CN111893079A - Compound microbial inoculum for vegetable market garbage compost as well as preparation method and application thereof - Google Patents
Compound microbial inoculum for vegetable market garbage compost as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of environmental protection, and particularly relates to a compound microbial inoculum for garbage compost in the vegetable market as well as a preparation method and application thereof. The invention relates to a garbage compost compound microbial inoculum for vegetable market, which comprises the following components: the bacterial OD value is 0.3-0.9 of the Bacillus licheniformis fermentation liquor; the bacterial OD value of the paracoccus mutilans is 0.3-0.9, and the bacterial OD value of the mosaic bacteria is 0.3-0.9; the volume ratio of the bacillus licheniformis fermentation liquor to the coprocolla plectalis fermentation liquor to the mosaic bacteria fermentation liquor is 1: 1: 1. according to the invention, the bacillus licheniformis, the paracoccus mutabilis and the mosaic bacteria are used for preparing the compound microbial inoculum for composting the garbage in the vegetable market, so that the rotten degree of the garbage in the vegetable market can be improved, the problems of transportation and storage of the garbage in the vegetable market are solved, the odor emitted by the garbage in the vegetable market is reduced, and the compound microbial inoculum has good environmental benefits.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and particularly relates to a compound microbial inoculum for garbage compost in the vegetable market as well as a preparation method and application thereof.
Background
Solid waste generated in farmer markets in daily life is called vegetable market garbage, and a lot of vegetable market garbage is generated at every moment in the life process and has great influence on the environment. The garbage in the vegetable market is usually non-toxic, and the water content of the garbage is very large, which accounts for about nine times, and the garbage in the vegetable market is very easy to decay. The garbage in the vegetable market has almost the same components as most natural organic fertilizers, the solid content of the garbage is between 8 and 19 percent, the garbage contains saccharides, hemicellulose and the like, and a certain amount of nitrogen, phosphorus, potassium and the like. The garbage collection and treatment in the vegetable market are easier because the garbage is relatively concentrated, most of the garbage is distributed around the vegetable market, the garbage is not easy to be confused with other solid garbage, the garbage is convenient to be separately recovered and treated, and the cost is relatively lower. However, most of the existing treatment modes for the garbage in the vegetable market still remain to be mixed with other household garbage for treatment, and the treatment modes not only have high cost, but also severely waste resources. Therefore, aiming at the characteristics of no toxicity, nutrient content, concentrated generation and the like of the garbage in the vegetable market, the garbage in the vegetable market is treated in a composting way, so that the garbage is treated more thoroughly, and the resource is recycled, thereby being a treatment method which has a development prospect and sustainable development at present.
The composting refers to a technical method that under the conditions of certain temperature, humidity and pH, microorganisms decompose and oxidize organic solid wastes and automatically generate heat to kill pathogenic bacteria and grass seeds in raw materials to form a highly stable and humified fertilizer, so that the organic solid wastes are recycled and innoxious. Composting is essentially a process of microbial action and therefore research on composting microbial agents is critical. At present, composting microbial inoculums applied to garbage in vegetable markets are available, for example, chinese patent CN101709006A discloses a treatment process of solid organic waste resources (food waste, household waste and garbage in vegetable markets, wastes and leftovers of slaughter houses and food factories, brewer's grains, distiller's grains, stevia rebaudiana dregs, Chinese herbal medicine dregs and the like), impurities (plastic bags, bottle caps, napkin paper, metals and the like) in the solid organic waste are removed, mixed bacteria, an enzyme preparation and auxiliary materials are added into the organic waste, the temperature is controlled by stirring and hot air, fermentation treatment is carried out by adopting a step-type temperature rise method, and the organic fertilizer is obtained by drying and crushing.
Disclosure of Invention
The invention aims to provide a compound microbial inoculum for garbage compost in the vegetable market as well as a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a compound microbial inoculum for vegetable market garbage compost is composed of: the bacterial OD value is 0.3-0.9 of the Bacillus licheniformis fermentation liquor; the bacterial OD value of the paracoccus mutilans is 0.3-0.9, and the bacterial OD value of the mosaic bacteria is 0.3-0.9; the volume ratio of the bacillus licheniformis fermentation liquor to the coprocolla plectalis fermentation liquor to the mosaic bacteria fermentation liquor is 1: 1: 1. bacillus licheniformis, Paracoccus benomyelii and Marseilles were purchased from Guangdong province culture Collection of microorganisms and numbered as GIM1.863, GIM1.487 and GIM 1.709.
The invention provides a preparation method of a complex microbial inoculum for garbage compost in the vegetable market, which comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed liquid, a paracoccus amethystoides seed liquid and a mosaic bacterium seed liquid;
preparing a bacillus licheniformis seed solution: inoculating bacillus licheniformis to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotation speed of 120-160 rpm to obtain the bacillus licheniformis;
B. preparation of a strain paracoccus seed solution: inoculating the paracoccus benevolens to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm to obtain the paracoccus benevolens;
C. preparation of a mosaic bacterium seed liquid: inoculating the mosaic bacteria to an LB liquid culture medium, and carrying out pure culture at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm for 12-24 h to obtain the mosaic bacteria;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium pleomorphus seed liquid and the mosaic bacterium seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium pleomorphus fermentation liquid and a mosaic bacterium fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the bacillus licheniformis seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the bacillus licheniformis seed liquid;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid culture medium for amplification, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the paracoccus benevoleus seed liquid;
C. preparing a mosaic bacterium fermentation liquid; and (3) transferring the mosaic bacteria seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min.
S3), mixing the Bacillus licheniformis fermentation liquor, the paracoccus mutabilis fermentation liquor and the mosaic bacteria fermentation liquor obtained in the step S2 according to the volume ratio of 1: 1: 1, mixing to obtain the compound microbial inoculum.
Preferably, the LB liquid medium comprises the following components: 10-30 g/L of peptone, 5-8 g/L of yeast extract, 10-15 g/L of sodium chloride and 800-1000 mL of tap water.
Preferably, the LB liquid culture medium comprises the following components: 160-300 g/L of peptone, 80-100 g/L, NaCl 150-180 g/L of yeast extract and 14-16L of tap water.
The invention provides an application of a kitchen waste compost compound microbial inoculum, which comprises the following steps:
(1) manually sorting the garbage in the vegetable market by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the processed garbage in the vegetable market;
(2) drying and dehydrating the treated vegetable market garbage obtained in the step (1) to reduce the water content of the treated vegetable market garbage to 50% -90% to obtain a compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.01-1%, mixing and fermenting to obtain the compost.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the bacillus licheniformis, the paracoccus mutabilis and the mosaic bacteria are used for preparing the compound microbial inoculum for composting the garbage in the vegetable market, so that the rotten degree of the garbage in the vegetable market can be improved, the problems of transportation and storage of the garbage in the vegetable market are solved, the odor emitted by the garbage in the vegetable market is reduced, and the compound microbial inoculum has good environmental benefits.
Detailed Description
The present invention will be described in further detail with reference to the following examples. It should not be understood that the scope of the above-described subject matter of the present invention is limited to the following examples.
Example 1
A compound microbial inoculum for vegetable market garbage compost comprises the following components: bacillus licheniformis fermentation liquor with a bacterial OD value of 0.3; the fermentation liquor of the paracoccus amethystoides with the bacterial OD value of 0.6 and the fermentation liquor of the mosaic bacteria with the bacterial OD value of 0.9; the volume ratio of the bacillus licheniformis fermentation liquor to the paracoccus mutilalis fermentation liquor to the mosaic bacteria fermentation liquor is 1: 1: 1.
the preparation method of the complex microbial inoculum for the vegetable market garbage compost comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed liquid, a paracoccus amethystoides seed liquid and a mosaic bacterium seed liquid;
preparing a bacillus licheniformis seed solution: inoculating Bacillus licheniformis into an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 35 ℃ and the rotation speed of 120 rpm;
B. preparation of a strain paracoccus seed solution: inoculating paracoccus benomycini to an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 35 ℃ and the rotating speed of 120rpm to obtain the paracoccus benomycini extract;
C. preparation of a mosaic bacterium seed liquid: inoculating the mosaic bacteria to an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 35-50 ℃ and the rotating speed of 120rpm to obtain the mosaic bacteria;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium pleomorphus seed liquid and the mosaic bacterium seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium pleomorphus fermentation liquid and a mosaic bacterium fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the Bacillus licheniformis seed liquid into a reactor filled with LB liquid propagation culture medium, and aerating at 35 deg.C and 30L/min;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid culture medium for expanding culture at the temperature of 35 ℃ and the aeration intensity of 30L/min to obtain the paracoccus benevolens seed liquid;
C. preparing a mosaic bacterium fermentation liquid; and (3) transferring the mosaic bacteria seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 30L/min.
S3), mixing the Bacillus licheniformis fermentation liquor, the paracoccus mutabilis fermentation liquor and the mosaic bacteria fermentation liquor obtained in the step S2 according to the volume ratio of 1: 1: 1, mixing to obtain the compound microbial inoculum.
Wherein, the LB liquid culture medium comprises the following components: 10g/L of peptone, 5g/L of yeast extract, 10g/L of sodium chloride and 800mL of tap water; the LB liquid expanding culture medium comprises the following components: 200g/L of peptone, 80g/L, NaCl150 g/150 g/L of yeast extract and 14L of tap water.
The application of the complex microbial inoculum for kitchen waste compost comprises the following steps:
(1) manually sorting the garbage in the vegetable market by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the processed garbage in the vegetable market;
(2) drying and dehydrating the treated vegetable market garbage obtained in the step (1) to reduce the water content of the treated vegetable market garbage to 90% so as to obtain a compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.1%, mixing and fermenting to obtain the fertilizer.
Example 2
A compound microbial inoculum for vegetable market garbage compost comprises the following components: bacillus licheniformis fermentation liquor with a bacterial OD value of 0.3; the fermentation liquor of the paracoccus amethystoides with the bacterial OD value of 0.7 and the fermentation liquor of the mosaic bacteria with the bacterial OD value of 0.9; the volume ratio of the bacillus licheniformis fermentation liquor to the paracoccus mutilalis fermentation liquor to the mosaic bacteria fermentation liquor is 1: 1: 1.
the preparation method of the complex microbial inoculum for the vegetable market garbage compost comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed liquid, a paracoccus amethystoides seed liquid and a mosaic bacterium seed liquid;
preparing a bacillus licheniformis seed solution: inoculating Bacillus licheniformis into an LB liquid culture medium, and carrying out pure culture for 15h at 40 ℃ and 150 rpm;
B. preparation of a strain paracoccus seed solution: inoculating paracoccus benomycini to an LB liquid culture medium, and carrying out pure culture for 15h at the temperature of 40 ℃ and the rotating speed of 150rpm to obtain the paracoccus benomycini extract;
C. preparation of a mosaic bacterium seed liquid: inoculating mosaic bacteria to an LB liquid culture medium, and carrying out pure culture for 12h at the temperature of 35 ℃ and the rotating speed of 150 rpm;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium pleomorphus seed liquid and the mosaic bacterium seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium pleomorphus fermentation liquid and a mosaic bacterium fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the Bacillus licheniformis seed liquid into a reactor filled with LB liquid propagation culture medium, and aerating at 35 deg.C and 30L/min;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid culture medium for expanding culture at the temperature of 35 ℃ and the aeration intensity of 30L/min to obtain the paracoccus benevolens seed liquid;
C. preparing a mosaic bacterium fermentation liquid; and (3) transferring the mosaic bacteria seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 35L/min.
S3), mixing the Bacillus licheniformis fermentation liquor, the paracoccus mutabilis fermentation liquor and the mosaic bacteria fermentation liquor obtained in the step S2 according to the volume ratio of 1: 1: 1, mixing to obtain the compound microbial inoculum.
Wherein, the LB liquid culture medium comprises the following components: 10g/L of peptone, 8g/L of yeast extract, 10g/L of sodium chloride and 800mL of tap water; the LB liquid expanding culture medium comprises the following components: 160g/L of peptone, 100g/L, NaCl150 g/150 g/L of yeast extract and 15L of tap water.
The application of the complex microbial inoculum for kitchen waste compost comprises the following steps:
(1) manually sorting the garbage in the vegetable market by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the processed garbage in the vegetable market;
(2) drying and dehydrating the treated vegetable market garbage obtained in the step (1) to reduce the water content of the treated vegetable market garbage to 90% so as to obtain a compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.1%, mixing and fermenting to obtain the fertilizer.
Example 3
A compound microbial inoculum for vegetable market garbage compost comprises the following components: bacillus licheniformis fermentation liquor with a bacterial OD value of 0.4; the fermentation liquor of the paracoccus amethystoides with the bacterial OD value of 0.7 and the fermentation liquor of the mosaic bacteria with the bacterial OD value of 0.8; the volume ratio of the bacillus licheniformis fermentation liquor to the paracoccus mutilalis fermentation liquor to the mosaic bacteria fermentation liquor is 1: 1: 1.
the preparation method of the complex microbial inoculum for the vegetable market garbage compost comprises the following steps:
s1) respectively preparing a bacillus licheniformis seed liquid, a paracoccus amethystoides seed liquid and a mosaic bacterium seed liquid;
preparing a bacillus licheniformis seed solution: inoculating Bacillus licheniformis into an LB liquid culture medium, and carrying out pure culture for 17h at 40 ℃ and a rotation speed of 120 rpm;
B. preparation of a strain paracoccus seed solution: inoculating paracoccus benomycini to an LB liquid culture medium, and carrying out pure culture for 15h at the temperature of 35 ℃ and the rotating speed of 130rpm to obtain the paracoccus benomycini extract;
C. preparation of a mosaic bacterium seed liquid: inoculating mosaic bacteria to an LB liquid culture medium, and carrying out pure culture for 17h at the temperature of 40 ℃ and the rotating speed of 150 rpm;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium pleomorphus seed liquid and the mosaic bacterium seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium pleomorphus fermentation liquid and a mosaic bacterium fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the Bacillus licheniformis seed liquid into a reactor filled with LB liquid propagation culture medium, and aerating at 40 deg.C with aeration intensity of 35L/min;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid culture medium for expanding culture at 40 ℃ and with the aeration intensity of 35L/min to obtain the paracoccus benevoleus seed liquid;
C. preparing a mosaic bacterium fermentation liquid; and (3) transferring the mosaic bacteria seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35 ℃, and the aeration intensity is 35L/min.
S3), mixing the Bacillus licheniformis fermentation liquor, the paracoccus mutabilis fermentation liquor and the mosaic bacteria fermentation liquor obtained in the step S2 according to the volume ratio of 1: 1: 1, mixing to obtain the compound microbial inoculum.
Wherein, the LB liquid culture medium comprises the following components: peptone 20g/L, yeast extract 8g/L, sodium chloride 10g/L and tap water 1000 mL; the LB liquid expanding culture medium comprises the following components: 200g/L of peptone, 100g/L, NaCl150g/L of yeast extract and 15L of tap water.
The application of the complex microbial inoculum for kitchen waste compost comprises the following steps:
(1) manually sorting the garbage in the vegetable market by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the processed garbage in the vegetable market;
(2) drying and dehydrating the treated vegetable market garbage obtained in the step (1) to reduce the water content of the treated vegetable market garbage to 50% -90% to obtain a compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.1%, mixing and fermenting to obtain the fertilizer.
Comparative example 1
Similar to example 1, the difference is that no mosaic bacteria fermentation broth is added, and the total addition of the complex microbial inoculum remains unchanged.
Experiment I, determination of maturity
1.1 subjects: examples/comparative examples/commercial complex inocula.
1.2 Experimental methods: the change degree of the organic matter content reflects the important indexes of the composting quality and the process, and the total amount of the organic matter is burned for 8 hours at 550 ℃.
TABLE 1
| Group of | Day 0 | 3 days | 5 days |
| Example 1 | 85.5％ | 62.3％ | 44.4％ |
| Example 2 | 85.2％ | 64.5％ | 45.9％ |
| Example 3 | 85.3％ | 65.8％ | 47.6％ |
| Comparative example 1 | 85.6％ | 70.4％ | 53.8％ |
| Commercial compound microbial inoculum | 85.3％ | 76.8％ | 72.1％ |
As can be seen from Table 1, the treatment effect of the complex microbial inoculum is better than that of the commercial complex microbial inoculum along with the increase of time.
Second experiment, odor measurement
1.1 subjects: examples/comparative examples/commercial complex inocula.
1.2 Experimental methods: and judging gas generated in the garbage degradation process in the vegetable market through smell, scoring 5 persons in each group, and calculating the average value and the standard deviation.
And (4) judging the standard:
90< X.ltoreq.100: without bad smell
80< X < 90: the smell is weak, and the strength and the type of the gas cannot be distinguished
70< X is less than or equal to 80: can judge which smell
60< X is less than or equal to 70: obviously smells the smell
50< X < 60: strong smell
X < 50: strong smell, unacceptable
TABLE 2
As can be seen from the table 2, the compound microbial inoculum of the invention can reduce the generation of stink in the process of treating garbage in the vegetable market.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (5)
1. A compound microbial inoculum for vegetable market garbage compost is characterized by comprising the following components: the bacterial OD value is 0.3-0.9 of the Bacillus licheniformis fermentation liquor; the bacterial OD value of the paracoccus mutilans is 0.3-0.9, and the bacterial OD value of the mosaic bacteria is 0.3-0.9; the volume ratio of the bacillus licheniformis fermentation liquor to the coprocolla plectalis fermentation liquor to the mosaic bacteria fermentation liquor is 1: 1: 1.
2. the preparation method of the complex microbial inoculum for the compost of garbage in the vegetable market according to claim 1, which is characterized by comprising the following steps:
s1) respectively preparing a bacillus licheniformis seed liquid, a paracoccus amethystoides seed liquid and a mosaic bacterium seed liquid;
preparing a bacillus licheniformis seed solution: inoculating bacillus licheniformis to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotation speed of 120-160 rpm to obtain the bacillus licheniformis;
B. preparation of a strain paracoccus seed solution: inoculating the paracoccus benevolens to an LB liquid culture medium, and carrying out pure culture for 12-24 h at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm to obtain the paracoccus benevolens;
C. preparation of a mosaic bacterium seed liquid: inoculating the mosaic bacteria to an LB liquid culture medium, and carrying out pure culture at the temperature of 35-50 ℃ and the rotating speed of 120-160 rpm for 12-24 h to obtain the mosaic bacteria;
s2) respectively carrying out amplification culture on the Bacillus licheniformis seed liquid, the paragonium pleomorphus seed liquid and the mosaic bacterium seed liquid obtained in the step S1 to obtain a Bacillus licheniformis fermentation liquid, a paragonium pleomorphus fermentation liquid and a mosaic bacterium fermentation liquid;
A. preparing a bacillus licheniformis fermentation liquor; transferring the bacillus licheniformis seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the bacillus licheniformis seed liquid;
B. preparing a zymotic fluid of the ameliopsis paracoccus; transferring the paracoccus benevoleus seed liquid into a reactor filled with an LB liquid culture medium for amplification, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min to obtain the paracoccus benevoleus seed liquid;
C. preparing a mosaic bacterium fermentation liquid; and (3) transferring the mosaic bacteria seed liquid into a reactor filled with an LB liquid expanding culture medium, wherein the temperature is 35-50 ℃, and the aeration intensity is 25-75L/min.
S3), mixing the Bacillus licheniformis fermentation liquor, the paracoccus mutabilis fermentation liquor and the mosaic bacteria fermentation liquor obtained in the step S2 according to the volume ratio of 1: 1: 1, mixing to obtain the compound microbial inoculum.
3. The preparation method of the complex microbial inoculum for the compost of garbage in the vegetable market according to claim 1, wherein the LB liquid culture medium comprises the following components: 10-30 g/L of peptone, 5-8 g/L of yeast extract, 10-15 g/L of sodium chloride and 800-1000 mL of tap water.
4. The preparation method of the complex microbial inoculum for the compost of garbage in the vegetable market according to claim 1, wherein the LB liquid culture medium comprises the following components: 160-300 g/L of peptone, 80-100 g/L, NaCl 150-180 g/L of yeast extract and 14-16L of tap water.
5. The application of the kitchen waste compost complex microbial inoculum according to any one of claims 1 to 4, which is characterized by comprising the following steps:
(1) manually sorting the garbage in the vegetable market by a conveyor belt to remove impurities such as disposable appliances, plastics, paper towels, metal objects and the like to obtain the processed garbage in the vegetable market;
(2) drying and dehydrating the treated vegetable market garbage obtained in the step (1) to reduce the water content of the treated vegetable market garbage to 50% -90% to obtain a compost substrate;
(3) and (3) conveying the compost substrate obtained in the step (2) to a compost box, adding a compound microbial inoculum with the mass ratio of the compost substrate being 0.01-1%, mixing and fermenting to obtain the compost.
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