CN111876359A - Compound microbial inoculum for recycling vegetable residues and preparation method and application thereof - Google Patents
Compound microbial inoculum for recycling vegetable residues and preparation method and application thereof Download PDFInfo
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- C05F11/00—Other organic fertilisers
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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Abstract
The invention discloses a compound microbial inoculum for recycling vegetable residues and a preparation method and application thereof, wherein the compound microbial inoculum is prepared from bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid, bacillus cereus bacterial liquid and gordonia bacterial liquid; wherein the effective viable bacteria content of the bacillus bh1 bacterial liquid, the bacillus bh2 bacterial liquid, the bacillus bh3 bacterial liquid and the bacillus cereus bacterial liquid is more than or equal to 1010CFU/ml(ii) a The effective viable bacteria content of the Gordoniae bacterial liquid is more than or equal to 109CFU/ml. The composite microbial inoculum can accelerate the composting speed of the straws, and improve the decomposition degree and the regeneration and utilization rate of resources.
Description
Technical Field
The invention belongs to the technical field of microbial fermentation, and particularly relates to a compound microbial inoculum for recycling vegetable residues, and a preparation method and application thereof.
Background
At present, a large amount of vegetable residues are generated in places such as vegetable greenhouses, farmer markets and the like every day, and greenhouse growers and pedlars mostly stack the vegetable residues on the field and decay the vegetable residues or throw the vegetable residues into a garbage can and finally transport the vegetable residues to a landfill site for landfill. Improper disposal of vegetable residues causes severe pollution to rural and urban environments. The processing cost of outward transportation and landfill is increased due to the fact that the vegetable residues generated every day are various in types, large in quantity and heavy in weight.
In fact, the vegetable residues contain a certain amount of various elements such as carbon, nitrogen, phosphorus, potassium, calcium and the like, and also contain rich organic matters such as cellulose and hemicellulose, so that the vegetable residues have huge potential wealth, and great economic value can be created if scientific development and utilization are carried out. Therefore, the method has very important significance for more reasonably and resourcefully treating the vegetable residues.
In summary, a new complex microbial inoculum for recycling vegetable residues, a preparation method and application thereof are needed.
Disclosure of Invention
The invention aims to provide a compound microbial inoculum for recycling vegetable residues as well as a preparation method and application thereof, so as to solve one or more technical problems. The composite microbial inoculum can accelerate the composting speed of the straws, and improve the decomposition degree and the regeneration and utilization rate of resources.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention relates to a compound microbial inoculum for recycling vegetable residues, which is prepared from bacillus bh1, bacillus bh2, bacillus bh3, bacillus cereus and gordonia.
The invention has the further improvement that the bacillus strain is prepared from bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid, bacillus cereus bacterial liquid and gordonia bacterial liquid; wherein the budsThe effective viable bacteria content of bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid and bacillus cereus bacterial liquid is more than or equal to 1010CFU/ml; the effective viable bacteria content of the Gordoniae bacterial liquid is more than or equal to 109CFU/ml。
The compound microbial inoculum is used for composting vegetable residues.
The application of the compound microbial inoculum comprises the following steps:
(1) crushing the vegetable residue to be less than 2 cm to obtain crushed vegetable residue;
(2) mixing the crushed vegetable residues obtained in the step (1) with the compound microbial inoculum according to the volume ratio of 150: 1, uniformly stirring, fermenting for 1-2 weeks, and completing the resource treatment of the vegetable residues.
The invention is further improved in that in the step (1), the vegetable residues are crushed to 1-2 cm.
A further improvement of the invention is that the vegetable residue in step (1) comprises: cabbage residue, baby cabbage residue.
The preparation method of the compound microbial inoculum comprises the following steps:
1) respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3, bacillus cereus and gordonia to a culture medium, and culturing to obtain bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid, bacillus cereus bacterial liquid and gordonia bacterial liquid;
2) mixing the five bacterial liquids obtained in the step 1) according to an equal volume ratio to obtain the compound microbial inoculum.
The further improvement of the invention is that in the step 1), the effective viable bacteria content of the bacillus bh1 bacterial liquid, the bacillus bh2 bacterial liquid, the bacillus bh3 bacterial liquid and the bacillus cereus bacterial liquid is more than or equal to 1010CFU/ml; the effective viable bacteria content of the Gordoniae bacterial liquid is more than or equal to 109CFU/ml。
In a further development of the invention, in step 1),
respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus on an LB liquid culture medium, culturing at the temperature of 35-37 ℃ and the rotating speed of 130-150 rpm for 40-50 hours to obtain bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid and bacillus cereus bacterial liquid;
inoculating the Gordonia on a beef extract-peptone liquid culture medium, and culturing at the temperature of 30 ℃ and the rotation speed of 100-120 rpm for 60-70 h to obtain Gordonia bacterial liquid.
In a further development of the invention, in step 1),
the LB liquid culture medium is that 10g of tryptone, 5g of yeast extract powder and 10g of sodium chloride are put into each 1000mL of deionized water;
the beef extract-peptone liquid culture medium is characterized in that 10g of beef extract, 10g of peptone and 5g of sodium chloride are added into every 1000mL of deionized water.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the microbial species is selected to prepare the compound microbial inoculum, and the microbial inoculum can accelerate the composting speed of vegetable residues, improve the decomposition degree and the resource utilization rate; the composting process is basically free of odor pollution; indexes such as total phosphorus, total nitrogen, nitrate nitrogen, total potassium and the like of the produced liquid fertilizer are obviously improved by 2-3 times compared with those of the liquid fertilizer without the microbial inoculum; wherein the nitrate nitrogen is about 3 times, which shows that the added microbial inoculum has good degradation capability and good nitrogen fixation effect. Meanwhile, the invention can solve the problem of difficult transportation and storage of the vegetable residues and has good environmental effect.
The preparation method of the compound microbial inoculum is simple, low in production cost and free of pollution to the environment.
Detailed Description
The compound microbial inoculum for the resource treatment of the vegetable residues comprises the following components: bacillus bh1, bacillus bh2, bacillus bh3, bacillus cereus and gordonia.
The complex microbial inoculum of the embodiment of the invention comprises: the effective viable bacteria content of the bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus liquid is not less than 1010CFU/ml, Gordoniella strain solutionThe content of effective viable bacteria is not less than 109CFU/ml。
The preparation method of the compound microbial inoculum for the resource treatment of the vegetable residues comprises the following steps:
(1) respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus on 150ml LB liquid culture medium, culturing at the temperature of 35-37 ℃ and the rotating speed of 130-150 rpm for 40-50 hours to obtain bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus suspension;
(2) inoculating Gordonia on 150ml beef extract-peptone liquid culture medium, culturing at 30 ℃ and 100-120 rpm for 60-70 h to obtain Gordonia liquid;
(3) and mixing the five microbial inoculums according to the same volume to obtain the compound microbial inoculums. Wherein the strain can be purchased from Yulin desert King Biotech Co., Ltd.
In the embodiment of the invention, the LB culture medium comprises: 10g of tryptone, 5g of yeast extract powder, 0g of sodium chloride and 1000mL of deionized water.
In the embodiment of the invention, the beef extract-peptone culture medium comprises: 10g of beef extract, 10g of peptone, 5g of sodium chloride and 1000mL of deionized water.
The application of the compound microbial inoculum for the resource treatment of the vegetable residues comprises the following steps:
(1) crushing the vegetable residues to 1-2 cm;
(2) placing the crushed vegetable residues in a composting barrel, and mixing the vegetable residues with a compound microbial inoculum according to the volume ratio of 150: 1 into a compost bucket;
(3) and (3) uniformly stirring the vegetable residues in the composting barrel, and fermenting for 1-2 weeks.
To sum up, the complex microbial inoculum of the embodiment of the invention comprises: bacillus bh1, bacillus bh2, bacillus bh3, bacillus cereus and gordonia; in the complex microbial inoculum: the effective viable bacteria content of the bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus liquid is not less than 1010CFU/ml, Gordoniella strain solutionThe content of effective viable bacteria is not less than 109CFU/ml. The invention also discloses a preparation method of the compound microbial inoculum, which comprises the steps of mixing the microbial inoculum prepared by the five bacteria in the same volume to obtain the compound microbial inoculum; the complex microbial inoculum can accelerate the composting speed, the decomposition degree and the resource regeneration and utilization rate of vegetable residues; meanwhile, the problem of environmental pollution caused by improper treatment of vegetable residues is solved. According to the invention, the novel microorganism species is selected to prepare the compound microbial inoculum, the microbial inoculum can be used for accelerating the composting speed, the decomposition degree and the resource utilization rate of the vegetable residues, and the composting process is basically free of odor pollution; meanwhile, the difficulty in transporting and storing the vegetable residues is solved, and the environment benefit is good; the preparation method of the invention is simple, has low production cost and no pollution to the environment.
Example 1
The preparation method of the compound microbial inoculum for the resource treatment of the vegetable residues comprises the following steps:
the LB medium comprises: 10g of tryptone, 5g of yeast extract powder, 0g of sodium chloride and 1000mL of deionized water.
The beef extract-peptone culture medium comprises: 10g of beef extract, 10g of peptone, 5g of sodium chloride and 1000mL of deionized water.
(1) Respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus on 150ml LB liquid culture medium, culturing at 35-37 deg.C and 130rpm for 48 hr to obtain effective viable bacteria content of 5 × 1010CFU/ml bacillus bh1, effective viable bacteria content 3X 1011CFU/ml bacillus bh2, effective viable bacteria content 4X 1010CFU/ml bacillus bh3, effective viable bacteria content 5X 1011CFU/ml Bacillus cereus suspension;
(2) inoculating Gordonia onto 150ml beef extract-peptone liquid culture medium, culturing at 30 deg.C and 100rpm for 70 hr to obtain effective viable bacteria content of 5 × 109CFU/ml Gordoniae bacterial liquid; and mixing the five microbial inoculum according to the same volume to obtain the compound microbial inoculum.
The application of the compound microbial inoculum in the embodiment of the invention in the cabbage resource treatment comprises the following steps:
(1) crushing cabbage to 1-2 cm;
(2) putting the crushed cabbage into a composting barrel, and mixing the cabbage with the compound microbial inoculum according to the volume ratio of 150: 1 into a compost bucket;
(3) the cabbage in the compost bucket is evenly stirred and fermented for 15 days.
Example 2
The preparation method of the compound microbial inoculum for recycling the baby cabbage comprises the following steps:
the LB medium comprises: 10g of tryptone, 5g of yeast extract powder, 0g of sodium chloride and 1000mL of deionized water.
The beef extract-peptone culture medium comprises: 10g of beef extract, 10g of peptone, 5g of sodium chloride and 1000mL of deionized water.
(1) Respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus on 150ml LB liquid culture medium, culturing at 35-37 deg.C and 140rpm for 50 hr to obtain effective viable bacteria content of 6 × 1011CFU/ml bacillus bh1, effective viable bacteria content 4X 1010CFU/ml bacillus bh2, effective viable bacteria content 5X 1010CFU/ml bacillus bh3, effective viable bacteria content 2X 1011CFU/ml Bacillus cereus suspension;
(2) inoculating Gordonia onto 150ml beef extract-peptone liquid culture medium, culturing at 30 deg.C and 110rpm for 68 hr to obtain effective viable bacteria content of 4 × 1010CFU/ml Gordoniae bacterial liquid; and mixing the five microbial inoculums according to the same volume to obtain the compound microbial inoculums.
The application of the compound microbial inoculum in the recycle treatment of the baby cabbage comprises the following steps:
(1) crushing baby cabbage to 1-2 cm;
(2) putting the crushed baby cabbage into a composting barrel, and mixing the baby cabbage with the compound microbial inoculum according to the volume ratio of 150: 1 into a compost bucket;
(3) stirring the baby cabbage in the composting barrel uniformly, and fermenting for 21 days.
According to the relevant indexes of organic fertilizers in agricultural standards NY525-2012 and DB33-T of the organic fertilizers, the biological organic fertilizer is produced by fermenting vegetable residues by using the composite fermentation inoculant provided by the invention, and the sampling and measuring result after 14 days of compost aerobic fermentation is as follows: the effective viable count of the microorganism is 10 by a plate counting method after the microorganism fermentation11The CFU/g is far higher than the standard of more than or equal to 0.2 hundred million/g in the organic fertilizer; the pH value is 5.6 and is between 5.5 and 8.5, the determination of faecal coliform group refers to GB/T19524.1, the faecal coliform group is 0 per gram (mL) and is less than or equal to 100 per gram (mL), and all indexes all meet the nutritional requirements of the organic fertilizer.
Example 3
The compound microbial inoculum for recycling vegetable residues is prepared from a bacillus bh1 bacterial solution, a bacillus bh2 bacterial solution, a bacillus bh3 bacterial solution, a bacillus cereus bacterial solution and a gordonia bacterial solution; wherein the effective viable bacteria content of the bacillus bh1 bacterial liquid, the bacillus bh2 bacterial liquid, the bacillus bh3 bacterial liquid and the bacillus cereus bacterial liquid is 1010CFU/ml; the effective viable bacteria content of the Gordonia bacterium liquid is 109CFU/ml。
Example 4
The compound microbial inoculum for recycling vegetable residues is prepared from a bacillus bh1 bacterial solution, a bacillus bh2 bacterial solution, a bacillus bh3 bacterial solution, a bacillus cereus bacterial solution and a gordonia bacterial solution; wherein the effective viable bacteria content of the bacillus bh1 bacterial liquid, the bacillus bh2 bacterial liquid, the bacillus bh3 bacterial liquid and the bacillus cereus bacterial liquid is 5 multiplied by 1010CFU/ml; the effective viable bacteria content of Gordonia bacteria liquid is 5 multiplied by 109CFU/ml。
Example 5
The preparation method of the compound microbial inoculum of the embodiment of the invention comprises the following steps:
1) respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3, bacillus cereus and gordonia to a culture medium, and culturing to obtain bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid, bacillus cereus bacterial liquid and gordonia bacterial liquid;
2) mixing the five bacterial liquids obtained in the step 1) according to an equal volume ratio to obtain the compound microbial inoculum.
In the step 1), the effective viable bacteria content of the bacillus bh1 bacterial liquid, the bacillus bh2 bacterial liquid, the bacillus bh3 bacterial liquid and the bacillus cereus bacterial liquid is more than or equal to 1010CFU/ml; the effective viable bacteria content of the Gordoniae bacterial liquid is more than or equal to 109CFU/ml。
In the step 1), respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus on an LB liquid culture medium, and culturing for 40-50 hours at the temperature of 35-37 ℃ and the rotating speed of 130-150 rpm to obtain bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid and bacillus cereus bacterial liquid;
inoculating the Gordonia on a beef extract-peptone liquid culture medium, and culturing at the temperature of 30 ℃ and the rotation speed of 100-120 rpm for 60-70 h to obtain Gordonia bacterial liquid.
In the step 1), the LB liquid culture medium is prepared by adding 10g of tryptone, 5g of yeast extract powder and 10g of sodium chloride into every 1000mL of deionized water; the beef extract-peptone liquid culture medium is characterized in that 10g of beef extract, 10g of peptone and 5g of sodium chloride are added into every 1000mL of deionized water.
Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can make modifications and equivalents to the embodiments of the present invention without departing from the spirit and scope of the present invention, which is set forth in the claims of the present application.
Claims (10)
1. A compound microbial inoculum for recycling vegetable residues is characterized by being prepared from bacillus bh1, bacillus bh2, bacillus bh3, bacillus cereus and gordonia.
2. The compound microbial inoculum for resource treatment of vegetable residues according to claim 1, which is prepared from bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid, bacillus cereus bacterial liquid and gordonia bacterial liquid;
wherein the effective viable bacteria content of the bacillus bh1 bacterial liquid, the bacillus bh2 bacterial liquid, the bacillus bh3 bacterial liquid and the bacillus cereus bacterial liquid is more than or equal to 1010CFU/ml; the effective viable bacteria content of the Gordoniae bacterial liquid is more than or equal to 109CFU/ml。
3. The compound microbial inoculum of claim 1 or 2 is used for composting vegetable residues.
4. The application of the compound microbial inoculum of claim 1 or 2, which is characterized by comprising the following steps:
(1) crushing the vegetable residue to be less than 2 cm to obtain crushed vegetable residue;
(2) mixing the crushed vegetable residues obtained in the step (1) with the compound microbial inoculum according to the volume ratio of 150: 1, uniformly stirring, fermenting for 1-2 weeks, and completing the resource treatment of the vegetable residues.
5. The use according to claim 4, wherein in step (1), the vegetable residue is pulverized to 1-2 cm.
6. Use according to claim 4, wherein the vegetable residues in step (1) comprise: cabbage residue, baby cabbage residue.
7. The preparation method of the compound microbial inoculum of claim 1 is characterized by comprising the following steps:
1) respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3, bacillus cereus and gordonia to a culture medium, and culturing to obtain bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid, bacillus cereus bacterial liquid and gordonia bacterial liquid;
2) mixing the five bacterial liquids obtained in the step 1) according to an equal volume ratio to obtain the compound microbial inoculum.
8. The method according to claim 7, wherein in step 1), the effective viable bacteria content of the Bacillus bh1 bacteria liquid, the Bacillus bh2 bacteria liquid, the Bacillus bh3 bacteria liquid, and the Bacillus cereus bacteria liquid is 10 or more10CFU/ml; the effective viable bacteria content of the Gordoniae bacterial liquid is more than or equal to 109CFU/ml。
9. The method according to claim 7, wherein, in the step 1),
respectively inoculating bacillus bh1, bacillus bh2, bacillus bh3 and bacillus cereus on an LB liquid culture medium, culturing at the temperature of 35-37 ℃ and the rotating speed of 130-150 rpm for 40-50 hours to obtain bacillus bh1 bacterial liquid, bacillus bh2 bacterial liquid, bacillus bh3 bacterial liquid and bacillus cereus bacterial liquid;
inoculating the Gordonia on a beef extract-peptone liquid culture medium, and culturing at the temperature of 30 ℃ and the rotation speed of 100-120 rpm for 60-70 h to obtain Gordonia bacterial liquid.
10. The method according to claim 9, wherein, in step 1),
the LB liquid culture medium is that 10g of tryptone, 5g of yeast extract powder and 10g of sodium chloride are put into each 1000mL of deionized water;
the beef extract-peptone liquid culture medium is characterized in that 10g of beef extract, 10g of peptone and 5g of sodium chloride are added into every 1000mL of deionized water.
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