KR101881015B1 - Composition for biofloc water purification using fish farm comprising effective microorganisms as effective component and uses thereof - Google Patents
Composition for biofloc water purification using fish farm comprising effective microorganisms as effective component and uses thereof Download PDFInfo
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- KR101881015B1 KR101881015B1 KR1020160175499A KR20160175499A KR101881015B1 KR 101881015 B1 KR101881015 B1 KR 101881015B1 KR 1020160175499 A KR1020160175499 A KR 1020160175499A KR 20160175499 A KR20160175499 A KR 20160175499A KR 101881015 B1 KR101881015 B1 KR 101881015B1
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- bacillus
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 239000000203 mixture Substances 0.000 title claims abstract description 25
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 23
- 238000000746 purification Methods 0.000 title claims description 6
- 244000005700 microbiome Species 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 24
- 241000178268 Saccharomycopsis schoenii Species 0.000 claims abstract description 13
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 10
- 241000315694 Bacillus tequilensis Species 0.000 claims abstract description 10
- 239000010865 sewage Substances 0.000 claims abstract description 5
- 238000004332 deodorization Methods 0.000 claims description 17
- 241000611801 Paenibacillus illinoisensis Species 0.000 claims description 7
- 239000004480 active ingredient Substances 0.000 claims description 6
- 239000002361 compost Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 19
- 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 abstract description 8
- 230000001877 deodorizing effect Effects 0.000 abstract description 7
- 241000179039 Paenibacillus Species 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 38
- 235000019645 odor Nutrition 0.000 description 20
- 229910021529 ammonia Inorganic materials 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 6
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000286209 Phasianidae Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- WQOXQRCZOLPYPM-UHFFFAOYSA-N dimethyl disulfide Chemical compound CSSC WQOXQRCZOLPYPM-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000013379 molasses Nutrition 0.000 description 2
- 125000000896 monocarboxylic acid group Chemical group 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 description 1
- WJMFXQBNYLYADA-UHFFFAOYSA-N 1-(3,4-dihydroxyphenyl)-6,7-dihydroxy-1,2-dihydronaphthalene-2,3-dicarboxylic acid Chemical compound C12=CC(O)=C(O)C=C2C=C(C(O)=O)C(C(=O)O)C1C1=CC=C(O)C(O)=C1 WJMFXQBNYLYADA-UHFFFAOYSA-N 0.000 description 1
- 241000252087 Anguilla japonica Species 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 241000252229 Carassius auratus Species 0.000 description 1
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- QMMFVYPAHWMCMS-UHFFFAOYSA-N Dimethyl sulfide Chemical compound CSC QMMFVYPAHWMCMS-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000235004 Saccharomycopsis fibuligera Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229960000846 camphor Drugs 0.000 description 1
- 229930008380 camphor Natural products 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- QBWCMBCROVPCKQ-UHFFFAOYSA-N chlorous acid Chemical class OCl=O QBWCMBCROVPCKQ-UHFFFAOYSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011436 cob Substances 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000009372 pisciculture Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
본 발명은 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 테퀼렌시스(Bacillus tequilensis), 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주를 혼합한 혼합 균주 또는 이의 배양액을 유효성분으로 함유하는 탈취 및 어류 양식장 수질정화용 조성물, 상기 조성물을 오폐수처리장, 축사, 퇴비공장 또는 화장실에 처리하여 악취를 제거하는 방법 및 상기 조성물을 어류 양식장에 처리하여 수질을 정화시키는 방법에 관한 것이다.The present invention is Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens), Bacillus Te kwilren system (Bacillus tequilensis), Penny Bacillus IL sensor system (Paenibacillus a composition for treating water in a deodorizing and fish breeding field containing an effective ingredient of a mixed strain or a mixture of Saccharomycopsis schoenii strain of the present invention and an illinoisensis and a Saccharomycopsis schoenii strain and treating the composition in a sewage treatment plant, And a method for purifying water quality by treating the composition in a fish farm.
Description
본 발명은 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 테퀼렌시스(Bacillus tequilensis), 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주를 혼합한 혼합 균주 또는 이의 배양액을 유효성분으로 함유하는 탈취 및 어류 양식장 수질정화용 조성물, 상기 조성물을 오폐수처리장, 축사, 퇴비공장 또는 화장실에 처리하여 악취를 제거하는 방법 및 상기 조성물을 어류 양식장에 처리하여 수질을 정화시키는 방법에 관한 것이다.The present invention is Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens), Bacillus Te kwilren system (Bacillus tequilensis), Penny Bacillus IL sensor system (Paenibacillus a composition for treating water in a deodorizing and fish breeding field containing an effective ingredient of a mixed strain or a mixture of Saccharomycopsis schoenii strain of the present invention and an illinoisensis and a Saccharomycopsis schoenii strain and treating the composition in a sewage treatment plant, And a method for purifying water quality by treating the composition in a fish farm.
최근 들어 생활수준의 향상에 따라 일상생활의 불쾌감을 주는 소음, 진동 또는 먼지뿐만 아니라 악취도 공해로 규정하여 관련 법규에 의한 규제가 강화되고 있는 실정이다. 통상적으로 악취는 황화수소, 메틸머캅탄, 황화메틸 및 이황화메틸 등과 같은 산성 냄새; 암모니아, 트리메틸아민 및 스카톨 등과 같은 염기성 냄새; 또는 아세트알데히드 및 스티렌 등과 같은 중성 냄새로 구별된다. 이러한 악취를 제거하기 위한 종래의 탈취 방법은 향료 또는 향기와 같은 방향성 물질을 이용하여 후각적으로 은폐하는 의화적 탈취법 (마스킹법); 산화, 환원, 중화, 부가축합 및 이온교환반응 등을 이용하여 분해하는 화학적 탈취법; 활성탄, 제올라이트 및 실리카겔 등의 다공성 물질을 이용하여 포집하는 물리적 흡착법; 또는 미생물, 효소 및 방부제 등을 이용하여 억제하는 생물적 탈취법으로 대별된다. 하지만 의화적 탈취는 천연 또는 합성 성분에 따라서 새로운 악취나 독성을 발생하기 쉽고, 물리적 흡착법은 효과가 빠르나 환경 조건 또는 장시간 사용에 따라 악취가 재생되고, 화학적 탈취법은 다양한 발전이 가능하지만 선택성 기작에 의해 총체적인 탈취력의 발휘에 한계가 있다.In recent years, as the level of living has improved, not only noise, vibration, or dust, which give offensive discomfort to everyday life, have been stipulated as pollution. Typically the odor is an acidic odor such as hydrogen sulfide, methyl mercaptan, methyl sulfide and methyl disulfide; Basic odors such as ammonia, trimethylamine and scallol; Or neutral odor such as acetaldehyde and styrene. Conventional deodorization methods for removing such odors include a chemical deodorization method (masking method) in which a fragrance or a fragrance is used to hide the fragrance using a directional substance; Chemical deodorization method in which decomposition is carried out by oxidation, reduction, neutralization, addition condensation and ion exchange reaction; A physical adsorption method of collecting by using a porous material such as activated carbon, zeolite and silica gel; Or a biological deodorization method in which microorganisms, enzymes and preservatives are used to inhibit them. However, the chemical deodorization is likely to generate new odor or toxicity depending on the natural or synthetic component, and the physical adsorption method is effective. However, the odor is regenerated according to the environmental conditions or the prolonged use, and the chemical deodorization method can develop variously. However, There is a limit to the overall deodorization performance.
구체적으로 악취물질로는 암모니아, 메틸머캅탄, H2S, 설픽메틸, 디설픽 메틸, 트리메틸아민, CH3COOH 및 스티렌 등이 있으며, 특히 대표적인 물질은 암모니아, 아민류로서 악취의 큰 원인이다. 암모니아는 일차적으로 눈을 따갑게 할 만큼 자극적이며, 특히 호흡기 질병발생과 큰 연관성을 갖는다. 이러한 질산성 질소와 암모니아성 질소 및 다량으로 포함된 유기물 등이 주요 악취 유발요인으로 작용하여 오폐수 처리장, 축사, 가축의 분뇨처리 또는 퇴비공정 등과 같은 악취제거 처리과정에서 많은 부작용을 야기하고 있다. 또한, 만성적으로 노출될 경우, 스트레스 요인으로 작용하기 때문에 가축뿐만 아니라 사람의 건강상태에 유해한 영향을 미친다. 따라서 간단하고 효율적인 악취를 제거할 수 있는 방법을 개발하기 위한 관심이 계속되고 있다.Specific examples of the odor substances include ammonia, methyl mercaptan, H 2 S, sulfimethyl, disulfimethyl, trimethylamine, CH 3 COOH and styrene. Representative substances are ammonia and amines, which are major causes of odor. Ammonia is primarily irritating to the eye and is particularly associated with respiratory disease outbreaks. These nitrate nitrogen, ammonia nitrogen, and organic matter contained in a large amount act as main causes of the malodor, which causes many side effects in the odor removal process such as sewage treatment plant, housing, livestock manure treatment or composting process. In addition, when exposed chronicly, it acts as a stressor, which has a detrimental effect on not only livestock but also human health. Therefore, there is a continuing interest in developing a simple and efficient way to remove odors.
육상 가두리 양식장, 호수, 연못 등의 담수 저장소의 생태 환경을 이용하여 수산물을 양식, 생산하는 경우, 수질 및 저질이 오염되어 생산량이 감소하는 문제가 있다. 특히, 이러한 수질 및 저질이 오염된 환경에서 생산된 수산물은 섭취하는 소비자의 건강에 악영향을 미칠 염려가 있고, 수질 및 저질 오염으로 인하여 담수의 변색이 발생하고 환경오염의 가능성이 높아진다.When the aquatic products are produced and produced by using the ecological environment of the freshwater reservoirs such as aquaculture farms, lakes, ponds, etc., water quality and low quality are contaminated and the production amount is decreased. Particularly, the aquatic products produced in an environment contaminated with such water quality and low quality may have an adverse effect on the health of the consumer, and discoloration of fresh water occurs due to water quality and low quality contamination, and the possibility of environmental pollution increases.
종래에는 이러한 문제를 해결하기 위하여 강제 흡입식 어장 정화 방법을 통하여 바닥 표면에 형성된 퇴적물을 수거하고 있으나, 이러한 방법으로는 근본적인 수질 및 저질 개선이 이루어지기 어렵고, 시간과 비용이 많이 소요되는 문제점이 있었다.Conventionally, in order to solve such a problem, sediments formed on the floor surface are collected through a forced inhalation type fish-cleaning method. However, this method has a problem that it is difficult to improve the quality of water and low quality and requires a long time and cost.
한국등록특허 제1029346호에는 악취가스 감소 및 항균효과를 갖는 복합미생물 발효액 및 그의 사용방법이 개시되어 있고, 한국등록특허 제1187731호에는 탈취 활성 및 수질정화 기능을 갖는 락토바실러스 파라파라기니스 균주가 개시되어 있으나, 본 발명에서와 같이 유용미생물을 유효성분으로 함유하는 수산양식용 바이오플락 수질정화용 조성물 및 이의 용도에 관해서는 밝혀진 바가 없다.Korean Patent No. 1029346 discloses a complex microbial fermentation broth having reduced odor gas and antimicrobial effect and a method of using the same. Korean Patent No. 1187731 discloses a lactobacillus paraparcaginous strain having a deodorizing activity and a water purification function. However, as in the present invention, the composition for treating bioflav water for aquaculture containing useful microorganisms as an active ingredient and its use have not been disclosed.
본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명자는 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 테퀼렌시스(Bacillus tequilensis), 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주를 혼합한 혼합물을 어류 양식장에 처리한 경우, 기존의 사료 투여시 수조에서 발생하는 아질산과 암모니아 가스 발생을 현저히 감소시킴으로써, 적어도 하루에 한번 100% 환수를 필요로 하던 어류 양식 수조에서 낮은 환수율로도 수온 및 pH가 일정하게 유지되어 보다 경제적으로 어류 양식이 가능한 것을 확인함으로써, 본 발명을 완성하였다.The present invention been made by the request as described above, the present inventors Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens , Bacillus tequilensis , Paenibacillus < RTI ID = 0.0 > illinoisensis ) and Saccharomycopsis schoenii strains in fish farms significantly reduced the production of nitrite and ammonia gas in the aquarium during the feeding of the fish, which resulted in a reduction in the fish stock tank that required 100% The water temperature and the pH were kept constant even at the water exchange rate, thereby confirming that the fish culture was possible more economically, thereby completing the present invention.
상기 과제를 해결하기 위해, 본 발명은 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 테퀼렌시스(Bacillus tequilensis), 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주를 혼합한 혼합 균주 또는 이의 배양액을 유효성분으로 함유하는 탈취 및 어류 양식장 수질정화용 조성물을 제공한다.In order to solve the above problems, the present invention is Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens), Bacillus Te kwilren system (Bacillus tequilensis), Penny Bacillus IL sensor system (Paenibacillus illinoisensis ) and Saccharomycopsis schoenii ) or a culture solution thereof as an active ingredient, and to provide a composition for purifying water quality in a fish culture farm.
또한, 본 발명은 상기 조성물을 오폐수처리장, 축사, 퇴비공장 또는 화장실에 처리하여 악취를 제거하는 방법을 제공한다.The present invention also provides a method for removing odors by treating the composition in a wastewater treatment plant, a house, a compost plant, or a toilet.
또한, 본 발명은 상기 조성물을 어류 양식장에 처리하여 수질을 정화시키는 방법을 제공한다.Further, the present invention provides a method for purifying water quality by treating the above-mentioned composition to a fish farm.
본 발명의 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 테퀼렌시스(Bacillus tequilensis), 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주를 혼합한 혼합물은 악취생성의 주요 성분인 암모니아의 탈취 활성 효과가 매우 우수하므로, 상기 혼합물을 유효성분으로 함유하는 조성물을 오폐수 처리장, 축사, 퇴비공장 또는 화장실 등과 같이 악취가 많이 나는 곳에 처리하여 빠른 시간 내에 간단하게 악취를 제거할 수 있으므로 산업용뿐만 아니라 가정용으로도 매우 유용하게 사용될 수 있다.Bacillus of the present invention amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens), Bacillus Te kwilren system (Bacillus tequilensis), Penny Bacillus IL sensor system (Paenibacillus illinoisensis and Saccharomycopsis schoenii strains are highly effective in deodorizing ammonia, which is a major component of odor generation. Therefore, a composition containing the above-mentioned mixture as an active ingredient can be used as an effective ingredient in sewage treatment plants, It can be easily used not only for industrial use but also for domestic use because it can remove odor easily in a short time by treating a place having a lot of odor such as a compost factory or a toilet.
또한, 본 발명의 혼합물을 어류 양식장에 처리한 경우 수조의 최소한의 환수율로도 장기간 수온 및 pH가 일정하게 유지되므로 어류 양식장 산업의 경제성에 크게 이바지할 수 있다.In addition, when the mixture of the present invention is treated in a fish farm, the water temperature and pH are kept constant over a long period even with a minimum water exchange rate of the water tank, which can greatly contribute to the economical efficiency of the fish farming industry.
본 발명의 목적을 달성하기 위하여, 본 발명은 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 테퀼렌시스(Bacillus tequilensis), 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주를 혼합한 혼합 균주 또는 이의 배양액을 유효성분으로 함유하는 탈취 및 어류 양식장 수질정화용 조성물을 제공한다.According to an aspect of the invention, the invention is Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens), Bacillus Te kwilren system (Bacillus tequilensis), Penny Bacillus IL sensor system (Paenibacillus illinoisensis ) and Saccharomycopsis schoenii ) or a culture solution thereof as an active ingredient, and to provide a composition for purifying water quality in a fish culture farm.
본 발명의 탈취 및 어류 양식장 수질정화용 조성물에서, 상기 어류는 뱀장어일 수 있으나, 이에 제한되지 않는다.In the deodorizing and composition for water purification of a fish farm in the present invention, the fish may be eel, but is not limited thereto.
"탈취"의 의미는 악취를 제거하는 능력을 의미하며, 상기 악취를 유발하는 악취물질로는 암모니아, 메틸머캅탄, H2S, 설픽메틸(sulfic methyl), 디설픽 메틸, 트리메틸아민, CH3COOH 및 스티렌 등일 수 있으며, 바람직하게는 암모니아이나, 이에 제한되지 않는다.The term "deodorization" means the ability to remove odor. The odor substances causing the odor include ammonia, methyl mercaptan, H 2 S, sulfic methyl, disulfimethyl, trimethylamine, CH 3 COOH, styrene, and the like, preferably ammonia, but not limited thereto.
본 발명의 어류 양식장의 수질정화 기능은 어류의 분비물 또는 수조에 투입된 사료의 부패 등으로 인해 발생하는 아질산(NO2) 또는 암모니아(NH3) 가스의 제거를 의미하는 것이나, 이에 제한되지 않는다.The water purification function of the fish farm of the present invention means the removal of nitrite (NO 2 ) or ammonia (NH 3 ) gas caused by decay of fish secretion or feed into the water tank, but is not limited thereto.
또한, 본 발명은 상기 조성물을 오폐수처리장, 축사, 퇴비공장 또는 화장실에 처리하여 악취를 제거하는 방법을 제공한다.The present invention also provides a method for removing odors by treating the composition in a wastewater treatment plant, a house, a compost plant, or a toilet.
상기 축사라 함은 돼지, 소, 염소 등의 포유류, 잉어, 금붕어 등의 어류 및 꿩, 닭, 오리, 칠면조 등의 가금류를 사육하는 공간일 수 있으나, 이에 제한되지 않는다.The barn may be a space for breeding poultry such as pigs, cows, and chlorites, fish such as carp, goldfish, and poultry such as pheasants, chickens, ducks, and turkeys, but is not limited thereto.
또한, 본 발명은 상기 조성물을 어류 양식장에 처리하여 수질을 정화시키는 방법을 제공한다.Further, the present invention provides a method for purifying water quality by treating the above-mentioned composition to a fish farm.
이하, 본 발명의 실시예를 들어 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
제조예Manufacturing example 1: 혼합 균주 배양액 준비 1: Preparation of mixed culture medium
생물자원센터(KCTC)에서 분양받은 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens), 바실러스 테퀼렌시스(Bacillus tequilensis), 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주를 혼합한 혼합 균주를 배지에 접종한 후 25~35℃에서 배양하여 균주 농도가 2.6×108 CFU/ml인 배양액을 준비하였다.Biological Resource Center Bacillus amyl Lori query Pacifico Enschede (Bacillus amyloliquefaciens) received from the pre-sale (KCTC), Bacillus Te kwilren system (Bacillus Tequilensis , Paenibacillus illinoisensis , and Saccharomycopsis schoenii strains were inoculated into the culture medium and cultured at 25 to 35 ° C. to prepare a culture solution having a strain concentration of 2.6 × 10 8 CFU / ml.
상기 배지는 녹차 잎 및 광나무 잎이 7:3의 중량비로 포함된 탄소원 혼합물 6 L에 이온화된 미네랄 2 중량%, 천연과즙 10 중량%, 천연식초 3 중량%, 소금 5 중량%, 감미성분 3 중량% 및 당밀 77 중량%가 첨가된 미네랄 혼합물 3 L를 첨가하여 제조된 pH 4.8~5의 혼합 배지를 25℃에서 45일간 발효시킨 혼합 배지에 고온멸균시킨 2~3%(w/v) 당밀 희석액을 0.6%(v/v)의 비율이 되도록 첨가한 후, pH 3.2~3.7로 적정한 배지이다.The medium consisted of 2% by weight of ionized minerals, 10% by weight of natural juice, 3% by weight of natural vinegar, 5% by weight of salt, 3% by weight of sweet potato components in 6 liters of carbon source mixture containing green tea leaves and camphor leaves at a weight ratio of 7: (W / v) molasses diluted with high-temperature sterilized mixed media prepared by adding 3 L of a mineral mixture with a pH of 4.8 to 5 and molasses molarity of 77 to 5% To a concentration of 0.6% (v / v), and then the pH is adjusted to 3.2 to 3.7.
비교예Comparative Example 1: 혼합 균주 배양액 준비 1: Preparation of mixed culture medium
상기 제조예 1의 방법으로 혼합 균주 배양액을 준비하되, 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 및 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주는 혼합하지 않고, 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens) 및 바실러스 테퀼렌시스(Bacillus tequilensis)를 이용하여 2.6×108 CFU/ml 농도의 혼합 균주 배양액을 준비하였다.A culture solution of the mixed strain was prepared by the method of Preparation Example 1, except that Paenibacillus illinoisensis ) and Saccharomycopsis schoenii) strains without mixing, Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens ) and Bacillus tequilensis were used to prepare a culture solution of the mixed strain at a concentration of 2.6 x 10 8 CFU / ml.
비교예Comparative Example 2: 혼합 균주 배양액 준비 2: preparation of mixed culture medium
상기 제조예 1의 방법으로 혼합 균주 배양액을 준비하되, 페니바실러스 일리노이센시스(Paenibacillus illinoisensis) 균주 대신 페니바실러스 티모넨시스(Paenibacillus timonensis) 균주를 사용하여 2.6×108 CFU/ml 농도의 혼합 균주 배양액을 준비하였다.A culture solution of the mixed strain was prepared by the method of Preparation Example 1, except that Paenibacillus lt; RTI ID = 0.0 > Paenibacillus < / RTI > timonensis ) was used to prepare a culture of mixed strains at a concentration of 2.6 x 10 8 CFU / ml.
비교예Comparative Example 3: 혼합 균주 배양액 준비 3: Preparation of mixed culture medium
상기 제조예 1의 방법으로 혼합 균주 배양액을 준비하되, 사카로미콥시스 스코에니(Saccharomycopsis schoenii) 균주 대신 사카로미콥시스 피부리제라(Saccharomycopsis fibuligera) 균주를 사용하여 2.6×108 CFU/ml 농도의 혼합 균주 배양액을 준비하였다.The culture of the mixed strain was prepared by the method of Preparation Example 1, except that Saccharomycopsis schoenii) instead of the Saccharomyces strain Rommie Cobb cis skin eluent La (Saccharomycopsis fibuligera ) was used to prepare a culture of the mixed strain at a concentration of 2.6 × 10 8 CFU / ml.
실시예Example 1: 혼합 균주 배양액 처리에 따른 암모니아 탈취 활성 분석 1: Analysis of ammonia deodorization activity by mixed culture medium treatment
본 발명의 균주가 암모니아에 대해 탈취 활성을 갖는지를 확인하기 위해, 균주 배양액 처리 후 시간변화에 따른 암모니아 농도를 조사하였다. 본 발명의 균주 배양액을 처리하지 않은 상태에서 시험을 진행한 것은 대조군으로 기재하였다. 각 균주 배양액을 멸균수로 5배 희석한 희석액 20 mL를 5 L의 반응기에 넣고 밀봉하였다. 각각 반응기에 시험가스인 초기 암모니아 농도를 50 μmol/mol으로 주입하고, 온도는 18~28℃에서, 습도는 40~60%를 유지하였으며, 시험가스의 농도를 초기(0분), 10분, 20분 및 30분에서 측정하였다. 시험가스의 농도는 검지관식 가스측정기(KS I 2218 : 2009)에 의해 측정하였다. 각 시간대별 탈취율은 다음과 같은 식에 의해 계산하였다; 탈취율(%)=[{(대조군 농도)-(균주 배양액 처리군 농도)}/(대조군 농도)]×100.In order to confirm whether the strain of the present invention has deodorizing activity against ammonia, the concentration of ammonia with time after the treatment of the culture broth was examined. Tests were carried out in the absence of the culture medium of the present invention as a control. 20 mL of the diluted solution of each culture broth 5 times diluted with sterilized water was put in a 5 L reactor and sealed. The initial ammonia concentration of 50 ol mol / mol was injected into the reactor, and the temperature was maintained at 18 ~ 28 캜 and the humidity was maintained at 40 ~ 60%. The initial concentration (0 min) 20 min and 30 min. The concentration of the test gas was measured by a detector tube gas analyzer (KS I 2218: 2009). The deodorization rate for each time period was calculated by the following equation; Deodorization rate (%) = [((control concentration) - (concentration of treated culture medium)} / (control concentration)] × 100.
그 결과 대조군에서 암모니아 농도는 거의 변화가 없었지만, 균주 배양액 처리군에서는 시간이 경과할수록 암모니아 농도가 현저하게 감소함을 확인할 수 있었다. 그 중 제조예 1의 혼합 균주 배양액 처리 시 30분 후 암모니아가 완전히 탈취되어, 가장 탈취 활성이 우수한 것을 확인할 수 있었다.As a result, there was almost no change in the ammonia concentration in the control group, but in the group treated with the strain, the ammonia concentration was remarkably decreased with time. Among them, ammonia was totally deodorized 30 minutes after the treatment of the mixed culture medium of Production Example 1, and it was confirmed that the deodorizing activity was the most excellent.
1) 단위: μmol/mol 1) Unit: μmol / mol
2) N.D.(not detected): 검출되지 않음(검출한계 0.2 μmol/mol) 2) ND (not detected): not detected (detection limit 0.2 μmol / mol)
실시예Example 2: 뱀장어 양식 실험 2: Eel culture experiment
뱀장어(Anguilla japonica)는 각 수조당 1,000마리(평균 100 g/마리)를 이용하였고, 사료는 시판중인 일반사료(제품명: 뱀장어 성만사료)를 투여하였고, 사료공급량은 뱀장어 체중의 1.5%를 공급하였다. 혼합 균주 배양액은 각 수조 물 1톤당 매일 30 mL씩 공급하면서 50일 동안 순환 여과방식으로 양식하였다. 수조의 온도는 25±1℃로 유지하면서 1일 환수량, pH, 아질산 및 암모니아 가스 농도를 측정하였고, 아질산 및 암모니아 가스 농도는 매일 오전 6시 환수 전 수조의 물을 채취하여 측정하였다. Eel (Anguilla japonica ) were used per 1,000 cows (average 100 g / horses). Feeds were fed on commercial commercial diets (product name: eel fish only) and feeds supplied 1.5% of eel weight. The culture of the mixed strains was cultured by circulation filtration for 50 days while supplying 30 mL per 1 ton of each water tank. PH, nitrite and ammonia gas concentrations were measured at 1 day while the temperature of the water tank was maintained at 25 ± 1 ℃. The concentrations of nitrite and ammonia gas were measured by taking water from the water tank before 6:00 am.
50일 동안 뱀장어를 양식하면서 균주 배양액을 투입한 결과, 제조예 1의 혼합 균주 배양액은 다른 균주 배양액의 투여에 비해 뱀장어 양식 시 발생하는 아질산 및 암모니아 가스를 현저하게 감소시켰고, 1일 환수량 역시 현저히 감소시킨 것을 확인할 수 있었다.As a result of culturing the eel for 50 days, the culture solution of the strain of Preparation Example 1 significantly reduced the nitrite and ammonia gas produced during eel culture compared to the culture of other strains, Respectively.
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