CN111575218A - Composite microbial preparation with function of degrading bioflocculant and preparation method thereof - Google Patents
Composite microbial preparation with function of degrading bioflocculant and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 230000000813 microbial effect Effects 0.000 title claims abstract description 25
- 239000002131 composite material Substances 0.000 title claims abstract description 23
- 230000000593 degrading effect Effects 0.000 title claims description 6
- 239000010802 sludge Substances 0.000 claims abstract description 100
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 20
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 20
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 18
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 18
- 230000015556 catabolic process Effects 0.000 claims abstract description 13
- 238000006731 degradation reaction Methods 0.000 claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 9
- 229960001031 glucose Drugs 0.000 claims abstract description 9
- 108010059892 Cellulase Proteins 0.000 claims abstract description 8
- 229940106157 cellulase Drugs 0.000 claims abstract description 8
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- 229920002678 cellulose Polymers 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
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- 238000000034 method Methods 0.000 claims description 18
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- 238000001694 spray drying Methods 0.000 claims description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 229940099596 manganese sulfate Drugs 0.000 claims description 4
- 239000011702 manganese sulphate Substances 0.000 claims description 4
- 235000007079 manganese sulphate Nutrition 0.000 claims description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 229920000858 Cyclodextrin Polymers 0.000 claims description 2
- 239000001116 FEMA 4028 Substances 0.000 claims description 2
- 241000233866 Fungi Species 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 230000003213 activating effect Effects 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 2
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 2
- 229960004853 betadex Drugs 0.000 claims description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 2
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 229920000136 polysorbate Polymers 0.000 claims description 2
- 239000001103 potassium chloride Substances 0.000 claims description 2
- 235000011164 potassium chloride Nutrition 0.000 claims description 2
- 239000001632 sodium acetate Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 2
- 239000012137 tryptone Substances 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 abstract description 63
- 230000004151 fermentation Effects 0.000 abstract description 63
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 229940088598 enzyme Drugs 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 29
- 241000196324 Embryophyta Species 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
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- 230000008020 evaporation Effects 0.000 description 7
- 238000007405 data analysis Methods 0.000 description 6
- 238000000354 decomposition reaction Methods 0.000 description 5
- 239000008394 flocculating agent Substances 0.000 description 5
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- 238000006297 dehydration reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 244000000010 microbial pathogen Species 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 206010029350 Neurotoxicity Diseases 0.000 description 1
- 206010044221 Toxic encephalopathy Diseases 0.000 description 1
- AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013400 design of experiment Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 150000002505 iron Chemical class 0.000 description 1
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- 231100000228 neurotoxicity Toxicity 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
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- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses a composite microbial preparation with a degradable biological flocculant, which comprises the following components in parts by weight: 30 parts of Bacillus coagulans (strain number: CGMCC1.10302), 20 parts of Bacillus subtilis (strain number: CGMCC 1.15792), 10 parts of Cellulase (cellulose, 8000U/g) and 140 parts of anhydrous glucose (Amylaceae). The invention successfully develops the composite microbial preparation with the degradation biological flocculant by screening the high-performance spore bacteria and the enzyme preparation and carrying out scientific compatibility aiming at the characteristics of the biological flocculant, and can realize the rapid increase of the sludge fermentation temperature, kill harmful microorganisms in the sludge and rapidly decompose the sludge under the normal high-temperature sludge fermentation production management condition.
Description
Technical Field
The invention relates to the technical field of environmental protection, in particular to a composite microbial preparation with a biodegradable bioflocculant and a preparation method thereof.
Background
The flocculant is widely applied to the fields of water supply, industrial wastewater, municipal sewage and sludge dehydration, fermentation industry post-treatment food industry and the like. Traditional flocculants are inorganic flocculants and synthetic organic polymeric flocculants. The inorganic flocculant is mainly two systems of iron salt and aluminum salt, and the organic polymer flocculant is mainly polyacrylamide and derivatives thereof. At present, polyacrylamide is used more, and has the characteristics of small dosage and high flocculation speed, but the application range of the polyacrylamide is limited because the monomers for synthesizing the polymers have strong neurotoxicity and strong 'three-cause' effect.
The Microbial flocculant (MBF) is a novel water treatment agent with biodegradability and safety, which is obtained by fermentation, extraction and refining of microorganisms by utilizing biotechnology. Compared with the traditional flocculating agent, the biological flocculating agent has the advantages of easy microbial degradation, no toxicity, no harm, high safety, high efficiency, no secondary pollution, wide application range and the like.
But the water storage capacity of the biological flocculant is very strong, which brings great trouble to the subsequent treatment of the sludge, especially brings inconvenience to the high-temperature fermentation and dehydration of the sludge, thereby bringing great resistance to the resource recycling of the sludge.
Disclosure of Invention
In order to overcome the defects, the invention provides a composite microbial preparation with a biodegradable bioflocculant and a preparation method thereof, which can improve the high-temperature fermentation quality of sludge and accelerate the resource utilization of the sludge on the basis of good high-temperature fermentation management measures of the sludge.
The invention has the beneficial effects that: the invention successfully develops the composite microbial preparation with the degradation biological flocculant by screening the high-performance spore bacteria and the enzyme preparation and carrying out scientific compatibility aiming at the characteristics of the biological flocculant, and can realize the effects of quickly increasing the fermentation temperature of sludge, killing harmful microorganisms in the sludge and quickly decomposing the sludge through fermentation under the normal high-temperature sludge fermentation production management condition.
Detailed Description
The composite microbial preparation with the function of degrading the bioflocculant is characterized by comprising the following components in parts by mass: 30 parts of Bacillus coagulans (strain number: CGMCC1.10302), 20 parts of Bacillus subtilis (strain number: CGMCC 1.15792), 10 parts of Cellulase (cellulose) and 140 parts of anhydrous glucose (Amylaceum).
The preparation method of the composite microbial preparation with the degradation bioflocculant comprises the following steps:
step 1, preparing a culture medium:
preparation of culture medium A: firstly, heating 4.5 parts of tryptone, 4 parts of beef extract, 2 parts of yeast powder, 1 part of glucose, 1 part of sodium acetate, 2 parts of light calcium carbonate, 0.4 part of ammonium citrate, 800.2 parts of tween, 0.1 part of potassium chloride, 0.1 part of magnesium sulfate, 0.1 part of manganese sulfate and 100 parts of distilled water to 55-70 ℃, and stirring to dissolve all the raw materials; sterilizing at 121 deg.C and 0.15MPa for 30 min, and cooling to 37 deg.C to obtain sterilized culture medium A;
b, preparation of a culture medium: firstly, heating 1.5 parts of peptone, 4 parts of glucose, 2 parts of yeast extract, 0.02 part of dipotassium hydrogen phosphate, 0.02 part of manganese sulfate, 0.01 part of sodium chloride and 100 parts of distilled water to 55-70 ℃, and stirring to dissolve all raw materials; then, adjusting the pH value to 7.0-7.2 by using 20% sodium hydroxide solution to complete the batching; sterilizing at 121 ℃ and 0.15MPa for 30 minutes, and cooling to 37-39 ℃ to obtain a sterilized culture medium B;
step 2, activating and expanding culture of strains:
inoculating the strictly screened and refrigerated bacillus coagulans to 100ml of the sterilized A culture medium for primary shaker activation at the temperature of 37-39 ℃, detecting after 24 hours, completely inoculating 100ml of the bacteria liquid to 5000ml of the sterilized A culture medium for secondary shaker activation after the bacteria liquid reaches the standard, and inoculating the bacteria liquid to the sterilized A culture medium for shaker enlarged culture for 48 hours after the bacteria liquid reaches the standard after 24 hours of detection;
inoculating the bacillus subtilis which is strictly screened and refrigerated to 100ml of the sterilized B culture medium, performing primary shaking table activation at the temperature of 37-39 ℃, detecting after 24 hours, inoculating all 100ml of the bacillus subtilis to 5000ml of the sterilized B culture medium after the bacillus subtilis reaches the standard, performing secondary shaking table activation at the temperature of 37-39 ℃, and inoculating the bacillus subtilis to the sterilized B culture medium after the bacillus subtilis reaches the standard after 24 hours, and performing shaking table expansion culture for 24 hours;
cellulase was purchased from Shandong Zhuo Hua Biotech, Inc.;
step 3, collecting bacterial sludge:
centrifuging the fermented bacillus coagulans liquid by using a centrifugal machine, and collecting bacterial sludge;
the method for collecting bacterial sludge of the bacillus subtilis is the same as that of the bacillus coagulans;
step 4, drying the bacterial sludge:
spray drying the bacillus coagulans bacterial mud collected in the step 3 by a spray drying treatment process to prepare the bacillus coagulans bacterial mud, which comprises the following specific steps: a. adding 15% of beta-cyclodextrin into the bacterial sludge, and uniformly mixing; b. the parameters of the spray dryer were set as follows: carrying out spray drying to prepare powder at the outlet temperature of 75-78 ℃, the inlet temperature of 125-128 ℃ and the feeding speed of 600 ml/h; c. the prepared fungus powder is stored in a sealed environment at-4 ℃;
the bacterial sludge spray drying method of the bacillus subtilis is the same as that of the bacillus coagulans;
step 5, preparing a finished product:
taking the bacterial powder prepared in the step 4, wherein 30 parts of bacillus coagulans and 20 parts of bacillus subtilis, 10 parts of cellulase and 140 parts of anhydrous glucose are uniformly mixed and bagged in an aseptic state, and the number of viable bacteria is 2 × 1010cfu/g, obtaining a composite microbial preparation with a degradation bioflocculant.
The invention has the following action principle: in the initial stage of high-temperature fermentation of sludge, enzymes metabolized by carbon sources and nitrogen sources contained in the bioflocculant can be preferentially utilized by growth and propagation of bacillus coagulans and bacillus subtilis, so that the bioflocculant can be rapidly decomposed, cellulose and hemicellulose are degraded by cellulase, the sludge can rapidly reach the high-temperature fermentation process, pathogenic microorganisms in the sludge can be effectively killed, organic nutrient substances specific to the sludge are kept, and sludge fermentation decomposed substances with high utilization value are produced. The effects of the present invention will be described in detail below.
1. Experimental Material
1) Test site: a first sewage plant of Yanjin initial water affairs GmbH and a second sewage plant of Yanjin initial water affairs GmbH; a first sewage plant of the tunnel real-time water works and a second sewage plant of the tunnel real-time water works.
2) Experimental materials: sludge treated by the biological flocculant, other auxiliary materials and the like.
3) A degradation agent: the invention relates to a composite microbial preparation capable of degrading a bioflocculant.
4) The addition amount is as follows: 200-500 g of the composite microbial agent with the function of degrading the biological flocculant is added into 1 cubic meter of sludge raw material.
2. Design of experiments
The sludge raw materials with the adjusted water and carbon-nitrogen ratio were set as a control group (without adding the composite microbial preparation with a biodegradable bioflocculant of the present invention), the sludge raw materials with the adjusted water and carbon-nitrogen ratio were set as a test group (with adding different qualities of the composite microbial preparation with a biodegradable bioflocculant of the present invention), and the test group was added with an example of the composite microbial degradation agent (see table 1). Wherein, the test group is three treatment groups, test group 1: adding 200g of composite microbial degradation agent according to 1 cubic volume of sludge raw material, and performing a test group 2: adding 300g of composite microbial degradation agent according to 1 cubic volume of sludge raw material, test group 3: 500g of composite microbial degradation agent is added according to 1 cubic volume of sludge raw material. The experimental group examples were obtained according to the raw material formulations shown in table 1 and the process steps described in the present invention.
TABLE 1 examples
3. Experimental methods
1) The sludge and the auxiliary materials are uniformly mixed, so that the water content of the raw materials is controlled to be 55-60%, and the carbon-nitrogen ratio (C/N) of the raw materials is 25-30: 1. the composite microbial preparation with the degradation biological flocculant is uniformly mixed according to the proportion and piled into a strip pile with the height of 120 cm and the width of 100 cm.
2) Temperature detection and turning:
monitoring the central temperature of the strip stacks at any time, turning and throwing for the first time when the central temperature exceeds 60 ℃, turning and throwing once every other day, visually judging the speed of the sludge fermentation process by measuring the central temperature of the strip stacks and the moisture content of the sludge on the 3 rd, 5 th, 7 th, 15 th and 20 th days of sludge fermentation, and marking that the sludge fermentation and decomposition process is finished when the central temperature of the sludge strip stacks is measured to be below 40 ℃, the temperature is not increased after turning and throwing, and the moisture content of the sludge is below 35%.
3) The sludge stack is turned over after the temperature reaches 60 ℃ and is maintained for 48 hours, so that the aim of effectively killing harmful pathogenic microorganisms, weed seeds and the like in the sludge through harmless treatment is fulfilled.
4) And (3) moisture content detection standard: the water content of the sludge is determined according to GBT 24602-2009.
5) And comparing and analyzing the sludge fermentation temperature and the water content of the experimental group and the control group.
4. Data processing and analysis
The data statistical method adopts Excel software to process basic data, adopts SPSS software version 17.0 to carry out variance analysis on the data, and shows that the difference is obvious when P is less than 0.05, and the result is expressed by 'mean +/-standard deviation'.
5. Results and analysis
Experiment one: sludge fermentation test of first sewage plant of Yanjin initiatives Water works Co., Ltd
Sludge fermentation process temperature determination (see table 2): data analysis shows that the temperature of all test groups before 15 days of sludge fermentation is significantly higher than that of a control group (P is less than 0.05), and the control group has no obvious temperature rise process, so that the initial temperature rise of the test groups in the sludge fermentation is significantly better than that of the control group.
TABLE 2 center temperature (. degree. C.) for different days of sludge Bar Stack fermentation
Note: the different lower case letters in the upper right corner of the same row indicate significant differences in mean (P < 0.05), as shown in the table below.
Determination of water content during sludge fermentation (see table 3): data analysis shows that the dehydration speed of sludge fermentation of all test groups is obviously higher than that of a control group (P is less than 0.05), and the water evaporation of the sludge of the control group is slow, so that the sludge fermentation and decomposition process of the test groups is obviously better than that of the control group.
Table 3 moisture content (%) -of sludge windrow fermentation for different days
Experiment two: sludge fermentation test of second sewage plant of Yanjin initiatives Water works Ltd
Sludge fermentation process temperature determination (see table 4): data analysis shows that the temperature of all test groups before 15 days of sludge fermentation is significantly higher than that of a control group (P is less than 0.05), and the sludge fermentation of the control group has no obvious temperature rise process, so that the initial temperature rise of the test groups in the sludge fermentation is significantly better than that of the control group.
TABLE 4 center temperature (. degree.C.) of sludge Bar Stack fermentation for different days
Determination of water content during sludge fermentation (see table 5): the evaporation of the sludge fermentation water content of all the test groups is obviously faster than that of the control group (P is less than 0.05), and the evaporation of the sludge water content of the control group is slow, so that the sludge fermentation and decomposition process of the test groups is obviously better than that of the control group.
Table 5 moisture content (%) -of sludge Bar Stack fermentation on different days
Experiment three: sludge fermentation test of first sewage plant of tunnel-level practical Water works Co., Ltd
Sludge fermentation process temperature determination (see table 6): the data analysis shows that the temperature of the test group 15 days before the sludge fermentation is significantly higher than that of the control group (P is less than 0.05), and the control group has no obvious temperature rise process, so that the initial temperature rise of the test group in the sludge fermentation is significantly better than that of the control group.
TABLE 6 center temperature (. degree.C.) of sludge Bar Stack fermentation for different days
Determination of water content during sludge fermentation (see table 7): the evaporation of the fermentation water content of the sludge of the test group is obviously faster than that of the control group (P is less than 0.05), and the sludge fermentation dehydration speed of the control group is slow, so that the fermentation and decomposition process of the sludge of the test group is obviously better than that of the control group.
Table 7 moisture content (%) -of sludge Bar Stack fermentation on different days
Experiment four: sludge fermentation test in second sewage plant of tunnel-level practical Water works Ltd
Sludge fermentation process temperature determination (see table 8): data analysis shows that the temperature of the test group 15 days before sludge fermentation is significantly higher than that of a control group (P is less than 0.05), and the control group has no obvious temperature rise process, so that the initial temperature rise of the test group in sludge fermentation is significantly better than that of the control group.
TABLE 8 center temperature (. degree.C.) for different days of sludge Bar Stack fermentation
Determination of water content during sludge fermentation (see table 9): the evaporation of the fermentation water content of the sludge in the test group is obviously faster than that in the control group (P is less than 0.05), while the evaporation of the fermentation water content of the sludge in the control group is slow, so that the fermentation and decomposition process of the sludge in the test group is obviously better than that in the control group.
Table 9 moisture content (%) -of sludge Bar Stack fermentation for different days
6. Conclusion of the experiment
The data analysis of the first experiment, the second experiment, the third experiment and the fourth experiment shows that: the initial temperature rise speed and sludge dewatering speed of the test group at the sludge fermentation stage are both obviously higher than those of the control group; the fermentation process and the rotten quality of the sludge of the test group are obviously superior to those of the control group.
In conclusion, the composite microbial preparation with the degradation bioflocculant can rapidly raise the temperature of the initial stage of sludge fermentation and accelerate the evaporation rate of water in the sludge fermentation process under the normal conditions of sludge fermentation production management, and can effectively kill pathogenic microorganisms and weed seeds in sludge, so that the effects of improving the sludge fermentation maturity quality and accelerating the sludge fermentation process are achieved.
Claims (2)
1. The composite microbial preparation with the function of degrading the bioflocculant is characterized by comprising the following components in parts by mass: 30 parts of Bacillus coagulans (strain number: CGMCC1.10302), 20 parts of Bacillus subtilis (strain number: CGMCC 1.15792), 10 parts of Cellulase (cellulose, 8000U/g) and 140 parts of anhydrous glucose (Amylaceae).
2. A method for preparing a composite microbial preparation having a biodegradable bioflocculant according to claim 1, comprising the steps of:
step 1, preparing a culture medium:
preparation of culture medium A: firstly, heating 4.5 parts of tryptone, 4 parts of beef extract, 2 parts of yeast powder, 1 part of glucose, 1 part of sodium acetate, 2 parts of light calcium carbonate, 0.4 part of ammonium citrate, 800.2 parts of tween, 0.1 part of potassium chloride, 0.1 part of magnesium sulfate, 0.1 part of manganese sulfate and 100 parts of distilled water to 55-70 ℃, and stirring to dissolve all the raw materials; sterilizing at 121 deg.C and 0.15MPa for 30 min, and cooling to 37 deg.C to obtain sterilized culture medium A;
b, preparation of a culture medium: firstly, heating 1.5 parts of peptone, 4 parts of glucose, 2 parts of yeast extract, 0.02 part of dipotassium hydrogen phosphate, 0.02 part of manganese sulfate, 0.01 part of sodium chloride and 100 parts of distilled water to 55-70 ℃, and stirring to dissolve all raw materials; then, adjusting the pH value to 7.0-7.2 by using 20% sodium hydroxide solution to complete the batching; sterilizing at 121 ℃ and 0.15MPa for 30 minutes, and cooling to 37-39 ℃ to obtain a sterilized culture medium B;
step 2, activating and expanding culture of strains:
inoculating the strictly screened and refrigerated bacillus coagulans to 100ml of the sterilized A culture medium for primary shaker activation at the temperature of 37-39 ℃, detecting after 24 hours, completely inoculating 100ml of the bacteria liquid to 5000ml of the sterilized A culture medium for secondary shaker activation after the bacteria liquid reaches the standard, and inoculating the bacteria liquid to the sterilized A culture medium for shaker enlarged culture for 48 hours after the bacteria liquid reaches the standard after 24 hours of detection;
inoculating the bacillus subtilis which is strictly screened and refrigerated to 100ml of the sterilized B culture medium, performing primary shaking table activation at the temperature of 37-39 ℃, detecting after 24 hours, inoculating all 100ml of the bacillus subtilis to 5000ml of the sterilized B culture medium after the bacillus subtilis reaches the standard, performing secondary shaking table activation at the temperature of 37-39 ℃, and inoculating the bacillus subtilis to the sterilized B culture medium after the bacillus subtilis reaches the standard after 24 hours, and performing shaking table expansion culture for 24 hours;
cellulase was purchased from Shandong Zhuo Hua Biotech, Inc.;
step 3, collecting bacterial sludge:
centrifuging the fermented bacillus coagulans liquid by using a centrifugal machine, and collecting bacterial sludge;
the method for collecting bacterial sludge of the bacillus subtilis is the same as that of the bacillus coagulans;
step 4, drying the bacterial sludge:
spray drying the bacillus coagulans bacterial mud collected in the step 3 by a spray drying treatment process to prepare the bacillus coagulans bacterial mud, which comprises the following specific steps: a. adding 15% of beta-cyclodextrin into the bacterial sludge, and uniformly mixing; b. the parameters of the spray dryer were set as follows: carrying out spray drying to prepare powder at the outlet temperature of 75-78 ℃, the inlet temperature of 125-128 ℃ and the feeding speed of 600 ml/h; c. the prepared fungus powder is stored in a sealed environment at-4 ℃;
the bacterial sludge spray drying method of the bacillus subtilis is the same as that of the bacillus coagulans;
step 5, preparing a finished product:
taking the bacterial powder prepared in the step 4, wherein 30 parts of bacillus coagulans and 20 parts of bacillus subtilis, 10 parts of cellulase and 140 parts of anhydrous glucose are uniformly mixed and bagged in an aseptic state, and the number of viable bacteria is 2 × 1010cfu/g, obtaining a composite microbial preparation with a degradation bioflocculant.
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