CN107653207A - One plant of secondary coccus apt to change and the method for high ammonia-nitrogen wastewater processing by-product single cell protein - Google Patents

One plant of secondary coccus apt to change and the method for high ammonia-nitrogen wastewater processing by-product single cell protein Download PDF

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CN107653207A
CN107653207A CN201711067819.XA CN201711067819A CN107653207A CN 107653207 A CN107653207 A CN 107653207A CN 201711067819 A CN201711067819 A CN 201711067819A CN 107653207 A CN107653207 A CN 107653207A
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high ammonia
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CN107653207B (en
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张小松
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Guangzhou Qingyuan Bio Technology Co. Ltd.
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Shenzhen Kegerui Environmental Biotechnology Co ltd
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Abstract

The present invention relates to biology and environmental technology field, it is therefore an objective to which providing one plant of secondary coccus apt to change and its method for high ammonia-nitrogen wastewater processing by-product single cell protein, the technical scheme of use is：One plant of hydrogen-oxidizing bacterium secondary coccus (Paracoccus versutus) D6 apt to change is provided, Chinese microorganism strain preservation conservator's common micro-organisms center (address was preserved on 05 12nd, 2017：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode：100101), deposit number is：CGMCC No.14119.The present invention be directed to the wasting of resources, secondary pollution problems existing for existing high ammonia-nitrogen wastewater treatment technology, there is provided secondary coccus D6 apt to change, COD and ammonia nitrogen and fixation CO can be removed in high ammonia-nitrogen wastewater2Produce single cell protein (SCP) while remove waste water stench.

Description

One plant of secondary coccus apt to change and for high ammonia-nitrogen wastewater processing by-product single cell protein Method
Technical field
The invention belongs to biology and environmental technology field, and in particular to one plant of secondary coccus apt to change newly screened, and the bacterium Single cell protein, the method for being directed to deodorizing waste water are produced using high ammonia-nitrogen wastewater.
Background technology
The industries such as chemical fertilizer, coking, petrochemical industry, pharmacy, food, refuse landfill can produce a large amount of high ammonia-nitrogen wastewaters, a large amount of ammonia Nitrogen waste water, which is discharged into water body, not only to be caused body eutrophication, causes black and odorous water, the difficulty and added cost of Water purification, even Toxic action is produced to crowd and biology, the handling process of ammonia nitrogen waste water mainly there are the various processing such as bioanalysis, physico-chemical process at present Technique, but these techniques are that ammonia nitrogen is shifted into water-outlet body into air or is converted into nitrogen discharge into air, without very Good realizes recycling.
In addition in recent years, China's livestock and poultry cultivation amount constantly expands, and because cultivation scale is big and scattered raiser is more, often produces per year Raw livestock and poultry feces incredible amount.Biogas engineering mitigates environmental pollution, hair as intensive livestock and poultry cultivation night soil recycling The important channel of ecologic breeding industry is opened up, was quickly grown in recent years.However, adjoint biogas engineering extensive development is biogas slurry Slag etc. significantly increases, and organic fertilizer is made because its is solid-state like in biogas residue, transports relatively convenient.But biogas slurry not only qualified discharge difficulty Greatly, processing cost is high, and easily causes secondary environmental pollution.Meanwhile CO2Not only can largely it be produced in anaerobic digestion process, And the CO of equivalent can be also generated after methyl hydride combustion utilization2, exacerbate global warming.
The content of the invention
The purpose of the present invention is asked for the wasting of resources, secondary pollution etc. existing for existing high ammonia-nitrogen wastewater treatment technology Topic, there is provided one plant can remove COD and ammonia nitrogen and fixed CO in high ammonia-nitrogen wastewater2Produce single cell protein (SCP) while remove useless The hydrogen-oxidizing bacterium of water stench.
To achieve the above object, present invention firstly provides one plant of hydrogen-oxidizing bacterium -- secondary coccus (Paracoccus apt to change Versutus) D6, Chinese microorganism strain preservation conservator common micro-organisms center (was preserved on 05 12nd, 2017 Location：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode：100101), deposit number For：CGMCC No.14119.
Secondary coccus (Paracoccusversutus) D6CGMCC No.14119 apt to change provided by the invention are imperial from Chengdu Screen and obtain in the soybean rhizosphere of spring post.Its physiological characteristic is：Cell is rod-short, long 0.5~1.1 μm, gram-negative Property, atrichia；Bacterium colony is rounded, and rat, the smooth of the edge is neat, and color is opaque milky；Grow pH be 5.0~ 9.0；Growth temperature is 25~35 DEG C；The bacterium is amphitrophy type, respectively with the organic carbon and ammonia in high ammonia-nitrogen wastewater during heterotrophism Nitrogen is that carbon source, nitrogen source are grown, and is carried out respectively by carbon source, nitrogen source of the ammonia nitrogen in carbon dioxide and high ammonia-nitrogen wastewater during autotrophy Growth, the bacterium can utilize the ammonia nitrogen production single cell protein in waste water under heterotrophism, autotrophic condition.
The invention provides the 16S rRNA qualification results of above-mentioned bacterial strains.Compared by BLAST, bacterial strain D6 and secondary ball apt to change The homology of bacterium (Paracoccus versutus) is 99%, therefore identifies that the bacterial strain is secondary coccus apt to change.It is provided by the invention Secondary coccus (Paracoccus versutus) D6 apt to change 16S rRNA gene orders are as shown in SEQ ID No.1.
It is a further object of the present invention to provide secondary coccus (Paracoccusversutus) D6CGMCC No.14119 apt to change Utilize high ammonia-nitrogen wastewater and fixed CO2The method for producing SCP, specific method comprise the following steps：
(1) prepared by seed liquor, and inoculation is cultivated into 15h under the conditions of seed culture medium, 30 DEG C.
(2) high ammonia-nitrogen wastewater is pre-processed, and high ammonia-nitrogen wastewater warp thread is filtered off and removes large granular impurity, and pH value is adjusted into 6~8.
(3) it is inoculated with, the inoculum concentration of seed liquor is the 8% of high ammonia-nitrogen wastewater volume.
(4) secondary coccus (Paracoccusversutus) D6 heterotrophic growths production SCP apt to change：Under aerobic condition, the present invention Bacterial strain secondary coccus (Paracoccusversutus) D6CGMCC No.14119 apt to change utilize high ammonia-nitrogen wastewater in organic matter and Ammonia nitrogen produces SCP, and it is 30 DEG C to control cultivation temperature, mixing speed is more than 200r/min, incubation time is 3~8 days.
(5) secondary coccus (Paracoccusversutus) D6 autophyting growths apt to change：When the solubility in high ammonia-nitrogen wastewater has When machine matter concentration drops to 1000mg/L (in terms of soluble chemical oxygen demand SCOD), lead to H2、O2And CO2Gaseous mixture (gas ratio Example is 7:2:1) it is respectively 30 DEG C and more than 200r/min, to keep cultivation temperature and mixing speed, and incubation time is 3~5 days.
(6) recovery of single cell protein, by secondary coccus (Paracoccusversutus) D6 heterotrophism apt to change and autophyting growth Bacterium solution separation of solid and liquid afterwards, thalline drying and processing obtain product single cell protein (SCP).
Described high ammonia-nitrogen wastewater includes but is not limited to breeding wastewater, biogas slurry, pharmacy waste water, food processing wastewater etc..
Described CO2Source includes but is not limited to industrial waste gas, biogas etc..
The advantage of the invention is that：
(1) secondary coccus apt to change of the present invention has unique metabolic function, can utilize high ammonia nitrogen during its chemoheterotrophy The elements such as the Organic carbon and nitrogen in waste water are grown, so as to realize high ammonia-nitrogen wastewater COD and ammonia nitrogen reduction；During chemautotrophy It is that carbon source is grown using carbon dioxide, realizes CO2Fixation and utilization.
(2) present invention can utilize CO2For carbon source, at the same consume the organic matter in livestock and poultry feces biogas slurry, a large amount of ammonia nitrogens and Trace element, then alleviate " greenhouse effects ", and a kind of processing mode pioneering and inventing, valuable is provided for biogas slurry.
(3) secondary coccus apt to change of the present invention has certain treatment effect to high ammonia-nitrogen wastewater, and COD is gone after inoculated and cultured Except rate is more than 65%, ammonia nitrogen removal frank is more than 68%.
(4) secondary coccus apt to change of the present invention has deodorization to high ammonia-nitrogen wastewater, because high ammonia-nitrogen wastewater is usual With pungent odour, single cell protein is produced by secondary coccus (Paracoccusversutus) D6 apt to change heterotrophism and autophyting growth White utilization of the process to ammonia nitrogen, reduces ammonia nitrogen concentration in waste water, reduces the volatilization of ammonia, so as to effectively remove the stench of waste water.
(5) secondary coccus apt to change of the present invention can utilize single cell protein of the high ammonia-nitrogen wastewater production with economic value In vain, its protein content accounts for the 60%~74% of dry cell weight.
Brief description of the drawings
Fig. 1 is the cellular morphologies of the present invention secondary coccus D6 apt to change under a scanning electron microscope；
The 96 orifice plates colour developing result that Fig. 2 is the present invention secondary coccus D6 apt to change；
Fig. 3 is growth curves of the present invention secondary coccus D6 apt to change under autotrophic condition；
Growth conditions optimizes column diagram, including different initial pH optimizations posts when Fig. 4 is secondary coccus D6 autotrophys apt to change of the invention Shape figure (a), different temperatures optimization column diagram (b), different vaccination amount optimization column diagram (c)；
Fig. 5 is the inventive method flow chart；
Fig. 6 is product single cell protein preparation process figure of the present invention.
Embodiment
Describe the present invention in detail with reference to embodiments.Embodiment is convenient to be better understood from the present invention, not to this The limitation of invention.By experimental science in itself put into practice characteristic and experimental period, energy, cost are limited, it is impossible to all checking With the interchangeable ins and outs of embodiment.But those skilled in the art can further be sieved on the basis of the present invention is understood Choosing and equivalent substitution obtain the technology changed, and it all forms the infringement to the technology of the present invention.
The culture medium prescription being related in embodiment is：
(1) seed culture medium, for Heterotrophic culture, it is formulated and is：Peptone 10g, beef extract 5g, NaCl 5g, adds distilled water To 1000mL (solid medium adds agar 20g).
(2) minimal medium, for autotrophy culture, it is formulated and is：NH4HCO33g, KH2PO41g, K2HPO4 2g,NaCl 2g, CaCl20.01g, MgSO4·7H2O 0.2g, FeSO4·7H2O 0.01g, micro- 2mL, add distilled water to 1000mL (solid medium adds agar 20g), wherein micro- component (mg/L)：ZnSO4·7H2O 1.0, MoO31.0 MnSO4·5H2O 1.0, H3BO30.4, CuSO4·5H2O 7.0, CoCl2·6H2O 1.0,NiSO4·7H2O 1.0。
Embodiment 1：The screening of hydroxide bacterium
Chengdu dragon's fountain post soybean rhizosphere 5g is taken to fill H into the anaerobism bottle equipped with 150mL minimal mediums2、 O2And CO2Gaseous mixture (molar ratio 7:2:1, be mol ratio in full), 150r/min shaking tables under the conditions of being 7,30 DEG C in pH Culture.Again inflate every 24h, 48h changes culture medium, transferred after gas using after tending towards stability with 2% inoculum concentration.So Cultivate 40 days repeatedly, bacterium solution gradient dilution (10 is taken after gas is using stabilization-2~10-7) it is inoculated in rolling pipe minimal medium In, 30 DEG C of shaking table culture 48h after inflation.Liquid inorganic medium culture is fallen within elbow capillary picking single bacterium, then uses flat board Method of scoring separation single bacterium colony three times more than, until colonial morphology is consistent, last microscopy must purify strain.
Embodiment 2：The identification of hydroxide bacterium
The bacterial strain isolated and purified is observed under SEM (SEM), as a result as shown in Figure 1：The bacterium is short It is shaft-like, long 0.6~1.1 μm, atrichia；Gram's staining identifies the bacterium for feminine gender.
Using bacterium full-length genome Rapid extraction kit, the full-length genome of pure bacterial strain is extracted, by from bacterial 16 S RRNA universal primers 27F and 1492R enter performing PCR amplification, then sequencing analysis.Sequencing result is through the BLAST in ncbi database Compare, identify that the bacterial strain and the homology of secondary coccus (Paracoccus versutus) apt to change are 99%, be secondary coccus apt to change (Paracoccus versutus), secondary coccus D6 apt to change is named as, is the preservation strain secondary coccus apt to change of the present invention D6CGMCC No.14119。
Physiology and biochemistry identification is carried out to secondary coccus D6 apt to change, 94 are carried out to bacterial strain using the MicroPlate of BiologGen III Kind biochemical test test, reads the phenotype collection of illustrative plates showed on the microwell plates of Gen III to identify bacterium by software, The 96 orifice plates colour developing result that accompanying drawing 2 is the secondary coccus D6CGMCC No.14119 apt to change of the present invention.As a result show that the bacterial strain does good Become secondary coccus (Paracoccusversutus).
Growth curve research is carried out to secondary coccus D6 apt to change, from minimal medium, and fills H2、O2And CO2Gaseous mixture (ratio 7:2:1) autotrophy culture is carried out, as a result as shown in Figure 3：Secondary coccus D6 apt to change can be sharp in minimal medium Use CO2Growth, after cultivating 4.5d, bacterium solution OD values reach 2.889.
Embodiment 3：Secondary coccus D6 autophyting growths condition optimizing experiment apt to change
Method and step：Prepare minimal medium first to be sub-packed in 250mL anaerobism bottles, butyl rubber plug sealing, at 121 DEG C Sterilize 20min, using displacement degassing method distribution：H is connected with emulsion tube2Gas cylinder, and syringe needle is connected in the other end of emulsion tube, will This syringe needle is inserted in anaerobism bottle by plug, then another syringe needle is inserted into anaerobism bottle by plug, to be vented, then by gaseous mixture Ratio (H2：O2：CO2=7：2：1) O of respective amount is squeezed into syringe2And CO2.Then the seed inoculation liquid of bacterial strain of the present invention is connect Kind utilizes air gauge to monitor gas utilization power, batch (-type) make-up gas in completely charged anaerobism bottle.Tried using single factor test Test the influence for investigating different pH, different temperatures and different vaccination amounts to thalli growth and production SCP.Shaking flask initial fermentation condition is： 2% inoculum concentration, pH7,30 DEG C, 200r/min shaking table cultures 6d.
(a) different initial pH optimization：Configure the inorganic salts culture that initial pH is respectively 5.0,6.0,7.0,8.0,9.0 Base, other conditions are initial fermentation condition, and its dry cell weight (DCW) is determined after shaking table culture 6d and SCP accounts for dry weight content.As a result As shown in accompanying drawing 4 (a), when initial pH is 7, DCW and SCP content highests, respectively 3.3g/L and 72.3%, therefore bacterial strain of the present invention The initial pH of optimum growh is 7.
(b) optimization of different temperatures：Other conditions are initial fermentation condition, be respectively placed in temperature for 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C of shaking table shaken cultivation cultures, determine its dry cell weight (CDW) after 6d and SCP account for dry weight content.Measurement result As shown in accompanying drawing 4 (b), when temperature is 30 DEG C, DCW and SCP content highests, respectively 3.9g/L and 79.6%, thus it is of the invention Bacterial strain optimum growth temp is 30 DEG C.
(c) optimization of different vaccination amount：Other conditions are initial fermentation condition, adjustment inoculum concentration is respectively 2%, 4%, 6%th, its dry cell weight (CDW) is determined after 8%, 10%, shaking table culture 6d and SCP accounts for dry weight content.Measurement result such as accompanying drawing 4 (c) shown in, when inoculum concentration is 8%, DCW and SCP content highests, respectively 3.7g/L and 79%, therefore selection inoculum concentration is for 8% Optimum condition.
Embodiment 4：Secondary coccus D6 apt to change removes the effect test of stink using meat products processing waste water production SCP simultaneously
Using meat products processing waste water as raw material, the large granular impurity removed in waste water is filtered off with yarn, pH value of waste water is adjusted to 6 simultaneously It is sub-packed in 500mL anaerobism bottles (liquid amount 150mL), in 121 DEG C of autoclaving 20min.Bacterium will be aseptically inoculated with Liquid is added in anaerobism bottle with 12mL amount, puts shaking table shaken cultivation (30 DEG C, 200r/min).
To be inoculated with other secondary coccus Z7 apt to change that this laboratory screening obtains as control group, inoculum concentration 8%, put shaking table and shake Swing culture (30 DEG C, 200r/min) 3 days.
Not connect the corresponding waste water of bacterium as blank group, Setup Experiments three are parallel.Determined after Heterotrophic culture 3d bacterium solution SCOD, The ammonia nitrogen concentration of centrifuged supernatant, DCW and SCP contents, take its average value.Measurement result is as shown in table 1 below, and it is kind to be inoculated with the present invention Become secondary coccus D6 waste water COD after 3d clearance as 78.1%, ammonia nitrogen removal frank 68%, and caused dry cell weight is 4.5g/L, wherein SCP contents are 68%.
The bacterium also has remarkable effect in terms of deodorization, and the odor strength and ammonia concentration of meat products processing waste water have substantially Decline.
The D6 of table 1 utilizes meat products processing waste water production SCP situations
Embodiment 5：Secondary coccus D6 apt to change removes the effect test of stink using piggery wastewater production SCP simultaneously
Using piggery wastewater as raw material, the large granular impurity in waste water is removed with filtered through gauze, pH value of waste water is adjusted to 7 and divided In 500mL anaerobism bottles (liquid amount 150mL), in 121 DEG C of autoclaving 20min.Bacterium solution will be aseptically inoculated with It is added to 12mL amount in anaerobism bottle, puts shaking table shaken cultivation (30 DEG C, 200r/min).Bacterium solution SCOD is determined every three days, When the SCOD in fermentation to 5d, waste water drops to below 1000mg/L, lead to H2、O2And CO2Gaseous mixture (gas ratio 7: 2:1) gas utilization power, batch (-type) supplement mixed gas, are monitored using air gauge.
To be inoculated with other secondary coccus Z7 apt to change that this laboratory screening obtains as control group, ibid identical culture 8 days.
Not connect the corresponding waste water of bacterium as blank control, Setup Experiments three are parallel.Cultivate determine after 8d bacterium solution SCOD, from The ammonia nitrogen concentration of supernatant, DCW and SCP contents, take its average value.Measurement result is as shown in table 2 below, and the inoculation present invention is apt to change Secondary coccus D6 piggery wastewater COD after 8d clearance is 65%, ammonia nitrogen removal frank 78%, and caused dry cell weight is 5.2g/L, wherein SCP contents are 60.5%, and CO2Fixed efficiency be 559.8mg/ (Lday).
The bacterium also has a remarkable effect in terms of deodorization, the odor strength and ammonia concentration of piggery wastewater have it is obvious under Drop.
The D6 of table 2 utilizes piggery wastewater production SCP situations
Embodiment 6：Secondary coccus D6 apt to change removes the effect test of stink using chicken manure biogas slurry production SCP simultaneously
Using chicken manure biogas slurry as raw material, the large granular impurity removed in biogas slurry is filtered off with yarn, then pH value is adjusted to 8 and is sub-packed in In 500mL anaerobism bottles (liquid amount 150mL), in 121 DEG C of autoclaving 20min.Aseptically will inoculation bacterium solution with 12mL amount is added in anaerobism bottle, puts shaking table shaken cultivation (30 DEG C, 200r/min).Bacterium solution SCOD is determined every three days, when Fermentation is to 8d, when the SCOD in biogas slurry drops to below 1000mg/L, leads to H2、O2And CO2Gaseous mixture (gas ratio 7:2: 1) gas utilization power, batch (-type) supplement mixed gas, are monitored using air gauge.
To be inoculated with other secondary coccus Z7 apt to change that this laboratory screening obtains as control group, ibid identical culture 13 days.
Not connect the corresponding biogas slurry of bacterium as blank control, Setup Experiments three are parallel.Cultivate determine after 13d bacterium solution SCOD, The ammonia nitrogen concentration of centrifuged supernatant, DCW and SCP contents, take its average value.Measurement result is as shown in table 3 below, and it is kind to be inoculated with the present invention Become secondary coccus D6 chicken manure biogas slurry COD after 13d clearance as 92.5%, ammonia nitrogen removal frank 93.5%, caused thalline Dry weight is 12.95g/L, and wherein SCP contents are 74%, and CO2Fixed efficiency be 722.8mg/ (Lday).
The bacterium also has a remarkable effect in terms of deodorization, the odor strength and ammonia concentration of chicken manure biogas slurry have it is obvious under Drop.Table 3D6 utilizes chicken manure biogas slurry production SCP situations
Embodiment 7：Separation and Extraction SCP
On the basis of embodiment 6, bacterium solution is centrifuged into (5000r/min, 30min), drying and processing (60 DEG C, 20h) produces To product SCP 1.44g, product has obvious feed fragrance.Product such as accompanying drawing 6.
Sequence table
<110>Shenzhen Ke Gerui environment friendly biologicals Science and Technology Ltd.
<120>One plant of secondary coccus apt to change and the method for high ammonia-nitrogen wastewater processing by-product single cell protein
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<170> SIPOSequenceListing 1.0
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gtaacgcgtg ggaatatgcc ctttggtacg gaatagtcct gggaaactgg gggtaatacc 120
gtatgcgccc ttcgggggaa agatttatcg ccaaaggatt agcccgcgtt ggattaggta 180
gttggtgggg taatggccta ccaagccgac gatccatagc tggtttgaga ggatgatcag 240
ccacactggg actgagacac ggcccagact cctacgggag gcagcagtgg ggaatcttag 300
acaatggggg caaccctgat ctagccatgc cgcgtgagtg atgaaggccc tagggttgta 360
aagctctttc agctgggaag ataatgacgg taccagcaga agaagccccg gctaactccg 420
tgccagcagc cgcggtaata cggagggggc tagcgttgtt cggaattact gggcgtaaag 480
cgcacgtagg cggaccggaa agttgggggt gaaatcccgg ggctcaaccc cggaactgcc 540
ttcaaaacta tcggtctgga gttcgagaga ggtgagtgga attccgagtg tagaggtgaa 600
attcgtagat attcggagga acaccagtgg cgaaggcggc tcactggctc gatactgacg 660
ctgaggtgcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa 720
acgatgaatg ccagtcgtcg ggcagcatgc tgttcggtga cacacctaac ggattaagca 780
ttccgcctgg ggagtacggt cgcaagatta aaactcaaag gaattgacgg gggcccgcac 840
aagcggtgga gcatgtggtt taattcgaag caacgcgcag aaccttacca acccttgaca 900
tcccaggacc ggcccggaga cgggtctttc acttcggtga cctggagaca ggtgctgcat 960
ggctgtcgtc agctcgtgtc gtgagatgtt cggttaagtc cggcaacgag cgcaacccac 1020
acttccagtt gccatcattt ggttgggcac tctggaagaa ctgccgatga taagtcggag 1080
gaaggtgtgg atgacgtcaa gtcctcatgg cccttacggg ttgggctaca cacgtgctac 1140
aatggtggtg acagtgggtt aatccccaaa agccatctca gttcggattg gggtctgcaa 1200
ctcgacccca tgaagttgga atcgctagta atcgcggaac agcatgccgc ggtgaatacg 1260
ttcccgggcc ttgtacacac cgcccgtcac accatgggag ttgggtctac ccgacggccg 1320
tgcgctaacc agcaatgggg cagcgaccac gtagcccgcg gct 1363

Claims (10)

1. one plant of secondary coccus (Paracoccus versutus) D6 apt to change, China Microbiological was preserved on 05 12nd, 2017 Culture presevation conservator's common micro-organisms center, deposit number are：CGMCC No.14119.
2. secondary coccus D6CGMCC No.14119 apt to change described in claim 1, it is characterised in that：Physiological characteristic is：Cell is short Shaft-like, long 0.5~1.1 μm, Gram-negative, atrichia；Bacterium colony is rounded, and rat, the smooth of the edge is neat, and color is not Transparent milky；It is 5.0~9.0 to grow pH；Growth temperature is 25~35 DEG C；The bacterium is amphitrophy type, is distinguished during heterotrophism Grown using the organic carbon in high ammonia-nitrogen wastewater and ammonia nitrogen as carbon source, nitrogen source, respectively with carbon dioxide and high ammonia nitrogen during autotrophy Ammonia nitrogen in waste water is carbon source, nitrogen source is grown, and the bacterium can utilize the ammonia nitrogen production in waste water under heterotrophism, autotrophic condition Single cell protein.
3. secondary coccus D6CGMCC No.14119 apt to change described in claim 1,16S rRNA gene orders such as SEQ ID No.1 It is shown.
4. the method for secondary coccus D6CGMCC No.14119 processing high ammonia-nitrogen wastewater apt to change, specific method bag described in claim 1 Include following steps：
(1) prepared by seed liquor, by inoculation in seed culture medium；
(2) high ammonia-nitrogen wastewater pre-processes, and high ammonia-nitrogen wastewater warp thread filters off and removes large granular impurity；
(3) it is inoculated with, the inoculum concentration of seed liquor is the 2~8% of high ammonia-nitrogen wastewater volume；
(4) secondary coccus D6 heterotrophic growths production SCP apt to change：Under aerobic condition, bacterial strain secondary coccus D6CGMCC apt to change of the present invention No.14119 utilizes organic matter and ammonia nitrogen production SCP in high ammonia-nitrogen wastewater, and to control cultivation temperature be 30 DEG C, mixing speed is More than 200r/min, incubation time are 3~8 days；
(5) secondary coccus D6 autophyting growths apt to change：When the SOM concentration in high ammonia-nitrogen wastewater drops to 1000mg/L, Logical H2, O2 and CO2 gaseous mixture, gas molar ratio are 7:2:1, keep cultivation temperature and mixing speed be respectively 30 DEG C and More than 200r/min, incubation time are 3~5 days.
5. the method for high ammonia-nitrogen wastewater is handled according to claim 4, it is characterised in that：By secondary coccus D6 apt to change through heterotrophism and Bacterium solution separation of solid and liquid, thalline drying and processing after autophyting growth obtain product single cell protein.
6. according to the method for the processing high ammonia-nitrogen wastewater of claim 4 or 5, it is characterised in that：Described high ammonia-nitrogen wastewater is Breeding wastewater, biogas slurry, pharmacy waste water, one kind of food processing wastewater.
7. according to the method for the processing high ammonia-nitrogen wastewater of claim 4 or 5, it is characterised in that：Described CO2Source is industry Waste gas or biogas.
8. according to the method for the processing high ammonia-nitrogen wastewater of claim 4 or 5, it is characterised in that：The seed culture medium, formula For：Peptone 10g, beef extract 5g, NaCl 5g, add distilled water that fluid nutrient medium is made to 1000mL or add agar 20g to be made admittedly Body culture.
9. according to the method for the processing high ammonia-nitrogen wastewater of claim 4 or 5, it is characterised in that：In the step (1), bacterial strain On seed culture medium 15h is cultivated under the conditions of 30 DEG C.
10. according to the method for the processing high ammonia-nitrogen wastewater of claim 4 or 5, it is characterised in that：In the step (2), filtering High ammonia-nitrogen wastewater pH value afterwards is adjusted to 6~8.
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CN110184205A (en) * 2018-02-23 2019-08-30 河北科技大学 A kind of fermentation process of penicillin degradation bacteria strains
CN110655198A (en) * 2018-06-29 2020-01-07 龙岩学院 Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain
CN110656057A (en) * 2018-06-29 2020-01-07 龙岩学院 Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed liquid, preparation method and application thereof
CN111893079A (en) * 2020-09-03 2020-11-06 广州希奕餐厨降解设备有限公司 Compound microbial inoculum for vegetable market garbage compost as well as preparation method and application thereof
CN114058553A (en) * 2021-12-10 2022-02-18 青岛蔚蓝赛德生物科技有限公司 Composite microbial inoculum for degrading COD in wastewater and preparation method and application thereof
CN114214229A (en) * 2021-12-08 2022-03-22 中国科学院天津工业生物技术研究所 Paracoccus panthagi strain MA3, production method and application thereof
CN114717141A (en) * 2022-03-04 2022-07-08 浙江台州秀川科技有限公司 Complex flora for treating wastewater containing gallic acid and application thereof

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CN110184205A (en) * 2018-02-23 2019-08-30 河北科技大学 A kind of fermentation process of penicillin degradation bacteria strains
CN110184205B (en) * 2018-02-23 2022-09-27 河北科技大学 Fermentation method of penicillin degradation strain
CN110655198A (en) * 2018-06-29 2020-01-07 龙岩学院 Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain
CN110656057A (en) * 2018-06-29 2020-01-07 龙岩学院 Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed liquid, preparation method and application thereof
CN110655198B (en) * 2018-06-29 2022-01-28 龙岩学院 Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain
CN110656057B (en) * 2018-06-29 2022-08-09 龙岩学院 Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed liquid, preparation method and application thereof
CN111893079A (en) * 2020-09-03 2020-11-06 广州希奕餐厨降解设备有限公司 Compound microbial inoculum for vegetable market garbage compost as well as preparation method and application thereof
CN114214229A (en) * 2021-12-08 2022-03-22 中国科学院天津工业生物技术研究所 Paracoccus panthagi strain MA3, production method and application thereof
CN114214229B (en) * 2021-12-08 2023-04-18 中国科学院天津工业生物技术研究所 Paracoccus pantoea strain MA3, production method and application thereof
CN114058553A (en) * 2021-12-10 2022-02-18 青岛蔚蓝赛德生物科技有限公司 Composite microbial inoculum for degrading COD in wastewater and preparation method and application thereof
CN114058553B (en) * 2021-12-10 2023-06-23 青岛蔚蓝赛德生物科技有限公司 Composite microbial agent for degrading COD in wastewater as well as preparation method and application thereof
CN114717141A (en) * 2022-03-04 2022-07-08 浙江台州秀川科技有限公司 Complex flora for treating wastewater containing gallic acid and application thereof

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