CN102357500A - Method for treating crop straw - Google Patents
Method for treating crop straw Download PDFInfo
- Publication number
- CN102357500A CN102357500A CN2011101147207A CN201110114720A CN102357500A CN 102357500 A CN102357500 A CN 102357500A CN 2011101147207 A CN2011101147207 A CN 2011101147207A CN 201110114720 A CN201110114720 A CN 201110114720A CN 102357500 A CN102357500 A CN 102357500A
- Authority
- CN
- China
- Prior art keywords
- microbial inoculum
- stalk
- straw
- fermentation
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000010902 straw Substances 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 114
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 103
- 241000894006 Bacteria Species 0.000 claims abstract description 101
- 238000000855 fermentation Methods 0.000 claims abstract description 57
- 230000004151 fermentation Effects 0.000 claims abstract description 57
- 238000003756 stirring Methods 0.000 claims abstract description 33
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 241000194108 Bacillus licheniformis Species 0.000 claims description 32
- 244000063299 Bacillus subtilis Species 0.000 claims description 32
- 241000223261 Trichoderma viride Species 0.000 claims description 32
- 239000002131 composite material Substances 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 238000002360 preparation method Methods 0.000 claims description 24
- 238000010298 pulverizing process Methods 0.000 claims description 24
- 230000001580 bacterial effect Effects 0.000 claims description 22
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 17
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 17
- 238000011081 inoculation Methods 0.000 claims description 17
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 16
- 241000123346 Chrysosporium Species 0.000 claims description 16
- 241000235342 Saccharomycetes Species 0.000 claims description 16
- 241000222355 Trametes versicolor Species 0.000 claims description 16
- 241000223259 Trichoderma Species 0.000 claims description 16
- 241000378866 Trichoderma koningii Species 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 16
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 6
- 244000105624 Arachis hypogaea Species 0.000 claims description 6
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 6
- 235000018262 Arachis monticola Nutrition 0.000 claims description 6
- 235000020232 peanut Nutrition 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 244000000231 Sesamum indicum Species 0.000 claims description 4
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 240000006394 Sorghum bicolor Species 0.000 claims description 3
- 235000015505 Sorghum bicolor subsp. bicolor Nutrition 0.000 claims description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 abstract description 24
- 238000006731 degradation reaction Methods 0.000 abstract description 24
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000005507 spraying Methods 0.000 abstract 1
- 229920002488 Hemicellulose Polymers 0.000 description 9
- 229920005610 lignin Polymers 0.000 description 9
- 229920002678 cellulose Polymers 0.000 description 8
- 239000001913 cellulose Substances 0.000 description 8
- 238000012856 packing Methods 0.000 description 7
- 239000002361 compost Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 235000005473 carotenes Nutrition 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000004176 ammonification Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- -1 carotene carotene Chemical class 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- 239000008104 plant cellulose Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000004016 soil organic matter Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a method for treating crop straw. The method for treating crop straw is characterized by comprising the following steps: 1) preparing a mixed microbial inoculum which comprises A microbial inoculum and B microbial inoculum; 2) crushing the raw material straw; 3) preparing fermentation bacteria liquid A; 4) inoculating the bacteria liquid A for fermentation, that is, uniformly spraying the bacteria liquid A on crushed straw, piling crushed straw into a stockpile, wherein, the mass ratio of crushed straw to the bacteria liquid A is 1:1-1.5, maintaining the temperature of the stockpile to be in a range of 25 to 35 DEG C, and allowing fermentation to last for 4 to 7 days so as to obtain straw which has inoculated the bacteria liquid A and fermented for 4 to 7 days; 3) preparing fermentation bacteria liquid B; 6) inoculating the bacteria liquid B for fermentation, that is, stirring the straw which has inoculated the bacteria liquid A and fermented for 4 to 7 days, and uniformly mixing the straw with the bacteria liquid B according to a mass ratio of 20-30:1, maintaining the temperature of the stockpile to be in a range of 25 to 35 DEG C, and continuing fermentation for 5 to 8 days so as to finish the whole process of fermentation. The method has the characteristics of low production cost and high degradation efficiency.
Description
Technical field
The present invention relates to a kind of processing method of agricultural crop straw, be specifically related to a kind of method of utilizing microorganism efficient degradation agricultural crop straw.
Background technology
China is a large agricultural country, according to rough Statistics, produces more than 700,000,000 ton of agricultural crop straw every year approximately, and 89% be not utilized as yet or be not used appropriately (like direct burning) wherein arranged.The agricultural fiber accessory substance less than 2% that the present whole world is exploited, China has the agriculture and forestry organic waste material more than 50% to be burnt in vain in the field approximately.Not only cause direct economic loss to reach several hundred million units because of agriculture and forestry organic waste material burns every year; And had a strong impact on the safety of highway, aviation owing to a large amount of pernicious gases of burning the thick smoke produce and discharging; Polluted environment, weather, ecology etc. has also been caused seriously influence.Along with the develop rapidly of global economy, the reserves of oil, coal reduce just with surprising rapidity on the earth, the severe problem that energy crisis has become world's most countries to be faced simultaneously.In addition owing to irrational extraction of resources and utilization to resource; The environment that the mankind depend on for existence constantly worsens; The strategy of sustainable development to the research and development of renewable resource utilization is progressively launched in countries in the world, and the development and use of plant cellulose resource have extremely profound significance to solving grain and shortage of resources and problem of environmental pollution.
At present, the main outlet of stalk has straw-returning; The feed utilization; Gasifying stalk, curing and charing; Make to produce the culture base-material of edible mushroom; Commercial Application etc.Straw-returning comprises on the spot also the field, makes compost and compost etc. fast.Wherein straw directly returning to field can increase soil organic matter content, fertilizing soil, and is significant to the sustainable development of agricultural, but also has a lot of problems still unresolved.At first, the C/N of stalk not only bad for soil microbial degradation, but also possibly cause the crop nitrogen stress and undergrowth than high; Secondly, stalk is possibility part of propagation disease also, causes crop failure etc.Make compost fast and then have the degradation problem of lignocellulosic with compost.The feed technology mainly comprises ensiling ammonification, fermentation method and manufacture order cell protein.The agricultural crop straw that can be used as feed in China has multiple, but has common nutritional characteristic: protein, soluble-carbohydrate, mineral matter and carotene carotene content are low, and crude fiber content is high, so digestibility is low, and palatability is poor.Improve palatability, improve digestibility, key is the degraded of lignocellulosic.Commercial Application comprises papermaking, and starch, alcohol, acetic acid etc. are produced in biodegradation, is prone to cause serious environmental to pollute, and has the degradation problem of lignocellulosic equally.
Because lignocellulosic self molecular weight is big, lignin, hemicellulose and cellulose tangle each other, complex structure, and stable in properties so the physics, the chemical degradation method that generally use at present all can not effectively be degraded, and is brought cost and environmental problem.Biological method has environment friendly and pollution-free, and cost is low, and characteristics such as service condition gentleness are having great potential aspect fast effective lignocellulose degradation class material.Patent of invention (CN101948753A) discloses " a kind of composite bacteria agent capable of novel degraded agricultural crop straw ", when composite bacteria agent capable is used for straw degradative, need in about 30 ℃, ferment 30 days; The degradation rate of lignin is low to be merely 27.8%; And owing to lack the bacterial strain of degradation of hemicellulose, therefore, this composite bacteria agent capable exists each bacterial strain synergy less when degrading straw; Fermentation time is long, the low problem of the whole degradation rate of lignocellulosic.Patent of invention (CN101058792A) discloses " a kind of highly effective straw decomposition composite flora "; Flora is grouped together by 14 kinds of bacterial classifications such as comprising fungi, bacterium and actinomyces; Yet in the described flora of this invention healthy and free from worry wood mould with flora in other bacterial strain such as bacterium such as Phanerochaete chrysosporium, withered grass bud robe bacillus and Candida strong nutrient competition and antagonism are arranged; Simply combine and to cause the flora decrease in efficiency; Consume each other, cause great waste, increase production cost.
Summary of the invention
The object of the present invention is to provide a kind of method of handling agricultural crop straw, this method production cost is low, degradation rate is high.
For realizing above-mentioned purpose, the technical scheme that the present invention adopted is: a kind of method of handling agricultural crop straw is characterized in that it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is formed (microbial inoculum of two groups of independent packing constitutes) by A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 28%～38%, variable color bolt bacterium (Trametes versicolor) 5%～10%, flat mushroom bacterium (Pleurorus ostreatus) 6%～12%, this saccharomycete of Crewe (Canadian krusei) 7～14%, Bacillus subtillis (Bacillus Subtilis) 6%～11% and bacillus licheniformis (Bacillus Licheniformis) 15～25%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 30%～40% and koning trichoderma (Trichoderma koningii) 60%～70%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: agricultural crop straw is put into pulverizer, be ground into the segment of 1cm～3cm, the stalk after obtaining pulverizing;
3) preparation of zymocyte liquid A: the taking-up of A microbial inoculum is mixed stirring (the A microbial inoculum is evenly spread in the water) with water by mass ratio 1: 100～200, obtain bacterium liquid; In bacterium liquid, add carbonic hydroammonium then, stir (carbonic hydroammonium is fully dissolved), obtain zymocyte liquid A; Wherein, the addition of carbonic hydroammonium is 2～3% of a bacterium liquid quality;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after the pulverizing, pile stockpile, the mass ratio of stalk after the pulverizing and zymocyte liquid A is 1: 1～1.5; The temperature that keeps stockpile is (if when winter, temperature was low, covering stockpile) between 25～35 ℃ with film, continuing fermentation 4～7 days has been inoculated the stalk that bacterium liquid A has fermented 4～7 days;
5) preparation of zymocyte liquid B: the taking-up of B microbial inoculum is mixed stirring (the B microbial inoculum is evenly spread in the water) with water by mass ratio 1: 30～40, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 4～7 days fully stirs and with zymocyte liquid B by mass ratio 19～30: 1 mixing; Keep the stockpile temperature between 25～35 ℃, continuing fermentation is after 5～8 days, and whole fermentation process finishes.
Press such scheme; Described agricultural crop straw is any one or any mixing that (contains any two kinds) more than two kinds in the stalks such as rape stalk, cotton stalk, sesame stalk, peanut stalk, rice straw, wheat straw stalk, maize straw, beanstalk stalk, broomcorn straw, and any is any proportioning when (containing any two kinds) more than two kinds and mixing.
The invention has the beneficial effects as follows:
1, related bacterial strain, medicament and equipment is easy above market among the present invention, and production cost is low.
2, method of the present invention is easy and simple to handle, and the condition temperature is closed, and control realizes no difficulty easily.
3, each bacterial strain all carried out compatible experiment each other in the composite microbial inoculum A microbial inoculum according to the invention, did not have antagonism and obvious nutrient competition situation, therefore, can work in coordination with each other, and fermentation time is short, and degradation efficiency is high; The B microbial inoculum is like this too.
4, composite microbial inoculum according to the invention includes degradation bacteria such as cellulose, hemicellulose, lignin, protein and pectin, and is wide in variety, collocation rationally, various organic substances in the degrading straw comprehensively.
5, method of the present invention after a period of time, inoculates the B microbial inoculum through choosing inoculation A bacteria fermentation, can give full play to the effect of each microbial inoculum, can effectively avoid and alleviate the nutrient competition effect that exists between A and the B microbial inoculum again, so whole efficiency is higher.
The specific embodiment
Preferred version of the present invention is described below; It is included within the scope of the present invention's protection, but does not limit the present invention.
Embodiment 1:
A kind of method of handling agricultural crop straw, it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is formed (microbial inoculum of two groups of independent packing constitutes) by A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 28%, variable color bolt bacterium (Trametes versicolor) 10%, flat mushroom bacterium (Pleurorus ostreatus) 12%, this saccharomycete of Crewe (Canadian krusei) 14%, Bacillus subtillis (Bacillus Subtilis) 11% and bacillus licheniformis (Bacillus Licheniformis) 25%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 30% and koning trichoderma (Trichoderma koningii) 70%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: the 100kg rape stalk is put into pulverizer, be ground into the segment of 2cm, the stalk after obtaining pulverizing;
3) preparation of zymocyte liquid A: the A microbial inoculum 0.5kg that takes by weighing in the composite microbial inoculum mixes stirring (the A microbial inoculum is evenly spread in the water) with 100kg water, obtain bacterium liquid; In bacterium liquid, add 2.5kg carbonic hydroammonium (addition of carbonic hydroammonium be bacterium liquid quality 2.49%) then; Stir (carbonic hydroammonium is fully dissolved), obtain zymocyte liquid A;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after 100kg pulverizes, pile stockpile; The temperature that keeps stockpile is (if when winter, temperature was low, covering stockpile) about 30 ℃ with film, continuing fermentation 6 days has been inoculated the stalk that bacterium liquid A has fermented 6 days;
5) preparation of zymocyte liquid B: the B microbial inoculum 0.25kg that gets in the composite microbial inoculum mixes stirring (the B microbial inoculum is evenly spread in the water) with 10kg water, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 6 days fully stirs and with zymocyte liquid B by mass ratio 20～30: 1 mixing; Keep the stockpile temperature about 30 ℃, continuing fermentation is after 7 days, and whole fermentation process finishes.
The degradation rate that records cellulose, hemicellulose and lignin in the rape stalk after the fermentation is respectively 46%, 38% and 42%, explains that degradation rate of the present invention is high.
Embodiment 2:
A kind of method of handling agricultural crop straw, it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is formed (microbial inoculum of two groups of independent packing constitutes) by A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 38%, variable color bolt bacterium (Trametes versicolor) 10%, flat mushroom bacterium (Pleurorus ostreatus) 12%, this saccharomycete of Crewe (Canadian krusei) 14%, Bacillus subtillis (Bacillus Subtilis) 11% and bacillus licheniformis (Bacillus Licheniformis) 15%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 40% and koning trichoderma (Trichoderma koningii) 60%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: the 100kg rice straw is put into pulverizer, be ground into the segment of 3cm, the stalk after obtaining pulverizing;
3) preparation of zymocyte liquid A: the A microbial inoculum 0.6kg that takes by weighing in the composite microbial inoculum mixes stirring (the A microbial inoculum is evenly spread in the water) with 120kg water, obtain bacterium liquid; In bacterium liquid, add the carbonic hydroammonium of 2.42kg then, stir (carbonic hydroammonium is fully dissolved), obtain zymocyte liquid A;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after 100kg pulverizes, pile stockpile; The temperature that keeps stockpile is (if when winter, temperature was low, covering stockpile) about 28 ℃ with film, continuing fermentation 5 days has been inoculated the stalk that bacterium liquid A has fermented 5 days;
5) preparation of zymocyte liquid B: the B microbial inoculum 0.3kg that gets in the composite microbial inoculum mixes stirring (the B microbial inoculum is evenly spread in the water) with 9kg water, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 5 days fully stirs and with zymocyte liquid B mixing; Keep the stockpile temperature about 28 ℃, continuing fermentation is after 6 days, and whole fermentation process finishes.
The degradation rate that records cellulose, hemicellulose and lignin in the rice straw after the fermentation is respectively 45%, 39% and 43%, explains that degradation rate of the present invention is high.
Embodiment 3:
A kind of method of handling agricultural crop straw, it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is formed (microbial inoculum of two groups of independent packing constitutes) by A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 38%, variable color bolt bacterium (Trametes versicolor) 5%, flat mushroom bacterium (Pleurorus ostreatus) 7%, this saccharomycete of Crewe (Canadian krusei) 14%, Bacillus subtillis (Bacillus Subtilis) 11% and bacillus licheniformis (Bacillus Licheniformis) 25%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 35% and koning trichoderma (Trichoderma koningii) 65%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: 100kg sesame stalk is put into pulverizer, be ground into the segment of 3cm, the stalk after obtaining pulverizing;
3) preparation of zymocyte liquid A: the A microbial inoculum 0.6kg that takes by weighing in the composite microbial inoculum mixes stirring (the A microbial inoculum is evenly spread in the water) with 100kg water, obtain bacterium liquid; In bacterium liquid, add the carbonic hydroammonium of 2.6kg then, stir (carbonic hydroammonium is fully dissolved), obtain zymocyte liquid A;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after 100kg pulverizes, pile stockpile; The temperature that keeps stockpile is (if when winter, temperature was low, covering stockpile) about 30 ℃ with film, continuing fermentation 6 days has been inoculated the stalk that bacterium liquid A has fermented 6 days;
5) preparation of zymocyte liquid B: the B microbial inoculum 0.3kg that gets in the composite microbial inoculum mixes stirring (the B microbial inoculum is evenly spread in the water) with 10kg water, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 6 days fully stirs and with zymocyte liquid B mixing; Keep the stockpile temperature about 30 ℃, continuing fermentation is after 6 days, and whole fermentation process finishes.
The degradation rate that records cellulose, hemicellulose and lignin in the sesame stalk after the fermentation is respectively 42%, 37% and 40%, explains that degradation rate of the present invention is high.
Embodiment 4:
A kind of method of handling agricultural crop straw is characterized in that it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is formed (microbial inoculum of two groups of independent packing constitutes) by A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 38%, variable color bolt bacterium (Trametes versicolor) 6%, flat mushroom bacterium (Pleurorus ostreatus) 6%, this saccharomycete of Crewe (Canadian krusei) 14%, Bacillus subtillis (Bacillus Subtilis) 11% and bacillus licheniformis (Bacillus Licheniformis) 25%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 32% and koning trichoderma (Trichoderma koningii) 68%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: 100kg peanut stalk is put into pulverizer, be ground into the segment of 1cm, the stalk after obtaining pulverizing;
3) preparation of zymocyte liquid A: the A microbial inoculum 0.6kg that takes by weighing in the composite microbial inoculum mixes stirring (the A microbial inoculum is evenly spread in the water) with 120kg water, obtain bacterium liquid; In bacterium liquid, add the carbonic hydroammonium of 2.7kg then, stir (carbonic hydroammonium is fully dissolved), obtain zymocyte liquid A;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after 100kg pulverizes, pile stockpile; The temperature that keeps stockpile is (if when winter, temperature was low, covering stockpile) between 25～35 ℃ with film, continuing fermentation 7 days has been inoculated the stalk that bacterium liquid A has fermented 7 days;
5) preparation of zymocyte liquid B: the B microbial inoculum 0.25kg that gets in the composite microbial inoculum mixes stirring (the B microbial inoculum is evenly spread in the water) with 10kg water, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 7 days fully stirs and with zymocyte liquid B mixing; Keep the stockpile temperature about 31 ℃, continuing fermentation is after 8 days, and whole fermentation process finishes.
The degradation rate that records cellulose, hemicellulose and lignin in the peanut stalk after the fermentation is respectively 49%, 41% and 45%, explains that degradation rate of the present invention is high.
Embodiment 5:
A kind of method of handling agricultural crop straw, it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is formed (microbial inoculum of two groups of independent packing constitutes) by A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 38%, variable color bolt bacterium (Trametes versicolor) 10%, flat mushroom bacterium (Pleurorus ostreatus) 12%, this saccharomycete of Crewe (Canadian krusei) 7%, Bacillus subtillis (Bacillus Subtilis) 11% and bacillus licheniformis (Bacillus Licheniformis) 22%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 31% and koning trichoderma (Trichoderma koningii) 69%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: agricultural crop straw is put into pulverizer, be ground into the segment of 1cm, the stalk after obtaining pulverizing; Described agricultural crop straw is cotton stalk and wheat straw stalk, and the quality of cotton stalk and wheat straw stalk respectively accounts for 50%;
3) preparation of zymocyte liquid A: the taking-up of A microbial inoculum is mixed stirring (the A microbial inoculum is evenly spread in the water) with water by mass ratio at 1: 100, obtain bacterium liquid; In bacterium liquid, add carbonic hydroammonium then, stir (carbonic hydroammonium is fully dissolved), obtain zymocyte liquid A; Wherein, the addition of carbonic hydroammonium is 2% of a bacterium liquid quality;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after the pulverizing, pile stockpile, the mass ratio of stalk after the pulverizing and zymocyte liquid A is 1: 1; The temperature that keeps stockpile is 25 ℃ (if when winter, temperature was low, covering stockpile) with film, continuing fermentation 4 days has been inoculated the stalk that bacterium liquid A has fermented 4 days;
5) preparation of zymocyte liquid B: the taking-up of B microbial inoculum is mixed stirring (the B microbial inoculum is evenly spread in the water) with water by mass ratio at 1: 30, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 4 days fully stirs and with zymocyte liquid B by 19: 1 mixings of mass ratio; Keep the stockpile temperature at 25 ℃, continuing fermentation is after 5 days, and whole fermentation process finishes.
The degradation rate that records cellulose, hemicellulose and lignin in the peanut stalk after the fermentation is respectively 42%, 39% and 41%, explains that degradation rate of the present invention is high.
Embodiment 6:
A kind of method of handling agricultural crop straw, it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is formed (microbial inoculum of two groups of independent packing constitutes) by A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 38%, variable color bolt bacterium (Trametes versicolor) 10%, flat mushroom bacterium (Pleurorus ostreatus) 12%, this saccharomycete of Crewe (Canadian krusei) 14%, Bacillus subtillis (Bacillus Subtilis) 6% and bacillus licheniformis (Bacillus Licheniformis) 20%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 39% and koning trichoderma (Trichoderma koningii) 61%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: agricultural crop straw is put into pulverizer, be ground into the segment of 3cm, the stalk after obtaining pulverizing; Described agricultural crop straw is beanstalk stalk and broomcorn straw, and the quality of beanstalk stalk and jowar stalk respectively accounts for 50%;
3) preparation of zymocyte liquid A: the taking-up of A microbial inoculum is mixed stirring (the A microbial inoculum is evenly spread in the water) with water by mass ratio at 1: 200, obtain bacterium liquid; In bacterium liquid, add carbonic hydroammonium then, stir (carbonic hydroammonium is fully dissolved), obtain zymocyte liquid A; Wherein, the addition of carbonic hydroammonium is 3% of a bacterium liquid quality;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after the pulverizing, pile stockpile, the mass ratio of stalk after the pulverizing and zymocyte liquid A is 1: 1.5; The temperature that keeps stockpile is (if when winter, temperature was low, covering stockpile) between 35 ℃ with film, continuing fermentation 7 days has been inoculated the stalk that bacterium liquid A has fermented 7 days;
5) preparation of zymocyte liquid B: the taking-up of B microbial inoculum is mixed stirring (the B microbial inoculum is evenly spread in the water) with water by mass ratio at 1: 40, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 7 days fully stirs and with zymocyte liquid B by 30: 1 mixings of mass ratio; Keep the stockpile temperature between 35 ℃, continuing fermentation is after 8 days, and whole fermentation process finishes.
The degradation rate that records cellulose, hemicellulose and lignin in the peanut stalk after the fermentation is respectively 50%, 43% and 47%, explains that degradation rate of the present invention is high.
Each raw material that the present invention is cited, and the bound of each raw material of the present invention, interval value, and the bound of technological parameter (like temperature, time etc.), interval value can both realize the present invention, do not enumerate embodiment one by one at this.
Claims (2)
1. a method of handling agricultural crop straw is characterized in that it comprises the steps:
1) preparation of composite microbial inoculum: this composite microbial inoculum is made up of A microbial inoculum and B microbial inoculum;
1. by the shared mass percent of each bacterial classification be: Phanerochaete chrysosporium (Phaerochaete chrysosporium) 28%～38%, variable color bolt bacterium (Trametes versicolor) 5%～10%, flat mushroom bacterium (Pleurorus ostreatus) 6%～12%, this saccharomycete of Crewe (Canadian krusei) 7～14%, Bacillus subtillis (Bacillus Subtilis) 6%～11% and bacillus licheniformis (Bacillus Licheniformis) 15～25%; Choose Phanerochaete chrysosporium (Phaerochaete chrysosporium), variable color bolt bacterium (Trametes versicolor), flat mushroom bacterium (Pleurorus ostreatus), this saccharomycete of Crewe (Canadian krusei), Bacillus subtillis (Bacillus Subtilis) and bacillus licheniformis (Bacillus Licheniformis); Mix, obtain the A microbial inoculum;
2. by the shared mass percent of each bacterial classification be: Trichoderma viride (Trichoderma Viride) 30%～40% and koning trichoderma (Trichoderma koningii) 60%～70%; Choose Trichoderma viride (Trichoderma Viride) and koning trichoderma (Trichoderma koningii); Mix, obtain the B microbial inoculum;
2) pulverizing of stalk: agricultural crop straw is put into pulverizer, be ground into the segment of 1cm～3cm, the stalk after obtaining pulverizing;
3) preparation of zymocyte liquid A: the taking-up of A microbial inoculum is mixed stirring with water by mass ratio 1: 100～200, obtain bacterium liquid; In bacterium liquid, add carbonic hydroammonium then, stir, obtain zymocyte liquid A; Wherein, the addition of carbonic hydroammonium is 2～3% of a bacterium liquid quality;
4) inoculation bacterium liquid A fermentation: zymocyte liquid A is evenly sent forth on the stalk after the pulverizing, pile stockpile, the mass ratio of stalk after the pulverizing and zymocyte liquid A is 1: 1～1.5; The temperature that keeps stockpile is between 25～35 ℃, and continuing fermentation 4～7 days has been inoculated the stalk that bacterium liquid A has fermented 4～7 days;
5) preparation of zymocyte liquid B: the taking-up of B microbial inoculum is mixed stirring with water by mass ratio 1: 30～40, obtain zymocyte liquid B;
6) inoculation bacterium liquid B fermentation: will inoculate that stalk that bacterium liquid A fermented 4～7 days stirs and with zymocyte liquid B by mass ratio 19～30: 1 mixing; Keep the stockpile temperature between 25～35 ℃, continuing fermentation is after 5～8 days, and whole fermentation process finishes.
2. a kind of method of handling agricultural crop straw according to claim 1; It is characterized in that: described agricultural crop straw is any one or any mixing more than two kinds in rape stalk, cotton stalk, sesame stalk, peanut stalk, rice straw, wheat straw stalk, maize straw, beanstalk stalk, the broomcorn straw, and any is any proportioning when mixing more than two kinds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110114720.7A CN102357500B (en) | 2011-05-05 | 2011-05-05 | Method for treating crop straw |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110114720.7A CN102357500B (en) | 2011-05-05 | 2011-05-05 | Method for treating crop straw |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102357500A true CN102357500A (en) | 2012-02-22 |
| CN102357500B CN102357500B (en) | 2014-03-12 |
Family
ID=45582912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110114720.7A Expired - Fee Related CN102357500B (en) | 2011-05-05 | 2011-05-05 | Method for treating crop straw |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102357500B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156059A (en) * | 2013-03-20 | 2013-06-19 | 黑龙江省乳家农业科技开发有限责任公司 | Preparation method of high-protein and low-fiber biological nutrition maize straw forage grass |
| CN103230917A (en) * | 2013-03-14 | 2013-08-07 | 东北林业大学 | Forest floor combustible biodegradation agent and use thereof |
| CN108220181A (en) * | 2016-12-14 | 2018-06-29 | 康盛时代（北京）生物技术有限公司 | A kind of composite flora includes its composite bacteria agent and preparation method thereof and purposes |
| CN108330087A (en) * | 2018-02-05 | 2018-07-27 | 信阳农林学院 | A kind of solid fermentation agent for fermenting peanut stalk |
| CN110353087A (en) * | 2019-07-11 | 2019-10-22 | 南京农业大学 | The preparation method and fermented feed of rape stalk fermented feed |
| CN112239378A (en) * | 2020-10-23 | 2021-01-19 | 张洪江 | Method for efficiently producing biochemical fulvic acid fertilizer and high-quality natural fibers by using rice straws |
| CN112609491A (en) * | 2020-11-16 | 2021-04-06 | 黑龙江进化树生物科技有限公司 | Biological pollution-free pulping method of rice straw |
| WO2021062887A1 (en) * | 2019-09-30 | 2021-04-08 | 广东普洛宇飞生物科技有限公司 | Straw biodegradable composite bacterial agent and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101054552A (en) * | 2007-04-05 | 2007-10-17 | 清华大学 | Microorganism composite bacterium agent and preparation method and application thereof |
| CN101058792A (en) * | 2007-02-13 | 2007-10-24 | 韩农 | Highly effective straw decomposition composite flora |
| US20080320615A1 (en) * | 2001-02-02 | 2008-12-25 | Johnson Thomas D | Plant seed assemblies comprising bacterial/fungal antagonists |
| CN101401624A (en) * | 2008-11-12 | 2009-04-08 | 孟繁宇 | Corn stalk nano-dietary fiber and method of preparing the same |
| CN101891509A (en) * | 2010-03-22 | 2010-11-24 | 中国农业科学院油料作物研究所 | Compound microbial fertilizer and preparation method thereof |
-
2011
- 2011-05-05 CN CN201110114720.7A patent/CN102357500B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080320615A1 (en) * | 2001-02-02 | 2008-12-25 | Johnson Thomas D | Plant seed assemblies comprising bacterial/fungal antagonists |
| CN101058792A (en) * | 2007-02-13 | 2007-10-24 | 韩农 | Highly effective straw decomposition composite flora |
| CN101054552A (en) * | 2007-04-05 | 2007-10-17 | 清华大学 | Microorganism composite bacterium agent and preparation method and application thereof |
| CN101401624A (en) * | 2008-11-12 | 2009-04-08 | 孟繁宇 | Corn stalk nano-dietary fiber and method of preparing the same |
| CN101891509A (en) * | 2010-03-22 | 2010-11-24 | 中国农业科学院油料作物研究所 | Compound microbial fertilizer and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘睿，等: "秸秆预处理技术存在的问题与发展前景", 《环境科学与技术》 * |
| 刘睿: "油料秸秆多菌共发酵降解体系的建立及初步应用", 《中国优秀硕士学位论文全文数据库，农业科技辑》 * |
| 黄茜: "秸秆二步混合发酵降解体系的组建及优化", 《中国优秀硕士学位论文全文数据库，农业科技辑》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103230917A (en) * | 2013-03-14 | 2013-08-07 | 东北林业大学 | Forest floor combustible biodegradation agent and use thereof |
| CN103156059A (en) * | 2013-03-20 | 2013-06-19 | 黑龙江省乳家农业科技开发有限责任公司 | Preparation method of high-protein and low-fiber biological nutrition maize straw forage grass |
| CN103156059B (en) * | 2013-03-20 | 2014-04-16 | 黑龙江省乳家农业科技开发有限责任公司 | Preparation method of high-protein and low-fiber biological nutrition maize straw forage grass |
| CN108220181A (en) * | 2016-12-14 | 2018-06-29 | 康盛时代（北京）生物技术有限公司 | A kind of composite flora includes its composite bacteria agent and preparation method thereof and purposes |
| CN108330087A (en) * | 2018-02-05 | 2018-07-27 | 信阳农林学院 | A kind of solid fermentation agent for fermenting peanut stalk |
| CN108330087B (en) * | 2018-02-05 | 2021-07-20 | 信阳农林学院 | Solid leaven for fermenting peanut straw |
| CN110353087A (en) * | 2019-07-11 | 2019-10-22 | 南京农业大学 | The preparation method and fermented feed of rape stalk fermented feed |
| WO2021062887A1 (en) * | 2019-09-30 | 2021-04-08 | 广东普洛宇飞生物科技有限公司 | Straw biodegradable composite bacterial agent and application thereof |
| CN112239378A (en) * | 2020-10-23 | 2021-01-19 | 张洪江 | Method for efficiently producing biochemical fulvic acid fertilizer and high-quality natural fibers by using rice straws |
| CN112239378B (en) * | 2020-10-23 | 2021-10-26 | 张洪江 | Method for efficiently producing biochemical fulvic acid fertilizer and high-quality natural fibers by using rice straws |
| CN112609491A (en) * | 2020-11-16 | 2021-04-06 | 黑龙江进化树生物科技有限公司 | Biological pollution-free pulping method of rice straw |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102357500B (en) | 2014-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102357500B (en) | Method for treating crop straw | |
| CN101225004B (en) | Method for producing soil biological fertilizer by employing compound starter | |
| CN104926541B (en) | A kind of production technology of joint wheat bran stalk vinasse seaweed mixed fungus fermentation efficient biologic-organic fertilizer | |
| CN102503611B (en) | Organic fertilizer or bio-mixed organic fertilizer special for rice and preparing method thereof | |
| CN102249756B (en) | Biological organic fertilizer using orange peel residue as main raw material and preparation method thereof | |
| CN102517217B (en) | Environment-friendly straw fermenting agent | |
| CN103896653B (en) | A kind of preparation method of fertilizer | |
| CN103642726B (en) | A kind of maize straw organism of fermentation microbial inoculum and preparation method thereof and fermentation process | |
| CN106083264A (en) | A kind of recycling wheat stalk prepares the new technology of biological organic fertilizer | |
| CN105733975A (en) | Multifunctional high efficient organic material decomposition agent and preparation method thereof | |
| CN106631248A (en) | Production process for bioorganic fertilizer with high bacteria content | |
| CN102584371A (en) | Method for making biological organic fertilizer by using rice straws as main raw material | |
| CN102174400A (en) | Microbial degrading agent for decomposing cassava stalks and preparation method | |
| CN105746028A (en) | Straw decomposing field-returning method capable of effectively reducing environmental pollution | |
| CN105820018A (en) | Biological soil conditioner | |
| CN103614315B (en) | A kind of bacterium chaff pyrolytic decomposition composite fungus agent and preparation method thereof | |
| CN101823916A (en) | Preparation of total-nutrient efficient biological compound fertilizer and special microbial strain | |
| CN104357362A (en) | Complex microbial agent for fast decaying straws to produce organic fertilizer as well as preparation method and application | |
| CN107522541A (en) | Biological organic fertilizer produced using chicken manure and furfural dregs and preparation method thereof | |
| CN104099281A (en) | Environment-friendly compound microbial agent | |
| CN104561121A (en) | Method for preparing marsh gas by anaerobic fermentation of agricultural straws | |
| CN101899395A (en) | Screening and construction method for composite microbial system for rapidly degrading rice straw and composite microbial system | |
| CN102199051A (en) | Trichoderma microbial fertilizer production by reutilizing livestock and poultry manure | |
| CN104099282B (en) | Environment-friendly stalk degradation agents | |
| CN102887750A (en) | Method for preparing organic fertilizer by fermenting rapeseed cakes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140312 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |