CN101058792A - Highly effective straw decomposition composite flora - Google Patents
Highly effective straw decomposition composite flora Download PDFInfo
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- CN101058792A CN101058792A CN 200710061529 CN200710061529A CN101058792A CN 101058792 A CN101058792 A CN 101058792A CN 200710061529 CN200710061529 CN 200710061529 CN 200710061529 A CN200710061529 A CN 200710061529A CN 101058792 A CN101058792 A CN 101058792A
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Abstract
The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.
Description
Technical field
The present invention relates to utilize the biotechnology of microbiological treatment agriculture waste stalk, be specially a kind of highly effective straw decomposition composite flora.
Background technology
Stalk comprises straw, wheat straw, corn stalk, beanstalk, sorghum stalks etc., is the tankage of food crop.In China rural area, most agricultural crop straws are directly burned, and have both caused serious environmental to pollute, and cause the waste of resource again.In recent years, people recognize gradually contains a large amount of Mierocrystalline celluloses, hemicellulose, xylogen and other organic substance in the agricultural crop straw, this part accounts for 60% of stalk dry matter weight, people are by utilizing microbial fermentation technology with straw degradative, the feed that can be used as ruminating animal on the one hand, can be used as fertilizer on the other hand, is the effective way of improving the soil, increase the soil organism.At present, the stalk fermentation technology that generally adopts mainly contains two kinds both at home and abroad, and a, aerobic fermentation promptly utilize mould, yeast, the cellulolytic aerobic bacteria of actinomycetes; B, anaerobically fermenting promptly utilize lactic acid fungus strain, bacillus cereus system to ferment.Facts have proved that there is following problem in prior art: 1, bacterial classification is more single, a little less than the capacity of decomposition, Mierocrystalline cellulose total degradation rate is low, is generally about 20-40%; 2, fermentation period is longer, generally needs 20-40 days.
Summary of the invention
The microbiobacterial agent kind that has now in the straw degradative process to be utilized is single in order to solve in the present invention, a little less than the capacity of decomposition, problem such as Mierocrystalline cellulose total degradation rate is low and fermentation period is long, a kind of highly effective straw decomposition composite flora is provided.
The present invention adopts following technical scheme to realize: a kind of highly effective straw decomposition composite flora, this flora is that the bacterial classification by following weight percent constitutes: Phanerochaete chrysosporium (Phanerochaete chrysosporium) 1.4-2.6 part, Lentinus Edodes fungus (Lentinus edodes) 1.4-2.6 part, trichoderma harziarum (Trichoderma Harzianum) 0.7-1.3 part, viride (Trichoderma viride) 0.7-1.3 part, healthy and free from worry wood mould (Trichoderma koningii) 0.7-1.3 part, aspergillus niger (Aspergillus niger) 0.7-1.3 part, subtilis (Bacillus subtilis) 0.73-1.24 part, feed genus bacillus (Paenibacillus pabuli) 0.73-1.24 part, bacillus megaterium (Bac.megatherium) 0.73-1.24 part, candiyeast (Candida shehatae) 0.14-0.26 part, distillery yeast (Alcohol Yeast) 0.14-0.26 part, food-yeast (Bread yeast) 0.07-0.13 part, aztobacter sp (Aztobacter SP) 0.14-0.26 part, thermophilic actinomycete (Thermoactinomycetaceae) 0.35-0.65 part, above-mentioned said bacterial classification all is commercially available, and is that those of ordinary skill in the art is easy to obtain.
During use, above-mentioned bacterial classification is mixed by above-mentioned weight ratio, then with pulverize after stalk mix and get final product.
In the above-mentioned flora, the main effect of Phanerochaete chrysosporium, Lentinus Edodes fungus is a lignin degrading; Trichoderma harziarum, viride, the mould main effect of healthy and free from worry wood are degraded celluloses; The main effect of aspergillus niger is a degradation of hemicellulose; The main effect of subtilis, feed genus bacillus, candiyeast, distillery yeast, food-yeast is degraded starch, protein, pectin substance, oxygen level, sugar degree in the balance feed liquid; The main effect of aztobacter sp is the nitrogen element of preserving in the raw material, removes stink; The main effect of thermophilic actinomycete is a rapid temperature-raising, assisted reaction.
The 1-5 ‰ that composite flora dosage of the present invention is a stalk weight.
Compared with prior art, the present invention is through a large amount of screenings, combination, multiple microorganisms such as fungi, bacterium and actinomycetes are grouped together, have the following advantages: 1, various organic substances in comprehensive degrading straw, the degradation rate height, can effectively decompose agricultural wastes, as: the reluctant material of general methods such as the xylogen in the materials such as stalk, wheat straw, leaf, weeds, Mierocrystalline cellulose (as: compost, feed ensiling, ammonification etc.), and the cycle is short, following about 15 days of suitable condition, degradation rate reaches more than 80%; 2, has wide application field, industry such as making, brewage as the processing of biomass energy production (stalk marsh gas), feed, fertilizer all can use, can effectively utilize by the agricultural wastes such as stalk that the script use is little, turn waste into wealth, increase peasant income, reduce production costs, the protection environment, the atmospheric pollution that minimizing causes because of crop straw burning, improvement agricultural, aquaculture ecotope; 3, the composite flora of making is a solid fungicide, but prolonged preservation, and bacterial content is big in the unit raw material, and is active high, in a word, has certain popularization value.
Embodiment
Embodiment 1: every part is 1 gram
A kind of highly effective straw decomposition composite flora, this flora are that the bacterial classification by following weight percent constitutes:
Phanerochaete chrysosporium 1.4 grams, Lentinus Edodes fungus 1.4 grams, trichoderma harziarum 0.7 gram, viride 0.7 gram, mould 0.7 gram of healthy and free from worry wood, aspergillus niger 0.7 gram, subtilis 0.73 gram, feed genus bacillus 0.73 gram, bacillus megaterium 0.73 gram, candiyeast 0.14 gram, distillery yeast 0.14 gram, food-yeast 0.07 gram, aztobacter sp 0.14 gram, thermophilic actinomycete 0.35 gram.After the bacterial classification of above-mentioned weight ratio mixed, get and ferment after stalk after wherein 1 gram is pulverized with 1000 grams mixes.
Embodiment 2: every part is 1 jin
A kind of highly effective straw decomposition composite flora, this flora are that the bacterial classification by following weight percent constitutes:
2.6 jin of Phanerochaete chrysosporiums, 2.6 jin of Lentinus Edodes fungus, 1.3 jin of trichoderma harziarums, 1.3 jin of virides, mould 1.3 jin of healthy and free from worry wood, 1.3 jin of aspergillus nigers, 1.2 jin of subtilises, 1.24 jin of feed genus bacillus, 1.24 jin of bacillus megateriums, 0.26 jin of candiyeast, 0.26 jin of distillery yeast, 0.13 jin of food-yeast, 0.26 jin of aztobacter sp, 0.65 jin of thermophilic actinomycete.After the bacterial classification of above-mentioned weight ratio mixed, get wherein 4 jin with 8000 jin of pulverizing after stalk ferment after mixing.
Embodiment 3: every part is 1 kilogram
A kind of highly effective straw decomposition composite flora, this flora are that the bacterial classification by following weight percent constitutes:
2.0 jin of Phanerochaete chrysosporiums, 2.0 kilograms of Lentinus Edodes fungus, 1.0 kilograms of trichoderma harziarums, 1.0 kilograms of virides, mould 0.8 kilogram of healthy and free from worry wood, 1.2 kilograms of aspergillus nigers, 0.85 kilogram of subtilis, 1.15 kilograms of feed genus bacillus, 0.95 kilogram of bacillus megaterium, 0.2 kilogram of candiyeast, 0.25 kilogram of distillery yeast, 0.1 kilogram of food-yeast, 0.19 kilogram of aztobacter sp, 0.45 kilogram of thermophilic actinomycete.After the bacterial classification of above-mentioned weight ratio mixed, get wherein 3 kilograms with 1000 kilograms of pulverizing after stalk ferment after mixing.
Embodiment 4: every part is 1 gram
A kind of highly effective straw decomposition composite flora, this flora are that the bacterial classification by following weight percent constitutes:
Phanerochaete chrysosporium 1.8 grams, Lentinus Edodes fungus 2.3 grams, trichoderma harziarum 0.85 gram, viride 1.0 grams, mould 1.15 grams of healthy and free from worry wood, aspergillus niger 0.95 gram, subtilis 0.90 gram, feed genus bacillus 1.24 grams, bacillus megaterium 0.73 gram, candiyeast 0.22 gram, distillery yeast 0.18 gram, food-yeast 0.09 gram, aztobacter sp 0.25 gram, thermophilic actinomycete 0.55 gram.After the bacterial classification of above-mentioned weight ratio mixed, get and ferment after stalk after wherein 5 grams are pulverized with 1000 grams mixes.
Embodiment 5: every part is 1 jin
A kind of highly effective straw decomposition composite flora, this flora are that the bacterial classification by following weight percent constitutes:
1.8 jin of Phanerochaete chrysosporiums, 1.4 jin of Lentinus Edodes fungus, 1.25 jin of trichoderma harziarums, 0.75 jin of viride, mould 0.90 jin of healthy and free from worry wood, 0.7 jin of aspergillus niger, 1.24 jin of subtilises, 1.15 jin of feed genus bacillus, 0.95 jin of bacillus megaterium, 0.19 jin of candiyeast, 0.20 jin of distillery yeast, 0.07 jin of food-yeast, 0.26 jin of aztobacter sp, 0.55 jin of thermophilic actinomycete.After the bacterial classification of above-mentioned weight ratio mixed, get wherein 4 jin with 1000 jin of pulverizing after stalk ferment after mixing.
Claims (2)
1, a kind of highly effective straw decomposition composite flora is characterized in that this flora is that bacterial classification by following weight percent constitutes:
Phanerochaete chrysosporium (Phanerochaete chrysosporium) 1.4-2.6 part, Lentinus Edodes fungus (Lentinus edodes) 1.4-2.6 part, trichoderma harziarum (Trichoderma Harzianum) 0.7-1.3 part, viride (Trichoderma viride) 0.7-1.3 part, healthy and free from worry wood mould (Trichoderma koningii) 0.7-1.3 part, aspergillus niger (Aspergillus niger) 0.7-1.3 part, subtilis (Bacillus subtilis) 0.73-1.24 part, feed genus bacillus (Paenibacillus pabuli) 0.73-1.24 part, bacillus megaterium (Bac.megatherium) 0.73-1.24 part, candiyeast (Candida shehatae) 0.14-0.26 part, distillery yeast (Alcohol Yeast) 0.14-0.26 part, food-yeast (Bread yeast) 0.07-0.13 part, aztobacter sp (AztobacterSP) 0.14-0.26 part, thermophilic actinomycete (Thermoact inomycetaceae) 0.35-0.65 part.
2, a kind of highly effective straw decomposition composite flora according to claim 1 is characterized in that: the 1-5 ‰ that this composite flora dosage is a stalk weight.
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