CN111979156A - Microbial composition for high-temperature fermentation treatment of municipal sludge and application thereof - Google Patents
Microbial composition for high-temperature fermentation treatment of municipal sludge and application thereof Download PDFInfo
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- CN111979156A CN111979156A CN202010904847.8A CN202010904847A CN111979156A CN 111979156 A CN111979156 A CN 111979156A CN 202010904847 A CN202010904847 A CN 202010904847A CN 111979156 A CN111979156 A CN 111979156A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a microbial composition for high-temperature fermentation treatment of municipal sludge and application thereof. The composition is added with the high-temperature bacteria for high-temperature fermentation treatment of the municipal sludge, so that the fermentation temperature is 30-40 ℃ higher than that of the traditional sludge compost, auxiliary materials are not required to be added in the fermentation process, the fermentation period can be obviously shortened, the process is simplified, harmlessness and thoroughness are realized, the deodorization can be better realized, and the resource utilization of the municipal sludge can be better realized.
Description
Technical Field
The invention belongs to the technical field of environmental management and resource recycling, and particularly relates to a microbial composition for high-temperature fermentation treatment of municipal sludge and application thereof.
Background
The organic solid waste is not only a great environmental pollution source, but also a huge resource and energy bank. According to measurement and calculation, the urban sludge production in China reaches 7462.43 ten thousand tons in 2020. The 'ten pieces of water' released in 2015 requires that the existing sludge treatment and disposal facilities basically complete standard-reaching transformation before 2017, and the harmless municipal sludge of grade and above is more than 90% before 2020. According to the estimation, the investment of newly-added sludge treatment equipment is estimated to reach 500-600 billion, and the sludge treatment can be large in market, but the sludge treatment equipment also becomes one of the most main non-point source pollution sources. Although the traditional high-temperature compost is mature as a municipal sludge resource treatment technology, the defects of long fermentation period, low fermentation temperature, incomplete harmlessness and the like exist, and the large-scale popularization and application of the high-temperature compost are severely restricted.
Therefore, research and development of a method for treating municipal sludge with short fermentation period, high fermentation temperature, thorough harmlessness and good environmental benefits are needed.
Disclosure of Invention
The invention aims to provide a method for ultrahigh temperature fermentation by microbial biomass energy conversion. The method for treating the municipal sludge has the advantages of short fermentation period, thorough harmlessness, good deodorization and good realization of resource utilization of the municipal sludge.
To this end, the present invention provides, in a first aspect, a microbial composition for high temperature fermentation treatment of municipal sludge, comprising Bacillus subtilis, Bacillus marinus, Actinomyces verruculosa, Thermoactinomyces guangxiensis, Geobacillus thermophilus, Geobacillus thermodenitrificans, Thermocellulolytic bacteria, Thermoascus vinelandii, Geobacillus stearothermophilus, Bacillus licheniformis, Pseudomonas obovani, Saccharomycopora glaucosa, Thermomonospora flexus, Geobacillus thermoglucosinolyticus, and Deutsches.
According to the invention, the microorganism composition is a solid powdery seed material obtained by domesticating or seed culturing or fermentation culturing corresponding strains of bacillus subtilis, bacillus marinus, bacillus pumilus, actinomyces galobotus, actinomyces galbana, geobacillus thermophilus, geobacillus denitrificans, saccharolytic pyrolytic cellulose bacteria, cellulolytic pyrolytic cellulose bacteria, saccharothermophilus, geobacillus stearothermophilus, pseudogracillus obovani, saccharomonas glaucoma, monascus flexuosus, geobacillus thermoglucosides and alzheimer's bacteria according to the fermentation strains, wherein the solid powdery seed material is prepared by culturing bacillus subtilis: marine sediment bacillus: actinomadura verruculosa: guangxi high temperature actinomycetes: geobacillus thermophilus: geobacillus thermodenitrificans: saccharolytic and pyrolytic cellulose bacteria: changbai mountain pyrolytic cellulose bacteria: s. thermophilus: geobacillus stearothermophilus: bacillus pseudogracilis of austria: glaucomatomonas sp: thermophilic single spore bacterium: geobacillus thermoglucosides: heidetella (8-32): (7.3-14.6): (1-4): (1-4): (6.5-13.3): (1-3): (4-8): (3-6): (1-3.5): (0.5-1): (1-2): (1-2.5): (0.5-1): (0.5-1.3): (1-2.5), preferably 32: 14.6: 4: 4: 13.3: 3: 8: 6: 3.5: 1: 2: 2.5: 1: 1.3: 2.5 in mass ratio.
In some specific embodiments of the present invention, the Bacillus subtilis (Bacillus subtilis) has a collection number of CGMCC No. 1.12939;
the preservation number of the marine Bacillus (Bacillus oceanisediminis) is CGMCC 1.10115;
the preservation number of the Actinomadura verrucosa actinomycete (Actinomadura verrucosospora) is CGMCC 4.2116;
the preservation number of the eurystic actinomyces guangxiensis is CGMCC 4.7156;
the Geobacillus thermodenitalis (Geobacillus tepidamans) has a deposit number of ATCC-BAA-942/DSM 16325;
the Geobacillus thermodenitrificans (Geobacillus thermodenitrificans) has a deposit number of ATCC 29492/DSM 465;
the preservation number of the saccharolytic and pyrolytic cellulose bacteria (Caldicellulosriptor saccharolyticus) is CGMCC 1.5183;
the preservation number of the Changbai mountain pyrolytic cellulose bacteria (Caldicellulosriptor changbaiensis) is CGMCC 1.5180;
the deposited number of the Thermococcus thermophilus (Streptomyces thermovulgaris) is ATCC 43125;
the preservation number of the Geobacillus stearothermophilus is CGMCC 1.16087;
the preservation number of the pseudomonas obovani (Pseudomonas auburn) is CGMCC 1.15265(DSM 28556);
the saccharomonas glaucomatosis (Saccharomonospora glauca) has the deposit number DSM 44012;
the preservation number of the high-temperature monascus flexuora is CGMCC 4.2029;
the preservation number of the Geobacillus thermoglucosides (Geobacillus thermoglucosidasius) is CGMCC 1.10851;
the preservation number of the Hidetella (Dietzia maris) is CGMCC 1.6332.
In a second aspect, the present invention provides a method for performing high temperature fermentation treatment on municipal sludge by using the microbial composition of the first aspect, comprising:
step S1, the mixture of the fermentation raw material of the ith period and the fermentation strain of the ith period is fermented for the ith period to obtain the fermentation product of the ith period;
step S2, the fermentation product of the ith period is circularly returned to be used as the fermentation strain;
wherein the fermentation treatment is aerobic fermentation; i is the number of actual fermentation cycles, which is a natural number less than or equal to N; n is the number of the maximum fermentation period, N.gtoreq.1, preferably N.gtoreq.5, and more preferably N.gtoreq.5-7.
According to the method, when i is 1, the fermentation raw material of the ith period is municipal sludge, the fermentation strain of the ith period is bacillus subtilis, marine sediment bacillus, actinomyces verruculosa madura, actinomyces guangxi high temperature, geobacillus thermophilus, geobacillus thermodenitrificans, saccharolytic pyrolytic cellulose bacteria, pyrolysin bacteria, saccharococcus thermophilus, geobacillus stearothermophilus, pseudomonas obovani, saccharomonas glaucosa, thermomonospora flexuosus, geobacillus thermoglucosides and hatzschia strain, and the solid powdery seed material obtained by seed culture of the corresponding strains of the bacillus subtilis: marine sediment bacillus: actinomadura verruculosa: guangxi high temperature actinomycetes: geobacillus thermophilus: geobacillus thermodenitrificans: saccharolytic and pyrolytic cellulose bacteria: changbai mountain pyrolytic cellulose bacteria: s. thermophilus: geobacillus stearothermophilus: bacillus pseudogracilis of austria: glaucomatomonas sp: thermophilic single spore bacterium: geobacillus thermoglucosides: heidetella (8-32): (7.3-14.6): (1-4): (1-4): (6.5-13.3): (1-3): (4-8): (3-6): (1-3.5): (0.5-1): (1-2): (1-2.5): (0.5-1): (0.5-1.3): (1-2.5), preferably 32: 14.6: 4: 4: 13.3: 3: 8: 6: 3.5: 1: 2: 2.5: 1: 1.3: 2.5 in mass ratio;
when i is more than 1 and less than or equal to N, the mixture of the fermentation raw material in the ith period and the fermentation strain in the ith period is a fermentation product in the (i-1) th period;
when i is more than or equal to 1 and less than N, the fermentation product of the ith period is used as the fermentation raw material of the (i + 1) th period;
and when i is equal to N, taking the fermentation product of the ith period as a fermentation strain to be mixed with a fermentation raw material or as a raw bacterium to be used for preparing a soil conditioner or an organic fertilizer.
In some embodiments of the present invention, in step S1, the ventilation amount is controlled to be 0.01-0.1m3/min·m3。
In some embodiments of the invention, in step S1, the fermenting species is added in the form of a solid powdered seed; further preferably, the solid powdered seed material of the microbial composition is added in an amount of 15-100% (w/w), further preferably 30-80% (w/w), based on the total weight of the municipal sludge.
In some embodiments of the invention, in step S1, the microbial composition solid powder seed material has a bacterial density of 2 x 10 or more8CFU/g。
According to the invention, when i is 1, the water content of the fermentation mixed material in the ith period is controlled to be 45-55%; and/or the water content of the fermentation product of the ith period is 36-39%; and/or, when i ═ N, the water content of the fermentation product of the i-th cycle is below 30%.
In some embodiments of the present invention, in step S1, the temperature of the ith cycle of fermentation is 75 ℃ or higher, and the time of the first cycle of fermentation is 7 days; preferably, the fermentation temperature of the 4 th to 7 th days in the fermentation process of the first period is less than or equal to 90 ℃.
According to the invention, the temperature of the 2 nd to 6 th period of fermentation is 75 to 110 ℃, and the time of the 2 nd to 6 th period of fermentation is 30 days; preferably, in the fermentation process of the 2 nd to 6 th periods, the fermentation temperature is 75 to 90 ℃ in 15 days, and the fermentation temperature is more than 90 ℃ in 15 days.
In some embodiments of the invention, during the 2 nd to 6 th cycle of fermentation, stirring is performed every 7 to 10 days; preferably, when the fermentation temperature of the 2 nd to 6 th periods is more than or equal to 90 ℃, the temperature is reduced and the drying speed is accelerated by automatically turning the tank; further preferably, the fermentation speed is increased by controlling the air volume.
In some embodiments of the invention, the fermentation product of the nth cycle is used as a soil amendment substrate.
The invention provides a microbial composition for high-temperature fermentation treatment of municipal sludge. The composition is added with the high-temperature bacteria for high-temperature fermentation treatment of the municipal sludge, so that the fermentation temperature is 30-40 ℃ higher than that of the traditional sludge compost, auxiliary materials are not required to be added in the fermentation process, the fermentation period can be obviously shortened, the process is simplified, harmlessness and thoroughness are realized, the deodorization can be better realized, and the resource utilization of the municipal sludge can be better realized.
Drawings
For the present invention to be readily understood, the following description is made with reference to the accompanying drawings.
FIG. 1 is a graph showing the temperature variation of municipal sludge in different fermentation cycles.
FIG. 2 shows the change of water content of the sludge before and after the fermentation of the municipal sludge in the present invention.
FIG. 3 shows the results of odor detection changes during the fermentation of municipal sludge according to the invention.
Detailed Description
In order that the invention may be readily understood, a more particular description thereof will be rendered by reference to the appended drawings. However, before the invention is described in detail, it is to be understood that this invention is not limited to particular embodiments described. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting.
Unless otherwise defined, all terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention, the preferred methods and materials are now described.
Term (I)
The term "municipal sludge" as used herein refers to sludge produced during operation and maintenance of municipal facilities in and associated with municipal activities. .
The term "recycling" as used herein means that waste is directly used as a raw material or is recycled.
The term "desiccation" as used in the present invention means the separation, evaporation to dryness or removal of water from the waste material by the action of bacteria or high temperature.
The term "ultrahigh temperature fermentation" refers to an aerobic fermentation process with a fermentation temperature of more than or equal to 80 ℃.
The term "mesophilic" as used herein means a fermentation temperature in the range of room temperature to 70 deg.C (inclusive of room temperature, excluding 70 deg.C).
The term "high temperature" as used herein means a fermentation temperature in the range of 70-80 deg.C (including 70 deg.C, excluding 80 deg.C).
Embodiments II
As described above, in the prior art, although the traditional high-temperature composting as a municipal sludge resource treatment technology is mature, the defects of long fermentation period, low fermentation temperature, incomplete harmlessness and the like exist, and the large-scale popularization and application of the high-temperature composting are severely restricted. Therefore, research and development of a method for treating municipal sludge with short fermentation period, high fermentation temperature, thorough harmlessness and good environmental benefits are needed. In view of this, the present inventors have conducted extensive studies on a municipal sludge recycling treatment technology based on high-temperature composting.
The inventor researches and discovers that bacillus subtilis, marine sediment bacillus, actinomyces verruculosa, actinomyces galacicola, geobacillus thermophilus, geobacillus denitrificans, saccharolytic pyrolytic cellulose bacteria, cellulolytic pyrolytic cellulose bacteria, saccharomyces thermophilus, geobacillus stearothermophilus, pseudogracillus obovani, saccharomonas glaucoma, high-temperature monascus flexus, geobacillus thermoglucosides and alzheimer's bacteria are prepared into a microbial composition according to a certain proportion, the composition is added with the high-temperature bacteria for high-temperature fermentation treatment of municipal sludge, so that the fermentation temperature is 30-40 ℃ higher than that of the traditional sludge compost, auxiliary materials are not required to be added in the fermentation process, the fermentation period can be obviously shortened, the process is simplified, harmless and thorough, deodorization can be better realized, and simultaneously the resource utilization of the municipal sludge can be better realized, the present invention was thus obtained.
Accordingly, the present invention relates, in a first aspect, to a microbial composition for high temperature fermentation treatment of municipal sludge, which comprises Bacillus subtilis, Bacillus marinus, Actinomyces verruculosa, Thermoactinomyces guangxiensis, Geobacillus thermophilus, Geobacillus thermodenitrificans, Thermocellulolytic bacteria, Thermococcus lactis, Geobacillus stearothermophilus, Bacillus licheniformis Orbenificans, Saccharomonas glaucopiae, Thermomonospora flexus, Geobacillus thermoglucosinolyticus, and Deutzilla hydnocardiae.
According to the invention, the microorganism composition is a solid powdery seed material obtained by domesticating or seed culturing or fermentation culturing corresponding strains of bacillus subtilis, bacillus marinus, bacillus pumilus, actinomyces galobotus, actinomyces galbana, geobacillus thermophilus, geobacillus denitrificans, saccharolytic pyrolytic cellulose bacteria, cellulolytic pyrolytic cellulose bacteria, saccharothermophilus, geobacillus stearothermophilus, pseudogracillus obovani, saccharomonas glaucoma, monascus flexuosus, geobacillus thermoglucosides and alzheimer's bacteria according to the fermentation strains, wherein the solid powdery seed material is prepared by culturing bacillus subtilis: marine sediment bacillus: actinomadura verruculosa: guangxi high temperature actinomycetes: geobacillus thermophilus: geobacillus thermodenitrificans: saccharolytic and pyrolytic cellulose bacteria: changbai mountain pyrolytic cellulose bacteria: s. thermophilus: geobacillus stearothermophilus: bacillus pseudogracilis of austria: glaucomatomonas sp: thermophilic single spore bacterium: geobacillus thermoglucosides: heidetella (8-32): (7.3-14.6): (1-4): (1-4): (6.5-13.3): (1-3): (4-8): (3-6): (1-3.5): (0.5-1): (1-2): (1-2.5): (0.5-1): (0.5-1.3): (1-2.5), preferably 32: 14.6: 4: 4: 13.3: 3: 8: 6: 3.5: 1: 2: 2.5: 1: 1.3: 2.5, and is also called as a microorganism composition solid powder seed material for high-temperature fermentation treatment of municipal sludge in the invention.
Research results show that the microbial composition formed by adding the high-temperature bacteria is applied to resource utilization of municipal sludge, the fermentation temperature can be 30-40 ℃ higher than that of the traditional sludge compost, auxiliary materials are not required to be added in the fermentation process, the fermentation period can be obviously shortened, the process is simplified, and the harmless effect is enhanced.
In the microbial composition, the preservation number of the Bacillus subtilis is CGMCC No. 1.12939;
the preservation number of the marine Bacillus (Bacillus oceanisediminis) is CGMCC 1.10115;
the preservation number of the Actinomadura verrucosa actinomycete (Actinomadura verrucosospora) is CGMCC 4.2116;
the preservation number of the eurystic actinomyces guangxiensis is CGMCC 4.7156;
the Geobacillus thermodenitalis (Geobacillus tepidamans) has a deposit number of ATCC-BAA-942/DSM 16325;
the Geobacillus thermodenitrificans (Geobacillus thermodenitrificans) has a deposit number of ATCC 29492/DSM 465;
the preservation number of the saccharolytic and pyrolytic cellulose bacteria (Caldicellulosriptor saccharolyticus) is CGMCC 1.5183;
the preservation number of the Changbai mountain pyrolytic cellulose bacteria (Caldicellulosriptor changbaiensis) is CGMCC 1.5180;
the Thermococcus thermophilus (Streptomyces thermovulgaris) has a deposit number ATCC43125(DSM 4749 or CCM 3586);
the preservation number of the Geobacillus stearothermophilus is CGMCC 1.16087;
the preservation number of the pseudomonas obovani (Pseudomonas auburn) is CGMCC 1.15265(DSM 28556);
the saccharomonas glaucomatosis (Saccharomonospora glauca) has the deposit number DSM 44012;
the preservation number of the high-temperature monascus flexuora is CGMCC 4.2029;
the preservation number of the Geobacillus thermoglucosides (Geobacillus thermoglucosidasius) is CGMCC 1.10851;
the preservation number of the Hidetella (Dietzia maris) is CGMCC 1.6332.
Among the above-mentioned fermentation strains, Geobacillus thermodenitrificans (Geobacillus thermonestrificans), Saccharolyticellulose (Caldcellulosriuccharolyticus), and Caldcellulosriumcellulosum changbaiensis are all middle-high temperature bacteria.
Among the above-mentioned fermentation strains, CGMCC strain was purchased from China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center), Geobacillus thermodenitrificans and Saccharococcus thermophilus were purchased from China Industrial Culture Collection Center (China Center of Industrial Culture Collection), and Geobacillus thermodenitrificans and Saccharomonas glaucopiae were purchased from International reagent import and export Logistics Center for biological wind.
The second aspect of the present invention relates to a method for treating municipal sludge by high-temperature fermentation using the microbial composition according to the first aspect of the present invention, which comprises:
step S1, the mixture of the fermentation raw material of the ith period and the fermentation strain of the ith period is fermented for the ith period to obtain the fermentation product of the ith period;
step S2, the fermentation product of the ith period is circularly returned to be used as the fermentation strain;
wherein the fermentation treatment is aerobic fermentation; i is the number of the actual fermentation period, which is a natural number less than or equal to N; n is the number of the maximum fermentation period, N.gtoreq.1, preferably N.gtoreq.5, and more preferably N.gtoreq.5-7.
According to the method, when i is 1, the fermentation raw material of the ith period is municipal sludge, the fermentation strain of the ith period is bacillus subtilis, marine sediment bacillus, actinomyces verruculosa madura, actinomyces guangxi high temperature, geobacillus thermophilus, geobacillus thermodenitrificans, saccharolytic pyrolytic cellulose bacteria, pyrolysin bacteria, saccharococcus thermophilus, geobacillus stearothermophilus, pseudomonas obovani, saccharomonas glaucosa, monascus flexuosus, geobacillus thermoglucosinolate and Haiditius, and the solid powder-shaped seed material obtained by acclimatization or seed culture or fermentation culture of the corresponding strain of the bacillus subtilis: marine sediment bacillus: actinomadura verruculosa: guangxi high temperature actinomycetes: geobacillus thermophilus: geobacillus thermodenitrificans: saccharolytic and pyrolytic cellulose bacteria: changbai mountain pyrolytic cellulose bacteria: s. thermophilus: geobacillus stearothermophilus: bacillus pseudogracilis of austria: glaucomatomonas sp: thermophilic single spore bacterium: geobacillus thermoglucosides: heidetella (8-32): (7.3-14.6): (1-4): (1-4): (6.5-13.3): (1-3): (4-8): (3-6): (1-3.5): (0.5-1): (1-2): (1-2.5): (0.5-1): (0.5-1.3): (1-2.5), preferably 32: 14.6: 4: 4: 13.3: 3: 8: 6: 3.5: 1: 2: 2.5: 1: 1.3: 2.5, also called the microorganism composition solid powder seed material used for the high-temperature fermentation treatment of the municipal sludge in the invention, and is simply called the microorganism composition solid powder seed material of the invention;
in the present invention, the solid powdery inoculum for the fermentation strain is preferably prepared by acclimatization or fermentation culture of a corresponding strain of the fermentation strain. For example, in some instances, the undersize of fermentation products produced during fermentation of the present invention having a particle size of less than 25mm may be employed as a solid powdered seed for the corresponding fermenting species for fermentation.
When i is more than 1 and less than or equal to N, the mixture of the fermentation raw material in the ith period and the fermentation strain in the ith period is a fermentation product in the (i-1) th period;
when i is more than or equal to 1 and less than N, the fermentation product of the ith period is used as the fermentation raw material of the (i + 1) th period;
when i ═ N, the fermentation product of the i-th cycle can be used as a fermentation strain to be mixed with a fermentation raw material (of the next batch) or used as a raw bacterium for preparing a soil conditioner or an organic fertilizer.
It will be understood by those skilled in the art that the "number of highest fermentation cycles, N", refers to the total number of fermentation cycles, and the nth fermentation cycle refers to the last fermentation cycle; the corresponding "number of actual fermentation cycles" refers to the number of sequential fermentation cycles between 1 and N.
It is to be understood that the above steps S1-S2 are representative steps of the fermentation process of the present invention, or are general steps.
In some embodiments of the present invention, in step S1, the ventilation amount is controlled to be 0.01-0.1m3/min·m3。
In some embodiments of the invention, in step S1, the fermenting species is added in the form of a solid powdered seed; further preferably, the solid powdered seed material of the microbial composition is added in an amount of 15-100% (w/w), further preferably 30-80% (w/w), based on the total weight of the municipal sludge.
In some embodiments of the invention, in step S1, the microbial composition solid powder seed material has a bacterial density of 2 x 10 or more8CFU/g. This is understood to mean that the microbial composition solid powdered seed material has a total bacterial density of 2X 10 or more8CFU/g, and the density of the solid powder seed material of each constituent strain in the solid powder seed material of the microbial composition is not less than 2 x 108CFU/g。
As the bacteria used in the fermentation process of the invention are aerobic thermophilic bacteria, a large amount of heat is generated in the garbage fermentation process, so that the temperature of the fermentation tank (in a fermentation tank or a fermentation tunnel) is increased, and the temperature can be reduced by adjusting the ventilation quantity or adjusting the ventilation quantity and turning over the tank, preferably by adjusting the ventilation quantity, thereby realizing the temperature control. In some embodiments of the invention, the temperature of the fermentation is 40-110 ℃, preferably 42.5-95 ℃.
In some embodiments of the invention, the total fermentation time of the fermentation process is 1 to 45 days, preferably 1 to 37 days.
It is readily understood that the total fermentation time of the fermentation process refers to the sum of the time of the individual fermentation cycles. In the invention, the termination time of the fermentation period can be determined according to the moisture content, the temperature and the color of the fermentation product in each fermentation period. For example, when i is 1, controlling the water content of the fermentation mixed material of the ith period to be 45-55%; when the water content of the fermentation product in the ith period is lower than 36-39%, turning and throwing can be carried out; when the water content of the fermentation product in the ith cycle is below 30% (w/w), the fermentation can be stopped.
In the present invention, the fermentation of the next cycle is generally carried out by turning over.
In some embodiments of the present invention, in step S1, the temperature of the ith cycle of fermentation is 75 ℃ or higher, and the time of the first cycle of fermentation is 7 days; preferably, the fermentation temperature of the 4 th to 7 th days in the fermentation process of the first period is less than or equal to 90 ℃.
According to the invention, the temperature of the 2 nd to 6 th period of fermentation is 75 to 110 ℃, and the time of the 2 nd to 6 th period of fermentation is 30 days; preferably, in the fermentation process of the 2 nd to 6 th periods, the fermentation temperature is 75 to 90 ℃ in 15 days, and the fermentation temperature is more than 90 ℃ in 15 days.
In some embodiments of the invention, during the 2 nd to 6 th cycle of fermentation, stirring is performed every 7 to 10 days; preferably, when the fermentation temperature of the 2 nd to 6 th periods is more than or equal to 90 ℃, the temperature is reduced and the drying speed is accelerated by automatically turning the tank; further preferably, the fermentation speed is increased by controlling the air volume.
In some embodiments of the invention, the fermentation product of the nth cycle is used as a soil amendment substrate.
The organic matter monitoring method and apparatus of the present invention are not particularly limited, and may be, for example, methods and apparatuses for organic matter detection that are conventional in the art.
The method and apparatus for detecting the density of bacteria in the present invention are not particularly limited, and may be, for example, those conventionally used in the art.
The method and equipment for detecting the water content in the present invention are not particularly limited, and may be, for example, methods and equipment commonly used in the art, and may be commercially available or commercially available on-line monitoring devices for water content.
The temperature detection method and equipment in the present invention are not particularly limited, and may be, for example, a temperature detection method and equipment commonly used in the art, and may be a commercially available or commercially available temperature on-line monitoring device.
Organic matters, total nitrogen, phosphorus, potassium, water, pH value, total arsenic, total mercury, total lead, total cadmium, total chromium, fecal coliform flora number, ascarid egg death rate and the like are detected according to organic fertilizer (NY525-2012) which is the agricultural industry standard of the people's republic of China.
Example III
The present invention will be specifically described below with reference to specific examples. The experimental methods described below are, unless otherwise specified, all routine laboratory procedures. The experimental materials described below, unless otherwise specified, are commercially available.
Example 1:
(1) the solid powder-shaped seed material obtained by domesticating or seed culturing or fermenting and culturing corresponding strains of bacillus subtilis, marine sediment bacillus, verruca madura actinomyces, eurytococcus guangxi, geobacillus thermophilus, geobacillus denitrificans, saccharolytic pyrolytic cellulose bacteria, Changbai mountain pyrolytic cellulose bacteria, saccharococcus thermophilus, geobacillus stearothermophilus, pseudogracillus obovani, glaucomonas glaucomatosus, high-temperature monad flexus, geobacillus thermoglucosides and heidinierella according to the fermentation strains is prepared from bacillus subtilis: marine sediment bacillus: actinomadura verruculosa: guangxi high temperature actinomycetes: geobacillus thermophilus: geobacillus thermodenitrificans: saccharolytic and pyrolytic cellulose bacteria: changbai mountain pyrolytic cellulose bacteria: s. thermophilus: geobacillus stearothermophilus: bacillus pseudogracilis of austria: glaucomatomonas sp: thermophilic single spore bacterium: geobacillus thermoglucosides: 32 parts of heidinieria: 14.6: 4: 4: 13.3: 3: 8: 6: 3.5: 1: 2: 2.5: 1: 1.3: 2.5, and the solid powder type seed material of the microbial composition for the high-temperature fermentation treatment of the municipal sludge is prepared.
(2) Mixing the metered municipal sludge and the solid powdery microbial composition seed material in proportion (based on the total weight of the municipal sludge, the addition amount of the solid powdery microbial composition seed material is 30-80 wt%), piling the mixture to the top of a fermentation tank, distributing an air supply pipe at the bottom of the fermentation tank, supplying air into the pile through a fan to ensure oxygen required by a high-temperature aerobic fermentation process, and controlling the ventilation amount to be 0.01-0.1m3/min·m3. And after 5-7 days of the first period, when the water content of the fermentation product is lower than 36% -39%, turning over the fermentation tank, and transferring the mixed material in the fermentation tank to another fermentation tank by using a loading vehicle to continue fermentation in the next period.
(3) After 5-7 periods of fermentation, when the water content of the fermentation product is lower than 30%, stopping fermentation, and completing the whole fermentation process.
(4) The product after fermentation can be used as original bacteria to be mixed with the original material again, and new sludge is circularly treated.
Due to the high fermentation temperature, the fermentation days at the temperature of more than 75 ℃ exceed more than 30 days in the whole fermentation period of 37 days (see figure 1), the high-temperature compound thermophilic bacteria proliferates and activates under the high-temperature aerobic condition at the temperature of more than 80 ℃, the water evaporation is rapid, the water content of the fermentation product is low, and the reduction is obvious (see figure 2). No auxiliary materials are added in the whole fermentation process, no malodorous gas is generated, most of the malodorous gas is ammonia gas, and the odor of the new malodorous sludge is immediately reduced after being mixed with the original bacteria, as shown in figure 3. Meanwhile, because the fermentation temperature is high, pathogenic bacteria and pathogenic microorganisms can be fully killed, and no mosquitoes or flies exist. The analysis results of the components of the fermentation product in the embodiment are shown in table 1, and the detection results show that the components of the fermentation product in the embodiment meet the national agricultural organic fertilizer standard NY 525-2012. Through a germination test, the fermentation product can be thoroughly decomposed, has good fertilizer efficiency, can be used as a soil conditioner substrate, and realizes resource utilization.
TABLE 1 analysis of fermentation product composition
It should be noted that the above-mentioned embodiments are only for explaining the present invention, and do not constitute any limitation to the present invention. The present invention has been described with reference to exemplary embodiments, but the words which have been used herein are words of description and illustration, rather than words of limitation. The invention can be modified, as prescribed, within the scope of the claims and without departing from the scope and spirit of the invention. Although the invention has been described herein with reference to particular means, materials and embodiments, the invention is not intended to be limited to the particulars disclosed herein, but rather extends to all other methods and applications having the same functionality.
Claims (11)
1. A microbial composition for high-temperature fermentation treatment of municipal sludge comprises bacillus subtilis, bacillus marinum, actinomyces madurae, actinomyces guangxi, bacillus geothermus thermophilus, geobacillus thermophilus denitrificans, saccharolytic pyrolytic cellulose bacteria, pyrolcellulose bacteria of Changbai mountain, saccharococcus thermophilus, geobacillus stearothermophilus, bacillus pseudogracilis, saccharomonas glaucoma, thermomonospora flexuosus, geobacillus thermoglucosides and bordetella haiti.
2. The microbial composition according to claim 1, wherein the microbial composition is a solid powdery seed material obtained by acclimatizing or seed-culturing or fermentatively culturing a corresponding strain of bacillus subtilis, bacillus marinum, actinomyces verruculosa, actinomyces guangxi, geobacillus thermophilus, geobacillus denitrificans, saccharolytic pyrolytic cellulose bacteria, cellulolytic pyrolytic cellulose bacteria, saccharococcus thermophilus, geobacillus stearothermophilus, bacillus pseudogracilis, saccharomonas glaucoma, thermomonospora flexus, geobacillus thermoglucosides, and dietzia according to the fermentation strain: marine sediment bacillus: actinomadura verruculosa: guangxi high temperature actinomycetes: geobacillus thermophilus: geobacillus thermodenitrificans: saccharolytic and pyrolytic cellulose bacteria: changbai mountain pyrolytic cellulose bacteria: s. thermophilus: geobacillus stearothermophilus: bacillus pseudogracilis of austria: glaucomatomonas sp: thermophilic single spore bacterium: geobacillus thermoglucosides: heidetella (8-32): (7.3-14.6): (1-4): (1-4): (6.5-13.3): (1-3): (4-8): (3-6): (1-3.5): (0.5-1): (1-2): (1-2.5): (0.5-1): (0.5-1.3): (1-2.5), preferably 32: 14.6: 4: 4: 13.3: 3: 8: 6: 3.5: 1: 2: 2.5: 1: 1.3: 2.5 in mass ratio.
3. The microbial composition of claim 1 or 2,
the preservation number of the Bacillus subtilis is CGMCC No. 1.12939;
the preservation number of the marine Bacillus (Bacillus oceanisediminis) is CGMCC 1.10115;
the preservation number of the Actinomadura verrucosa actinomycete (Actinomadura verrucosospora) is CGMCC 4.2116;
the preservation number of the eurystic actinomyces guangxiensis is CGMCC 4.7156;
the Geobacillus thermodenitalis (Geobacillus tepidamans) has a deposit number of ATCC-BAA-942/DSM 16325;
the Geobacillus thermodenitrificans (Geobacillus thermodenitrificans) has a deposit number of ATCC 29492/DSM 465;
the preservation number of the saccharolytic and pyrolytic cellulose bacteria (Caldicellulosriptor saccharolyticus) is CGMCC 1.5183;
the preservation number of the Changbai mountain pyrolytic cellulose bacteria (Caldicellulosriptor changbaiensis) is CGMCC 1.5180;
the deposited number of the Thermococcus thermophilus (Streptomyces thermovulgaris) is ATCC 43125;
the preservation number of the Geobacillus stearothermophilus is CGMCC 1.16087;
the preservation number of the pseudomonas obovani (Pseudomonas auburn) is CGMCC 1.15265(DSM 28556);
the saccharomonas glaucomatosis (Saccharomonospora glauca) has the deposit number DSM 44012;
the preservation number of the high-temperature monascus flexuora is CGMCC 4.2029;
the preservation number of the Geobacillus thermoglucosides (Geobacillus thermoglucosidasius) is CGMCC 1.10851;
the preservation number of the Hidetella (Dietzia maris) is CGMCC 1.6332.
4. A method for treating municipal sludge by high-temperature fermentation using the microbial composition according to any one of claims 1 to 3, which comprises:
step S1, the mixture of the fermentation raw material of the ith period and the fermentation strain of the ith period is fermented for the ith period to obtain the fermentation product of the ith period;
step S2, the fermentation product of the ith period is circularly returned to be used as the fermentation strain;
wherein the fermentation treatment is aerobic fermentation; i is the number of actual fermentation cycles, which is a natural number less than or equal to N; n is the number of the maximum fermentation period, N.gtoreq.1, preferably N.gtoreq.5, and more preferably N.gtoreq.5-7.
5. The method of claim 3,
when i is 1, the fermentation raw material of the ith period is municipal sludge, the fermentation strain of the ith period is bacillus subtilis, marine sediment bacillus, actinomyces verruculosa, actinomyces guangxi, geobacillus thermophilus denitrificans, saccharolytic pyrolytic cellulose bacteria, pyrolysin bacteria of Changbai mountain, saccharococcus thermophilus, geobacillus stearothermophilus, pseudogracillus obovatus, saccharomonas glaucoma, thermomonospora flexuosus, geobacillus thermoglucosides and Haidiella according to the solid powdery seed material obtained by seed culture of corresponding strains of the fermentation strains of bacillus subtilis: marine sediment bacillus: actinomadura verruculosa: guangxi high temperature actinomycetes: geobacillus thermophilus: geobacillus thermodenitrificans: saccharolytic and pyrolytic cellulose bacteria: changbai mountain pyrolytic cellulose bacteria: s. thermophilus: geobacillus stearothermophilus: bacillus pseudogracilis of austria: glaucomatomonas sp: thermophilic single spore bacterium: geobacillus thermoglucosides: heidetella (8-32): (7.3-14.6): (1-4): (1-4): (6.5-13.3): (1-3): (4-8): (3-6): (1-3.5): (0.5-1): (1-2): (1-2.5): (0.5-1): (0.5-1.3): (1-2.5), preferably 32: 14.6: 4: 4: 13.3: 3: 8: 6: 3.5: 1: 2: 2.5: 1: 1.3: 2.5 in mass ratio;
when i is more than 1 and less than or equal to N, the mixture of the fermentation raw material in the ith period and the fermentation strain in the ith period is a fermentation product in the (i-1) th period;
when i is more than or equal to 1 and less than N, the fermentation product of the ith period is used as the fermentation raw material of the (i + 1) th period;
and when i is equal to N, taking the fermentation product of the ith period as a fermentation strain to be mixed with a fermentation raw material or as a raw bacterium to be used for preparing a soil conditioner or an organic fertilizer.
6. The method according to claim 4 or 5, wherein the ventilation amount is controlled to 0.01-0.1m in step S13/min·m3(ii) a And/or, the fermentation strain is added in the form of solid powdery seed material; further preferably, the solid powdered seed material of the microbial composition is added in an amount of 15-100% (w/w), further preferably 30-80% (w/w), based on the total weight of the municipal sludge; and/or the bacterial density of the solid powdery seed material of the microbial composition is more than or equal to 2 multiplied by 108CFU/g。
7. The method according to any one of claims 4 to 6, characterized in that when i is 1, the water content of the fermentation batch of the i-th cycle is controlled to 45% -55%; and/or the water content of the fermentation product of the ith period is 36-39%; and/or, when i ═ N, the water content of the fermentation product of the i-th cycle is below 30%.
8. The method according to any one of claims 4 to 7, wherein in step S1, the temperature of the fermentation in the ith cycle is 75 ℃ or more, and the time of the fermentation in the first cycle is 7 days; preferably, the fermentation temperature of the 4 th to 7 th days in the fermentation process of the first period is less than or equal to 90 ℃.
9. The method according to any one of claims 4 to 8, wherein the temperature of the 2 nd to 6 th cycle of fermentation is 75 to 110 ℃ and the time of the 2 nd to 6 th cycle of fermentation is 30 days; preferably, in the fermentation process of the 2 nd to 6 th periods, the fermentation temperature is 75 to 90 ℃ in 15 days, and the fermentation temperature is more than 90 ℃ in 15 days.
10. The method of claim 9, wherein during the 2 nd to 6 th cycle of fermentation, stirring is performed every 7 to 10 days; preferably, when the fermentation temperature of the 2 nd to 6 th periods is more than or equal to 90 ℃, the temperature is reduced and the drying speed is accelerated by automatically turning the tank; further preferably, the fermentation speed is increased by controlling the air volume.
11. The method according to any one of claims 1 to 10, wherein the fermentation product of the nth cycle is used as a soil amendment substrate.
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