CN110343631A - A kind of kitchen garbage bio-fermentation agents and application - Google Patents
A kind of kitchen garbage bio-fermentation agents and application Download PDFInfo
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- CN110343631A CN110343631A CN201910382753.6A CN201910382753A CN110343631A CN 110343631 A CN110343631 A CN 110343631A CN 201910382753 A CN201910382753 A CN 201910382753A CN 110343631 A CN110343631 A CN 110343631A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
A kind of kitchen garbage bio-fermentation agents are by bacillus subtilis, bacillus licheniformis, Cellumomonas flavigena, pseudomonas pseudoalcaligenes, Rhodopseudomonas palustris, Frankia, the inferior Dbaly yeast bacterium of the Chinese, four or more in Candida tropicalis is constituted and is supported on by wheat bran, sawdust, or one or both of stalk constitute carrier on and freeze-dried acquisition microbial inoculum, and then microbial inoculum and kitchen garbage be blended at 50-65 DEG C and carry out fermentation reaction, kitchen garbage minimizing 70% can be realized in 6-10 hours, and the output object content of organic matter is more than 90%.The invention has the advantages that kitchen garbage efficiently can be carried out minimizing processing by the technical method, treated, and substance can be used in soil improvement, have significant economic, society and environmental benefit.
Description
Technical field
The present invention relates to the microbial bacterial agents of processing kitchen garbage, especially a kind of kitchen garbage bio-fermentation agents and answer
With.
Background technique
Kitchen garbage is most important one kind in food refuse, including generations such as family, school, dining room and catering industries
Food process leftover bits and pieces and edible remnants.Kitchen garbage mainly by grease, water, pericarp, vegetables, rice and flour, fish, meat, bone with
And many kinds of substance such as useless tableware, plastics, paper handkerchief composition.China's kitchen garbage yield is huge, and annual output is in more than 100,000,000 tons
Rapid increase trend.Since moisture content is up to 90% in kitchen garbage, and salt content is higher.The main place of current kitchen garbage
Reason mode includes burning and filling.Due to moisture content height and the low problem of calorific value, cause the energy consumption of burning process high and easily
Uh English of pernicious gas two etc. is generated, while being also easy to that burning facility is caused to damage, causes serious production safety hidden danger.Landfill accounts for
With a large amount of soil resources, percolate is easily generated during landfill, the safety of surface water and groundwater is seriously threatened, fills out simultaneously
The process of burying leads to internal anaerobism, can generate methane, deals with explosive improperly, threatens the personal safety as well as the property safety on landfill yard and periphery.
Kitchen garbage is easily corrupt, breeds mosquitos and flies and bacterial virus, or even can breed salmonella, staphylococcus aureus, tuberculosis bar
The strong Infectious diseases bacterium such as bacterium, once diffusive transport will cause very big threat to human health in the environment.
Being directed to kitchen garbage has developed a variety of kitchen garbage processing technologies at present, however used by existing technology
Fermenting agent is mostly EM compound bacteria, however during utilizing above-mentioned microbial inoculum, fermentation time is long, is also easy to produce stench and constrains kitchen
The application of garbage aerobic processing technique.For this purpose, the present invention is tested by long-term compost, dominant bacteria is filtered out from fermentation different phase
Kind, the fermentative microflora for capableing of efficient process kitchen garbage is constructed after compounding, and effectively improves the treatment effeciency of kitchen garbage.
Summary of the invention
Present invention aims to overcome that the deficiency of existing aerobic fermentation technology, provides a kind of kitchen garbage bio-fermentation agents
And application can shorten fermentation time, improve fermentation efficiency, minimizing degree is high, and reduction is processed into compared with prior art
This.
Technical solution of the present invention:
A kind of kitchen garbage bio-fermentation agents, are made of strain component and carrier, and strain component is by following 8 kinds of microorganisms
At least four kinds of compositions, parts by weight are as follows:
15-35 parts of bacillus subtilis (Bacillus subtilis);
5-15 parts of bacillus licheniformis (Bacillus licheniformis);
15-30 parts of Cellumomonas flavigena (Cellulomonas flavigena);
10-25 parts of pseudomonas pseudoalcaligenes (Pseudomonas pesodoalcaligenes);
5-15 parts of Rhodopseudomonas palustris (Rhodopseudomonas palustris);
8-20 parts of Frankia (Frankiaceae actinomycesbovis);
5-35 parts of the inferior Dbaly yeast bacterium of the Chinese (Debaryomyces hansenii);
5-10 parts of Candida tropicalis (Candida tropicalis).
Carrier includes one or both of wheat bran, sawdust, stalk.
Bacillus subtilis, bacillus licheniformis, Cellumomonas flavigena, pseudomonas pseudoalcaligenes, marsh is red false single
Born of the same parents bacterium, Frankia in LB liquid medium, 25-40 DEG C culture 16-24 hours;
By the inferior Dbaly yeast bacterium of the Chinese, Candida tropicalis in liquid PDA culture medium, 28-42 DEG C culture 48-72 hours;
By above-mentioned bacterium solution through 10-30 minutes 9000-15000rpm centrifugal treatings, liquid is discarded supernatant, taking precipitate and carrier carry out
Mixing, and be freeze-dried in freeze dryer, to complete microbial bacterial agent preparation.
Microbial inoculum is added using primary, repeatedly intermittent addition kitchen rubbish in a kind of application of kitchen garbage bio-fermentation agents
Rubbish carries out the processing method of microorganism aerobic fermentation, and steps are as follows:
1) microbial bacterial agent is added according to the 0.1% of kitchen garbage treating capacity;
2) 30% kitchen garbage of normal treating capacity is added, and is sufficiently mixed with above-mentioned microbial inoculum, maintenance fermentation temperature is
It 50-65 DEG C, was sufficiently stirred every 30 minutes primary;
3) after the completion of through 1-2 days fermentation reactions, continue 60% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
4) after the completion of through 1-2 days fermentation reactions, continue 90% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
5) fermentation treatments of kitchen garbage can be completed through 8-16 hours, kitchen can be put into according to normal treating capacity later
Rubbish;
6) decrement 70% is completed and is realized in basic consumption in 6-10 hours of fermenting, and repetition feeds and start fermentation reaction process, circulation fortune
Row.
The kitchen garbage be the outer leaf dish leafs of the generations such as dining room, dining room, kitchen, pericarp fruit stone, leftovers it is a kind of or
It is several.
The advantages of the present invention are:
Kitchen garbage aerobic fermentation microbial inoculum is during handling kitchen garbage with fermentation efficiency is high, reduction is good, fermentation
The features such as time is short, at low cost.At the same time, the microbial inoculum is adaptable, can adapt to China's different geographical, different eating habits
Kitchen garbage processing.The content of organic matter is more than 90% in the output object of fermented reaction, and can be used as Soil improvement matrix makes
With economy with higher, environmental and social benefits.
Specific embodiment
The present invention is described in further detail by following embodiment, but the technology contents that are described of the present embodiment be it is illustrative,
Without being restrictive, protection scope of the present invention should not be limited to according to this.
Embodiment 1:
A kind of kitchen garbage bio-fermentation agents and its application, kitchen garbage bio-fermentation agents the preparation method is as follows:
1) by bacillus subtilis, bacillus licheniformis, pseudomonas pseudoalcaligenes, Rhodopseudomonas palustris, Forlan kirschner unwrapping wire
In LB liquid medium, 30 DEG C are cultivated 20 hours bacterium;By the inferior Dbaly yeast bacterium of the Chinese in liquid PDA culture medium, 28 DEG C of trainings
It supports 72 hours;
2) above-mentioned bacterium solution is discarded supernatant into liquid, taking precipitate is according to withered grass gemma respectively through 30 minutes 10000rpm centrifugal treatings
Bacillus: bacillus licheniformis: pseudomonas pseudoalcaligenes: Rhodopseudomonas palustris: Frankia: the inferior Dbaly yeast of the Chinese
Bacterium is that 18:5:20:15:20:21 is mixed with wheat bran, and is freeze-dried in freeze dryer, to obtain kitchen garbage
Bio-fermentation agents.
The kitchen garbage that the outer leaf dish leaf and leftovers in the above-mentioned fermenting agent prepared and dining room form is mixed
Fermentation is closed, fermentation step is as follows:
1) microbial bacterial agent is added according to the 0.1% of kitchen garbage treating capacity;
2) 30% kitchen garbage of normal treating capacity is added, and is sufficiently mixed with above-mentioned microbial inoculum, maintaining fermentation temperature is 50
DEG C, it was sufficiently stirred every 30 minutes primary;
3) after the completion of through 2 days fermentation reactions, continue 60% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
4) after the completion of through 2 days fermentation reactions, continue 90% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
5) fermentation treatments of kitchen garbage can be completed through 16 hours, kitchen rubbish can be put into according to normal treating capacity later
Rubbish;
6) decrement 70% is completed and is realized in basic consumption in 10 hours of fermenting, and repetition feeds and start fermentation reaction process, circulation fortune
Row.
Can be by kitchen garbage minimizing 70% using fermenting agent and method of the invention, the content of organic matter is high in output object
Up to 90%.
Embodiment 2:
A kind of kitchen garbage bio-fermentation agents and its application, kitchen garbage bio-fermentation agents the preparation method is as follows:
1) bacillus subtilis, Cellumomonas flavigena, pseudomonas pseudoalcaligenes, Rhodopseudomonas palustris are cultivated in liquid LB
In base, 32 DEG C are cultivated 22 hours;By the inferior Dbaly yeast bacterium of the Chinese, Candida tropicalis in liquid PDA culture medium, 33 DEG C of trainings
It supports 56 hours;
2) above-mentioned bacterium solution is discarded supernatant into liquid, taking precipitate is according to withered grass gemma respectively through 25 minutes 10000rpm centrifugal treatings
Bacillus: Cellumomonas flavigena: pseudomonas pseudoalcaligenes: Rhodopseudomonas palustris: the inferior Dbaly yeast bacterium of the Chinese: torrid zone vacation silk ferment
Female bacterium is that 25:15:14:10:26:10 is mixed with stalk, and is freeze-dried in freeze dryer, to obtain kitchen rubbish
Rubbish bio-fermentation agents.
The kitchen garbage that the above-mentioned fermenting agent prepared and the leftovers in dining room form is subjected to mixed fermentation, hair
Steps are as follows for ferment:
1) microbial bacterial agent is added according to the 0.1% of kitchen garbage treating capacity;
2) 30% kitchen garbage of normal treating capacity is added, and is sufficiently mixed with above-mentioned microbial inoculum, maintaining fermentation temperature is 59
DEG C, it was sufficiently stirred every 30 minutes primary;
3) after the completion of through 1.5 days fermentation reactions, continue 60% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
4) after the completion of through 1 day fermentation reaction, continue 90% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
5) fermentation treatments of kitchen garbage can be completed through 14 hours, kitchen rubbish can be put into according to normal treating capacity later
Rubbish;
6) decrement 70% is completed and is realized in basic consumption in 9 hours of fermenting, and repetition feeds and starts fermentation reaction process, circular flow.
Can be by kitchen garbage minimizing 70% using fermenting agent and method of the invention, the content of organic matter is high in output object
Up to 91%.
Embodiment 3:
A kind of kitchen garbage bio-fermentation agents and its application, kitchen garbage bio-fermentation agents the preparation method is as follows:
1) bacillus subtilis, pseudomonas pseudoalcaligenes, Rhodopseudomonas palustris, Frankia are cultivated in liquid LB
In base, 35 DEG C are cultivated 18 hours;By the inferior Dbaly yeast bacterium of the Chinese, Candida tropicalis in liquid PDA culture medium, 34 DEG C of trainings
It supports 48 hours;
2) above-mentioned bacterium solution is discarded supernatant into liquid, taking precipitate is according to bacillus subtilis respectively through 28 minutes 9000rpm centrifugal treatings
Bacterium: pseudomonas pseudoalcaligenes: Rhodopseudomonas palustris: Frankia: the inferior Dbaly yeast bacterium of the Chinese: candida tropicalis
Bacterium is that 35:17:10:20:10:8 is mixed with sawdust, and is freeze-dried in freeze dryer, to obtain kitchen garbage
Bio-fermentation agents.
The kitchen garbage that the outer leaf dish leaf and pericarp fruit stone in the above-mentioned fermenting agent prepared and kitchen form is mixed
Fermentation is closed, fermentation step is as follows:
1) microbial bacterial agent is added according to the 0.1% of kitchen garbage treating capacity;
2) 30% kitchen garbage of normal treating capacity is added, and is sufficiently mixed with above-mentioned microbial inoculum, maintaining fermentation temperature is 65
DEG C, it was sufficiently stirred every 30 minutes primary;
3) after the completion of through 1.5 days fermentation reactions, continue 60% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
4) after the completion of through 1 day fermentation reaction, continue 90% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
5) fermentation treatments of kitchen garbage can be completed through 13 hours, kitchen rubbish can be put into according to normal treating capacity later
Rubbish;
6) decrement 70% is completed and is realized in basic consumption in 8 hours of fermenting, and repetition feeds and starts fermentation reaction process, circular flow.
Can be by kitchen garbage minimizing 70% using fermenting agent and method of the invention, the content of organic matter is high in output object
Up to 92%.
Claims (3)
1. a kind of kitchen garbage bio-fermentation agents, it is characterised in that fermenting agent is made of strain component and carrier, strain group
It point is made of at least four kinds of in following 8 kinds of microorganisms, parts by weight are as follows: 15-35 parts of bacillus subtilis, lichens gemma bar
5-15 parts of bacterium, 15-30 parts of Cellumomonas flavigena, 10-25 parts of pseudomonas pseudoalcaligenes, 5-15 parts of Rhodopseudomonas palustris, not
5-35 parts, 5-10 parts of Candida tropicalis of 8-20 parts of blue kirschner actinomyces, the Chinese inferior Dbaly yeast bacterium;Carrier include wheat bran,
One or both of sawdust, stalk.Preparation method is as follows:
1) by bacillus subtilis, bacillus licheniformis, Cellumomonas flavigena, pseudomonas pseudoalcaligenes, the red false unit cell in marsh
Bacterium, Frankia in LB liquid medium, 25-40 DEG C culture 16-24 hours;
2) by the inferior Dbaly yeast bacterium of the Chinese, Candida tropicalis in liquid PDA culture medium, 28-42 DEG C of culture 48-72 is small
When;
3) by above-mentioned bacterium solution through 10-30 minutes 9000-15000rpm centrifugal treatings, discard supernatant liquid, taking precipitate and carrier into
Row mixing, and be freeze-dried in freeze dryer, to complete microbial bacterial agent preparation.
2. a kind of application of kitchen garbage bio-fermentation agents according to claim 1, it is characterised in that: be added using primary
Microbial inoculum, intermittent addition kitchen garbage carries out the processing method of microorganism aerobic fermentation repeatedly, and steps are as follows:
1) microbial bacterial agent is added according to the 0.1% of kitchen garbage treating capacity;
2) 30% kitchen garbage of normal treating capacity is added, and is sufficiently mixed with above-mentioned microbial inoculum, maintenance fermentation temperature is
It 50-65 DEG C, was sufficiently stirred every 30 minutes primary;
3) after the completion of through 1-2 days fermentation reactions, continue 60% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
4) after the completion of through 1-2 days fermentation reactions, continue 90% kitchen garbage for putting into normal treating capacity, repeat above-mentioned reaction condition;
5) fermentation treatments of kitchen garbage can be completed through 8-16 hours, kitchen can be put into according to normal treating capacity later
Rubbish;
6) decrement 70% is completed and is realized in basic consumption in 6-10 hours of fermenting, and repetition feeds and start fermentation reaction process, circulation fortune
Row.
3. a kind of application of kitchen garbage bio-fermentation agents according to claim 2, it is characterised in that: the kitchen garbage
It is one or more of for the outer leaf dish leaf of the generations such as dining room, dining room, kitchen, pericarp fruit stone, leftovers.
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110923175A (en) * | 2019-12-26 | 2020-03-27 | 江苏大道生物环境科技有限公司 | Bacillus and application thereof in reduction and resource production of organic fertilizer by kitchen waste |
CN111925968A (en) * | 2020-09-03 | 2020-11-13 | 广州希奕餐厨降解设备有限公司 | Compound microbial inoculum for kitchen waste composting and preparation method and application thereof |
CN111979158A (en) * | 2020-09-03 | 2020-11-24 | 广州希奕餐厨降解设备有限公司 | Compound microbial inoculum for food waste compost as well as preparation method and application thereof |
CN112342155A (en) * | 2020-09-28 | 2021-02-09 | 刘杰 | Kitchen waste degradation microbial agent and application thereof |
CN112934918A (en) * | 2021-02-04 | 2021-06-11 | 智旅环保科技(上海)有限公司 | Safe biological digestion method for kitchen wet garbage |
CN113789268A (en) * | 2021-08-26 | 2021-12-14 | 西南交通大学 | Composite microbial inoculum for efficiently degrading straws as well as preparation method and application thereof |
CN114736830A (en) * | 2022-05-10 | 2022-07-12 | 中南大学 | Kitchen waste treatment composite microbial inoculum, preparation method thereof and method for producing organic fertilizer by using kitchen waste treatment composite microbial inoculum |
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CN104529575A (en) * | 2015-01-16 | 2015-04-22 | 南开大学 | Method for producing high-efficiency organic fertilizer from kitchen waste |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN110923175A (en) * | 2019-12-26 | 2020-03-27 | 江苏大道生物环境科技有限公司 | Bacillus and application thereof in reduction and resource production of organic fertilizer by kitchen waste |
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CN112934918A (en) * | 2021-02-04 | 2021-06-11 | 智旅环保科技(上海)有限公司 | Safe biological digestion method for kitchen wet garbage |
CN113789268A (en) * | 2021-08-26 | 2021-12-14 | 西南交通大学 | Composite microbial inoculum for efficiently degrading straws as well as preparation method and application thereof |
CN114736830A (en) * | 2022-05-10 | 2022-07-12 | 中南大学 | Kitchen waste treatment composite microbial inoculum, preparation method thereof and method for producing organic fertilizer by using kitchen waste treatment composite microbial inoculum |
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Application publication date: 20191018 |