CN101638328B - Straw organic fertilizer and preparation method thereof - Google Patents
Straw organic fertilizer and preparation method thereof Download PDFInfo
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- CN101638328B CN101638328B CN2008102242457A CN200810224245A CN101638328B CN 101638328 B CN101638328 B CN 101638328B CN 2008102242457 A CN2008102242457 A CN 2008102242457A CN 200810224245 A CN200810224245 A CN 200810224245A CN 101638328 B CN101638328 B CN 101638328B
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Abstract
The invention discloses a straw organic fertilizer and a preparation method thereof. The preparation method is to use a microbial inoculum used for degrading straw to ferment straw so as to obtain the fertilizer; the active ingredients of the microbial inoculum for degrading straw comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratioof Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The fertilizer used for degrading straw can be used as a base fertilizer and can obviously improve the quality of vegetables.
Description
Technical field
The present invention relates to straw organic fertilizer and preparation method thereof.
Background technology
Agricultural crop straw is the non-competing resource of a kind of potential, have quantity big, distribute wide and characteristics such as kind is many.It is reported that China's all kinds of agricultural crop straw gross annual output amount reaches more than 700,000,000 tons, wherein straw is 2.3 hundred million tons, 2.2 hundred million tons of corn straws, and 1,000,000,000 tons of beans and autumn coarse cereals stalks, 1.0 hundred million tons of peanut, potato class tendril and beet leavess etc. account for about 30% of the total stalk amount in the world.But these stalk resources are not but reasonably developed for a long time, and traditionally, stalk is used as the roughage that peasant household produces fuel or is used for livestock, is used for papermaking on a small quantity.In recent years, because peasant household's life fuel falls sharply, straw directly returning to field or become thoroughly decomposed that also the field amount is few, and directly burning amount of field is big, the whole nation is annual to have 2/3 stalk directly to be burned approximately.Only account for 2.6% through what utilize after the technical finesse in all stalks.
Carbon accounts for the overwhelming majority in the stalk, is potassium, silicon, calcium, magnesium, sulphur, phosphorus secondly, and the product after the burning mainly contains CO
2, CO, SO
2, NO
2And N
2O, these obnoxious flavoures have caused the serious environmental pollution after getting into atmosphere.If the field is gone back in these rotten backs of stalk heap, the fertilizer fertilizing soil with becoming horn of plenty improves soil property.
Under the state of nature, cellulose degradation is mainly through three kinds of modes:
1) physics mode comprises pulverizing, boiling, heat spray etc.; 2) chemical mode comprises acidic hydrolysis, alkaline hydrolysis, ammonification and oxidative degradation; 3) microbiological deterioration.Physics or chemical process are handled stalk, can only slightly improve the digestibility of semicellulose, and be little to the effect of Mierocrystalline cellulose and xylogen, fails to improve the nutritive value and the digestibility of stalk.Chemical process can be destroyed xylogen and combine with ester bond between the polysaccharide; Make Mierocrystalline cellulose, semicellulose and lignin separation, insoluble xylogen is become more diffluent hydroxyl xylogen, make the stalk structure become loose; Crystalline cellulose becomes unformed Mierocrystalline cellulose, and then makes that stalk is easy to decompose.But the shortcoming of chemical process is: content of cellulose is too high, protein contnt low (about 5%-10%); Content of mineral substances is uneven, the A that is deficient in vitamin, vitamins D and vitamin E etc.; Low (Qian Huiyue, 2004 of waiting of Energy value; Yang Lianyu, 2001).Mikrobe is through being secreted into the outer free cellulose enzyme of born of the same parents, with lytic enzyme mechanism and oxydase mechanisms of degradation Mierocrystalline cellulose (Zhang Ying, 2005) to cellulosic degraded.Utilize mikrobe can Mierocrystalline cellulose finally be decomposed into carbonic acid gas and water; And can produce a large amount of tropinas through the growth of mikrobe self; Therefore use microbial method to handle stalk; Can increase substantially its nutritive value, compare, have many unrivaled advantages with physico-chemical process.
Productive rate and the activity of utilizing hybrid bacterial strain fermentation can raising cellulase have been reported the seventies in last century.In China, utilize the hybrid bacterial strain fermentation to improve the productive rate and active investigator's the extensive concern that also causes of cellulase.
Summary of the invention
The purpose of this invention is to provide a kind of straw organic fertilizer and preparation method thereof.
The preparation method of straw organic fertilizer provided by the present invention is that the bacteria fermentation stalk with degrading straw obtains fertilizer; The active ingredient of the microbial inoculum of said degrading straw comprises viride (Trichoderma viride), yellow green trichoderma (Trichoderma aureoviride) and feed series bacillus (Paenibacillus pabuli).
The active ingredient of the microbial inoculum of degrading straw provided by the present invention also can only have by described viride (Trichoderma viride), yellow green trichoderma (Trichoderma aureoviride) and feed series bacillus (Paenibacillus pabuli) to be formed.
The microbial inoculum of said degrading straw can be liquid preparation, also can be solid preparation.In liquid preparation, add sorbent material and can obtain solid preparation, as adding the light calcium carbonate or the peat composed of rotten mosses etc.
Wherein, In the microbial inoculum of said degrading straw, the colony forming unit number of said viride (Trichoderma viride), said yellow green trichoderma (Trichoderma aureoviride) and said feed series bacillus (Paenibacilluspabuli) is than being (0.8-1.2): (0.8-1.2): (0.8-1.2).Said viride (Trichoderma viride) is viride (Trichoderma viride) ACCC30166; Said yellow green trichoderma (Trichoderma aureoviride) is yellow green trichoderma (Trichoderma aureoviride) ACCC32248, and said feed series bacillus (Paenibacillus pabuli) is feed series bacillus (Paenibacillus pabuli) ACCC10273.
In the microbial inoculum of said degrading straw; Said viride (Trichoderma viride) ACCC30166, said yellow green trichoderma (Trichoderma aureoviride) ACCC32248 and said feed series bacillus (Paenibacillus pabuli) ACCC10273 can distinguish independent packaging, also can mix.
When said viride (Trichoderma viride) ACCC30166, said yellow green trichoderma (Trichoderma aureoviride) ACCC32248 and the independent packaging of said feed series bacillus (Paenibacilluspabuli) ACCC10273 difference; In the said method; Said yellow green trichoderma (Trichodermaaureoviride) ACCC32248 and said feed series bacillus (Paenibacillus pabuli) ACCC10273 insert in the stalk; After compost fermentation 1-2 days; Insert said viride (Trichodermaviride) ACCC30166 then, fermented again 20-30 days.The access amount of said yellow green trichoderma (Trichoderma aureoviride) ACCC32248 is 1 * 10
4-1 * 10
5Spore count/kg stalk; The access amount of said feed series bacillus (Paenibacillus pabuli) ACCC10273 is 1 * 10
4-1 * 10
5Spore count/kg stalk; The access amount of said viride (Trichoderma viride) ACCC30166 is 1 * 10
4-1 * 10
5Spore count/kg stalk.
The access amount of said viride (Trichoderma viride) ACCC30166 is 1 * 10
4-1 * 10
5Spore count/kg stalk.
When said viride (Trichoderma viride) ACCC30166, said yellow green trichoderma (Trichoderma aureoviride) ACCC32248 and said feed series bacillus (Paenibacilluspabuli) when ACCC10273 mixes; In the said method; The microbial inoculum of said degrading straw inserts in the stalk, ferments 20-30 days.The microbial inoculum access amount of said degrading straw is 1 * 10
4-1 * 10
5Yellow green trichoderma (Trichodermaaureoviride) ACCC32248 spore/kg stalk.
The method that degrading straw of the present invention prepares fertilizer can prepare fertilizer by the quick composting stalk, shifts to an earlier date 15 days at least than the rotten fermentation time of normal heap.Use in the soil of fertilizer of the present invention preparation alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium and organic content all than direct use stalk with do not use the soil of fertilizer high; The fertilizer of the present invention's preparation is as base manure; Can significantly improve the quality of vegetables, use the rape and the Chinese cabbage of the fertilizer of the present invention's preparation, its chlorophyll content can maintain higher level; Compare with Chinese cabbage with the rape of directly using stalk, growth rape in mid-term and Chinese cabbage chlorophyll content improve 25.99% and 10.98% respectively; Can promote simultaneously rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, compare with Chinese cabbage that rape and Chinese cabbage output improve 22.97% and 2.80% respectively with the rape of directly using stalk; And rape reducing sugar and Vc content improve 21.8% and 6.89% respectively; Chinese cabbage reducing sugar and Vc content improve 9% and 23.53% respectively.
Embodiment
The following used bacterial classification of embodiment comes from INST OF AGRICULTURAL RESOURCES Chinese agriculture microbial strains preservation administrative center and (is called for short ACCC; Address: No.12 ,zhongguancun south street,Haidian District, Beijing; INST OF AGRICULTURAL RESOURCES, postcode 100081).
Embodiment 1, degrading straw prepare fertilizer
1) preparation of the microbial inoculum of degrading straw
The microbial inoculum of degrading straw is made up of reagent A, reagent B and reagent H, reagent A, reagent B and reagent H independent packaging.Reagent A, reagent B and reagent H prepare according to following method:
Strain fermentation substratum: Semen Maydis powder 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, 4ml/100ml Vogel ' s mother liquor, pH value 8.5.
Viride (Trichoderma viride) ACCC30166, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent A.
Yellow green trichoderma (Trichoderma aureoviride) ACCC32248, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent B.
Feed series bacillus (Paenibacillus pabuli) ACCC10273 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent H.
Viride in the reagent A (Trichoderma viride) ACCC30166; The colony forming unit number of feed series bacillus (Paenibacillus pabuli) is than being 1:1:1 among (Trichoderma aureo viride) ACCC32248 of yellow green trichoderma among the reagent B and the reagent H.
2) cellulase activity, filter paper enzyme activity and the beta-glucosidase that are used for the microbial inoculum of degrading straw are lived
Mensuration is used for microbial inoculum cellulase activity, filter paper enzyme activity and the beta-glucosidase of degrading straw and lives.
It is following that the DNS reducing sugar method is measured the cellulase activating method: at first adopt 3, and 5-dinitrosalicylic acid (DNS), the 550nm place measures absorbancy, with dextrose anhydrous gradient solution drawing standard curve; (represent restriction endonuclease to live with CMC enzyme activity (CMCase activity); 1.0% carboxymethylcellulose sodium solution of getting the acetum preparation of 1.5mL0.05mol/L sodium-acetate adds the suitable dilution of 0.5mL and falls the microbial inoculum that is used to separate stalk; Add 3mL DNS reagent behind 50 ℃ of reaction 30min; Boiling water bath 5min, cooling back thin up is surveyed reducing sugar to 25mL at 550nm.
Filter paper enzyme activity (filter paper activity; FPA) measuring method is following: the acetum of getting the sodium-acetate of 1.5mL0.05mol/L adds the 1.5mL suitably microbial inoculum that is used for degrading straw and 1 WhatmanNo.1 filter paper bar (about 50mg of dilution; 1cm * 6cm), add 3mL DNS reagent, boiling water bath 5min behind 50 ℃ of insulation 60min; Cooling back thin up is surveyed reducing sugar to 25mL at 550nm.
The measuring method of beta-glucoside enzyme activity is following: the 1.0% saligenin solution of getting the acetum preparation of 1.5mL0.05mol/L sodium-acetate adds the suitably microbial inoculum that is used for degrading straw of dilution of 0.5mL; Add 3mL DNS reagent behind 50 ℃ of reaction 30min; Boiling water bath 5min; Cool off the back thin up to 25mL, survey reducing sugar, calculate enzyme numerical value alive according to the typical curve of drawing then at 550nm.
Mensuration result shows that the CMC enzyme (CMCA) alive that is used for the microbial inoculum of degrading straw reaches 3813.06U/mL, and filter paper enzyme activity (FPA) reaches 2107.57U/mL, and beta-glucosidase reaches 3227.17U/mL.
Above-mentioned experimental result is explained the mutual supplement with each other's advantages that not only has FPA enzyme and beta-glucosidase between three bacterial strains; And reduced whole Mierocrystalline cellulose enzyme to the dependency of nutritive substance and the susceptibility of meta-bolites inhibition, make enzyme activity keep high level in a long time.
3) preparation straw organic fertilizer
Corn straw is pure with the disintegrating machine powder, and the stalk fragment of acquisition is less than 1.0cm, as handled thing.Ratio according to 15kg urea/ton stalk in handled thing adds urea, according to 1 * 10
5Yellow green trichoderma (Trichodermaaureoviride) ACCC32248 of cfu spore/kg stalk inserts the reagent B of embodiment 1; Then according to 1 * 10
5The reagent H that cfu feed series bacillus (Paenibacillus pabuli) ACCC10273 spore/kg stalk inserts embodiment 1 fermented 1.5 days; At last, according to 1 * 10
5Cfu viride (Trichoderma viride) ACCC30166 spore/kg stalk inserts the reagent A of embodiment 1, and fermentation 25d makes straw organic fertilizer 1.
3) step 2) water-soluble carbon (WSC) of the straw organic fertilizer 1 of preparation and total organic nitrogen are measured
Straw organic fertilizer 1 total organic nitrogen is measured and is adopted H
2SO
4-H
2O
2Disappear and boil, nitrogen determination, the WSC of straw organic fertilizer 1 (water-soluble carbon) are measured and are adopted potassium bichromate titrimetric method.Experiment repetition 3 times.
The mensuration result of WSC and total organic nitrogen shows; The WSC of straw organic fertilizer 1 (water-soluble carbon) is 6.42g/kg, and the ratio of WSC and total organic nitrogen is 0.71, and T value (ratio of terminal point C/N and initial C/N) is 0.44; Reach the requirement of becoming thoroughly decomposed, shift to an earlier date 15 days at least than the rotten fermentation time of normal heap.Above-mentioned numerical value is multiple MV 3 times.
Embodiment 2, degrading straw prepare fertilizer
1) preparation of the microbial inoculum of degrading straw
The microbial inoculum of degrading straw is made up of reagent A, reagent B and reagent H, and reagent A, reagent B and reagent H mix the microbial inoculum of processing degrading straw.
Reagent A, reagent B and reagent H prepare according to following method:
Strain fermentation substratum: Semen Maydis powder 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, 4ml/100ml Vogel ' s mother liquor, pH value 8.5.
Viride (Trichoderma viride) ACCC30166, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent A.
Yellow green trichoderma (Trichoderma aureoviride) ACCC32248, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent B.
Feed series bacillus (Paenibacillus pabuli) ACCC10273.In the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 4d collects all fermented liquids as reagent H.
The colony forming unit number of the microbial inoculum viride that is used for degrading straw (Trichoderma viride) ACCC30166 that makes after reagent A, reagent B and reagent H mix, yellow green trichoderma (Trichoderma aureoviride) ACCC32248 and feed series bacillus (Paenibacillus pabuli) ACCC10273 is than being 0.8:0.8:1.2.
2) preparation organic fertilizer
Wheat stalk is pure with the disintegrating machine powder, and the stalk fragment of acquisition is less than 1.0cm, as handled thing.Ratio according to 15kg urea/ton stalk in handled thing adds urea, according to 1.0 * 10
4Yellow green trichoderma (Trichoderma aureoviride) ACCC32248 of cfu spore/kg stalk or according to 1.5 * 10
4Cfu feed series bacillus (Paenibacillus pabuli) ACCC10273 spore/kg stalk or according to 1.0 * 10
4Cfu viride (Trichoderma viride) ACCC30166 spore/kg stalk inserts the bacteria fermentation 30d that is used for degrading straw of the preparation of embodiment 2, makes straw organic fertilizer 2.
3) step 2) WSC of the organic fertilizer 2 of preparation and total organic nitrogen are measured
The WSC of straw organic fertilizer and total organic nitrogen measuring method are with embodiment 1.Experiment repetition 3 times.
The mensuration result of WSC and total organic nitrogen shows; The WSC of organic fertilizer 2 (water-soluble carbon) is 6.40g/kg, and the ratio of WSC and total organic nitrogen is 0.70, and T value (ratio of terminal point C/N and initial C/N) is 0.48; Reach the requirement of becoming thoroughly decomposed, shift to an earlier date 10 days at least than the rotten fermentation time of normal heap.Above-mentioned numerical value is multiple MV 3 times.
Embodiment 3, degrading straw prepare fertilizer
1) preparation of the microbial inoculum of degrading straw
The microbial inoculum of degrading straw is made up of reagent A, reagent B and reagent H, reagent A, reagent B and reagent H independent packaging respectively.Reagent A and reagent B prepare according to following method:
Trichoderma strain fermention medium: Semen Maydis powder 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, 4ml/100ml Vogel ' s mother liquor, pH value 8.5.
Viride (Trichoderma viride) ACCC30166, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 5d collects all fermented liquids as reagent A.
Yellow green trichoderma (Trichoderma aureoviride) ACCC32248, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 5d collects all fermented liquids as reagent B.
Feed series bacillus (Paenibacillus pabuli) ACCC10273.In the trichoderma strain fermention medium 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent H.
The colony forming unit number of the microbial inoculum viride that is used for degrading straw (Trichoderma viride) ACCC30166 that makes after reagent A, reagent B and reagent H mix, yellow green trichoderma (Trichoderma aureoviride) ACCC32248 and feed series bacillus (Paenibacillus pabuli) ACCC10273 compares 1.2:1.2:0.8.
2) preparation organic fertilizer
Corn straw is pure with the disintegrating machine powder, and the stalk fragment of acquisition is less than 1.0cm, as handled thing.Ratio according to 15kg urea/ton stalk in handled thing adds urea, according to 1.5 * 10
4Yellow green trichoderma (Trichoderma aureoviride) ACCC32248 of cfu spore/kg stalk inserts the reagent B of embodiment 1; Then according to 1.0 * 10
5The reagent H that cfu feed series bacillus (Paenibacillus pabuli) ACCC10273 spore/kg stalk inserts embodiment 1 fermented 2 days; At last according to 1.5 * 10
4Cfu viride (Trichodermaviride) ACCC30166 spore/kg stalk inserts the reagent A of embodiment 1, and fermentation 20d makes straw organic fertilizer 3.
3) step 2) WSC of the organic fertilizer 3 of preparation and total organic nitrogen are measured
The measuring method of WSC and total organic nitrogen is with embodiment 1.Experiment repetition 3 times.
The WSC of straw organic fertilizer 3 (water-soluble carbon) is 5.82g/kg, and the ratio of WSC and total organic nitrogen is that 0.75T value (ratio of terminal point C/N and initial C/N) is 0.42, reaches the requirement of becoming thoroughly decomposed, and shifts to an earlier date 20 days at least than the rotten fermentation time of normal heap.
The field experiment of embodiment 4, organic fertilizer
Field experiment is selected rape and Chinese cabbage field respectively; Adopt the completely random block design, 3 district's groups, each district's group is established 5 sub-districts; Test group is that straw organic fertilizer 1,2 and 3 was used as base manure in sowing time or transplanting phase; Contrast 1 is for directly using stalk, and contrast 2 is not for using any fertilizer, and every sub-district area is 15 square metres.
Contrast and test group except that apply fertilizer different, other field management are all identical.
After transplanting 40 days, measure alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium and organic content and soil urease liveness and cellulase activity in the soil.
Measure the soil alkali-hydrolyzable nitrogen with the alkaline hydrolysis diffusion process; Use 0.5molL
-1NaHCO
3Method is measured soil quick-effective phosphor; Use dense H
2SO
4-K
2Cr
2O
4Volumetry-outer heating method is measured soil organic matter content; With DNS reduction colorimetric method for determining cellulase activity; With sodium phenylate colorimetric method for determining urease activity.
Mensuration result shows; Alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium are compared with contrast 2 with organic content and are improved 7.38 ± 1.54mg/kg, 8.52 ± 2.36mg/kg, 15.30 ± 5.60mg/kg respectively in the soil of use straw organic fertilizer 1; 1.1g/kg ± 0.43; Soil urease liveness is compared average raising 14.38 ± 1.35% with contrast 2, and cellulase activity is compared average raising 103.61 ± 12.58% with contrast 2, and comparison is high by 49.23 ± 6.32% according to 1.
Alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium are compared with contrast 2 with organic content and are improved 7.08 ± 2.40mg/kg, 8.00 ± 1.58mg/kg, 13.25 ± 4.56mg/kg respectively in the soil of use straw organic fertilizer 2; 1.31 ± 0.15mg/kg; Soil urease liveness is compared average raising 15.00 ± 3.65% with contrast 2; Cellulase activity is compared average raising 106.37 ± 5.84% with contrast 2, and comparison is high by 55.45 ± 7.56% according to 1.
Alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium are compared with contrast 2 with organic content and are improved 6.23 ± 1.50mg/kg, 6.78 ± 1.84mg/kg, 10.34 ± 5.21mg/kg respectively in the soil of use straw organic fertilizer 3; 1.76 ± 0.62mg/kg; Soil urease liveness is compared average raising 16 ± 2.35% with contrast 2; Cellulase activity is compared average raising 95.37 ± 13.42% with contrast 2, and comparison is high by 56.47 ± 9.65% according to 1.
Growing mid-term, measuring rape and Chinese cabbage chlorophyll; After transplanting 26 days, measure reducing sugar and the Vc content of rape and Chinese cabbage; Measure the output of rape and Chinese cabbage in harvesting time, the experiment triplicate.
Chlorophyll measuring method is following:
Get fresh rape and the cabbage leaf of removing vein respectively; Shred and place 20ml tool plug graduated tube, add 1: 1 by volume blended mixed solution of ethanol and acetone, 30 ℃ of soaked overnight; Measure the vat liquor absorbancy at 440nm, 644nm, 662nm place respectively, according to Ca=9.784 * D
662-0.990 * D
644, Cb=21.426 * D
644-4.650 * D
662, Ca+b=5.134 * D
662+ 20.436 * D
644, Cc=4.695 * D
440-0.268 * (Ca+Cb) waits formula to calculate chlorophyll content (mg/L).Calculate chlorophyll content (mg/L).
Reducing sugar test adopts anthracene copper colourimetry.
The Vc assay adopts 2,6-dichloroindophenol volumetry.
Experimental result shows that straw organic fertilizer 1 is manured into soil and can impels rape and Chinese cabbage chlorophyll content can maintain higher level, compares with contrast 1, and growth rape in mid-term and Chinese cabbage chlorophyll content improve 25.99 ± 2.36% and 10.98 ± 1.48% respectively; Straw organic fertilizer 1 is manured into soil and can promotes rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, and compares with contrast 2, and rape and Chinese cabbage output improve 22.97 ± 3.34% and 2.80 ± 1.03% respectively; Straw organic fertilizer 1 is manured into soil and contrasts 2 and compare, and rape reducing sugar and Vc content improve 21.8 ± 2.85% and 6.89 ± 1.52% respectively; Chinese cabbage reducing sugar and Vc content improve 9 ± 0.58% and 23.53 ± 3.59% respectively.
Straw organic fertilizer 2 is manured into soil and can impels rape and Chinese cabbage chlorophyll content can maintain higher level, compares with contrast 1, and growth rape in mid-term and Chinese cabbage chlorophyll content improve 26.45 ± 4.07% and 11.89 ± 2.36% respectively; Straw organic fertilizer 2 is manured into soil and can promotes rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, and compares with contrast 2, and rape and Chinese cabbage output improve 24.05 ± 1.29% and 3.25 ± 1.30% respectively; Straw organic fertilizer 2 is manured into soil and contrasts 2 and compare, and rape reducing sugar and Vc content improve 25.30 ± 2.87% and 8.03 ± 1.29% respectively; Chinese cabbage reducing sugar and Vc content improve 10.36 ± 1.32% and 25.36 ± 2.20% respectively.
Straw organic fertilizer 3 is manured into soil and can impels rape and Chinese cabbage chlorophyll content can maintain higher level, compares with contrast 1, and growth rape in mid-term and Chinese cabbage chlorophyll content improve 26.00 ± 2.40% and 11.28 ± 1.68% respectively; Straw organic fertilizer 3 is manured into soil and can promotes rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, and compares with contrast 2, and rape and Chinese cabbage output improve 21.07 ± 2.34% and 1.80 ± 1.03% respectively; Straw organic fertilizer 3 is manured into soil and contrasts 2 and compare, and rape reducing sugar and Vc content improve 19.8 ± 3.85% and 7.69 ± 2.52% respectively; Chinese cabbage reducing sugar and Vc content improve 8.91 ± 1.58% and 20.53 ± 4.59% respectively.
Claims (8)
1. degrading straw prepares the method for fertilizer, is that the bacteria fermentation stalk with degrading straw obtains fertilizer; The active ingredient of the microbial inoculum of said degrading straw is viride (Trichoderma viride), yellow green trichoderma (Trichoderma aureoviride) and feed series bacillus (Paenibacillus pabuli);
Said viride (Trichoderma viride) is viride (Trichoderma viride) ACCC30166; Said yellow green trichoderma (Trichoderma aureoviride) is yellow green trichoderma (Trichoderma aureoviride) ACCC 32248, and said feed series bacillus (Paenibacillus pabuli) is feed series bacillus (Paenibacillus pabuli) ACCC 10273;
In the microbial inoculum of said degrading straw, the colony forming unit number of said viride (Trichoderma viride), said yellow green trichoderma (Trichoderma aureoviride) and said feed series bacillus (Paenibacillus pabuli) is than being (0.8-1.2): (0.8-1.2): (0.8-1.2).
2. method according to claim 1; It is characterized in that: in the microbial inoculum of said degrading straw, said viride (Trichoderma viride) ACCC 30166, said yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 and said feed series bacillus (Paenibacillus pabuli) ACCC 10273 independent packagings respectively.
3. method according to claim 2; It is characterized in that: in the said method; Said yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 and said feed series bacillus (Paenibacillus pabuli) ACCC 10273 insert in the stalk; Ferment after 1-2 days, insert said viride (Trichoderma viride) ACCC 30166 again, fermented again 20-30 days.
4. method according to claim 3 is characterized in that: the access amount of said yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 is 1 * 10
4-1 * 10
5Spore count/kg stalk; The access amount of said feed series bacillus (Paenibacillus pabuli) ACCC 10273 is 1 * 10
4-l * 10
5Spore count/kg stalk; The access amount of said viride (Trichoderma viride) ACCC 30166 is 1 * 10
4-1 * 10
5Spore count/kg stalk.
5. method according to claim 1; It is characterized in that: in the microbial inoculum of said degrading straw, said viride (Trichoderma viride) ACCC 30166, said yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 mix with said feed series bacillus (Paenibacillus pabuli) ACCC 10273.
6. method according to claim 5 is characterized in that: in the said method, the microbial inoculum of said degrading straw inserts in the stalk, ferments 20-30 days.
7. method according to claim 6 is characterized in that: the microbial inoculum access amount of said degrading straw is 1 * 10
4-1 * 10
5Yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 spores/kg stalk.
8. the fertilizer for preparing by arbitrary described method in the claim 1 to 7.
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| CN102718603B (en) * | 2012-07-11 | 2013-10-30 | 德州市元和农业科技开发有限责任公司 | Compound microbial fertilizer and preparation method thereof |
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| CN103288559B (en) * | 2012-11-08 | 2014-05-14 | 山东省农业科学院农产品研究所 | Biological composite bacterial fertilizer special for salvia miltiorrhiza |
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| CN105941487A (en) * | 2016-05-18 | 2016-09-21 | 杭州富阳飞博科技有限公司 | Biological weeding preparation prepared from microbes |
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