CN110129236A - A kind of preparation method of microbial deodorant - Google Patents
A kind of preparation method of microbial deodorant Download PDFInfo
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- CN110129236A CN110129236A CN201910461722.XA CN201910461722A CN110129236A CN 110129236 A CN110129236 A CN 110129236A CN 201910461722 A CN201910461722 A CN 201910461722A CN 110129236 A CN110129236 A CN 110129236A
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Abstract
The present invention provides a kind of preparation method of microbial deodorant, the following steps are included: the saccharomycetes to make fermentation liquid being prepared is added in pail pack, the lactic acid bacteria first order seed being prepared is inoculated into saccharomycetes to make fermentation liquid and is further fermented, herein, using the directly further fermentative lactobacillus first order seed of saccharomycetes to make fermentation liquid；Wherein, fermentation are as follows: pail pack pressure is normal pressure, and 25~35 DEG C of temperature, fermentation time 60~96 hours, the inoculum concentration of lactic acid bacteria first order seed was 2~3% mass fractions, and microbial deodorant is finally prepared.Advantage are as follows: the present invention provides a kind of preparation method of microbial deodorant, it is the microbial deodorant that a kind of production method is simple, pollution-free, efficient, safe, by saccharomycete and lactic acid bacteria mixed fermentation, significantly improve the deodorizing effect of microbial deodorant, the problem of solving traditional chemical deodorant deodorization to be not thorough, polluting environment.
Description
Technical field
The invention belongs to microbial deodorant technical fields, and in particular to a kind of preparation method of microbial deodorant.
Background technique
Foul gas not only causes to seriously affect to ecological environment, but also has greatly harm to human health, can make
Nervous centralis generates obstacle, lesion, causes chronic disease, acute disease.Foreign countries have just started effluvium early in late 1950s
The research that body pollution is administered, and have accumulated theoretical knowledge abundant and practical experience.Just carry out stench the 1980s in China
Research in terms of the investigation of gaseous contamination, test and standard, and to the research of deodorization technique just opened from the 1990s
Begin to carry out.
The purpose of various foul gas processing methods is the effect by physics, chemistry, biology, makes the object of foul gas
Matter structure changes, and eliminates stench.Conventional foul gas processing method have combustion method, oxidizing process, absorption process, absorption method,
Neutralisation and bioanalysis etc..
Microbiological treatment foul smell be using peculiar microorganism metabolic activity by sulfur-containing compound, nitrogenous compound, halogen and
Its derivative etc. has the pernicious gas degradation of foul smell or is converted into harmless odorless substance, and reaching improves air quality, protects
Protect the purpose of people's health.
The various microbial deodorants being prepared in the prior art have the unconspicuous problem of deodorizing effect.
Summary of the invention
In view of the defects existing in the prior art, the present invention provides a kind of preparation method of microbial deodorant, can effectively solve
The certainly above problem.
The technical solution adopted by the invention is as follows:
The present invention provides a kind of preparation method of microbial deodorant, comprising the following steps:
The present invention provides a kind of preparation method of microbial deodorant, and the lactic acid bacteria first order seed being prepared is inoculated into
It further ferments in saccharomycetes to make fermentation liquid, microbial deodorant is finally prepared；The preparation method of microbial deodorant includes
Following steps:
Step 1, saccharomycete slant medium, the saccharomycete inclined-plane culture based component are prepared are as follows: mass fraction is 1%
Yeast extract, the peptone that mass fraction is 2%, the agar powder that the glucose and mass fraction that mass fraction is 2% are 2%；
Prepare lactic acid bacteria slant medium, the lactic acid bacteria inclined-plane culture based component are as follows: the peptone of 10g/L, 10g/L's
Beef extract, the diammonium hydrogen citrate of 2g/L, the glucose of 20g/L, 1.0mL Tween 80, the sodium acetate of 5.0g/L, the phosphorus of 2.0g/L
Sour hydrogen dipotassium, the magnesium sulfate of 0.5g/L, the manganese sulfate of 0.25g/L, the agar of 15g/L, initial pH 6.5；
Step 2, saccharomycete is inoculated in the saccharomycete slant medium that step 1 is prepared, in the condition of certain temperature
Lower activation, the saccharomycete after being activated；
The lactic acid bacteria slant medium that lactobacillus inoculum to step 1 is prepared, is activated under certain temperature, is obtained
Lactic acid bacteria after to activation；
Step 3, the saccharomycete after step 2 activation is inoculated in saccharomycete seed culture medium, under the conditions of certain temperature
36h~48h is cultivated, saccharomycete first order seed is prepared；
By the lactobacillus inoculum after step 2 activation in lactic acid bacteria seed culture medium, training is stood under the conditions of certain temperature
36h~48h is supported, until seed maturation, lactic acid bacteria first order seed is prepared；
Step 4, the saccharomycete first order seed that step 3 is prepared is inoculated into saccharomycete fluid nutrient medium by formula ratio
In, it ferments in the fermenter；Wherein, saccharomycete liquid medium component are as follows: the molasses or sucrose that mass fraction is 6~8%, matter
Measure the yeast extract that score is 0.5~1.5%；Inoculum concentration is 1.5~3%；Fermentation are as follows: tank presses 0.07~0.08MPa,
30~35 DEG C of temperature, fermentation time 36~48 hours, the saccharomycetes to make fermentation liquid fermented is prepared；
Step 5, the cream that the directly further fermentation step 3 of the saccharomycetes to make fermentation liquid being prepared using step 4 is prepared
Sour bacterium first order seed, is finally prepared microbial deodorant.
Preferably, in step 2, saccharomycete is inoculated in the saccharomycete slant medium that step 1 is prepared, in 30~35
It is activated under the conditions of DEG C；The lactic acid bacteria slant medium that lactobacillus inoculum to step 1 is prepared, lives in 35~37 DEG C
Change.
Preferably, in step 2, saccharomycete is inoculated in the saccharomycete slant medium that step 1 is prepared, in 30 DEG C of items
It is activated under part；The lactic acid bacteria slant medium that lactobacillus inoculum to step 1 is prepared, is activated in 37 DEG C.
Preferably, in step 3, the saccharomycete after step 2 activation is inoculated in saccharomycete seed culture medium, in 30~35
36h~48h is cultivated under the conditions of DEG C, and saccharomycete first order seed is prepared；Wherein saccharomycete seed medium component are as follows: quality point
The yeast extract that number is 1%, the peptone that mass fraction is 2%, the glucose that mass fraction is 2%；
By the lactobacillus inoculum after activation in lactic acid bacteria seed culture medium, the stationary culture 36h under the conditions of 35~37 DEG C
Lactic acid bacteria first order seed is prepared until seed maturation in~48h；Wherein, lactic acid bacteria seed medium component: mass fraction
For 5~8% molasses, the yeast extract that mass fraction is 1~2%, the peptone that mass fraction is 1%, mass fraction 0.5%
Sodium acetate, mass fraction be 0.2% dipotassium hydrogen phosphate, mass fraction be 0.058% magnesium sulfate, initial pH 6.5.
Preferably, in step 3, the saccharomycete after step 2 activation is inoculated in saccharomycete seed culture medium, in 30 DEG C of items
36h~48h is cultivated under part, and saccharomycete first order seed is prepared；
By the lactobacillus inoculum after activation in lactic acid bacteria seed culture medium, under the conditions of 37 DEG C stationary culture 36h~
Lactic acid bacteria first order seed is prepared until seed maturation in 48h.
Preferably, step 5 specifically:
The saccharomycetes to make fermentation liquid that step 4 is prepared is added in pail pack, the lactic acid bacteria one that step 3 is prepared
Grade seed is inoculated into saccharomycetes to make fermentation liquid and further ferments, fermentation are as follows: and pail pack pressure is normal pressure, temperature 25~
35 DEG C, fermentation time 60~96 hours, the inoculum concentration of lactic acid bacteria first order seed was 2~3%, and microbial deodorant is finally prepared
Agent.
Preferably, step 5 specifically:
Using saccharomycetes to make fermentation liquid directly further fermentative lactobacillus first order seed in the fermenter, it may be assumed that complete step 4
In the fermentor of the saccharomycetes to make fermentation liquid fermented, the lactic acid bacteria first order seed that direct inoculation step 3 obtains, inoculum concentration be 2~
3%, fermentation are as follows: temperature: 30~35 DEG C, fermentation time: 36~48 hours.
A kind of preparation method of microbial deodorant provided by the invention has the advantage that
The present invention provides a kind of preparation method of microbial deodorant, is that pollution-free, efficient, the safe microorganism of one kind removes
Smelly dose, by saccharomycete and lactic acid bacteria mixed fermentation, hence it is evident that the deodorizing effect for improving microbial deodorant solves traditional chemical and removes
The problem of smelly dose of deodorization is not thorough, and pollutes environment.
Detailed description of the invention
Fig. 1 is a kind of flow diagram of the preparation method of microbial deodorant provided by the invention.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Accompanying drawings and embodiments, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein only to
It explains the present invention, is not intended to limit the present invention.
The present invention provides a kind of preparation method of microbial deodorant, is that pollution-free, efficient, the safe microorganism of one kind removes
Smelly dose, by saccharomycete and lactic acid bacteria mixed fermentation, hence it is evident that the deodorizing effect for improving microbial deodorant solves traditional chemical and removes
The problem of smelly dose of deodorization is not thorough, and pollutes environment.
Embodiment one:
Strain source:
Saccharomycete is that our company screens from rubbish；Lactic acid bacteria is lactobacillus plantarum (Lactobacillus
Plantarum), it is purchased from Chinese industrial Microbiological Culture Collection administrative center (CICC), bacterium numbering 20764.
Step 1, saccharomycete slant medium, the saccharomycete inclined-plane culture based component are prepared are as follows: mass fraction is 1%
Yeast extract, the peptone that mass fraction is 2%, the agar powder that the glucose and mass fraction that mass fraction is 2% are 2%；
Prepare lactic acid bacteria slant medium, the lactic acid bacteria inclined-plane culture based component are as follows: the peptone of 10g/L, 10g/L's
Beef extract, the diammonium hydrogen citrate of 2g/L, the glucose of 20g/L, 1.0mL Tween 80, the sodium acetate of 5.0g/L, the phosphorus of 2.0g/L
Sour hydrogen dipotassium, the magnesium sulfate of 0.5g/L, the manganese sulfate of 0.25g/L, the agar of 15g/L, initial pH 6.5；
Step 2, saccharomycete is inoculated in the saccharomycete slant medium that step 1 is prepared, it is living under conditions of 30 DEG C
After changing 20h, the saccharomycete after being activated；
The lactic acid bacteria slant medium that lactobacillus inoculum to step 1 is prepared is activated under the conditions of 37 DEG C, is obtained
Lactic acid bacteria after to activation；
Step 3, the saccharomycete after activation is inoculated in saccharomycete seed culture medium, cultivates 36h under the conditions of 30 DEG C, until
Seed is mature, and saccharomycete first order seed is prepared；Wherein saccharomycete seed medium component are as follows: the ferment that mass fraction is 1%
Female cream, the peptone that mass fraction is 2%, the glucose that mass fraction is 2%；
By the lactobacillus inoculum after activation in lactic acid bacteria seed culture medium, stationary culture 40h under the conditions of 30 DEG C, until kind
It is sub mature, lactic acid bacteria first order seed is prepared；Wherein, lactic acid bacteria seed medium component: the molasses that mass fraction is 7%,
The yeast extract that mass fraction is 1.5%, the peptone that mass fraction is 1%, the sodium acetate that mass fraction is 0.5%, quality point
The dipotassium hydrogen phosphate that number is 0.2%, the magnesium sulfate that mass fraction is 0.058%, initial pH 6.5；
Step 4, saccharomycete first order seed is inoculated into saccharomycete fluid nutrient medium by formula ratio, is sent out in the fermenter
Ferment；Wherein, saccharomycete liquid medium component: the molasses or sucrose that mass fraction is 6%, the yeast that mass fraction is 0.5%
Cream, inoculum concentration 2.5%, initial pH 6.5；Fermentation are as follows: tank press 0.07MPa, 31 DEG C of temperature, speed of agitator
Fermentation time 36 hours, the saccharomycetes to make fermentation liquid fermented was prepared in 200rpm, ventilation quantity 1:0.8；
Step 5, saccharomycetes to make fermentation liquid step 4 being prepared is added in pail pack, the cream that step 3 is prepared
Sour bacterium first order seed, which is inoculated into saccharomycetes to make fermentation liquid, further to ferment, and herein, is directly further sent out using saccharomycetes to make fermentation liquid
Ferment lactic acid bacteria；Wherein, fermentation are as follows: pail pack pressure be normal pressure, 35 DEG C of temperature, fermentation time 60 hours, lactic acid bacteria
The inoculum concentration of first order seed is 2%, and microbial deodorant is finally prepared.
Embodiment two:
Saccharomycete is that our company screens from rubbish；Lactic acid bacteria is lactobacillus plantarum (Lactobacillus
Plantarum), it is purchased from Chinese industrial Microbiological Culture Collection administrative center (CICC), bacterium numbering 20764.
Step 1, saccharomycete slant medium, the saccharomycete inclined-plane culture based component are prepared are as follows: mass fraction is 1%
Yeast extract, the peptone that mass fraction is 2%, the agar powder that the glucose and mass fraction that mass fraction is 2% are 2%；
Prepare lactic acid bacteria slant medium, the lactic acid bacteria inclined-plane culture based component are as follows: the peptone of 10g/L, 10g/L's
Beef extract, the diammonium hydrogen citrate of 2g/L, the glucose of 20g/L, 1.0mL Tween 80, the sodium acetate of 5.0g/L, the phosphorus of 2.0g/L
Sour hydrogen dipotassium, the magnesium sulfate of 0.5g/L, the manganese sulfate of 0.25g/L, the agar of 15g/L, initial pH 6.5；
Step 2, saccharomycete is inoculated in the saccharomycete slant medium that step 1 is prepared, it is living under conditions of 30 DEG C
After changing 20h, the saccharomycete after being activated；
The lactic acid bacteria slant medium that lactobacillus inoculum to step 1 is prepared is activated under the conditions of 37 DEG C, is obtained
Lactic acid bacteria after to activation；
Step 3, the saccharomycete after activation is inoculated in saccharomycete seed culture medium, cultivates 48h under the conditions of 30 DEG C, until
Seed is mature, and saccharomycete first order seed is prepared；Wherein saccharomycete seed medium component are as follows: the ferment that mass fraction is 1%
Female cream, the peptone that mass fraction is 2%, the glucose that mass fraction is 2%；
By the lactobacillus inoculum after activation in lactic acid bacteria seed culture medium, stationary culture 36h~48h under the conditions of 37 DEG C,
Lactic acid bacteria first order seed is prepared；Wherein, lactic acid bacteria seed medium component: the molasses that mass fraction is 5%, mass fraction
For 1.5% yeast extract, the peptone that mass fraction is 1%, the sodium acetate that mass fraction is 0.5%, mass fraction 0.2%
Dipotassium hydrogen phosphate, mass fraction be 0.058% magnesium sulfate, initial pH 6.5；
Step 4, saccharomycete first order seed is inoculated into saccharomycete fluid nutrient medium by formula ratio, is sent out in the fermenter
Ferment；Wherein, saccharomycete liquid medium component: the molasses or sucrose that mass fraction is 8%, the yeast that mass fraction is 0.5%
Cream, inoculum concentration are 2.5% mass fraction, initial pH 6.5；Fermentation are as follows: tank presses 0.08MPa, 30 DEG C of temperature, stirs
Fermentation time 36 hours, the saccharomycetes to make fermentation liquid fermented was prepared in revolving speed 200rpm, ventilation quantity 1:0.8；
Step 5, saccharomycetes to make fermentation liquid step 4 being prepared is added in pail pack, the cream that step 3 is prepared
Sour bacterium first order seed, which is inoculated into saccharomycetes to make fermentation liquid, further to ferment, and herein, is directly further sent out using saccharomycetes to make fermentation liquid
Ferment lactic acid bacteria；Wherein, fermentation are as follows: pail pack pressure be normal pressure, 30 DEG C of temperature, fermentation time 96 hours, lactic acid bacteria
The inoculum concentration of first order seed is 3%, and microbial deodorant is finally prepared.
Total bacteria count > 10 for the microbial deodorant that embodiment one and embodiment two are prepared9CFU/mL。
The preparation method of microbial deodorant provided by the invention, deodorant production method is simple, time saving and energy saving, due to adopting
With lactic acid bacteria and saccharomycete mixed fermentation, directly lactic acid bacteria first order seed is inoculated into saccharomycetes to make fermentation liquid and is fermented, lactic acid bacteria
Mutually promote with saccharomycete, on the one hand, saccharomycete can secretion activity substance, promote beneficial microbe growth, itself can produce
Raw fragrance；On the other hand, lactic acid bacteria is able to carry out organic matter fermentation, adjusts pH, inhibits harmful bacteria growth；Also, lactic acid bacteria and
Saccharomycete, which is mutually promoted, generates unexpected technical effect, experiment proves that, the microbial deodorant that the present invention is prepared,
Its deodorizing effect is apparently higher than individual saccharomycetes to make fermentation liquid or lactobacillus solution.
Inspection example 1
This inspection example is used to investigate the deodorizing capability of microbial bacterial agent.
The microbial deodorant 200mL that the embodiment of the present invention 1 is prepared dilutes 20 times of mixings, system with deionized water
At deodorization bacterium solution:
Landfill leachate is taken, 5L is respectively taken, is put into two 25L plastic barrels, one experimental group of a control group, experimental group is every
Every three hours sprinkling microbial bacterial agents, until all having sprayed deodorant, control group sprayed same amount of deionized water, examined after 48h
Survey ammonia and concentration of hydrogen sulfide.
The experimental results showed that deodorization bacterium solution is after spraying, because microbial deodorant is acidity, can capture in air rapidly
Ammonia reduces stink.
Through detecting, compared with the control group, experimental group ammonia concentration declines experimental group than control group ammonia concentration after 48h
70%, experimental group concentration of hydrogen sulfide is than control group concentration of hydrogen sulfide decline 50%, hence it is evident that inhibits the generation of stink.
Inspection example 2
This inspection example is used to investigate the deodorizing capability of microbial bacterial agent.
Implement live deodorizing test in refuse landfill, meteorological condition is 25 degree of temperature, 3 grades of wind speed or less.In operation area
A sampled point is laid at domain center, sprays the microbial deodorant 500ml that embodiment two is prepared, dilution spray by one square metre
After spilling implementation, the rate of descent of 2 hours and 6 hours detection hydrogen sulfide and ammonia compared with before sprinkling, to detect deodorizing effect.
It can be seen that the microbial deodorant that the present invention uses, when carrying out deodorization to house refuse, have it is quick and
Deodorizing effect clear advantage.
Reference examples 1
Control experiment:
Check experiment 1: saccharomycetes to make fermentation deodorant is prepared:
Step 1, saccharomycete slant medium, the saccharomycete inclined-plane culture based component are prepared are as follows: mass fraction is 1%
Yeast extract, the peptone that mass fraction is 2%, the agar powder that the glucose and mass fraction that mass fraction is 2% are 2%；
Step 2, saccharomycete is inoculated in the saccharomycete slant medium that step 1 is prepared, it is living under conditions of 30 DEG C
After changing 20h, the saccharomycete after being activated；
Step 3, the saccharomycete after activation is inoculated in saccharomycete seed culture medium, cultivates 48h under the conditions of 30 DEG C, until
Seed is mature, and saccharomycete first order seed is prepared；Wherein saccharomycete seed medium component are as follows: the ferment that mass fraction is 1%
Female cream, the peptone that mass fraction is 2%, the glucose that mass fraction is 2%；
Step 4, saccharomycete first order seed is inoculated into saccharomycete fluid nutrient medium by formula ratio, is sent out in the fermenter
Ferment；Wherein, saccharomycete liquid medium component: the molasses or sucrose that mass fraction is 6%, the yeast that mass fraction is 0.5%
Cream, inoculum concentration 2.5%, initial pH 6.5；Fermentation are as follows: tank press 0.08MPa, 33 DEG C of temperature, speed of agitator
Fermentation time 36 hours, the saccharomycetes to make fermentation liquid fermented is prepared, as saccharomycetes to make fermentation in 200rpm, ventilation quantity 1:0.8
Deodorant.
Check experiment 2: lactobacillus-fermented deodorant is prepared:
Step 1, lactic acid bacteria slant medium, the lactic acid bacteria inclined-plane culture based component are prepared are as follows: the peptone of 10g/L,
The beef extract of 10g/L, the diammonium hydrogen citrate of 2g/L, the glucose of 20g/L, 1.0mL Tween 80, the sodium acetate of 5.0g/L,
The dipotassium hydrogen phosphate of 2.0g/L, the magnesium sulfate of 0.5g/L, the manganese sulfate of 0.25g/L, the agar of 15g/L, initial pH 6.5；
Step 2, the lactic acid bacteria slant medium that lactobacillus inoculum to step 1 is prepared is lived under the conditions of 37 DEG C
Change, the lactic acid bacteria after being activated；
Step 3, by the lactobacillus inoculum after activation in lactic acid bacteria seed culture medium, stationary culture 36h under the conditions of 37 DEG C
Lactic acid bacteria first order seed is prepared until seed maturation in~48h；Wherein, lactic acid bacteria seed medium component: mass fraction
For 7% molasses, the yeast extract that mass fraction is 1.5%, the peptone that mass fraction is 1%, the second that mass fraction is 0.5%
Sour sodium, the dipotassium hydrogen phosphate that mass fraction is 0.2%, the magnesium sulfate that mass fraction is 0.058%, initial pH 6.5；Lactic acid bacteria
First order seed is used as lactic acid bacteria deodorant.
Saccharomycetes to make fermentation deodorant prepared by check experiment 1 is as check sample 1；Lactic acid prepared by check experiment 2
The agent of bacterium fermentative deodorizing is as check sample 2；The microbial deodorant that the embodiment of the present invention one is prepared as test sample,
Carry out following check experiment:
Test sample, check sample 1 and check sample 2 are respectively taken into 200mL, dilute 20 times of mixings with deionized water, respectively
Deodorization bacterium solution is made:
Landfill leachate is taken, 5L is respectively taken, is put into three 25L plastic barrels, every three hours, is respectively sprayed into each bucket
Test sample, check sample 1 and check sample 2 have been sprayed until all, and ammonia and concentration of hydrogen sulfide are detected after 48h.
Through detecting, for test sample, ammonia concentration declines than decline 70% before sprinkling, concentration of hydrogen sulfide after 48h
50%, hence it is evident that inhibit the generation of stink.
For ammonia after check sample Isosorbide-5-Nitrae 8h than concentration decline 40% before sprinkling, concentration of hydrogen sulfide declines 30%,
For check sample 2, ammonia declines 15% than concentration before sprinkling after 48h, and concentration of hydrogen sulfide declines 5%,
It can be seen that the deodorizing capability of test sample of the present invention, hence it is evident that be higher than individual saccharomycetes to make fermentation deodorant and cream
Acid bacteria fermentation deodorant, also, the deodorizing capability of test sample of the present invention are greater than saccharomycetes to make fermentation deodorant and lactobacillus-fermented
The sum of the deodorizing effect of deodorant, simple superposition both not.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
Depending on protection scope of the present invention.
Claims (7)
1. a kind of preparation method of microbial deodorant, which is characterized in that the lactic acid bacteria first order seed being prepared to be inoculated into
It further ferments in saccharomycetes to make fermentation liquid, microbial deodorant is finally prepared；The preparation method of microbial deodorant includes
Following steps:
Step 1, saccharomycete slant medium, the saccharomycete inclined-plane culture based component are prepared are as follows: the yeast that mass fraction is 1%
Cream, the peptone that mass fraction is 2%, the agar powder that the glucose and mass fraction that mass fraction is 2% are 2%；
Prepare lactic acid bacteria slant medium, the lactic acid bacteria inclined-plane culture based component are as follows: the peptone of 10g/L, the beef of 10g/L
Cream, the diammonium hydrogen citrate of 2g/L, the glucose of 20g/L, 1.0mL Tween 80, the sodium acetate of 5.0g/L, the phosphoric acid hydrogen of 2.0g/L
Dipotassium, the magnesium sulfate of 0.5g/L, the manganese sulfate of 0.25g/L, the agar of 15g/L, initial pH6.5；
Step 2, saccharomycete is inoculated in the saccharomycete slant medium that step 1 is prepared, it is living under conditions of certain temperature
Change, the saccharomycete after being activated；
The lactic acid bacteria slant medium that lactobacillus inoculum to step 1 is prepared, is activated under certain temperature, is lived
Lactic acid bacteria after change；
Step 3, the saccharomycete after step 2 activation is inoculated in saccharomycete seed culture medium, is cultivated under the conditions of certain temperature
Saccharomycete first order seed is prepared in 36h~48h；
By the lactobacillus inoculum after step 2 activation in lactic acid bacteria seed culture medium, the stationary culture 36h under the conditions of certain temperature
Lactic acid bacteria first order seed is prepared until seed maturation in~48h；
Step 4, the saccharomycete first order seed that step 3 is prepared is inoculated into saccharomycete fluid nutrient medium by formula ratio,
Fermentation cylinder for fermentation；Wherein, saccharomycete liquid medium component are as follows: the molasses or sucrose that mass fraction is 6~8%, quality point
The yeast extract that number is 0.5~1.5%；Inoculum concentration is 1.5~3%；Fermentation are as follows: tank presses 0.07~0.08MPa, temperature
30~35 DEG C, fermentation time 36~48 hours, the saccharomycetes to make fermentation liquid fermented is prepared；
Step 5, the lactic acid bacteria that the directly further fermentation step 3 of the saccharomycetes to make fermentation liquid being prepared using step 4 is prepared
Microbial deodorant is finally prepared in first order seed.
2. the preparation method of microbial deodorant according to claim 1, which is characterized in that in step 2, saccharomycete is connect
The saccharomycete slant medium that kind is prepared in step 1, activates under the conditions of 30~35 DEG C；By lactobacillus inoculum to step 1
The lactic acid bacteria slant medium being prepared, is activated in 35~37 DEG C.
3. the preparation method of microbial deodorant according to claim 2, which is characterized in that in step 2, saccharomycete is connect
The saccharomycete slant medium that kind is prepared in step 1, activates under the conditions of 30 DEG C；It prepared by lactobacillus inoculum to step 1
Obtained lactic acid bacteria slant medium, is activated in 37 DEG C.
4. the preparation method of microbial deodorant according to claim 1, which is characterized in that in step 3, step 2 is living
Saccharomycete after change is inoculated in saccharomycete seed culture medium, and 36h~48h is cultivated under the conditions of 30~35 DEG C, ferment is prepared
Female bacterium first order seed；Wherein saccharomycete seed medium component are as follows: the yeast extract that mass fraction is 1%, mass fraction are 2%
Peptone, the glucose that mass fraction is 2%；
By the lactobacillus inoculum after activation in lactic acid bacteria seed culture medium, under the conditions of 35~37 DEG C stationary culture 36h~
Lactic acid bacteria first order seed is prepared until seed maturation in 48h；Wherein, lactic acid bacteria seed medium component: mass fraction 5
~8% molasses, the yeast extract that mass fraction is 1~2%, the peptone that mass fraction is 1%, mass fraction are 0.5%
Sodium acetate, the dipotassium hydrogen phosphate that mass fraction is 0.2%, the magnesium sulfate that mass fraction is 0.058%, initial pH6.5.
5. the preparation method of microbial deodorant according to claim 4, which is characterized in that in step 3, step 2 is living
Saccharomycete after change is inoculated in saccharomycete seed culture medium, and 36h~48h is cultivated under the conditions of 30 DEG C, saccharomycete is prepared
First order seed；
By the lactobacillus inoculum after activation in lactic acid bacteria seed culture medium, stationary culture 36h~48h under the conditions of 37 DEG C, directly
To seed maturation, lactic acid bacteria first order seed is prepared.
6. the preparation method of microbial deodorant according to claim 1, which is characterized in that step 5 specifically:
The saccharomycetes to make fermentation liquid that step 4 is prepared is added in pail pack, the lactic acid bacteria level-one kind that step 3 is prepared
Son, which is inoculated into saccharomycetes to make fermentation liquid, further to ferment, fermentation are as follows: pail pack pressure is normal pressure, temperature 25~35
DEG C, fermentation time 60~96 hours, the inoculum concentration of lactic acid bacteria first order seed was 2~3%, and microbial deodorant is finally prepared
Agent.
7. the preparation method of microbial deodorant according to claim 1, which is characterized in that step 5 specifically:
Using saccharomycetes to make fermentation liquid directly further fermentative lactobacillus first order seed in the fermenter, it may be assumed that complete step 4 fermentation
In the fermentor of good saccharomycetes to make fermentation liquid, the lactic acid bacteria first order seed that direct inoculation step 3 obtains, inoculum concentration is 2~3%,
Fermentation are as follows: temperature: 30~35 DEG C, fermentation time: 36~48 hours.
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| CN102327630A (en) * | 2011-09-15 | 2012-01-25 | 湖南省微生物研究所 | Microbial strain and deodorant for municipal landfill, and preparation and application method thereof |
| CN104548173A (en) * | 2014-12-24 | 2015-04-29 | 湖南科美洁环保科技有限公司 | Preparation method for microbial garbage deodorizer |
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