CN113480345A - Bio-organic fertilizer based on municipal biogas residues and livestock and poultry manure and preparation method thereof - Google Patents
Bio-organic fertilizer based on municipal biogas residues and livestock and poultry manure and preparation method thereof Download PDFInfo
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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Abstract
The invention discloses a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure and a preparation method thereof, belonging to the technical field of biological organic fertilizers, and the biological organic fertilizer comprises a basic fertilizer, a fermentation microbial inoculum and an effective microbial inoculum; the base fertilizer comprises, by weight, 45-55 parts of municipal biogas residues, 40-60 parts of livestock and poultry manure, 1-5 parts of a conditioner, 0.4-0.6 part of a heavy metal passivator and 0.03-0.04 part of antagonistic pathogenic bacteria. The biological organic fertilizer disclosed by the invention realizes harmless and resource utilization of municipal biogas residues and livestock and poultry manure, can effectively improve the soil microenvironment, promotes the growth of crops and can effectively resist soil-borne diseases.
Description
Technical Field
The invention relates to the technical field of biological organic fertilizers, in particular to a biological organic fertilizer based on municipal biogas residues and livestock and poultry cost arrangement and a preparation method thereof.
Background
In recent years, the economic society of China is rapidly developed, the urbanization process is accelerated, the discharge amount of municipal sewage in cities and towns of China is 670 billion t in 2019, the discharge amount is further increased to 700 billion t in 2020, and the numerical value is predicted to be greatly increased in 2021. Corresponding to the increase of the municipal sludge yield in China, the municipal sludge yield reaches 4000 million t (the water content is 80%) in 2016, the total yield increases in the following years, and 6000 million t is broken through in 2020. The problem that the sludge accumulation is too large and secondary pollution to the environment is possibly caused is a great challenge in the field of environmental protection.
Municipal sludge has complex components, high water content, easy decay and odor, possible deposition of toxic substances such as polychlorinated biphenyl, heavy metals and the like, more parasites and pathogenic microorganisms, and damage to underground water and soil quality if the toxic substances enter the environment due to improper disposal, thus threatening life health. However, the municipal sludge is rich in resources such as organic matters, N, P, K and beneficial metal elements, and if the resources can be reasonably converted, the municipal sludge can provide assistance for sustainable agricultural development.
At present, the domestic and foreign treatment method of municipal sludge mainly comprises sanitary landfill, incineration, energy source preparation, material preparation or compost treatment. The method has the advantages of low investment of landfill operation, quick response, easy secondary pollution of water and soil environment around the landfill site and poor environmental protection. The sludge incineration reduction effect is obvious, but SO is easily generated in the incineration process2、H2S, dioxin and other toxic gases can pollute the air.
In order to reduce the accumulation of municipal sludge, avoid the damage of toxic substances to the quality of underground water and soil and realize reasonable conversion and utilization of beneficial resources contained in the municipal sludge, the invention provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure and a preparation method thereof.
Disclosure of Invention
In order to solve the defects in the prior art and aim at resource utilization of biogas residues of a sewage treatment plant and harmless treatment of livestock and poultry manure, the invention provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure and a preparation method thereof.
The invention relates to a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure and a preparation method thereof, which are realized by the following technical scheme:
the invention provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which is characterized by comprising a base fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
45-55 parts of municipal biogas residues, 40-60 parts of livestock and poultry manure, 1-5 parts of a conditioner, 0.4-0.6 part of a heavy metal passivator and 0.03-0.04 part of antagonistic pathogenic bacteria.
Furthermore, the dosage of the fermentation inoculum is 1.5-5 wt% of the base fertilizer, and the effective viable count (cfu) of the fermentation inoculum is more than or equal to 2 hundred million/g; the dosage of the effective microbial inoculum is 1.5-2.5 wt% of the base fertilizer, and the effective viable count (cfu) of the effective microbial inoculum is more than or equal to 0.20 hundred million/g.
Further, the fermentation inoculum is one or more of saccharomyces cerevisiae, acetobacter pasteurianus AS1.41, bacillus subtilis ATCC 6633, actinomycetes 710, lactobacillus DJ8A and trichoderma reesei JC 40360.
Further, the fermentation inoculum is prepared by mixing saccharomyces cerevisiae, acetobacter pasteurianus AS1.41, bacillus subtilis ATCC 6633, actinomycetes 710, lactobacillus DJ8A and trichoderma reesei JC40360 according to a bacterial liquid volume ratio of 3:3:3:1:0.7:0.7: 0.5.
Further, the efficacy microbial inoculum is one or more of bacillus subtilis ATCC 6633, bacillus licheniformis W10, bacillus laterosporus strain YSC3, bacillus megaterium L2, bacillus amyloliquefaciens LX1, trichoderma harzianum T-22 and paecilomyces lilacinus IPC.
Further, the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633, bacillus licheniformis W10, bacillus laterosporus strain YSC3, bacillus megaterium L2, bacillus amyloliquefaciens LX1, trichoderma harzianum T-22 and paecilomyces lilacinus IPC according to the volume ratio of the bacterial liquid of 1:1:1:1:1:1: 1.
Further, the conditioner is one or more of sawdust, peanut vine, peanut shell powder, rice straw, straws, mushroom dregs, traditional Chinese medicine dregs, tobacco powder and fruit peel and vegetable leaves.
Further, the heavy metal passivator is one or more of fly ash, lime and turfy soil.
Further, the antagonistic pathogen is tea saponin.
Further, the livestock and poultry manure is one or more of cow manure and chicken manure; the water content of the cow dung is 45-55 wt%, and the water content of the chicken manure is 65-75 wt%.
Further, the municipal biogas residues are biogas residues obtained by high-dry anaerobic fermentation of municipal sludge to produce biogas.
The second purpose of the invention is to provide a preparation method of the biological organic fertilizer, which comprises the following steps:
weighing each component of a base fertilizer, adding a zymophyte agent according to 1.5-5 wt% of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack form, wherein the stack lifting size is 0.55m wide at the upper part, 2m wide at the lower part and 1.2-1.5 m high;
step two, after the first mixed material is stacked and arranged, covering a composite polymer film (the moisture permeability of which is more than or equal to 8000 g/m) on the top of the first mixed material224h, air permeability ≥ 2L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 10-20 days in an environment with the temperature not more than 65 ℃ and the oxygen concentration of 8% -18% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 7-14 days to reduce the water content of the base fertilizer to 20-30 wt% of the base fertilizer, so as to obtain a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum, and uniformly mixing to obtain the biological organic fertilizer.
Compared with the prior art, the invention has the following beneficial effects:
the production of the biological organic fertilizer selects biogas residues generated after high-dry anaerobic fermentation of a municipal sewage treatment plant for producing biogas as a main raw material, simultaneously takes the surrounding livestock and poultry breeding manure as a secondary utilizable resource for aerobic composting, adds a certain amount of organic fertilizer fermentation microbial inoculum, converts biodegradable organic matters in the raw material into stable humus by utilizing microbial catabolism, and effectively kills parasites, pathogenic bacteria and weed seeds in the biogas residues and the livestock and poultry manure at continuous high temperature in the fermentation process, thereby achieving the purposes of harmless and resource utilization of the biogas residues and the livestock and poultry manure.
The organic fertilizer is compounded with a certain amount of compound high-efficiency microbial effect microbial inoculum, so that the prepared bio-organic fertilizer product not only contains higher effective viable bacteria, but also has the advantages that the functional strains can effectively improve the soil microenvironment and improve the capability of crops in absorbing nutrient components, and antibiotics, antibacterial proteins and active polypeptides secreted by various biocontrol bacteria can promote the growth of the crops on one hand and effectively resist soil-borne diseases on the other hand.
The auxiliary material conditioner is used for adjusting the carbon-nitrogen ratio of compost materials to be 25-30: 1 properly, the selection range is combined with local agricultural waste resources, sawdust, peanut seedlings, peanut shell powder, rice straws, mushroom residues, traditional Chinese medicine residues, tobacco powder, fruit peel, vegetable leaves and the like can be selected, the fly ash is used as a heavy metal passivator, and the aim of adding tea saponin is to antagonize pathogenic bacteria. The addition of the liquid fermentation strain can improve the fermentation effect and speed, and the fermentation speed is positively correlated with the addition of the microbial inoculum. The effective bacteria agent is added into the organic fertilizer, so that the effective viable count (cfu) of the product is more than or equal to 0.20 hundred million/g, and various biocontrol strains can improve the soil environment, improve the stress resistance and nutrient absorption rate of crops and further improve the yield and quality of the crops.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below.
The municipal biogas residues in the following embodiments of the invention are municipal sludge of a certain municipal sewage treatment plant subjected to anaerobic fermentation by a dry-type anaerobic reactor to produce biogas, so as to obtain anaerobic fermentation products, and the anaerobic fermentation products are discharged in vacuum and then subjected to solid-liquid separation, so that the obtained solid is the municipal biogas residues used in the following embodiments. The high dry anaerobic fermentation of the present invention is carried out by the prior art, which is known to those skilled in the art and therefore will not be described herein.
Example 1
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
45 parts of municipal biogas residues, 55 parts of livestock and poultry manure, 5 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
Wherein the livestock and poultry manure is specifically 35 parts of cow manure (with the water content of 50 wt%) and 20 parts of chicken manure (with the water content of 70 wt%).
The conditioner is specifically 2.5 parts of thoroughly crushed sawdust and 2.5 parts of champignon mushroom dregs.
The heavy metal passivator is 0.6 part of fly ash.
The antagonistic pathogenic bacteria is specifically 0.04 part of tea saponin.
The dosage of the zymophyte agent is 3 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2 hundred million/g; and the fermentation bacteria agent is obtained by mixing saccharomyces cerevisiae bacteria liquid, acetobacter pasteurianus AS1.41 bacteria liquid, bacillus subtilis ATCC 6633 bacteria liquid, actinomycetes 710 bacteria liquid, lactobacillus DJ8A bacteria liquid and trichoderma reesei JC40360 bacteria liquid according to the volume ratio of 3:3:1:0.7:0.7: 0.5.
The dosage of the effective microbial inoculum is 2wt per thousand of the total weight of the base fertilizer, the effective viable count (cfu) of the effective microbial inoculum is 0.20 hundred million/g, and the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633 liquid, bacillus licheniformis W10 liquid, bacillus laterosporus strain YSC3 liquid, bacillus megaterium L2 liquid, bacillus amyloliquefaciens LX1 liquid, trichoderma harzianum T-22 liquid and paecilomyces lilacinus IPC liquid according to the volume ratio of the bacterial liquids of 1:1:1:1:1:1: 1.
Example 2
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
50 parts of municipal biogas residues, 55 parts of livestock and poultry manure, 5 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
Wherein the livestock and poultry manure is specifically 35 parts of cow manure (with the water content of 50 wt%) and 20 parts of chicken manure (with the water content of 70 wt%).
The conditioner is specifically 2.5 parts of thoroughly crushed sawdust and 2.5 parts of champignon mushroom dregs.
The heavy metal passivator is 0.6 part of fly ash.
The antagonistic pathogenic bacteria is specifically 0.04 part of tea saponin.
The dosage of the zymophyte agent is 3 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2.01 hundred million/g; and the fermentation bacteria agent is obtained by mixing saccharomyces cerevisiae bacteria liquid, acetobacter pasteurianus AS1.41 bacteria liquid, bacillus subtilis ATCC 6633 bacteria liquid, actinomycetes 710 bacteria liquid, lactobacillus DJ8A bacteria liquid and trichoderma reesei JC40360 bacteria liquid according to the volume ratio of 3:3:1:0.7:0.7: 0.5.
The dosage of the effective microbial inoculum is 2wt per mill of the total weight of the base fertilizer, and the effective viable count (cfu) of the effective microbial inoculum is 0.20 hundred million/g; the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633 liquid, bacillus licheniformis W10 liquid, bacillus laterosporus strain YSC3 liquid, bacillus megaterium L2 liquid, bacillus amyloliquefaciens LX1 liquid, trichoderma harzianum T-22 liquid and paecilomyces lilacinus IPC liquid according to the volume ratio of the bacterial liquids of 1:1:1:1:1:1: 1.
Example 3
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
55 parts of municipal biogas residues, 55 parts of livestock and poultry manure, 5 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
Wherein the livestock and poultry manure is specifically 25 parts of cow manure (with the water content of 50 wt%) and 20 parts of chicken manure (with the water content of 70 wt%).
The conditioner is specifically 2.5 parts of thoroughly crushed sawdust and 2.5 parts of champignon mushroom dregs.
The heavy metal passivator is 0.6 part of fly ash.
The antagonistic pathogenic bacteria is specifically 0.04 part of tea saponin.
The dosage of the zymophyte agent is 3 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2.02 hundred million/g; and the fermentation bacteria agent is obtained by mixing saccharomyces cerevisiae bacteria liquid, acetobacter pasteurianus AS1.41 bacteria liquid, bacillus subtilis ATCC 6633 bacteria liquid, actinomycetes 710 bacteria liquid, lactobacillus DJ8A bacteria liquid and trichoderma reesei JC40360 bacteria liquid according to the volume ratio of 3:3:1:0.7:0.7: 0.5.
The dosage of the effective microbial inoculum is 2wt per thousand of the total weight of the base fertilizer, the effective viable count (cfu) of the effective microbial inoculum is 0.20 hundred million/g, and the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633 liquid, bacillus licheniformis W10 liquid, bacillus laterosporus strain YSC3 liquid, bacillus megaterium L2 liquid, bacillus amyloliquefaciens LX1 liquid, trichoderma harzianum T-22 liquid and paecilomyces lilacinus IPC liquid according to the volume ratio of the bacterial liquids of 1:1:1:1:1:1: 1.
Example 4
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
45 parts of municipal biogas residues, 55 parts of livestock and poultry manure, 5 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
Wherein the livestock and poultry manure is specifically 35 parts of cow manure (with the water content of 50 wt%) and 20 parts of chicken manure (with the water content of 70 wt%).
The conditioner is specifically 2.5 parts of thoroughly crushed sawdust and 2.5 parts of champignon mushroom dregs.
The heavy metal passivator is concretely 0.6 part of turfy soil.
The antagonistic pathogenic bacteria is specifically 0.04 part of tea saponin.
The dosage of the zymophyte agent is 3 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2 hundred million/g; and the fermentation bacteria agent is obtained by mixing saccharomyces cerevisiae bacteria liquid, acetobacter pasteurianus AS1.41 bacteria liquid, bacillus subtilis ATCC 6633 bacteria liquid, actinomycetes 710 bacteria liquid, lactobacillus DJ8A bacteria liquid and trichoderma reesei JC40360 bacteria liquid according to the volume ratio of 3:3:1:0.7:0.7: 0.5.
The dosage of the effective microbial inoculum is 2wt per thousand of the total weight of the base fertilizer, the effective viable count (cfu) of the effective microbial inoculum is 0.20 hundred million/g, and the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633 liquid, bacillus licheniformis W10 liquid, bacillus laterosporus strain YSC3 liquid, bacillus megaterium L2 liquid, bacillus amyloliquefaciens LX1 liquid, trichoderma harzianum T-22 liquid and paecilomyces lilacinus IPC liquid according to the volume ratio of the bacterial liquids of 1:1:1:1:1:1: 1.
Example 5
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
45 parts of municipal biogas residues, 40 parts of livestock and poultry manure, 5 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
Wherein the livestock and poultry manure is specifically 20 parts of cow manure (with the water content of 50 wt%) and 20 parts of chicken manure (with the water content of 70 wt%).
The conditioner is specifically 2.5 parts of thoroughly crushed peanut shell powder and 2.5 parts of straws.
The heavy metal passivator is lime 0.6 part.
The antagonistic pathogenic bacteria is specifically 0.04 part of tea saponin.
The dosage of the zymophyte agent is 3 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2 hundred million/g; and the fermentation bacteria agent is obtained by mixing saccharomyces cerevisiae bacteria liquid, acetobacter pasteurianus AS1.41 bacteria liquid, bacillus subtilis ATCC 6633 bacteria liquid, actinomycetes 710 bacteria liquid, lactobacillus DJ8A bacteria liquid and trichoderma reesei JC40360 bacteria liquid according to the volume ratio of 3:3:1:0.7:0.7: 0.5.
The dosage of the effective microbial inoculum is 2wt per thousand of the total weight of the base fertilizer, the effective viable count (cfu) of the effective microbial inoculum is 0.20 hundred million/g, and the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633 liquid, bacillus licheniformis W10 liquid, bacillus laterosporus strain YSC3 liquid, bacillus megaterium L2 liquid, bacillus amyloliquefaciens LX1 liquid, trichoderma harzianum T-22 liquid and paecilomyces lilacinus IPC liquid according to the volume ratio of the bacterial liquids of 1:1:1:1:1:1: 1.
Example 6
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
45 parts of municipal biogas residues, 40 parts of cow dung (with the water content of 50 wt%), 2.5 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
The conditioner is specifically 2.5 parts of saw dust which is completely crushed.
The heavy metal passivator is 0.5 part of fly ash.
The antagonistic pathogenic bacteria is specifically 0.03 part of tea saponin.
The dosage of the zymophyte agent is 1.5 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2 hundred million/g; and the zymophyte agent is saccharomyces cerevisiae bacterial liquid.
The dosage of the effective microbial inoculum is 1.5wt per mill of the total weight of the base fertilizer, the effective viable count (cfu) of the effective microbial inoculum is 0.2 hundred million/g, and the effective microbial inoculum is bacillus subtilis ATCC 6633 bacterial solution.
Example 7
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
45 parts of municipal biogas residues, 45 parts of chicken manure, 4 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
Wherein the livestock and poultry manure is specifically 35 parts of cow manure (with the water content of 50 wt%) and 20 parts of chicken manure (with the water content of 70 wt%).
The conditioner is specifically 1 part of thoroughly crushed sawdust, 1.5 parts of peanut shell powder and 1.5 parts of straws.
The heavy metal passivator is 0.4 part of fly ash.
The antagonistic pathogenic bacteria is specifically 0.03 part of tea saponin.
The dosage of the zymophyte agent is 5 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2 hundred million/g; and the fermentation microbial inoculum is obtained by mixing saccharomyces cerevisiae bacterial liquid, acetobacter pasteurianus AS1.41 bacterial liquid and bacillus subtilis ATCC 6633 bacterial liquid according to the volume ratio of 3:3:3: 1.
The using amount of the effective microbial inoculum is 2.5 weight per mill of the total weight of the base fertilizer, the effective viable count (cfu) of the effective microbial inoculum is 0.20 hundred million/g, and the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633 liquid, bacillus licheniformis W10 liquid and bacillus laterosporus strain YSC3 liquid according to the bacterial liquid volume ratio of 1:1: 1.
Example 8
The embodiment provides a biological organic fertilizer based on municipal biogas residues and livestock and poultry manure, which comprises a basic fertilizer, a fermentation microbial inoculum and an efficacy microbial inoculum;
the base fertilizer comprises the following components in parts by weight:
45 parts of municipal biogas residues, 55 parts of livestock and poultry manure, 5 parts of a conditioner, 0.6 part of a heavy metal passivator and 0.04 part of antagonistic pathogenic bacteria.
Wherein the livestock and poultry manure is specifically 35 parts of cow manure (with the water content of 50 wt%) and 20 parts of chicken manure (with the water content of 70 wt%);
the conditioner is prepared from thoroughly pulverized straw 1 part, mushroom residue 1 part, Chinese medicinal residue 1 part, tobacco powder 1 part, and pericarp and vegetable leaf 1 part.
The heavy metal passivator is 0.6 part of fly ash.
The antagonistic pathogenic bacteria is specifically 0.04 part of tea saponin.
The dosage of the zymophyte agent is 4 per mill of the total weight of the basic fertilizer, and the effective viable count (cfu) of the zymophyte agent is 2 hundred million/g; and the fermentation inoculum is obtained by mixing bacillus subtilis ATCC 6633 bacterial liquid, actinomycete 710 bacterial liquid, lactobacillus DJ8A bacterial liquid and trichoderma reesei JC40360 bacterial liquid according to the volume ratio of 3:1:0.7:0.7: 0.5.
The dosage of the effective microbial inoculum is 2wt per thousand of the total weight of the base fertilizer, the effective viable count (cfu) of the effective microbial inoculum is 0.20 hundred million/g, and the effective microbial inoculum is prepared by mixing bacillus megatherium L2 liquid, bacillus amyloliquefaciens LX1 liquid, trichoderma harzianum T-22 liquid and paecilomyces lilacinus IPC liquid according to the bacterial liquid volume ratio of 1:1:1: 1.
The bacterial liquid is obtained according to the following methods:
and (3) inoculating a ring of the saccharomyces cerevisiae strain preserved on the solid inclined plane to a liquid YPDA culture medium (20g of peptone, 10g of yeast extract, 2g of glucose, a proper amount of double-distilled water for dissolution, 15ml of 0.2% adenine solution, constant volume of 1.0L, autoclaving at 113 ℃ for 30min, storing at room temperature), culturing at 30 ℃, and at 180rpm for strain rejuvenation, and then carrying out passage with 1% inoculation amount until the liquid YPDA culture medium is cultured for about 48 hours under the same condition.
Inoculating Acetobacter pasteurianus AS1.41 strain to SICC0009 Acetobacter aceti liquid culture medium-II (200 ml of bean sprout juice, 10g of glucose, CaCO)320g of ethanol, 20ml of ethanol, 1L of distilled water and ethanol added after sterilization) are cultured at the constant temperature of 30 ℃ and 200rpm for strain rejuvenation, then subcultured to the liquid culture medium by 1 percent of inoculum size and cultured for about 8-9 days under the same conditions.
Bacillus subtilis ATCC 6633 bacterial liquid Bacillus subtilis was inoculated into 250ml of sterile broth medium. Culturing at 37 deg.C for 48h on reciprocating shaking table, heating at 65 deg.C, and vibration culturing for 15min to make spore concentration reach average 1.5 × 109mL, incubate the culture at 37 ℃ for 48h, and if spores are to be used immediately, dilute the spore suspension with PBS to obtain a concentration of 5-8 × 108Perml spore suspension.
Inoculating the spore of the actinomycete 710 strain on the slant of the Gao-shi culture medium I by using an inoculating loop in a Z-shaped manner, and culturing at 28 ℃ for 7 days to obtain a strain to be fermented; inoculating 3-5 rings of sterile inoculating loop into 100mL liquid fermentation medium (glucose 10g, peptone 30g, NaCl 2.5g, CaCO)3 2.0g、H2O1.0L, millet seed soaking 10.4g) in a 250mL Erlenmeyer flask, shaking at 25 ℃ and 250rpmAnd (4) culturing for 4 d.
Inoculating Lactobacillus DJ8A strain to MRS culture medium (casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween 801.0g, K2HPO42.0g，MgSO4·7H2O 0.2g，MnSO4·H2O 0.05g，CaCO320.0g, 1.0L of distilled water, pH6.8), 37 ℃, and 200rpm reciprocal shaking culture for 24h rejuvenation, subculturing to the above liquid medium at an inoculum size of 1%, and culturing under the same conditions for about 24 h.
Trichoderma reesei JC40360 bacterial liquid, namely inoculating Trichoderma reesei (Trichoderma reesei) into a PDA culture medium, and performing activation culture at 26 ℃ for 12-24 h to obtain an activated strain; inoculating the obtained activated strain into seed culture medium (peeled potato 200g, glucose 20g, KH)2PO4 3g，MgSO4·7H2O1.5 g, mixing the above components, adding 1.0L of water, boiling for 30min, filtering to remove potato pieces, adding the filtrate to 1.0L), and performing proliferation culture at 26 deg.C for 24-36 h to obtain seed solution.
Example 9
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of a base fertilizer according to the proportion of example 1, adding a zymophyte agent according to 3wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stacking size is 0.55m wide at the upper part, 2m wide at the lower part and 1.3m high at the lower part;
step two, after the first mixed material is stacked and arranged, covering a composite polymer film (the moisture permeability of which is 8000 g/m) on the top of the first mixed material224h, air permeability of 2L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 15 days in an environment with the fermentation temperature of 65 ℃ and the oxygen concentration of 13% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 10 days to reduce the water content of the base fertilizer to 20 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 2 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Example 10
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of a base fertilizer according to the proportion of example 2, adding a zymophyte agent according to 3wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stacking size is 0.55m wide at the upper part, 2m wide at the lower part and 1.2m high at the lower part;
step two, after the first mixed material is stacked and arranged, covering a composite polymer film (the moisture permeability of which is 8500 g/m) on the top of the first mixed material224h, air permeability of 2.5L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 20 days in an environment with the fermentation temperature of 60 ℃ and the oxygen concentration of 8% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 14 days to reduce the water content of the base fertilizer to 25 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 2 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Example 11
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of the base fertilizer according to the proportion of the embodiment 3, adding a zymophyte agent according to 3wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stacking size is 0.55m wide at the upper part, 2m wide at the lower part and 1.5m high at the lower part;
step two, after the first mixed material stack is completely placed, covering the top of the first mixed material stackCover composite polymer film (moisture permeability of 8300 g/m)224h, air permeability of 2.3L/m2(s), compacting all around to prevent gas from escaping, and continuously fermenting for 10 days in an environment with the fermentation temperature of 62 ℃ and the oxygen concentration of 18% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 7 days to reduce the water content of the base fertilizer to 30 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 2 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Example 12
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of the base fertilizer according to the proportion of the embodiment 4, adding a zymophyte agent according to 3wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stacking size is 0.55m wide at the upper part, 2m wide at the lower part and 1.3m high at the lower part;
step two, after the first mixed material is stacked and arranged, covering a composite polymer film (the moisture permeability of which is 8000 g/m) on the top of the first mixed material224h, air permeability of 2L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 15 days in an environment with the fermentation temperature of 65 ℃ and the oxygen concentration of 13% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 10 days to reduce the water content of the base fertilizer to 20 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 2 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Example 13
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of the base fertilizer according to the proportion of the embodiment 5, adding a zymophyte agent according to 3wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stacking size is 0.55m wide at the upper part, 2m wide at the lower part and 1.3m high at the lower part;
step two, after the first mixed material is stacked and arranged, covering a composite polymer film (the moisture permeability of which is 8000 g/m) on the top of the first mixed material224h, air permeability of 2L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 15 days in an environment with the fermentation temperature of 65 ℃ and the oxygen concentration of 13% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 10 days to reduce the water content of the base fertilizer to 20 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 2 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Example 14
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of the base fertilizer according to the proportion of the embodiment 6, adding a zymophyte agent according to 1.5wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stack size is 0.55m wide at the upper part, 2m wide at the lower part and 1.3m high at the lower part;
step two, after the first mixed material is stacked and arranged, covering a composite polymer film (the moisture permeability of which is 8000 g/m) on the top of the first mixed material224h, air permeability of 2L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 15 days in an environment with the fermentation temperature of 65 ℃ and the oxygen concentration of 13% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 10 days to reduce the water content of the base fertilizer to 20 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 1.5 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Example 15
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of the base fertilizer according to the proportion of the embodiment 7, adding a zymophyte agent according to 5wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stacking size is 0.55m wide at the upper part, 2m wide at the lower part and 1.3m high at the lower part;
step two, after the first mixed material is stacked and arranged, covering a composite polymer film (the moisture permeability of which is 8000 g/m) on the top of the first mixed material224h, air permeability of 2L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 15 days in an environment with the fermentation temperature of 65 ℃ and the oxygen concentration of 13% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 10 days to reduce the water content of the base fertilizer to 20 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 2.5 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Example 16
The embodiment provides a preparation method of a bio-organic fertilizer, which comprises the following steps:
step one, weighing each component of the base fertilizer according to the proportion of the embodiment 8, adding a zymophyte agent according to 4wt per mill of the total weight of the base fertilizer, uniformly mixing to obtain a first mixed material, and stacking the obtained first mixed material in a short stack mode, wherein the stacking size is 0.55m wide at the upper part, 2m wide at the lower part and 1.3m high at the lower part;
step two, firstAfter the mixed materials are stacked and arranged, the top of the mixed materials is covered with a composite polymer film (the moisture permeability of the composite polymer film is 8000 g/m)224h, air permeability of 2L/m2And/s), compacting all around to prevent gas from escaping, and continuously fermenting for 15 days in an environment with the fermentation temperature of 65 ℃ and the oxygen concentration of 13% to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 10 days to reduce the water content of the base fertilizer to 20 wt% of the weight of the base fertilizer, thereby obtaining a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum according to 2 wt% of the total weight of the base fertilizer, and uniformly mixing to obtain the biological organic fertilizer.
Experimental part
Firstly, heavy metal detection is carried out on each raw material component
According to the corresponding regulation in the national standard (NY884-2012) of the bio-organic fertilizer, biogas residues, cow dung and chicken manure samples of a municipal sewage treatment plant for fermenting and composting are sent to a third-party authoritative detection mechanism for content determination of five heavy metals including As, Cd, Pb, Cr and Hg, so that a party without heavy metal pollution can be used for preparing the organic fertilizer.
In order to explore the quality condition of the organic fertilizer, the organic fertilizer prepared in example 9 is used as an example, and the physical, chemical and biological indexes of the organic fertilizer are detected as follows:
the method comprises the steps of dividing a material stack into an upper layer and a lower layer by adopting a five-point sampling method, selecting a central point of the upper layer and a middle part of the lower layer at a collecting point, collecting samples of 4 peripheral parts after removing materials with the thickness of 20cm on the surface, wherein each sample is about 500g, and measuring the physical, chemical and biological indexes of a product according to corresponding regulations in the national standard (NY884-2012) of the bio-organic fertilizer.
The results are shown in Table 1.
TABLE 1
According to the table 1, the requirements of the physicochemical and biological indexes of the product are determined according to the corresponding regulations in national standard (NY884-2012) of the bio-organic fertilizer composite.
The production of the biological organic fertilizer selects biogas residues generated after high-dry anaerobic fermentation of a municipal sewage treatment plant for producing biogas as a main raw material, simultaneously takes the surrounding livestock and poultry breeding manure as a secondary utilizable resource for aerobic composting, adds a certain amount of organic fertilizer fermentation microbial inoculum, converts biodegradable organic matters in the raw material into stable humus by utilizing microbial catabolism, and effectively kills parasites, pathogenic bacteria and weed seeds in the biogas residues and the livestock and poultry manure at continuous high temperature in the fermentation process, thereby achieving the purposes of harmless and resource utilization of the biogas residues and the livestock and poultry manure. The organic fertilizer product is compounded with a certain amount of compound high-efficiency microbial effect microbial inoculum, the prepared biological organic fertilizer product not only contains higher effective viable bacteria quantity, but also has the advantages that the functional strains can effectively improve the soil microenvironment and improve the capability of crops for absorbing nutrient components, and antibiotics, antibacterial proteins and active polypeptides secreted by various biocontrol bacteria can promote the growth of crops on one hand and effectively resist soil-borne diseases on the other hand.
(II) implementing the plant growth promoting effect:
1) material
The plant to be tested is tomato, and the name is Pingdu Jinguanwang, which is purchased from Henan Yu skill science development Co. The experimental plot is located in the Pingyu county of Temmah shop, Henan province.
2) Carrying out the step
A total of 3 parallel experiments were designed.
A first group: organic Fertilizer (OF) without adding effective microbial inoculum and conventional fertilization
The Organic Fertilizer (OF) without the addition OF the efficacy microbial inoculum is obtained by removing the efficacy microbial inoculum on the basis OF the organic fertilizer in example 1. In the experimental group, when the tomatoes are planted, the OF is mixed with soil according to 120kg per mu for base application, and in addition, the P is applied in holes per mu2O56 kg、K2SO47kg, and applying 3kg of urea after 5 days of field planting.
Second group: application of Bio-organic fertilizer (BOF) plus conventional fertilization
The organic fertilizer (BOF) in example 1 is adopted as the biological organic fertilizer, and when the tomatoes are planted in the experimental group, the BOF is mixed with soil according to 120kg per mu for base application, and in addition, P is hole-applied per mu2O56 kg、K2SO47kg, and applying 3kg of urea after 5 days of field planting.
Third group: using conventional fertilizer application as blank control group
The experimental group is applied in P holes per mu when the tomatoes are planted2O56 kg、K2SO47kg, and 3kg of urea is added after 5 days of permanent planting.
The three experimental groups are planted in a ridging mode, the height of each ridge is about 0.3m, the distance between plants is 0.3m, the row spacing is 0.3m, the plants are planted in double rows, and 16 plants are planted in each row.
Healthy tomato seedlings with good growth condition and consistent size are planted after the tomato seeds are fertilized by the three experimental groups respectively. And after 3-5d, observing timely replanting according to the root fixing and survival conditions of the seedlings. And measuring the height and stem thickness of the seedlings after 15 days of planting, and measuring the height, stem thickness, branch number of each plant and fruit yield of the plants after the plants enter a mature period.
3) Results and analysis
TABLE 2 growth promoting effect of bio-organic fertilizer (BOF) on tomato seedlings
Wherein, a, b and c represent the difference of each data at the significance level of P <0.05 after the data are analyzed by SPSS for difference significance.
Table 2 shows that compared with a control, the plant height of tomato seedlings can be increased by 22.27% by adding the bio-organic fertilizer (BOF) added with the composite efficacy microbial inoculum and conventional fertilization, and the stem thickness can be increased by 29.49%.
TABLE 3 influence of Bio-organic fertilizer (BOF) on the trait and yield of mature tomato plant
Wherein, a, b and c represent the difference of each data at the significance level of P <0.05 after the data are analyzed by SPSS for difference significance.
As can be seen from the results in Table 3, after the tomato plants enter the maturity period, the promotion effect of the bio-organic fertilizer (BOF) on the plant height is obviously weakened, the promotion effect on the increase of the stem thickness and the branch number of a single plant is obvious, and the fruit yield of the single plant is obviously increased by 23.61%.
Examples of the soil improvement effect:
collecting soil samples of 0-20 cm deep plough layers at different fertilization treatment point positions 1 week before tomato planting and 1 week before tomato harvesting, respectively, and measuring the contents of organic matters, pH, water-soluble salts, total nitrogen, available phosphorus and quick-acting potassium after air drying, grinding and sieving.
As is evident from Table 4, the organic matter OF the soil can be increased by applying both Organic Fertilizer (OF) and Biological Organic Fertilizer (BOF), and the organic matter OF the soil treated by BOF is increased to 12.84% at most; from the perspective OF pH change, both OF and BOF application contribute to the reduction OF soil alkalinity, both OF which lead to a reduction in Cation Exchange Capacity (CEC), and contribute to the improvement OF soil salinization; after the OF and BOF are applied, the total nitrogen content, the available phosphorus content and the available potassium content OF the soil are increased, particularly, the BOF treatment enables the nutrient increment OF each soil to be obvious, and the total nitrogen content, the available phosphorus content and the available potassium content are respectively increased by 8.90%, 9.47% and 12.61%.
TABLE 4 influence of the Bio-organic fertilizer (BOF) on the physicochemical indices of the soil for tomato cultivation
Wherein, a, b and c represent the difference of each data at the significance level of P <0.05 after the data are analyzed by SPSS for difference significance.
It is to be understood that the above-described embodiments are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (10)
1. A bio-organic fertilizer based on municipal biogas residues and livestock and poultry manure is characterized by comprising a basic fertilizer, a fermentation microbial inoculum and an effective microbial inoculum; wherein the base fertilizer comprises the following components in parts by weight:
45-55 parts of municipal biogas residues, 40-60 parts of livestock and poultry manure, 1-5 parts of a conditioner, 0.4-0.6 part of a heavy metal passivator and 0.03-0.04 part of antagonistic pathogenic bacteria.
2. The bio-organic fertilizer according to claim 1, wherein the amount of the fermentation inoculum is 1.5-5 wt% of the base fertilizer, and the effective viable count (cfu) of the fermentation inoculum is more than or equal to 2 hundred million/g;
the dosage of the effective microbial inoculum is 1.5-2.5 wt% of the base fertilizer, and the effective viable count (cfu) of the effective microbial inoculum is more than or equal to 0.20 hundred million/g.
3. The bio-organic fertilizer according to claim 2, wherein the fermentation inoculum is one or more of saccharomyces cerevisiae, acetobacter pasteurianus AS1.41, bacillus subtilis ATCC 6633, actinomycetes 710, lactobacillus DJ8A and trichoderma reesei JC 40360;
the effective microbial inoculum is one or more of bacillus subtilis ATCC 6633, bacillus licheniformis W10, bacillus laterosporus strain YSC3, bacillus megaterium L2, bacillus amyloliquefaciens LX1, trichoderma harzianum T-22 and paecilomyces lilacinus IPC.
4. The bio-organic fertilizer AS claimed in claim 3, wherein the fermentation bacteria agent is prepared by mixing saccharomyces cerevisiae, acetobacter pasteurianus AS1.41, bacillus subtilis ATCC 6633, actinomycetes 710, lactobacillus DJ8A and trichoderma reesei JC40360 according to the bacteria liquid volume ratio of 3:3:1:0.7:0.7: 0.5.
5. The bio-organic fertilizer as claimed in claim 3, wherein the effective microbial inoculum is prepared by mixing bacillus subtilis ATCC 6633, bacillus licheniformis W10, bacillus laterosporus strain YSC3, bacillus megaterium L2, bacillus amyloliquefaciens LX1, trichoderma harzianum T-22 and paecilomyces lilacinus IPC according to a bacterial liquid volume ratio of 1:1:1:1:1:1: 1.
6. The bio-organic fertilizer according to claim 1, wherein the conditioner is one or more of sawdust, peanut vine, peanut shell powder, straw, mushroom dregs, herb residues, tobacco powder and pericarp and vegetable leaves.
7. The bio-organic fertilizer according to claim 1, wherein the heavy metal passivator is one or more of fly ash, lime and turfy soil; the antagonistic pathogenic bacteria are tea saponin.
8. The bio-organic fertilizer according to claim 1, wherein the municipal biogas residue is biogas residue obtained by highly dry anaerobic fermentation of municipal sludge to produce biogas.
9. The bio-organic fertilizer as claimed in claim 1, wherein the livestock manure is one or more of cow manure and chicken manure; the water content of the cow dung is 45-55 wt%, and the water content of the chicken manure is 65-75 wt%.
10. A method for preparing a bio-organic fertilizer according to any one of claims 1 to 9, comprising the following steps:
step one, weighing each component of a base fertilizer, uniformly mixing, adding a zymophyte agent, and uniformly mixing again to obtain a first mixed material;
placing the first mixed material in an environment with the temperature not higher than 65 ℃ and the oxygen concentration of 8% -18% for continuous fermentation for 10-20 days to obtain a second mixed material;
after the second mixed material is obtained, stopping supplying oxygen, aging and decomposing, and continuously fermenting for 7-14 days to reduce the water content of the base fertilizer to 20-30 wt% of the base fertilizer, so as to obtain a third mixture;
and step four, crushing and sieving the third mixture, adding an effective microbial inoculum and uniformly mixing to obtain the biological organic fertilizer.
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| CN110591966A (en) * | 2019-09-30 | 2019-12-20 | 四川明湖环保科技有限公司 | Environment deodorization composite microbial inoculum and preparation method thereof |
| CN115340420A (en) * | 2022-06-20 | 2022-11-15 | 南京宁粮生物工程有限公司 | Soil conditioner for reducing heavy metal content of crops and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110591966A (en) * | 2019-09-30 | 2019-12-20 | 四川明湖环保科技有限公司 | Environment deodorization composite microbial inoculum and preparation method thereof |
| CN110591966B (en) * | 2019-09-30 | 2023-09-29 | 四川明湖环保科技有限公司 | Environment deodorization composite microbial inoculant and preparation method thereof |
| CN115340420A (en) * | 2022-06-20 | 2022-11-15 | 南京宁粮生物工程有限公司 | Soil conditioner for reducing heavy metal content of crops and preparation method thereof |
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