CN1544610A - Microorganism compositions for degrading straw, preparation thereof and method for degradation of straw - Google Patents

Microorganism compositions for degrading straw, preparation thereof and method for degradation of straw Download PDF

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Publication number
CN1544610A
CN1544610A CNA2003101070709A CN200310107070A CN1544610A CN 1544610 A CN1544610 A CN 1544610A CN A2003101070709 A CNA2003101070709 A CN A2003101070709A CN 200310107070 A CN200310107070 A CN 200310107070A CN 1544610 A CN1544610 A CN 1544610A
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parts
straw
degrading
spawn
microbial composite
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陈秀为
赵华
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TIANJIN CITY AGRICULTURAL BIO-TECH RESEARCH CENTER
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TIANJIN CITY AGRICULTURAL BIO-TECH RESEARCH CENTER
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Abstract

The invention discloses a microbe combination of degraded stalks and its preparing method as well as a method of degrading the stalks, where the microbe combination includes: liquid bacterial strain, and solid bacterial strain, the liquid bacterial strain is made by culturing plant lactobacillus, Streptococcus faecali, and Candida utilis in the culture medium and the solid bacterial strain is made by culturing Phanerochaete chrysosporium and Pleurotus ostreatus; the method of degrading the stalks by the combination: mixing the liquid bacterial strain with the stalks raw material in weight ratio of 1 to 15000-25000, adding in 0.9% NaCl water solution to make the water ratio reach 65-70%, sealing and fermenting in a sealed container as 25-30deg.C for 7-10 days and take out; adding in the solid bacterial strain and mix uniformly, fermenting at 25-30 deg.C, and when the mycelium grows completely and drying into the finished products. It simulates the ecological mode of biodegrading stalks in the nature, fully uses specific enzyme system of each bacterial strain, and achieves the purpose of biodegrading stalks.

Description

The method of microbial composite of degrading straw and preparation method thereof and degrading straw
Technical field
The present invention relates to the method for a kind of microbial composite and preparation method thereof and degraded agricultural waste material.
Background technology
Stalk cellulose is a potential feed resource the abundantest on the earth, and the characteristics that its amount of having is big, inexpensive are if it can be degraded to the available feed resource of animal, particularly important for the developing country that has a large population and a few land.
The agricultural crop straw main component is a Mierocrystalline cellulose, hemicellulose and xylogen, and this part accounts for 80% of stalk dry matter weight.Wherein Mierocrystalline cellulose accounts for 30-35%, it be by β-1,4 glucoside bond with the macromolecular compound that the glucose molecule condensation forms, comprise 2000-10000 glucose unit.Natural cellulose exists with linear chain structure, interchain has certain crystal structure and the relatively poor amorphous structure of ordering, many cellulosic molecules are combined into protofibril with setting or amorphous mode again, be interspersed with filler again between many protofibril boundlings or the micro-fibril, i.e. hemicellulose (accounting for the 17-27% of stalk) and xylogen (accounting for the 10-15% of stalk).
The precision architecture of xylogen determines as yet, and existing known its structure is to be the big class biopolymer that the unit constitutes by phenylpropyl alcohol alkane analogue, is the biological high polymer of the difficult degradation of nature.Not only structural unit and the mode that mutually combines are of a great variety for it, but also and combine with different chemical key mode between the Mierocrystalline cellulose, hemicellulose, form very high physical strength, directly influenced the biological degradation of Mierocrystalline cellulose and hemicellulose.
Mierocrystalline cellulose is water insoluble, can only water-swellable, can not be decomposed by the Digestive system and the enzyme of monogastric animal.Because three big non-nutritious matters of stalk respectively have specific enzyme to be and corresponding mechanism of degradation, therefore the microbiological deterioration enzyme system of research destruction stalk imporosity is the maximum technological difficulties of stalk cellulose degraded.
Many bacteriums, actinomycetes, micro-fungus can both produce cellulase in the microorganism, but find the chemical bond that some bacterium and fungi can the cracking xylogen up to the eighties of last century 40-50 age, just make the stalk cellulose Research on degradation enter a new stage.The former positive chapter of 1984 Japanese scholar mulberries has been found a kind of manganiferous lignin peroxidase in the white rot basidiomycetes; The someone finds that a kind of pseudomonas also can produce lignin-degrading enzymes system again subsequently; Britain Aston university research personnel utilize white rot to belong to the research well afoot examination experiment of bacterium degrading straw xylogen, U.S. scientist is cloned into lignin depolymerization carbohydrase gene and cellulose enzyme gene in the yeast cell, makes up the Yeast engineering bacteria that contains lignin depolymerization carbohydrase gene and cellulose enzyme gene.About stalk microbe degraded, though it is a lot of to open up the research of new feed resource, the present domestic real adaptable mature technology that do not have as yet.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, a kind of microbial composite of the straw of degrading is provided;
Another object of the present invention provides a kind of preparation method of microbial composite of the straw of degrading;
The 3rd purpose of the present invention provides a kind of method of using the microbial composite degraded straw of degraded straw.
Technical scheme of the present invention is summarized as follows:
A kind of microbial composite of the straw of degrading, it comprises liquid spawn, solid spawn, described liquid spawn are with: plant lactobacillus Lactobacillus plantarum (Oral-Jensen) Holland (China Committee for Culture Collection of Microorganisms common micro-organisms center-CGMCC)
Streptococcus faecium Streptococcus faecalis Andrewes et Horder (CGMCC)
Candida utilis bacterium Candida utilis (Henneberg) Lodder et Kreger-van Rij (CGMCC)
At following material: 80～150 parts of Tomato juice, 3～8 parts of yeast extract pastes, 3～8 parts of peptones, sugar 5～15 parts, KH 2PO 41～3 part, add water in 1000 parts of substratum of making, cultivated 48 hours, and made every milliliter of substratum total count reach 10 for 30 ℃ 9More than;
Described solid spawn be with:
White-rot fungi White-rot fungi (CGMCC)
Oyster cap fungus Pleurotus ostreatus (CGMCC)
At following material: 71～81 parts of straw powder, Semen Maydis powder or 15～25 parts in wheat bran, KH 2PO 41～3 part, MnSO 40.1～0.3 part, MgSO 47H 21.1～2.1 parts of O, vitamins B 110 -1～10 -4G part, CaCl 20.01～0.08 part, add water, make water content reach 65～70%, 25～27 ℃ of cultivations are covered with to mycelia.
A kind of preparation method of microbial composite of the straw of degrading: it comprises the following steps:
(1) preparation of liquid spawn:
With 80～150 parts of Tomato juice, 3～8 parts of yeast extract pastes, 3～8 parts of peptones, sugar 5～15 parts, KH 2PO 41～3 part, add water to 1000 parts of substratum of making, plant lactobacillus Lactobacillus plantarum (Oral-Jensen) Holland, streptococcus faecium Streptococcus faecalis Andrewes et Horder, Candida utilis bacterium Candida utilis (Henneberg) Lodder et Kreger-van Rij are seeded in the substratum, cultivated 48 hours, and made every milliliter of substratum total count reach 10 for 30 ℃ 9More than, liquid spawn;
(2) preparation of solid spawn:
With 71～81 parts of straw powder, Semen Maydis powder or 15～25 parts in wheat bran, KH 2PO 41～3 part, MnSO 40.1～.3 part, MgSO 47H 21.1～2.1 parts of O, vitamins B 110 -1～10 -4G part, CaCl 20.01～0.08 part, add water, make water content reach 65～70% and make substratum, white-rot fungi White-rot fungi, oyster cap fungus Pleurotus ostreatus are seeded on the substratum, 25～27 ℃ of cultivations are covered with to mycelia, solid spawn.
In the batching of the used substratum of liquid spawn, preferably: 100 parts of Tomato juice, 5 parts of yeast extract pastes, 5 parts of peptones, sugar 10 parts, KH 2PO 42 parts.
In the batching of the used substratum of solid spawn, preferably: 78 parts of straw powder, Semen Maydis powder or 20 parts in wheat bran, KH 2PO 42 parts, MnSO 40.2 part, MgSO 47H 21.6 parts of O, vitamins B 110 -3Part, CaCl 20.05 part.
A kind of method of using the microbial composite degrading straw of degrading straw comprises the steps:
(1) be 1: 15000～25000 to mix by weight with liquid spawn in the microbial composite of degrading straw and agricultural crop straw raw material, described agricultural crop straw raw material is by weight 7～9: 0.5～2.5: 0.5 stalk: Semen Maydis powder: wheat bran;
(2) sodium chloride aqueous solution of adding 0.9% makes water content reach 65～70%;
(3) 25～30 ℃ of sealing and fermenting were taken out in 7～10 days in sealed vessel;
(4) join in the material that step (3) obtained in will degrade solid spawn in the microbial composite of straw of 1: 80～120 ratio, mixing, it is thick to spread out and put on into 15～20cm, and 25～30 ℃ of fermentations treat that mycelia covers with, and can drying be finished product.
The weight ratio of liquid spawn and agricultural crop straw raw material is preferably selected 1: 20000.
The agricultural crop straw raw material is the stalk by weight 8: 1.5: 0.5: Semen Maydis powder: wheat bran.
The adding of solid spawn in the material that step (3) is obtained was than preferably 1: 100.
The present invention compared with prior art has following advantage
1. bacterium-fungi collocation optimum combination: the present invention is made up of 2 strain bacteriums, 1 saccharomycete, 1 strain white-rot fungi, 1 strain oyster cap fungus, this bacterial classification is formed the ecomodel of having simulated the degraded of nature straw biological, the unique enzyme that makes full use of every strain bacterium is to have reached the purpose to the straw biological degraded.
2. two-step fermentation: adopt the anaerobic-aerobic two-step fermentation.The first step anaerobically fermenting not only makes stalk tentatively be degraded, and owing to produce acid, makes the pH value of raw material suitably be reduced to the aerobic bacteria fermentation of second step and has created the condition that suits.
Save energy, reduce cost: the first step anaerobically fermenting, because secluding air and product acid, under low pH value situation, suppress and kill other spoilage organism in the raw material, after the aerobic bacteria inoculation of second step, the dominant bacteria of taking as the leading factor, be able to ramp, effectively prevent to pollute, thereby can remove conventional raw material high-temperature sterilization operation from, save energy, reduce cost.Handle 1 ton of straw feed and only need a small amount of artificial and bacterial classification expense, about about 10 yuan, the straw feed of Chu Liing about 60-70 per ton unit like this, general mixed fodder price 500-700 unit, if in aquaculture, substitute 10-15% fryer or laying-hen compound feed, the 30-40% swine feed, economic benefit can increase greatly.
4. compound method is simple and practical.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
Embodiment 1
1. liquid spawn preparation:
(1) medium preparation: with the 100ml of Tomato juice, yeast extract paste 5g, peptone 5g, sugared 10g, KH 2PO 42g adds water to 1000ml and makes liquid nutrient medium;
(2) strain preparation: plant lactobacillus, streptococcus faecium, Candida utilis bacterium are inoculated in the 1000ml liquid nutrient medium, cultivated 48 hours for 30 ℃, every milliliter of total count reaches 10 9Below promptly become liquid spawn.
2. solid spawn preparation:
(1) medium preparation:, add KH with straw powder 78g Semen Maydis powder or wheat bran 20g 2PO 42.0g MnSO 40.2gMgSO 47H 2O 1.6g vitamins B 110 -3G CaCl 20.05g add entry, make water content reach 65～70%, make solid medium;
(2) strain preparation: white-rot fungi, oyster cap fungus are inoculated in the solid medium, and 25～27 ℃ are cultured to mycelia and cover with and promptly become solid spawn.
Embodiment 2
1. liquid spawn preparation:
(1) medium preparation: with the 100ml of Tomato juice, yeast extract paste 5g, peptone 5g, sugared 10g, KH 2PO 42g adds water to 1000ml and makes liquid nutrient medium;
(2) strain preparation: plant lactobacillus, streptococcus faecium, Candida utilis bacterium are inoculated in the 1000ml liquid nutrient medium, cultivated 48 hours for 30 ℃, every milliliter of total count reaches 10 9Below promptly become liquid spawn.
2. solid spawn preparation:
(1) medium preparation:, add KH with straw powder 78g Semen Maydis powder or wheat bran 20g 2PO 42.0g MnSO 40.2gMgSO 47H 2O 1.6g vitamins B 110 -3G CaCl 20.05g add entry, make water content reach 65～70%, make solid medium;
(2) strain preparation: white-rot fungi, oyster cap fungus are inoculated in the solid medium, and 25～27 ℃ are cultured to mycelia and cover with and promptly become solid spawn.
Embodiment 3
1. liquid spawn preparation:
(1) medium preparation: with the 80ml of Tomato juice, yeast extract paste 3g, peptone 8g, sugared 15g, KH 2PO 41g adds water to 1000ml and makes liquid nutrient medium;
(2) strain preparation: plant lactobacillus, streptococcus faecium, Candida utilis bacterium are inoculated in the 1000ml liquid nutrient medium, cultivated 48 hours for 30 ℃, every milliliter of total count reaches 10 9Below promptly become liquid spawn.
2. solid spawn preparation:
(1) medium preparation:, add KH with straw powder 71g Semen Maydis powder or wheat bran 15g 2PO 41.0g MnSO 40.1gMgSO 47H 2O 1.1g vitamins B 110 -4G CaCl 20.01 g adds entry, makes water content reach 65～70%, makes solid medium;
(2) strain preparation: white-rot fungi, oyster cap fungus are inoculated in the solid medium, and 25～27 ℃ are cultured to mycelia and cover with and promptly become solid spawn.
Embodiment 4
1. liquid spawn preparation:
(1) medium preparation: with the 150ml of Tomato juice, yeast extract paste 8g, peptone 3g, sugared 5g, KH 2PO 43g adds water to 1000ml and makes liquid nutrient medium;
(2) strain preparation: plant lactobacillus, streptococcus faecium, Candida utilis bacterium are inoculated in the 1000ml liquid nutrient medium, cultivated 48 hours for 30 ℃, every milliliter of total count reaches 10 9Below promptly become liquid spawn.
2. solid spawn preparation:
(1) medium preparation:, add KH with straw powder 81g Semen Maydis powder or wheat bran 25g 2PO 43.0g MnSO 40.3gMgSO 47H 2O 2.1g vitamins B 110 -1G CaCl 20.08g add entry, make water content reach 65～70%, make solid medium;
(2) strain preparation: white-rot fungi, oyster cap fungus are inoculated in the solid medium, and 25～27 ℃ are cultured to mycelia and cover with and promptly become solid spawn.
Embodiment 5
Method with the microbial composite degraded straw of degraded straw: liquid spawn 50ml is added in the corn stalk powder of 800 kilograms of pulverizing, also can be straw powder or wheat straw, add 150 kilograms of Semen Maydis powders again, also can be rice bran, 50 kilograms of wheat bran mixings, the salt solution of adding 0.9% makes water content reach 65～70%.Enclose in the cellar for storing things, 25～30 ℃ of sealing and fermenting were taken out the back in 10 days and are added 10 kilograms of solid spawns, spread out into 15～20cm thin layer behind the mixing, 25～30 ℃ of fermentations, treat that it can dryly be finished product that mycelia is covered with, can be distributed into product by specification quantity after the pulverizing, with the mixed fodder of its 10～30% alternative chicken or pig.
Embodiment 6
Method with the microbial composite degraded straw of degraded straw: liquid spawn 50ml is added in the corn stalk powder of 525 kilograms of pulverizing, also can be straw powder or wheat straw, add 187.5 kilograms of Semen Maydis powders again, also can be rice bran, 37.5 kilograms of wheat bran mixings, the salt solution of adding 0.9% makes water content reach 65～70%.Enclose in the cellar for storing things, 25～30 ℃ of sealing and fermenting were taken out the back in 10 days and are added 8 kilograms of solid spawns, spread out into 15～20cm thin layer behind the mixing, 25～30 ℃ of fermentations, treat that it can drying be finished product that mycelia is covered with, can be distributed into product by specification quantity after the pulverizing, with the mixed fodder of its 10～30% alternative chicken or pig.
Embodiment 7
Method with the microbial composite degraded straw of degraded straw: liquid spawn 50ml is added in the corn stalk powder of 1125 kilograms of pulverizing, also can be straw powder or wheat straw, add 62.5 kilograms of Semen Maydis powders again, also can be rice bran, 62.5 kilograms of wheat bran mixings, the salt solution of adding 0.9% makes water content reach 65～70%.Enclose in the cellar for storing things, 25～30 ℃ of sealing and fermenting were taken out the back in 10 days and are added 12 kilograms of solid spawns, spread out into 15～20cm thin layer behind the mixing, 25～30 ℃ of fermentations, treat that it can dryly be finished product that mycelia is covered with, can be distributed into product by specification quantity after the pulverizing, with the mixed fodder of its 10～30% alternative chicken or pig.

Claims (7)

1. the microbial composite of the straw of degrading is characterized in that it comprises liquid spawn, solid spawn, described liquid spawn be with:
Plant lactobacillus Lactobacillus plantarum (Oral-Jensen) Holland
Streptococcus faecium Streptococcus faecalis Andrewes et Horder
Candida utilis bacterium Candida utilis (Henneberg) Lodder et Kreger-van Rij
At following material: 80～150 parts of Tomato juice, 3～8 parts of yeast extract pastes, 3～8 parts of peptones, sugar 5～15 parts, KH 2PO 41～3 part, add water in 1000 parts of substratum of making, cultivated 48 hours, and made every milliliter of substratum total count reach 10 for 30 ℃ 9More than;
Described solid spawn be with:
White-rot fungi White-rot fungi
Oyster cap fungus Pleurotus ostreatus
At following material: 71～81 parts of straw powder, Semen Maydis powder or 15～25 parts in wheat bran, KH 2PO 41～3 part, MnSO 40.1～0.3 part, MgSO 47H 21.1～2.1 parts of O, vitamins B 110 -1～10 -4G part, CaCl 20.01～0.08 part, add water, make water content reach 65～70%, 25～27 ℃ of cultivations are covered with to mycelia.
2. the preparation method of the microbial composite of the straw of degrading: it is characterized in that it comprises the following steps:
(1) preparation of liquid spawn:
With 80～150 parts of Tomato juice, 3～8 parts of yeast extract pastes, 3～8 parts of peptones, sugar 5～15 parts, KH 2PO 41～3 part, add water to 1000 parts of substratum of making, plant lactobacillus Lactobacillus plantarum (Oral-Jensen) Holland, streptococcus faecium Streptococcus faecalis Andrewes et Horder, Candida utilis bacterium Candida utilis (Henneberg) Lodder et Kreger-van Rij are seeded in the substratum, cultivated 48 hours, and made every milliliter of substratum total count reach 10 for 30 ℃ 9More than, liquid spawn;
(2) preparation of solid spawn:
With 71～81 parts of straw powder, Semen Maydis powder or 15～25 parts in wheat bran, KH 2PO 41～3 part, MnSO 40.1～0.3 part, MgSO 47H 21.1～2.1 parts of O, vitamins B 110 -1～10 -4G part, CaCl 20.01～0.08 part, add water, make water content reach 65～70% and make substratum, white-rot fungi White-rot fungi, oyster cap fungus Pleurotusostreatus are seeded on the substratum, 25～27 ℃ of cultivations are covered with to mycelia, solid spawn.
3. the preparation method of the microbial composite of a kind of straw of degrading according to claim 2: it is characterized in that 100 parts of described Tomato juice, 5 parts of yeast extract pastes, 5 parts of peptones, sugar 10 parts, KH 2PO 42 parts; 78 parts of described straw powder, Semen Maydis powder or 20 parts in wheat bran, KH 2PO 42 parts, MnSO 40.2 part, MgSO 47H 21.6 parts of O, vitamins B 110 -3Part, CaCl 20.05 part.
4. the method with the microbial composite degrading straw of degrading straw is characterized in that comprising the steps:
(1) be 1: 15000～25000 to mix by weight with liquid spawn in the microbial composite of degrading straw and agricultural crop straw raw material, described agricultural crop straw raw material is by weight 7～9: 0.5～2.5: 0.5 stalk: Semen Maydis powder: wheat bran;
(2) sodium chloride aqueous solution of adding 0.9% makes water content reach 65～70%;
(3) 25～30 ℃ of sealing and fermenting were taken out in 7～10 days in sealed vessel;
(4) join in the material that step (3) obtained in will degrade solid spawn in the microbial composite of straw of 1: 80～120 ratio, mixing, it is thick to spread out and put on into 15～20cm, and 25～30 ℃ of fermentations treat that mycelia covers with, and can drying be finished product.
5. a kind of method of using the microbial composite degrading straw of degrading straw according to claim 4, the weight ratio that it is characterized in that described liquid spawn and agricultural crop straw raw material is 1: 20000.
6. a kind of method of using the microbial composite degrading straw of degrading straw according to claim 4 is characterized in that described agricultural crop straw raw material is the stalk by weight 8: 1.5: 0.5: Semen Maydis powder: wheat bran.
7. a kind of method of using the microbial composite degrading straw of degrading straw according to claim 4 is characterized in that the adding ratio of described solid spawn in the material that step (3) is obtained is 1: 100.
CNA2003101070709A 2003-11-21 2003-11-21 Microorganism compositions for degrading straw, preparation thereof and method for degradation of straw Pending CN1544610A (en)

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101886042A (en) * 2010-06-29 2010-11-17 中国农业科学院农业资源与农业区划研究所 Method for constructing composite bacteria system for decaying maize straws
CN102178045A (en) * 2011-06-23 2011-09-14 南开大学 Method for producing feed by fermenting and ammoniating straws
US20120004107A1 (en) * 2009-03-23 2012-01-05 Donaghys Industries Limited Bio-Stimulant for Improved Plant Growth and Development
CN102676492A (en) * 2012-05-30 2012-09-19 上海交通大学 Efficient straw degrading composite fungicide and organic fertilizer preparing method thereof
CN102948612A (en) * 2012-12-18 2013-03-06 陈建军 Fodder solid fermenting method
CN104496577A (en) * 2014-12-25 2015-04-08 刘长生 Composite microbial fertilizer and preparation method thereof
CN104649757A (en) * 2015-02-04 2015-05-27 中国烟草总公司广东省公司 Soil high-carbon-based additive prepared from fermented cotton straw
WO2015087143A1 (en) 2013-12-11 2015-06-18 Pontificia Universidad Javeriana Consortium of fungi immobilised on a laminar lignocellulose carrier for the treatment of wastewater and method for producing same
CN107603916A (en) * 2017-10-30 2018-01-19 南京康之春生物科技有限公司 A kind of antimicrobial composition microbial inoculum, its preparation method and application
CN109020637A (en) * 2018-08-03 2018-12-18 淅川县物华生物科技有限公司 A method of organic fertilizer is produced using stalk

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9131700B2 (en) 2009-03-23 2015-09-15 Donaghys Limited Bio-stimulant for improved plant growth and development
US20120004107A1 (en) * 2009-03-23 2012-01-05 Donaghys Industries Limited Bio-Stimulant for Improved Plant Growth and Development
US9770037B2 (en) 2009-03-23 2017-09-26 Donaghys Limited Bio-stimulant for improved plant growth and development
US8709977B2 (en) * 2009-03-23 2014-04-29 Donaghys Industries Limited Bio-stimulant for improved plant growth and development
CN101886042A (en) * 2010-06-29 2010-11-17 中国农业科学院农业资源与农业区划研究所 Method for constructing composite bacteria system for decaying maize straws
CN102178045A (en) * 2011-06-23 2011-09-14 南开大学 Method for producing feed by fermenting and ammoniating straws
CN102676492A (en) * 2012-05-30 2012-09-19 上海交通大学 Efficient straw degrading composite fungicide and organic fertilizer preparing method thereof
CN102948612A (en) * 2012-12-18 2013-03-06 陈建军 Fodder solid fermenting method
WO2015087143A1 (en) 2013-12-11 2015-06-18 Pontificia Universidad Javeriana Consortium of fungi immobilised on a laminar lignocellulose carrier for the treatment of wastewater and method for producing same
CN104496577A (en) * 2014-12-25 2015-04-08 刘长生 Composite microbial fertilizer and preparation method thereof
CN104649757A (en) * 2015-02-04 2015-05-27 中国烟草总公司广东省公司 Soil high-carbon-based additive prepared from fermented cotton straw
CN104649757B (en) * 2015-02-04 2018-03-13 中国烟草总公司广东省公司 The high carbon-based additive of soil that a kind of cotton stalk that ferments is prepared
CN107603916A (en) * 2017-10-30 2018-01-19 南京康之春生物科技有限公司 A kind of antimicrobial composition microbial inoculum, its preparation method and application
CN109020637A (en) * 2018-08-03 2018-12-18 淅川县物华生物科技有限公司 A method of organic fertilizer is produced using stalk

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