CN112522159A - Microbial compound microbial inoculum for treating peculiar smell of garbage throwing point - Google Patents
Microbial compound microbial inoculum for treating peculiar smell of garbage throwing point Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/72—Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention is applicable to the technical field of garbage peculiar smell treatment, and provides a microbial compound microbial inoculum for garbage throwing point peculiar smell treatment, which comprises a bacillus complex, an activated lactobacillus group, pseudomonas putida and a microbial inoculum carrier. Through compounding the bacillus, the complexing agent with the diversified degradation function is obtained, the lactobacillus group is activated, the activity of the group and the efficiency of decomposing organic matters are improved, and the synergistic effect of the pseudomonas putida is matched, so that the removal rate of the malodorous substances is greatly improved, and a relatively ideal odor removing effect can be achieved.
Description
Technical Field
The invention relates to the technical field of garbage odor treatment, in particular to a microbial compound microbial inoculum for garbage throwing point odor treatment.
Background
The peculiar smell of the garbage is generated by decomposing organic components in the garbage by bacteria, and the organic matters in the household garbage generate stink under the action of aerobic fermentation, anaerobic fermentation and the like in the natural digestion process. The peculiar smell of the garbage comprises ammonia gas, hydrogen sulfide, organic amine, organic sulfur, volatile fatty acid and the like. The peculiar smell of the garbage not only enables a large number of bacteria for spreading diseases to multiply and propagate, but also easily stimulates olfactory organs of people, directly generates strong stimulation to a respiratory system, a nervous system, a circulatory system and an endocrine system through the olfactory system, and becomes environmental pollution. Landfill sites, refuse transfer stations and refuse disposal stations are the main locations where malodorous gases are generated.
With the rapid development of social economy and the continuous acceleration of urbanization process, the problem of environmental pollution caused by garbage odor is increasingly prominent, the quality of life of people is directly affected, even the health of people is harmed, and the odor pollution treatment is very urgent. The main methods for treatment in the past are physical, combustion, chemical oxidation, absorption, adsorption and combination. Most of the methods have the defects of high investment, huge required equipment, difficult operation, complex process and the like, and are difficult to be widely applied in the actual deodorization process. At present, researchers begin to research on removal of malodorous substances by a microbial filtration method, but the removal rate of malodorous substances in the currently disclosed method is low, the investment and operation cost is high, and the method is not suitable for large-area popularization.
At present, the emerging biological deodorization method mainly utilizes microorganisms to deodorize, substances with odor are converted through physiological metabolism of the microorganisms, and the biological deodorization technology is gradually developed in the field of environmental management by virtue of incomparable superiority. Can be used for eliminating swill treatment, garbage treatment, animal manure treatment, composite fertilizer composting, livestock and poultry breeding, septic tanks, toilets, oil stain pools, sewers, garbage cans, garbage trucks, restaurants, hotels, habitable rooms, hospitals, municipal administration, agriculture and the like to generate ammonia gas, hydrogen sulfide and other toxic and harmful gases for control and deodorization. The microbial and mildew degradation is utilized to generate toxic and harmful gases such as ammonia gas, hydrogen sulfide and the like, the generation of the harmful gases is controlled from the source, and the harmful gases are eliminated. However, the existing biological deodorization method adopted in the odor treatment of the garbage throwing point has low removal rate of malodorous substances, cannot produce ideal odor removal effect, and is not suitable for large-area popularization.
Disclosure of Invention
The invention provides a microbial compound microbial inoculum for treating peculiar smell of a garbage throwing point, which aims to compound bacillus and obtain a compound body with a diversified degradation function; meanwhile, the lactobacillus flora is activated, so that the activity of the flora and the efficiency of decomposing organic matters are improved, and the synergistic effect of the pseudomonas putida is matched, so that the removal rate of malodorous substances is improved, and a relatively ideal odor removing effect is achieved.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a microbial compound microbial inoculum for treating peculiar smell of a garbage throwing point comprises a bacillus complex, an activated lactobacillus group, pseudomonas putida and a microbial inoculum carrier.
Further, the bacillus complexing agent is prepared by compounding bacillus licheniformis, bacillus cereus and bacillus subtilis.
Further, the activated lactic acid bacteria group comprises the following components in a mass ratio of (1-5): (3-5): (2-6) Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium.
Further, the microbial inoculum carrier comprises peptone, glucose, tea polyphenol, amino acid and vitamins.
Further, the effective viable count of the pseudomonas putida is more than or equal to 109one/mL.
Further, the mass ratio of the bacillus licheniformis, the bacillus cereus and the bacillus subtilis in the bacillus complexing agent is (3-5): (1-3): (3-5).
Further, the preparation method of the microbial inoculum carrier comprises the following steps: adding peptone, glucose, tea polyphenols, amino acids and vitamins into warm water of 50-60 deg.C, stirring thoroughly to dissolve completely, and cooling to 37-42 deg.C to obtain microbial inoculum carrier.
Further, the contents of the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are (5-20) g/L, (20-40) g/L, (1-5) g/L, (30-50) g/L and (10-20) g/L, respectively.
The invention also provides the microbial compound microbial inoculum for treating the peculiar smell of the garbage throwing point, which is realized by the following steps:
1) compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to a mass ratio;
2) performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium to obtain an activated lactobacillus group;
3) respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Further, the concentration of each bacterium is not less than 107CFU/mL。
The invention has the following beneficial effects:
according to the invention, the bacillus is compounded to obtain the complexing agent with a diversified degradation function, the lactobacillus group is activated to improve the activity of the group and the efficiency of decomposing organic matters, and the synergistic effect of the pseudomonas putida is matched, so that the removal rate of malodorous substances is greatly improved, and a more ideal odor removing effect can be achieved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The embodiment of the invention provides a microbial compound microbial inoculum for treating peculiar smell of a garbage throwing point, which comprises a bacillus complex, an activated lactobacillus group, pseudomonas putida and a microbial inoculum carrier.
In the embodiment of the invention, the bacillus complexing agent is prepared by compounding bacillus licheniformis, bacillus cereus and bacillus subtilis.
In the embodiment of the invention, the activated lactic acid bacteria group comprises the following components in a mass ratio of (1-5): (3-5): (2-6) Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium.
In the embodiment of the invention, the microbial inoculum carrier comprises peptone, glucose, tea polyphenol, amino acid and vitamin.
In the embodiment of the invention, the effective viable count of the pseudomonas putida is more than or equal to 109/mL.
In the embodiment of the invention, the mass ratio of bacillus licheniformis, bacillus cereus and bacillus subtilis in the bacillus complexing agent is (3-5): (1-3): (3-5).
Further, the preparation method of the microbial inoculum carrier comprises the following steps: adding peptone, glucose, tea polyphenols, amino acids and vitamins into warm water of 50-60 deg.C, stirring thoroughly to dissolve completely, and cooling to 37-42 deg.C to obtain microbial inoculum carrier.
In the examples of the present invention, the contents of peptone, glucose, tea polyphenol, amino acid and vitamin are (5-20) g/L, (20-40) g/L, (1-5) g/L, (30-50) g/L and (10-20) g/L, respectively.
The invention also provides the microbial compound microbial inoculum for treating the peculiar smell of the garbage throwing point, which is realized by the following steps:
1) compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to a mass ratio;
2) performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium to obtain an activated lactobacillus group;
3) respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
In the embodiment of the invention, the concentration of each bacterium is not less than 107 CFU/mL.
The technical solution and the technical effect of the present invention will be further described by specific examples.
Example 1
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 2
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 4:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 3
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 5:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 4
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:2: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 5
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 1:1: 1; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 6
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 4; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 7
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 5; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 8
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 3:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 9
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 5:3:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 10
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:4:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 11
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:5:2 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 12
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:4 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Example 13
Adding 10g of peptone, 30g of glucose, 3g of tea polyphenol, 40g of amino acid and 15g of vitamin into 1L of warm water at the temperature of 50 ℃, fully stirring until the peptone, the glucose, the tea polyphenol, the amino acid and the vitamin are completely dissolved, and then cooling to 40 ℃ to obtain a microbial inoculum carrier; then compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to the mass ratio of 3:1: 3; then performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium according to the mass ratio of 1:3:6 to obtain an activated lactobacillus group; and finally, respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
Examples of the experiments
The microbial compound inocula prepared in examples 1-13 are respectively taken and numbered, each group of inocula are respectively put into the same garbage sample in equal amount, the odor grade is measured by a sensory method after ten days, and the measurement results are shown in table 1.
Wherein, the 0 level: no odor, indicated as "-"; level 1: the odor is barely felt and is indicated by "+"; and 2, stage: faint odor, indicated by "+"; and 3, level: the odor is obvious and is indicated by "+ + +"; 4, level: strong odor, indicated by "+ + + + +"; and 5, stage: the odor is intolerable, indicated by "+ + + + +".
TABLE 1
| Numbering | Detecting items | Grade of malodor |
| 1 | Example 1 | ++ |
| 2 | Example 2 | + |
| 3 | Example 3 | ++ |
| 4 | Example 4 | ++ |
| 5 | Example 5 | +++ |
| 6 | Example 6 | + |
| 7 | Example 7 | ++ |
| 8 | Example 8 | + |
| 9 | Example 9 | +++ |
| 10 | Example 10 | ++ |
| 11 | Example 11 | ++ |
| 12 | Examples12 | + |
| 13 | Example 13 | +++ |
Further, the removal rates of ammonia gas and hydrogen sulfide gas in the treated refuse were measured, respectively, and the measurement results are shown in table 2.
TABLE 2
| Numbering | Detecting items | Removal rate of Ammonia (%) | Hydrogen sulfide removal Rate (%) |
| 1 | Example 1 | 68.4 | 49.3 |
| 2 | Example 2 | 72.5 | 53.2 |
| 3 | Example 3 | 66.5 | 45.7 |
| 4 | Example 4 | 63.2 | 43.4 |
| 5 | Example 5 | 58.7 | 39.5 |
| 6 | Example 6 | 73.8 | 52.4 |
| 7 | Example 7 | 64.9 | 46.3 |
| 8 | Example 8 | 75.4 | 56.5 |
| 9 | Example 9 | 53.6 | 36.8 |
| 10 | Example 10 | 64.3 | 43.9 |
| 11 | Example 11 | 67.7 | 48.3 |
| 12 | Example 12 | 74.8 | 53.6 |
| 13 | Example 13 | 56.5 | 38.2 |
As can be seen from the data in tables 1 and 2, the prepared microbial compound microbial inoculum has a good deodorization effect in the garbage odor treatment process, wherein the deodorization effects of the embodiments 2, 6, 8 and 12 are good, especially in the embodiment 8, the ammonia gas removal rate reaches over 75%, the hydrogen sulfide removal rate reaches over 55, and the best deodorization performance is shown.
In general, the bacillus is compounded to obtain the complexing agent with the diversified degradation function, the lactobacillus group is activated to improve the activity of the group and the efficiency of decomposing organic matters, and the synergistic effect of the pseudomonas putida is matched, so that the removal rate of the malodorous substances is greatly improved, and the ideal odor removing effect can be achieved.
It should be understood that the above-mentioned embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A microbial compound microbial inoculum for treating peculiar smell of a garbage throwing point is characterized by comprising a bacillus complex, an activated lactobacillus group, pseudomonas putida and a microbial inoculum carrier.
2. The microbial compound microbial inoculum for odor control of a garbage throwing point according to claim 1, which is characterized in that the bacillus complexing agent is prepared by compounding bacillus licheniformis, bacillus cereus and bacillus subtilis.
3. The microbial compound microbial inoculum for odor control of a garbage throwing point according to claim 2, which is characterized in that the activated lactobacillus group comprises the following components in percentage by mass (1-5): (3-5): (2-6) Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium.
4. The microbial compound microbial inoculum for odor control of a garbage throwing point according to claim 1, wherein the microbial inoculum carrier comprises peptone, glucose, tea polyphenol, amino acids and vitamins.
5. The microbial compound inoculant for odor control of a garbage throwing point as claimed in claim 1, wherein the effective viable count of the pseudomonas putida is more than or equal to 109one/mL.
6. The microbial compound microbial inoculum for odor control of a garbage throwing point according to claim 2, wherein the mass ratio of bacillus licheniformis, bacillus cereus and bacillus subtilis in the bacillus complexing agent is (3-5): (1-3): (3-5).
7. The microbial compound microbial inoculum for treating the odor of the garbage throwing point according to claim 3, which is characterized in that the preparation method of the microbial inoculum carrier comprises the following steps: adding peptone, glucose, tea polyphenols, amino acids and vitamins into warm water of 50-60 deg.C, stirring thoroughly to dissolve completely, and cooling to 37-42 deg.C to obtain microbial inoculum carrier.
8. The microbial compound inoculant for treatment of odor in a garbage throwing site as claimed in claim 7, wherein the contents of peptone, glucose, tea polyphenol, amino acids and vitamins are (5-20) g/L, (20-40) g/L, (1-5) g/L, (30-50) g/L and (10-20) g/L, respectively.
9. The microbial compound inoculant for odor control of a garbage throwing point as claimed in claim 7 or 8, which is realized by the following steps:
1) compounding bacillus licheniformis, bacillus cereus and bacillus subtilis into a bacillus complexing agent according to a mass ratio;
2) performing slant activation on lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium to obtain an activated lactobacillus group;
3) respectively inoculating the bacillus complexing agent, the activated lactobacillus group and the pseudomonas putida to a microbial agent carrier to obtain the microbial compound microbial agent.
10. The microbial compound inoculant for odor control of a garbage throwing point according to claim 9, wherein the concentration of each bacteria is not less than 107CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011527551.5A CN112522159A (en) | 2020-12-22 | 2020-12-22 | Microbial compound microbial inoculum for treating peculiar smell of garbage throwing point |
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| CN114891662A (en) * | 2022-03-05 | 2022-08-12 | 福建亮洁环境服务有限公司 | Pseudomonas putida and application thereof |
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