CN103041423B - Water lettuce deodorant - Google Patents
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- CN103041423B CN103041423B CN201210575155.9A CN201210575155A CN103041423B CN 103041423 B CN103041423 B CN 103041423B CN 201210575155 A CN201210575155 A CN 201210575155A CN 103041423 B CN103041423 B CN 103041423B
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Abstract
The invention provides a water lettuce deodorant. The water lettuce deodorant is prepared according to the following steps: inoculating lactic acid bacteria, nitrifying bacteria and bacillus according to the bacterial quantity ratio of 2:1:1 into a thallus culture medium; performing oscillating composite culture; preparing primary deodorant bacteria stoste; perform a series of operation to obtain the final deodorant bacteria stoste; and preparing the water lettuce deodorant through water lettuce liquid obtained after pretreatment on the final deodorant bacteria stoste and the water lettuce. The water lettuce deodorant has the advantage of effectively reducing the ammonia content and the hydrogen sulfide content of air to obviously eliminate the odor of a farm, and also has the characteristics of simple preparation process and low cost.
Description
[technical field]
The present invention relates to a kind of product of eliminating stink, relate in particular to a kind of water lettuce deodorizer.
[background technology]
Odorant is of a great variety, different osmophore in dissimilar material molecular structure, thereby have different abnormal smells from the patients and threshold values.In odorant, to the larger thio-alcohol of human health damage, ammonia, hydrogen sulfide, dimethyl disulfide, trimethylamine, formaldehyde, styrene, chromic acid and phenols etc., and the odorant that existing plant produces mainly contains ammonia, hydrogen sulfide.Respiratory system, blood circulation, digestive system, hormonal system and the nervous system of odor pollution main hazard human body, had a strong impact on people's the mental status, reduced quality of life.
Raising along with China's living standards of the people, the discharge of stench constantly increases the weight of for the pollution of environment, the foul smell producing constantly aggravates the pollution of environment, people's health is caused to great threat, odor pollution more and more causes people's attention, therefore the focus that material (the being deodorizer) exploitation that, elimination stink is used and use have become research.
Deodorizer is mainly divided into physical deodorizer, chemical type deodorizer, microbiocidal deodorizer etc.Physical deodorizer is to carry out deodorization by physical method, does not change the result of odor components, only changes the local concentration of foul smell, or perhaps relative concentration; Thereby chemical type deodorizer is to utilize oxidation, reduction decomposition, neutralization reaction, additive reaction, condensation reaction, ion-exchange reactions etc. that the odorant of generation is become to odorless material to destroy foul odors; Microbiocidal deodorizer is to utilize microorganism that the odorant that is dissolved in the water is absorbed in to microorganism in health, and the metabolic activity by microorganism makes its degraded.
Wherein, microbiocidal deodorizer, due to its advantage with environmental protection, is more and more subject to people's attention.But existing microbiocidal deodorizer production technology is comparatively complicated, compound microecological microbial inoculum is difficult for obtaining, the poor effect of degraded ammonia and hydrogen sulfide, and these have all limited the further application of microbiocidal deodorizer.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of water lettuce deodorizer, and it not only reduces the content of Ammonia in Air and hydrogen sulfide in efficiency ground, and also has advantages of that preparation technology is comparatively simple, cost is low.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of water lettuce deodorizer, and the concrete steps of its preparation method are as follows:
(1) prepare compound bacteria stock solution: lactobacillus, nitrifier, bacillus cereus are seeded in yeast culture base by the bacterium amount ratio of 2:1:1, and at 28 ℃ shaking table shaken cultivation 8 hours, and shaking speed 180r/min; Static cultivation 48 hours under room temperature after shaken cultivation finishes, in the process of static cultivation, get at set intervals appropriate culture fluid and measure its compound total viable count, when the compound total viable count of measuring reaches 100,000,000/milliliter, static cultivation finishes, and obtains required compound bacteria stock solution;
(2) prepare elementary deodorization bacterium stock solution: get the sewage in plant's methane-generating pit exit, with the pure water dilution of 25 times of volumes, and add the yeast culture base of 10 times of volumes, fully shake up to obtain mixed liquor; Get this mixed liquor of 10ml to clean test tube, the compound bacteria stock solution that adds 1ml step (1) gained, shake up 3 minutes and be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid odorless and pH value and is 4～5, finish constant temperature culture, and liquid is put to room temperature more than 72 hours, and make elementary deodorization bacterium stock solution, standby;
(3) the whole level of preparation deodorization bacterium stock solution: get the sewage in plant's methane-generating pit exit, standby; Get described sewage and yeast culture base and be mixed to form 1 grade of culture medium mixed liquor by the weight ratio of 1:99, in this 1 grade of culture medium mixed liquor, add the prepared elementary deodorization bacterium stock solution of step (2), and this elementary deodorization bacterium stock solution adding and the weight ratio of this 1 grade of culture medium mixed liquor are 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 1 grade of deodorization bacterium stock solution; Get afterwards described sewage and yeast culture base and be mixed to form 2 grades of culture medium mixed liquors by the weight ratio of 2:98, the 1 grade of deodorization bacterium stock solution that adds gained in these 2 grades of culture medium mixed liquors, and the weight ratio of 1 grade of deodorization bacterium stock solution of this adding and these 2 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 2 grades of deodorization bacterium stock solutions; Then get described sewage and yeast culture base and be mixed to form 3 grades of culture medium mixed liquors by the weight ratio of 4:96,2 grades of deodorization bacterium stock solutions that add gained in these 3 grades of culture medium mixed liquors, and the weight ratio of 2 grades of deodorization bacterium stock solutions of this adding and these 3 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 3 grades of deodorization bacterium stock solutions; Then get described sewage and yeast culture base and be mixed to form 4 grades of culture medium mixed liquors by the weight ratio of 6:94,3 grades of deodorization bacterium stock solutions that add gained in these 4 grades of culture medium mixed liquors, and the weight ratio of 3 grades of deodorization bacterium stock solutions of this adding and these 4 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 4 grades of deodorization bacterium stock solutions; Get described sewage and yeast culture base and be mixed to form 5 grades of culture medium mixed liquors by the weight ratio of 10:90,4 grades of deodorization bacterium stock solutions that add gained in these 5 grades of culture medium mixed liquors, and the weight ratio of 4 grades of deodorization bacterium stock solutions of this adding and these 5 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtaining 5 grades of deodorization bacterium stock solutions; Finally getting described 5 grades of culture medium mixed liquors mixes by the weight ratio of 95:5 with 5 grades of deodorization bacterium stock solutions, mix airtight constant temperature culture at rearmounted 28 ℃, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid color is yellowish and during sour in the mouth alcohol, obtain level deodorization bacterium stock solution eventually, standby;
(4) pretreatment of water lettuce: fresh water lettuce is picked up after cleaning, carry out spin-drying operation until water lettuce surface nothing is dripped naturally with the centrifuge that rotating speed is 240 turn/min; Adopt afterwards roughage mill water lettuce to be ground into the fragment being of a size of below 1.5 * 1.5cm, obtain water lettuce plant liquid;
(5) acquisition of deodorizer: get the water lettuce plant liquid that solid medium and step (4) obtain and be placed in wet mixer by 1:3 weight ratio, the deodorization of the level eventually bacterium stock solution that adds again step (3) gained, and this ultimate deodorization bacterium stock solution weight adding be in wet mixer liquid gross weight 5%, stir, put in hermetically sealed can and in 28 ℃ of bottom fermentations, after 48 hours, observe the process of fermentation, when fermented product body colour micro-brown, sour in the mouth is rotten, moisture content below 35% and clump count reach 100,000,000 CFU/ milliliters when above, obtain described water lettuce deodorizer.
Further, water lettuce deodorizer of the present invention has also carried out following operation: described water lettuce deodorizer is placed in to 55 ℃ of following temperature conditions lower lock blocks, and packs by composite aluminium film.
Further, the component of described yeast culture base is: ammonium chloride 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, zinc sulfate 0.015%, ferrous sulfate 0.025%, yeast extract 0.02%, peptone 0.01%, brown sugar 5%, and surplus is pure water; Percentage ratio in basis set minute of this yeast culture is all weight percentage.
Further, the component of described solid medium is: TONGKANG 75%, Semen Maydis powder 20%, bean cake 3%, Sal 0.5%, white sugar 0.5%, carbamide 0.5% and compound microelement potash fertilizer 0.5%; Percentage ratio in this solid medium component is all weight percentage.
Further, the method for described mensuration bacterium number is to adopt ascites method.
The beneficial effect of a kind of water lettuce deodorizer of the present invention is: this water lettuce deodorizer not only reduces to efficiency the content of Ammonia in Air and hydrogen sulfide, to play the effect of eliminating significantly stink in plant (as pig farm), and this water lettuce deodorizer also has advantages of that preparation technology is comparatively simple, cost is low, thereby water lettuce deodorizer of the present invention can be applied comparatively widely.
[specific embodiment]
A kind of water lettuce deodorizer of the present invention, the concrete steps of its preparation method are as follows:
(1) prepare compound bacteria stock solution: lactobacillus, nitrifier, bacillus cereus are seeded in yeast culture base by the bacterium amount ratio of 2:1:1, and at 28 ℃ shaking table shaken cultivation 8 hours, and shaking speed 180r/min; Static cultivation 48 hours under room temperature after shaken cultivation finishes, in the process of static cultivation, get at set intervals appropriate culture fluid and measure its compound total viable count, when the compound total viable count of measuring reaches 100,000,000/milliliter, static cultivation finishes, and obtains required compound bacteria stock solution;
(2) prepare elementary deodorization bacterium stock solution: get the sewage in plant's methane-generating pit exit, with the pure water dilution of 25 times of volumes, and add the yeast culture base of 10 times of volumes, fully shake up to obtain mixed liquor; Get this mixed liquor of 10ml to clean test tube, the compound bacteria stock solution that adds 1ml step (1) gained, shake up 3 minutes and be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid odorless and pH value and is 4～5, finish constant temperature culture, and liquid is put to room temperature more than 72 hours, and make elementary deodorization bacterium stock solution, standby;
(3) the whole level of preparation deodorization bacterium stock solution: get the sewage in plant's methane-generating pit exit, standby; Get described sewage and yeast culture base and be mixed to form 1 grade of culture medium mixed liquor by the weight ratio of 1:99, in this 1 grade of culture medium mixed liquor, add the prepared elementary deodorization bacterium stock solution of step (2), and this elementary deodorization bacterium stock solution adding and the weight ratio of this 1 grade of culture medium mixed liquor are 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 1 grade of deodorization bacterium stock solution; Get afterwards described sewage and yeast culture base and be mixed to form 2 grades of culture medium mixed liquors by the weight ratio of 2:98, the 1 grade of deodorization bacterium stock solution that adds gained in these 2 grades of culture medium mixed liquors, and the weight ratio of 1 grade of deodorization bacterium stock solution of this adding and these 2 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 2 grades of deodorization bacterium stock solutions; Then get described sewage and yeast culture base and be mixed to form 3 grades of culture medium mixed liquors by the weight ratio of 4:96,2 grades of deodorization bacterium stock solutions that add gained in these 3 grades of culture medium mixed liquors, and the weight ratio of 2 grades of deodorization bacterium stock solutions of this adding and these 3 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 3 grades of deodorization bacterium stock solutions; Then get described sewage and yeast culture base and be mixed to form 4 grades of culture medium mixed liquors by the weight ratio of 6:94,3 grades of deodorization bacterium stock solutions that add gained in these 4 grades of culture medium mixed liquors, and the weight ratio of 3 grades of deodorization bacterium stock solutions of this adding and these 4 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 4 grades of deodorization bacterium stock solutions; Get described sewage and yeast culture base and be mixed to form 5 grades of culture medium mixed liquors by the weight ratio of 10:90,4 grades of deodorization bacterium stock solutions that add gained in these 5 grades of culture medium mixed liquors, and the weight ratio of 4 grades of deodorization bacterium stock solutions of this adding and these 5 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtaining 5 grades of deodorization bacterium stock solutions; Finally getting described 5 grades of culture medium mixed liquors mixes by the weight ratio of 95:5 with 5 grades of deodorization bacterium stock solutions, mix airtight constant temperature culture at rearmounted 28 ℃, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid color is yellowish and during sour in the mouth alcohol, obtain level deodorization bacterium stock solution eventually, standby;
(4) pretreatment of water lettuce: fresh water lettuce is picked up after cleaning, with the centrifuge that rotating speed is 240 turn/min, carry out spin-drying operation until water lettuce surface (it should be noted that without naturally dripping, when water lettuce surface nothing is dripped naturally, now the overall moisture content of water lettuce is generally below 90%, and spin-drying operation reaches more than 5 minutes); Adopt afterwards roughage mill water lettuce to be ground into the fragment being of a size of below 1.5 * 1.5cm, to obtain water lettuce plant liquid;
(5) acquisition of deodorizer: get the water lettuce plant liquid that solid medium and step (4) obtain and be placed in wet mixer by 1:3 weight ratio, the deodorization of the level eventually bacterium stock solution that adds again step (3) gained, and this ultimate deodorization bacterium stock solution weight adding be in wet mixer liquid gross weight 5%, stir, put in hermetically sealed can and in 28 ℃ of bottom fermentations, after 48 hours, observe the process of fermentation, when fermented product body colour micro-brown, sour in the mouth is rotten, moisture content 30% and clump count reach 100,000,000 CFU/ milliliters when above, obtains described water lettuce deodorizer.
Conventionally water lettuce deodorizer of the present invention is placed in to 55 ℃ of following temperature conditions lower lock blocks by described water lettuce deodorizer, and is used in sale after packing by composite aluminium film.
Wherein, the component of the yeast culture base in above steps is: ammonium chloride 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, zinc sulfate 0.015%, ferrous sulfate 0.025%, yeast extract 0.02%, peptone 0.01%, brown sugar 5%, and surplus is pure water; The component of step (5) solid medium is: TONGKANG 75%, Semen Maydis powder 20%, bean cake 3%, Sal 0.5%, white sugar 0.5%, carbamide 0.5% and compound microelement potash fertilizer 0.5%; And the percentage ratio in each nutrient media components is all weight percentage; In addition, in the present invention, measure and when bacterium is counted, all adopt comparatively conventional ascites method.
Water lettuce deodorizer of the present invention is for eliminating the stink of plant, and applicant be take this compound microecological microbial inoculum and is applied to pig farm and sets forth explanation as example, particularly:
By the above-mentioned concentrated place of the even dispenser of water lettuce deodorizer fecaluria in pig farm preparing, 2～10 grams of every square metre of dispensers are not etc. as the case may be, the concentration of ammonia and hydrogen sulfide in mensurated gas composition after 5 minutes (being air), and the concentration of ammonia in gas before dispenser water lettuce deodorizer and hydrogen sulfide is measured; Wherein, CD-1 type portable type air sampler for gas sampling, and in the column home on pig farm 5 point samplings, average, repeat to test 5 times, experimental result is as following table 1.
Table 1: the degradation effect of ammonia and hydrogen sulfide
And as shown in Table 1, water lettuce deodorizer of the present invention can make ammonia, hydrogen sulfide degrade respectively 71.4% and 81.2%, and in pig farm, Ammonia in Air and hydrogen sulfide on average drop to respectively 0.173mg/m
3and 0.041mg/m
3.
To sum up, the water lettuce deodorizer that the present invention makes can reduce the content of Ammonia in Air and hydrogen sulfide effectively, play the effect of eliminating significantly stink in plant (as pig farm), and this water lettuce deodorizer also has advantages of that preparation production technology is comparatively simple, cost is low, thereby water lettuce deodorizer of the present invention can be applied comparatively widely.
Claims (5)
1. a water lettuce deodorizer, is characterized in that: the concrete steps of its preparation method are as follows:
(1) prepare compound bacteria stock solution: lactobacillus, nitrifier, bacillus cereus are seeded in yeast culture base by the bacterium amount ratio of 2:1:1, and at 28 ℃ shaking table shaken cultivation 8 hours, and shaking speed 180r/min; Static cultivation 48 hours under room temperature after shaken cultivation finishes, in the process of static cultivation, get at set intervals appropriate culture fluid and measure its compound total viable count, when the compound total viable count of measuring reaches 100,000,000/milliliter, static cultivation finishes, and obtains required compound bacteria stock solution;
(2) prepare elementary deodorization bacterium stock solution: get the sewage in plant's methane-generating pit exit, with the pure water dilution of 25 times of volumes, and add the yeast culture base of 10 times of volumes, fully shake up to obtain mixed liquor; Get this mixed liquor of 10ml to clean test tube, the compound bacteria stock solution that adds 1ml step (1) gained, shake up 3 minutes and be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid odorless and pH value and is 4～5, finish constant temperature culture, and liquid is put to room temperature more than 72 hours, and make elementary deodorization bacterium stock solution, standby;
(3) the whole level of preparation deodorization bacterium stock solution: get the sewage in plant's methane-generating pit exit, standby; Get described sewage and yeast culture base and be mixed to form 1 grade of culture medium mixed liquor by the weight ratio of 1:99, in this 1 grade of culture medium mixed liquor, add the prepared elementary deodorization bacterium stock solution of step (2), and this elementary deodorization bacterium stock solution adding and the weight ratio of this 1 grade of culture medium mixed liquor are 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 1 grade of deodorization bacterium stock solution; Get afterwards described sewage and yeast culture base and be mixed to form 2 grades of culture medium mixed liquors by the weight ratio of 2:98, the 1 grade of deodorization bacterium stock solution that adds gained in these 2 grades of culture medium mixed liquors, and the weight ratio of 1 grade of deodorization bacterium stock solution of this adding and these 2 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 2 grades of deodorization bacterium stock solutions; Then get described sewage and yeast culture base and be mixed to form 3 grades of culture medium mixed liquors by the weight ratio of 4:96,2 grades of deodorization bacterium stock solutions that add gained in these 3 grades of culture medium mixed liquors, and the weight ratio of 2 grades of deodorization bacterium stock solutions of this adding and these 3 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 3 grades of deodorization bacterium stock solutions; Then get described sewage and yeast culture base and be mixed to form 4 grades of culture medium mixed liquors by the weight ratio of 6:94,3 grades of deodorization bacterium stock solutions that add gained in these 4 grades of culture medium mixed liquors, and the weight ratio of 3 grades of deodorization bacterium stock solutions of this adding and these 4 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtain 4 grades of deodorization bacterium stock solutions; Get described sewage and yeast culture base and be mixed to form 5 grades of culture medium mixed liquors by the weight ratio of 10:90,4 grades of deodorization bacterium stock solutions that add gained in these 5 grades of culture medium mixed liquors, and the weight ratio of 4 grades of deodorization bacterium stock solutions of this adding and these 5 grades of culture medium mixed liquors is 5:95, stir and within 5 minutes, be placed on constant temperature culture at 28 ℃, after 48 hours, every 24 hours, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter, obtaining 5 grades of deodorization bacterium stock solutions; Finally getting described 5 grades of culture medium mixed liquors mixes by the weight ratio of 95:5 with 5 grades of deodorization bacterium stock solutions, mix airtight constant temperature culture at rearmounted 28 ℃, observe the process of constant temperature culture and measure its contained bacterium number, when the bacterium number of measuring reaches 100,000,000/milliliter and liquid color is yellowish and during sour in the mouth alcohol, obtain level deodorization bacterium stock solution eventually, standby;
(4) pretreatment of water lettuce: fresh water lettuce is picked up after cleaning, carry out spin-drying operation until water lettuce surface nothing is dripped naturally with the centrifuge that rotating speed is 240 turn/min; Adopt afterwards roughage mill water lettuce to be ground into the fragment being of a size of below 1.5 * 1.5cm, obtain water lettuce plant liquid;
(5) acquisition of deodorizer: get the water lettuce plant liquid that solid medium and step (4) obtain and be placed in wet mixer by 1:3 weight ratio, the deodorization of the level eventually bacterium stock solution that adds again step (3) gained, and this ultimate deodorization bacterium stock solution weight adding be in wet mixer liquid gross weight 5%, stir, put in hermetically sealed can and in 28 ℃ of bottom fermentations, after 48 hours, observe the process of fermentation, when fermented product body colour micro-brown, sour in the mouth is rotten, moisture content below 35% and clump count reach 100,000,000 CFU/ milliliters when above, obtain described water lettuce deodorizer.
2. a kind of water lettuce deodorizer according to claim 1, is characterized in that: its preparation method also comprise following operation: described water lettuce deodorizer is placed in to 55 ℃ of following temperature conditions lower lock blocks, and packs by composite aluminium film.
3. a kind of water lettuce deodorizer according to claim 1, it is characterized in that: the component of described yeast culture base is: ammonium chloride 0.1%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, zinc sulfate 0.015%, ferrous sulfate 0.025%, yeast extract 0.02%, peptone 0.01%, brown sugar 5%, and surplus is pure water; Percentage ratio in basis set minute of this yeast culture is all weight percentage.
4. a kind of water lettuce deodorizer according to claim 1, is characterized in that: the component of described solid medium is: TONGKANG 75%, Semen Maydis powder 20%, bean cake 3%, Sal 0.5%, white sugar 0.5%, carbamide 0.5% and compound microelement potash fertilizer 0.5%; Percentage ratio in this solid medium component is all weight percentage.
5. a kind of water lettuce deodorizer according to claim 1, is characterized in that: the method for described mensuration bacterium number is to adopt ascites method.
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| CN104548175B (en) * | 2014-12-31 | 2019-06-21 | 北京沃土天地生物科技有限公司 | A kind of composite biological deodorant and the preparation method and application thereof |
| CN107261818A (en) * | 2017-07-25 | 2017-10-20 | 哈尔滨天储生物科技有限公司 | A kind of liquid fowl and animal excrement deodorization fly eradication liquid and preparation method |
| CN107583432A (en) * | 2017-09-15 | 2018-01-16 | 安徽中弘科技发展有限公司 | A kind of Chinese medicine is except formaldehyde agent prescription and preparation method thereof |
| CN109126447A (en) * | 2018-09-19 | 2019-01-04 | 浙江瀚邦环保科技有限公司 | A kind of lyosoption and its application method handling low-concentration organic exhaust gas |
| CN110585879A (en) * | 2019-09-23 | 2019-12-20 | 华川技术有限公司 | Pure natural water hyacinth deodorant liquid and preparation method thereof |
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| CN101015704A (en) * | 2007-02-28 | 2007-08-15 | 袁祥生 | Method for eliminating indoor decoration pollution by using aquatic plants |
| CN102475897A (en) * | 2010-11-19 | 2012-05-30 | 江苏邦德生物科技发展有限公司 | Biological antibacterial deodorant |
| CN102813948A (en) * | 2011-06-07 | 2012-12-12 | 山西昌鑫生物农业科技有限公司 | Preparation method of composite biological deodorant |
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|---|---|---|---|---|
| JPH03280956A (en) * | 1990-03-29 | 1991-12-11 | Mitsubishi Materials Corp | Microorganismic sublimable deodorant |
| KR100847905B1 (en) * | 2007-05-19 | 2008-07-23 | 백도현 | Deodorizer of domestic animal |
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|---|---|---|---|---|
| CN101015704A (en) * | 2007-02-28 | 2007-08-15 | 袁祥生 | Method for eliminating indoor decoration pollution by using aquatic plants |
| CN102475897A (en) * | 2010-11-19 | 2012-05-30 | 江苏邦德生物科技发展有限公司 | Biological antibacterial deodorant |
| CN102813948A (en) * | 2011-06-07 | 2012-12-12 | 山西昌鑫生物农业科技有限公司 | Preparation method of composite biological deodorant |
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| Title |
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