CN106517537B - A kind of active method of raising Anammox embedded particles - Google Patents
A kind of active method of raising Anammox embedded particles Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
Abstract
A kind of active method of raising Anammox embedded particles, belongs to field of waste water treatment.AHLs colony induction signaling molecule is added into kind of mud when Anammox kind mud is tamed in culture, then the sludge of culture is mixed with PVA-SA embedding medium, signaling molecule is added in mixed process again, is prepared into PVA-SA Anammox embedded particles after being crosslinked a few hours.Using the method for the present invention, activity is increased substantially, and preparation process is simple, easy to operate, and performance is stablized.
Description
Technical field
The invention belongs to field of waste water treatment, it is related to common immobilized microorganism technology in wastewater treatment process, specifically
Being related to one kind can be improved the active method of Anammox embedded particles.
Background technique
Denitrification process based on Anammox with its nitric efficiency height, energy consumption and it is at low cost the advantages that given birth in waste water
It has a good application prospect in object denitrification process.In this course, anaerobic ammonia oxidizing bacteria is using nitrite as electron acceptor,
It is nitrogen by ammonium oxidation.Compared with traditional denitrification process, Anaerobic ammonium oxidation system has oxygen demand few, is not required to additional organic carbon
Source, the few advantage of the surplus sludge volume of generation.And correlative study shows that anaerobic ammonia oxidizing bacteria growth rate is extremely slow, when multiplication
Between be up to 11 days, and anaerobic ammonium oxidation sludge is the micro- life of complexity of anammox bacterium, anaerobic bacteria, amphimicrobe and aerobic bacteria composition
Object syntaxial system, therefore often lead to be difficult to maintain higher anaerobic ammonia oxidation microbiological amount in reactor.It is anti-in order to realize
The high concentration of anaerobic ammonia oxidation microbiological, low-bleed in device are answered, using immobilized microorganism technology by anaerobic ammonia oxidation microbiological
It is fixed in the area of space of restriction, it is made to keep activity and can recycle.The performance of various embedded materials is compared
It is preferred that determination uses embedding material of the mixing material of polyvinyl alcohol (PVA) and sodium alginate (SA) as anaerobic ammonia oxidizing bacteria afterwards
Material.It there are a bottleneck is exactly to be difficult to keep microorganism with higher in fixed area in anaerobic ammonia oxidizing bacteria immobilization technology
Density, and since there are resistance to mass tranfers, it is not high to embed bacterium anaerobic ammoxidation activity.And the controllable gram of AHLs class signaling molecule
The physiological behavior of negative bacterium influences the indexs such as flora growth rate and generation length, influences Bacterial community and dominant bacteria, thus shadow
Ring sludge Tiny ecosystem composition and function.
Summary of the invention
Based on problems of the prior art, the present invention provide one kind can be improved Anammox embedding bacterium activity and
The method and application of nitrogen removal performance, solution are difficult to improve anaerobic ammonia oxidizing bacteria in the density and activity of fixed area in the prior art
Not high to lead to embed bacterium stability in denitrogenation of waste water treatment process limited, less to the treatment effeciencies of the pollutants such as nitrogen in waste water
Ideal problem.
In order to achieve the above object, the present invention realizes by following technical solution:
One kind can be improved the active method of Anammox embedded particles, culture tame Anammox kind mud when to
AHLs colony induction signaling molecule is added in kind mud, then mixes the sludge of culture with PVA-SA embedding medium, in mixed process
Signaling molecule is added again, is prepared into PVA-SA Anammox embedded particles after being crosslinked a few hours.Concrete operation step and work
Skill condition is as follows:
(1) it plants the culture of mud: taking appropriate kind of mud to sbr reactor device, the nutrient solution containing ammonia nitrogen and nitrite nitrogen, nutrient solution is added
The concentration of middle ammonia nitrogen and nitrite nitrogen is 4.5mmol/L, then to sbr reactor device and certain density signaling molecule, ring is added
Border temperature is 32~33 DEG C, and pH is 7.8~8.3, DO concentration in 0.15mg/L hereinafter, progress occasional drive, replaces nutrition every time
Signaling molecule, intermittent culture 30 days are added when liquid；
(2) preparation of embedding medium: weighing a certain amount of polyvinyl alcohol (PVA) and sodium alginate (SA) is added to the water, heating
Dissolution is uniformly mixed；
In order to improve mechanical strength, certain density Powdered Activated Carbon can be also added into above-mentioned solution, is stirred evenly,
Embedding medium is made；
(3) kind mud is mixed with embedding medium: the anaerobism after being cultivated with the potassium bicarbonate buffer rinsing step (1) that pH is 7.0
Ammoxidation kind mud removal surface impurity simultaneously supplements inorganic carbon source and basicity, is then centrifuged, is finally mixed with embedding medium,
It is added in mixed process and the signaling molecule of same type and concentration in step (1)；
(4) preparation of Anammox embedding bead: the cultured Anammox kind mud of step (3) and embedding medium is mixed
After closing uniformly, mixed liquor is added dropwise to the CaCl of 2~6wt%2In solution, sealing is protected from light 4 DEG C and is crosslinked 12 hours, consolidate
Surely the Anammox bead changed, it is spare after then being cleaned with phosphate buffer and aqua sterilisa；
(5) before handling sewage, Anammox the activation of Anammox embedding bead: is embedded into bead activation culture
One week.
The content of PVA is 6~10wt% in the embedding medium, and the content of SA is 2~4wt%, and powder activated carbon contains
Amount is 8~12g/L.
The crosslinking agent is CaCl2Solution, for CaCl2Its mass percent of solution is 2~6wt%.
The signaling molecule is AHLs, and the work of Anammox embedding bead can be improved for AHLs, only C4-HSL
Property.
The concentration of C4-HSL is 0.05 μM~3.0 μM, and preferred concentration can significantly improve Anammox embedding when being 1.5 μM
The activity of bead, its activity does not continue to improve when continuing growing concentration.When signaling molecule is added, it will first be believed using organic solvent
Number molecular melting generally uses dimethyl sulfoxide (DMSO) or dimethylformamide (dimethyl formamide), solubility
For 20mg/mL~30mg/mL.
The Anammox embedding bead embedding medium and anaerobic ammonium oxidation sludge volume ratio are 1:0.5~1:2.
The Anammox embedding bead partial size is 3~5mm.
The Anammox embedding bead activation refers under the conditions of 32 DEG C of temperature with target waste water batch experiments one
Week it is thus capable of sufficiently recovering the anaerobic ammonia oxidation microbiological activity of immobilization.
Anammox embedded particles made from the method for the present invention are used for sludge denitrification.
The signaling molecule of optium concentration (preferred concentration) is added in the present invention in the Anammox kind mud of taking-up, uses battalion
Nutrient solution culture 30 days, then the Anammox kind mud is centrifuged after potassium bicarbonate buffer rinses, with embedding medium according to
Certain volume is mixed than uniformly, and the signaling molecule of same concentrations same type is added in mixed process again.It will be mixed with peristaltic pump
It closes liquid and is added dropwise to crosslinker solution (CaCl2Solution) in, sealing is protected from light 4 DEG C of crosslinkings, 12 hours obtained immobilization Anammoxs
Bead, be put into after immobilized spherule is cleaned in 4 DEG C of physiological saline refrigerate it is spare.Before handling target waste water, detest
Anaerobic ammonium oxidation embeds bead and undergoes one-week activation stage.Nitrite concentration excessively high (can not be lower than in target waste water
200mg/L), in order to avoid generating stronger inhibiting effect to anaerobic ammonia oxidation microbiological.
Action principle of the invention: immobilization technology is the effective means for withholding biomass, but still remains microorganism and exist
Fixed area density is not high, and due to resistance to mass tranfer, embeds the not high problem of bacterium anaerobic ammoxidation activity.By signaling molecule C4-
HSL is added in Anammox kind mud and reuses embedded material PVA-SA for anaerobic ammonium oxidation sludge immobilization, significantly improves
The anaerobic ammoxidation activity of immobilization Anammox bead.This is because in culture Anammox kind mud initial stage and embedding
Signaling molecule C4-HSL is added in the process, (threshold value of C4-HSL is 1.5 μ in the present invention when the concentration of C4-HSL reaches threshold value
It can be arrived by microorganism detection when M), signaling molecule can be acted on by the transhipment of cell membrane at this time enters cell interior arrival purpose position
The expression of related gene, the synthesis of the enzyme of control catalysis Anammox reaction, such as nitrite reductase are set and then regulate and control,
Cause a signal transduction to start, and body behavior is finally made to change, the ratio of anaerobic ammonia oxidizing bacteria is promoted to detest
Anaerobic ammonium oxidation activity greatly improves, and anaerobic ammonia oxidizing bacteria value-added speed is also accelerated.
Compared to the prior art the present invention, has the following advantages that and effect:
(1) anaerobic ammoxidation activity is high.Signaling molecule is added in anaerobic ammonium oxidation sludge, utilizes signaling molecule C4-HSL
Mention the anaerobic ammoxidation activity of anaerobic ammonia oxidation microbiological obviously the regulating and controlling effect of anaerobic ammonia oxidation microbiological physiological behavior
It is high.
(2) anaerobic ammonia oxidation microbiological quantity improves in embedded immobilization bead.Signaling molecule C4-HSL improves anaerobism
The growth rate of ammonia oxidation bacteria makes to embed microbial biomass in bead and increases.
(3) the inter-species cooperation of Gram-negative bacteria in Anammox embedding bead is enhanced.Anaerobic ammonium oxidation sludge is
More bacterium mixed things, wherein anaerobic ammonia oxidizing bacteria is dominant bacteria, also has nitrifier, denitrifying bacterium etc. concurrently, signaling molecule C4-HSL is not
It is only capable of the physiological behavior of regulation anaerobic ammonia oxidizing bacteria, also can be used as anaerobic ammonia oxidizing bacteria, nitrobacteria and denitrifying bacteria etc.
AC signal promotes it to cooperate with each other, mutualism.
(4) preparation process is simple, easy to operate, and performance is stablized.
Detailed description of the invention
Fig. 1 is ammonia nitrogen when embodiment 1 adds unlike signal molecule, nitrite nitrogen and nitrate nitrogen concentration changes with time curve；Fig. 1
It (a) is that ammonia nitrogen concentration changes over time curve；Fig. 1 (b) is nitrite nitrogen concentration changes with time curve；Fig. 1 (c) is nitrate nitrogen concentration
Change over time curve.
It is denseer than not adding ammonia nitrogen after signaling molecule, nitrite nitrogen and nitrate nitrogen simultaneously with sludge volume that Fig. 2 is 2 embedding medium of embodiment
Degree changes over time curve；Fig. 2 (a) is that ammonia nitrogen concentration changes over time curve；Fig. 2 (b) is nitrite nitrogen concentration changes with time
Curve；Fig. 2 (c) is nitrate nitrogen concentration changes with time curve.
Specific embodiment
The contents of the present invention are described in further details below in conjunction with drawings and examples, but embodiments of the present invention
It is without being limited thereto.In terms of using PVA-SA mixing embedded material embedding anaerobic ammonium oxidation sludge, the letter of suitable type can be found
Number molecule, and the signaling molecule can improve PVA-SA Anammox embedded particles Activity and stabill it is unclear,
Correlative study also belongs to blank.Therefore this research is chosen three kinds of AHLs signaling molecules (C4-HSL, C6-HSL and C8-HSL) and is artificially added
It is added in anaerobic ammonium oxidation sludge, investigates signaling molecule and the active influence of bacterium is embedded on Anammox, to can be further
Improve effect of the Anammox technology in denitrogenation of waste water.
Material:
Anaerobic ammonium oxidation sludge: it is derived from stable operation 2 years UASB reactor top floc sludges, the sludge warp of taking-up
PBS (0.1M, pH7.4) is rinsed 3 times, removes the residual matrix of Sludge Surface, 4000rm-1It is spare after centrifugation 10min.
Embedded material: embedded material used be polyvinyl alcohol (PVA, the degree of polymerization 1750 ± 50, saponification degree 98.5%) and
Sodium alginate (SA, viscosity 150mpas) is that analysis is pure.
Signaling molecule: N- [(3S)-tetrahydro-2-oxo-3-furanyl]-butanamide (Chinese: N- fourth
Acyl group-L- homoserine lactone, abbreviation C4-HSL)；N-[(3S)-tetrahydro-2-oxo-3-furanyl]-
Hexanamide (Chinese: N- caproyl-L- homoserine lactone, abbreviation C6-HSL)；N-[(3S)-tetrahydro-2-
Oxo-3-furanyl]-octanamide (Chinese are as follows: N- caprylyl-L- homoserine lactone, referred to as: C8-HSL),
Purchased from sigma-aldrich company, the U.S..
It should be noted that the AHLs in AHLs colony induction signaling molecule refers to N- acyl homoserine lactones monoid
Body induction signal molecule.
In compliance with the above technical solution, specific embodiments of the present invention are given below, it should be noted that the present invention not office
It is limited to following specific embodiments, all equivalent transformations made on the basis of the technical solutions of the present application each falls within protection model of the invention
It encloses.The present invention is described in further details below with reference to embodiment.
Embodiment 1: unlike signal molecule embeds the active influence of bead to Anammox
Be separately added into the Anammox kind mud of taking-up nutrient solution and different signaling molecule C4-HSL, C6-HSL,
The concentration that adds of C8-HSL, three kinds of signaling molecules are respectively set to 0.05 μM, 1.5 μM and 3.0 μM (three kinds of signaling molecules are using two
Methyl sulfoxide is first dissolved, and three kinds of signaling molecule solution concentrations of preparation are 20mg/mL), use nutrient solution and signal point
Sub- solution batch culture 30 days (water outlet ammonia nitrogen concentration is a cycle when being the 20% of influent ammonium concentration), then by the anaerobism
Ammoxidation kind mud is centrifuged after the pH potassium bicarbonate buffer for being 7.0 or so rinses 3 times.Preparing mass fraction is 10wt%'s
Polyvinyl alcohol (PVA) and 2wt% sodium alginate (SA) mixed liquor dissolve by heating, and are uniformly mixed.In order to improve mechanical strength, then
8g/L Powdered Activated Carbon is added, stirs evenly, mixing embedding medium is made.The concentration of ammonia nitrogen and nitrite nitrogen is in nutrient solution
4.5mmol/L。
By the Anammox kind mud after centrifugation, 1:1 is uniformly mixed by volume with embedding medium, is added again in mixed process
Enter the signaling molecule of same type and concentration.Then above-mentioned mixed liquor is added dropwise to crosslinking agent CaCl using peristaltic pump2It is molten
In liquid, sealing shading be placed on be crosslinked 12 hours in 4 DEG C of refrigerators after obtain immobilization Anammox bead (3~5mm of partial size),
Cleaned with aqua sterilisa after the Anammox bead prepared refrigerated in 4 DEG C of refrigerators it is spare.Appropriate immobilized spherule is taken, is added
Target waste water is cultivated one week (shaking daily three times, one hour every time) in 32 DEG C of constant-temperature shaking incubators, when ammonia nitrogen in water outlet
It is an activity cycle when concentration is reduced into the 20% of water, fresh target waste water is changed to after end cycle, activates one week with extensive
The activity of multiple Anammox bead.
Anammox bead 50mL after taking activity recovery is transferred in the band plug serum bottle of 500mL, serum bottle black
Reflective sheeting package.Be added 400mL target waste water, be placed in constant temperature blender with magnetic force, by air inlet lead to high pure nitrogen 30min with
Oxygen in stripping reactor guarantees anaerobic environment (dissolved oxygen concentration is lower than 0.15mg/L).Magnetic stirring apparatus revolving speed 150r/
Min, temperature are kept for 32 DEG C, do not control pH.Take the anaerobism ammonia oxygen for not adding signaling molecule of preparation activation under 50mL the same terms
Change bead and synchronizes parallel test as a control group.Ammonia nitrogen in waste water concentration and nitrite concentration be respectively 95mg/L and
125mg/L, reaction time are 48 hours, are sampled with syringe by air inlet every 8 hours, are measured dense in water sample " three nitrogen "
Degree.
Embodiment 2: embedding medium and Anammox kind mud volume ratio are added after 1.5 μM of C4-HSL to Anammox packet
Bury the active influence of bead
It has carried out embedding medium and Anammox kind mud volume ratio is respectively 1:0.5, signaling molecule when 1:1,1:2 is to detesting
Anaerobic ammonium oxidation embeds the experiment of bead activity influence, and laboratory operating procedures are the same as embodiment 1.
Test and analysis method:
Ammonia nitrogen determination uses Nessler's reagent photometer, and nitrate nitrogen measurement uses ultraviolet spectrophotometry, and nitrous nitrogen determination uses
N- (1- naphthalene)-ethylenediamine photometry (is all made of ultraviolet specrophotometer DR5000, Hash company, the U.S.), and DO measurement uses
HQ30d dissolved oxygen meter (Hash company, the U.S.), pH measurement use pHS-3c pH meter (Shanghai Precision Scientific Apparatus Co., Ltd).
As a result with analysis:
During 0~16 hour, it is minimum to add the concentration of ammonia nitrogen and nitrite in 1.5 μM of C4-HSL groups, and pass through folding
Line slope can be seen that the group is most fast (attached drawing 1) to the degradation rate of ammonia nitrogen and nitrite, illustrates after adding 1.5 μM of C4-HSL
Anammox embedding bead anaerobic ammoxidation activity is largely improved.During 16~48 hours, 1.5 μM of C4- are added
HSL group gradually slow down to the degradation speed of ammonia nitrogen and nitrite nitrogen it is last or even close to not adding signaling molecule group, this is because base
Matter concentration is greatly reduced, and plays restriction effect.Other two kinds of signaling molecules C6-HSL and C8-HSL are added then to anaerobism ammonia oxygen
Changing embedding bead activity does not influence.Add the final monitoring result of 1.5 μM of C4-HSL groups after 48 hrs are as follows: ammonia nitrogen concentration
For 0.97mg/L, removal rate reaches 98.9%, and nitrous nitrogen concentration is 1.55mg/L, and removal rate is up to 99%, the removal amount of nitrite nitrogen
Ratio with ammonia nitrogen removal amount is 1.31, close with theoretical value 1.32.Illustrate to add the Anammox after 1.5 μM of C4-HSL
Embedding bead shows higher anaerobic ammoxidation activity, has a good application prospect.Fig. 2 shows to add signal point simultaneously
Embedding medium compares Anammox embedding bead activity with sludge volume and also has a major impact after son.It is less to embed sludge content
When (volume ratio 1:0.5), Anammox embedding bead activity is not high, because micro organism quantity is insufficient；Higher (the body of sludge content
Product is than 1:2) when, it is equally not high that Anammox embeds bead activity, because Anammox embeds bead mechanical strength at this time
It is low, it is easy swelling, broken, unstable, optimum volume ratio 1:1.
Claims (8)
1. one kind can be improved the active method of Anammox embedded particles, which comprises the following steps:
(1) it plants the culture of mud: taking appropriate kind of mud to sbr reactor device, the nutrient solution containing ammonia nitrogen and nitrite nitrogen, ammonia in nutrient solution is added
The concentration of nitrogen and nitrite nitrogen is 4.5mmol/L, then to sbr reactor device and certain density signaling molecule is added, environment temperature
Degree is 32~33 DEG C, and pH is 7.8~8.3, DO concentration in 0.15mg/L hereinafter, occasional drive is carried out, when replacing nutrient solution every time
Signaling molecule, intermittent culture 30 days is added；
(2) preparation of embedding medium: weighing a certain amount of polyvinyl alcohol (PVA) and sodium alginate (SA) is added to the water, and dissolves by heating,
It is uniformly mixed；
Or in order to improve mechanical strength, is stated in embedding agent solution further up and add certain density Powdered Activated Carbon, stirred
It mixes uniformly, mixing embedding medium is made；
(3) kind mud is mixed with embedding medium: the anaerobism ammonia oxygen after being cultivated with the potassium bicarbonate buffer rinsing step (1) that pH is 7.0
Change kind of mud removal surface impurity and supplement inorganic carbon source and basicity, is then centrifuged, is finally mixed with embedding medium, mixed
It is added in the process and the signaling molecule of same type and concentration in step (1)；
(4) preparation of Anammox embedding bead: the cultured Anammox kind mud of step (3) is mixed with embedding medium
After even, mixed liquor is added dropwise to the CaCl of 2~6wt%2In solution, sealing is protected from light 4 DEG C and is crosslinked 12 hours, obtains immobilization
Anammox bead, it is spare after then being cleaned with phosphate buffer and aqua sterilisa；
(5) before handling sewage, Anammox the activation of Anammox embedding bead: is embedded into bead activation culture one
Week；
Signaling molecule is C4-HSL.
2. one kind described in accordance with the claim 1 can be improved the active method of Anammox embedded particles, which is characterized in that
The content of PVA is 6~10wt% in embedding medium, and the content of SA is 2~4wt%, and the content of powder activated carbon is 8~12g/L.
3. one kind described in accordance with the claim 1 can be improved the active method of Anammox embedded particles, which is characterized in that
The concentration of C4-HSL is 0.05 μM~3.0 μM.
4. one kind described in accordance with the claim 1 can be improved the active method of Anammox embedded particles, which is characterized in that
The concentration of C4-HSL is 1.5 μM.
5. one kind described in accordance with the claim 1 can be improved the active method of Anammox embedded particles, which is characterized in that
Anammox embeds bead embedding medium and anaerobic ammonium oxidation sludge volume ratio is 1:0.5~1:2.
6. one kind described in accordance with the claim 1 can be improved the active method of Anammox embedded particles, which is characterized in that
It is 3~5mm that Anammox, which embeds bead partial size,.
7. one kind described in accordance with the claim 1 can be improved the active method of Anammox embedded particles, which is characterized in that
Anammox embedding bead activation refers under the conditions of 32 DEG C of temperature with target waste water batch experiments one week, makes detesting for immobilization
Anaerobic ammonium oxidation microbial activity is thus capable of sufficiently recovering.
8. one kind described in accordance with the claim 1 can be improved the active method of Anammox embedded particles, which is characterized in that
When signaling molecule is added, dissolved using organic solvent.
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| CN106976973B (en) * | 2017-05-02 | 2020-08-14 | 福瑞莱环保科技（深圳）股份有限公司 | Sewage denitrification method, sewage denitrification agent and preparation method thereof |
| CN109279698B (en) * | 2017-07-21 | 2021-08-27 | 长沙理工大学 | Method for treating sewage with low carbon-nitrogen ratio by using solid carbon source pellets of immobilized denitrifying bacteria |
| CN108483643B (en) * | 2018-04-26 | 2020-09-25 | 北京协同创新研究院 | Anaerobic ammonia oxidation reactor and starting method and operation method thereof |
| CN109231745B (en) * | 2018-09-17 | 2020-06-26 | 知和环保科技有限公司 | Method for accelerating sludge granulation |
| CN110451638A (en) * | 2019-07-29 | 2019-11-15 | 中南大学 | A method of based on colony induction signaling molecule strengthened anaerobic ammoxidation sludge heavy-metal resistance |
| CN110482697B (en) * | 2019-08-01 | 2022-02-18 | 广西大学 | Method for promoting anaerobic digestion and delaying calcification by regulating and controlling anaerobic granular sludge microenvironment by using signal molecules |
| CN110452900B (en) * | 2019-08-26 | 2021-06-01 | 天津科技大学 | Preparation method of PVA-SA composite immobilized carrier for embedding degradation microorganisms |
| CN113461162B (en) * | 2021-07-16 | 2024-04-12 | 北京工业大学 | Method and device for improving urban domestic sewage treatment performance of ANAMMOX process |
| CN115011585B (en) * | 2022-06-21 | 2024-01-30 | 重庆大学 | Anaerobic ammonia oxidizing bacteria-short-cut nitrifying bacteria co-embedded pellet and preparation method and application thereof |
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