CN105567609B - One plant of high temperature resistant garden waste decomposer ST2 and its application - Google Patents
One plant of high temperature resistant garden waste decomposer ST2 and its application Download PDFInfo
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- 239000010921 garden waste Substances 0.000 title claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 42
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- 238000009264 composting Methods 0.000 claims abstract description 29
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 26
- 241000187091 Streptomyces diastaticus Species 0.000 claims abstract description 22
- 230000001580 bacterial Effects 0.000 claims abstract description 21
- 230000015556 catabolic process Effects 0.000 claims abstract description 15
- 230000004059 degradation Effects 0.000 claims abstract description 15
- 238000006731 degradation reaction Methods 0.000 claims abstract description 15
- 241000178287 Streptomyces thermodiastaticus Species 0.000 claims abstract description 14
- 239000000835 fiber Substances 0.000 claims abstract description 9
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses one plant of thermophilic streptomyces diastaticus of high-temperature fibre element degradation bacteria (Streptomyces thermodiastaticus), deposit number is CGMCC No.12134.Bacterium of the present invention a large amount of producing enzymes, cellulase activity height can have high temperature resistant, efficient degradation cellulosic nature in 40-70 DEG C of high temperature, can be applied in During High-Temperature Composting system as microbial bacterial agent, be suitable for garden waste compost.
Description
Technical field
The invention belongs to field of environmental biotechnology, and in particular to the plant height effect filtered out from garden waste compost
The thermoduric bacteria of degraded cellulose and its application in garden waste During High-Temperature Composting.
Background technique
Garden waste refers to that ornamental plant withers and falls naturally or manually trim generated deadwood, fallen leaves, grass cuttings, residual flower, tree
Wood and shrub beta pruning and other plant residues etc., main component are cellulose and hemicellulose difficult to degrade.In recent years, China garden
The speed increase of the annual 8%-10% of woods waste brings large drag forces to City Green process, and China handles gardens at present
The mode of waste predominantly burn and fill up, both processing modes not only waste renewable resource also create it is serious
Environmental pollution.Therefore, how garden waste is rationally effectively treated, making its resource utilization is the heat of everybody current common concern
One of point problem.
Organic fertilizer and seedling medium etc. is made by garden waste is decomposed using composting technology, is its resource utilization
One of effective outlet, however lead to heap fertilizer efficiency containing substances difficult to degrade such as a large amount of lignin, celluloses in garden waste
Rate is low, the period is long, greatly limits the recycle value of garden waste.Therefore it needs that specific micro- life is added into compost
Object, to accelerate garden waste digest process.Cellulose-degrading bacteria is that one kind can generate extracellular cellulase, and cellulose is big
Molecule is hydrolyzed into the microorganism of glucose, can be with the degradation speed of accelerating fibers cellulosic material.In nature, the micro- of cellulose is generated
There are many biological species, and study and apply at present more is fungi, such as trichoderma, Penicillium, aspergillus, rhizopus.Bacterium
Research and application with actinomyces is less.Cellulose degradation occurs mainly in the megathermal period in composting process, and fungi mainly exists
Under medium temperature condition, enzyme activity is maximum, and with the rising of composting process temperature, the enzymatic activity of fungi number of viable and its generation drops significantly
Low, which limits the utilizations in compost of cellulase-producing mould.The screening of high-temperature fibre element degradation bacteria and application are
The effective measures to solve the above problems.
Many cellulose-degrading bacterias both for agricultural wastes such as corn stover, straw, wheat straws, it is few specifically for
The screening of the degradation bacteria of garden waste cellulosic material and research on utilization.Chinese patent " a plant height temperature cellulose-degrading bacteria and
It is applied " it (number of patent application: 201410018582.6) discloses one plant and is separated from garden waste megathermal period compost sample
High temperature fiber element degradation bacteria ground bacillus, cellulase activity be 7.8 U/ml, the bacterium be bacterium.About discarded from gardens
The report that high-temperature fibre element degradation actinomyces are separated in object compost is less.
Summary of the invention
The technical problems to be solved by the present invention are: providing one plant of thermophilic streptomyces diastaticus of high temperature resistant, which can be in 40-
A large amount of producing enzymes in 70 DEG C of high temperature, cellulase activity is high, has high temperature resistant, efficient degradation cellulosic nature, can be as micro-
Bacteria agent is applied in During High-Temperature Composting system, is suitable for garden waste compost.
Present invention provide the technical scheme that one plant of thermophilic streptomyces diastaticus of high-temperature fibre element degradation bacteria
(Streptomyces thermodiastaticus) ST2, deposit number is CGMCC No.12134, is preserved in China Microbiological
Culture presevation administration committee common micro-organisms center.
Thermophilic streptomyces diastaticus of the present invention (Streptomyces thermodiastaticus) ST2 is in 2016
Years 18 days 2 months for the preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC institute (preservation address is:
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode: 100101), deposit number is CGMCC No.12134, through detecting
Survival.
The thermophilic streptomyces diastaticus of the present invention (Streptomyces thermodiastaticus) ST2, it is from Bei Jingchang
The strain separated in the sample of flat area apple orchard garden waste compost high temperature mid-term acquisition, being numbered is bacterial strain ST2, should
Bacterium can generate cellulase and degraded cellulose under the conditions of 40-70 DEG C.Bacterial strain on Gao Shi I culture medium when growing, gas
Raw mycelia is light grey, mycelia multiple-limb, and substrate mycelium lark does not generate soluble pigment, and aerial hyphae branch is raw in gas
There is fibrillae of spores on mycelia.Under microscope, thallus is in mycelioid, and Gram's staining is positive, and spore oval, surface is smooth,
The slightly curved song of fibrillae of spores, in the shape of a spiral.It is sent out in conjunction with bacterium colony morphological features with the system based on bacterial 16 S rDNA gene order
Educate analysis, be accredited as thermophilic streptomyces diastaticus (Streptomyces thermodiastaticus)。
The method of bacterial strain of the present invention production cellulase, by the bacterium be inoculated in fermentation medium (medium component:
CMC-Na 0.5%, peptone 1%, wheat bran 3%, NaCl 2%, KH2PO40.1%g, MgSO4•7H2O 0.02%, (NH4)2SO4
0.3%, pH 7.0-7.6) in, turn to cultivate 3-4d in shaking table in temperature 50 C, rpm150, centrifuging and taking supernatant, measures cellulose
Enzymatic activity.
It is the During High-Temperature Composting decomposing agent of main composting material that the present invention also provides a kind of suitable for garden waste, this is decomposed
Agent is obtained in a manner of liquid fermentation, and bacterial concentration is 5 × 109CFU/mL, wheat bran and maize flour 2:1 mixing are adsorbed as bacterium solution
Agent is mixed with adsorbent 1:1 by bacterium solution, is fabricated to solid-state microbial inoculum.
Meanwhile the present invention also provides the thermophilic streptomyces diastaticus ST2 bacterial strains using garden waste as main material
Compost maturity in application add animal wastes and water with garden waste for main composting material, make mixed material carbon nitrogen
Than for 25-40:1, moisture content 50-60%, solid-state decomposing agent is inoculated into compost material in 5% ratio of weight of material, carry out high
Warm compost.
The invention has the following advantages:
Thermophilic streptomyces diastaticus of the present invention (Streptomyces thermodiastaticus) ST2 is from garden
The strain of degraded cellulose is filtered out in woods castoff compost, and more cellulose can be generated in 40-70 DEG C of temperature range
Enzyme, enzyme activity are up to 63.55 U/mL, have high temperature resistant, efficient degradation cellulosic nature.Opposite fungi enzyme activity under mesophilic condition
Maximum, and the problem that bacterium producing enzyme vigor is lower, thermophilic streptomyces diastaticus had not only adapted to hot environment, but also can generate higher
Cellulase has good degradation capability to cellulosic material, so thermophilic streptomyces diastaticus is suitable for composting process
Complex environment.
Solid-state decomposing agent is made in the high-temperature fibre element degradation bacteria that the present invention obtains to be added to based on garden waste
Want in the compost of material, compared with the control not being inoculated with, can be improved compost enter the megathermal period time, extend the megathermal period continue
Time megathermal period temperature reduces composting C/N ratio, to accelerate compost maturity process.It can be answered as microbial bacterial agent
For being suitable for garden waste compost in During High-Temperature Composting system.
The present invention be directed to the decomposing agents of the strain of garden waste composting material screening and preparation, can be discarded for gardens
The resource utilization of object provides a reasonable, effective approach, realizes the purpose for reducing environmental pollution, resource circulation utilization.
Detailed description of the invention
Fig. 1 is thermophilic streptomyces diastaticus (Streptomyces thermodiastaticus) isolate and purify picture
The thermophilic streptomyces diastaticus of Fig. 2 present invention (Streptomyces thermodiastaticus) 16S rDNA
Gene order.
Fig. 3 by thermophilic streptomyces diastaticus (Streptomyces thermodiastaticus) and close bacterial strain
The gene order of 16SrDNA carries out the phylogenetic tree picture constructed when homology Blast is compared.
Heap temperature changes in Fig. 4 composting process.
Carbon-nitrogen ratio (C/N ratio) changes in Fig. 5 composting process.
Specific embodiment
Come that the present invention is furture elucidated below by the detailed description of specific embodiment, but is not to limit of the invention
System, only illustrates.
The screening of 1 high-temperature fibre element degradation bacteria strains of embodiment
From Changping County, Beijing area apple orchard garden waste compost high temperature initial stage, high temperature mid-term, high temperature post material, Beijing
Sample is acquired in Yanqing Plain afforestation afforestation waste deposit；Above-mentioned fresh sample 10g is weighed to be put in equipped with 10
Grain bead simultaneously fills in the conical flasks of 90 ml sterile waters, is placed in the shaking table of 30 DEG C of 150 rpm and shakes 30 min, makes
Sample sufficiently scatters, and stands enrichment culture for 24 hours at 50 DEG C.1 ml supernatant is drawn with aseptic straw to be added to containing 9ml
In the test tube of sterile water, this is 10-1Sample diluting liquid, then from 10-11ml is taken to be added in 9ml sterile water in sample, this is
It is 10-2Sample diluting liquid, and so on, obtain 10-3、10-4、10-5、10-6Then sample diluting liquid draws 100 with pipettor
The 10 of μ l-3、10-4、10-5、10-6 Sample diluting liquid is in (culture medium composition are as follows: K on Cellulose and congo red differential medium2HPO4
0.5g, microcrystalline cellulose 1.88g, MgSO4 0.25g, gelatin 2.0g, Congo red 0.5g, agar 16g, distilled water
1000ml, pH 7.0), dilution is spread evenly across entire plate with spreader, is placed in 50 DEG C of incubators and cultivates 3 days.
The bacterium colony for having obvious transparent circle on the Congo red plate of cellulose is selected, is numbered, then is crossed to isolate and purify repeatedly and be obtained
Pure strain is obtained, the strain after separation is connected on inclined-plane, 4 DEG C of preservations carry out subsequent experimentals.
By the pure bacterial strain for being preserved in 4 DEG C be transferred to carboxymethyl cellulose culture medium (medium component: CMC-Na 15.0g,
NH4NO31.0g, yeast extract 1.0g, MgSO4•7H2O 0.5g, KH2PO41.0g, distilled water 1000ml, agar 16g, pH
7.0) on plate, then activation culture at 50 DEG C is provoked the single colonie on plate and is transferred on the Congo red plate of cellulose, is placed in
It being cultivated in 50 DEG C of incubators, colony diameter d and transparent loop diameter D is measured after 72h, calculates its ratio H, i.e. H=D/d, H value is bigger,
It is stronger to be worth the larger ability for indicating the bacterial strain decomposition of cellulose.According to formation transparent circle on the Congo red identification culture medium of cellulose
Size primarily determines its cellulase-producing activity.
By aforesaid operations, more plants of cellulose-degrading bacterias are obtained, wherein in Changping County, Beijing area apple orchard garden waste
The strain separated in the sample of compost high temperature mid-term acquisition, is named as ST1, and the micro- life of China was deposited on 2 15th, 2016
Object culture presevation administration committee common micro-organisms center is referred to as CGMCC (unit address: the Chaoyang District, Beijing City North Star
The institute 3 of West Road 1, Institute of Microorganism, Academia Sinica, postcode: 100101), and deposit number CGMCC
No.12134.Optical microscope and Gram's staining identification are carried out to ST2 bacterial strain, are somebody's turn to do when being grown on Gao Shi I culture medium,
Aerial hyphae is light gray, and mycelia multiple-limb, substrate mycelium lark does not generate soluble pigment, aerial hyphae branch, in gas
There is fibrillae of spores on raw mycelia.Under microscope, thallus is in mycelioid, and Gram's staining is positive, spore oval, surface light
Sliding, the slightly curved song of fibrillae of spores is shown in Fig. 1 in the shape of a spiral.
2 ST2 bacterial strain molecular biology identification of embodiment
Molecular Identification is carried out to the thermophilic streptomyces diastaticus that screening obtains, follow the steps below: picking screens bacterium
The single colonie of strain is inoculated in liquid Gao Shi I culture medium, 30 DEG C, 120r/min shaking table shaken cultivation, is taken out in 2d
Culture solution, 5000r/min centrifugation 1min take supernatant, and according to bacterial genomes DNA extraction kit, (Tiangeng biochemical technology has
Limit company provides), extract bacterium colony DNA；Universal primer 27F and 1492R carries out PCR amplification to the DNA of bacteria of extraction；
27F sequence is 5 '-AGA GTT TGA TCC TGG CTC AG-3 '；1492R sequence is 5 '-AAG GAG GTG ATC CAG
CCG CA-3′；PCR product is subjected to sequence, sequencing result BLAST in NCBI database carries out sequence point
Analysis, and carry out tetraploid rice.
16S rDNA gene order (the referring to Fig. 2) length of Potsdam bacillus brevis is 1426bp, by gene sequence
Column are submitted on Genbank, carry out tetraploid rice, are then used 6.0 Software on Drawing phylogenetic tree of MEGA, are seen Fig. 3,
So that it is determined that the kind of bacterial strain.The result shows that the sequence and thermophilic streptomyces diastaticus (Streptomyces thermodiastaticus) 16S rDNA gene order similarity be up to 99%, in combination with colony morphology characteristic, life
Reason biochemical character, thallus microscopic features determine ST1 bacterial strain be thermophilic streptomyces diastaticus (Streptomyces thermodiastaticus)。
The thermophilic streptomyces diastaticus growth measurement of embodiment 3
By thermophilic streptomyces diastaticus (Streptomyces thermodiastaticus) ST2 be inoculated into CMC liquid training
Support (CMC-Na 15.0g, NH in base4NO31.0g, yeast extract 1.0g, MgSO40.5g, KH2PO41.0g, distilled water
1000mL), culture solution being taken every 2h, connection is continuous to be sampled to 48h, the OD600 value in each period is measured, using incubation time as abscissa,
The OD600 value of each sample point is ordinate, draws the growth curve of the bacterium, the i.e. growth measurement of the bacterium.It can be with from measurement result
Find out 0-8h be period of delay, 9 ~ 16h be logarithmic growth phase, 16 ~ be for 24 hours stationary phase, > 26h be decline phase.The bacterium of logarithmic phase
Strain growth it is rapid, energetic therefore later enzymatic production experiment in, 12 hours fermentation liquids of Ying Xuanyong be seed liquor into
Row inoculation.
The thermophilic streptomyces diastaticus cellulase-producing vitality test of embodiment 4
By the thermophilic streptomyces diastaticus ST2 of logarithmic phase by 1% inoculum concentration be inoculated into fermentation medium (medium component:
CMC-Na 0.5%, peptone 1%, wheat bran 3%, NaCl 2%, KH2PO40.1%, MgSO4•7H2O 0.02%, (NH4)2SO4
0.3%), pH 7.0-7.6) in, turn to cultivate 3-4d in shaking table in temperature 50 C, rpm150, culture 8000r/min is centrifuged
5min, taking supernatant is crude enzyme liquid, measures cellulase activity with DNS method.Taking 1 mL supernatant, (blank is with 1 in test tube
The replacement of ml distilled water), it is placed in 50 DEG C of water-baths, preheats 2min, the substrate that 4 ml have been preheated at 50 DEG C is then added
Solution takes out after clock reaction 5min, and 4mL DNS developing solution is added, is placed in boiling water bath after shaking up and heats 5 min, taken
It is immediately placed in cooling in cold bath after out, is settled to 20ml with distilled water, measures absorbance after mixing at 540 nm wavelength.
Convert the producing enzyme vigor of the bacterial strain after reference standard curve.Enzyme activity unit is according to international unit stipulative definition, i.e., in 1 mL
In system, enzyme amount needed for catalyzing cellulose hydrolysis generates 1 μm of ol glucose in 1 min is an enzyme activity unit
(U/mL).The cellulase activity of ST2 bacterial strain is 63.55 U/mL after measured.
The preparation of 5 solid-state decomposing agent of embodiment
(1) bacterial strain activates: take 4 DEG C of preservation inclined plane inoculatings of microorganism of the present invention to high family name I solid plate culture medium,
12h is cultivated in 50 DEG C of permanent case realizes bacterial strain activation.
(2) prepared by seed liquor: strain plate 1 in step (1) through slant activation is seeded to the sterile Gao Shi of 1 L I
In fluid nutrient medium, seed liquor is obtained after 12 h are cultivated under the conditions of 50 DEG C of 150rpm shaking tables.
(3) preparation of fermentation seed liquid: above-mentioned seed liquor is by 6-10%(v/v) inoculum concentration be seeded to sterilized hair
In fermentation tank, expansion fermented and cultured is carried out.Under conditions of temperature 50 C, 120 rpm of frequency of oscillation, it must be sent out after cultivating 48h
Ferment seed liquor.
(4) preparation of solid-state decomposing agent: being used as bacterium solution adsorbent after wheat bran is mixed with maize flour according to 2:1 mass ratio,
With bacterium solution obtained in step (3), is mixed by bacterium solution with adsorbent volume mass ratio 1:1, be fabricated to solid-state decomposing agent, bank up 1
Zhou Hou can be used for During High-Temperature Composting.
The compost effect test of 6 decomposing agent of embodiment
With garden wastes for main composting material, animal wastes and water are added, mixed material carbon-nitrogen ratio 25-40 is made:
1, moisture content 50-60%, solid-state decomposing agent are inoculated into compost material in 5% ratio of weight of material, During High-Temperature Composting are carried out, with not
Add the material of decomposing agent for control, when heap temperature rises to 50 DEG C, start turning, the megathermal period, every turning in 2 days was primary, drop
The warm phase, turning was primary weekly, not in turning after temperature drops to 40 DEG C.In composting process, pass through the daily temperature of measurement heap body
Degree variation, composting material carbon nitrogen (C/N) investigate influence of the addition decomposing agent to garden-waste compost decomposition progress than variation.
Heap temperature variation is as shown in Figure 4 in composting process.As shown in Figure 4, the compost treatment of decomposing agent is inoculated in compost
2d temperature rises to 50 DEG C or more, and 50 DEG C or more of megathermal period continues 25d, and temperature is begun to decline later.And it does not connect
The compost treatment temperature of kind just rises to 50 DEG C or more in compost 3d, and it is high to postpone 50 DEG C of 1d arrival or more than the processing of inoculation
Wen Qi, and 50 DEG C or more duration megathermal period are 20d, fewer than the processing of inoculation 5d, is inoculated with the heap body megathermal period of processing
Maximum temperature also above processing is not inoculated with, illustrate to accelerate compost after being inoculated with decomposing agent and enter time megathermal period, Yi Jigao
Warm duration phase.C/N variation is as shown in Figure 5 in composting process.As shown in Figure 5, with the progress of compost, be inoculated with decomposing agent and
The lasting processing decline degree for reducing, and being inoculated with of the processing C/N ratio not being inoculated with, which is higher than, is not inoculated with processing, thus illustrates, adds
Decomposing agent made from this bacterium can accelerate composting process, improve the decomposed effect of garden waste compost.
Claims (5)
1. one plant of thermophilic streptomyces diastaticus of high-temperature fibre element degradation bacteria (Streptomyces thermodiastaticus),
Deposit number is CGMCC No.12134.
2. it is a kind of suitable for garden waste be main composting material During High-Temperature Composting decomposing agent, it is characterised in that: the decomposing agent
It is obtained in a manner of liquid fermentation, raw material is bacterium solution and adsorbent, and bacterium solution mixes in proportion with adsorbent, is fabricated to solid-state bacterium
Agent, wherein bacterium solution be thermophilic streptomyces diastaticus described in claim 1 (Streptomyces thermodiastaticus)
Bacterium solution, adsorbent are wheat bran and maize flour.
3. During High-Temperature Composting decomposing agent as claimed in claim 2, it is characterised in that: the bacterial concentration is 5 × 109CFU/mL,
Wheat bran and maize flour 2:1 mixing are used as bacterium solution adsorbent, mix by bacterium solution with adsorbent 1:1, are fabricated to solid-state microbial inoculum.
4. application of the During High-Temperature Composting decomposing agent in compost maturity as described in Claims 2 or 3.
5. application as claimed in claim 4, it is characterised in that: with garden waste for main composting material, add animal excreta
Just and water, make mixed material carbon-nitrogen ratio 25-40:1, moisture content 50-60%, solid-state decomposing agent presses the 2.5%-5% ratio of weight of material
Example is inoculated into compost material, carries out During High-Temperature Composting.
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| Title |
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| Cellulases of Thermomonospora fusca and Streptomyces thermodiastaticus;DON L. CRAWFORD et al.;《APPLIED MICROBIOLOGY》;19720731;第24卷(第1期);摘要、第150页左栏最后一段，右栏第1-2段 * |
| DON L. CRAWFORD et al..Cellulases of Thermomonospora fusca and Streptomyces thermodiastaticus.《APPLIED MICROBIOLOGY》.1972,第24卷(第1期),摘要、第150页左栏最后一段，右栏第1-2段. * |
| 园林废弃物堆肥中高温纤维素降解菌的筛选及产酶特性;冯红梅 等;《中国环境科学学会学术年会论文集》;20151231;第4150-4156页 * |
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