CN110452900A - A kind of preparation method for the compound fixation support of PVA-SA embedding degrading microorganism - Google Patents
A kind of preparation method for the compound fixation support of PVA-SA embedding degrading microorganism Download PDFInfo
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Abstract
A kind of preparation method for the compound fixation support of PVA-SA embedding degrading microorganism: it is characterized in that the described method comprises the following steps: a) aseptically, the bacteria suspension of degrading microorganism and the composite material solution of sterilization treatment being mixed；By the polyvinyl alcohol of 95~105g/L, the sodium alginate of 9~11g/L, the SiO of 28~32g/L in the composite material solution2, the CaCO of 4.5~5.5g/L3, the active carbon of 5~7g/L and the deionized water of surplus be formulated；B) the composite material solution for being uniformly mixed with bacteria suspension in an aseptic environment obtaining step 1) instills in crosslinking agent dropwise, obtain fixation support after cure process, the crosslinking agent be saturated dissolution mass fraction is 1.8~2.2% in boric acid solution calcium chloride solid and with sodium hydroxide solution tune pH be 6.1~6.3 after obtain.
Description
Technical field
The present invention relates to a kind of imbedded microbe fixation support of biological treatment for coking wastewater and its preparation sides
Method.
Background technique
Coking wastewater is the waste water generated in coke refining, gas purification and coking removal process, it includes many multiple
The gas chromatographies such as miscellaneous organic pollutant, including quinolines, pyridine, phenols, benzene class, are listed in difficult sewage.Due to more
Have the effects that sum it up (Additive), collaboration (Synergism), antagonism (Antagonism) between kind pollutant, using normal
When advising bioanalysis progress wastewater treatment, coking wastewater is more toxic microorganism growth, leads to treating coking wastewater biologically
When, there are the pollutant concentration of tolerance is low, degradation efficiency is poor, and the problems such as activated sludge easy in inactivation have seriously affected use
The application of this measure of Biochemical method combined pollution coking wastewater.
Embedded immobilization microbial technology is the research emphasis of current water treatment field, using consolidating for embedding degrading microorganism
Surely change carrier, treatment effect when can be improved using microbiological treatment coking wastewater overcomes that microbial cell is too small and water
Solution separation is more difficult, easily causes the drawbacks such as secondary pollution, and so that intracellular enzyme system is saved, complete, reaction is more abundant.
The microcosmic bracket of appropriate and fine immobilization can assign immobilized cell certain characteristic, have absorption and biodegradation character
Fixation support microballoon, target contaminant can be made to be adsorbed on carrier surface to promoting degradation bacteria strains to come into full contact with pollution
Object, accelerated degradation process.But under study for action it was found that when degrading microorganism is the specific composite flora screened, often
It rule imbedded microbe and prepares fixation support mode there are person's adverse effect to degradation effect, therefore providing one kind can be abundant
Play the preparation side of the compound fixation support of PVA-SA of the embedding degrading microorganism of degrading microorganism processing coking wastewater effect
Method becomes urgent problem to be solved in the prior art.
Summary of the invention
To solve the above problems, the present invention is filtering out a kind of screening phenol, pyridine, quinoline efficient degradation flora basis
On, by optimizing the preparation process of fixation support, degrading microorganism processing coking wastewater can be given full play to by having obtained one kind
The fixation support preparation method of the embedding degrading microorganism of ability and the fixation support prepared using this method.
To solve the above problems, technical solution provided by the invention are as follows:
A kind of preparation method for the compound fixation support of PVA-SA embedding degrading microorganism: it is characterized in that the method packet
Include following steps:
A) aseptically, the bacteria suspension of degrading microorganism and the composite material solution of sterilization treatment are mixed；It is described
By the polyvinyl alcohol of 95~105g/L, the sodium alginate of 9~11g/L in composite material solution, the silica of 28~32g/L,
The deionized water of the calcium carbonate of 4.5~5.5g/L, the active carbon of 5~7g/L and surplus is formulated.
B) the composite material solution for being uniformly mixed with bacteria suspension in an aseptic environment obtaining step 1) instills friendship dropwise
Join in agent, fixation support is obtained after cure process, the crosslinking agent is that dissolution mass fraction is 1.8 in saturation boric acid solution
~2.2% calcium chloride solid and with sodium hydroxide solution tune pH be 6.1~6.3 after obtain；
The degrading microorganism is screened using following methods
1) sample: acquisition has the wastewater sample of sludge from apparatus for treating carbonized waste water, includes in the wastewater sample
Aerobic treatment device water outlet and anaerobic processing device water outlet；
2) cultivate and switching: by sample be added containing phenol, pyridine, quinoline liquid inorganic salt culture medium in, in 27~
It is cultivated under the conditions of 33 DEG C, detects its degradation effect with UV scanning, as culture terminal when scanning curve drop is flat, with same
Sample condition of culture is repeatedly transferred, the inoculation for the efficient degradation flora for taking the supernatant after repeatedly transferring to obtain as screening
Bacterium, the liquid inorganic salt culture medium proportion are as follows: NaCl 0.9~1.1g/L, NH4NO30.9~1.1g/L, K2HPO4
1.20~1.8g/L, KH2PO40.4~0.6g/L, MgSO4·7H2O 0.15~0.25g/L, pH 7.0-7.2, phenol 10~
100mg/L, 10~100mg/L of pyridine, 10~100mg/L of quinoline and surplus distilled water, be only with phenol, pyridine, quinoline
One carbon nitrogen source；
3) isolate and purify: using LB solid medium to the degradation flora that step 3) obtains be coated separation, repeatedly instead
Multiple purifying, and conservation.
The preparation method of the compound fixation support of PVA-SA of a kind of embedding degrading microorganism, it is characterized in that described
The proportion of liquid inorganic salt culture medium is preferably NaCl 1.0g/L, NH4NO31.0g/L, K2HPO41.5g/L, KH2PO4
0.5g/L, MgSO4·7H2O 0.2g/L, 40~60mg/L of phenol, 40~60mg/L of pyridine, 40~60mg/L of quinoline cultivate item
Part is that culture medium is placed in triangular flask, in 30 DEG C, under the conditions of 180rpm, is cultivated.
The preparation method of the compound fixation support of PVA-SA of a kind of embedding degrading microorganism, it is characterized in that described
Flora mainly includes quantity than for 95~105:25~30:210~230:55~65:90~100 Rhodococcus ruber
(Rhodococcus ruber), medicinal clostridium botulinum (Patulibacter medicamentivorans), locust section pair coccus
(Paracoccus acridae), cornea loosen microbacterium (Microbacterium keratanolyticum) and celebrating the red ball of sheng, a reed pipe wind instrument
Bacterium (Rhodococcus qingshengii).
The preparation method of the compound fixation support of PVA-SA of a kind of embedding degrading microorganism, it is characterized in that described
Bacteria suspension is transferred in LB liquid medium the preparation method comprises the following steps: bacterium will be inoculated with, and 30 DEG C, 180rmp environment culture 48h are planted
Above-mentioned 1mL seed liquor is inoculated into the LB culture medium of 100mL by sub- liquid, 180r/min, 30 DEG C culture for 24 hours, 6000r/min, 4
DEG C centrifugation 10min, discard supernatant, with sterile water blow and beat be resuspended, centrifugation, abandon supernatant, be so repeated 3 times, by obtained thallus use
Inorganic salt solution is prepared into OD600For 2.0 bacteria suspension, the inorganic salt content of the inorganic salt solution is NaCl 1.0g/L,
NH4NO31.0g/L, K2HPO41.5g/L, KH2PO40.5g/L, MgSO4·7H2O 0.2g/L, 1mL microelement stock solution/
L, the ingredient of the microelement stock solution are KI 0.005g, MnSO4·4H2O 0.2g, CuSO4·2H2O 0.02g,
ZnSO4·7H2O 0.2g, Na2MoO4·2H2O 0.25g, H3BO30.008g, FeCL36H2O 0.1g, adds water to 100mL；
By the polyvinyl alcohol of 100g/L, the sodium alginate of 10g/L, the SiO of 30g/L in the composite material solution2, the CaCO of 5g/L3、
The active carbon of 6g/L and the deionized water of surplus are formulated, and the crosslinking agent is by dissolving mass fraction in saturation boric acid solution
2% calcium chloride solid and with the sodium hydroxide tune pH of 0.5mol/L be 6.22 after obtain, the cure process condition be at 4 DEG C
Under the conditions of crosslinking Treatment 12~for 24 hours, the volume ratio of the bacteria suspension and composite material solution is 5~30:100, preferably 18~22:
100。
When composite solution instills in crosslinking agent dropwise, droplet size is controlled, makes the compound fixation support obtained
The diameter of bead is 0.3~0.7cm, preferably 0.4~0.6cm.
The present invention also provides the systems of the compound fixation support of PVA-SA using any embedding degrading microorganism
The fixation support that Preparation Method obtains.
Compared with prior art, the beneficial effects of the present invention are:
1. a kind of preparation side of the compound fixation support of PVA-SA of embedding degrading microorganism provided by the invention
Method, easy to operate, low for equipment requirements and cheap, manufacturing process is comparatively safe.
2. the present invention passes through the raw material proportioning and preparation process of preferred composite materials solution, obtained fixation support, benefit
With the absorption property of active carbon in raw material, adsorbs pollutant and accelerate biodegrade, the fixation support of preferred method production is small
Ball has the ability for complicated pollutant of preferably degrading.
3. the fixation support for using preparation method of the present invention to obtain provides wide space for microorganism growth, be conducive to
The growth and breeding of microorganism and removal to polluter increase biodegrade before the application in environment remediation field
Scape.
4. producing principle of the invention is simple, reasonable in design, immobilized microorganism the small ball's diameter obtained is in 0.5cm
Left and right, percent opening is high, and mass-transfer performance is good, and biological affinity is strong, the pollution amelioration of the better application environment of energy.Operating cost is low,
It is easy to operate, it is generally applicable to field of environment pollution control, there is very high practical application value, use easy to spread.
Detailed description of the invention
Fig. 1 is the internal microstructure shape appearance figure of the fixation support bead of 4 method of embodiment preparation；
Fig. 2 is the surface microstructure shape appearance figure of the fixation support bead of 4 method of embodiment preparation.
Specific embodiment
Further description of the technical solution of the present invention With reference to embodiment.
Embodiment 1: the method for screening phenol, pyridine, quinoline efficient degradation flora
It the described method comprises the following steps:
1) it samples
Acquisition has the coking wastewater of sludge, including 1mL from the sewage-treatment plant of Tangshan Da Feng coking Co., Ltd
Raw water (band mud), 1mL anaerobic pond water outlet (band mud), 1mL aerobic tank (band mud), 1mL secondary clarifier effluent (band mud).A
2) culture and switching
The coking wastewater of each process point acquisition of step 1) acquisition is added together in 100mL liquid inorganic salt culture medium,
The liquid inorganic salt culture medium proportion is NaCl 1.0g/L, NH4NO31.0g/L, K2HPO41.5g/L, KH2PO4 0.5g/
L, MgSO4·7H2O 0.2g/L, phenol 50mg/L, pyridine 50mg/L, quinoline 50mg/L.Triangle bottleneck is placed in seal with sealed membrane
(sealing film surface have air hole) is placed on 30 DEG C, cultivates in 180rmp shaking table.Its degradation is detected with ultraviolet full wavelength scanner
Effect, wherein phenol maximum absorption band is at 210nm and 270nm, and the maximum absorption band of quinoline is at 313nm and 225nm, pyridine
Maximum absorption band at 256nm, culture 3 days after scanning curve drop head up display show that phenol, pyridine, quinoline have been degraded
At, and in this, as culture terminal.After reaching culture terminal, switching supernatant is as inoculation bacterium solution, with same condition of culture
It carries out repeatedly switching and obtains efficient degradation flora.
3) it isolates and purifies:
Efficient degradation flora bacterium solution (the hereinafter referred to as enrichment bacterium obtained after repeatedly switching is separated using dilution plate rubbing method
Liquid).The sterile water of 10 pipe 9mL is arranged, by 10-1、10-2、10-3、10-4、10-5、10-6、10-7、10-8Number consecutively.In nothing
Under bacterium operating condition, draws 1mL enrichment bacterium solution and be placed in the first pipe 9mL sterile water and shake, as 10-1The bacterium solution of concentration.Together
Quadrat method, which successively dilutes, to be obtained to 10-8The bacterium solution of concentration.Respectively from 10-3、10-4、10-5、10-6、10-7、10-8Bacterium solution in respectively inhale
Take 100 μ L bacterium solutions on the plate with LB solid medium, rotary coating is uniform on plate with glass spreading rod, Mei Genong
The bacterium solution of degree makees 2 plates.The plate for being inoculated with microorganism is placed in 30 DEG C of constant incubator and is cultivated.The LB solid training
The ingredient for supporting base is peptone 10g, sodium chloride 10g, beef extract 5g, 20.0~25.0g of agar, distilled water 1000ml, pH=7.0
~7.2.After plate grows bacterium colony, chooses the preferable bacterium of growing way and purify in next step.With oese with one ring of sterile working picking
Bacterium colony is inoculated into the sterilizing flat board of brand-new, and selected bacterial strain is further purified.The purifying of bacterial strain uses plate streaking
Method, detailed process is as follows: first on one side work first time parallel scribing 3-4 item of the oese in brand-new plate for choosing bacterium colony, then
About 70 degree of angles of culture dish are rotated, and residue on oese is burnt up, are made second by first time dashed part after cooling
Parallel scribing, then third time is made by second of dashed part with same method and is crossed, it is overlapped front and back two lines.It will connect
It is cultivated in the constant incubator that kind has the plate of microorganism to be placed in 30 DEG C.The dominant strain being purified into is carried out using PCR method
Identification, the PCR method are the DNA for first extracting dominant strain, and the DNA of extraction is carried out PCR expansion using PCR amplification instrument later
Increase, then carry out the agarose gel electrophoresis of DNA, last genome 16SrDNA sequencing analysis determines kind.It is identified, screening
Obtained efficient degradation flora mainly includes Rhodococcus ruber (Rhodococcus ruber), medicinal clostridium botulinum
(Patulibacter medicamentivorans), locust section pair coccus (Paracoccus acridae), cornea are loosened microbot
Bacterium (Microbacterium keratanolyticum), celebrating sheng, a reed pipe wind instrument Rhodococcus sp (Rhodococcus qingshengii).Crimson ball
Bacterium (Rhodococcus ruber), medicinal clostridium botulinum (Patulibacter medicamentivorans), locust section pair coccus
(Paracoccus acridae), cornea loosen microbacterium (Microbacterium keratanolyticum), celebrating the red ball of sheng, a reed pipe wind instrument
The quantity ratio of bacterium (Rhodococcus qingshengii) is 100:27:218:58:96.
The preservation of strain :+750 μ L bacterium solution of 750 μ L glycerol mixes, -20 DEG C of preservations.
Embodiment 2~5: the preparation method of the compound fixation support of PVA-SA of kind embedding degrading microorganism.
The preparation of degradation flora seed liquor: the enrichment bacterium solution that embodiment 1 is obtained is transferred in LB liquid medium, and 30
DEG C, 180rmp environment culture 48h, obtain seed liquor.
The preparation of bacteria suspension: above-mentioned 1mL seed liquor is inoculated into the LB culture medium of 100mL, 180r/min, 30 DEG C of cultures
For 24 hours, 6000r/min, 4 DEG C of centrifugation 10min, discard supernatant, and are blown and beaten and are resuspended with sterile water, and centrifugation is abandoned supernatant, is so repeated 3 times,
By obtained thallus inorganic salt solution, (inorganic salt content of the inorganic salt solution is NaCl 1.0g/L, NH4NO3 1.0g/
L, K2HPO41.5g/L, KH2PO40.5g/L, MgSO4·7H2O 0.2g/L, 1mL microelement stock solution/L, the micro member
The ingredient of plain stock solution is KI 0.005g, MnSO4·4H2O 0.2g, CuSO4·2H2O 0.02g, ZnSO4·7H2O 0.2g,
Na2MoO4·2H2O 0.25g, H3BO30.008g, FeCl36H2O 0.1g, adds water to 100mL) it is prepared into OD600It is 2.0
Bacteria suspension.
Prepare immobilized microspheres:
The first step, the fixation support solution of preparation embedding flora.Weigh 10g polyvinyl alcohol and 1g sodium alginate in
It stirs evenly in 250mL beaker to entering 100mL deionized water, impregnates 28h at room temperature, soften it sufficiently.It is added later
3gSiO2、0.5gCaCO3With 0.5g active carbon, active carbon is multiple on a small quantity, quickly stirring prevents from agglomerating.Add in heat collecting type constant temperature
90 DEG C of water-bath dissolutions while stirring, constantly add water to keep mixed liquor volume constant during water-bath in pyromagnetic force blender.To poly-
Vinyl alcohol and sodium alginate are complete molten to obtain composite material solution.121 DEG C of sterilizing 30min in autoclave sterilization pot later.It will
It is mixed under bacteria suspension and composite material solution aseptic condition.The volume ratio of bacteria suspension and composite material solution is A:100.
Second step prepares crosslinking agent.The calcium chloride solid that dissolution mass fraction is 2% in saturation boric acid solution is used in combination
The sodium hydroxide tune pH of 0.5mol/L is 6.22.
Third step, the preparation of sodium alginate-polyvinyl alcohol complex microsphere.Under aseptic condition, by bacteria suspension and it is cooled to room
The complex carrier solution of temperature is uniformly mixed, and it is outstanding to be uniformly mixed with bacterium with the glass syringe absorption equipped with Glue dripping head of sterilization treatment
The composite material sol drop of liquid enters in crosslinking agent, to control the dynamics of manipulation, so that embedded material declines in droplets.
In 4 DEG C of crosslinking 12h.The fixation support bead of embedding flora is obtained, the bead after being crosslinked filters out, and rinses 1- with deionized water
3 times until the diameter range of finished product fixation support bead is 0.3~0.7cm without white solution generation.
The bacteria suspension of 2~embodiment of embodiment 5 see the table below with composite material solution proportion
| Number | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| A | 5 | 10 | 20 | 30 |
The internal microstructure pattern and surface microstructure shape of fixation support bead wherein are obtained to 4 method of embodiment
Looks (electron microscope) are as depicted in figs. 1 and 2 respectively.
The carrier microballoons analyzing of applying effects of immobilized high efficiency biodegradation bacterium group
2~embodiment of embodiment 5 prepare embedding flora fixation support bead, add respectively in containing phenol, pyridine,
In the inorganic salt liquid culture medium of three kinds of characteristic contaminations of quinoline.The initial mass concentration of three kinds of characteristic contaminations is 100mg/
L。
The additional amount of fixation support bead is 30g/100mL
Under the conditions of T=30 DEG C, r=180r/min timing sampling measurement phenol, three kinds of pollutants of pyridine and quinoline it is dense
Degree.Record the time of the degradable three kinds of pollutants of each embodiment fixation support bead
| Number | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| The degradable time/h | 24 | 18 | 11 | 15 |
It is seen from the above data that the fixation support bead of different bacteria suspensions and the preparation of composite material solution proportion,
Can three kinds of degradable phenol, pyridine, quinoline characteristic contaminations, but embodiment 4 prepare fixation support bead degrade speed
Degree is most fast, shows better treatment effect.
Claims (6)
1. a kind of preparation method for the compound fixation support of PVA-SA for embedding degrading microorganism: it is characterized in that the method includes
Following steps:
A) aseptically, the bacteria suspension of degrading microorganism and the composite material solution of sterilization treatment are mixed；It is described compound
By the polyvinyl alcohol of 95~105g/L, the sodium alginate of 9~11g/L, the SiO of 28~32g/L in material solution2, 4.5~
The CaCO of 5.5g/L3, the active carbon of 5~7g/L and the deionized water of surplus be formulated；
B) the composite material solution for being uniformly mixed with bacteria suspension in an aseptic environment obtaining step 1) instills crosslinking agent dropwise
In, obtain fixation support after cure process, the crosslinking agent be in saturation boric acid solution dissolution mass fraction be 1.8~
2.2% calcium chloride solid and with sodium hydroxide solution tune pH be 6.1~6.3 after obtain；
The degrading microorganism is screened using following methods:
1) sample: acquisition has the wastewater sample of sludge from apparatus for treating carbonized waste water, includes aerobic in the wastewater sample
Processing unit water outlet and anaerobic processing device water outlet；
2) cultivate and switching: by sample be added containing phenol, pyridine, quinoline liquid inorganic salt culture medium in, in 27~33 DEG C
Under the conditions of cultivate, detect its degradation effect with UV scanning, when scanning curve drop it is flat when as culture terminal, equally to train
The condition of supporting repeatedly is transferred, the inoculation bacterium for the efficient degradation flora for taking the supernatant after repeatedly transferring to obtain as screening,
The liquid inorganic salt culture medium proportion is as follows: NaCl 0.9~1.1g/L, NH4NO30.9~1.1g/L, K2HPO41.20~
1.8g/L, KH2PO40.4~0.6g/L, MgSO4·7H2O 0.15~0.25g/L, pH 7.0-7.2,10~100mg/ of phenol
L, the distilled water of 10~100mg/L of pyridine, 10~100mg/L of quinoline and surplus, using phenol, pyridine, quinoline as sole carbon nitrogen
Source；
3) isolate and purify: using LB solid medium to the degradation flora that step 2) obtains be coated separation, repeatedly cross it is pure
Change, and conservation.
2. a kind of preparation method of compound fixation support of PVA-SA for embedding degrading microorganism as described in claim 1,
The proportion for being characterized in the liquid inorganic salt culture medium is preferably NaCl 1.0g/L, NH4NO31.0g/L, K2HPO41.5g/L
KH2PO40.5g/L, MgSO4·7H2O 0.2g/L, 40~60mg/L of phenol, 40~60mg/L of pyridine, 40~60mg/L of quinoline,
Condition of culture is that culture medium is placed in triangular flask, in 30 DEG C, under the conditions of 180rpm, is cultivated.
3. a kind of preparation method of compound fixation support of PVA-SA for embedding degrading microorganism as claimed in claim 1 or 2,
It is characterized in that the flora mainly include quantity than for 95~105:25~30:210~230:55~65:90~100 it is crimson
Coccus (Rhodococcus ruber), medicinal clostridium botulinum (Patulibacter medicamentivorans), locust section pair ball
Bacterium (Paracoccus acridae), cornea loosen microbacterium (Microbacterium keratanolyticum) and celebrating sheng, a reed pipe wind instrument it is red
Coccus (Rhodococcus qingshengii).
4. a kind of preparation method of compound fixation support of PVA-SA for embedding degrading microorganism as described in claim 1,
It is characterized in transferring in LB liquid medium the preparation method comprises the following steps: bacterium will be inoculated with for the bacteria suspension, 30 DEG C, 180rmp environment culture
48h obtains seed liquor, and above-mentioned 1mL seed liquor is inoculated into the LB culture medium of 100mL, 180r/min, 30 DEG C culture for 24 hours,
6000r/min, 4 DEG C of centrifugation 10min, discard supernatant, and are blown and beaten and are resuspended with sterile water, and centrifugation is abandoned supernatant, is so repeated 3 times, will
To thallus be prepared into OD with inorganic salt solution600For 2.0 bacteria suspension, the inorganic salt content of the inorganic salt solution is NaCl
1.0g/L, NH4NO31.0g/L, K2HPO41.5g/L, KH2PO40.5g/L, MgSO4·7H2O 0.2g/L, 1mL microelement
Stock solution/L, the proportion of the microelement stock solution are KI 0.005g, MnSO4·4H2O 0.2g, CuSO4·2H2O
0.02g, ZnSO4·7H2O 0.2g, Na2MoO4·2H2O 0.25g, H3BO30.008g, FeCl3·6H2O 0.1g, adds water to
100mL；By the polyvinyl alcohol of 100g/L, the sodium alginate of 10g/L in the composite material solution, the silica of 30g/L,
The deionized water of the calcium carbonate of 5g/L, the active carbon of 6g/L and surplus is formulated, and the crosslinking agent is by saturation boric acid solution
Dissolution mass fraction be 2% calcium chloride solid and with the sodium hydroxide tune pH of 0.5mol/L be 6.22 after obtain, the hardening
Treatment conditions are the crosslinking Treatment 12~for 24 hours under the conditions of 4 DEG C.
5. a kind of preparation method of compound fixation support of PVA-SA for embedding degrading microorganism as claimed in claim 4,
The volume ratio for being characterized in the bacteria suspension and composite material solution is 18~22:100.
6. using such as a kind of compound fixation support of PVA-SA of embedding degrading microorganism as described in Claims 1 to 5 is any
The fixation support that preparation method obtains.
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| CN201910788354.XA CN110452900B (en) | 2019-08-26 | 2019-08-26 | Preparation method of PVA-SA composite immobilized carrier for embedding degradation microorganisms |
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| CN201910788354.XA CN110452900B (en) | 2019-08-26 | 2019-08-26 | Preparation method of PVA-SA composite immobilized carrier for embedding degradation microorganisms |
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