CN106517537A - Method for improving activity of anaerobic ammonium oxidation embedded particles - Google Patents
Method for improving activity of anaerobic ammonium oxidation embedded particles Download PDFInfo
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- CN106517537A CN106517537A CN201611005912.3A CN201611005912A CN106517537A CN 106517537 A CN106517537 A CN 106517537A CN 201611005912 A CN201611005912 A CN 201611005912A CN 106517537 A CN106517537 A CN 106517537A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
Abstract
The invention discloses a method for improving activity of anaerobic ammonium oxidation embedded particles and belongs to the field of wastewater treatment. Quorum sensing signal molecules of AHLs are added to seed sludge when anaerobic ammonia oxidation seed sludge is cultured and acclimatized, the cultured sludge is mixed with a PVA-SA (polyvinyl acetate-sodium alginate) embedding agent, signal molecules are added again in the mixing process, and the PVA-SA anaerobic ammonium oxidation embedded particles are prepared after the mixture is crosslinked for several hours. With the adoption of the method, the activity is substantially improved, the preparation process is simple, the operation is convenient and the performance is stable.
Description
Technical field
The invention belongs to field of waste water treatment, the immobilized microorganism technology commonly used in being related to wastewater treatment process, specifically
It is related to a kind of method that can improve Anammox embedded particles activity.
Background technology
Denitrification process based on Anammox is high with its nitric efficiency, and energy consumption is given birth in waste water with low cost and other advantages
Have a good application prospect in thing denitrification process.In this course, anaerobic ammonia oxidizing bacteria is with nitrite as electron acceptor,
It is nitrogen by ammonium oxidation.Compared with traditional denitrification process, Anaerobic ammonium oxidation system has oxygen demand few, is not required to additional organic carbon
Source, the few advantage of the surplus sludge volume of generation.And correlative study shows that anaerobic ammonia oxidizing bacteria growth rate is extremely slow, during multiplication
Between be up to 11 days, and anaerobic ammonium oxidation sludge be anammox bacterium, anaerobic bacteria, amphimicrobe and aerobic bacteria composition complicated micro- life
Thing syntaxial system, therefore often lead to be difficult to maintain higher anaerobic ammonia oxidation microbiological amount in reactor.It is anti-in order to realize
The high concentration of anaerobic ammonia oxidation microbiological, low-bleed in device are answered, using immobilized microorganism technology by anaerobic ammonia oxidation microbiological
It is fixed in the area of space of restriction so as to keep activity and can recycle.The performance of various embedded materials is compared
It is preferred that determining the embedding material for using the mixing material of polyvinyl alcohol (PVA) and sodium alginate (SA) as anaerobic ammonia oxidizing bacteria afterwards
Material.There is a bottleneck in anaerobic ammonia oxidizing bacteria immobilization technology to be difficult to make microorganism in FX with higher
Density, and due to there is resistance to mass tranfer, embedding bacterium anaerobic ammoxidation activity is not high.And AHLs class signaling molecule controllable grams
The physiological behavior of negative bacterium, affects the indexs such as flora growth rate and generation length, affects Bacterial community and dominant bacteria, so as to shadow
Ring sludge Tiny ecosystem composition and function.
The content of the invention
Based on problems of the prior art, the present invention provide one kind can improve Anammox embedding bacterium activity and
The method of nitrogen removal performance and application, are difficult to improve density and activity of the anaerobic ammonia oxidizing bacteria in FX in solving prior art
Not high to cause to embed bacterium stability in denitrogenation of waste water processing procedure limited, to the treatment effeciency of the pollutants such as nitrogen in waste water less
Preferable problem.
In order to achieve the above object, the present invention is by following technical solution realizing：
It is a kind of can improve Anammox embedded particles activity method, culture domestication Anammox kind mud when to
AHLs colony induction signaling molecules are added in planting mud, then the sludge of culture is mixed with PVA-SA embedding mediums, in mixed process
Signaling molecule is added again, is prepared into PVA-SA Anammox embedded particles after crosslinking a few hours.Concrete operation step and work
Skill condition is as follows：
(1) plant the culture of mud：Appropriate mud of planting is taken to sbr reactor device, the nutrient solution containing ammonia nitrogen and nitrite nitrogen, nutrient solution is added
The concentration of middle ammonia nitrogen and nitrite nitrogen is 4.5mmol/L, then to sbr reactor device and adds certain density signaling molecule, ring
Border temperature is 32～33 DEG C, and pH is 7.8～8.3, DO concentration in below 0.15mg/L, carries out occasional drive, changes nutrition every time
Signaling molecule, batch (-type) culture 30 days are added during liquid；
(2) preparation of embedding medium：Weigh a certain amount of polyvinyl alcohol (PVA) and sodium alginate (SA) is added to the water, heat
Dissolving, is well mixed；
For improve mechanical strength, also certain density Powdered Activated Carbon can be added in above-mentioned solution, be stirred,
Make embedding medium；
(3) plant mud to mix with embedding medium：With the anaerobism after potassium bicarbonate buffer rinsing step (1) culture that pH is 7.0
Ammoxidation kind mud removes surface impurity the inorganic carbon source of supplement and basicity, is then centrifuged, is finally mixed with embedding medium,
The signaling molecule with same type and concentration in step (1) is added in mixed process；
(4) Anammox embeds the preparation of bead：Step (3) cultured Anammox kind mud is mixed with embedding medium
After closing uniformly, mixed liquor is added dropwise to into the CaCl of 2～6wt%2In solution, 4 DEG C of sealing lucifuge is crosslinked 12 hours, consolidate
The Anammox bead of fixedization, it is standby after then being cleaned with phosphate buffer and aqua sterilisa；
(5) Anammox embeds the activation of bead：Before sewage is processed, Anammox is embedded into bead activation culture
One week.
In described embedding medium, the content of PVA is 6～10wt%, the content of SA is 2～4wt%, and powder activated carbon contains
Measure as 8～12g/L.
Described crosslinking agent is CaCl2Solution, for CaCl2Its mass percent of solution is 2～6wt%.
Described signaling molecule is AHLs, and for AHLs, only C4-HSL can improve the work that Anammox embeds bead
Property.
The concentration of C4-HSL is 0.05 μM～3.0 μM, and preferred concentration can significantly improve Anammox embedding when being 1.5 μM
The activity of bead, when continuing to increase concentration, its activity does not continue to improve.When signaling molecule is added, first will be believed using organic solvent
Number molecular melting, typically using dimethyl sulfoxide (DMSO) (DMSO) or dimethylformamide (dimethyl formamide), solubility
For 20mg/mL～30mg/mL.
Described Anammox embedding bead embedding medium and anaerobic ammonium oxidation sludge volume ratio are 1：0.5～1:2.
Described Anammox embedding bead particle diameter is 3～5mm.
Described Anammox embedding bead activation is referred to uses target waste water batch experiments one under 32 DEG C of temperature conditionss
In week, it is thus capable of sufficiently recovering immobilized anaerobic ammonia oxidation microbiological activity.
Anammox embedded particles obtained in the inventive method are used for sludge denitrification.
The present invention adds the signaling molecule of optium concentration (preferred concentration) in the Anammox kind mud for taking out, using battalion
Nutrient solution culture 30 days, then by the Anammox kind mud through potassium bicarbonate buffer flushing after be centrifuged, with embedding medium according to
Certain volume adds the signaling molecule of same concentrations same type again than uniform mixing in mixed process.Will be mixed with peristaltic pump
Close liquid and be added dropwise to crosslinker solution (CaCl2Solution) in, sealing lucifuge 4 DEG C of crosslinkings, 12 hours prepared immobilization Anammoxs
Bead, refrigerates in being put into 4 DEG C of physiological saline standby after immobilized spherule is cleaned.Before processing to target waste water, detest
Anaerobic ammonium oxidation embedding bead experiences one-week activation stage.Target waste water nitrite concentration too high (can not be less than
200mg/L), in order to avoid producing stronger inhibitory action to anaerobic ammonia oxidation microbiological.
The action principle of the present invention：Immobilization technology is to hold the effective means for staying biomass, but still there is microorganism and exist
FX density is not high, and due to resistance to mass tranfer, embeds the not high problem of bacterium anaerobic ammoxidation activity.By signaling molecule C4-
HSL reuses embedded material PVA-SA by anaerobic ammonium oxidation sludge immobilization in being added to Anammox kind mud, significantly improve
The anaerobic ammoxidation activity of immobilization Anammox bead.This is because in culture Anammox kind mud initial stage and embedding
During add signaling molecule C4-HSL, when the concentration of C4-HSL reach threshold value (present invention in C4-HSL threshold value be 1.5 μ
Can be arrived by microorganism detection when M), now signaling molecule can reach purpose position into cell interior by the transhipment of cell membrane effect
The expression of related gene is put and then regulates and controls, control is catalyzed the synthesis of the enzyme of Anammox reaction, such as nitrite reductase etc.,
Cause a signal transduction to start, and finally make body behavior change, promote the ratio of anaerobic ammonia oxidizing bacteria to detest
Anaerobic ammonium oxidation activity is greatly improved, and anaerobic ammonia oxidizing bacteria value-added speed is also accelerated.
Compared to the prior art the present invention, has the advantage that and effect：
(1) anaerobic ammoxidation activity is high.Signaling molecule is added in anaerobic ammonium oxidation sludge, using signaling molecule C4-HSL
The anaerobic ammoxidation activity of anaerobic ammonia oxidation microbiological is made substantially to carry the regulating and controlling effect of anaerobic ammonia oxidation microbiological physiological behavior
It is high.
(2) in embedded immobilization bead, anaerobic ammonia oxidation microbiological quantity is improved.Signaling molecule C4-HSL improves anaerobism
The growth rate of ammonia oxidation bacteria, increases microbial biomass in embedding bead.
(3) enhance the inter-species cooperation of Gram-negative bacteria in Anammox embedding bead.Anaerobic ammonium oxidation sludge is
Many bacterium mixed things, wherein anaerobic ammonia oxidizing bacteria are dominant bacteria, also have nitrifier, denitrifying bacterium etc. concurrently, and signaling molecule C4-HSL is not
It is only capable of the physiological behavior of anaerobic ammonia oxidizing bacteria, also can be used as anaerobic ammonia oxidizing bacteria, nitrobacteria and denitrifying bacteria etc.
AC signal, promotes which to cooperate with each other, mutualism.
(4) preparation process is simple, easy to operate, stable performance.
Description of the drawings
Fig. 1 is ammonia nitrogen when embodiment 1 adds unlike signal molecule, nitrite nitrogen and nitrate nitrogen concentration changes with time curve；Fig. 1
A () is that ammonia nitrogen concentration changes over curve；Fig. 1 (b) is nitrite nitrogen concentration changes with time curve；Fig. 1 (c) is nitrate nitrogen concentration
Change over curve.
Fig. 2 be 2 embedding medium of embodiment from sludge volume than it is different when to add ammonia nitrogen after signaling molecule, nitrite nitrogen and nitrate nitrogen dense
Degree changes over curve；Fig. 2 (a) is that ammonia nitrogen concentration changes over curve；Fig. 2 (b) is nitrite nitrogen concentration changes with time
Curve；Fig. 2 (c) is nitrate nitrogen concentration changes with time curve.
Specific embodiment
Present disclosure is described in further details below in conjunction with drawings and Examples, but embodiments of the present invention
Not limited to this.In terms of using PVA-SA mixing embedded material embedding anaerobic ammonium oxidation sludge, the letter of suitable type can be found
Number molecule, and the signaling molecule can improve PVA-SA Anammox embedded particles Activity and stabill it is unclear,
Correlative study also belongs to blank.Therefore this research is chosen three kinds of AHLs signaling molecules (C4-HSL, C6-HSL and C8-HSL) and is artificially added
It is added in anaerobic ammonium oxidation sludge, investigates the impact that signaling molecule embeds bacterium activity to Anammox, to can be further
Improve effect of the Anammox technology in denitrogenation of waste water.
Material：
Anaerobic ammonium oxidation sludge：Take from the stable operation UASB reactors top floc sludge of 2 years, the sludge Jing of taking-up
PBS (0.1M, pH7.4) is rinsed 3 times, removes the residual matrix of Sludge Surface, 4000r m-1It is standby after centrifugation 10min.
Embedded material：Embedded material used be polyvinyl alcohol (PVA, the degree of polymerization 1750 ± 50, saponification degree 98.5%) and
Sodium alginate (SA, viscosity 150mpa s), is analysis pure.
Signaling molecule：N- [(3S)-tetrahydro-2-oxo-3-furanyl]-butanamide (Chineses：N- fourths
Acyl group-L- homoserine lactones, abbreviation C4-HSL)；N-[(3S)-tetrahydro-2-oxo-3-furanyl]-
Hexanamide (Chineses：N- caproyl-L- homoserine lactones, abbreviation C6-HSL)；N-[(3S)-tetrahydro-2-
Oxo-3-furanyl] (Chinese is-octanamide：N- caprylyl-L- homoserine lactones, referred to as：C8-HSL),
Purchased from sigma-aldrich companies of the U.S..
It should be noted that the AHLs in AHLs colony induction signaling molecules refers to N- acyl homoserine lactones monoids
Body induction signal molecule.
Defer to above-mentioned technical proposal, the specific embodiment of the present invention given below, it should be noted that the present invention not office
It is limited to specific examples below, all equivalents done on the basis of technical scheme each fall within the protection model of the present invention
Enclose.The present invention is described in further details with reference to embodiment.
Embodiment 1：Unlike signal molecule embeds the impact of bead activity to Anammox
Be separately added in the Anammox kind mud for taking out nutrient solution and different signaling molecule C4-HSL, C6-HSL,
C8-HSL, the concentration that adds of three kinds of signaling molecules are respectively set to 0.05 μM, 1.5 μM and 3.0 μM (three kinds of signaling molecules adopt two
Methyl sulfoxide is first dissolved, and three kinds of signaling molecule solution concentrations of preparation are 20mg/mL), using nutrient solution and signal point
Sub- solution batch culture 30 days (being a cycle when water outlet ammonia nitrogen concentration is the 20% of influent ammonium concentration), then by the anaerobism
Ammoxidation kind mud is centrifuged after the potassium bicarbonate buffer that pH is 7.0 or so rinses 3 times.Mass fraction is prepared for 10wt%'s
Polyvinyl alcohol (PVA) and 2wt% sodium alginates (SA) mixed liquor, heating for dissolving are well mixed.For improve mechanical strength, then
8g/L Powdered Activated Carbons are added, is stirred, is made mixing embedding medium.In nutrient solution, the concentration of ammonia nitrogen and nitrite nitrogen is
4.5mmol/L。
By the Anammox kind mud after centrifugation and embedding medium by volume 1:1 is well mixed, and adds in mixed process again
Enter the signaling molecule of same type and concentration.Then above-mentioned mixed liquor is added dropwise to into crosslinking agent CaCl using peristaltic pump2It is molten
In liquid, being fixed Anammox bead (3～5mm of particle diameter) after being crosslinked 12 hours in 4 DEG C of refrigerators after sealing shading, is placed in,
Cleaned with aqua sterilisa the Anammox bead for preparing refrigerate in 4 DEG C of refrigerators it is standby.Appropriate immobilized spherule is taken, is added
Target waste water, cultivates one week (concussion three times, every time one hour daily), in 32 DEG C of constant-temperature shaking incubators when ammonia nitrogen in water outlet
Concentration be reduced to into water 20% when for an activity cycle, change to fresh target waste water after end cycle, activation one week is with extensive
The activity of multiple Anammox bead.
The Anammox bead 50mL after activity recovery is taken, is proceeded in the band plug serum bottle of 500mL, serum bottle black
Reflective sheeting is wrapped up.Add 400mL target waste water, be placed in constant temperature blender with magnetic force, by the logical high pure nitrogen 30min of air inlet with
Oxygen in stripping reactor ensures anaerobic environment (dissolved oxygen concentration is less than 0.15mg/L).Magnetic stirring apparatus rotating speed 150r/
Min, temperature are kept for 32 DEG C, do not control pH.Take the anaerobism ammonia oxygen for not adding signaling molecule that activation is prepared under 50mL the same terms
Change bead and synchronize parallel test as a control group.Ammonia nitrogen in waste water concentration and nitrite concentration be respectively 95mg/L and
125mg/L, reaction time are 48 hours, are sampled by air inlet with syringe every 8 hours, and in determining water sample, " three nitrogen " is dense
Degree.
Embodiment 2：Embedding medium is added after 1.5 μM of C4-HSL with Anammox kind mud volume ratio to Anammox bag
Bury the impact of bead activity
Carried out embedding medium 1 has been respectively with Anammox kind mud volume ratio：0.5,1:1,1:Signaling molecule when 2 is to detesting
Anaerobic ammonium oxidation embeds the experiment of bead activity influence, and laboratory operating procedures are with embodiment 1.
Test and analysis method：
Ammonia nitrogen determination adopts Nessler's reagent photometer, nitrate nitrogen to determine and adopt ultraviolet spectrophotometry, and nitrous nitrogen determination is adopted
N- (1- naphthyls)-ethylenediamine photometry (using ultraviolet specrophotometer DR5000, Hash company of the U.S.), DO are determined and are adopted
HQ30d dissolved oxygen meters (Hash company of the U.S.), pH are determined using pHS-3c pH meters (Shanghai Precision Scientific Apparatus Co., Ltd).
As a result with analysis：
During 0～16 hour, the concentration for adding ammonia nitrogen and nitrite in 1.5 μM of C4-HSL groups is minimum, and by folding
Line slope can be seen that the group is most fast (accompanying drawing 1) to the degradation rate of ammonia nitrogen and nitrite, after illustrating to add 1.5 μM of C4-HSL
Anammox embedding bead anaerobic ammoxidation activity is largely improved.During 16～48 hours, 1.5 μM of C4- are added
HSL groups the degradation speed of ammonia nitrogen and nitrite nitrogen is gradually slowed down it is last in addition it is close do not add signaling molecule group, this is because base
Matter concentration is greatly reduced, and serves restriction effect.Other two kinds of signaling molecules C6-HSL and C8-HSL are added then to anaerobism ammonia oxygen
Change embedding bead activity without impact.Adding 1.5 μM of C4-HSL groups final monitoring result after 48 hrs is：Ammonia nitrogen concentration
For 0.97mg/L, clearance reaches 98.9%, and nitrous nitrogen concentration is 1.55mg/L, clearance up to 99%, the removal amount of nitrite nitrogen
Ratio with ammonia nitrogen removal amount is 1.31, is close to theoretical value 1.32.Illustrate the Anammox after adding 1.5 μM of C4-HSL
Embedding bead shows higher anaerobic ammoxidation activity, has a good application prospect.Fig. 2 shows to add signal point simultaneously
After son, embedding medium compares Anammox embedding bead activity with sludge volume and also has a major impact.Embedding sludge content is less
(volume ratio 1:0.5), when, Anammox embedding bead activity is not high, because micro organism quantity is not enough；Higher (the body of sludge content
Product compares 1:2) when, Anammox embedding bead activity is not equally high, because now Anammox embedding bead mechanical strength
Low, easily swelling, broken, unstable, optimum volume ratio is 1:1.
Claims (9)
1. it is a kind of can to improve the active method of Anammox embedded particles, it is characterised in that to comprise the following steps：(1) plant
The culture of mud：Appropriate mud of planting is taken to sbr reactor device, the nutrient solution containing ammonia nitrogen and nitrite nitrogen, ammonia nitrogen and nitrous in nutrient solution is added
The concentration of nitrogen is 4.5mmol/L, then to sbr reactor device and adds certain density signaling molecule, and environment temperature is 32～
33 DEG C, pH is 7.8～8.3, DO concentration in below 0.15mg/L, carries out occasional drive, adds signal when changing nutrient solution every time
Molecule, batch (-type) culture 30 days；
(2) preparation of embedding medium：Weigh a certain amount of polyvinyl alcohol (PVA) and sodium alginate (SA) be added to the water, heating for dissolving,
It is well mixed；
Or for improve mechanical strength, add certain density Powdered Activated Carbon in stating embedding agent solution further up, stir
Mix uniform, make mixing embedding medium；
(3) plant mud to mix with embedding medium：With the anaerobism ammonia oxygen after potassium bicarbonate buffer rinsing step (1) culture that pH is 7.0
Change kind of mud and remove surface impurity the inorganic carbon source of supplement and basicity, be then centrifuged, finally mixed with embedding medium, mixed
During add with step (1) in same type and concentration signaling molecule；
(4) Anammox embeds the preparation of bead：Step (3) cultured Anammox kind mud is mixed with embedding medium
After even, mixed liquor is added dropwise to into the CaCl of 2～6wt%2In solution, 4 DEG C of sealing lucifuge is crosslinked 12 hours, being fixed
Anammox bead, it is standby after then being cleaned with phosphate buffer and aqua sterilisa；
(5) Anammox embeds the activation of bead：Before sewage is processed, by Anammox embedding bead activation culture one
Week.
2. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
In embedding medium, the content of PVA is 6～10wt%, and the content of SA is 2～4wt%, and the content of powder activated carbon is 8～12g/L.
3. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
Signaling molecule is C4-HSL.
4. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
The concentration of C4-HSL is 0.05 μM～3.0 μM.
5. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
The concentration of C4-HSL is 1.5 μM.
6. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
It is 1 that Anammox embeds bead embedding medium with anaerobic ammonium oxidation sludge volume ratio：0.5～1:2.
7. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
Anammox embedding bead particle diameter is 3～5mm.
8. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
Anammox embedding bead activation referred under 32 DEG C of temperature conditionss with target waste water batch experiments one week, makes immobilized to detest
Anaerobic ammonium oxidation microbial activity is thus capable of sufficiently recovering.
9. according to a kind of method that can improve Anammox embedded particles activity described in claim 1, it is characterised in that
When signaling molecule is added, dissolved using organic solvent.
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| CN113461162A (en) * | 2021-07-16 | 2021-10-01 | 北京工业大学 | Method and device for improving urban domestic sewage treatment performance of ANAMMOX process |
| CN115011585A (en) * | 2022-06-21 | 2022-09-06 | 重庆大学 | Anaerobic ammonium oxidation bacteria-shortcut nitrifying bacteria co-embedded pellet and preparation method and application thereof |
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| CN105923760A (en) * | 2016-06-16 | 2016-09-07 | 北京工业大学 | Apparatus and operation method for quickly forming anammox particles using quorum sensing mechanism |
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