CN113652379B - Bacillus cell wall depolymerization GIEC and application thereof - Google Patents
Bacillus cell wall depolymerization GIEC and application thereof Download PDFInfo
- Publication number
- CN113652379B CN113652379B CN202111136119.8A CN202111136119A CN113652379B CN 113652379 B CN113652379 B CN 113652379B CN 202111136119 A CN202111136119 A CN 202111136119A CN 113652379 B CN113652379 B CN 113652379B
- Authority
- CN
- China
- Prior art keywords
- cell wall
- giec
- fermentation
- depolymerization
- equal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000002421 cell wall Anatomy 0.000 title claims abstract description 61
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 16
- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 16
- 229940082787 spirulina Drugs 0.000 claims abstract description 16
- 241000209046 Pennisetum Species 0.000 claims abstract description 14
- 240000008042 Zea mays Species 0.000 claims abstract description 9
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims description 41
- 230000004151 fermentation Effects 0.000 claims description 41
- 239000002028 Biomass Substances 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- 230000000813 microbial effect Effects 0.000 claims description 7
- 230000004913 activation Effects 0.000 claims description 6
- 239000004480 active ingredient Substances 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims 1
- 235000009973 maize Nutrition 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 abstract description 19
- 238000006731 degradation reaction Methods 0.000 abstract description 19
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 8
- 235000005822 corn Nutrition 0.000 abstract description 8
- 150000001720 carbohydrates Chemical class 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 6
- 229920002488 Hemicellulose Polymers 0.000 abstract description 5
- 239000001913 cellulose Substances 0.000 abstract description 5
- 229920002678 cellulose Polymers 0.000 abstract description 5
- 229920005610 lignin Polymers 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 230000000593 degrading effect Effects 0.000 abstract description 4
- 238000011084 recovery Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 28
- 238000011081 inoculation Methods 0.000 description 8
- 238000012216 screening Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000005273 aeration Methods 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 238000007865 diluting Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 241001661790 Buddleja japonica Species 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000002029 lignocellulosic biomass Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 229920001284 acidic polysaccharide Polymers 0.000 description 1
- 150000004805 acidic polysaccharides Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000010907 stover Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses bacillus cell wall depolymerization GIEC and application thereof, belonging to the field of biotechnology, wherein the cell wall depolymerization GIEC is stored in the Guangdong province microorganism strain collection center at 2021, 2 and 5 days, and the storage number is GDMCC No. 61501. According to the invention, a plurality of experiments prove that cell wall depolymerization GIEC can effectively degrade cell walls, for example, spirulina is degraded by using the strain, and the recovery rate of carbohydrate in the obtained depolymerization liquid is more than or equal to 40%; the strain is used for degrading hybrid pennisetum, pennisetum and corn stalks, the carbohydrate in the obtained degradation liquid is more than or equal to 2g/L, the protein is more than or equal to 0.7g/L, the cellulose degradation rate is more than or equal to 17%, the hemicellulose degradation rate is more than or equal to 19%, and the lignin degradation rate is more than or equal to 16%.
Description
Technical Field
The invention relates to the technical field of biology, in particular to bacillus cell wall depolymerization GIEC and application thereof.
Background
Spirulina is a cyanobacteria rich in proteins, polysaccharides, lipids, vitamins and minerals, and also high value substances such as chlorophyll, carotenoids and unsaturated fatty acids. Spirulina has a typical resistant cell wall consisting of multiple layers of dextran and peptidoglycan polymers, covered externally with a sheath of acidic polysaccharide. The cell wall of lignocellulosic biomass is composed of cellulose, hemicellulose, pectin, lignin, cell wall proteins, and other components, between which there are very complex covalent and non-covalent bonds.
Due to the complex structure of the cell wall, the limitation of the cell wall directly leads to poor high-value utilization effect of biomass. In order to realize the recycling of spirulina and lignocellulosic biomass, various pretreatment means are indispensable steps for cell wall disruption.
The pretreatment method mainly comprises a physical method, a chemical method, a physicochemical method and a biological method. At present, most manufacturers at home and abroad pretreat microalgae and lignocellulose biomass by a chemical method. The biggest disadvantage of using chemical pretreatment is that the high temperature of strong alkali can destroy the activity of the endogenous substances of microalgae and generate black liquor in the pretreatment process of the lignocellulose biomass. The principle of the biological fermentation method is that microorganisms are utilized to generate various enzymes in the metabolic process taking spirulina, lignocellulose biomass and the like as nutrients, so that macromolecular substances forming the cell wall are degraded into micromolecules and water-soluble substances. The microbial fermentation does not need energy consumption and high pressure of a physical method, and does not need strong alkali and high temperature of a chemical method, so that the microbial fermentation is a mild pretreatment mode. Accordingly, the present invention is directed to screening for microorganisms capable of efficiently depolymerizing cell walls.
Disclosure of Invention
The first object of the present invention is to provide a bacillusBacillussp.), designated as cell wall depolymerized GIEC, stored in the microorganism culture collection in guangdong, 2 months, 5 days of 2021, at floor 5 of the national institute of advanced middle road 100, university, 5 th floor, in the viewye district, guangdong, and post code: 510070 with deposit number GDMCC No. 61501.
The cell wall depolymerization GIEC has the advantages of 10-18mm colony size, no color, transparency, round shape, neat edge, smooth and moist surface, rod-shaped bacteria observed under a microscope, mesogenic spores and positive gram reaction. And combining with whole genome analysis to identify it as bacillusBacillus). Experiments prove that the cell wall depolymerization GIEC has stronger cell wall depolymerization capability, and particularly can efficiently degrade the complex cell wall structure of the spirulina.
The second object of the present invention is to provide a microbial preparation comprising the culture of cell wall depolymerized GIEC or a fermentation broth thereof as an active ingredient.
A third object of the present invention is to provide the use of the cell wall depolymerizing GIEC or microbial preparation thereof of the present invention for depolymerizing a biomass cell wall;
preferably, the biomass is any one of spirulina, hybrid pennisetum, pennisetum or corn stalks;
further, the cell wall depolymerizing GIEC or the microbial preparation thereof of the present invention is used for depolymerizing biomass cell walls to prepare foods or feeds or additives.
A fourth object of the present invention is to provide a method for depolymerizing a biomass cell wall comprising the steps of:
a. preparing a fermentation medium: adding deionized water into biomass to obtain a required fermentation medium;
b. inoculating and fermenting: the cell wall depolymerized GIEC of the present invention was inoculated into a fermentation medium for fermentation.
Preferably, the ingredients of the fermentation medium are 100-150 parts by weight of biomass, and deionized water is added to 1000 parts by weight;
preferably, the inoculation is to transfer the cell wall depolymerized GIEC from the preserved liquid medium to a fresh liquid medium for activation, provided that: shaking table 37 ℃,150r/min, shake culture for 48h;
preferably, the fermentation conditions are a temperature of 37 ℃, an oscillating rotation speed of 140-150rpm, and a ventilation N 2 :CO 2 =4:1。
Compared with the prior art, the invention has the following beneficial effects:
(1) The bacillus has stronger cell wall depolymerization capability, and the strain is used for degrading spirulina, so that the recovery rate of carbohydrate in the depolymerization liquid is more than or equal to 40%;
(2) The bacillus provided by the invention has stronger cell wall depolymerization capability, and the strain is used for degrading hybrid pennisetum, pennisetum and corn stalks, so that the carbohydrate in the degradation liquid is more than or equal to 2g/L, the protein is more than or equal to 0.7g/L, the cellulose degradation rate is more than or equal to 17%, the hemicellulose degradation rate is more than or equal to 19%, and the lignin degradation rate is more than or equal to 16%.
The invention is characterized in thatBacillus sp, cell wall depolymerization GIEC, stored in Guangdong at 2021, 2 and 5 daysThe provincial microorganism strain collection center has an address of building 5 of No. 59 of Mitsui No. 100 of Xiuzhou district of Guangzhou, guangdong province, and the post code is as follows: 510070 with deposit number GDMCC No. 61501.
Drawings
FIG. 1 is a microscopic view of cell wall depolymerizing GIEC cell morphology (A) and spirulina state (B).
Detailed Description
The following examples are further illustrative of the invention and are not intended to be limiting thereof.
The present invention will be further described with reference to examples, in which the test methods are conventional test methods unless otherwise specified, and the test reagents and consumables described in the examples are from conventional Biochemical reagent company unless otherwise specified.
Example 1 screening and identification of strains
1.1 Screening of bacterial Strain cell wall depolymerizing GIEC
And diluting the probiotic capsules obtained from the market with a proper amount of sterile water, then coating the diluted probiotic capsules on a dilution plate, and selecting the grown bacterial colonies into a liquid culture medium to obtain target bacterial colonies. The liquid culture medium is obtained by diluting spirulina in logarithmic culture period to proper concentration, and sterilizing at 115deg.C for 20min under high pressure; the solid medium was obtained by adding 1% agar to the above liquid medium.
1.2 Identification of bacterial Strain cell wall depolymerizing GIEC
Morphological characteristics of the strain: the colony is 10-18mm in size, colorless, transparent, round, neat in edge, smooth and moist in surface, and the bacteria are observed to be rod-shaped under a microscope, and the spores are mesogenic, so that gram reaction is positive. And combining with whole genome analysis to identify it as bacillusBacillus）。
Thus, the strain is named by combining morphological, molecular biological and physiological biochemical characteristicsBacillus sp, cell wall depolymerization GIEC, stored in the microorganism strain collection center of guangdong, 2 months 5 days 2021, at the address of building 5 of first institute No. 100, the xiu district of guangzhou city, guangdong, post code: 510070 with deposit number GDMCC No. 61501.
EXAMPLE 2 cell wall depolymerization GIEC depolymerization of Spirulina cell wall
(1) Cell wall disaggregation GIEC seed activation
The cell wall depolymerized GIEC obtained by the screening in example 1 was transferred from the preserved liquid medium to a fresh liquid medium, and cultured by shaking at 37℃in a shaker at 150r/min for 48 hours. Seed quality standard: OD value reaches 0.5 when measured at the wavelength of 600nm, and the microscopic bacteria are full. The inoculation amount is 10%. The preserved liquid culture medium is obtained by diluting spirulina in logarithmic culture stage to proper concentration, and sterilizing at 115deg.C for 20min under high pressure.
(2) Preparing fermentation medium
Collecting spirulina (dry weight of about 1.7 mg/ml) in logarithmic growth period to obtain the desired fermentation medium.
(3) Inoculating and fermenting
And (3) inoculating 10% of cell wall depolymerized GIEC into a fermentation medium for fermentation to obtain the biomass degradation liquid. Fermentation parameters were controlled as follows: fermentation temperature: 37 ℃; oscillating rotational speed: 150rpm, aeration rate; n (N) 2 :CO 2 =4:1 (v/v), samples were taken at 0, 3, 6, 24 hours after inoculation, then taken every 24 hours, and the cell morphology and spirulina state were microscopic, and the results are shown in fig. 1. The samples were then centrifuged at 8000rpm for 5min, and the supernatant and the slag were separated and stored separately.
EXAMPLE 3 cell wall depolymerization of GIEC depolymerization of hybrid pennisetum cell walls
(1) Cell wall disaggregation GIEC seed activation
The cell wall depolymerized GIEC obtained by the screening in example 1 was transferred from the preserved liquid medium to a fresh liquid medium, and cultured by shaking at 37℃in a shaker at 150r/min for 48 hours. Seed quality standard: OD (optical density) 600 The value reaches 0.5, and the microscopic bacteria are full. The inoculation amount is 10%.
(2) Crushing hybrid pennisetum
Pulverizing hybrid pennisetum with pulverizer for 10min.
(3) Preparation of fermentation Medium
Taking 100g of crushed hybrid pennisetum, and adding deionized water to 1000ml to obtain the required fermentation medium.
(4) Inoculating and fermenting
Inoculating the cell wall depolymerized GIEC into a fermentation medium for fermentation to obtain the biomass degradation liquid. Fermentation parameters were controlled as follows: fermentation temperature: 37 ℃; oscillating rotational speed: 150rpm, aeration rate; n (N) 2 :CO 2 =4:1 (v/v), samples were taken at 0, 3, 6, 24 hours after inoculation, respectively, and then taken every 24 hours. The sample was then centrifuged for 5min, and the supernatant and the residue were separated and stored separately.
EXAMPLE 4 cell wall depolymerization of GIEC depolymerization of Japanese butterflybush
(1) Cell wall disaggregation GIEC seed activation
The cell wall depolymerized GIEC obtained by the screening in example 1 was transferred from the preserved liquid medium to a fresh liquid medium, and cultured by shaking at 37℃in a shaker at 150r/min for 48 hours. Seed quality standard: OD (optical density) 600 The value reaches 0.5, and the microscopic bacteria are full. The inoculation amount is 10%.
(2) Pulverizing herba Ardisiae Japonicae
Pulverizing herba Ardisiae Japonicae with pulverizer for 10min.
(3) Preparation of fermentation Medium
150g of crushed Japanese butterflybush herb is taken, deionized water is added to 1000ml, and the required fermentation medium is obtained.
(4) Inoculating and fermenting
Inoculating the cell wall depolymerized GIEC into a fermentation medium for fermentation to obtain the biomass degradation liquid. Fermentation parameters were controlled as follows: fermentation temperature: 37 ℃; oscillating rotational speed: 140rpm, aeration rate; n (N) 2 :CO 2 =4:1 (v/v), samples were taken at 0, 3, 6, 24 hours after inoculation, respectively, and then taken every 24 hours. The sample was then centrifuged for 5min, and the supernatant and the residue were separated and stored separately.
Example 5 cell wall depolymerization of GIEC depolymerized corn stover
(1) Cell wall disaggregation GIEC seed activation
The cell wall depolymerized GIEC obtained by the screening in example 1 was transferred from the preserved liquid medium to a fresh liquid medium, and cultured by shaking at 37℃in a shaker at 140r/min for 48 hours. Seed quality standard: OD (optical density) 600 The value reaches 0.5, and the microscopic bacteria are full. Inoculum size 15%。
(2) Corn stalk crushing
The corn stalks are crushed for 10min by a crusher.
(3) Preparation of fermentation Medium
100g of crushed corn straw is taken, deionized water is added to 1000ml, and the required fermentation medium is obtained.
(4) Inoculating and fermenting
Inoculating the cell wall depolymerized GIEC into a fermentation medium for fermentation to obtain the biomass degradation liquid. Fermentation parameters were controlled as follows: fermentation temperature: 37 ℃; oscillating rotational speed: 140rpm, aeration rate; n (N) 2 :CO 2 =4:1 (v/v), samples were taken at 0, 6, 24 hours after inoculation, respectively, and then taken every 24 hours. The sample was then centrifuged for 5min, and the supernatant and the residue were separated and stored separately.
The method is respectively applied to the implementation of the degradation of the spirulina by the strain, and the recovery rate of the carbohydrate in the obtained depolymerization liquid is more than or equal to 40 percent; the strain is used for degrading hybrid pennisetum, pennisetum and corn stalks, and the carbohydrate in the degradation liquid is more than or equal to 2g/L, the protein is more than or equal to 0.7g/L, so that the degradation rate of cellulose in the cell wall is more than 17%, the degradation rate of hemicellulose is more than 19% and the degradation rate of lignin is more than 16%. The degradation rate is calculated as follows:
degradation rate =
Wherein C (r) and C (F) respectively represent the percentage content of cellulose, hemicellulose and lignin before and after bacterial treatment; m (r) and m (F) represent the mass of the solid before and after the treatment, respectively.
Claims (8)
1. Bacillus cellBacillus sp.) cell wall depolymerizing GIEC, characterized in that it has deposit number GDMCC No. 61501.
2. A microbial preparation comprising the fermentation broth of the bacillus cell wall depolymerization GIEC according to claim 1 as an active ingredient.
3. Use of the bacillus cell wall depolymerizing GIEC of claim 1 or the microbial preparation of claim 2 for depolymerizing a biomass cell wall.
4. The use according to claim 3, wherein the biomass is any one of spirulina, hybrid pennisetum, pennisetum or maize straw.
5. A method of depolymerizing a biomass cell wall, comprising the steps of:
a. preparing a fermentation medium: adding deionized water into biomass to obtain a required fermentation medium;
b. inoculating and fermenting: inoculating the bacillus cell wall depolymerized GIEC of claim 1 into a fermentation medium for fermentation.
6. The method according to claim 5, wherein the fermentation medium comprises 100-150 parts by weight biomass and deionized water to 1000 parts by weight.
7. The method of claim 5, wherein the inoculating is performed by transferring the bacillus cell wall depolymerized GIEC of claim 1 from the stored liquid medium to a fresh liquid medium for activation, provided that: shaking table 37 ℃,150r/min, shake culture 48h.
8. The method according to claim 5, wherein the fermentation conditions are a temperature of 37℃and a shaking speed of 140-150rpm, and a ventilation N 2 :CO 2 =4:1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111136119.8A CN113652379B (en) | 2021-09-27 | 2021-09-27 | Bacillus cell wall depolymerization GIEC and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111136119.8A CN113652379B (en) | 2021-09-27 | 2021-09-27 | Bacillus cell wall depolymerization GIEC and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113652379A CN113652379A (en) | 2021-11-16 |
| CN113652379B true CN113652379B (en) | 2023-11-03 |
Family
ID=78494421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111136119.8A Active CN113652379B (en) | 2021-09-27 | 2021-09-27 | Bacillus cell wall depolymerization GIEC and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113652379B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115340964B (en) * | 2022-06-26 | 2024-05-14 | 上海龙殷生物科技有限公司 | Bacillus strain with swollenin activity and application thereof |
| CN115404181A (en) * | 2022-07-25 | 2022-11-29 | 福建华闽晟业生物科技有限公司 | Application of Jujun grass juice as lactobacillus plantarum fermentation medium |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103589670A (en) * | 2013-11-12 | 2014-02-19 | 无锡亚克生物科技有限公司 | Bacillus pumilus GBSW19 and application thereof |
| CN104388341A (en) * | 2014-10-29 | 2015-03-04 | 青岛明月蓝海生物科技有限公司 | Bacillus mucilaginosus strain and application thereof in seaweed degradation |
| CN105385629A (en) * | 2015-12-01 | 2016-03-09 | 北京德瑞丰农业科技有限责任公司 | Lignocellulos substance high-efficiency degrading bacterium M2 and application thereof |
| CN106399156A (en) * | 2016-08-25 | 2017-02-15 | 中国热带农业科学院热带生物技术研究所 | Bacillus amyloliquefaciens subsp.plantarum and application thereof to scagassum biodegradation |
| CN106434417A (en) * | 2016-08-01 | 2017-02-22 | 奥为（天津）环保科技有限公司 | High-temperature-resistant cellulase producing bacterium and application thereof |
| CN109897801A (en) * | 2019-03-15 | 2019-06-18 | 北京天诚众合科技发展有限公司 | Control complex microorganism, preparation method and the application of cyanobacterial bloom |
| CN110055195A (en) * | 2019-04-25 | 2019-07-26 | 内蒙古工业大学 | The microbial strains BF-1801 of one high-efficiency degradation cellulose |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL408834A1 (en) * | 2014-07-11 | 2016-01-18 | Uniwersytet Warszawski | Consortium and a preparation of micro-organisms for catalyzing cellulose hydrolysis, preparation for supplementation of methane fermentation, complex preparation and application as well as method for using them |
-
2021
- 2021-09-27 CN CN202111136119.8A patent/CN113652379B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103589670A (en) * | 2013-11-12 | 2014-02-19 | 无锡亚克生物科技有限公司 | Bacillus pumilus GBSW19 and application thereof |
| CN104388341A (en) * | 2014-10-29 | 2015-03-04 | 青岛明月蓝海生物科技有限公司 | Bacillus mucilaginosus strain and application thereof in seaweed degradation |
| CN105385629A (en) * | 2015-12-01 | 2016-03-09 | 北京德瑞丰农业科技有限责任公司 | Lignocellulos substance high-efficiency degrading bacterium M2 and application thereof |
| CN109482618A (en) * | 2015-12-01 | 2019-03-19 | 北京德瑞丰农业科技有限责任公司 | The purposes of bacillus M2 degradation agricultural wastes |
| CN106434417A (en) * | 2016-08-01 | 2017-02-22 | 奥为（天津）环保科技有限公司 | High-temperature-resistant cellulase producing bacterium and application thereof |
| CN106399156A (en) * | 2016-08-25 | 2017-02-15 | 中国热带农业科学院热带生物技术研究所 | Bacillus amyloliquefaciens subsp.plantarum and application thereof to scagassum biodegradation |
| CN109897801A (en) * | 2019-03-15 | 2019-06-18 | 北京天诚众合科技发展有限公司 | Control complex microorganism, preparation method and the application of cyanobacterial bloom |
| CN110055195A (en) * | 2019-04-25 | 2019-07-26 | 内蒙古工业大学 | The microbial strains BF-1801 of one high-efficiency degradation cellulose |
Non-Patent Citations (2)
| Title |
|---|
| 包怡红等.无.《木聚糖酶工程菌的构建》.2005,第174页. * |
| 赵先美等.无.《生活中的节能减排》.2018,第171页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113652379A (en) | 2021-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chen | Modern solid state fermentation | |
| Hu et al. | Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing | |
| CN101381694B (en) | Bacteria cellulose producing bacteria and method for preparing bacteria cellulose using above bacterial strain | |
| CN113652379B (en) | Bacillus cell wall depolymerization GIEC and application thereof | |
| CN110331109B (en) | Bacillus subtilis and culture method and application thereof | |
| CN105754881B (en) | A kind of Irpex lacteus of degradable lignin and its application | |
| CN111363684B (en) | Composite microbial inoculum for efficiently degrading wood fibers and application thereof in composting | |
| CN107365718B (en) | Bacillus megaterium MYB3 and application thereof in straw fermented feed | |
| CN105154371A (en) | Lactobacillus amylovorus for producing feruloyl esterase and application of lactobacillus amylovorus | |
| CN108546660B (en) | Chitin deacetylase high-yield strain and application thereof | |
| CN116463238B (en) | Bacillus subtilis capable of degrading cellulose and application thereof | |
| CN108865927B (en) | Bacterial strain for low-temperature glycolysis of corn straw and fermentation culture method and application thereof | |
| Shokrkar et al. | Exploring strategies for the use of mixed microalgae in cellulase production and its application for bioethanol production | |
| CN105670966B (en) | High-temperature-resistant garden waste decomposing bacteria ST4 and application thereof | |
| CN105969702A (en) | Serratia marcescens RZ 21-C6 and application thereof | |
| CN110819555B (en) | High-temperature-resistant Brevibacillus reuteri NJAU-N20 for efficiently degrading wood fibers and application thereof | |
| CN102173879B (en) | Method for producing biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residue | |
| CN1544610A (en) | Microorganism compositions for degrading straw, preparation thereof and method for degradation of straw | |
| CN115851501A (en) | Bacillus, microbial preparation, culture method and application | |
| CN116004781A (en) | Method for analyzing microbial population functions and constructing functional microbiome based on metagenomic data mining | |
| CN113122460B (en) | Rice straw degrading bacterium and screening method and application thereof | |
| CN105567608B (en) | High-temperature-resistant garden waste decomposing bacteria ST1 and application thereof | |
| CN112251377B (en) | Bacillus brevis, microbial inoculum and application thereof | |
| Chen et al. | Biotechnology principles of solid state fermentation | |
| CN1834222A (en) | Fetid aspergillic strain and uses |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |