CN111979158A - Compound microbial inoculum for food waste compost as well as preparation method and application thereof - Google Patents
Compound microbial inoculum for food waste compost as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of environmental protection, and particularly relates to a compound microbial inoculum for food waste compost, and a preparation method and application thereof. The invention relates to a food waste compost compound microbial inoculum, which comprises the following components: the fermentation liquid of the bacillus licheniformis with a bacterium OD value of 0.3-0.9, the fermentation liquid of the paracoccus calorifaciens with a bacterium OD value of 0.3-0.9, the fermentation liquid of the streptococcus thermophilus with a bacterium OD value of 0.3-0.9 and the fermentation liquid of the pseudomonas alcaligenes with a bacterium OD value of 0.3-0.9. According to the invention, the bacillus licheniformis, the paracoccus mutilans, the streptococcus thermophilus and the pseudomonas alcaligenes are prepared into the compound microbial inoculum, so that the composting speed of the food wastes is greatly improved, the emission of malodor in the storage process is reduced, and the environment benefit is good.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and particularly relates to a compound microbial inoculum for food waste compost, and a preparation method and application thereof.
Background
The food waste has similar components with the kitchen waste, but has higher water content, nutrient components and grease components. On the one hand, the high water content characteristic of the food waste influences the normal operation of the existing household garbage transportation and transfer system, sanitary landfill system and household garbage incineration treatment system, the high oil content characteristic of the food waste also influences the normal operation of the leachate of the household garbage sanitary landfill system and the guide and discharge system of landfill gas, the existing household garbage treatment system lacks targeted solution measures for the inadaptability of the food waste characteristic, great pressure is caused to municipal household garbage treatment facilities, and the collection rate and the treatment rate of the food waste are low. On the other hand, the characteristics of high grease and high nutrient content of the food waste lead the non-standard treatment to be favorable.
At present, the food waste is degraded by adopting a composting method, so that the food waste is treated more thoroughly and resources are recycled. The composting refers to a technical method that under the conditions of certain temperature, humidity and pH, microorganisms decompose and oxidize organic solid wastes and automatically generate heat to kill pathogenic bacteria and grass seeds in raw materials to form a highly stable and humified fertilizer, so that the organic solid wastes are recycled and innoxious.
Composting is essentially a process of microbial action and therefore research on composting microbial agents is critical. At present, composting microbial inoculum applied to livestock and poultry manure (CN110184211A), composting microbial inoculum applied to straws (CN110699289A), composting microbial inoculum applied to municipal sludge (CN107032574A) and the like exist. For example, chinese patent CN106187335A discloses a composite microbial agent prepared from bacillus subtilis, bacillus coagulans or/and bacillus licheniformis for removing grease from kitchen waste.
Disclosure of Invention
The invention aims to provide a compound microbial inoculum for food waste compost as well as a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a compound microbial inoculum for food residue compost comprises the following components: the bacillus licheniformis fermentation liquor with the bacterial OD value of 0.3-0.9, the paracoccus calorigens fermentation liquor with the bacterial OD value of 0.3-0.9, the streptococcus thermophilus fermentation liquor with the bacterial OD value of 0.3-0.9 and the pseudomonas alcaligenes fermentation liquor with the bacterial OD value of 0.3-0.9. Bacillus licheniformis, Paracoccus calorigens, Streptococcus thermophilus and Pseudomonadaceae are all purchased from Guangdong province culture Collection and are numbered as GIM1.863, GIM1.487, GIM1.1808 and GIM 1.687.
The invention provides a preparation method of a complex microbial inoculum for food waste compost, which comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed solution, a colleague paracoccus seed solution, a streptococcus thermophilus seed solution and an alcaligenes pseudomonad seed solution;
A. preparing a bacillus licheniformis seed solution: inoculating bacillus licheniformis to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotation speed of 120-160 rpm to obtain the bacillus licheniformis;
B. preparation of a strain paracoccus seed solution: inoculating the paracoccus benevolens to an LB liquid culture medium, and carrying out pure culture at the temperature of 35-50 ℃ and the rotation speed of 120-160 rpm for 12-24 h to obtain the paracoccus benevolens;
C. preparation of streptococcus thermophilus seed liquid: inoculating streptococcus thermophilus to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotation speed of 120-160 rpm to obtain streptococcus thermophilus;
D. preparing an alcaligenes pseudomonad seed solution: inoculating pseudomonas alcaligenes to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm to obtain the pseudomonas alcaligenes;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium calophyllum seed liquid, the Streptococcus thermophilus seed liquid and the Pseudomonas alcaligenes seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium calophyllum fermentation liquid, a Streptococcus thermophilus fermentation liquid and a Pseudomonas alcaligenes fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the bacillus licheniformis seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the bacillus licheniformis seed liquid;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid amplification culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the paracoccus benevoleus seed liquid;
C. preparing streptococcus thermophilus fermentation liquor; transferring the streptococcus thermophilus seed liquid into a reactor filled with an LB liquid amplification culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the streptococcus thermophilus seed liquid;
D. preparing fermentation liquor of pseudomonas alcaligenes: and (3) transferring the pseudomonas alcaligenes seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min.
S3), mixing the fermentation liquor of the bacillus licheniformis, the fermentation liquor of the paracoccus mutilans, the fermentation liquor of the streptococcus thermophilus and the fermentation liquor of the pseudomonas alcaligenes in the step S2 according to the volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
Preferably, the LB liquid medium comprises the following components: 10-30 g/L of peptone, 5-8 g/L of yeast extract, 10-15 g/L of NaCl and 800-1000 mL of tap water.
Preferably, the LB liquid culture medium comprises the following components: 160-300 g/L of peptone, 80-100 g/L of yeast extract, 150-180 g/L of NaCl and 14-16L of tap water.
The invention also provides an application of the complex microbial inoculum for the food waste compost, which comprises the following steps:
(1) manually sorting the food waste by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the treated food waste;
(2) squeezing and dewatering the treated food waste obtained in the step (1) to reduce the water content of the treated food waste to 50-90% to obtain compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.01-1%, mixing and fermenting to obtain the compost.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the bacillus licheniformis, the paracoccus mutilans, the streptococcus thermophilus and the pseudomonas alcaligenes are prepared into the compound microbial inoculum, the compound microbial inoculum is used for improving the composting speed and the decomposition degree of the food waste, and simultaneously the problem of malodor in the storage process of the food waste is solved, and the method is simple and efficient and has good environmental benefits.
Detailed Description
The present invention will be described in further detail with reference to the following examples. It should not be understood that the scope of the above-described subject matter of the present invention is limited to the following examples.
Example 1
A compound microbial inoculum for food residue compost comprises the following components: a bacillus licheniformis fermentation broth with a bacterial OD value of 0.4, a colleague paracoccus fermentation broth with a bacterial OD value of 0.4, a streptococcus thermophilus fermentation broth with a bacterial OD value of 0.8 and an alcaligenes pseudomonads fermentation broth with a bacterial OD value of 0.8.
The preparation method of the complex microbial inoculum for the food waste compost comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed solution, a colleague paracoccus seed solution, a streptococcus thermophilus seed solution and an alcaligenes pseudomonad seed solution;
A. preparing a bacillus licheniformis seed solution: inoculating Bacillus licheniformis into an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 35 ℃ and the rotation speed of 120 rpm;
B. preparation of a strain paracoccus seed solution: inoculating paracoccus benomycini to an LB liquid culture medium, and carrying out pure culture at the temperature of 35 ℃ and the rotation speed of 120rpm for 12h to obtain the paracoccus benomycini extract;
C. preparation of streptococcus thermophilus seed liquid: inoculating streptococcus thermophilus into an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 35 ℃ and the rotation speed of 120 rpm;
D. preparing an alcaligenes pseudomonad seed solution: inoculating pseudomonas alcaligenes to an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 40 ℃ and the rotating speed of 120rpm to obtain the pseudomonas alcaligenes;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium calophyllum seed liquid, the Streptococcus thermophilus seed liquid and the Pseudomonas alcaligenes seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium calophyllum fermentation liquid, a Streptococcus thermophilus fermentation liquid and a Pseudomonas alcaligenes fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the Bacillus licheniformis seed liquid into a reactor filled with LB liquid propagation culture medium, and aerating at 35 deg.C and aeration intensity of 35L/min;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid amplification culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 35L/min to obtain the paracoccus benevolens seed liquid;
C. preparing streptococcus thermophilus fermentation liquor; transferring the streptococcus thermophilus seed liquid into a reactor filled with an LB liquid amplification culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 35L/min to obtain the streptococcus thermophilus seed liquid;
D. preparing fermentation liquor of pseudomonas alcaligenes: transferring the pseudomonas alcaligenes seed liquid into a reactor filled with LB liquid expanding culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 35L/min.
S3), mixing the fermentation liquor of the bacillus licheniformis, the fermentation liquor of the paracoccus mutilans, the fermentation liquor of the streptococcus thermophilus and the fermentation liquor of the pseudomonas alcaligenes in the step S2 according to the volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
Wherein, the LB liquid culture medium comprises the following components: 10g/L of peptone, 5g/L of yeast extract, 10g/L of NaCl and 1000mL of tap water; the LB liquid expanding culture medium comprises the following components: 160g/L of peptone, 80g/L of yeast extract, 150g/L of NaCl and 14L of tap water.
The application of the complex microbial inoculum for the food waste compost comprises the following steps:
(1) manually sorting the food waste by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the treated food waste;
(2) squeezing and dewatering the treated food waste obtained in the step (1) to reduce the water content of the treated food waste to 90% to obtain compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.1%, mixing and fermenting to obtain the fertilizer.
Example 2
A compound microbial inoculum for food residue compost comprises the following components: a bacillus licheniformis fermentation broth with a bacterial OD value of 0.4, a colleague paracoccus fermentation broth with a bacterial OD value of 0.6, a streptococcus thermophilus fermentation broth with a bacterial OD value of 0.7 and an alcaligenes pseudomonads fermentation broth with a bacterial OD value of 0.7.
The preparation method of the complex microbial inoculum for the food waste compost comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed solution, a colleague paracoccus seed solution, a streptococcus thermophilus seed solution and an alcaligenes pseudomonad seed solution;
A. preparing a bacillus licheniformis seed solution: inoculating Bacillus licheniformis into an LB liquid culture medium, and carrying out pure culture for 12h at 40 ℃ and a rotation speed of 120 rpm;
B. preparation of a strain paracoccus seed solution: inoculating paracoccus benomycini to an LB liquid culture medium, and carrying out pure culture at the temperature of 40 ℃ and the rotation speed of 120rpm for 12h to obtain the paracoccus benomycini extract;
C. preparation of streptococcus thermophilus seed liquid: inoculating streptococcus thermophilus into an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 40 ℃ and the rotation speed of 120 rpm;
D. preparing an alcaligenes pseudomonad seed solution: inoculating pseudomonas alcaligenes to an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 40 ℃ and the rotating speed of 120rpm to obtain the pseudomonas alcaligenes;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium calophyllum seed liquid, the Streptococcus thermophilus seed liquid and the Pseudomonas alcaligenes seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium calophyllum fermentation liquid, a Streptococcus thermophilus fermentation liquid and a Pseudomonas alcaligenes fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the Bacillus licheniformis seed liquid into a reactor filled with LB liquid propagation culture medium, and aerating at 35 deg.C and 50L/min;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid amplification culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 50L/min to obtain the paracoccus benevolens seed liquid;
C. preparing streptococcus thermophilus fermentation liquor; transferring the streptococcus thermophilus seed liquid into a reactor filled with an LB liquid amplification culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 50L/min to obtain the streptococcus thermophilus seed liquid;
D. preparing fermentation liquor of pseudomonas alcaligenes: transferring the pseudomonas alcaligenes seed liquid into a reactor filled with LB liquid expanding culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 50L/min.
S3), mixing the fermentation liquor of the bacillus licheniformis, the fermentation liquor of the paracoccus mutilans, the fermentation liquor of the streptococcus thermophilus and the fermentation liquor of the pseudomonas alcaligenes in the step S2 according to the volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
Wherein, the LB liquid culture medium comprises the following components: 10g/L of peptone, 8g/L of yeast extract, 15g/L of NaCl and 1000mL of tap water; the LB liquid expanding culture medium comprises the following components: 200g/L of peptone, 100g/L of yeast extract, 1180g/L of NaCl and 15L of tap water.
The application of the complex microbial inoculum for the food waste compost comprises the following steps:
(1) manually sorting the food waste by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the treated food waste;
(2) squeezing and dewatering the treated food waste obtained in the step (1) to reduce the water content of the treated food waste to 90% to obtain compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.1%, mixing and fermenting to obtain the fertilizer.
Example 3
A compound microbial inoculum for food residue compost comprises the following components: a bacillus licheniformis fermentation broth with a bacterial OD value of 0.8, a paracoccus lancinae fermentation broth with a bacterial OD value of 0.3, a streptococcus thermophilus fermentation broth with a bacterial OD value of 0.7, and an alcaligenes pseudomonad fermentation broth with a bacterial OD value of 0.6.
The preparation method of the complex microbial inoculum for the food waste compost comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed solution, a colleague paracoccus seed solution, a streptococcus thermophilus seed solution and an alcaligenes pseudomonad seed solution;
A. preparing a bacillus licheniformis seed solution: inoculating Bacillus licheniformis into an LB liquid culture medium, and carrying out pure culture for 17h at 40 ℃ and 140 rpm;
B. preparation of a strain paracoccus seed solution: inoculating the paracoccus benevolens to an LB liquid culture medium, and carrying out pure culture for 17h at the temperature of 40 ℃ and the rotation speed of 140rpm to obtain the paracoccus benevolens;
C. preparation of streptococcus thermophilus seed liquid: inoculating streptococcus thermophilus to an LB liquid culture medium, and carrying out pure culture for 17h at the temperature of 35 ℃ and the rotation speed of 140 rpm;
D. preparing an alcaligenes pseudomonad seed solution: inoculating the pseudomonas alcaligenes to an LB liquid culture medium, and carrying out pure culture for 17h at the temperature of 40 ℃ and the rotating speed of 140 rpm;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium calophyllum seed liquid, the Streptococcus thermophilus seed liquid and the Pseudomonas alcaligenes seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium calophyllum fermentation liquid, a Streptococcus thermophilus fermentation liquid and a Pseudomonas alcaligenes fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the Bacillus licheniformis seed liquid into a reactor filled with LB liquid propagation culture medium, and aerating at 40 deg.C and aeration intensity of 35L/min;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid amplification culture medium, and carrying out aeration at 40 ℃ and 35L/min to obtain the paracoccus benevolens seed liquid;
C. preparing streptococcus thermophilus fermentation liquor; transferring the streptococcus thermophilus seed liquid into a reactor filled with an LB liquid amplification culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 35L/min to obtain the streptococcus thermophilus seed liquid;
D. preparing fermentation liquor of pseudomonas alcaligenes: transferring the pseudomonas alcaligenes seed liquid into a reactor filled with LB liquid expanding culture medium, and carrying out aeration at 40 ℃ and 30L/min to obtain the pseudomonas alcaligenes seed liquid.
S3), mixing the fermentation liquor of the bacillus licheniformis, the fermentation liquor of the paracoccus mutilans, the fermentation liquor of the streptococcus thermophilus and the fermentation liquor of the pseudomonas alcaligenes in the step S2 according to the volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
Wherein, the LB liquid culture medium comprises the following components: peptone 20g/L, yeast extract 8g/L, NaCl 15g/L, tap water 1000 mL; the LB liquid expanding culture medium comprises the following components: 300g/L of peptone, 100g/L of yeast extract, 170g/L of NaCl and 15L of tap water.
The application of the complex microbial inoculum for the food waste compost comprises the following steps:
(1) manually sorting the food waste by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the treated food waste;
(2) squeezing and dewatering the treated food waste obtained in the step (1) to reduce the water content of the treated food waste to 90% to obtain compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.1%, mixing and fermenting to obtain the fertilizer.
Comparative example 1
Similar to example 1, the difference is that the L.carnosus fermentation broth is not added, and the total addition of the complex microbial inoculum is kept unchanged.
Comparative example 2
Similar to example 1, except that no Pseudomonas alcaligenes fermentation broth was added, the total amount of the compounded microbial inoculum was kept constant.
Experiment I, determination of maturity
1.1 subjects: examples/comparative examples/commercial complex inocula.
1.2 Experimental methods: the change degree of the organic matter content reflects the important indexes of the composting quality and the process, and the total amount of the organic matter is burned for 8 hours at 550 ℃.
TABLE 1
As can be seen from Table 1, the treatment effect of the complex microbial inoculum of the invention is better than that of the commercial complex microbial inoculum along with the increase of time.
Second experiment, odor measurement
1.1 subjects: examples/comparative examples/commercial complex inocula.
1.2 Experimental methods: gas generated in the process of degrading the food wastes is judged through smell sense, 5 persons in each group are scored, and the average value and the standard deviation are calculated.
And (4) judging the standard:
and (4) judging the standard:
90< X.ltoreq.100: without bad smell
80< X < 90: the smell is weak, and the strength and the type of the gas cannot be distinguished
70< X is less than or equal to 80: can judge which smell
60< X is less than or equal to 70: obviously smells the smell
50< X < 60: strong smell
X < 50: strong smell, unacceptable
TABLE 2
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes be made by those skilled in the art without departing from the spirit and technical spirit of the present invention, and be covered by the appended claims.
Claims (5)
1. A compound microbial inoculum for food residue compost is characterized by comprising the following components: the fermentation liquid of the bacillus licheniformis with a bacterium OD value of 0.3-0.9, the fermentation liquid of the paracoccus calorifaciens with a bacterium OD value of 0.3-0.9, the fermentation liquid of the streptococcus thermophilus with a bacterium OD value of 0.3-0.9 and the fermentation liquid of the pseudomonas alcaligenes with a bacterium OD value of 0.3-0.9.
2. The preparation method of the food waste compost microbial inoculum according to claim 1, which is characterized by comprising the following steps:
s1) respectively preparing a bacillus licheniformis seed solution, a colleague paracoccus seed solution, a streptococcus thermophilus seed solution and an alcaligenes pseudomonad seed solution;
A. preparing a bacillus licheniformis seed solution: inoculating bacillus licheniformis to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotation speed of 120-160 rpm to obtain the bacillus licheniformis;
B. preparation of a strain paracoccus seed solution: inoculating the paracoccus benevolens to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm to obtain the paracoccus benevolens;
C. preparation of streptococcus thermophilus seed liquid: inoculating streptococcus thermophilus to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm to obtain streptococcus thermophilus;
D. preparing an alcaligenes pseudomonad seed solution: inoculating pseudomonas alcaligenes to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm to obtain the pseudomonas alcaligenes;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium calophyllum seed liquid, the Streptococcus thermophilus seed liquid and the Pseudomonas alcaligenes seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium calophyllum fermentation liquid, a Streptococcus thermophilus fermentation liquid and a Pseudomonas alcaligenes fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the bacillus licheniformis seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the bacillus licheniformis seed liquid;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid culture medium for amplification, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the paracoccus benevoleus seed liquid;
C. preparing streptococcus thermophilus fermentation liquor; transferring the streptococcus thermophilus seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the streptococcus thermophilus seed liquid;
D. preparing fermentation liquor of pseudomonas alcaligenes: and (3) transferring the pseudomonas alcaligenes seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min.
S3), mixing the fermentation liquor of the bacillus licheniformis, the fermentation liquor of the paramylococcus benomyelii, the fermentation liquor of the streptococcus thermophilus and the fermentation liquor of the pseudomonas alcaligenes in the step S2 according to the volume ratio of 1: 1: 1: 1, mixing to obtain the compound microbial inoculum.
3. The preparation method of the food waste compost complex microbial inoculum according to claim 2, wherein the LB liquid culture medium comprises the following components: 10-30 g/L of peptone, 5-8 g/L of yeast extract, 10-15 g/L of NaCl and 800-1000 mL of tap water.
4. The preparation method of the complex microbial inoculum for food residue compost as claimed in claim 2, wherein the LB liquid culture medium comprises the following components: 160-300 g/L of peptone, 80-100 g/L of yeast extract, 150-180 g/L of NaCl and 14-16L of tap water.
5. The application of the complex microbial inoculum for food waste compost according to any one of claims 1 to 4, which is characterized by comprising the following steps:
(1) manually sorting the food waste by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the treated food waste;
(2) squeezing and dewatering the treated food waste obtained in the step (1) to reduce the water content of the treated food waste to 50-90% to obtain compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.01-1%, mixing and fermenting to obtain the compost.
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