CN105670966B - One plant of high temperature resistant garden waste decomposer ST4 and its application - Google Patents
One plant of high temperature resistant garden waste decomposer ST4 and its application Download PDFInfo
- Publication number
- CN105670966B CN105670966B CN201610119798.0A CN201610119798A CN105670966B CN 105670966 B CN105670966 B CN 105670966B CN 201610119798 A CN201610119798 A CN 201610119798A CN 105670966 B CN105670966 B CN 105670966B
- Authority
- CN
- China
- Prior art keywords
- temperature
- composting
- bacterium solution
- garden waste
- decomposing agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
Abstract
The invention discloses one plant of high-temperature fibre element degradation bacteria Streptomycesalbidoflhaving (Streptomyces albidoflavus), deposit number is CGMCC No.12136.Bacterium of the present invention a large amount of producing enzymes, cellulase activity height can have high temperature resistant, efficient degradation cellulosic nature in 40-70 DEG C of high temperature, can be applied in During High-Temperature Composting system as microbial bacterial agent, be suitable for garden waste compost.
Description
Technical field
The invention belongs to field of environmental biotechnology, and in particular to the plant height effect filtered out from garden waste compost
The thermoduric bacteria of degraded cellulose and its application in garden waste During High-Temperature Composting.
Background technique
Garden waste refers to that ornamental plant withers and falls naturally or manually trim generated deadwood, fallen leaves, grass cuttings, residual flower, tree
Wood and shrub beta pruning and other plant residues etc., main component are cellulose and hemicellulose difficult to degrade.In recent years, China garden
The speed increase of the annual 8%-10% of woods waste brings large drag forces to City Green process, and China handles gardens at present
The mode of waste predominantly burn and fill up, both processing modes not only waste renewable resource also create it is serious
Environmental pollution.Therefore, how garden waste is rationally effectively treated, making its resource utilization is the heat of everybody current common concern
One of point problem.
Organic fertilizer and seedling medium etc. is made by garden waste is decomposed using composting technology, is its resource utilization
One of effective outlet, however lead to heap fertilizer efficiency containing substances difficult to degrade such as a large amount of lignin, celluloses in garden waste
Rate is low, the period is long, greatly limits the recycle value of garden waste.Therefore it needs that specific micro- life is added into compost
Object, to accelerate garden waste digest process.Cellulose-degrading bacteria is that one kind can generate extracellular cellulase, and cellulose is big
Molecule is hydrolyzed into the microorganism of glucose, can be with the degradation speed of accelerating fibers cellulosic material.In nature, the micro- of cellulose is generated
There are many biological species, and study and apply at present more is fungi, such as trichoderma, Penicillium, aspergillus, rhizopus.Bacterium
Research and application with actinomyces is less.Cellulose degradation occurs mainly in the megathermal period in composting process, and fungi mainly exists
Under medium temperature condition, enzyme activity is maximum, and with the rising of composting process temperature, the enzymatic activity of fungi number of viable and its generation drops significantly
Low, which limits the utilizations in compost of cellulase-producing mould.The screening of high-temperature fibre element degradation bacteria and application are
The effective measures to solve the above problems.
Many cellulose-degrading bacterias both for agricultural wastes such as corn stover, straw, wheat straws, it is few specifically for
The screening of the degradation bacteria of garden waste cellulosic material and research on utilization.Chinese patent " a plant height temperature cellulose-degrading bacteria and
It is applied " it (number of patent application: 201410018582.6) discloses one plant and is separated from garden waste megathermal period compost sample
High temperature fiber element degradation bacteria ground bacillus, cellulase activity be 7.8 U/ml, the bacterium be bacterium.About discarded from gardens
The report that high-temperature fibre element degradation actinomyces are separated in object compost is less.
Summary of the invention
The technical problems to be solved by the present invention are: providing one plant of high temperature resistant Streptomycesalbidoflhaving, which can be at 40-70 DEG C
A large amount of producing enzymes in high temperature, cellulase activity is high, has high temperature resistant, efficient degradation cellulosic nature, can be as microorganism
Microbial inoculum is applied in During High-Temperature Composting system, is suitable for garden waste compost.
Present invention provide the technical scheme that one plant of high-temperature fibre element degradation bacteria Streptomycesalbidoflhaving
(Streptomyces albidoflavus) ST4, deposit number is CGMCC No.12136, is preserved in Chinese microorganism strain
Preservation administration committee common micro-organisms center.
Streptomycesalbidoflhaving of the present invention (Streptomyces albidoflavus) ST4 is in 2 months 2016 18
Day, (preservation address was: court, Beijing for the preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC institute
The institute 3 of positive area's North Star West Road 1, postcode: 100101), deposit number is CGMCC No. 12136, is survived through detection.
Streptomycesalbidoflhaving of the present invention (Streptomyces albidoflavus) ST4, it is from Changping County, Beijing area apple
The strain that separates in the sample of orchard garden waste compost high temperature mid-term acquisition, being numbered is bacterial strain ST4, which can be
Cellulase and degraded cellulose are generated under the conditions of 40-70 DEG C.Bacteria colony white when bacterial strain is cultivated in Gao Shi I culture medium, gas
Raw mycelia micro white, substrate mycelium is light yellow, does not generate soluble pigment.Under microscope, thallus is in mycelioid, Gram's staining
It is positive, spore oval, fibrillae of spores is flexible, and 1-3 encloses spiral.In conjunction with bacterium colony morphological features and it is based on bacterial 16 S rDNA
The Phylogenetic Analysis of gene order, be accredited as Streptomycesalbidoflhaving (Streptomyces albidoflavus).
The method of bacterial strain of the present invention production cellulase, by the bacterium be inoculated in fermentation medium (medium component:
CMC-Na 0.5%, peptone 1%, wheat bran 3%, NaCl 2%, KH2PO40.1%g, MgSO4•7H2O 0.02%, (NH4)2SO4
0.3%, pH 7.0-7.6) in, turn to cultivate 3-4d in shaking table in temperature 50 C, rpm150, centrifuging and taking supernatant, measures cellulose
Enzymatic activity.
It is the During High-Temperature Composting decomposing agent of main composting material that the present invention also provides a kind of suitable for garden waste, this is decomposed
Agent is obtained in a manner of liquid fermentation, and bacterial concentration is 5 × 109CFU/mL, wheat bran and maize flour 2:1 mixing are adsorbed as bacterium solution
Agent is mixed with adsorbent 1:1 by bacterium solution, is fabricated to solid-state microbial inoculum.
Meanwhile the present invention also provides the Streptomycesalbidoflhaving (Streptomyces albidoflavus) ST4 bacterial strain
Application in the compost maturity using garden waste as main material, with garden waste for main composting material, addition is dynamic
Object excrement and water, make mixed material carbon-nitrogen ratio 25-40:1, moisture content 50-60%, and solid-state decomposing agent compares by the 5% of weight of material
Example is inoculated into compost material, carries out During High-Temperature Composting.
The invention has the following advantages:
Streptomycesalbidoflhaving of the present invention (Streptomyces albidoflavus) ST4 is from garden waste heap
The strain of degraded cellulose is filtered out in fertilizer, more cellulase can be generated in 40-70 DEG C of temperature range, and enzyme activity is up to
27.58 U/mL have high temperature resistant, efficient degradation cellulosic nature.Enzyme activity is maximum under mesophilic condition for opposite fungi, and bacterium
The lower problem of producing enzyme vigor, Streptomycesalbidoflhaving not only adapts to hot environment, but also can generate higher cellulase, to fiber
Cellulosic material has good degradation capability, so Streptomycesalbidoflhaving is suitable for composting process complex environment.
Solid-state decomposing agent is made in the high-temperature fibre element degradation bacteria that the present invention obtains to be added to based on garden waste
Want in the compost of material, compared with the control not being inoculated with, can be improved compost enter the megathermal period time, extend the megathermal period continue
Time megathermal period temperature reduces composting C/N ratio, to accelerate compost maturity process.It can be answered as microbial bacterial agent
For being suitable for garden waste compost in During High-Temperature Composting system.
The present invention be directed to the decomposing agents of the strain of garden waste composting material screening and preparation, can be discarded for gardens
The resource utilization of object provides a reasonable, effective approach, realizes the purpose for reducing environmental pollution, resource circulation utilization.
Detailed description of the invention
Fig. 1 Streptomycesalbidoflhaving (Streptomyces albidoflavus) isolate and purify picture
Fig. 2 Streptomycesalbidoflhaving of the present invention (Streptomyces albidoflavus) 16S rDNA gene order.
Fig. 3 by Streptomycesalbidoflhaving (Streptomyces albidoflavus) and close bacterial strain 16SrDNA base
Because sequence carries out the phylogenetic tree picture constructed when homology Blast is compared.
Heap temperature changes in Fig. 4 composting process.
Carbon-nitrogen ratio (C/N ratio) changes in Fig. 5 composting process.
Specific embodiment
Come that the present invention is furture elucidated below by the detailed description of specific embodiment, but is not to limit of the invention
System, only illustrates.
The screening of 1 high-temperature fibre element degradation bacteria strains of embodiment
From Changping County, Beijing area apple orchard garden waste compost high temperature initial stage, high temperature mid-term, high temperature post material, Beijing
Sample is acquired in Yanqing Plain afforestation afforestation waste deposit；Above-mentioned fresh sample 10g is weighed to be put in equipped with 10
Grain bead simultaneously fills in the conical flasks of 90 ml sterile waters, is placed in the shaking table of 30 DEG C of 150 rpm and shakes 30 min, makes
Sample sufficiently scatters, and stands enrichment culture for 24 hours at 50 DEG C.With aseptic straw draw 1 ml supernatant be added to containing 9ml without
In the test tube of bacterium water, this is 10-1Sample diluting liquid, then from 10-11ml is taken to be added in 9ml sterile water in sample, this is
10-2Sample diluting liquid, and so on, obtain 10-3、10-4、10-5、10-6Then sample diluting liquid draws 100 μ l with pipettor
10-3、10-4、10-5、10-6 Sample diluting liquid is in (culture medium composition are as follows: K on Cellulose and congo red differential medium2HPO40.5g,
Microcrystalline cellulose 1.88g, MgSO4 0.25g, gelatin 2.0g, Congo red 0.5g, agar 16g, distilled water 1000ml, pH
7.0) dilution, is spread evenly across entire plate with spreader, is placed in 50 DEG C of incubators and cultivates 3 days.It selects in fiber
There is the bacterium colony of obvious transparent circle on the Congo red plate of element, be numbered, then crossed repeatedly and isolate and purify acquisition pure strain,
Strain after separation is connected on inclined-plane, 4 DEG C of preservations carry out subsequent experimental.
By the pure bacterial strain for being preserved in 4 DEG C be transferred to carboxymethyl cellulose culture medium (medium component: CMC-Na 15.0g,
NH4NO31.0g, yeast extract 1.0g, MgSO4•7H2O 0.5g, KH2PO41.0g, distilled water 1000ml, agar 16g, pH
7.0) on plate, then activation culture at 50 DEG C is provoked the single colonie on plate and is transferred on the Congo red plate of cellulose, is placed in
It being cultivated in 50 DEG C of incubators, colony diameter d and transparent loop diameter D is measured after 72h, calculates its ratio H, i.e. H=D/d, H value is bigger,
It is stronger to be worth the larger ability for indicating the bacterial strain decomposition of cellulose.According to formation transparent circle on the Congo red identification culture medium of cellulose
Size primarily determines its cellulase-producing activity.
By aforesaid operations, more plants of cellulose-degrading bacterias are obtained, wherein in Changping County, Beijing area apple orchard garden waste
The strain separated in the sample of compost high temperature mid-term acquisition, is named as ST4, and the micro- life of China was deposited on 2 18th, 2016
Object culture presevation administration committee common micro-organisms center is referred to as CGMCC (unit address: the Chaoyang District, Beijing City North Star
The institute 3 of West Road 1, Institute of Microorganism, Academia Sinica, postcode: 100101), and deposit number CGMCC
No.12136.Optical microscope and Gram's staining identification are carried out to ST4 bacterial strain, the bacteria strain is in Gao Shi I culture medium
Bacteria colony white when culture, aerial hyphae micro white, substrate mycelium is light yellow, does not generate soluble pigment.Under microscope, thallus is in
Mycelioid, Gram's staining are positive, and spore oval, fibrillae of spores is flexible, and 1-3 encloses spiral, see Fig. 1.
2 ST4 bacterial strain molecular biology identification of embodiment
Molecular Identification is carried out to the Streptomycesalbidoflhaving that screening obtains, is followed the steps below: picking bacterium
Single colonie is inoculated in liquid Gao Shi I culture medium, 30 DEG C, 120r/min shaking table shaken cultivation, is taken out and is cultivated in 2d
Liquid, 5000r/min centrifugation 1min takes supernatant, according to bacterial genomes DNA extraction kit (the limited public affairs of Tiangeng biochemical technology
Department provides), extract bacterium colony DNA；Universal primer 27F and 1492R carries out PCR amplification to the DNA of bacteria of extraction；27F
Sequence is 5 '-AGA GTT TGA TCC TGG CTC AG-3 '；1492R sequence is 5 '-AAG GAG GTG ATC CAG CCG
CA-3′；PCR product is subjected to sequence, sequencing result BLAST in NCBI database carries out sequence analysis, and
Carry out tetraploid rice.
16S rDNA gene order (the referring to Fig. 2) length of Streptomycesalbidoflhaving is 1263bp, and gene order is mentioned
It is sent on Genbank, carries out tetraploid rice, then use 6.0 Software on Drawing phylogenetic tree of MEGA, Fig. 3 is seen, thus really
Determine the kind of bacterial strain.The result shows that the sequence and Streptomycesalbidoflhaving (Streptomyces albidoflavus) 16S
RDNA gene order similarity is up to 100%, true in combination with colony morphology characteristic, physiological and biochemical property, thallus microscopic features
Determine ST4 bacterial strain be Streptomycesalbidoflhaving (Streptomyces albidoflavus).
3 Streptomycesalbidoflhaving growth measurement of embodiment
Streptomycesalbidoflhaving ST4 is inoculated into CMC fluid nutrient medium (CMC-Na 15.0g, NH4NO31.0g, yeast extract
1.0g, MgSO40.5g, KH2PO41.0g, distilled water 1000mL), culture solution is taken every 12 h, connection is continuous to be sampled to 120 h, surveys
The OD600 value in fixed each period, using incubation time as abscissa, the OD600 value of each sample point is ordinate, draws the growth of the bacterium
Curve, the i.e. growth measurement of the bacterium.From measurement result it can be seen that 0-24h be period of delay, 24 ~ 72h be logarithmic growth phase, 72 ~
96h is stationary phase, > 96h is decline phase.The growth of the bacterial strain of logarithmic phase is rapid, energetic, therefore later enzymatic production is real
In testing, the fermentation liquid of 48 h of Ying Xuanyong is that seed liquor is inoculated with.
4 Streptomycesalbidoflhaving cellulase-producing vitality test of embodiment
Logarithmic phase Streptomycesalbidoflhaving ST4 is inoculated into fermentation medium (medium component: CMC-Na by 1% inoculum concentration
0.5%, peptone 1%, wheat bran 3%, NaCl 2%, KH2PO40.1%, MgSO4•7H2O 0.02%, (NH4)2SO40.3%), pH
In 7.0-7.6), turn to cultivate 3-4d in shaking table in temperature 50 C, rpm150, culture 8000r/min is centrifuged 5min, is taken
Supernatant is crude enzyme liquid, measures cellulase activity with DNS method.Taking 1 mL supernatant, (blank is distilled with 1 ml in test tube
Water replacement), it is placed in 50 DEG C of water-baths, preheats 2min, the substrate solution that 4 ml have been preheated at 50 DEG C, timing is then added
It is taken out after reaction 5min, 4mL DNS developing solution is added, is placed in after shaking up in boiling water bath and heats 5 min, put immediately after taking-up
Enter cooling in cold bath, be settled to 20ml with distilled water, measures absorbance after mixing at 540 nm wavelength.Reference standard is bent
Convert the producing enzyme vigor of the bacterial strain after line.Enzyme activity unit is according to international unit stipulative definition, i.e., in 1 mL system, 1
Enzyme amount needed for catalyzing cellulose hydrolysis generates 1 μm of ol glucose in min is an enzyme activity unit (U/mL).After measured
The cellulase activity of ST4 bacterial strain is 27.58 U/mL.
The preparation of 5 solid-state decomposing agent of embodiment
(1) bacterial strain activates: take 4 DEG C of preservation inclined plane inoculatings of microorganism of the present invention to high family name I solid plate culture medium,
12h is cultivated in 50 DEG C of permanent case realizes bacterial strain activation.
(2) prepared by seed liquor: strain plate 1 in step (1) through slant activation is seeded to the sterile Gao Shi of 1 L
Seed liquor is obtained after cultivating 12h in I fluid nutrient medium, under the conditions of 50 DEG C of 150rpm shaking tables.
(3) preparation of fermentation seed liquid: above-mentioned seed liquor is by 6-10%(v/v) inoculum concentration be seeded to sterilized fermentation
In tank, expansion fermented and cultured is carried out.Under conditions of temperature 50 C, 120 rpm of frequency of oscillation, it must ferment after cultivating 48h
Seed liquor.
(4) it the preparation of solid-state decomposing agent: is adsorbed after wheat bran is mixed with maize flour according to 2:1 mass ratio as bacterium solution
Bacterium solution obtained in agent, with step (3) is mixed with adsorbent volume mass ratio 1:1 by bacterium solution, is fabricated to solid-state decomposing agent, heap
After setting 1 week, it can be used for During High-Temperature Composting.
The compost effect test of 6 decomposing agent of embodiment
With garden wastes for main composting material, animal wastes and water are added, mixed material carbon-nitrogen ratio 25-40 is made:
1, moisture content 50-60%, solid-state decomposing agent are inoculated into compost material in 5% ratio of weight of material, During High-Temperature Composting are carried out, with not
Add the material of decomposing agent for control, when heap temperature rises to 50 DEG C, start turning, the megathermal period, every turning in 2 days was primary, cooling
Phase, turning was primary weekly, not in turning after temperature drops to 40 DEG C.In composting process, become by the daily temperature of measurement heap body
Change, composting material carbon nitrogen (C/N) is than variation, influence of the investigation addition decomposing agent to garden-waste compost decomposition progress.
Heap temperature variation is as shown in Figure 4 in composting process.As shown in Figure 4, the compost treatment of decomposing agent is inoculated in compost
4d temperature rises to 50 DEG C or more, and 50 DEG C or more of megathermal period continues 23d, and temperature is begun to decline later.And it is not inoculated with
Compost treatment temperature just rise to 50 DEG C or more in compost 4d, the processing than inoculation postpones 1d and reaches 50 DEG C or more high temperature
Phase, and 50 DEG C or more duration megathermal period are 18d, fewer than the processing of inoculation 5d are inoculated with the heap body megathermal period of processing
Maximum temperature illustrates to accelerate compost after being inoculated with decomposing agent and enter time megathermal period and high temperature also above processing is not inoculated with
Duration phase.C/N variation is as shown in Figure 5 in composting process.As shown in Figure 5, with the progress of compost, it is inoculated with decomposing agent and not
The lasting reduction of the processing C/N ratio of inoculation, and thus the processing decline degree being inoculated with illustrates higher than processing is not inoculated with, addition is originally
Decomposing agent made from bacterium can accelerate composting process, improve the decomposed effect of garden waste compost.
Claims (5)
1. one plant of high-temperature fibre element degradation bacteria Streptomycesalbidoflhaving (Streptomyces albidoflavus), deposit number is
CGMCC No.12136。
2. it is a kind of suitable for garden waste be main composting material During High-Temperature Composting decomposing agent, it is characterised in that: the decomposing agent
It is obtained in a manner of liquid fermentation, raw material is bacterium solution and adsorbent, and bacterium solution mixes in proportion with adsorbent, is fabricated to solid-state bacterium
Agent, wherein bacterium solution be Streptomycesalbidoflhaving described in claim 1 (Streptomyces albidoflavus) bacterium solution, absorption
Agent is wheat bran and maize flour.
3. During High-Temperature Composting decomposing agent as claimed in claim 2, it is characterised in that: the bacterial concentration is 5 × 109CFU/mL,
Wheat bran and maize flour 2:1 mixing are used as bacterium solution adsorbent, mix by bacterium solution with adsorbent 1:1, are fabricated to solid-state microbial inoculum.
4. application of the During High-Temperature Composting decomposing agent in compost maturity as described in Claims 2 or 3.
5. application as claimed in claim 4, it is characterised in that: with garden waste for main composting material, add animal excreta
Just and water, make mixed material carbon-nitrogen ratio 25-40:1, moisture content 50-60%, solid-state decomposing agent presses the 2.5%-5% ratio of weight of material
Example is inoculated into compost material, carries out During High-Temperature Composting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610119798.0A CN105670966B (en) | 2016-03-03 | 2016-03-03 | One plant of high temperature resistant garden waste decomposer ST4 and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610119798.0A CN105670966B (en) | 2016-03-03 | 2016-03-03 | One plant of high temperature resistant garden waste decomposer ST4 and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105670966A CN105670966A (en) | 2016-06-15 |
| CN105670966B true CN105670966B (en) | 2019-09-17 |
Family
ID=56306424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610119798.0A Active CN105670966B (en) | 2016-03-03 | 2016-03-03 | One plant of high temperature resistant garden waste decomposer ST4 and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105670966B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106588330A (en) * | 2016-12-09 | 2017-04-26 | 重庆市黑土地白蚁防治有限公司 | Method for reusing garden wastes |
| ES2772548A1 (en) * | 2018-12-20 | 2020-07-07 | Univ Valladolid | RECYCLING OF WASTE FROM THE CEREAL INDUSTRY TO OBTAIN PRODUCTS WITH ANTIMICROBIAL CHARACTERISTICS THROUGH COMPOSTATION (Machine-translation by Google Translate, not legally binding) |
| CN110156501A (en) * | 2019-07-04 | 2019-08-23 | 山西农业大学 | A kind of organic fertilizer and preparation method thereof based on garden waste and mushroom bran production |
| CN111676172B (en) * | 2020-07-15 | 2021-07-13 | 元和生物科技(德州)有限公司 | Multifunctional oligotrophic cellulose degrading bacterium compound microbial inoculum and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820361A (en) * | 2014-01-15 | 2014-05-28 | 深圳市铁汉生态环境股份有限公司 | High-temperature cellulose degradation bacterium and application thereof |
| CN105087416A (en) * | 2014-05-19 | 2015-11-25 | 段一皋 | Biopesticide complex inoculant for root disease prevention and control of crops like cotton and preparation method thereof |
-
2016
- 2016-03-03 CN CN201610119798.0A patent/CN105670966B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820361A (en) * | 2014-01-15 | 2014-05-28 | 深圳市铁汉生态环境股份有限公司 | High-temperature cellulose degradation bacterium and application thereof |
| CN105087416A (en) * | 2014-05-19 | 2015-11-25 | 段一皋 | Biopesticide complex inoculant for root disease prevention and control of crops like cotton and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 1株链霉菌BCG36的鉴定及抗菌活性研究;甘光华 等;《广东农业科学》;20141231(第18期);第66-69页 * |
| 冯红梅 等.园林废弃物堆肥中高温纤维素降解菌的筛选及产酶特性.《中国环境科学学会学术年会论文集》.2015,摘要. * |
| 园林废弃物堆肥中高温纤维素降解菌的筛选及产酶特性;冯红梅 等;《中国环境科学学会学术年会论文集》;20151231;摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105670966A (en) | 2016-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105567612B (en) | A kind of degradation composite bacteria agent preparation of garden waste and application | |
| CN105647832B (en) | One plant of high temperature resistant garden waste decomposer FHM1 and its application | |
| CN106316693B (en) | A kind of biological humic acid fertilizer and preparation method thereof | |
| CN105695367B (en) | A kind of compounding microbial inoculum FX of degrading straw and its application | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN105670966B (en) | One plant of high temperature resistant garden waste decomposer ST4 and its application | |
| CN102174423B (en) | Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof | |
| CN104560816A (en) | Bacillus licheniformis with biomass hydrolase activity and application thereof | |
| CN103160441B (en) | Preparation process for Penicillium oxalicum fermentation broth and application thereof in agriculture | |
| CN107325974A (en) | High plant cell wall degrading activity Rumen Fungi and its application in feed ensiling | |
| CN104498407A (en) | Bacillus licheniformis UTM107 producing high-temperature-resistant keratinase and application thereof | |
| CN105567609B (en) | One plant of high temperature resistant garden waste decomposer ST2 and its application | |
| CN108865927B (en) | Bacterial strain for low-temperature glycolysis of corn straw and fermentation culture method and application thereof | |
| CN108070540A (en) | One plant of Surfactant Producing Microorganism and its application in compost | |
| CN104789492A (en) | Bacillus megaterium strain and application thereof | |
| CN104894025B (en) | A kind of streptomycete bacterial strain and its application | |
| CN107058119A (en) | A kind of method for improving Cordyceps militaris liquid state fermentation production cordycepin and thermostable protein production of enzyme | |
| CN105567608B (en) | One plant of high temperature resistant garden waste decomposer ST1 and its application | |
| CN104762229A (en) | A bacillus subtilis strain and applications thereof | |
| CN104560817A (en) | Thermophilic bacillus licheniformis UTM102 for producing phytase and application of thermophilic bacillus licheniformis UTM102 | |
| CN105567610B (en) | One plant of high temperature resistant garden waste decomposer ST5 and its application | |
| CN105567607B (en) | One plant of high temperature resistant garden waste decomposer ST3 and its application | |
| CN104498408A (en) | Bacillus licheniformis UTM104 for producing pyrethroid hydrolase and application of bacillus licheniformis UTM104 | |
| CN108841743A (en) | Cold ground straw decomposing bacterium bacterial strain and its preparation method and application | |
| CN111575194B (en) | Mixed bacteria for degrading straw and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191105 Address after: 100010 55 dong'anmen st, Dongcheng District, Beijing Patentee after: Beijing encyclopedia Technology Co., Ltd Address before: 100083 Haidian District Qinghua East Road,, Beijing Forestry University, No. 162, Beijing Patentee before: Beijing Forestry University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211013 Address after: Building 2, yard 19, Beiyuan East Road, Chaoyang District, Beijing 100012 Patentee after: Beijing youshengji Ecological Technology Co.,Ltd. Address before: No. 55, dong'anmen street, Dongcheng District, Beijing 100010 Patentee before: Beijing encyclopedia Technology Co.,Ltd. |