CN108611301A - A kind of composite biological deodorant and preparation method - Google Patents
A kind of composite biological deodorant and preparation method Download PDFInfo
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- CN108611301A CN108611301A CN201810453403.XA CN201810453403A CN108611301A CN 108611301 A CN108611301 A CN 108611301A CN 201810453403 A CN201810453403 A CN 201810453403A CN 108611301 A CN108611301 A CN 108611301A
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- 239000002131 composite material Substances 0.000 title claims abstract description 50
- 239000002781 deodorant agent Substances 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 75
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 29
- 238000000855 fermentation Methods 0.000 claims abstract description 22
- 230000004151 fermentation Effects 0.000 claims abstract description 22
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 19
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 16
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 16
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 15
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 15
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 claims abstract description 14
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 239000001963 growth medium Substances 0.000 claims description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 230000001877 deodorizing effect Effects 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- BZDIAFGKSAYYFC-UHFFFAOYSA-N manganese;hydrate Chemical compound O.[Mn] BZDIAFGKSAYYFC-UHFFFAOYSA-N 0.000 claims description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- 150000004985 diamines Chemical class 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 235000005979 Citrus limon Nutrition 0.000 claims description 4
- 244000131522 Citrus pyriformis Species 0.000 claims description 4
- 244000025254 Cannabis sativa Species 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 229920000136 polysorbate Polymers 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 241000186660 Lactobacillus Species 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims description 2
- 230000009514 concussion Effects 0.000 claims description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- 241000726221 Gemma Species 0.000 claims 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 2
- 239000004310 lactic acid Substances 0.000 claims 2
- 235000014655 lactic acid Nutrition 0.000 claims 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 36
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 abstract description 18
- 229910021529 ammonia Inorganic materials 0.000 abstract description 18
- 229910000037 hydrogen sulfide Inorganic materials 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 10
- 239000007789 gas Substances 0.000 abstract description 10
- 230000002688 persistence Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract description 2
- 210000003608 fece Anatomy 0.000 abstract description 2
- 244000144972 livestock Species 0.000 abstract description 2
- 244000144977 poultry Species 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 230000003000 nontoxic effect Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000004332 deodorization Methods 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000007259 addition reaction Methods 0.000 description 1
- 238000004887 air purification Methods 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000006482 condensation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- -1 fixed-end forces Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000009705 sanhuang Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Environmental & Geological Engineering (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
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- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The present invention provides a kind of composite biological deodorants, including aerobic composite bacteria agent and anaerobism composite bacteria agent, the aerobic composite bacteria agent is by bacillus subtilis, S. cervisiae, Rhodopseudomonas palustris, nitrifier and nitrogen-fixing bacteria obtained by common fermentation under aerobic conditions, and the anaerobism composite bacteria agent is lactobacillus lactis and lactobacillus plantarum under anaerobic obtained by common fermentation.The composite biological deodorant of the present invention can remove a variety of foul gas that a large amount of feces of livestock and poultry that cultivation generates generate, 95% or more is up to the removal efficiency of ammonia in henhouse and hydrogen sulfide, 85% or more is up to the removal efficiency of ammonia and hydrogen sulfide in pig house feace storing pool, and effect persistence is strong, be conducive to improve breeding environment, reduce ambient contamination, and safe and non-toxic.
Description
Technical field
The present invention relates to field of environment protection, and in particular to a kind of biological deodorant and preparation method.
Background technology
With the development of society, problem of environmental pollution getting worse, wherein from industry, life and agricultural breeding
Foul gas drastically influences the quality of the production and life of people environment.Therefore, administering foul gas is particularly important.
Currently, common deodorant and deodorizing method relate generally to physics, chemistry, biology etc..
Physical deodorization agent is to carry out deodorization by physical method using the physical property of deodorant or foul smell not change
Odor components as a result, only change foul smell local concentration, that is, change relative concentration.Absorbing and deodorizing agent is common are, covers and removes
Smelly dose etc..
Chemical deodorizing agent is to utilize oxidation, reduction decomposition, neutralization reaction, addition reaction, condensation reaction, ion-exchange reactions
Deng the odorant of generation is become odorless substance to eliminate foul smell, however the product of chemical deodorizing have cause it is new uncontrollable
Environmental pollution possibility.
Natural plant deodorant is formed using natural disinfection deodorization elaboration in extraction plant.With antibacterial, sterilization
And deodouring effect, there is good decomposition removal effect to stenches such as ammonia, hydrogen sulfide, can be used for food medicine, fixed-end forces, sewage
The air purification and peculiar odor treatment of the industries such as processing.Its effect makes the spatial configuration of odor molecules change, and weakens peculiar smell point
Chemical combination key in son is easy to chemically react with the acidic buffer in other molecules and vegetation water, ultimately produces tasteless, nothing
The substance of poison, however natural plant deodorant extraction process is complicated, production cost is higher, and deodorization object is single.
Invention content
The object of the present invention is to provide a kind of composite biological deodorant and preparation methods.
To achieve the above object, the technical solution used in the present invention：A kind of composite biological deodorant, including aerobic compound
Microbial inoculum and anaerobism composite bacteria agent, the aerobic composite bacteria agent are by bacillus subtilis, S. cervisiae, the red false unit cell in marsh
Obtained by common fermentation under aerobic conditions, the anaerobism composite bacteria agent is lactobacillus lactis and plant for bacterium, nitrifier and nitrogen-fixing bacteria
Lactobacillus is under anaerobic obtained by common fermentation.
Preferably, the bacillus subtilis, S. cervisiae, Rhodopseudomonas palustris, nitrifier, nitrogen-fixing bacteria are aerobic
Under the conditions of spread cultivation respectively through overactivation before common fermentation, the lactobacillus lactis and lactobacillus plantarum are under anaerobic before common fermentation
It spreads cultivation respectively through overactivation.
Preferably, the ratio of aerobic composite bacteria agent and anaerobism composite bacteria agent is 1:1-1.5.
Preferably, the weight of the bacillus subtilis, S. cervisiae, Rhodopseudomonas palustris, nitrifier, nitrogen-fixing bacteria
Ratio is 2:2:3:2:2.
Preferably, the lactobacillus lactis, lactobacillus plantarum weight ratio be 1:1.
Preferably, the composite biological deodorant is by adding acid for adjusting pH to be 2.5-3.0.
It is furthermore preferred that bacillus subtilis living bacteria count >=1,000,000,000/ml, S. cervisiae have in composite biological deodorant
Imitate viable count >=1,000,000,000/ml, Rhodopseudomonas palustris living bacteria count >=1,000,000,000/ml, lactobacillus lactis living bacteria count >=1
Hundred million/ml, lactobacillus plantarum living bacteria count >=100,000,000/ml, nitrogen-fixing bacteria living bacteria count >=1,000,000,000/ml, nitrifier living bacteria count
>=1 hundred million/ml.
The present invention provides a kind of preparation method of composite biological deodorant, and described method includes following steps：By withered grass bud
Spore bacillus, S. cervisiae, Rhodopseudomonas palustris, nitrifier, inoculating to the first bacterium culture medium, at 20-40 DEG C,
Concussion carries out aerobic common fermentation under conditions of abundant illumination, obtains aerobic composite bacteria agent；Lactobacillus lactis, lactobacillus plantarum are connect
Kind is to the second bacterium culture medium, under the conditions of 30-40 DEG C, stands and carries out anaerobism common fermentation, obtain anaerobism composite bacteria agent；It will culture
At aerobic composite bacteria agent and the mixing of anaerobism composite bacteria agent, acid for adjusting pH is added into mixed deodorant to 2.5-3.0, institute
The composition weight for stating the first bacterium culture medium is：10 parts of yeast powder, 20 parts of peptone, 20 parts of brown sugar, 10 parts of glucose, sodium acetate
5.0 parts, 2.0 parts of lemon acid diamine, 0.5 part of epsom salt, 0.2 part of seven water manganese sulfate, 1000 parts of distilled water；Second bacterium
The composition weight of kind of culture medium is：10.0 parts of beef extract, 5.0 parts of yeast extract, 20.0 parts of glucose, 5.0 parts of sodium acetate, citric acid
2.0 parts of diamines, 0.5 part of epsom salt, 0.2 part of seven water manganese sulfate, 1 part of Tween 80,1000 parts of distilled water, second bacterium
Kind Medium's PH Value is 6.0.
Preferably, the bacillus subtilis, S. cervisiae, Rhodopseudomonas palustris, nitrifier, nitrogen-fixing bacteria are aerobic
Under the conditions of spread cultivation respectively through overactivation before common fermentation, the lactobacillus lactis and lactobacillus plantarum are under anaerobic before common fermentation
It spreads cultivation respectively through overactivation.
Preferably, the method further includes following steps, carries out product testing, the quality requirement that product testing reaches：It is withered
Careless bacillus living bacteria count >=1,000,000,000/ml, S. cervisiae living bacteria count >=1,000,000,000/ml, Rhodopseudomonas palustris have
Effect viable count >=1,000,000,000/ml, lactobacillus lactis living bacteria count >=100,000,000/ml, lactobacillus plantarum living bacteria count >=100,000,000/ml,
Nitrogen-fixing bacteria living bacteria count >=1,000,000,000/ml, nitrifier living bacteria count >=100,000,000/ml, enzyme activity is qualified shortly, filling after qualified
Storage.
The beneficial effects of the present invention are：
The composite biological deodorant of the present invention can remove a variety of foul gas that a large amount of feces of livestock and poultry that cultivation generates generate,
95% or more is up to the removal efficiency of ammonia in henhouse and hydrogen sulfide, the removal of ammonia and hydrogen sulfide in pig house feace storing pool is imitated
Rate is up to 85% or more, and effect persistence is strong, is conducive to improve breeding environment, reduces ambient contamination, and safety
Nonhazardous.
For the object, technical solutions and advantages of the present invention are better described, below in conjunction with specific embodiment to the present invention
It further illustrates.It will be appreciated by those skilled in the art that described herein, specific examples are only used to explain the present invention, not
For limiting the present invention.
Specific implementation mode
Embodiment 1
The preparation of composite biological deodorant：
The first step：By bacillus subtilis, S. cervisiae, nitrifier, Rhodopseudomonas palustris, lactobacillus plantarum, breast
Lactobacillus lactis, nitrogen-fixing bacteria are seeded in culture tank activate and spread cultivation respectively.
Second step：It will be by 1.0% parts by weight bacillus subtilis, 1.0% parts by weight S. cervisiae, 1.5% parts by weight
The weight ratios such as the aerobic composite bacteria that Rhodopseudomonas palustris, 1.0% parts by weight nitrifier, 1.5% parts by weight nitrogen-fixing bacteria form
It is inoculated into the first bacterium culture medium, the abundant illumination shaken cultivation of composite bacteria；30 DEG C of fermentation temperature is controlled, 3-5 is carried out
It aerobic common fermentation, makes aerobic flora be proliferated rapidly as dominant microflora；It will be by 1.0% parts by weight lactobacillus lactis, 1.0%
The weight ratios such as the anaerobism composite bacteria of parts by weight lactobacillus plantarum composition are inoculated into the second bacterium culture medium, stationary culture, control
35 DEG C of fermentation temperature processed carries out 3-5 days or so anaerobism common fermentations, so that anaerobic bacteria flora is proliferated rapidly, first Spawn incubation
The composition weight of base is：10 parts of yeast powder, 20 parts of peptone, 20 parts of brown sugar, 10 parts of glucose, 5.0 parts of sodium acetate, citric acid two
2.0 parts of amine, 0.5 part of epsom salt, 0.2 part of seven water manganese sulfate, 1000 parts of distilled water；The component of second bacterium culture medium
Weight is：10.0 parts of beef extract, 5.0 parts of yeast extract, 20.0 parts of glucose, 5.0 parts of sodium acetate, 2.0 parts of lemon acid diamine, seven water
0.5 part of magnesium sulfate, 0.2 part of seven water manganese sulfate, 1 part of Tween 80,1000 parts of distilled water, the second bacterium culture medium pH value are 6.0.
Third walks：Second step is obtained into composite bacteria agent by weight proportion 1:1 mix and adds acid for adjusting pH to 3.0.
With the deodorisation process and effect of composite biological deodorant prepared by the preparation method of above-mentioned composite biological deodorant：
Inspection example 1
1, the method for inspection
Select two broiler houses, the identical Sanhuang chicken of about 8000 25 age in days health status of every henhouse, by actual production
It carries out examination to support, be carried out in such a way that ground is put down and supported.A wherein house is that control group is without any processing, and another is test group
This product, application method are added on bedding and padding：500g composite biological deodorants of the present invention are added in every 30 square meter bedding and padding, use 500g
Rice bran and 500g products of the present invention are uniformly sprinkling upon on bedding and padding after mixing well.
With Drager X-am7000 portable gas monitor every night 19 during experiment：00 measures ammonia in henhouse
With the concentration of hydrogen sulfide, the moisture of bedding and padding and temperature, sampling in continuous 30 days is recorded, and is averaged.
2, experimental result
The testing result of ammonia and concentration of hydrogen sulfide is as shown in table 1 in experimental group henhouse：Compared with the control group, of the invention
Composite biological deodorant can effectively remove ammonia and hydrogen sulfide gas in henhouse, and clearance rate is up to 95% or more.Moreover, biology removes
The persistence of smelly dose of effect is strong, applies 500g/30m in chicken farm2Amount, being averaged for ammonia concentration can be kept in 30 days
Level is 1ppm, and the average level content concn of hydrogen sulfide is monitored less than Drager X-am7000 portable gas in 30 days
The detection limit of device.
Ammonia and concentration of hydrogen sulfide testing result in 1 henhouse of table
| Group | Ammonia (ppm) | Hydrogen sulfide (ppm) | Moisture (%) | Temperature (DEG C) |
| Control group | 45 | 6 | 43 | 39 |
| Test group | 1 | 0 | 40 | 36 |
Inspection example 2
3 pig house feace storing pools similar in alternative condition, labelling experiment group 1 (other company's similar products), (this of experimental group 2
Invention product), control group, excrement is with a collection of excrement in feace storing pool.Adding method is：By 500g/m3Uniformly turned over after sprinkling
Heap.Measure ammonia and sulfureted hydrogen gas concentration in feace storing pool within every 3 days.
During experiment the dense of ammonia and hydrogen sulfide in feace storing pool is measured with Drager X-am7000 portable gas monitors
Degree.It continuous 15 days and is recorded, is averaged.
Experimental result
Ammonia and concentration of hydrogen sulfide testing result in 2 feace storing pool of table
| Group | Ammonia (ppm) | Hydrogen sulfide (ppm) |
| Control group | 65 | 18 |
| Experimental group 1 | 12 | 2 |
| Experimental group 2 | 8 | 2 |
The testing result of ammonia and concentration of hydrogen sulfide is as shown in table 2 in feace storing pool：Compared with the control group, of the invention compound
Biological deodorant can effectively remove ammonia and hydrogen sulfide gas in feace storing pool, and clearance rate is up to 85% or more.Moreover, biological deodorizing
The persistence of agent effect is strong, applies 500g/m in feace storing pool3Amount, the average level of ammonia concentration can be kept in 15 days
Less than 10ppm, it is 2ppm that the average level of concentration of hydrogen sulfide is kept in 15 days.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of range is protected, although being explained in detail to the present invention with reference to preferred embodiment, those skilled in the art should
Understand, technical scheme of the present invention can be modified or replaced equivalently, without departing from the essence of technical solution of the present invention
And range.
Claims (9)
1. a kind of composite biological deodorant, which is characterized in that described aerobic multiple comprising aerobic composite bacteria agent and anaerobism composite bacteria agent
Combined bacteria agent is total under aerobic conditions by bacillus subtilis, S. cervisiae, Rhodopseudomonas palustris, nitrifier and nitrogen-fixing bacteria
Obtained by fermentation, the anaerobism composite bacteria agent is lactobacillus lactis and lactobacillus plantarum under anaerobic obtained by common fermentation.
2. composite biological deodorant according to claim 1, which is characterized in that the bacillus subtilis, saccharomyces cerevisiae
Bacterium, Rhodopseudomonas palustris, nitrifier, nitrogen-fixing bacteria spread cultivation through overactivation respectively before common fermentation under aerobic conditions, the lactic acid
Lactobacillus and lactobacillus plantarum spread cultivation through overactivation before common fermentation respectively under anaerobic.
3. composite biological deodorant according to claim 1, which is characterized in that aerobic composite bacteria agent and anaerobism composite bacteria agent
Weight ratio be 1:1-1.5.
4. according to the composite biological deodorant described in claim 1-3 any claims, which is characterized in that the withered grass gemma
Bacillus, S. cervisiae, Rhodopseudomonas palustris, nitrifier, nitrogen-fixing bacteria weight ratio be 2:2:3:2:2.
5. according to the composite biological deodorant described in claim 1-3 any claims, which is characterized in that the lactic acid breast bar
Bacterium, lactobacillus plantarum weight ratio be 1:1.
6. according to the composite biological deodorant described in claim 1-3 any claims, which is characterized in that the biological deodorizing
The pH of agent is 2.5-3.0.
7. composite biological deodorant according to claim 6, which is characterized in that there is bacillus subtilis in biological deodorant
Imitate viable count >=1,000,000,000/ml, S. cervisiae living bacteria count >=1,000,000,000/ml, Rhodopseudomonas palustris living bacteria count >=10
Hundred million/ml, lactobacillus lactis living bacteria count >=100,000,000/ml, lactobacillus plantarum living bacteria count >=100,000,000/ml, nitrogen-fixing bacteria are effectively living
Bacterium number >=1,000,000,000/ml, nitrifier living bacteria count >=100,000,000/ml.
8. a kind of preparation method of composite biological deodorant, which is characterized in that described method includes following steps：By withered grass gemma
Bacillus, S. cervisiae, Rhodopseudomonas palustris, nitrifier and inoculating are to the first bacterium culture medium, at 20-40 DEG C,
Concussion carries out aerobic common fermentation under conditions of abundant illumination, obtains aerobic composite bacteria agent；By lactobacillus lactis and lactobacillus plantarum
It is inoculated into the second bacterium culture medium, under the conditions of 30-40 DEG C, stands and carries out anaerobism common fermentation, obtain anaerobism composite bacteria agent；It will training
Aerobic composite bacteria agent and anaerobism the composite bacteria agent mixing formed, acid for adjusting pH are added into mixed deodorant to 2.5-3.0,
The composition weight of first bacterium culture medium is：10 parts of yeast powder, 20 parts of peptone, 20 parts of brown sugar, 10 parts of glucose, acetic acid
5.0 parts of sodium, 2.0 parts of lemon acid diamine, 0.5 part of epsom salt, 0.2 part of seven water manganese sulfate, 1000 parts of distilled water；Described second
The composition weight of bacterium culture medium is：10.0 parts of beef extract, 5.0 parts of yeast extract, 20.0 parts of glucose, 5.0 parts of sodium acetate, lemon
2.0 parts of acid diamine, 0.5 part of epsom salt, 0.2 part of seven water manganese sulfate, 1 part of Tween 80,1000 parts of distilled water, described second
Bacterium culture medium pH value is 6.0.
9. the preparation method of composite biological deodorant according to claim 8, which is characterized in that the bacillus subtilis
Bacterium, S. cervisiae, Rhodopseudomonas palustris, nitrifier, nitrogen-fixing bacteria are expanded through overactivation respectively before common fermentation under aerobic conditions
Training, the lactobacillus lactis and lactobacillus plantarum spread cultivation through overactivation before common fermentation respectively under anaerobic.
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| CN110975592A (en) * | 2019-12-11 | 2020-04-10 | 保定市隆澳环境治理有限公司 | Microbial deodorant and preparation method thereof |
| DE102019201159A1 (en) | 2019-01-30 | 2020-07-30 | Skw Stickstoffwerke Piesteritz Gmbh | Highly effective concentrates with PPDA and their use to stabilize the valuable substances in animal excrement |
| CN113528387A (en) * | 2021-07-15 | 2021-10-22 | 武汉惠斯顿环保科技有限公司 | Microbial preparation, preparation method thereof and application thereof in deodorization |
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| CN102732452A (en) * | 2012-05-23 | 2012-10-17 | 绍兴虞林生物科技有限公司 | Biological deodorant |
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| CN110975592A (en) * | 2019-12-11 | 2020-04-10 | 保定市隆澳环境治理有限公司 | Microbial deodorant and preparation method thereof |
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| CN113528387B (en) * | 2021-07-15 | 2022-03-08 | 武汉惠斯顿环保科技有限公司 | Microbial preparation, preparation method thereof and application thereof in deodorization |
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