RU2491264C2 - Method for biological treatment of animal wastes - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: animal wastes are treated directly in animal farm facilities. A suspension of biomass of the Bacillus cereus 10.09.63 DEP strain deposited in the collection of the Russian State Centre for Quality and Standardisation of Veterinary Drugs and Feed is sprayed with number of healthy spore-forming bacteria Bacillus cereus of at least 2*10⁶ CFU/cm³, diluted with water in weight ratio of 1:60, respectively. The manure mass and manure effluent are collected and settled until separation into a liquid fraction and a solid fraction. After coagulation and clarification, the liquid fraction with pH not higher than 8.5 is used as biological fertiliser. The solid fraction is mixed with limed moss peat with pH not higher than 6.5 and coarse-grained sand with grain size of 1-3 mm with weight ratio of 1:(8-10):5. Soil with moisture content of not more than 65% is obtained.

EFFECT: invention simplifies recycling of animal wastes and enables to obtain biological fertiliser and soil.

1 dwg, 5 tbl, 6 ex

Description

The invention relates to agricultural biotechnology for processing animal waste and can be used for microbiological disposal of manure and manure directly in the premises of livestock (pig) farms and the production of biological fertilizers and soil.

A known method of preparing liquid manure of pig farms and farms for agricultural use by their reagent treatment with the release of sediment as an organic fertilizer, RU No. 2424985 C2, C02F 1/58, C05F 3/00, 07.27.2011; RU No. 2312819 C1, C02F 1/58, C02F 1/66, C05F 3/00, 12.20.2007.

A known bacterial strain of the genus Bacillus for fertilizer, RU No. 2408722 C1, C12N 1/20, C05F 11/08, 10.01.2011; RU No. 2105810 C1, C12N 1/20, B09C 1/10, C05F 3/00, C05F 11/08, 02.27.1998.

A known method of biological processing of bird droppings using bacteria strains of the genus Bacillus, RU No. 2376270 C2, C05F 11/08, 12/20/2009; RU No. 2322427 C1, C05F 11/08, C12N 1/20, 04/20/2008; RU No. 2266883 C2, C05F 11/08, C12N 1/20, 12/27/2005; RU No. 2197453 C1, C05F 11/08, 01/27/2003.

A known method of processing poultry manure and pig manure into organic fertilizer, comprising mixing poultry manure from poultry cage, poultry manure with bedding from poultry, pig manure and the introduction of the microbiological preparation "Baikal-EM1", RU No. 2409537 C1, C05F 3 / 01.20.01.2011.

A known method of treating manure in a barn, including layer-by-layer application of straw, accumulation of litter manure, separation of it into solid and liquid fractions, washing of hoofs with an aqueous solution of effective microorganisms of the Baikal EM-1 preparation, introduction of effective microorganisms into animal particles by the hoofs of animals, followed by mixing of manure and the production of rotted manure and liquid fraction, RU No. 2390516 C1, C05F 3/00, 05.27.2010.

A known method of utilization of fresh chicken manure to produce high-quality organic fertilizers, including the processing of fresh chicken manure with a solution of the biological product "Baikal-EM1" in non-chlorinated water in a ratio of 1: 100, which is carried out in the house on a conveyor belt during its preliminary treatment with the biological product "Baikal-EM1" , RU No. 2343137 C1, C05F 3/00, 01/10/2009.

There is a method of processing bedless manure of pig-breeding complexes, which consists in preparing an aqueous solution of the microbiological preparation "Tamir", spraying it in production rooms with subsequent separation of manure into liquid and solid fractions, RU No. 2406714 C2, C05F 3/00, 20.12.2010.

There is a method of preparing wastewater of pig breeding complexes and farms for agricultural use by sequentially introducing an alkaline coagulant and an acidifying reagent with the release of the resulting precipitate, RU No. 2357944 C1, C05F 3/00, 10.06.2009; RU No. 2321551 C1, C02F 1/58, C02F 1/66, 04/10/2008; RU No. 2312819 C1, C02F 1/58, C02F 1/66, 12.20.2007; RU No. 2242444 C1, C05F 3/00, C02F 1/58, 12.20.2004; RU No. 2132320 C1, C05F 9/00, 06/27/1999.

There is a method of disinfecting and detoxifying manure and bird droppings to ensure sanitary and hygienic working conditions in industrial premises and to reduce harmful emissions into the environment by detoxifying the liquid fraction in an electrolyzer with insoluble electrodes and treating the solid fraction with an ozone-air mixture and ultraviolet radiation, RU No. 2371425 C1, C05F 3/00, 10.27.2009.

A known method of purifying the air of livestock farms from harmful substances by passing air through a biologically active adsorbent containing a medium for the accumulation of microorganisms in the form of compost yeast prepared by cultivating a beer consortium of microorganisms isolated from well-humified humus layers of forest soil, consisting of mushrooms and bacteria genus Bacillus, including Bacillus subtilis, RU No. 2407781 C1, C12M 1/00, 12/27/2010.

A known method of preparing fertilizers from organic waste, including the separation of organic waste into liquid and solid fractions, RU No. 2235706 C2, C05F 3/00, 09/10/2004; RU No. 2101975 C1, C05F 3/00, 01.20.1998.

A known method of producing organic fertilizer based on peat, by processing fresh manure and subsequent mixing with peat, RU No. 2349565 C2, C05F 3/00, 03.20.2009; RU No. 2258686 C1, C05F 3/00, 08.20.2005.

It is known to obtain biological fertilizers in the processing of livestock waste and their widespread use in agriculture, "Scientific basis and practical recommendations for the use of biological fertilizers from livestock waste for biological farming" (Ed. Arkhipchenko IA), Recommendations of GNU VNIISKH of Microbiology, St. Petersburg, 2005 , 44 p.

Bacterial fertilizer is known, including a humic base in the form of peat and a bacterial supplement from the group of bacteria of the genus Bacillus, RU No. 2186049 C2, C05F 11/08, C12N 1/20, 07.27.2002; RU No. 2157798 C2, C05F 11/08, 10.20.2000.

Known soil used in green building for growing flowers and lawns, including horse peat, sand, mineral fertilizers, RU No. 2187928 C2, C05F 11/04, A01G 31/00, 08/27/2002.

A known method of preparing a peat substrate for growing seedlings of forest crops, including the introduction of a bioactivator, mixing and fermentation, RU No. 2365569 C1, C05F 11/02, C05F 9/04, C05F 11/08, 08/27/2009; RU No. 22251542 C1, C05F 11/02, 05/10/2005.

Known methods of disposal of animal and poultry waste, methods for producing biofertilizer, methods for preparing soil soils do not meet the requirements of solving the problem of the present invention.

A known method of biological processing of bird droppings, which consists in processing bird droppings with the bacterial culture of p.Bacillus and obtaining fertilizer, RU No. 2322427 C1, C05F 11/08, C12N 1/20, 04/20/2008.

This technical solution is made as the closest analogue of the present invention.

The closest analogue provides for the mixing of bird droppings with moisture-absorbing material, followed by aerobic fermentation of the mixture in the presence of microorganisms to obtain organic fertilizer. As microorganisms, a consortium of strains of Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 is used.

Bird droppings in the closest analogue are transferred to a trench and mixed with moisture-absorbing material - solid-phase droppings or peat. Fermentation is carried out for 6-8 days until the temperature of the fermentation mixture naturally drops to 25-30 ° C, after which the liquid culture of the consortium of bacteria No. VKPM B-5972 is introduced a second time and the fermentation is carried out for 2 days at a temperature of 20 ° C.

Fermentation allows you to get a microbial association with a high content of bacteria - plant growth stimulants, however, this method of waste processing is quite expensive and requires a long time to build systems for fermentation and commissioning.

Processing of bird droppings directly on the territory of the poultry complex in the nearest analogue is not provided.

The present invention is based on the solution of the problem, which allows to improve sanitary indicators and the environmental situation in the territories of livestock complexes, increase the economic indicators for livestock, facilitate the disposal of animal waste, and provide biofertilizer and soil.

The technical result of the present invention is to improve the operation of farms when using a bacterial strain of bacteria Bacillus cereus 10.09.63 DEPT deposited in the collection of VGNKI in the form of biomass when processing animal waste by spraying a suspension of biomass diluted with water directly in livestock buildings farms, when collecting, mixing and settling manure and manure waste before separation into liquid and solid fractions, when using the liquid fraction as biofertilizer, and firmly th fraction as soil soil.

According to the invention, this problem is solved due to the fact that the method of biological processing of animal waste consists in treating animal waste with a bacterial culture of the genus Bacillus and obtaining fertilizer.

As a bacterial culture, the Bacillus cereus strain 10.09.63 DEPT, deposited in the collection of FGU “VGNKI”, in the form of biomass suspended in a nutrient medium, with the number of viable spore-forming bacteria Bacillus cereus at least 2 * 10 ⁶ CFU / cm ^{3, is used} .

The treatment of animal waste is carried out directly in the premises of livestock farms by spraying a suspension of biomass of the strain Bacillus cereus 10.09.63 DEPT diluted with water in a mass ratio of 1:60, respectively.

Treatment of animal waste is carried out by collecting and mixing manure and manure and settling them before separation into liquid and solid fractions.

The liquid fraction with a pH of not more than 8.5 after coagulation and clarification is used as biofertilizer.

The obtained solid fraction is used to obtain soil with a mass fraction of moisture of not more than 65%.

Soil soil is obtained by mixing the solid fraction with calcareous peat at a pH of not more than 6.5 and coarse sand 1-3 mm, while the solid fraction, calcareous peat and coarse sand are taken in a mass ratio of 1: (8-10): 5.

The applicant has not identified sources containing information about technical solutions identical to the present invention, which allows us to conclude that it meets the criterion of "novelty."

Due to the implementation of the distinguishing features of the invention (together with the features indicated in the restrictive part of the formula), important new properties of the object are achieved.

The use of bacteria culture in the form of a Bacillus cereus strain 10.09.63 DEPT deposited in the collection of FGU “VGNKI” when processing animal waste, reduces the concentration of ammonia in the air of the working area, improves the working conditions of staff, provides more comfortable conditions for keeping animals, which improves sanitary conditions indicators and environmental conditions in the territory of livestock complexes.

The treatment of animal waste directly in the premises of livestock farms by spraying a suspension of biomass of the strain Bacillus cereus 10.09.63 DEPT diluted with water, collecting and mixing manure and manure drains facilitates the disposal of animal waste, increases economic indicators for livestock, leads to increased immunity in animals, increase weight gain, reducing the mortality of young animals and reducing the timing of fattening.

Conducting the sedimentation of manure and manure, treated with a suspension of biomass of the strain Bacillus cereus 10.09.63 DEPT diluted with water, before separation into liquid and solid fractions and using the liquid fraction as biofertilizer, and the solid fraction for the preparation of soil.

The preparation of soil by mixing the solid fraction with calcareous peat and coarse sand at optimal proportions ensures the production of soil.

The applicant is not aware of any publications that would contain information on the influence of the distinguishing features of the invention on the achieved technical result. In this regard, according to the applicant, it can be concluded that the claimed technical solution meets the criterion of "inventive step".

The method is as follows.

Recycling of animal waste is carried out by a bacterial culture of the strain Bacillus cereus 10.09.63 DEPT deposited in the collection of FGU "VGNKI".

The strain Bacillus cereus 10.09.63 DEPT consists of biomass suspended in a nutrient medium.

As a culture medium using any of the known culture media.

The biomass of the strain Bacillus cereus 10.09.63 DEPT has a number of viable spore-forming bacteria Bacillus cereus not less than 2 * 10 ⁶ CFU / cm ³ .

The strain Bacillus cereus 10.09.63 DEPT was deposited at the FSI "VGNKI" 04/18/2011.

The treatment of animal waste is carried out directly in the premises of livestock farms by spraying a suspension of biomass of the strain Bacillus cereus 10.09.63 DEPT diluted with water in a mass ratio of 1:60, respectively.

Treatment of animal waste is carried out by collecting and mixing manure and manure and settling them before separation into liquid and solid fractions.

The liquid fraction with a pH of not more than 8.5 after coagulation and clarification is used as biofertilizer.

Biofertilizer may be diluted when used.

The obtained solid fraction is used to obtain soil with a mass fraction of moisture of not more than 65%.

Soil soil is obtained by mixing the solid fraction with calcareous peat at a pH of not more than 6.5 and coarse sand 1-3 mm.

The solid fraction, calcareous peat and coarse sand are taken in a mass ratio of 1: (8-10): 5.

The implementation of the method is confirmed by examples 1, 2, 3, 4, 5, 6.

Example 1

The method of obtaining the strain: Bacillus cereus bacterial strain 10.09.63 DEPT obtained by selection of natural microorganisms and is not a genetically modified microorganism.

Brief description of the bacterial strain Bacillus cereus 10.09.63 DEPT:

Aerobic spore-forming sticks, straight or slightly curved, 0.5-0.7 × 3.0-7.0 microns. Gram-positive, catalase-positive. The spore is oval, central, sporangium is not convex. Colonies on meat and peptone agar are milky white, mucous. With growth, a surface film forms on the meat-peptone broth. The strain ferments glucose, ribose, arabinose, sucrose. Does not use sorbitol, rhamnose, lactose. Hydrolyzes starch, gelatin.

Source of origin (isolation) of the bacterial strain Bacillus cereus 09/10/63 DEPT: soil of the Leningrad region

The identification of the strain Bacillus cereus 10.09.63 DEPT was carried out using the molecular genetic method based on sequencing and the search for the closest representatives from international databases.

Data on the DNA sequence characterizing the strain of Bacillus cereus 10.09.63 DEPT, are presented in figure 1.

The production of the bacterial strain Bacillus cereus 10.09.63 DEPT includes the following stages:

- storage of the production strain;

- growing the initial culture of bacteria in the laboratory;

- preparation of an inoculating culture of bacteria;

- preparation of a sterile nutrient medium in production volumes;

- the introduction of an inoculating culture of bacteria in a production medium;

- deep cultivation of bacteria;

- bottling of microbial mass in transport containers, labeling of finished products;

- transportation of finished products to the warehouse and its storage.

The cultivation of the strain Bacillus cereus 10.09.63 DEPT is carried out in the laboratory.

For the cultivation of the strain Bacillus cereus 10.09.63 DEPT, various sources of carbon are used - filter paper, hay, straw, meal, cake.

The strain Bacillus cereus 10.09.63 DEPT has a high cellulolytic activity (decomposes filter paper for 3 days by 90%).

As nutrient media, various types of media can be used, including various sources of carbon and nitrogen, organic and inorganic salts, which are widely used to obtain a culture of ordinary bacteria.

The strain Bacillus cereus 10.09.63 DEPT can multiply in the gastrointestinal tract of animals, while providing a positive aftereffect in its application.

The strain Bacillus cereus 10.09.63 DEPT has no toxicity and pathogenic properties for animals.

Physico-chemical and microbiological control is carried out at all stages of the production of the strain Bacillus cereusl0.09.63 DEPT.

The determination of appearance and color is carried out visually in daylight diffused light or under illumination with incandescent or fluorescent lamps.

The resulting preparation, based on the Bacillus cereus strain 10.09.63 DEPT, is a light brown to dark brown liquid with a sediment of a nutrient medium and a specific odor.

White surface film may be present.

The drug is packaged from 1 dm ³ to 10 dm ³ in an airtight container made of polymeric materials according to GOST R 51760.

The drug can be made in the form of a powder.

Volatile components of the drug do not have a pronounced irritant effect on the mucous membrane of the upper respiratory tract.

The drug does not have pathogenic properties: it is not virulent, non-toxic, not toxigenic, does not cause desimination in the internal organs of warm-blooded animals - hazard class 4 (not dangerous)

The drug based on the bacterial strain Bacillus cereus 10.09.63 DEPT is safe for humans, animals and the environment, does not form toxic compounds in wastewater and air.

When working with the drug, you must follow the general rules of personal hygiene and safety.

Each unit of preparation of the drug is supplied with instructions for use and a label indicating: the name of the manufacturer, its address, name, quantity, batch number, control number, production date (number, month, year), shelf life, storage conditions, TU designation , inscriptions "For animals".

Storage of the drug packaging is carried out in a dry, dark place at a temperature of 0 ° to 20 ° C.

Shelf life - 5 months from the date of manufacture.

The drug based on the bacterial strain Bacillus cereus 10.09.63 DEPT after the expiration date is not subject to use.

Example 2

Determination of bacterial content in the culture of the strain Bacillus cereus 10.09.63 DEPT.

The determination of the number of viable spore-forming bacteria Bacillus cereus 10.09.63 DEPT is carried out using the following equipment, materials and reagents:

- test tubes in accordance with GOST 25336, GOST 1770-74;

- pipettes according to GOST 29227;

- Petri dishes according to GOST 25336;

- thermostat of any type, providing a temperature (32 ± 1 ° C);

- 0.9% sodium chloride solution;

- sodium chloride according to GOST 4233;

- distilled water according to GOST 6709;

- meat and peptone agar according to GOST 29112.

Examine three samples of each batch.

Of the samples taken for analysis, after thorough shaking for 10 minutes, 10 cm ^{3 of} liquid are taken with a sterile pipette and transferred to a flask with 90 cm ^{3 of a} 0.9% sterile sodium chloride solution.

An initial dilution of 10 ^{-1 is} obtained.

After five minutes of agitation, dilutions from 10 ^-2 to 10 ^{-5 are} prepared by serial ten-fold dilution, with a separate sterile pipette being used in each case.

20 cm ^{3 of} molten meat-peptone agar are poured into sterile Petri dishes and left to solidify the nutrient medium.

From a dilution of 10 ^-4 and 10 ^-5 make seeding of 0.1 cm ³ in three Petri dishes for each dilution, rub the material with a sterile glass spatula on the surface of the medium.

Then the Petri dishes are incubated at a temperature of 32 ° C for 24 hours.

At the end of the incubation, dilutions in which there is a characteristic growth of the colonies are noted, and the number of cells in 1 cm ^{3 of the} preparation is calculated by the formula:

X = ((a ₁ + a ₂ + a ₃ ) * 10 ⁿ ) / 3, where

X is the number of colony forming units of bacteria,

a ₁ , a ₂ , and ₃ - the number of colonies in the considered breeding,

10 ⁿ - breeding ratio, which takes into account growth.

Determination of the microbiological purity of the strain Bacillus cereus 10.09.63 DEPT.

The presence of bacteria in the culture of the strain Bacillus cereus 10.09.63 DEPT is established in accordance with the "Rules of bacteriological research of feed", approved. GUV Ministry of Agriculture of the USSR from 10.06.75 g.

Example 3

The safety of the strain Bacillus cereus 10.09.63 DEPT.

Determination of the biomass safety of the strain Bacillus cereus 10.09.63 DEPT is carried out using the following materials and reagents:

- medical syringe according to GOST 22967;

- a needle with deposited olive with a diameter of 1-2 mm according to GOST 25337-93;

- 0.9% sodium chloride solution according to GF X;

- white mice weighing 18-20 g.

Prepare a working solution.

In 1.0 cm ^{3 the} biomass of the strain Bacillus cereus 10.09.63 DEPT diluted in 5.0 cm ³ sterile 0.9% sodium chloride solution.

Within five days, five outbred mice weighing 18-20 g, using a needle on the end of which an olive with a diameter of 1.0-2.0 mm is fused, enter 0.5 cm of the prepared working solution intragastrically. Parallel to five control mice, 0.5 cm of a sterile 0.9% sodium chloride solution used to prepare the working solution was intragastrically administered.

Mice are monitored for 7 days.

A drug based on the bacterial strain Bacillus cereus 10.09.63 DEPT is considered harmless if during the observation period all mice from the experimental and control groups remain alive and clinically healthy.

If one or more mice die, the test is repeated at twice their number. With the death of at least one mouse in a repeated experiment, the drug is rejected.

Example 4

Preparation of a biologically active aqueous solution based on the biomass of the strain Bacillus cereus 10.09.63 DEPT.

In 1 dm ^{3 the} biomass of the strain of Bacillus cereus 10.09.63 DEPT is dissolved in 60 dm ^{3 of} water.

The prepared bio-solution is used to spill the lattice floors of sections in the rooms where the animals are located.

The approximate consumption per animal is 170-190 cm ^{3 of} bio-solution for 1 treatment.

The bio-solution contains the biomass of the bacterial strain Bacillus cereus 10.09.63 DEPT and water with a mass ratio of biomass and water as 1:60, respectively.

The rate of input of bio-solution is: 3 dm ^{3 of} bio-solution per 1000 heads.

Processing is carried out according to the following scheme:

1st month - 4 treatments;

2nd month - 3 treatments;

subsequent months - 2 treatments.

Bio-solution is used to treat animal welfare directly in the premises of livestock farms with the aim of processing animal waste and to remove odors of organic origin on the territory of industrial and agricultural complexes.

The bio-solution was used for the disposal of wastewater at the pig farm "New World" of the Gatchinsky district, Leningrad region.

Physico-chemical properties of manure effluent before and after application of the drug based on the strain of Bacillus cereus 10.09.63 DEPT are presented in table 1 in figure 2.

From table 1 it can be seen that the analysis of samples of animal pig waste before introduction of the drug and 12 days after it is significantly different, which is especially noticeable when assessing the fractional composition of animal waste selected before and after treatment with the drug.

The content of total nitrogen (Ntotal) as a result of decomposition processes decreased by 765 mg / L, and the amount of ammonium nitrogen (N-NH ₄ ) increased by 730 mg / L. Based on this, it can be concluded that the ammonia released during the decomposition of organic nitrogen-containing compounds was fixed in the dung mass of animal waste, which contributed to a decrease in its concentration in the air.

The acidity of animal waste samples after application of the drug due to an increase in the amount of ammonium nitrogen has changed from a slightly acid value to a weakly basic value.

The content of gross phosphorus in the initial sample of animal waste was very high - 5077 mg / l. Virtually all phosphorus was present as insoluble compounds. After treatment of animal waste with the preparation, the amount of total phosphorus in the shaken sample, as well as in the solid fraction, decreased by 5.5 times. The same pattern was observed with respect to potassium - the introduction of the drug reduced the total potassium content in the shaken sample by 5.5 times.

Side effects and complications when using a biologically active solution with a preparation based on the Bacillus cereus strain 10.09.63 DEPT were not detected.

The drug based on the Bacillus cereus strain 10.09.63 DEPT reduces the concentration of ammonia in the air of the working area, improving the working conditions of staff.

When using a preparation based on the Bacillus cereus strain 10.09.63 DEPT, the conditions for keeping animals become more comfortable, their immunity increases, weight gain increases, the mortality rate of young animals decreases, and the duration of feeding.

Air sampling is carried out at a height of 1.0-1.5 meters from the floor. Measurements are carried out using special laboratory equipment: an aspirator for sampling air. Model A-01 Head No. 146.

The ammonia concentration in the pig housing is presented in table 2 in figure 3.

As a result of measurements in the treated room, a dynamic decrease in the level of ammonia was noted: from 7.2 to 4.6 mg / cubic meter or by 36.0%.

Economic indicators for livestock when using the drug are presented in table 3 in figure 4.

Table 3 shows that the use of the drug for the disposal of animal waste directly in the livestock building shows a significant improvement in the sanitary condition and microclimate.

The safety of the livestock increased by 3.0%.

The average daily gain in the experimental group increased by 8.6% compared with the control group.

Reduction of fattening by 12 days, which amounted to 19800 fodder.

Example 5

Biofertilizer research.

Biofertilizer is characterized by the following indicators: pH not more than 8.5, total nitrogen content not less than 1.0 g / l, ammonium nitrogen not more than 1.5 g / l, total phosphorus (on P ₂ O ₅ ) not less than 200 mg / l total potassium (at K ₂ O) not less than 200 mg / l.

Experimental biofertilizer samples are examined under laboratory conditions on a light installation (illumination of 2000 lux) in plastic vessels with a volume of 2 l (parsley) and 3 l (salad).

The control was neutralized peat: humidity 58%; ash content of 9%; pH _KCl 6.28; bulk density 285 g / l.

Biofertilizer is applied in two doses: the first at the rate of 3 l of biofertilizer per m ² ; The second at the rate of 6 liters per m ² , which corresponds to 100 and 200 mg of nitrogen per liter of soil.

The effect of biofertilizer on the crop of green crops in action and aftereffect is presented in table 5 in Fig.6.

Table 5 shows both in action and in the aftermath, the yield of green crops (Italian giant parsley, Malvina salad) significantly increases compared to the control, which confirms the advisability of using the liquid fraction obtained after sludge as biofertilizer.

Example 6

Soil preparation.

The solid fraction is used for the preparation of soil.

The solid fraction is mixed with lime peat at a pH of not more than 6.5 and an ash content of not more than 30% and coarse sand 1-3 mm.

The solid fraction, calcareous peat and coarse sand are taken in a mass ratio of 1: 8: 5.

Soil soil is characterized by the following indicators: mass fraction of moisture not more than 65%, ash content not more than 40%, pH _KCl not more than 7.0, and total dry nitrogen content not less than - 0.6%, mobile phosphorus (by P ₂ O ₅ ) not less than 200 mg / l and potassium (K ₂ O) not less than 150 mg / l.

Tests of soil are carried out in plastic vessels with a volume of 2 l on a light installation (illumination of 2000 lx).

Soil soil (prototype) is compared with commercial soil (Seliger-Agro LLC), with lime peat without fertilizers and with peat enriched with mineral fertilizers.

The analysis of wet weight in both the first and second mowing showed a significant advantage of the prototype compared to commercial and peat without fertilizers, especially sharp differences appeared during the second mowing. The length of grass in the second mowing was also maximum when compared with all tested options. Such a bright advantage can be explained by the gradual decomposition of the organic fraction of manure and the higher saturation of the prototype with nutrients.

The experimental data on the growth of lawn grass on various soil substrates are presented in table 4 in figure 5.

Additionally, animal waste is treated with a biological active solution, sedimented, and the solid fraction is mixed with calcareous peat and coarse sand in a mass ratio of 1: 9: 5 and 1: 10: 5, respectively.

The experimental data on the growth of lawn grass of these soil soils are similar to those in table 4.

The obtained results confirm the feasibility of microbiological disposal of manure in livestock farms and the use of decomposition products for the production of effective biological fertilizers and soil.

The implementation of the method of processing animal waste with biomass of the strain Bacillus cereus 10.09.63 DEPT provides microbiological disposal of manure and manure.

The strain Bacillus cereus 10.09.63 DEPT is the basis of a wide range of biological products, such as probiotics, preparations for the decomposition of manure and preparations for improving the microclimate in places where animals are kept in pig farms and livestock farms.

The proposed method for the biological treatment of animal waste in the production of biofertilizer and soil is made according to well-known biotechnologies that are widely used in agriculture, and the experimental work on the farms of pig farms in the Leningrad Region determines, according to the applicant, its compliance with the criterion of "industrial applicability".

Using the proposed method allows you to:

- improve sanitary indicators and environmental conditions in the territories of livestock complexes;

- reduce the concentration of ammonia in the air of the working area;

- improve working conditions for staff;

- improve animal welfare;

- facilitate the process of removal and processing of manure and manure;

- provide biofertilizer;

- ensure the receipt of soil.

Claims (1)

A method of biological processing of animal waste, which consists in treating animal waste with a bacterial culture of the Bacillus genus and producing fertilizer, characterized in that the bacterial strain is Bacillus cereus strain 10.09.63 DEPT, deposited in the collection of FGU VGNKI, in the form of biomass suspended in medium, with the amount of viable Bacillus cereus spore forming bacteria of at least 2 × 10 ⁶ CFU / cm ^3, the processing of animal waste is carried out directly on the premises of farm animals by spraying a suspension of biomass tamma Bacillus cereus 10.09.63 DEPT diluted with water at a mass ratio of 1:60, respectively, collecting and mixing the manure and manure and settling them before separation into liquid and solid fractions, of which the liquid fraction with a pH of not more than 8.5 after coagulation and clarification are used as biofertilizer, and the solid fraction is used to obtain soil with a mass fraction of moisture of not more than 65% by mixing the solid fraction with calcareous peat at a pH of not more than 6.5 and coarse sand 1-3 mm, while solid fraction, lime woven peat and coarse sand are taken at a mass ratio of 1: (8-10): 5.

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