CN107828657A - A kind of preparation method of microbial bacterial agent for compost fermentation deodorization - Google Patents
A kind of preparation method of microbial bacterial agent for compost fermentation deodorization Download PDFInfo
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- CN107828657A CN107828657A CN201710958890.0A CN201710958890A CN107828657A CN 107828657 A CN107828657 A CN 107828657A CN 201710958890 A CN201710958890 A CN 201710958890A CN 107828657 A CN107828657 A CN 107828657A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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Abstract
The invention discloses a kind of preparation method of the microbial bacterial agent for compost fermentation deodorization, in order to obtain the candidate's bacterial of deodorant microbial agent, separated in being defecated from fresh fowl with to ammonia and hydrogen sulfide efficient degrading bacterial strain, then bacterial strain is fixed using mushroom bran, the method for preparing microbial bacterial agent is simple, obtained microbial bacterial agent cost is low, the stink in fermentation process can be effectively reduced in processing Composting fermentation process, and fermentation time can also be promoted, obtained tunning can be directly applied for soil can also be used in mixed way with other fertilizer, have a wide range of application.
Description
Technical field
The present invention relates to organic fertilizer processing technique field, more particularly to a kind of microbial bacterial agent for compost fermentation deodorization
Preparation method.
Background technology
With animal husbandry to it is intensive, scale is fast-developing, produce substantial amounts of feces of livestock and poultry brought to ecological environment it is huge
Big harm, data information show, about 21.7 hundred million tons of China's feces of livestock and poultry annual emissions, and actual treatment is less than 10%, therefore,
Eco-carrying capacity obtains the approval of numerous researchers as the index of evaluation sustainable development of animal husbandry.Compost is harmless at present
Change, recycling, the effective ways of fermentation process feces of livestock and poultry, recycling can be at utmost realized while environmental pollution is mitigated
Utilize, but Traditional compost is present that functional microorganism quantity is few, and fermentation period is long, rotten amount of cure is low, odor pollution is big, fertilizer efficiency damage
Lose the problems such as serious.Caused foul gas complicated component is various in composting process, is divided into nitrogen-containing compound by chemical constituent：Such as
Ammonia, indoles and amine etc.；Sulfur-containing compound：Such as hydrogen sulfide, mercaptan and thioether class；Halogen and its derivative：Such as halogenated hydrocarbons
With chlorine class etc.；Oxygen-containing organic compound：Such as phenol, alcohols and aldehydes class；Hydro carbons：Such as alkynes, alkene and alkanes, endangering ring
While border and human and livestock health, the fertilizer efficiency and agricultural value of compost are also reduced, thus controls and reduces effluvium in composting process
The discharge of body be realize high-quality, efficient, pollution-free compost and ecological environmental protection there is an urgent need to.
The content of the invention
A kind of the defects of the object of the invention is exactly to make up prior art, there is provided microorganism for compost fermentation deodorization
The preparation method of microbial inoculum.
The present invention is achieved by the following technical solutions：
A kind of preparation method of microbial bacterial agent for compost fermentation deodorization, comprises the following steps：
（1）Weigh the fresh fowl and animal excrements of 10g to be placed in the physiological saline of 90mL sterilizings, dilute 10 with sterilized water-1、10-2、10-3、
10-4、10-5With 10-6Concentration gradient, configuration beef-protein medium, Gause I culture medium, martin substratum, will not
Fowl and animal excrement with concentration gradient measures 100 μ L and is coated on above-mentioned culture medium flat plate, is placed in 30 DEG C of constant incubator culture 5d,
Then further isolated and purified using plate dilution method；
（2）By step（1）Inoculation after isolating and purifying to ammonia selective medium and hydrogen sulfide selective culture medium,
30 DEG C, incubated 5d on 180r/min shaking table, retain the bacterial strain for making bacterium solution muddy；
（3）By step（2）The strain culturing activation of middle primary election, still further weighs fresh fowl and animal excrement, according to fowl and animal excrement quality
10% inoculation primary election activated strains, be placed in 30 DEG C of thermostatic chamber and be sealed by fermentation 30 days, wherein being filled in the fowl and animal excrement sealed
Have 2% boric acid solution for absorbing ammonia and the zinc amine complex salt absorbing liquid for absorbing hydrogen sulfide, selection absorb ammonia and hydrogen sulfide effect compared with
Strong bacterial strain；
（4）Pick out step（3）Middle decomposition ammonia and hydrogen sulfide act on stronger single strain, then by them in beef extract albumen
Peptone culture medium, Gause I culture medium, the single culture on the corresponding culture medium of martin substratum are inoculated into that to co-culture base enterprising
Row shaken cultivation is to the logarithmic growth later stage；
（5）Mushroom bran is sterilized at 121 DEG C and dried after 30min, ultraviolet disinfection 10min on superclean bench is placed in, then presses
According to the mixed bacteria in 10g mushroom brans inoculation 1mL activation later stage, it is sealed after well mixed.
A kind of preparation method of microbial bacterial agent for compost fermentation deodorization, step（2）Described in remove ammonia bacterial strain
The specific method of primary election is：10mL ammonias selective medium is taken to inject 25% ammoniacal liquor 10 after autoclaving loaded on shaking in bacterium bottle
μ L, the bacterial strain isolated and purified is inoculated with, is placed in 30 DEG C of incubated 3-7d on 180r/min shaking tables, is seeded to according to 5% inoculum concentration
Shake the sealing culture of bacterium bottle, observation bacterium solution have it is unchanged, if muddy show the ability with degraded ammonia.
A kind of preparation method of microbial bacterial agent for compost fermentation deodorization, step（2）Described in vulcanisation hydrogen bacterium
Strain primary election specific method be：30 DEG C of perseverances in 180r/min shaking tables will be placed in the inoculation isolated and purified to fluid nutrient medium
Temperature culture 3-7d, is seeded to equipped with 200mL hydrogen sulfide selective culture mediums, the bottle inwall of culture medium is put according to 5% inoculum concentration
Have equipped with the sulfuric acid solution that 12mL volumetric concentrations are 25%, seal culture after 4g iron sulfide is added into sulfuric acid solution, observe bacterium solution
There is unchanged, ability of the muddiness explanation with degraded hydrogen sulfide.
A kind of preparation method of microbial bacterial agent for compost fermentation deodorization, step（4）Described in co-cultivation base group
It is divided into：Glucose 5g/L, powdered beef 1g/L, peptone 10g/L, yeast extract 5g/L, ammonium sulfate 2g/L, potassium dihydrogen phosphate
0.3g/L, magnesium sulfate 0.6g/L, manganese sulfate 0.05g/L, sodium chloride 5g/L, pH value 7.2 ± 0.2 is adjusted, then 121 DEG C, is sterilized
15min。
It is an advantage of the invention that：The present invention is in order to obtain the candidate's bacterial of deodorant microbial agent, from fresh fowl defecates
Separate with to ammonia and hydrogen sulfide efficient degrading bacterial strain, then bacterial strain is fixed using mushroom bran, prepares microbial bacteria
The method of agent is simple, and obtained microbial bacterial agent cost is low, and hair can effectively be reduced by handling in Composting fermentation process
Stink during ferment, and fermentation time can also be promoted, obtained tunning can be directly applied for soil also can and its
He is used in mixed way fertilizer, has a wide range of application.
Embodiment
A kind of preparation method of microbial bacterial agent for compost fermentation deodorization, comprises the following steps：
（1）Weigh the fresh fowl and animal excrements of 10g to be placed in the physiological saline of 90mL sterilizings, dilute 10 with sterilized water-1、10-2、10-3、
10-4、10-5With 10-6Concentration gradient, configuration beef-protein medium, Gause I culture medium, martin substratum, will not
Fowl and animal excrement with concentration gradient measures 100 μ L and is coated on above-mentioned culture medium flat plate, is placed in 30 DEG C of constant incubator culture 5d,
Then further isolated and purified using plate dilution method；
（2）10mL ammonias selective medium is taken to inject the 25% μ L of ammoniacal liquor 10, inoculation step after autoclaving loaded on shaking in bacterium bottle
Suddenly（1）In the bacterial strain that isolates and purifies, be placed in 30 DEG C of incubated 5d on 180r/min shaking tables, be seeded to and shake according to 5% inoculum concentration
The sealing culture of bacterium bottle, the incubated 5d on 30 DEG C, 180r/min shaking table, observation bacterium solution have it is unchanged, if muddiness shows have
There is the ability of degraded ammonia, by step（1）In be placed in 180r/min shaking tables on inoculation to the fluid nutrient medium that isolates and purifies
Interior 30 DEG C of incubated 5d, it is seeded to according to 5% inoculum concentration equipped with 200mL hydrogen sulfide selective culture mediums, the bottle of culture medium
Inwall be placed with equipped with 12mL volumetric concentrations be 25% sulfuric acid solution, into sulfuric acid solution add 4g iron sulfide after 30 DEG C,
Incubated 5d sealing culture on 180r/min shaking table, observation bacterium solution have it is unchanged, if muddy explanation has degraded hydrogen sulfide
Ability；
（3）By step（2）The strain culturing activation of middle primary election, still further weighs the fresh fowl and animal excrements of 200g, according to fowl and animal excrement
The activated strains of 10% inoculation primary election of quality, are placed in 30 DEG C of thermostatic chamber and are sealed by fermentation 30 days, wherein the fowl and animal excrement sealed
In equipped with the 20mL of ammonia, 2% boric acid solution and the 20mL zinc amine complex salt absorbing liquids for absorbing hydrogen sulfide is absorbed, selection absorbs ammonia
The stronger bacterial strain with hydrogen sulfide effect；
（4）Pick out step（3）Middle decomposition ammonia and hydrogen sulfide act on stronger single strain, then by them in beef extract albumen
Peptone culture medium, Gause I culture medium, the single culture on the corresponding culture medium of martin substratum are inoculated into that to co-culture base enterprising
Row shaken cultivation is to the logarithmic growth later stage, wherein the formula for co-culturing base is：Glucose 5g/L, powdered beef 1g/L, peptone
10g/L, yeast extract 5g/L, ammonium sulfate 2g/L, potassium dihydrogen phosphate 0.3g/L, magnesium sulfate 0.6g/L, manganese sulfate 0.05g/L, chlorine
Change sodium 5g/L, adjust 7.2 ± 0.2,121 DEG C of pH value, sterilize 15min；
（5）Mushroom bran is sterilized at 121 DEG C and dried after 30min, ultraviolet disinfection 10min on superclean bench is placed in, then presses
According to the mixed bacteria in 10g mushroom brans inoculation 1mL activation later stage, it is sealed after well mixed.
Claims (4)
1. the preparation method of a kind of microbial bacterial agent for compost fermentation deodorization, it is characterised in that comprise the following steps：
（1）Weigh the fresh fowl and animal excrements of 10g to be placed in the physiological saline of 90mL sterilizings, dilute 10 with sterilized water-1、10-2、10-3、
10-4、10-5With 10-6Concentration gradient, configuration beef-protein medium, Gause I culture medium, martin substratum, will not
Fowl and animal excrement with concentration gradient measures 100 μ L and is coated on above-mentioned culture medium flat plate, is placed in 30 DEG C of constant incubator culture 5d,
Then further isolated and purified using plate dilution method；
（2）By step（1）Inoculation after isolating and purifying to ammonia selective medium and hydrogen sulfide selective culture medium,
30 DEG C, incubated 5d on 180r/min shaking table, retain the bacterial strain for making bacterium solution muddy；
（3）By step（2）The strain culturing activation of middle primary election, still further weighs fresh fowl and animal excrement, according to fowl and animal excrement quality
10% inoculation primary election activated strains, be placed in 30 DEG C of thermostatic chamber and be sealed by fermentation 30 days, wherein being filled in the fowl and animal excrement sealed
Have 2% boric acid solution for absorbing ammonia and the zinc amine complex salt absorbing liquid for absorbing hydrogen sulfide, selection absorb ammonia and hydrogen sulfide effect compared with
Strong bacterial strain；
（4）Pick out step（3）Middle decomposition ammonia and hydrogen sulfide act on stronger single strain, then by them in beef extract albumen
Peptone culture medium, Gause I culture medium, the single culture on the corresponding culture medium of martin substratum are inoculated into that to co-culture base enterprising
Row shaken cultivation is to the logarithmic growth later stage；
（5）Mushroom bran is sterilized at 121 DEG C and dried after 30min, ultraviolet disinfection 10min on superclean bench is placed in, then presses
According to the mixed bacteria in 10g mushroom brans inoculation 1mL activation later stage, it is sealed after well mixed.
2. a kind of preparation method of microbial bacterial agent for compost fermentation deodorization according to claim 1, its feature exist
In step（2）Described in be except the specific method of ammonia bacterial strain primary election：Take 10mL ammonias selective medium to be loaded on and shake bacterium bottle
In, the μ L of ammoniacal liquor 10 of injection 25%, are inoculated with the bacterial strain isolated and purified, are placed in 30 DEG C of constant temperature on 180r/min shaking tables after autoclaving
Cultivate 3-7d, be seeded to according to 5% inoculum concentration shake bacterium bottle sealing culture, observation bacterium solution have it is unchanged, if muddiness show have drop
Solve the ability of ammonia.
3. a kind of preparation method of microbial bacterial agent for compost fermentation deodorization according to claim 1, its feature exist
In step（2）Described in the specific method of vulcanisation hydrogen bacterial strain primary election be：By the inoculation isolated and purified to Liquid Culture
30 DEG C of incubated 3-7d in 180r/min shaking tables are placed on base, is seeded to according to 5% inoculum concentration and is selected equipped with 200mL hydrogen sulfide
In selecting property culture medium, the bottle inwall of culture medium is placed with equipped with the sulfuric acid solution that 12mL volumetric concentrations are 25%, is added into sulfuric acid solution
Culture is sealed after entering 4g iron sulfide, observation bacterium solution has unchanged, ability of the muddiness explanation with degraded hydrogen sulfide.
4. a kind of preparation method of microbial bacterial agent for compost fermentation deodorization according to claim 1, its feature exist
In step（4）Described in co-cultivation base component be：Glucose 5g/L, powdered beef 1g/L, peptone 10g/L, yeast extract
5g/L, ammonium sulfate 2g/L, potassium dihydrogen phosphate 0.3g/L, magnesium sulfate 0.6g/L, manganese sulfate 0.05g/L, sodium chloride 5g/L, adjust pH
Value 7.2 ± 0.2, then 121 DEG C, sterilize 15min.
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| CN201710958890.0A CN107828657A (en) | 2017-10-16 | 2017-10-16 | A kind of preparation method of microbial bacterial agent for compost fermentation deodorization |
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| CN201710958890.0A CN107828657A (en) | 2017-10-16 | 2017-10-16 | A kind of preparation method of microbial bacterial agent for compost fermentation deodorization |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108585400A (en) * | 2018-04-20 | 2018-09-28 | 黄智慧 | A kind of preparation method of farm house livestock culture deodorant |
| CN108587999A (en) * | 2018-04-01 | 2018-09-28 | 魏宝阳 | A kind of screening and breeding method for lavatory and refuse depot deodorization bacterial strain |
| CN108925755A (en) * | 2018-03-26 | 2018-12-04 | 四川芬尼格斯科技有限公司 | A kind of breeding bait formula of black soldier flies larva and preparation method thereof |
| CN114703076A (en) * | 2022-03-11 | 2022-07-05 | 天津市农业科学院 | Desulfurization deamination bacterium, deodorant prepared from same and application of deodorant |
-
2017
- 2017-10-16 CN CN201710958890.0A patent/CN107828657A/en active Pending
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| 刘冬华等: "菌糠用作菌剂吸附剂的初步探索", 《中国食用菌》 * |
| 简保权等: "猪粪堆肥过程中NH3和H2S的释放及除臭微生物的筛选研究", 《农业工程学报》 * |
| 陈丽园等: "畜禽粪便除臭微生物的分离与筛选", 《畜牧与兽医》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108925755A (en) * | 2018-03-26 | 2018-12-04 | 四川芬尼格斯科技有限公司 | A kind of breeding bait formula of black soldier flies larva and preparation method thereof |
| CN108587999A (en) * | 2018-04-01 | 2018-09-28 | 魏宝阳 | A kind of screening and breeding method for lavatory and refuse depot deodorization bacterial strain |
| CN108585400A (en) * | 2018-04-20 | 2018-09-28 | 黄智慧 | A kind of preparation method of farm house livestock culture deodorant |
| CN108585400B (en) * | 2018-04-20 | 2021-12-07 | 深圳市节能协会 | Preparation method of deodorant for farming and livestock breeding in farmhouse |
| CN114703076A (en) * | 2022-03-11 | 2022-07-05 | 天津市农业科学院 | Desulfurization deamination bacterium, deodorant prepared from same and application of deodorant |
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