CN110129222A - A kind of biological organic fertilizer fermenting agent and its application - Google Patents
A kind of biological organic fertilizer fermenting agent and its application Download PDFInfo
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- CN110129222A CN110129222A CN201910378638.1A CN201910378638A CN110129222A CN 110129222 A CN110129222 A CN 110129222A CN 201910378638 A CN201910378638 A CN 201910378638A CN 110129222 A CN110129222 A CN 110129222A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The present invention discloses a kind of biological organic fertilizer fermenting agent, including following components: photosynthetic bacteria, bacillus licheniformis, bacillus subtilis, bacillus megaterium, colloid bacillus cereus, bacillus polymyxa, white-rot fungi, trichodermaharzianum, auspicious fine 32, stench bacterium.Invention additionally discloses its application methods, comprising: (1) microbial inoculum culture: proportionally mixing strain, pours into brown sugar or molasses lysate and complex microorganism bacterium solution is made, and synthetic medium is then added, Vc, KH is added after culture 3 days2PO4, continue culture and obtain fermenting agent；(2) fermenting agent is added in matrix, water content control stores up height 40-50cm in 30%-40%, and lower than 35 DEG C fermentations of temperature obtain matrix containing microbial inoculum；Progress During High-Temperature Composting in new refuse is added to ferment up to biological organic fertilizer.Organic bio-fertilizer fermenting agent prepared by the present invention can remarkably promote rubbish it is decomposed, save compost available nutrient, not only can solve environmental pollution, but also excellent biological organic fertilizer can be produced.
Description
Technical field
The present invention relates to fermenting agent technical fields, and in particular to a kind of biological organic fertilizer fermenting agent and its application.
Background technique
With the increase of the size of population and the quickening of urbanization process, the quantity of domestic waste increasingly increases, according to not
Statistics completely, it is annual to generate nearly 1.5 hundred million tons of rubbish, and the organic matter in house refuse is difficult to be decomposed by the microorganisms in a short time
It utilizes, causes the odorant generated to cause to seriously affect to ambient air quality, endanger ecological environment.
Urban garbage disposal mainly uses organic waste there are two types of method at present, and a kind of sanitary landfills is domestic at present
It mostly handles in this way outside；Another method is that house refuse is carried out screening classification, the organic waste that will be screened out
Object carries out high temperature high-rate composting and handles rubbish, has harmless processing, the advantages such as decrement, the utilization of resources, without secondary pollution, but nature
Micro organism quantity is few in composting fermentation process, and process is unstable, and fermentation time is long, and unpleasant peculiar smell can be generated during fermentation, more
Miscellaneous bacteria, pathogen and mosquitos and flies worm's ovum are planted, germ procreation is will cause in fermentation process and mosquito breeds, the environment on periphery is caused sternly
The adverse effect of weight, and the microbial inoculum higher cost that house refuse uses, cause the cost of heap fermentation to be difficult to decrease.The prior art
In much composite bacteria agent is used to be handled for domestic rubbish disposal mode, but still there are effective flora ratio is unstable,
Cause product quality and using effect unstable, and in residents in rural community heavy metal processing and it is not in place, therefore be badly in need of
There is provided it is a kind of can stablize, efficiently, the microbial bacterial agent that domestic rubbish disposal is prepared into biological organic fertilizer of low cost.
Summary of the invention
The technical problem to be solved by the present invention is to overcome drawbacks described above, provide it is a kind of can stablize, efficiently, low cost general
Domestic rubbish disposal is prepared into the microbial bacterial agent of biological organic fertilizer.
In order to solve the above-mentioned technical problem, the technical solution of the present invention is as follows:
A kind of biological organic fertilizer fermenting agent, including following components: photosynthetic bacteria, bacillus licheniformis, bacillus subtilis,
Bacillus megaterium, colloid bacillus cereus, bacillus polymyxa, white-rot fungi, trichodermaharzianum, auspicious fine 32, stench bacterium.
Preferably, a kind of biological organic fertilizer fermenting agent, each component weight proportion are as follows: 30-60 parts of photosynthetic bacteria, lichens gemma
30-60 parts of bacillus, 30-50 parts of bacillus megaterium, 30-40 parts of colloid bacillus cereus, is glued at 30-50 parts of bacillus subtilis more
It is 30-40 parts of bacillus, 30-40 parts of white-rot fungi, 25-40 parts of trichodermaharzianum, fine 32 25-35 parts, 25-30 parts of stench bacterium auspicious.
Preferably, each component weight proportion are as follows: 40 parts of photosynthetic bacteria, 40 parts of bacillus licheniformis, 40 parts of bacillus subtilis,
40 parts of bacillus megaterium, 40 parts of colloid bacillus cereus, 40 parts of bacillus polymyxa, 40 parts of white-rot fungi, 40 parts of trichodermaharzianum,
Auspicious fine 32 30 parts, 30 parts of stench bacterium.
Preferably, effective viable bacteria content total in every gram of microbial bacterial agent is not less than 2.0 × 108CFU。
Another object of the present invention provides a kind of application method of biological organic fertilizer fermenting agent, includes the following steps:
1) microbial inoculum culture: proportionally mixing strain, then pours into brown sugar or molasses lysate mixed bacteria and makes
At complex microorganism bacterium solution, synthetic medium is then added and closed seals up for safekeeping；Complex microorganism bacterium solution is put into incubator, if
Incubator temperature is set at 28-32 DEG C, after culture 3 days, Vc, KH is added2PO4, continue culture 3-5 days, obtain fermenting agent；
(2) expand: the fermenting agent that step (1) is cultivated is added in matrix, water content is controlled in 30%-40%, heap
Depositing height is 40-50cm, and control temperature is lower than 35 DEG C, when temperature is more than 35 DEG C of timely turnings, turns over from inside to outside, guarantees inside and outside temperature
Degree is consistent；Fermentation 4-7 days packs well after temperature is stablized, and open-ended obtains matrix containing microbial inoculum；Gained matrix containing microbial inoculum is added
During High-Temperature Composting is carried out into new refuse, the water content of compost is controlled in 50%-60%；
(3) compost fermentation: feeding intake according to the input amount of the matrix containing microbial inoculum for biochemical bin stores up rubbish total amount 5%,
The 30% of the input amount of matrix containing microbial inoculum is added in first half storehouse rubbish, and the 70% of the input amount of matrix containing microbial inoculum, rubbish hair is added in later half storehouse
55-65 DEG C of ferment temperature, fermentation time 7-10 days to get biological organic fertilizer.
Preferably, in step (1) photosynthetic bacteria use culture medium include following component: distilled water 1000ml, peptone 5g,
Yeast powder 5g, MgSO4·7H2O0.5g、CaCl20.3g, the pH of the culture medium are 6.8-7.0.
Preferably, bacillus licheniformis in step (1), bacillus subtilis, bacillus megaterium, bacillus polymyxa,
The culture medium that the culture of stench bacterium uses includes following component: distilled water 1000ml, peptone 5g, NaCl5g, beef extract 3g, agar
15g, the pH of the culture medium are 7.0.
Preferably, the culture medium that colloid bacillus cereus culture uses in step (1) includes following component: distilled water
1000ml, sucrose 10g, yeast extract 0.4g, K2HPO40.5g、CaCO31g、MgSO4·7H2O0.2g、FeCl30.005g, agar 15
~20g, the pH of the culture medium are 7.0-7.2.
Preferably, the culture medium that white-rot fungi culture uses in step (1) includes following component: distilled water 1000ml, horse
Bell potato 200g leachate, KH2PO43g、MgSO4·7H2O1.5g, glucose 20g, agar 20g.
Preferably, the culture medium that trichodermaharzianum, auspicious fine 32 culture use in step (1) prepares gained by following methods: going
The potato 200g of skin, is cut into small pieces, and adds water 1000ml to boil 30 minutes elimination potato balls, filtrate is complemented to 1000ml,
Add glucose 20g, agar 15g.
Preferably, step (1) described synthetic medium includes following component: distilled water 1000ml, peptone 5g, beef extract
3g, yeast extract 4g, sucrose 5g, glucose 5g, NaCl5g, KH2PO40.2g、K2HPO40.2g、MgSO40.2g、(NH4)2SO40.5g、CaCl20.1g、FeCl30.01g, the pH of the synthetic medium are 7.2-7.4.
Preferably, the mass ratio of mixed bacteria described in step (1) and brown sugar or molasses lysate is 10:1；The mixing
The mass ratio of strain and synthetic medium is 24:1.
Preferably, the additional amount of Vc described in step (1) is the 0.05% of mixed bacteria total amount；The KH2PO4Addition
Amount is the 0.15% of mixed bacteria total amount.
Preferably, matrix described in step (2) be it is decomposed after rubbish, the additional amount of the fermenting agent is matrix total amount
5%.
Compared with prior art the beneficial effects of the present invention are:
(1) organic bio-fertilizer fermenting agent prepared by the present invention can make house refuse be rapidly achieved minimizing, it is innoxious and
The purpose of recycling, the microbial inoculum have the characteristics that fermentation calefaction is fast, maintain compost temperature height, fermentation period short, can remarkably promote
Rubbish is decomposed, saves compost available nutrient, not only can solve environmental pollution, but also can produce excellent biological organic fertilizer, therefore can
It is widely used in domestic rubbish disposal.
(2) present invention preparation uses multiple bacteria compound fermentation technique, greatly simplifies production technology, reduces microbial inoculum
Production cost, the microbial bacterial agent living bacteria count amount using the method for the present invention preparation is high, and the speed of growth of strain is fast, selected
Strain has the ability for inhibiting corrupt germ growth and breeding, effect stability.
(3) using the organic fertilizer of fermenting agent of the present invention preparation, meeting in organic fertilizer agricultural standard NY 525-2012 has
The index of correlation of machine fertilizer, can effectively increase the quantity of the soil organism and profitable strain, and activating soil promotes root system of plant pair
The absorption of nutrient improves the yield and quality of crops, is the high-grade fertilizer of production green and organic farm products.
Specific embodiment
Specific embodiments of the present invention will be further explained below.It should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but and does not constitute a limitation of the invention.In addition, invention described below
Technical characteristic involved in each embodiment can be combined with each other as long as they do not conflict with each other.
Embodiment 1
A kind of biological organic fertilizer fermenting agent, each component weight proportion are as follows: 30 parts of photosynthetic bacteria, 30 parts of bacillus licheniformis,
30 parts of bacillus subtilis, 30 parts of bacillus megaterium, 30 parts of colloid bacillus cereus, 30 parts of bacillus polymyxa, white-rot fungi
30 parts, it is 25 parts of trichodermaharzianum, fine 32 25 parts, 25 parts of stench bacterium auspicious.
A kind of application method of biological organic fertilizer fermenting agent, includes the following steps:
1) microbial inoculum culture: proportionally mixing strain, then mixed bacteria is poured into brown sugar lysate be made it is compound
Microbial inoculum, wherein the mass ratio of mixed bacteria and brown sugar lysate is 10:1；Then synthetic medium and closed envelope is added
It deposits；Complex microorganism bacterium solution is put into incubator, wherein the mass ratio of mixed bacteria and synthetic medium is 24:1；Setting training
Box temperature degree is supported at 28 DEG C, after culture 3 days, be added 0.05% Vc of mixed bacteria total amount, mixed bacteria total amount 0.15%
KH2PO4, continue culture 3-5 days, obtain fermenting agent；Wherein:
The culture medium that photosynthetic bacteria uses includes following component: distilled water 1000ml, peptone 5g, yeast powder 5g, MgSO4·
7H2O0.5g、CaCl20.3g, the pH of the culture medium are 6.8；
Bacillus licheniformis, bacillus subtilis, bacillus megaterium, bacillus polymyxa, the culture of stench bacterium use
Culture medium includes following component: distilled water 1000ml, peptone 5g, NaCl5g, beef extract 3g, agar 15g, the culture medium
PH is 7.0；
The culture medium that colloid bacillus cereus culture uses includes following component: distilled water 1000ml, sucrose 10g, yeast extract
0.4g、K2HPO40.5g、CaCO31g、MgSO4·7H2O0.2g、FeCl30.005g, agar 15g, the pH of the culture medium are
7.0；
White-rot fungi culture use culture medium include following component: distilled water 1000ml, potato 200g leachate,
KH2PO43g、MgSO4·7H2O1.5g, glucose 20g, agar 20g；
The culture medium that trichodermaharzianum, auspicious fine 32 culture use prepares gained by following methods: the potato 200g of peeling is cut
At fritter, adds water 1000ml to boil 30 minutes elimination potato balls, filtrate is complemented into 1000ml, adds glucose 20g, agar
15g；
Synthetic medium includes following component: distilled water 1000ml, peptone 5g, beef extract 3g, yeast extract 4g, sucrose
5g, glucose 5g, NaCl5g, KH2PO40.2g、K2HPO40.2g、MgSO40.2g、(NH4)2SO40.5g、CaCl20.1g、
FeCl30.01g, the pH of the synthetic medium are 7.2.
(2) expand: the fermenting agent that step (1) is cultivated is added in matrix, wherein the additional amount of fermenting agent is base
The 5% of matter total amount, 40%, stockpiling height is 40cm for water content control, and control temperature is lower than 35 DEG C, when temperature be more than 35 DEG C and
When turning, turn over from inside to outside, guarantee inside and outside temperature it is consistent；Fermentation 4-7 days packs well after temperature is stablized, and open-ended is contained
Microbial inoculum matrix；Gained matrix containing microbial inoculum is added in new refuse and carries out During High-Temperature Composting, the water content of compost is controlled 60%；
(3) compost fermentation: feeding intake according to the input amount of the matrix containing microbial inoculum for biochemical bin stores up rubbish total amount 5%,
The 30% of the input amount of matrix containing microbial inoculum is added in first half storehouse rubbish, and the 70% of the input amount of matrix containing microbial inoculum, rubbish hair is added in later half storehouse
55 DEG C of ferment temperature, fermentation time 10 days to get biological organic fertilizer.
Embodiment 2
A kind of biological organic fertilizer fermenting agent, each component weight proportion are as follows: 40 parts of photosynthetic bacteria, 40 parts of bacillus licheniformis,
40 parts of bacillus subtilis, 30 parts of bacillus megaterium, 30 parts of colloid bacillus cereus, 30 parts of bacillus polymyxa, white-rot fungi
30 parts, it is 30 parts of trichodermaharzianum, fine 32 30 parts, 25 parts of stench bacterium auspicious.
A kind of application method of biological organic fertilizer fermenting agent, includes the following steps:
1) microbial inoculum culture: proportionally mixing strain, then mixed bacteria is poured into brown sugar lysate be made it is compound
Microbial inoculum, wherein the mass ratio of mixed bacteria and brown sugar lysate is 10:1；Then synthetic medium and closed envelope is added
It deposits；Complex microorganism bacterium solution is put into incubator, wherein the mass ratio of mixed bacteria and synthetic medium is 24:1；Setting training
Box temperature degree is supported at 30 DEG C, after culture 3 days, be added 0.05% Vc of mixed bacteria total amount, mixed bacteria total amount 0.15%
KH2PO4, continue culture 3-5 days, obtain fermenting agent；Wherein:
The culture medium that photosynthetic bacteria uses includes following component: distilled water 1000ml, peptone 5g, yeast powder 5g, MgSO4·
7H2O0.5g、CaCl20.3g, the pH of the culture medium are 6.8；
Bacillus licheniformis, bacillus subtilis, bacillus megaterium, bacillus polymyxa, the culture of stench bacterium use
Culture medium includes following component: distilled water 1000ml, peptone 5g, NaCl5g, beef extract 3g, agar 15g, the culture medium
PH is 7.0；
The culture medium that colloid bacillus cereus culture uses includes following component: distilled water 1000ml, sucrose 10g, yeast extract
0.4g、K2HPO40.5g、CaCO31g、MgSO4·7H2O0.2g、FeCl30.005g, agar 16g, the pH of the culture medium are
7.0；
White-rot fungi culture use culture medium include following component: distilled water 1000ml, potato 200g leachate,
KH2PO43g、MgSO4·7H2O1.5g, glucose 20g, agar 20g；
The culture medium that trichodermaharzianum, auspicious fine 32 culture use prepares gained by following methods: the potato 200g of peeling is cut
At fritter, adds water 1000ml to boil 30 minutes elimination potato balls, filtrate is complemented into 1000ml, adds glucose 20g, agar
15g；
Synthetic medium includes following component: distilled water 1000ml, peptone 5g, beef extract 3g, yeast extract 4g, sucrose
5g, glucose 5g, NaCl5g, KH2PO40.2g、K2HPO40.2g、MgSO40.2g、(NH4)2SO40.5g、CaCl20.1g、
FeCl30.01g, the pH of the synthetic medium are 7.2.
(2) expand: the fermenting agent that step (1) is cultivated is added in matrix, wherein the additional amount of fermenting agent is base
The 5% of matter total amount, 35%, stockpiling height is 45cm for water content control, and control temperature is lower than 35 DEG C, when temperature be more than 35 DEG C and
When turning, turn over from inside to outside, guarantee inside and outside temperature it is consistent；Fermentation 4-7 days packs well after temperature is stablized, and open-ended is contained
Microbial inoculum matrix；Gained matrix containing microbial inoculum is added in new refuse and carries out During High-Temperature Composting, the water content of compost is controlled 55%；
(3) compost fermentation: feeding intake according to the input amount of the matrix containing microbial inoculum for biochemical bin stores up rubbish total amount 5%,
The 30% of the input amount of matrix containing microbial inoculum is added in first half storehouse rubbish, and the 70% of the input amount of matrix containing microbial inoculum, rubbish hair is added in later half storehouse
58 DEG C of ferment temperature, fermentation time 8 days to get biological organic fertilizer.
Embodiment 3
A kind of biological organic fertilizer fermenting agent, each component weight proportion are as follows: 40 parts of photosynthetic bacteria, 40 parts of bacillus licheniformis,
40 parts of bacillus subtilis, 40 parts of bacillus megaterium, 40 parts of colloid bacillus cereus, 40 parts of bacillus polymyxa, white-rot fungi
40 parts, it is 40 parts of trichodermaharzianum, fine 32 30 parts, 30 parts of stench bacterium auspicious.
A kind of application method of biological organic fertilizer fermenting agent, includes the following steps:
1) microbial inoculum culture: proportionally mixing strain, then mixed bacteria is poured into molasses lysate be made it is compound
Microbial inoculum, wherein the mass ratio of mixed bacteria and molasses lysate is 10:1；Then synthetic medium and closed envelope is added
It deposits；Complex microorganism bacterium solution is put into incubator, wherein the mass ratio of mixed bacteria and synthetic medium is 24:1；Setting training
Box temperature degree is supported at 30 DEG C, after culture 3 days, be added 0.05% Vc of mixed bacteria total amount, mixed bacteria total amount 0.15%
KH2PO4, continue culture 3-5 days, obtain fermenting agent；Wherein:
The culture medium that photosynthetic bacteria uses includes following component: distilled water 1000ml, peptone 5g, yeast powder 5g, MgSO4·
7H2O0.5g、CaCl20.3g, the pH of the culture medium are 6.9；
Bacillus licheniformis, bacillus subtilis, bacillus megaterium, bacillus polymyxa, the culture of stench bacterium use
Culture medium includes following component: distilled water 1000ml, peptone 5g, NaCl5g, beef extract 3g, agar 15g, the culture medium
PH is 7.0；
The culture medium that colloid bacillus cereus culture uses includes following component: distilled water 1000ml, sucrose 10g, yeast extract
0.4g、K2HPO40.5g、CaCO31g、MgSO4·7H2O0.2g、FeCl30.005g, agar 18g, the pH of the culture medium are
7.1；
White-rot fungi culture use culture medium include following component: distilled water 1000ml, potato 200g leachate,
KH2PO43g、MgSO4·7H2O1.5g, glucose 20g, agar 20g；
The culture medium that trichodermaharzianum, auspicious fine 32 culture use prepares gained by following methods: the potato 200g of peeling is cut
At fritter, adds water 1000ml to boil 30 minutes elimination potato balls, filtrate is complemented into 1000ml, adds glucose 20g, agar
15g；
Synthetic medium includes following component: distilled water 1000ml, peptone 5g, beef extract 3g, yeast extract 4g, sucrose
5g, glucose 5g, NaCl5g, KH2PO40.2g、K2HPO40.2g、MgSO40.2g、(NH4)2SO40.5g、CaCl20.1g、
FeCl30.01g, the pH of the synthetic medium are 7.3.
(2) expand: the fermenting agent that step (1) is cultivated is added in matrix, wherein the additional amount of fermenting agent is base
The 5% of matter total amount, 38%, stockpiling height is 48cm for water content control, and control temperature is lower than 35 DEG C, when temperature be more than 35 DEG C and
When turning, turn over from inside to outside, guarantee inside and outside temperature it is consistent；Fermentation 4-7 days packs well after temperature is stablized, and open-ended is contained
Microbial inoculum matrix；Gained matrix containing microbial inoculum is added in new refuse and carries out During High-Temperature Composting, the water content of compost is controlled 58%；
(3) compost fermentation: feeding intake according to the input amount of the matrix containing microbial inoculum for biochemical bin stores up rubbish total amount 5%,
The 30% of the input amount of matrix containing microbial inoculum is added in first half storehouse rubbish, and the 70% of the input amount of matrix containing microbial inoculum, rubbish hair is added in later half storehouse
Ferment temperature 60 C, fermentation time 9 days to get biological organic fertilizer.
Embodiment 4
A kind of biological organic fertilizer fermenting agent, each component weight proportion are as follows: 60 parts of photosynthetic bacteria, 60 parts of bacillus licheniformis,
50 parts of bacillus subtilis, 50 parts of bacillus megaterium, 40 parts of colloid bacillus cereus, 40 parts of bacillus polymyxa, white-rot fungi
40 parts, it is 40 parts of trichodermaharzianum, fine 32 35 parts, 30 parts of stench bacterium auspicious.
A kind of application method of biological organic fertilizer fermenting agent, includes the following steps:
1) microbial inoculum culture: proportionally mixing strain, then mixed bacteria is poured into molasses lysate be made it is compound
Microbial inoculum, wherein the mass ratio of mixed bacteria and molasses lysate is 10:1；Then synthetic medium and closed envelope is added
It deposits；Complex microorganism bacterium solution is put into incubator, wherein the mass ratio of mixed bacteria and synthetic medium is 24:1；Setting training
Box temperature degree is supported at 32 DEG C, after culture 3 days, be added 0.05% Vc of mixed bacteria total amount, mixed bacteria total amount 0.15%
KH2PO4, continue culture 3-5 days, obtain fermenting agent；Wherein:
The culture medium that photosynthetic bacteria uses includes following component: distilled water 1000ml, peptone 5g, yeast powder 5g, MgSO4·
7H2O0.5g、CaCl20.3g, the pH of the culture medium are 7.0；
Bacillus licheniformis, bacillus subtilis, bacillus megaterium, bacillus polymyxa, the culture of stench bacterium use
Culture medium includes following component: distilled water 1000ml, peptone 5g, NaCl5g, beef extract 3g, agar 15g, the culture medium
PH is 7.0；
The culture medium that colloid bacillus cereus culture uses includes following component: distilled water 1000ml, sucrose 10g, yeast extract
0.4g、K2HPO40.5g、CaCO31g、MgSO4·7H2O0.2g、FeCl30.005g, agar 20g, the pH of the culture medium are
7.2；
White-rot fungi culture use culture medium include following component: distilled water 1000ml, potato 200g leachate,
KH2PO43g、MgSO4·7H2O1.5g, glucose 20g, agar 20g；
The culture medium that trichodermaharzianum, auspicious fine 32 culture use prepares gained by following methods: the potato 200g of peeling is cut
At fritter, adds water 1000ml to boil 30 minutes elimination potato balls, filtrate is complemented into 1000ml, adds glucose 20g, agar
15g；
Synthetic medium includes following component: distilled water 1000ml, peptone 5g, beef extract 3g, yeast extract 4g, sucrose
5g, glucose 5g, NaCl5g, KH2PO40.2g、K2HPO40.2g、MgSO40.2g、(NH4)2SO40.5g、CaCl20.1g、
FeCl30.01g, the pH of the synthetic medium are 7.4.
(2) expand: the fermenting agent that step (1) is cultivated is added in matrix, wherein the additional amount of fermenting agent is base
The 5% of matter total amount, 40%, stockpiling height is 50cm for water content control, and control temperature is lower than 35 DEG C, when temperature be more than 35 DEG C and
When turning, turn over from inside to outside, guarantee inside and outside temperature it is consistent；Fermentation 4-7 days packs well after temperature is stablized, and open-ended is contained
Microbial inoculum matrix；Gained matrix containing microbial inoculum is added in new refuse and carries out During High-Temperature Composting, the water content of compost is controlled 60%；
(3) compost fermentation: feeding intake according to the input amount of the matrix containing microbial inoculum for biochemical bin stores up rubbish total amount 5%,
The 30% of the input amount of matrix containing microbial inoculum is added in first half storehouse rubbish, and the 70% of the input amount of matrix containing microbial inoculum, rubbish hair is added in later half storehouse
65 DEG C of ferment temperature, fermentation time 7 days to get biological organic fertilizer.
Referring to the index of correlation of organic fertilizer in organic fertilizer agricultural standard NY 525-2012, using hair provided by the invention
Ferment bacteria fermentation house refuse produces biological organic fertilizer, and it is as follows to obtain result for sampling and measuring after compost aerobic fermentation 7 days: micro- life
Measuring living bacteria count through colony counting method after object fermentation is 1.25 × 108Cfu/g, be much higher than in organic fertilizer >=0.2 hundred million/g
Standard；The measurement of the content of organic matter uses potassium bichromate titrimetric method, and measurement result organic matter (in terms of butt) content is 54.6%,
9.6 percentage points, pH 6.7 are higher by than organic fertilizer standard 45%, between 5.5-8.5, the measurement of excrement colibacillus group is referring to GB/
T19524.1, referring to GB/T 19524.2, roundworm egg is dead for the measurement of excrement colibacillus group≤100/g (mL) and induced worm egg death rate
Rate >=95% is died, indices have reached the nutritional requirement of organic fertilizer.
The above are present pre-ferred embodiments, it is only applicable to the principle for helping to understand the embodiment of the present invention；Meanwhile for this
The those skilled in the art in field, according to the present embodiment, there will be changes in terms of specific embodiments and scope of application, comprehensive
Upper described, the contents of this specification are not to be construed as limiting the invention.
Claims (9)
1. a kind of biological organic fertilizer fermenting agent, it is characterised in that: including following components: photosynthetic bacteria, bacillus licheniformis, withered grass
Bacillus, bacillus megaterium, colloid bacillus cereus, bacillus polymyxa, white-rot fungi, trichodermaharzianum, auspicious fine 32, stench
Bacterium.
2. biological organic fertilizer fermenting agent according to claim 1, it is characterised in that: each component weight proportion are as follows: photosynthetic
30-60 parts of bacterium, 30-60 parts of bacillus licheniformis, 30-50 parts of bacillus subtilis, 30-50 parts of bacillus megaterium, colloid bud
30-40 parts of spore bacillus, 30-40 parts of bacillus polymyxa, 30-40 parts of white-rot fungi, 25-40 parts of trichodermaharzianum, auspicious fibre 3225-35
Part, 25-30 parts of stench bacterium.
3. biological organic fertilizer fermenting agent according to claim 1, it is characterised in that: each component weight proportion are as follows: photosynthetic
It is 40 parts of bacterium, 40 parts of bacillus licheniformis, 40 parts of bacillus subtilis, 40 parts of bacillus megaterium, 40 parts of colloid bacillus cereus, more
It is viscous 40 parts of bacillus, 40 parts of white-rot fungi, 40 parts of trichodermaharzianum, fine 32 30 parts, 30 parts of stench bacterium auspicious.
4. biological organic fertilizer fermenting agent according to claim 1, it is characterised in that: total in every gram of microbial bacterial agent
Effective viable bacteria content be not less than 2.0 × 108CFU。
5. a kind of application method of biological organic fertilizer fermenting agent of any of claims 1-4, it is characterised in that: packet
Include following steps:
(1) microbial inoculum culture: proportionally mixing strain, then pours into brown sugar or molasses lysate mixed bacteria and is made again
Microbial inoculum is closed, synthetic medium is then added and closed is sealed up for safekeeping；Complex microorganism bacterium solution is put into incubator, setting training
Box temperature degree is supported at 28-32 DEG C, after culture 3 days, Vc, KH is added2PO4, continue culture 3-5 days, obtain fermenting agent；
(2) expand: the fermenting agent that step (1) is cultivated is added in matrix, in 30%-40%, stockpiling is high for water content control
Degree is 40-50cm, and control temperature is lower than 35 DEG C, when temperature is more than 35 DEG C of timely turnings, turns over from inside to outside, guarantee inside and outside temperature one
It causes；Fermentation 4-7 days packs well after temperature is stablized, and open-ended obtains matrix containing microbial inoculum；Gained matrix containing microbial inoculum is added to newly
During High-Temperature Composting is carried out in fresh food garbage, the water content of compost is controlled in 50%-60%；
(3) compost fermentation: feeding intake according to the input amount of the matrix containing microbial inoculum for biochemical bin stores up rubbish total amount 5%, first half
The 30% of the input amount of matrix containing microbial inoculum is added in storehouse rubbish, and the 70% of the input amount of matrix containing microbial inoculum, rubbish fermentation temperature is added in later half storehouse
55-65 DEG C of degree, fermentation time 7-10 days to get biological organic fertilizer.
6. the application method of biological organic fertilizer fermenting agent according to claim 5, it is characterised in that: step (1) is described
Synthetic medium includes following component: distilled water 1000ml, peptone 5g, beef extract 3g, yeast extract 4g, sucrose 5g, glucose
5g、NaCl5g、KH2PO40.2g、K2HPO40.2g、MgSO40.2g、(NH4)2SO40.5g、CaCl20.1g、FeCl30.01g, institute
The pH for stating synthetic medium is 7.2-7.4.
7. the application method of biological organic fertilizer fermenting agent according to claim 5, it is characterised in that: institute in step (1)
The mass ratio for stating mixed bacteria and brown sugar or molasses lysate is 10:1；The mass ratio of the mixed bacteria and synthetic medium is
24:1。
8. the application method of biological organic fertilizer fermenting agent according to claim 5, it is characterised in that: institute in step (1)
The additional amount for stating Vc is the 0.05% of mixed bacteria total amount；The KH2PO4Additional amount be mixed bacteria total amount 0.15%.
9. the application method of biological organic fertilizer fermenting agent according to claim 5, it is characterised in that: institute in step (2)
State matrix be it is decomposed after rubbish, the additional amount of the fermenting agent is the 5% of matrix total amount.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111876359A (en) * | 2020-08-04 | 2020-11-03 | 榆林沙漠王生物科技有限公司 | Compound microbial inoculum for recycling vegetable residues and preparation method and application thereof |
| CN111893079A (en) * | 2020-09-03 | 2020-11-06 | 广州希奕餐厨降解设备有限公司 | Compound microbial inoculum for vegetable market garbage compost as well as preparation method and application thereof |
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2019
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111876359A (en) * | 2020-08-04 | 2020-11-03 | 榆林沙漠王生物科技有限公司 | Compound microbial inoculum for recycling vegetable residues and preparation method and application thereof |
| CN111893079A (en) * | 2020-09-03 | 2020-11-06 | 广州希奕餐厨降解设备有限公司 | Compound microbial inoculum for vegetable market garbage compost as well as preparation method and application thereof |
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