 v/g; the bacteria content of the bacillus megatherium is 3-7 hundred million/g; the bacteria content of the saccharomycetes is 5-9 hundred million/g; the rhodopseudomonas palustris has a bacterial content of 3-7 hundred million/g; the bacteria content of the rhizopus oryzae is 2-6 hundred million/g; the bacteria content of the trichoderma asperellum is 3-7 hundred million/g.
4. The fertilizer of claim 1, wherein the livestock manure comprises chicken manure, pig manure and sheep manure in a mass ratio of 40-50: 15-25: 10 to 20.
5. The fertilizer as claimed in claim 1, characterized in that the mass ratio of purslane, chinaberry fruit, ginkgo leaf and ginkgo fruit is 12-16: 5 to 9:5 to 9:4 to 8.
6. The fertilizer according to claim 1, wherein the mass ratio of the attapulgite, the vermiculite powder, the kaolin and the apple pomace is 9-15: 5 to 9: 7-13: 6 to 10.
7. The method for preparing the microbial fertilizer special for tea trees according to any one of claims 1 to 6, which is characterized by comprising the following steps:
(1) Mixing attapulgite, vermiculite powder, kaolin and apple pomace to obtain a soil conditioner;
(2) Mixing herba Portulacae, fructus Toosendan, folium Ginkgo and semen Ginkgo to obtain material 1;
(3) Mixing and decocting the material 1 with water to obtain a traditional Chinese medicine extract;
(4) Mixing spiramycin residues and a composite microbial inoculum for primary fermentation to obtain a material 2;
(5) Mixing the material 2 with livestock manure for secondary fermentation to obtain a material 3;
(6) And mixing the material 3, a soil conditioner, rapeseed meal, soybean meal, brown sugar, a traditional Chinese medicine extract, gamma-polyglutamic acid and sodium carboxymethyl cellulose to obtain the microbial fertilizer special for tea trees.
8. The method according to claim 7, wherein the time of the primary fermentation in the step (4) is 50 to 70 hours.
9. The method according to claim 7, wherein the secondary fermentation in step (5) is carried out for a period of 5 to 11 days.
10. The method according to claim 7, wherein the mass ratio of the material 1 to water in the step (3) is 1:3 to 5; the decoction time is 2.5-3.5 h.
CN202310790984.7A 2023-06-30 Microbial fertilizer special for tea trees and preparation method thereof Active CN116836023B (en)

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